LABORATORY OF BIOMOLECULAR ENGINEERING AND NANOMEDICINE
Principal Investigator: Gang Bao
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TAL DESIGN & ANALYSIS GROUP
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Tutorials
Step-by-step examples for each tool using the sample data set.
TAL Plasmids Assembly Tool
Generating maps for TALE and TALEN plasmids assembled through different methods:
Golden Gate (Plasmid Based)
described by Cermak et al.
Iterative Capped Assembly (ICA)
described by Briggs et al.
Fast Ligation-based Automatable Solid-phase High-throughput (FLASH)
described by Reyon & Tsai et al.
Automatic alignment of sequencing reads.
Predicted Report of Genome-wide Nuclease Off-target Sites (PROGNOS)
Identify and test potential nuclease off-target sites.
Using the
PROGNOS Input Form
to specify your search criteria.
Interpreting
PROGNOS Output
to understand the results.
Scoring Algorithm for Predicted TALE(N) Activity (SAPTA)
Find TALE and TALEN binding sites in your gene of interest predicted to have high activity.
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