PROGNOS Summary for 12212012SXJ93rlv_QUEUE_Ranking-RVDs_5TC

The hg19 genome was searched for the TALEN pair targeting:
5'- TTTGTGGGCAACATGCTGG NN..NN ATAAACTGCAAAAGGCTGA -3'

Using these RVDs:
Left:  01NG02NG03NN04NG05NN06NN07NN08HD09NI10NI11HD12NI13NG14NN15HD16NG17NN18NN
Right: 01HD02NI03NN04HD05HD06NG07NG08NG09NG10NN11HD12NG13NN14NG15NG16NG17NI18NG

Up to 6 mismatches were allowed in each nuclease half-site.

Allowed spacing distances were: 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30

Homodimers and heterodimers of the nucleases were included in the output

2966 total sites were located
Mismatch Types
Genomic Regions
  0-0: 1
  0-1: 1
  3-5: 4
  4-4: 2
  3-6: 18
  4-5: 13
  4-6: 86
  5-5: 63
  5-6: 691
  6-6: 2087
  Exons: 68
  Promoters: 11
  Introns: 1138
  Intergenic: 1749


The top 48 sites are ranked below according to a weighting of the RVD base preferences. All sites listed contain flanking 5' pyrimidines.
For the full output, click here.

This output will be deleted from the server one week after it is generated.  Please save your results.

Primer pairs were designed using the 422 bp of sequence on either side of the cleavage site to melt close to 60 °C

For easy visualization of cleavage on gels:
The smallest fragment will be longer than 125 bp, which is also the minimum separation distance between uncleaved and cleaved products.
The two cleavage products will be separated by a minimum size of 75 bp
Rank: 1	Homology Score: 98	RVD Match Score: 2	Left Half-Site RVD Score: 4.024	Right Half-Site RVD Score: 3.881
	Genomic Region: Exon	Closest Gene: CCR5
Match Type: L14R
	           Left Nuclease
	         TTTGTGGGCAACATGCTGG
	5'-TTGGT TTTGTGGGCAACATGCTGG         TCATCCTCATCCTG         ATAAACTGCAAAAGGCTGA AGAGC-3'
	3'-AACCA AAACACCCGTTGTACGACC         AGTAGGAGTAGGAC         TATTTGACGTTTTCCGACT TCTCG-5'
	                                                            TATTTGACGTTTTCCGACT
	                                                                  Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 003-046414377
Forward Primer: 5'-GCACAGGGTGGAACAAGATGG-3' Tm: 59.378
Reverse Primer: 5'-GTGACCGTCCTGGCTTTTAAAGCA-3' Tm: 59.038
Expected PCR Product Length: 446
Expected CelI Cleavage Product Sizes: 177, 269
In Silico PCR
Genome Browser

Rank: 2	Homology Score: 85	RVD Match Score: 2.14	Left Half-Site RVD Score: 4.024	Right Half-Site RVD Score: 5.045
	Genomic Region: Exon	Closest Gene: CCR2
Match Type: L14R
	           Left Nuclease
	         TTTGTGGGCAACATGCTGG
	5'-TTGGT TTTGTGGGCAACATGCTGG         TCGTCCTCATCTTA         ATAAACTGCAAAAAGCTGA AGTGC-3'
	3'-AACCA AAACACCCGTTGTACGACC         AGCAGGAGTAGAAT         TATTTGACGTTTTTCGACT TCACG-5'
	                                                            TATTTGACGTTTTCCGACT
	                                                                  Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 003-046399009
Forward Primer: 5'-CATCCACAACATGCTGTCCACATC-3' Tm: 59.038
Reverse Primer: 5'-CAAAGACCCACTCATTTGCAGCAG-3' Tm: 59.038
Expected PCR Product Length: 336
Expected CelI Cleavage Product Sizes: 206, 130
In Silico PCR
Genome Browser

Rank: 3	Homology Score: 10	RVD Match Score: 2.376	Left Half-Site RVD Score: 5.159	Right Half-Site RVD Score: 5.814
	Genomic Region: Intron	Closest Gene: ARSB
Match Type: R11R
	          Right Nuclease
	         TCAGCCTTTTGCAGTTTAT
	5'-AACAC CCAGCCTTTTCCTAATAAT          TGCTGAGTCGC           ATAACAAGCAAAAGGCTTA ATCCT-3'
	3'-TTGTG GGTCGGAAAAGGATTATTA          ACGACTCAGCG           TATTGTTCGTTTTCCGAAT TAGGA-5'
	                                                            TATTTGACGTTTTCCGACT
	                                                                  Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 005-078100805
Forward Primer: 5'-TAGCAGGGACACAGCATCCG-3' Tm: 59.508
Reverse Primer: 5'-CTCGTTGGCTGACTTATGCTCTG-3' Tm: 59.148
Expected PCR Product Length: 343
Expected CelI Cleavage Product Sizes: 212, 131
In Silico PCR
Genome Browser

Rank: 4	Homology Score: 17	RVD Match Score: 2.385	Left Half-Site RVD Score: 5.561	Right Half-Site RVD Score: 5.682
	Genomic Region: Intergenic	Closest Gene: FLJ42709
Match Type: R12L
	          Right Nuclease
	         TCAGCCTTTTGCAGTTTAT
	5'-ATATT CCAGCCTTTTCTTATTTCT          ATTCCATCCCTG          TGAACATGTTGCCCACAAA GTAGG-3'
	3'-TATAA GGTCGGAAAAGAATAAAGA          TAAGGTAGGGAC          ACTTGTACAACGGGTGTTT CATCC-5'
	                                                            GGTCGTACAACGGGTGTTT
	                                                                   Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 005-091821506
Forward Primer: 5'-AGGACTGGCTCCTTGTGGTG-3' Tm: 59.508
Reverse Primer: 5'-GACCTGCTTCTCTGGTCTTTTCTG-3' Tm: 59.038
Expected PCR Product Length: 563
Expected CelI Cleavage Product Sizes: 126, 437
In Silico PCR
Genome Browser

Rank: 5	Homology Score: 5	RVD Match Score: 2.446	Left Half-Site RVD Score: 5.764	Right Half-Site RVD Score: 6.281
	Genomic Region: Intron	Closest Gene: DMXL1
Match Type: L26L
	           Left Nuclease
	         TTTGTGGGCAACATGCTGG
	5'-TAGGT CTTCTGGATAACATGCTGA   AGCTTCTACGTCATTCAGCACTTGCT   TCAGCATGTTTTCCAGAGG ATCTA-3'
	3'-ATCCA GAAGACCTATTGTACGACT   TCGAAGATGCAGTAAGTCGTGAACGA   AGTCGTACAAAAGGTCTCC TAGAT-5'
	                                                            GGTCGTACAACGGGTGTTT
	                                                                   Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 005-118490138
Forward Primer: 5'-GTCTAGATGGATCGAACTAGCCAC-3' Tm: 59.038
Reverse Primer: 5'-GCACTGACTGCTCTCTAACTACG-3' Tm: 59.148
Expected PCR Product Length: 390
Expected CelI Cleavage Product Sizes: 236, 154
In Silico PCR
Genome Browser

Rank: 6	Homology Score: 17	RVD Match Score: 2.477	Left Half-Site RVD Score: 6.106	Right Half-Site RVD Score: 6.023
	Genomic Region: Intron	Closest Gene: VEPH1
Match Type: R29L
	          Right Nuclease
	         TCAGCCTTTTGCAGTTTAT
	5'-TCCAG TCAAATTTTAGCTATTTAT CCATTGCTGTGGTCCTCTGACCCCTTGGG  CCAGCAGGTTGGCCACATA GGCAC-3'
	3'-AGGTC AGTTTAAAATCGATAAATA GGTAACGACACCAGGAGACTGGGGAACCC  GGTCGTCCAACCGGTGTAT CCGTG-5'
	                                                            GGTCGTACAACGGGTGTTT
	                                                                   Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 003-157066450
Forward Primer: 5'-AAGGAAGAGGGGGCTGCAGA-3' Tm: 59.508
Reverse Primer: 5'-GCTGAGTCCTTGTCTGGTTTTGG-3' Tm: 59.148
Expected PCR Product Length: 388
Expected CelI Cleavage Product Sizes: 153, 235
In Silico PCR
Genome Browser

Rank: 7	Homology Score: 5	RVD Match Score: 2.513	Left Half-Site RVD Score: 6.204	Right Half-Site RVD Score: 6.058
	Genomic Region: Intron	Closest Gene: CHN1
Match Type: R27R
	          Right Nuclease
	         TCAGCCTTTTGCAGTTTAT
	5'-ATACC CCCGCCTTTTGGTGTTTAG  GCACAACTGACCAGCATTAACATTGAA   ATAGAGATTATAAGGCTGA CAACA-3'
	3'-TATGG GGGCGGAAAACCACAAATC  CGTGTTGACTGGTCGTAATTGTAACTT   TATCTCTAATATTCCGACT GTTGT-5'
	                                                            TATTTGACGTTTTCCGACT
	                                                                  Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 002-175789857
Forward Primer: 5'-GACTCCCTCTTGGCCAACAG-3' Tm: 59.508
Reverse Primer: 5'-CAGTTGCAAATTCCCCCACAAGG-3' Tm: 59.148
Expected PCR Product Length: 331
Expected CelI Cleavage Product Sizes: 127, 204
In Silico PCR
Genome Browser

Rank: 8	Homology Score: 17	RVD Match Score: 2.519	Left Half-Site RVD Score: 4.677	Right Half-Site RVD Score: 8.139
	Genomic Region: Intergenic	Closest Gene: AP1AR
Match Type: R18L
	          Right Nuclease
	         TCAGCCTTTTGCAGTTTAT
	5'-AATTT TCAGCTTTTTCCTGTTTAT       CGTGATACGAATATTCCA       ACAGCATGTTAAACAAAAG ACGTT-3'
	3'-TTAAA AGTCGAAAAAGGACAAATA       GCACTATGCTTATAAGGT       TGTCGTACAATTTGTTTTC TGCAA-5'
	                                                            GGTCGTACAACGGGTGTTT
	                                                                   Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 004-113138626
Forward Primer: 5'-GCCATCCAAATGTGGACAGTAGG-3' Tm: 59.148
Reverse Primer: 5'-GTAGGGGAAACCAATTACAGTGCC-3' Tm: 59.038
Expected PCR Product Length: 394
Expected CelI Cleavage Product Sizes: 147, 247
In Silico PCR
Genome Browser

Rank: 9	Homology Score: 8	RVD Match Score: 2.521	Left Half-Site RVD Score: 7.061	Right Half-Site RVD Score: 5.561
	Genomic Region: Intron	Closest Gene: MAP4K3
Match Type: L30R
	           Left Nuclease
	         TTTGTGGGCAACATGCTGG
	5'-TGGGT TTGGTGGAAAAAATACTGG GAACCCTCCTACTCTCAACCAACATGAGGA AAAAACAGTAAAAGACTGG CACAG-3'
	3'-ACCCA AACCACCTTTTTTATGACC CTTGGGAGGATGAGAGTTGGTTGTACTCCT TTTTTGTCATTTTCTGACC GTGTC-5'
	                                                            TATTTGACGTTTTCCGACT
	                                                                  Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 002-039561318
Forward Primer: 5'-CCCTCTGCATGATCTGGTTGCATT-3' Tm: 59.038
Reverse Primer: 5'-GTAGTATCAGTGGGTGGGTGC-3' Tm: 59.378
Expected PCR Product Length: 339
Expected CelI Cleavage Product Sizes: 214, 125
In Silico PCR
Genome Browser

Rank: 10	Homology Score: 17	RVD Match Score: 2.521	Left Half-Site RVD Score: 6.771	Right Half-Site RVD Score: 5.6
	Genomic Region: Intergenic	Closest Gene: MIR563
Match Type: R28R
	          Right Nuclease
	         TCAGCCTTTTGCAGTTTAT
	5'-GCATT CTAACCAATTGCAATTTAT  GAACCAATTTGTTTTCCAATTTGAATAA  ATAAACTGAAAAAGTTTGA GACAG-3'
	3'-CGTAA GATTGGTTAACGTTAAATA  CTTGGTTAAACAAAAGGTTAAACTTATT  TATTTGACTTTTTCAAACT CTGTC-5'
	                                                            TATTTGACGTTTTCCGACT
	                                                                  Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 003-015921847
Forward Primer: 5'-GCTCTAGAACTCTAGACTGTGGG-3' Tm: 59.148
Reverse Primer: 5'-CTCACGGCCAATCCTGTTTTATCC-3' Tm: 59.038
Expected PCR Product Length: 412
Expected CelI Cleavage Product Sizes: 165, 247
In Silico PCR
Genome Browser

Rank: 11	Homology Score: 2	RVD Match Score: 2.525	Left Half-Site RVD Score: 6.985	Right Half-Site RVD Score: 5.439
	Genomic Region: Intergenic	Closest Gene: CHRDL1
Match Type: R30R
	          Right Nuclease
	         TCAGCCTTTTGCAGTTTAT
	5'-AAGAG TCAACTATGTGCTGTCTAT ATGAAACCCAGTTTAATATAAAAACACAGG TTAAAAAGTAAAAGGATGG AAGAT-3'
	3'-TTCTC AGTTGATACACGACAGATA TACTTTGGGTCAAATTATATTTTTGTGTCC AATTTTTCATTTTCCTACC TTCTA-5'
	                                                            TATTTGACGTTTTCCGACT
	                                                                  Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 00X-109896865
Forward Primer: 5'-CCATTGTACAGAGTGGTAACTCCAGTG-3' Tm: 60.438
Reverse Primer: 5'-GTTAGGGGGGAGTCCCTTCT-3' Tm: 59.508
Expected PCR Product Length: 433
Expected CelI Cleavage Product Sizes: 284, 149
In Silico PCR
Genome Browser

Rank: 12	Homology Score: 2	RVD Match Score: 2.536	Left Half-Site RVD Score: 6.343	Right Half-Site RVD Score: 6.368
	Genomic Region: Intron	Closest Gene: CNST
Match Type: R30L
	          Right Nuclease
	         TCAGCCTTTTGCAGTTTAT
	5'-TGTTT TCCATCTTTTGCTCTTTAC AAACTGGTTAAGACTGTTTCCCTCTCAGTG TCAGCAAGGTGCTTATAAA ACCTG-3'
	3'-ACAAA AGGTAGAAAACGAGAAATG TTTGACCAATTCTGACAAAGGGAGAGTCAC AGTCGTTCCACGAATATTT TGGAC-5'
	                                                            GGTCGTACAACGGGTGTTT
	                                                                   Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 001-246821429
Forward Primer: 5'-CAGCCAGATTCAAGATCCTCTCC-3' Tm: 59.148
Reverse Primer: 5'-GCTTCCCTGAATGTGGGTCTATAG-3' Tm: 59.038
Expected PCR Product Length: 394
Expected CelI Cleavage Product Sizes: 143, 251
In Silico PCR
Genome Browser

Rank: 13	Homology Score: 5	RVD Match Score: 2.538	Left Half-Site RVD Score: 7.14	Right Half-Site RVD Score: 5.422
	Genomic Region: Intron	Closest Gene: ANLN
Match Type: R29R
	          Right Nuclease
	         TCAGCCTTTTGCAGTTTAT
	5'-TTCTG TCAGTCTTATGATCTCTAT TTAACATTAATGCTCATCAGTTATTGTGT  TTAAACAGCAAAAGGAGGG GTATA-3'
	3'-AAGAC AGTCAGAATACTAGAGATA AATTGTAATTACGAGTAGTCAATAACACA  AATTTGTCGTTTTCCTCCC CATAT-5'
	                                                            TATTTGACGTTTTCCGACT
	                                                                  Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 007-036433669
Forward Primer: 5'-ATCCTTTTGCTTCAGCCACCCC-3' Tm: 59.258
Reverse Primer: 5'-AGATCCCCTCTTGATGAAGGGGAC-3' Tm: 60.908
Expected PCR Product Length: 326
Expected CelI Cleavage Product Sizes: 200, 126
In Silico PCR
Genome Browser

Rank: 14	Homology Score: 5	RVD Match Score: 2.553	Left Half-Site RVD Score: 5.992	Right Half-Site RVD Score: 6.916
	Genomic Region: Intron	Closest Gene: ZNF280D
Match Type: R16L
	          Right Nuclease
	         TCAGCCTTTTGCAGTTTAT
	5'-TGTGC CCAGCCTGTTTCTGTGTAT        TTAAAAAATTTTTTTT        CTAGAATGTTAGTCAGAAA AATAT-3'
	3'-ACACG GGTCGGACAAAGACACATA        AATTTTTTAAAAAAAA        GATCTTACAATCAGTCTTT TTATA-5'
	                                                            GGTCGTACAACGGGTGTTT
	                                                                   Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 015-056932411
Forward Primer: 5'-CAAGCCATATTGAAGCCTGAGGCA-3' Tm: 59.038
Reverse Primer: 5'-GCATTTGCTTCCATTAGCCAGGAG-3' Tm: 59.038
Expected PCR Product Length: 373
Expected CelI Cleavage Product Sizes: 233, 140
In Silico PCR
Genome Browser

Rank: 15	Homology Score: 5	RVD Match Score: 2.554	Left Half-Site RVD Score: 5.981	Right Half-Site RVD Score: 6.69
	Genomic Region: Intergenic	Closest Gene: LINC00184
Match Type: R17R
	          Right Nuclease
	         TCAGCCTTTTGCAGTTTAT
	5'-CTGGG TCAGTCTTTTAATTTTTAT       GAGTTAGTATGCCTCTT        GTAGACAGCATAAAGTTGA ATTTT-3'
	3'-GACCC AGTCAGAAAATTAAAAATA       CTCAATCATACGGAGAA        CATCTGTCGTATTTCAACT TAAAA-5'
	                                                            TATTTGACGTTTTCCGACT
	                                                                  Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 001-234812284
Forward Primer: 5'-GCCTGCCTGCTGAAGACATAAG-3' Tm: 59.258
Reverse Primer: 5'-CGCCTGACTTTATTGAATGCAGCC-3' Tm: 59.038
Expected PCR Product Length: 486
Expected CelI Cleavage Product Sizes: 350, 136
In Silico PCR
Genome Browser

Rank: 16	Homology Score: 2	RVD Match Score: 2.564	Left Half-Site RVD Score: 6.941	Right Half-Site RVD Score: 5.842
	Genomic Region: Intergenic	Closest Gene: LARGE
Match Type: R30R
	          Right Nuclease
	         TCAGCCTTTTGCAGTTTAT
	5'-ATTTC TCATCCCTTTATTGTGTAT ATTTGAGGTTTACAACATGATGTTATAAGT ATAGATAGTAAAATGCTTA CTACA-3'
	3'-TAAAG AGTAGGGAAATAACACATA TAAACTCCAAATGTTGTACTACAATATTCA TATCTATCATTTTACGAAT GATGT-5'
	                                                            TATTTGACGTTTTCCGACT
	                                                                  Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 022-034808553
Forward Primer: 5'-CATGAGGAAGTGTCAGACAAACCC-3' Tm: 59.038
Reverse Primer: 5'-GCGGCAGAGGATCAGGAAAG-3' Tm: 59.508
Expected PCR Product Length: 669
Expected CelI Cleavage Product Sizes: 408, 261
In Silico PCR
Genome Browser

Rank: 17	Homology Score: 5	RVD Match Score: 2.567	Left Half-Site RVD Score: 7.503	Right Half-Site RVD Score: 5.573
	Genomic Region: Intergenic	Closest Gene: C3orf80
Match Type: R27L
	          Right Nuclease
	         TCAGCCTTTTGCAGTTTAT
	5'-AGCTT CCAGCCATTTGAAATGTAA  TGATTACTCCTTAGATTGTTTATTTTC   TTAGCATGTTCTTCACAAA CCTAA-3'
	3'-TCGAA GGTCGGTAAACTTTACATT  ACTAATGAGGAATCTAACAAATAAAAG   AATCGTACAAGAAGTGTTT GGATT-5'
	                                                            GGTCGTACAACGGGTGTTT
	                                                                   Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 003-159856803
Forward Primer: 5'-CCTTGGCGACAGAGCAAGAC-3' Tm: 59.508
Reverse Primer: 5'-CACGCACACACACACACGATAAG-3' Tm: 59.148
Expected PCR Product Length: 427
Expected CelI Cleavage Product Sizes: 253, 174
In Silico PCR
Genome Browser

Rank: 18	Homology Score: 5	RVD Match Score: 2.567	Left Half-Site RVD Score: 6.241	Right Half-Site RVD Score: 6.56
	Genomic Region: Intron	Closest Gene: SLC39A11
Match Type: R12R
	          Right Nuclease
	         TCAGCCTTTTGCAGTTTAT
	5'-CAAAT CCAGCCTTCTGTTCCTTAT          TAGGCACTTTTA          GTAACAGGCAAAAGGTTGA CATGG-3'
	3'-GTTTA GGTCGGAAGACAAGGAATA          ATCCGTGAAAAT          CATTGTCCGTTTTCCAACT GTACC-5'
	                                                            TATTTGACGTTTTCCGACT
	                                                                  Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 017-070934484
Forward Primer: 5'-AAAGCCCAGTTCCACATCTTCCTC-3' Tm: 59.038
Reverse Primer: 5'-GGTAGCAGCAGCCTTCATTAGGA-3' Tm: 59.148
Expected PCR Product Length: 376
Expected CelI Cleavage Product Sizes: 126, 250
In Silico PCR
Genome Browser

Rank: 19	Homology Score: 5	RVD Match Score: 2.569	Left Half-Site RVD Score: 8.063	Right Half-Site RVD Score: 4.943
	Genomic Region: Intergenic	Closest Gene: PVRL3-AS1
Match Type: R25R
	          Right Nuclease
	         TCAGCCTTTTGCAGTTTAT
	5'-GGTTG TAAGACTTCTACATTTTAC   TTTAAATGATAAAATATTAACTCAA    ATAAACAGTGAAATGTTGA GTAGA-3'
	3'-CCAAC ATTCTGAAGATGTAAAATG   AAATTTACTATTTTATAATTGAGTT    TATTTGTCACTTTACAACT CATCT-5'
	                                                            TATTTGACGTTTTCCGACT
	                                                                  Right Nuclease
Warning! Primer pair was generated using suboptimal parameters. 
Primer Index: 003-110537278
Forward Primer: 5'-GTGGGTCTCTCTTCACTTTATTGATTGTTTCC-3' Tm: 59.848
Reverse Primer: 5'-GGTAAGAAGAGCGAAACTCCGTC-3' Tm: 59.148
Expected PCR Product Length: 598
Expected CelI Cleavage Product Sizes: 237, 361
In Silico PCR
Genome Browser

Rank: 20	Homology Score: 13	RVD Match Score: 2.57	Left Half-Site RVD Score: 5.706	Right Half-Site RVD Score: 7.391
	Genomic Region: Intergenic	Closest Gene: AOAH
Match Type: L24R
	           Left Nuclease
	         TTTGTGGGCAACATGCTGG
	5'-GTCCT CTTGTAGACAACATGCATG    CCTCCCTGCTCCCATTTGCTATCT    TTAATCTGCTAAAGGCTAA AAACC-3'
	3'-CAGGA GAACATCTGTTGTACGTAC    GGAGGGACGAGGGTAAACGATAGA    AATTAGACGATTTCCGATT TTTGG-5'
	                                                            TATTTGACGTTTTCCGACT
	                                                                  Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 007-036783522
Forward Primer: 5'-GGTTGTTTCTCTTCACTCACTGGC-3' Tm: 59.038
Reverse Primer: 5'-GCCCAACAATGCTGAACACAGATG-3' Tm: 59.038
Expected PCR Product Length: 352
Expected CelI Cleavage Product Sizes: 129, 223
In Silico PCR
Genome Browser

Rank: 21	Homology Score: 10	RVD Match Score: 2.573	Left Half-Site RVD Score: 7.191	Right Half-Site RVD Score: 6.156
	Genomic Region: Intron	Closest Gene: RIMS2
Match Type: L24L
	           Left Nuclease
	         TTTGTGGGCAACATGCTGG
	5'-ATGGA TATCTCTTCAACATACTGG    AGTCTTTAGGGTTTACTGTAGATA    AGATCATGTTGTCCACAAA CAGAG-3'
	3'-TACCT ATAGAGAAGTTGTATGACC    TCAGAAATCCCAAATGACATCTAT    TCTAGTACAACAGGTGTTT GTCTC-5'
	                                                            GGTCGTACAACGGGTGTTT
	                                                                   Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 008-104699899
Forward Primer: 5'-CATACCCCAGTCTCTGGTGATC-3' Tm: 59.258
Reverse Primer: 5'-CTCAACGGTGAAAAGTTGGAAACTTTCCTC-3' Tm: 60.058
Expected PCR Product Length: 542
Expected CelI Cleavage Product Sizes: 345, 197
In Silico PCR
Genome Browser

Rank: 22	Homology Score: 5	RVD Match Score: 2.574	Left Half-Site RVD Score: 7.638	Right Half-Site RVD Score: 5.564
	Genomic Region: Intergenic	Closest Gene: PXDNL
Match Type: L20R
	           Left Nuclease
	         TTTGTGGGCAACATGCTGG
	5'-GGCTA TGTGTAGGAAACATACTTT      AGATTTAAAGAAACAAATAG      ATTAATGGTAAAAGGATGA AGAAA-3'
	3'-CCGAT ACACATCCTTTGTATGAAA      TCTAAATTTCTTTGTTTATC      TAATTACCATTTTCCTACT TCTTT-5'
	                                                            TATTTGACGTTTTCCGACT
	                                                                  Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 008-051977566
Forward Primer: 5'-CAGAGATCGCACCATTGCATTCC-3' Tm: 59.148
Reverse Primer: 5'-GTCTAAGAATCTGTGGGCAAATGTATGGTC-3' Tm: 60.058
Expected PCR Product Length: 341
Expected CelI Cleavage Product Sizes: 126, 215
In Silico PCR
Genome Browser

Rank: 23	Homology Score: 8	RVD Match Score: 2.578	Left Half-Site RVD Score: 6.363	Right Half-Site RVD Score: 6.777
	Genomic Region: Exon	Closest Gene: PPM1E
Match Type: R18L
	          Right Nuclease
	         TCAGCCTTTTGCAGTTTAT
	5'-AACTG TCTGCTTTTTGCTATTTCT       GCAGTTTCAAGTTAGTTA       GAAGCATGTTGTCAACTAA AGACA-3'
	3'-TTGAC AGACGAAAAACGATAAAGA       CGTCAAAGTTCAATCAAT       CTTCGTACAACAGTTGATT TCTGT-5'
	                                                            GGTCGTACAACGGGTGTTT
	                                                                   Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 017-057059452
Forward Primer: 5'-GTCTAAAGTGAGCCCAGAGGCC-3' Tm: 61.288
Reverse Primer: 5'-CTGCTGTTGACTAGGGGAGG-3' Tm: 59.508
Expected PCR Product Length: 325
Expected CelI Cleavage Product Sizes: 200, 125
In Silico PCR
Genome Browser

Rank: 24	Homology Score: 2	RVD Match Score: 2.581	Left Half-Site RVD Score: 6.488	Right Half-Site RVD Score: 6.679
	Genomic Region: Intron	Closest Gene: HS3ST4
Match Type: L13R
	           Left Nuclease
	         TTTGTGGGCAACATGCTGG
	5'-TTGGG CTTGCATGCAACATACAGG         GCAGCTTGTTGTT          ATAAAAGGGAAGTGGTTGA CTTCT-3'
	3'-AACCC GAACGTACGTTGTATGTCC         CGTCGAACAACAA          TATTTTCCCTTCACCAACT GAAGA-5'
	                                                            TATTTGACGTTTTCCGACT
	                                                                  Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 016-025792276
Forward Primer: 5'-AGTAGAAGGCTGGCCCCACA-3' Tm: 59.508
Reverse Primer: 5'-CTCCAAAGAAACCCATCAGCAGTC-3' Tm: 59.038
Expected PCR Product Length: 357
Expected CelI Cleavage Product Sizes: 138, 219
In Silico PCR
Genome Browser

Rank: 25	Homology Score: 5	RVD Match Score: 2.583	Left Half-Site RVD Score: 6.481	Right Half-Site RVD Score: 6.952
	Genomic Region: Intergenic	Closest Gene: GRIN2A
Match Type: L18L
	           Left Nuclease
	         TTTGTGGGCAACATGCTGG
	5'-ATCCT TTTTTGTACAACATTCTAA       AAATCCTCATAGCCACTG       CCAGGAGGCTGCTCAGAAA GCTTC-3'
	3'-TAGGA AAAAACATGTTGTAAGATT       TTTAGGAGTATCGGTGAC       GGTCCTCCGACGAGTCTTT CGAAG-5'
	                                                            GGTCGTACAACGGGTGTTT
	                                                                   Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 016-009691841
Forward Primer: 5'-GATTGGAAGTCCCAGCACATGACA-3' Tm: 59.038
Reverse Primer: 5'-CCTTTGTTGCATAAGCCGTCACTG-3' Tm: 59.038
Expected PCR Product Length: 331
Expected CelI Cleavage Product Sizes: 204, 127
In Silico PCR
Genome Browser

Rank: 26	Homology Score: 17	RVD Match Score: 2.584	Left Half-Site RVD Score: 6.068	Right Half-Site RVD Score: 7.142
	Genomic Region: Intergenic	Closest Gene: FAM75C2
Match Type: L20R
	           Left Nuclease
	         TTTGTGGGCAACATGCTGG
	5'-TATGA TTTTTGGGCAACATAGTGG      GACTCTGTCTACAAAACTAA      ATAAATAGAAAGAGGCCCA GTGCG-3'
	3'-ATACT AAAAACCCGTTGTATCACC      CTGAGACAGATGTTTTGATT      TATTTATCTTTCTCCGGGT CACGC-5'
	                                                            TATTTGACGTTTTCCGACT
	                                                                  Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 009-090818650
Forward Primer: 5'-CTGCAAGGACTCAAAAAATTTGACCCCTAC-3' Tm: 60.058
Reverse Primer: 5'-TCACTATATGGGCCAGGCTGATC-3' Tm: 59.148
Expected PCR Product Length: 350
Expected CelI Cleavage Product Sizes: 225, 125
In Silico PCR
Genome Browser

Rank: 27	Homology Score: 10	RVD Match Score: 2.586	Left Half-Site RVD Score: 7.497	Right Half-Site RVD Score: 5.564
	Genomic Region: Intergenic	Closest Gene: SFT2D2
Match Type: R10R
	          Right Nuclease
	         TCAGCCTTTTGCAGTTTAT
	5'-CAAAT TAAGCCTTTTCCCTTACAT           GCATTGTTTC           ATAAAATGTAAATGGTTGA AGGGT-3'
	3'-GTTTA ATTCGGAAAAGGGAATGTA           CGTAACAAAG           TATTTTACATTTACCAACT TCCCA-5'
	                                                            TATTTGACGTTTTCCGACT
	                                                                  Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 001-168187120
Forward Primer: 5'-CCTACAGGTGTCCTCTTGTGG-3' Tm: 59.378
Reverse Primer: 5'-GCGCCCCTTCAGAGAAATGAG-3' Tm: 59.378
Expected PCR Product Length: 546
Expected CelI Cleavage Product Sizes: 186, 360
In Silico PCR
Genome Browser

Rank: 28	Homology Score: 5	RVD Match Score: 2.588	Left Half-Site RVD Score: 7.229	Right Half-Site RVD Score: 6.029
	Genomic Region: Intron	Closest Gene: LINC00536
Match Type: R14L
	          Right Nuclease
	         TCAGCCTTTTGCAGTTTAT
	5'-CAAAA TCAACCTTCTGAAGTTCCT         GTGCCTTTTAAAAA         TTAGTGTGTTTCTCACAAA ACTGA-3'
	3'-GTTTT AGTTGGAAGACTTCAAGGA         CACGGAAAATTTTT         AATCACACAAAGAGTGTTT TGACT-5'
	                                                            GGTCGTACAACGGGTGTTT
	                                                                   Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 008-117246045
Forward Primer: 5'-TTCTCTGCCACGAGTGTCTTGC-3' Tm: 59.258
Reverse Primer: 5'-TTGTCTCTGTCTTTGCCCTGCC-3' Tm: 59.258
Expected PCR Product Length: 327
Expected CelI Cleavage Product Sizes: 126, 201
In Silico PCR
Genome Browser

Rank: 29	Homology Score: 5	RVD Match Score: 2.588	Left Half-Site RVD Score: 5.989	Right Half-Site RVD Score: 7.299
	Genomic Region: Intergenic	Closest Gene: EPHA7
Match Type: R11L
	          Right Nuclease
	         TCAGCCTTTTGCAGTTTAT
	5'-AAATT TCAGCTCTTTACCATTTAT          TACTTAGTTTA           CAAGAATGTTTCATATAAA TAGTA-3'
	3'-TTTAA AGTCGAGAAATGGTAAATA          ATGAATCAAAT           GTTCTTACAAAGTATATTT ATCAT-5'
	                                                            GGTCGTACAACGGGTGTTT
	                                                                   Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 006-093167839
Forward Primer: 5'-CTCACCTCAGATTCTCTTGGGAGT-3' Tm: 59.038
Reverse Primer: 5'-CACTAAGGAATTTCCCCTCTATCAAAGGC-3' Tm: 60.178
Expected PCR Product Length: 511
Expected CelI Cleavage Product Sizes: 159, 352
In Silico PCR
Genome Browser

Rank: 30	Homology Score: 2	RVD Match Score: 2.595	Left Half-Site RVD Score: 7.021	Right Half-Site RVD Score: 6.54
	Genomic Region: Intergenic	Closest Gene: RAB11FIP2
Match Type: L24L
	           Left Nuclease
	         TTTGTGGGCAACATGCTGG
	5'-AACTC TTTGCCGGCAAGATCCTAA    AAAAGGAATTGTCCTATTCAGAAC    TCAGGGTGTTGTTCCCAAA TTCCT-3'
	3'-TTGAG AAACGGCCGTTCTAGGATT    TTTTCCTTAACAGGATAAGTCTTG    AGTCCCACAACAAGGGTTT AAGGA-5'
	                                                            GGTCGTACAACGGGTGTTT
	                                                                   Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 010-119687443
Forward Primer: 5'-ATGGGCTGTACACACCCCTG-3' Tm: 59.508
Reverse Primer: 5'-GTTGACAAAAGGAGTGGCGTATGC-3' Tm: 59.038
Expected PCR Product Length: 388
Expected CelI Cleavage Product Sizes: 234, 154
In Silico PCR
Genome Browser

Rank: 31	Homology Score: 2	RVD Match Score: 2.606	Left Half-Site RVD Score: 6.014	Right Half-Site RVD Score: 7.462
	Genomic Region: Intron	Closest Gene: SEMA3C
Match Type: R10L
	          Right Nuclease
	         TCAGCCTTTTGCAGTTTAT
	5'-CTCAC TCACCACTTTTCTATTTAT           TTTGTCCCAG           ACACCATTTTGTTCAGAAA ACAAA-3'
	3'-GAGTG AGTGGTGAAAAGATAAATA           AAACAGGGTC           TGTGGTAAAACAAGTCTTT TGTTT-5'
	                                                            GGTCGTACAACGGGTGTTT
	                                                                   Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 007-080418831
Forward Primer: 5'-GCTCCTCCTGGACACTAGAAAG-3' Tm: 59.258
Reverse Primer: 5'-CTTTTGGGAGGGCAGATCAGATG-3' Tm: 59.148
Expected PCR Product Length: 469
Expected CelI Cleavage Product Sizes: 323, 146
In Silico PCR
Genome Browser

Rank: 32	Homology Score: 5	RVD Match Score: 2.606	Left Half-Site RVD Score: 8.308	Right Half-Site RVD Score: 5.078
	Genomic Region: Intron	Closest Gene: MAGI1
Match Type: R29R
	          Right Nuclease
	         TCAGCCTTTTGCAGTTTAT
	5'-CTAAG CCATACTTTTGGAGATCAT TTGAAATATAGCACATAGGTATATGAAAA  ATAAACAGGTAATGGCTGG GTGTG-3'
	3'-GATTC GGTATGAAAACCTCTAGTA AACTTTATATCGTGTATCCATATACTTTT  TATTTGTCCATTACCGACC CACAC-5'
	                                                            TATTTGACGTTTTCCGACT
	                                                                  Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 003-065965730
Forward Primer: 5'-GGGTGGGACAAGTGAACAGTG-3' Tm: 59.378
Reverse Primer: 5'-TGTTGGCCAGGCTGGTCTTG-3' Tm: 59.508
Expected PCR Product Length: 326
Expected CelI Cleavage Product Sizes: 200, 126
In Silico PCR
Genome Browser

Rank: 33	Homology Score: 5	RVD Match Score: 2.606	Left Half-Site RVD Score: 5.544	Right Half-Site RVD Score: 7.97
	Genomic Region: Intergenic	Closest Gene: ABCD2
Match Type: L12R
	           Left Nuclease
	         TTTGTGGGCAACATGCTGG
	5'-TTAGG TTTCAAGACAACATGCTAA          ATACATAGGACC          ATCTACTGGCAAAGGATGA TATAG-3'
	3'-AATCC AAAGTTCTGTTGTACGATT          TATGTATCCTGG          TAGATGACCGTTTCCTACT ATATC-5'
	                                                            TATTTGACGTTTTCCGACT
	                                                                  Right Nuclease
Warning! Primer pair was generated using suboptimal parameters. 
Primer Index: 012-039942796
Forward Primer: 5'-GAACTTTCCTGCTATGTTTATCTGAGTTTTGTGG-3' Tm: 59.668
Reverse Primer: 5'-GTCCTTGTCAGAGCCCTCATG-3' Tm: 59.378
Expected PCR Product Length: 438
Expected CelI Cleavage Product Sizes: 139, 299
In Silico PCR
Genome Browser

Rank: 34	Homology Score: 5	RVD Match Score: 2.61	Left Half-Site RVD Score: 7.605	Right Half-Site RVD Score: 5.904
	Genomic Region: Intergenic	Closest Gene: SF3B1
Match Type: R20L
	          Right Nuclease
	         TCAGCCTTTTGCAGTTTAT
	5'-AAAGT TCCGCATTTTACAAGTCAT      GTTCAAAAAACACACACAAA      TTAGCATTTTGTACACAAA TGTTT-3'
	3'-TTTCA AGGCGTAAAATGTTCAGTA      CAAGTTTTTTGTGTGTGTTT      AATCGTAAAACATGTGTTT ACAAA-5'
	                                                            GGTCGTACAACGGGTGTTT
	                                                                   Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 002-198256316
Forward Primer: 5'-CAGACCTGTCAAGTGTTGGACAAG-3' Tm: 59.038
Reverse Primer: 5'-GCCAGTAGTGACCAAGAACACAG-3' Tm: 59.148
Expected PCR Product Length: 531
Expected CelI Cleavage Product Sizes: 374, 157
In Silico PCR
Genome Browser

Rank: 35	Homology Score: 10	RVD Match Score: 2.615	Left Half-Site RVD Score: 6.155	Right Half-Site RVD Score: 7.14
	Genomic Region: Intergenic	Closest Gene: KCTD3
Match Type: R27R
	          Right Nuclease
	         TCAGCCTTTTGCAGTTTAT
	5'-CACTC CCACCCTCTTGCAGTTTAG  ACACAACTGACCAGCATTAACGTTAAA   ATAGAGATCATAAGACTGA TAGAA-3'
	3'-GTGAG GGTGGGAGAACGTCAAATC  TGTGTTGACTGGTCGTAATTGCAATTT   TATCTCTAGTATTCTGACT ATCTT-5'
	                                                            TATTTGACGTTTTCCGACT
	                                                                  Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 001-215692850
Forward Primer: 5'-CTAAATGGACCTCCTCTTGGCC-3' Tm: 59.258
Reverse Primer: 5'-CCTGACCTCTGGTGATCTGC-3' Tm: 59.508
Expected PCR Product Length: 348
Expected CelI Cleavage Product Sizes: 136, 212
In Silico PCR
Genome Browser

Rank: 36	Homology Score: 5	RVD Match Score: 2.616	Left Half-Site RVD Score: 5.783	Right Half-Site RVD Score: 7.843
	Genomic Region: Intergenic	Closest Gene: C5orf38
Match Type: R22L
	          Right Nuclease
	         TCAGCCTTTTGCAGTTTAT
	5'-TTACA CCAGCTTTTTTCTGCTTAT     TTGTTGCATGGTATATATTTTC     CCATCCTTTTGCTTTCAAA TGTAA-3'
	3'-AATGT GGTCGAAAAAAGACGAATA     AACAACGTACCATATATAAAAG     GGTAGGAAAACGAAAGTTT ACATT-5'
	                                                            GGTCGTACAACGGGTGTTT
	                                                                   Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 005-002941667
Forward Primer: 5'-GCAATGATCTTGAGCAGCAGGAG-3' Tm: 59.148
Reverse Primer: 5'-GCACGTTCTGCACATGTATCCC-3' Tm: 59.258
Expected PCR Product Length: 480
Expected CelI Cleavage Product Sizes: 148, 332
In Silico PCR
Genome Browser

Rank: 37	Homology Score: 2	RVD Match Score: 2.622	Left Half-Site RVD Score: 6.951	Right Half-Site RVD Score: 6.642
	Genomic Region: Intron	Closest Gene: ZNF326
Match Type: L18R
	           Left Nuclease
	         TTTGTGGGCAACATGCTGG
	5'-ATTTT TTCTTGGACAATATGCTAA       TATTTATTGATTTCACAA       ATTTACAGCATATGGGTGA TTTTG-3'
	3'-TAAAA AAGAACCTGTTATACGATT       ATAAATAACTAAAGTGTT       TAAATGTCGTATACCCACT AAAAC-5'
	                                                            TATTTGACGTTTTCCGACT
	                                                                  Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 001-090475333
Forward Primer: 5'-GTGGTAACATTTGGACAGGCACATATGTTG-3' Tm: 60.058
Reverse Primer: 5'-CCACTGGGCTTATTAAATGGCTGC-3' Tm: 59.038
Expected PCR Product Length: 449
Expected CelI Cleavage Product Sizes: 299, 150
In Silico PCR
Genome Browser

Rank: 38	Homology Score: 10	RVD Match Score: 2.623	Left Half-Site RVD Score: 6.759	Right Half-Site RVD Score: 6.598
	Genomic Region: Intergenic	Closest Gene: OR6M1
Match Type: R30R
	          Right Nuclease
	         TCAGCCTTTTGCAGTTTAT
	5'-TTTTG TCAGCCTTAAGATCTCTAT TTTAACATTAATGCTGATCAGTTATTGTAC CTAAACTCCAAAAGGGTGG AGGTA-3'
	3'-AAAAC AGTCGGAATTCTAGAGATA AAATTGTAATTACGACTAGTCAATAACATG GATTTGAGGTTTTCCCACC TCCAT-5'
	                                                            TATTTGACGTTTTCCGACT
	                                                                  Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 011-123693390
Forward Primer: 5'-GGTTATTGGTGGATTCGGAGAGTC-3' Tm: 59.038
Reverse Primer: 5'-GAATGGACTCCCTCTTGGCC-3' Tm: 59.508
Expected PCR Product Length: 380
Expected CelI Cleavage Product Sizes: 248, 132
In Silico PCR
Genome Browser

Rank: 39	Homology Score: 5	RVD Match Score: 2.624	Left Half-Site RVD Score: 7.653	Right Half-Site RVD Score: 6.02
	Genomic Region: Intron	Closest Gene: ABCC1
Match Type: L29R
	           Left Nuclease
	         TTTGTGGGCAACATGCTGG
	5'-CATGC CTTGAGGGGACCATGCTTA TGAAGTGCCTGGCACACAGTGGATGGACA  ATAAATGGCAACAGTTTGA TTGTG-3'
	3'-GTACG GAACTCCCCTGGTACGAAT ACTTCACGGACCGTGTGTCACCTACCTGT  TATTTACCGTTGTCAAACT AACAC-5'
	                                                            TATTTGACGTTTTCCGACT
	                                                                  Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 016-016192954
Forward Primer: 5'-ACAGCCTGAGTCCCCAGATC-3' Tm: 59.508
Reverse Primer: 5'-AGGAGGGATGGGTCCAGTCT-3' Tm: 59.508
Expected PCR Product Length: 326
Expected CelI Cleavage Product Sizes: 125, 201
In Silico PCR
Genome Browser

Rank: 40	Homology Score: 13	RVD Match Score: 2.624	Left Half-Site RVD Score: 6.843	Right Half-Site RVD Score: 6.767
	Genomic Region: Intergenic	Closest Gene: KDM5D
Match Type: R25L
	          Right Nuclease
	         TCAGCCTTTTGCAGTTTAT
	5'-TATGG TAAGCACTTTGTTGTTTAT   GGGCTTCTTTTCCACCTATCAATTC    CCAGTTGGTTGCCCCCAAA ATGAA-3'
	3'-ATACC ATTCGTGAAACAACAAATA   CCCGAAGAAAAGGTGGATAGTTAAG    GGTCAACCAACGGGGGTTT TACTT-5'
	                                                            GGTCGTACAACGGGTGTTT
	                                                                   Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 00Y-022149362
Forward Primer: 5'-CATGACTGACACCCTCCACG-3' Tm: 59.508
Reverse Primer: 5'-AGCAAAGCAGGTGGCACAGC-3' Tm: 59.508
Expected PCR Product Length: 330
Expected CelI Cleavage Product Sizes: 203, 127
In Silico PCR
Genome Browser

Rank: 41	Homology Score: 8	RVD Match Score: 2.626	Left Half-Site RVD Score: 6.358	Right Half-Site RVD Score: 7.038
	Genomic Region: Intergenic	Closest Gene: ACTRT1
Match Type: R30R
	          Right Nuclease
	         TCAGCCTTTTGCAGTTTAT
	5'-TTCTA TCAGTCTTATGATCTTTAT TTTAACATTAATGCTGAGCAGTTGTTGTGT CTGAACTGCAAAAGGTAGG GGGTA-3'
	3'-AAGAT AGTCAGAATACTAGAAATA AAATTGTAATTACGACTCGTCAACAACACA GACTTGACGTTTTCCATCC CCCAT-5'
	                                                            TATTTGACGTTTTCCGACT
	                                                                  Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 00X-127154714
Forward Primer: 5'-GGAAGGGAAGCAGAAGTTACAGAC-3' Tm: 59.038
Reverse Primer: 5'-CTTTTGCACAATCCCCTCTTTGCC-3' Tm: 59.038
Expected PCR Product Length: 498
Expected CelI Cleavage Product Sizes: 366, 132
In Silico PCR
Genome Browser

Rank: 42	Homology Score: 5	RVD Match Score: 2.629	Left Half-Site RVD Score: 7.053	Right Half-Site RVD Score: 6.373
	Genomic Region: Intron	Closest Gene: PDE1C
Match Type: R13R
	          Right Nuclease
	         TCAGCCTTTTGCAGTTTAT
	5'-CTCTC TCTGCCTTTTTCTCTCTCT         TTCCCCCCACAAC          ACACACATCAAAAGGTTGA GCTTG-3'
	3'-GAGAG AGACGGAAAAAGAGAGAGA         AAGGGGGGTGTTG          TGTGTGTAGTTTTCCAACT CGAAC-5'
	                                                            TATTTGACGTTTTCCGACT
	                                                                  Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 007-032193295
Forward Primer: 5'-GATAGAGCCCACGTAGTGTAGTC-3' Tm: 59.148
Reverse Primer: 5'-CTGCACTGGACTCCCAAAATACTG-3' Tm: 59.038
Expected PCR Product Length: 345
Expected CelI Cleavage Product Sizes: 210, 135
In Silico PCR
Genome Browser

Rank: 43	Homology Score: 5	RVD Match Score: 2.631	Left Half-Site RVD Score: 5.977	Right Half-Site RVD Score: 7.503
	Genomic Region: Intergenic	Closest Gene: GUCY1A2
Match Type: R20R
	          Right Nuclease
	         TCAGCCTTTTGCAGTTTAT
	5'-CTTCC TCACCATCATGCTGTTTAT      ATTAGGCTTTAAAAATATTA      CTTAATGGCAATATGCTGA AACCT-3'
	3'-GAAGG AGTGGTAGTACGACAAATA      TAATCCGAAATTTTTATAAT      GAATTACCGTTATACGACT TTGGA-5'
	                                                            TATTTGACGTTTTCCGACT
	                                                                  Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 011-106518241
Forward Primer: 5'-CCAGTTTGACCCATACTACTGAGTCTCT-3' Tm: 60.298
Reverse Primer: 5'-GGGATGACACATAGTGCCATCTG-3' Tm: 59.148
Expected PCR Product Length: 334
Expected CelI Cleavage Product Sizes: 126, 208
In Silico PCR
Genome Browser

Rank: 44	Homology Score: 5	RVD Match Score: 2.635	Left Half-Site RVD Score: 8.3	Right Half-Site RVD Score: 5.787
	Genomic Region: Intergenic	Closest Gene: EXOC6B
Match Type: L10L
	           Left Nuclease
	         TTTGTGGGCAACATGCTGG
	5'-AGATC TTTATGGGCTTCATAAAGG           ATGAAGAAAT           TCTGTATGTTGCCAATAAA ATATA-3'
	3'-TCTAG AAATACCCGAAGTATTTCC           TACTTCTTTA           AGACATACAACGGTTATTT TATAT-5'
	                                                            GGTCGTACAACGGGTGTTT
	                                                                   Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 002-072399256
Forward Primer: 5'-CTCCCAAAGTGCTGGGATTACAGG-3' Tm: 60.908
Reverse Primer: 5'-CCCTAATCCCATTTCCCACATCTG-3' Tm: 59.038
Expected PCR Product Length: 457
Expected CelI Cleavage Product Sizes: 266, 191
In Silico PCR
Genome Browser

Rank: 45	Homology Score: 2	RVD Match Score: 2.637	Left Half-Site RVD Score: 6.507	Right Half-Site RVD Score: 6.995
	Genomic Region: Intergenic	Closest Gene: MANEA
Match Type: R25R
	          Right Nuclease
	         TCAGCCTTTTGCAGTTTAT
	5'-GAGAT CCAGCCTTTCAAAAATTAT   GAAAGTTAGAAACAGAAAGTAAGTC    ATAAAAAGAAATATGATGA AAGAA-3'
	3'-CTCTA GGTCGGAAAGTTTTTAATA   CTTTCAATCTTTGTCTTTCATTCAG    TATTTTTCTTTATACTACT TTCTT-5'
	                                                            TATTTGACGTTTTCCGACT
	                                                                  Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 006-095270874
Forward Primer: 5'-CCCAAAGAGGACCTTTACTCTCAC-3' Tm: 59.038
Reverse Primer: 5'-CCCTAAGCATAGTGGACAAGGC-3' Tm: 59.258
Expected PCR Product Length: 334
Expected CelI Cleavage Product Sizes: 125, 209
In Silico PCR
Genome Browser

Rank: 46	Homology Score: 13	RVD Match Score: 2.637	Left Half-Site RVD Score: 8.507	Right Half-Site RVD Score: 5.194
	Genomic Region: Intron	Closest Gene: MAP3K5
Match Type: R27R
	          Right Nuclease
	         TCAGCCTTTTGCAGTTTAT
	5'-ATACC TCCTCCCTTTGGAGTTTAG  GCACAACTGACCAGCATTACCATTAAA   ATAAAGATCATAAGGCTGA CAAAA-3'
	3'-TATGG AGGAGGGAAACCTCAAATC  CGTGTTGACTGGTCGTAATGGTAATTT   TATTTCTAGTATTCCGACT GTTTT-5'
	                                                            TATTTGACGTTTTCCGACT
	                                                                  Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 006-136890071
Forward Primer: 5'-GAAGAACCACCTACCCCCAG-3' Tm: 59.508
Reverse Primer: 5'-CTACAGAATGCAGATTTCCCCCAC-3' Tm: 59.038
Expected PCR Product Length: 348
Expected CelI Cleavage Product Sizes: 135, 213
In Silico PCR
Genome Browser

Rank: 47	Homology Score: 2	RVD Match Score: 2.637	Left Half-Site RVD Score: 6.426	Right Half-Site RVD Score: 7.343
	Genomic Region: Intron	Closest Gene: FAM160A1
Match Type: R30L
	          Right Nuclease
	         TCAGCCTTTTGCAGTTTAT
	5'-TGTAC TCAGCATAATACTGATTAT ATTATATAGTTTCAAGTTTTCTTTTATTTT AAAGCATGTTTTCTTCAAA TGAAG-3'
	3'-ACATG AGTCGTATTATGACTAATA TAATATATCAAAGTTCAAAAGAAAATAAAA TTTCGTACAAAAGAAGTTT ACTTC-5'
	                                                            GGTCGTACAACGGGTGTTT
	                                                                   Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 004-152360042
Forward Primer: 5'-CACCTCAGCCTCCGGAATTTTG-3' Tm: 59.258
Reverse Primer: 5'-ACTCCTTTAGTCCCCACAACAACC-3' Tm: 59.038
Expected PCR Product Length: 411
Expected CelI Cleavage Product Sizes: 168, 243
In Silico PCR
Genome Browser

Rank: 48	Homology Score: 2	RVD Match Score: 2.638	Left Half-Site RVD Score: 6.987	Right Half-Site RVD Score: 6.528
	Genomic Region: Intron	Closest Gene: PCDH11Y
Match Type: R20R
	          Right Nuclease
	         TCAGCCTTTTGCAGTTTAT
	5'-TGTTC TCAGCCTTTCTCTGTCACT      AGGCTCAGTAGGAAGGAAGG      ATAAATGGCAAATGTTGGA TGGGA-3'
	3'-ACAAG AGTCGGAAAGAGACAGTGA      TCCGAGTCATCCTTCCTTCC      TATTTACCGTTTACAACCT ACCCT-5'
	                                                            TATTTGACGTTTTCCGACT
	                                                                  Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 00Y-005558821
Forward Primer: 5'-GCAGGCTTACTTGAAGTCCCAG-3' Tm: 59.258
Reverse Primer: 5'-CGTGCCTACTAAGAACTGCTCTC-3' Tm: 59.148
Expected PCR Product Length: 683
Expected CelI Cleavage Product Sizes: 253, 430
In Silico PCR
Genome Browser

Plate format Oligo Order Form
Well
Name
Sequence
5' mods
Internal
Notes
Primer Plate #1
A1
003-046414377F
GCACAGGGTGGAACAAGATGG
12212012SXJ93rlv_QUEUE_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
A2
003-046414377R
GTGACCGTCCTGGCTTTTAAAGCA
12212012SXJ93rlv_QUEUE_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
B1
003-046399009F
CATCCACAACATGCTGTCCACATC
12212012SXJ93rlv_QUEUE_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
B2
003-046399009R
CAAAGACCCACTCATTTGCAGCAG
12212012SXJ93rlv_QUEUE_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
C1
005-078100805F
TAGCAGGGACACAGCATCCG
12212012SXJ93rlv_QUEUE_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
C2
005-078100805R
CTCGTTGGCTGACTTATGCTCTG
12212012SXJ93rlv_QUEUE_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
D1
005-091821506F
AGGACTGGCTCCTTGTGGTG
12212012SXJ93rlv_QUEUE_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
D2
005-091821506R
GACCTGCTTCTCTGGTCTTTTCTG
12212012SXJ93rlv_QUEUE_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
E1
005-118490138F
GTCTAGATGGATCGAACTAGCCAC
12212012SXJ93rlv_QUEUE_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
E2
005-118490138R
GCACTGACTGCTCTCTAACTACG
12212012SXJ93rlv_QUEUE_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
F1
003-157066450F
AAGGAAGAGGGGGCTGCAGA
12212012SXJ93rlv_QUEUE_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
F2
003-157066450R
GCTGAGTCCTTGTCTGGTTTTGG
12212012SXJ93rlv_QUEUE_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
G1
002-175789857F
GACTCCCTCTTGGCCAACAG
12212012SXJ93rlv_QUEUE_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
G2
002-175789857R
CAGTTGCAAATTCCCCCACAAGG
12212012SXJ93rlv_QUEUE_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
H1
004-113138626F
GCCATCCAAATGTGGACAGTAGG
12212012SXJ93rlv_QUEUE_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
H2
004-113138626R
GTAGGGGAAACCAATTACAGTGCC
12212012SXJ93rlv_QUEUE_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
A3
002-039561318F
CCCTCTGCATGATCTGGTTGCATT
12212012SXJ93rlv_QUEUE_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
A4
002-039561318R
GTAGTATCAGTGGGTGGGTGC
12212012SXJ93rlv_QUEUE_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
B3
003-015921847F
GCTCTAGAACTCTAGACTGTGGG
12212012SXJ93rlv_QUEUE_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
B4
003-015921847R
CTCACGGCCAATCCTGTTTTATCC
12212012SXJ93rlv_QUEUE_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
C3
00X-109896865F
CCATTGTACAGAGTGGTAACTCCAGTG
12212012SXJ93rlv_QUEUE_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
C4
00X-109896865R
GTTAGGGGGGAGTCCCTTCT
12212012SXJ93rlv_QUEUE_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
D3
001-246821429F
CAGCCAGATTCAAGATCCTCTCC
12212012SXJ93rlv_QUEUE_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
D4
001-246821429R
GCTTCCCTGAATGTGGGTCTATAG
12212012SXJ93rlv_QUEUE_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
E3
007-036433669F
ATCCTTTTGCTTCAGCCACCCC
12212012SXJ93rlv_QUEUE_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
E4
007-036433669R
AGATCCCCTCTTGATGAAGGGGAC
12212012SXJ93rlv_QUEUE_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
F3
015-056932411F
CAAGCCATATTGAAGCCTGAGGCA
12212012SXJ93rlv_QUEUE_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
F4
015-056932411R
GCATTTGCTTCCATTAGCCAGGAG
12212012SXJ93rlv_QUEUE_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
G3
001-234812284F
GCCTGCCTGCTGAAGACATAAG
12212012SXJ93rlv_QUEUE_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
G4
001-234812284R
CGCCTGACTTTATTGAATGCAGCC
12212012SXJ93rlv_QUEUE_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
H3
022-034808553F
CATGAGGAAGTGTCAGACAAACCC
12212012SXJ93rlv_QUEUE_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
H4
022-034808553R
GCGGCAGAGGATCAGGAAAG
12212012SXJ93rlv_QUEUE_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
A5
003-159856803F
CCTTGGCGACAGAGCAAGAC
12212012SXJ93rlv_QUEUE_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
A6
003-159856803R
CACGCACACACACACACGATAAG
12212012SXJ93rlv_QUEUE_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
B5
017-070934484F
AAAGCCCAGTTCCACATCTTCCTC
12212012SXJ93rlv_QUEUE_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
B6
017-070934484R
GGTAGCAGCAGCCTTCATTAGGA
12212012SXJ93rlv_QUEUE_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
C5
003-110537278F
GTGGGTCTCTCTTCACTTTATTGATTGTTTCC
12212012SXJ93rlv_QUEUE_Ranking-RVDs_5TC	Warning! Primer pair was generated using suboptimal parameters. 
C6
003-110537278R
GGTAAGAAGAGCGAAACTCCGTC
12212012SXJ93rlv_QUEUE_Ranking-RVDs_5TC	Warning! Primer pair was generated using suboptimal parameters. 
D5
007-036783522F
GGTTGTTTCTCTTCACTCACTGGC
12212012SXJ93rlv_QUEUE_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
D6
007-036783522R
GCCCAACAATGCTGAACACAGATG
12212012SXJ93rlv_QUEUE_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
E5
008-104699899F
CATACCCCAGTCTCTGGTGATC
12212012SXJ93rlv_QUEUE_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
E6
008-104699899R
CTCAACGGTGAAAAGTTGGAAACTTTCCTC
12212012SXJ93rlv_QUEUE_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
F5
008-051977566F
CAGAGATCGCACCATTGCATTCC
12212012SXJ93rlv_QUEUE_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
F6
008-051977566R
GTCTAAGAATCTGTGGGCAAATGTATGGTC
12212012SXJ93rlv_QUEUE_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
G5
017-057059452F
GTCTAAAGTGAGCCCAGAGGCC
12212012SXJ93rlv_QUEUE_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
G6
017-057059452R
CTGCTGTTGACTAGGGGAGG
12212012SXJ93rlv_QUEUE_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
H5
016-025792276F
AGTAGAAGGCTGGCCCCACA
12212012SXJ93rlv_QUEUE_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
H6
016-025792276R
CTCCAAAGAAACCCATCAGCAGTC
12212012SXJ93rlv_QUEUE_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
A7
016-009691841F
GATTGGAAGTCCCAGCACATGACA
12212012SXJ93rlv_QUEUE_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
A8
016-009691841R
CCTTTGTTGCATAAGCCGTCACTG
12212012SXJ93rlv_QUEUE_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
B7
009-090818650F
CTGCAAGGACTCAAAAAATTTGACCCCTAC
12212012SXJ93rlv_QUEUE_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
B8
009-090818650R
TCACTATATGGGCCAGGCTGATC
12212012SXJ93rlv_QUEUE_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
C7
001-168187120F
CCTACAGGTGTCCTCTTGTGG
12212012SXJ93rlv_QUEUE_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
C8
001-168187120R
GCGCCCCTTCAGAGAAATGAG
12212012SXJ93rlv_QUEUE_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
D7
008-117246045F
TTCTCTGCCACGAGTGTCTTGC
12212012SXJ93rlv_QUEUE_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
D8
008-117246045R
TTGTCTCTGTCTTTGCCCTGCC
12212012SXJ93rlv_QUEUE_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
E7
006-093167839F
CTCACCTCAGATTCTCTTGGGAGT
12212012SXJ93rlv_QUEUE_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
E8
006-093167839R
CACTAAGGAATTTCCCCTCTATCAAAGGC
12212012SXJ93rlv_QUEUE_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
F7
010-119687443F
ATGGGCTGTACACACCCCTG
12212012SXJ93rlv_QUEUE_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
F8
010-119687443R
GTTGACAAAAGGAGTGGCGTATGC
12212012SXJ93rlv_QUEUE_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
G7
007-080418831F
GCTCCTCCTGGACACTAGAAAG
12212012SXJ93rlv_QUEUE_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
G8
007-080418831R
CTTTTGGGAGGGCAGATCAGATG
12212012SXJ93rlv_QUEUE_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
H7
003-065965730F
GGGTGGGACAAGTGAACAGTG
12212012SXJ93rlv_QUEUE_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
H8
003-065965730R
TGTTGGCCAGGCTGGTCTTG
12212012SXJ93rlv_QUEUE_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
A9
012-039942796F
GAACTTTCCTGCTATGTTTATCTGAGTTTTGTGG
12212012SXJ93rlv_QUEUE_Ranking-RVDs_5TC	Warning! Primer pair was generated using suboptimal parameters. 
A10
012-039942796R
GTCCTTGTCAGAGCCCTCATG
12212012SXJ93rlv_QUEUE_Ranking-RVDs_5TC	Warning! Primer pair was generated using suboptimal parameters. 
B9
002-198256316F
CAGACCTGTCAAGTGTTGGACAAG
12212012SXJ93rlv_QUEUE_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
B10
002-198256316R
GCCAGTAGTGACCAAGAACACAG
12212012SXJ93rlv_QUEUE_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
C9
001-215692850F
CTAAATGGACCTCCTCTTGGCC
12212012SXJ93rlv_QUEUE_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
C10
001-215692850R
CCTGACCTCTGGTGATCTGC
12212012SXJ93rlv_QUEUE_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
D9
005-002941667F
GCAATGATCTTGAGCAGCAGGAG
12212012SXJ93rlv_QUEUE_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
D10
005-002941667R
GCACGTTCTGCACATGTATCCC
12212012SXJ93rlv_QUEUE_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
E9
001-090475333F
GTGGTAACATTTGGACAGGCACATATGTTG
12212012SXJ93rlv_QUEUE_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
E10
001-090475333R
CCACTGGGCTTATTAAATGGCTGC
12212012SXJ93rlv_QUEUE_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
F9
011-123693390F
GGTTATTGGTGGATTCGGAGAGTC
12212012SXJ93rlv_QUEUE_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
F10
011-123693390R
GAATGGACTCCCTCTTGGCC
12212012SXJ93rlv_QUEUE_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
G9
016-016192954F
ACAGCCTGAGTCCCCAGATC
12212012SXJ93rlv_QUEUE_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
G10
016-016192954R
AGGAGGGATGGGTCCAGTCT
12212012SXJ93rlv_QUEUE_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
H9
00Y-022149362F
CATGACTGACACCCTCCACG
12212012SXJ93rlv_QUEUE_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
H10
00Y-022149362R
AGCAAAGCAGGTGGCACAGC
12212012SXJ93rlv_QUEUE_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
A11
00X-127154714F
GGAAGGGAAGCAGAAGTTACAGAC
12212012SXJ93rlv_QUEUE_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
A12
00X-127154714R
CTTTTGCACAATCCCCTCTTTGCC
12212012SXJ93rlv_QUEUE_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
B11
007-032193295F
GATAGAGCCCACGTAGTGTAGTC
12212012SXJ93rlv_QUEUE_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
B12
007-032193295R
CTGCACTGGACTCCCAAAATACTG
12212012SXJ93rlv_QUEUE_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
C11
011-106518241F
CCAGTTTGACCCATACTACTGAGTCTCT
12212012SXJ93rlv_QUEUE_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
C12
011-106518241R
GGGATGACACATAGTGCCATCTG
12212012SXJ93rlv_QUEUE_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
D11
002-072399256F
CTCCCAAAGTGCTGGGATTACAGG
12212012SXJ93rlv_QUEUE_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
D12
002-072399256R
CCCTAATCCCATTTCCCACATCTG
12212012SXJ93rlv_QUEUE_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
E11
006-095270874F
CCCAAAGAGGACCTTTACTCTCAC
12212012SXJ93rlv_QUEUE_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
E12
006-095270874R
CCCTAAGCATAGTGGACAAGGC
12212012SXJ93rlv_QUEUE_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
F11
006-136890071F
GAAGAACCACCTACCCCCAG
12212012SXJ93rlv_QUEUE_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
F12
006-136890071R
CTACAGAATGCAGATTTCCCCCAC
12212012SXJ93rlv_QUEUE_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
G11
004-152360042F
CACCTCAGCCTCCGGAATTTTG
12212012SXJ93rlv_QUEUE_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
G12
004-152360042R
ACTCCTTTAGTCCCCACAACAACC
12212012SXJ93rlv_QUEUE_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
H11
00Y-005558821F
GCAGGCTTACTTGAAGTCCCAG
12212012SXJ93rlv_QUEUE_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
H12
00Y-005558821R
CGTGCCTACTAAGAACTGCTCTC
12212012SXJ93rlv_QUEUE_Ranking-RVDs_5TC	Primer pair generated using optimal parameters.