PROGNOS Summary for 12212012SXJ93rlv_QUEUE_Ranking-RVDs_5T

The hg19 genome was searched for the TALEN pair targeting:
5'- TTTGTGGGCAACATGCTGG NN..NN ATAAACTGCAAAAGGCTGA -3'

Using these RVDs:
Left:  01NG02NG03NN04NG05NN06NN07NN08HD09NI10NI11HD12NI13NG14NN15HD16NG17NN18NN
Right: 01HD02NI03NN04HD05HD06NG07NG08NG09NG10NN11HD12NG13NN14NG15NG16NG17NI18NG

Up to 6 mismatches were allowed in each nuclease half-site.

Allowed spacing distances were: 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30

Homodimers and heterodimers of the nucleases were included in the output

2331 total sites were located
Mismatch Types
Genomic Regions
  0-0: 1
  0-1: 1
  3-5: 4
  4-4: 1
  3-6: 13
  4-5: 11
  4-6: 68
  5-5: 45
  5-6: 565
  6-6: 1622
  Exons: 51
  Promoters: 10
  Introns: 908
  Intergenic: 1362


The top 48 sites are ranked below according to a weighting of the RVD base preferences. All sites listed contain flanking 5' thymidines.
For the full output, click here.

This output will be deleted from the server one week after it is generated.  Please save your results.

Primer pairs were designed using the 422 bp of sequence on either side of the cleavage site to melt close to 60 °C

For easy visualization of cleavage on gels:
The smallest fragment will be longer than 125 bp, which is also the minimum separation distance between uncleaved and cleaved products.
The two cleavage products will be separated by a minimum size of 75 bp
Rank: 1	Homology Score: 98	RVD Match Score: 2	Left Half-Site RVD Score: 4.024	Right Half-Site RVD Score: 3.881
	Genomic Region: Exon	Closest Gene: CCR5
Match Type: L14R
	           Left Nuclease
	         TTTGTGGGCAACATGCTGG
	5'-TTGGT TTTGTGGGCAACATGCTGG         TCATCCTCATCCTG         ATAAACTGCAAAAGGCTGA AGAGC-3'
	3'-AACCA AAACACCCGTTGTACGACC         AGTAGGAGTAGGAC         TATTTGACGTTTTCCGACT TCTCG-5'
	                                                            TATTTGACGTTTTCCGACT
	                                                                  Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 003-046414377
Forward Primer: 5'-GCACAGGGTGGAACAAGATGG-3' Tm: 59.378
Reverse Primer: 5'-GTGACCGTCCTGGCTTTTAAAGCA-3' Tm: 59.038
Expected PCR Product Length: 446
Expected CelI Cleavage Product Sizes: 177, 269
In Silico PCR
Genome Browser

Rank: 2	Homology Score: 85	RVD Match Score: 2.14	Left Half-Site RVD Score: 4.024	Right Half-Site RVD Score: 5.045
	Genomic Region: Exon	Closest Gene: CCR2
Match Type: L14R
	           Left Nuclease
	         TTTGTGGGCAACATGCTGG
	5'-TTGGT TTTGTGGGCAACATGCTGG         TCGTCCTCATCTTA         ATAAACTGCAAAAAGCTGA AGTGC-3'
	3'-AACCA AAACACCCGTTGTACGACC         AGCAGGAGTAGAAT         TATTTGACGTTTTTCGACT TCACG-5'
	                                                            TATTTGACGTTTTCCGACT
	                                                                  Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 003-046399009
Forward Primer: 5'-CATCCACAACATGCTGTCCACATC-3' Tm: 59.038
Reverse Primer: 5'-CAAAGACCCACTCATTTGCAGCAG-3' Tm: 59.038
Expected PCR Product Length: 336
Expected CelI Cleavage Product Sizes: 206, 130
In Silico PCR
Genome Browser

Rank: 3	Homology Score: 17	RVD Match Score: 2.477	Left Half-Site RVD Score: 6.106	Right Half-Site RVD Score: 6.023
	Genomic Region: Intron	Closest Gene: VEPH1
Match Type: R29L
	          Right Nuclease
	         TCAGCCTTTTGCAGTTTAT
	5'-TCCAG TCAAATTTTAGCTATTTAT CCATTGCTGTGGTCCTCTGACCCCTTGGG  CCAGCAGGTTGGCCACATA GGCAC-3'
	3'-AGGTC AGTTTAAAATCGATAAATA GGTAACGACACCAGGAGACTGGGGAACCC  GGTCGTCCAACCGGTGTAT CCGTG-5'
	                                                            GGTCGTACAACGGGTGTTT
	                                                                   Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 003-157066450
Forward Primer: 5'-AAGGAAGAGGGGGCTGCAGA-3' Tm: 59.508
Reverse Primer: 5'-GCTGAGTCCTTGTCTGGTTTTGG-3' Tm: 59.148
Expected PCR Product Length: 388
Expected CelI Cleavage Product Sizes: 153, 235
In Silico PCR
Genome Browser

Rank: 4	Homology Score: 2	RVD Match Score: 2.536	Left Half-Site RVD Score: 6.343	Right Half-Site RVD Score: 6.368
	Genomic Region: Intron	Closest Gene: CNST
Match Type: R30L
	          Right Nuclease
	         TCAGCCTTTTGCAGTTTAT
	5'-TGTTT TCCATCTTTTGCTCTTTAC AAACTGGTTAAGACTGTTTCCCTCTCAGTG TCAGCAAGGTGCTTATAAA ACCTG-3'
	3'-ACAAA AGGTAGAAAACGAGAAATG TTTGACCAATTCTGACAAAGGGAGAGTCAC AGTCGTTCCACGAATATTT TGGAC-5'
	                                                            GGTCGTACAACGGGTGTTT
	                                                                   Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 001-246821429
Forward Primer: 5'-CAGCCAGATTCAAGATCCTCTCC-3' Tm: 59.148
Reverse Primer: 5'-GCTTCCCTGAATGTGGGTCTATAG-3' Tm: 59.038
Expected PCR Product Length: 394
Expected CelI Cleavage Product Sizes: 143, 251
In Silico PCR
Genome Browser

Rank: 5	Homology Score: 5	RVD Match Score: 2.554	Left Half-Site RVD Score: 5.981	Right Half-Site RVD Score: 6.69
	Genomic Region: Intergenic	Closest Gene: LINC00184
Match Type: R17R
	          Right Nuclease
	         TCAGCCTTTTGCAGTTTAT
	5'-CTGGG TCAGTCTTTTAATTTTTAT       GAGTTAGTATGCCTCTT        GTAGACAGCATAAAGTTGA ATTTT-3'
	3'-GACCC AGTCAGAAAATTAAAAATA       CTCAATCATACGGAGAA        CATCTGTCGTATTTCAACT TAAAA-5'
	                                                            TATTTGACGTTTTCCGACT
	                                                                  Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 001-234812284
Forward Primer: 5'-GCCTGCCTGCTGAAGACATAAG-3' Tm: 59.258
Reverse Primer: 5'-CGCCTGACTTTATTGAATGCAGCC-3' Tm: 59.038
Expected PCR Product Length: 486
Expected CelI Cleavage Product Sizes: 350, 136
In Silico PCR
Genome Browser

Rank: 6	Homology Score: 2	RVD Match Score: 2.564	Left Half-Site RVD Score: 6.941	Right Half-Site RVD Score: 5.842
	Genomic Region: Intergenic	Closest Gene: LARGE
Match Type: R30R
	          Right Nuclease
	         TCAGCCTTTTGCAGTTTAT
	5'-ATTTC TCATCCCTTTATTGTGTAT ATTTGAGGTTTACAACATGATGTTATAAGT ATAGATAGTAAAATGCTTA CTACA-3'
	3'-TAAAG AGTAGGGAAATAACACATA TAAACTCCAAATGTTGTACTACAATATTCA TATCTATCATTTTACGAAT GATGT-5'
	                                                            TATTTGACGTTTTCCGACT
	                                                                  Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 022-034808553
Forward Primer: 5'-CATGAGGAAGTGTCAGACAAACCC-3' Tm: 59.038
Reverse Primer: 5'-GCGGCAGAGGATCAGGAAAG-3' Tm: 59.508
Expected PCR Product Length: 669
Expected CelI Cleavage Product Sizes: 408, 261
In Silico PCR
Genome Browser

Rank: 7	Homology Score: 5	RVD Match Score: 2.569	Left Half-Site RVD Score: 8.063	Right Half-Site RVD Score: 4.943
	Genomic Region: Intergenic	Closest Gene: PVRL3-AS1
Match Type: R25R
	          Right Nuclease
	         TCAGCCTTTTGCAGTTTAT
	5'-GGTTG TAAGACTTCTACATTTTAC   TTTAAATGATAAAATATTAACTCAA    ATAAACAGTGAAATGTTGA GTAGA-3'
	3'-CCAAC ATTCTGAAGATGTAAAATG   AAATTTACTATTTTATAATTGAGTT    TATTTGTCACTTTACAACT CATCT-5'
	                                                            TATTTGACGTTTTCCGACT
	                                                                  Right Nuclease
Warning! Primer pair was generated using suboptimal parameters. 
Primer Index: 003-110537278
Forward Primer: 5'-GTGGGTCTCTCTTCACTTTATTGATTGTTTCC-3' Tm: 59.848
Reverse Primer: 5'-GGTAAGAAGAGCGAAACTCCGTC-3' Tm: 59.148
Expected PCR Product Length: 598
Expected CelI Cleavage Product Sizes: 237, 361
In Silico PCR
Genome Browser

Rank: 8	Homology Score: 10	RVD Match Score: 2.573	Left Half-Site RVD Score: 7.191	Right Half-Site RVD Score: 6.156
	Genomic Region: Intron	Closest Gene: RIMS2
Match Type: L24L
	           Left Nuclease
	         TTTGTGGGCAACATGCTGG
	5'-ATGGA TATCTCTTCAACATACTGG    AGTCTTTAGGGTTTACTGTAGATA    AGATCATGTTGTCCACAAA CAGAG-3'
	3'-TACCT ATAGAGAAGTTGTATGACC    TCAGAAATCCCAAATGACATCTAT    TCTAGTACAACAGGTGTTT GTCTC-5'
	                                                            GGTCGTACAACGGGTGTTT
	                                                                   Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 008-104699899
Forward Primer: 5'-CATACCCCAGTCTCTGGTGATC-3' Tm: 59.258
Reverse Primer: 5'-CTCAACGGTGAAAAGTTGGAAACTTTCCTC-3' Tm: 60.058
Expected PCR Product Length: 542
Expected CelI Cleavage Product Sizes: 345, 197
In Silico PCR
Genome Browser

Rank: 9	Homology Score: 5	RVD Match Score: 2.574	Left Half-Site RVD Score: 7.638	Right Half-Site RVD Score: 5.564
	Genomic Region: Intergenic	Closest Gene: PXDNL
Match Type: L20R
	           Left Nuclease
	         TTTGTGGGCAACATGCTGG
	5'-GGCTA TGTGTAGGAAACATACTTT      AGATTTAAAGAAACAAATAG      ATTAATGGTAAAAGGATGA AGAAA-3'
	3'-CCGAT ACACATCCTTTGTATGAAA      TCTAAATTTCTTTGTTTATC      TAATTACCATTTTCCTACT TCTTT-5'
	                                                            TATTTGACGTTTTCCGACT
	                                                                  Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 008-051977566
Forward Primer: 5'-CAGAGATCGCACCATTGCATTCC-3' Tm: 59.148
Reverse Primer: 5'-GTCTAAGAATCTGTGGGCAAATGTATGGTC-3' Tm: 60.058
Expected PCR Product Length: 341
Expected CelI Cleavage Product Sizes: 126, 215
In Silico PCR
Genome Browser

Rank: 10	Homology Score: 8	RVD Match Score: 2.578	Left Half-Site RVD Score: 6.363	Right Half-Site RVD Score: 6.777
	Genomic Region: Exon	Closest Gene: PPM1E
Match Type: R18L
	          Right Nuclease
	         TCAGCCTTTTGCAGTTTAT
	5'-AACTG TCTGCTTTTTGCTATTTCT       GCAGTTTCAAGTTAGTTA       GAAGCATGTTGTCAACTAA AGACA-3'
	3'-TTGAC AGACGAAAAACGATAAAGA       CGTCAAAGTTCAATCAAT       CTTCGTACAACAGTTGATT TCTGT-5'
	                                                            GGTCGTACAACGGGTGTTT
	                                                                   Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 017-057059452
Forward Primer: 5'-GTCTAAAGTGAGCCCAGAGGCC-3' Tm: 61.288
Reverse Primer: 5'-CTGCTGTTGACTAGGGGAGG-3' Tm: 59.508
Expected PCR Product Length: 325
Expected CelI Cleavage Product Sizes: 200, 125
In Silico PCR
Genome Browser

Rank: 11	Homology Score: 5	RVD Match Score: 2.583	Left Half-Site RVD Score: 6.481	Right Half-Site RVD Score: 6.952
	Genomic Region: Intergenic	Closest Gene: GRIN2A
Match Type: L18L
	           Left Nuclease
	         TTTGTGGGCAACATGCTGG
	5'-ATCCT TTTTTGTACAACATTCTAA       AAATCCTCATAGCCACTG       CCAGGAGGCTGCTCAGAAA GCTTC-3'
	3'-TAGGA AAAAACATGTTGTAAGATT       TTTAGGAGTATCGGTGAC       GGTCCTCCGACGAGTCTTT CGAAG-5'
	                                                            GGTCGTACAACGGGTGTTT
	                                                                   Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 016-009691841
Forward Primer: 5'-GATTGGAAGTCCCAGCACATGACA-3' Tm: 59.038
Reverse Primer: 5'-CCTTTGTTGCATAAGCCGTCACTG-3' Tm: 59.038
Expected PCR Product Length: 331
Expected CelI Cleavage Product Sizes: 204, 127
In Silico PCR
Genome Browser

Rank: 12	Homology Score: 17	RVD Match Score: 2.584	Left Half-Site RVD Score: 6.068	Right Half-Site RVD Score: 7.142
	Genomic Region: Intergenic	Closest Gene: FAM75C2
Match Type: L20R
	           Left Nuclease
	         TTTGTGGGCAACATGCTGG
	5'-TATGA TTTTTGGGCAACATAGTGG      GACTCTGTCTACAAAACTAA      ATAAATAGAAAGAGGCCCA GTGCG-3'
	3'-ATACT AAAAACCCGTTGTATCACC      CTGAGACAGATGTTTTGATT      TATTTATCTTTCTCCGGGT CACGC-5'
	                                                            TATTTGACGTTTTCCGACT
	                                                                  Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 009-090818650
Forward Primer: 5'-CTGCAAGGACTCAAAAAATTTGACCCCTAC-3' Tm: 60.058
Reverse Primer: 5'-TCACTATATGGGCCAGGCTGATC-3' Tm: 59.148
Expected PCR Product Length: 350
Expected CelI Cleavage Product Sizes: 225, 125
In Silico PCR
Genome Browser

Rank: 13	Homology Score: 10	RVD Match Score: 2.586	Left Half-Site RVD Score: 7.497	Right Half-Site RVD Score: 5.564
	Genomic Region: Intergenic	Closest Gene: SFT2D2
Match Type: R10R
	          Right Nuclease
	         TCAGCCTTTTGCAGTTTAT
	5'-CAAAT TAAGCCTTTTCCCTTACAT           GCATTGTTTC           ATAAAATGTAAATGGTTGA AGGGT-3'
	3'-GTTTA ATTCGGAAAAGGGAATGTA           CGTAACAAAG           TATTTTACATTTACCAACT TCCCA-5'
	                                                            TATTTGACGTTTTCCGACT
	                                                                  Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 001-168187120
Forward Primer: 5'-CCTACAGGTGTCCTCTTGTGG-3' Tm: 59.378
Reverse Primer: 5'-GCGCCCCTTCAGAGAAATGAG-3' Tm: 59.378
Expected PCR Product Length: 546
Expected CelI Cleavage Product Sizes: 186, 360
In Silico PCR
Genome Browser

Rank: 14	Homology Score: 5	RVD Match Score: 2.588	Left Half-Site RVD Score: 7.229	Right Half-Site RVD Score: 6.029
	Genomic Region: Intron	Closest Gene: LINC00536
Match Type: R14L
	          Right Nuclease
	         TCAGCCTTTTGCAGTTTAT
	5'-CAAAA TCAACCTTCTGAAGTTCCT         GTGCCTTTTAAAAA         TTAGTGTGTTTCTCACAAA ACTGA-3'
	3'-GTTTT AGTTGGAAGACTTCAAGGA         CACGGAAAATTTTT         AATCACACAAAGAGTGTTT TGACT-5'
	                                                            GGTCGTACAACGGGTGTTT
	                                                                   Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 008-117246045
Forward Primer: 5'-TTCTCTGCCACGAGTGTCTTGC-3' Tm: 59.258
Reverse Primer: 5'-TTGTCTCTGTCTTTGCCCTGCC-3' Tm: 59.258
Expected PCR Product Length: 327
Expected CelI Cleavage Product Sizes: 126, 201
In Silico PCR
Genome Browser

Rank: 15	Homology Score: 5	RVD Match Score: 2.588	Left Half-Site RVD Score: 5.989	Right Half-Site RVD Score: 7.299
	Genomic Region: Intergenic	Closest Gene: EPHA7
Match Type: R11L
	          Right Nuclease
	         TCAGCCTTTTGCAGTTTAT
	5'-AAATT TCAGCTCTTTACCATTTAT          TACTTAGTTTA           CAAGAATGTTTCATATAAA TAGTA-3'
	3'-TTTAA AGTCGAGAAATGGTAAATA          ATGAATCAAAT           GTTCTTACAAAGTATATTT ATCAT-5'
	                                                            GGTCGTACAACGGGTGTTT
	                                                                   Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 006-093167839
Forward Primer: 5'-CTCACCTCAGATTCTCTTGGGAGT-3' Tm: 59.038
Reverse Primer: 5'-CACTAAGGAATTTCCCCTCTATCAAAGGC-3' Tm: 60.178
Expected PCR Product Length: 511
Expected CelI Cleavage Product Sizes: 159, 352
In Silico PCR
Genome Browser

Rank: 16	Homology Score: 2	RVD Match Score: 2.595	Left Half-Site RVD Score: 7.021	Right Half-Site RVD Score: 6.54
	Genomic Region: Intergenic	Closest Gene: RAB11FIP2
Match Type: L24L
	           Left Nuclease
	         TTTGTGGGCAACATGCTGG
	5'-AACTC TTTGCCGGCAAGATCCTAA    AAAAGGAATTGTCCTATTCAGAAC    TCAGGGTGTTGTTCCCAAA TTCCT-3'
	3'-TTGAG AAACGGCCGTTCTAGGATT    TTTTCCTTAACAGGATAAGTCTTG    AGTCCCACAACAAGGGTTT AAGGA-5'
	                                                            GGTCGTACAACGGGTGTTT
	                                                                   Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 010-119687443
Forward Primer: 5'-ATGGGCTGTACACACCCCTG-3' Tm: 59.508
Reverse Primer: 5'-GTTGACAAAAGGAGTGGCGTATGC-3' Tm: 59.038
Expected PCR Product Length: 388
Expected CelI Cleavage Product Sizes: 234, 154
In Silico PCR
Genome Browser

Rank: 17	Homology Score: 2	RVD Match Score: 2.606	Left Half-Site RVD Score: 6.014	Right Half-Site RVD Score: 7.462
	Genomic Region: Intron	Closest Gene: SEMA3C
Match Type: R10L
	          Right Nuclease
	         TCAGCCTTTTGCAGTTTAT
	5'-CTCAC TCACCACTTTTCTATTTAT           TTTGTCCCAG           ACACCATTTTGTTCAGAAA ACAAA-3'
	3'-GAGTG AGTGGTGAAAAGATAAATA           AAACAGGGTC           TGTGGTAAAACAAGTCTTT TGTTT-5'
	                                                            GGTCGTACAACGGGTGTTT
	                                                                   Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 007-080418831
Forward Primer: 5'-GCTCCTCCTGGACACTAGAAAG-3' Tm: 59.258
Reverse Primer: 5'-CTTTTGGGAGGGCAGATCAGATG-3' Tm: 59.148
Expected PCR Product Length: 469
Expected CelI Cleavage Product Sizes: 323, 146
In Silico PCR
Genome Browser

Rank: 18	Homology Score: 5	RVD Match Score: 2.606	Left Half-Site RVD Score: 5.544	Right Half-Site RVD Score: 7.97
	Genomic Region: Intergenic	Closest Gene: ABCD2
Match Type: L12R
	           Left Nuclease
	         TTTGTGGGCAACATGCTGG
	5'-TTAGG TTTCAAGACAACATGCTAA          ATACATAGGACC          ATCTACTGGCAAAGGATGA TATAG-3'
	3'-AATCC AAAGTTCTGTTGTACGATT          TATGTATCCTGG          TAGATGACCGTTTCCTACT ATATC-5'
	                                                            TATTTGACGTTTTCCGACT
	                                                                  Right Nuclease
Warning! Primer pair was generated using suboptimal parameters. 
Primer Index: 012-039942796
Forward Primer: 5'-GAACTTTCCTGCTATGTTTATCTGAGTTTTGTGG-3' Tm: 59.668
Reverse Primer: 5'-GTCCTTGTCAGAGCCCTCATG-3' Tm: 59.378
Expected PCR Product Length: 438
Expected CelI Cleavage Product Sizes: 139, 299
In Silico PCR
Genome Browser

Rank: 19	Homology Score: 5	RVD Match Score: 2.61	Left Half-Site RVD Score: 7.605	Right Half-Site RVD Score: 5.904
	Genomic Region: Intergenic	Closest Gene: SF3B1
Match Type: R20L
	          Right Nuclease
	         TCAGCCTTTTGCAGTTTAT
	5'-AAAGT TCCGCATTTTACAAGTCAT      GTTCAAAAAACACACACAAA      TTAGCATTTTGTACACAAA TGTTT-3'
	3'-TTTCA AGGCGTAAAATGTTCAGTA      CAAGTTTTTTGTGTGTGTTT      AATCGTAAAACATGTGTTT ACAAA-5'
	                                                            GGTCGTACAACGGGTGTTT
	                                                                   Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 002-198256316
Forward Primer: 5'-CAGACCTGTCAAGTGTTGGACAAG-3' Tm: 59.038
Reverse Primer: 5'-GCCAGTAGTGACCAAGAACACAG-3' Tm: 59.148
Expected PCR Product Length: 531
Expected CelI Cleavage Product Sizes: 374, 157
In Silico PCR
Genome Browser

Rank: 20	Homology Score: 2	RVD Match Score: 2.622	Left Half-Site RVD Score: 6.951	Right Half-Site RVD Score: 6.642
	Genomic Region: Intron	Closest Gene: ZNF326
Match Type: L18R
	           Left Nuclease
	         TTTGTGGGCAACATGCTGG
	5'-ATTTT TTCTTGGACAATATGCTAA       TATTTATTGATTTCACAA       ATTTACAGCATATGGGTGA TTTTG-3'
	3'-TAAAA AAGAACCTGTTATACGATT       ATAAATAACTAAAGTGTT       TAAATGTCGTATACCCACT AAAAC-5'
	                                                            TATTTGACGTTTTCCGACT
	                                                                  Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 001-090475333
Forward Primer: 5'-GTGGTAACATTTGGACAGGCACATATGTTG-3' Tm: 60.058
Reverse Primer: 5'-CCACTGGGCTTATTAAATGGCTGC-3' Tm: 59.038
Expected PCR Product Length: 449
Expected CelI Cleavage Product Sizes: 299, 150
In Silico PCR
Genome Browser

Rank: 21	Homology Score: 13	RVD Match Score: 2.624	Left Half-Site RVD Score: 6.843	Right Half-Site RVD Score: 6.767
	Genomic Region: Intergenic	Closest Gene: KDM5D
Match Type: R25L
	          Right Nuclease
	         TCAGCCTTTTGCAGTTTAT
	5'-TATGG TAAGCACTTTGTTGTTTAT   GGGCTTCTTTTCCACCTATCAATTC    CCAGTTGGTTGCCCCCAAA ATGAA-3'
	3'-ATACC ATTCGTGAAACAACAAATA   CCCGAAGAAAAGGTGGATAGTTAAG    GGTCAACCAACGGGGGTTT TACTT-5'
	                                                            GGTCGTACAACGGGTGTTT
	                                                                   Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 00Y-022149362
Forward Primer: 5'-CATGACTGACACCCTCCACG-3' Tm: 59.508
Reverse Primer: 5'-AGCAAAGCAGGTGGCACAGC-3' Tm: 59.508
Expected PCR Product Length: 330
Expected CelI Cleavage Product Sizes: 203, 127
In Silico PCR
Genome Browser

Rank: 22	Homology Score: 5	RVD Match Score: 2.629	Left Half-Site RVD Score: 7.053	Right Half-Site RVD Score: 6.373
	Genomic Region: Intron	Closest Gene: PDE1C
Match Type: R13R
	          Right Nuclease
	         TCAGCCTTTTGCAGTTTAT
	5'-CTCTC TCTGCCTTTTTCTCTCTCT         TTCCCCCCACAAC          ACACACATCAAAAGGTTGA GCTTG-3'
	3'-GAGAG AGACGGAAAAAGAGAGAGA         AAGGGGGGTGTTG          TGTGTGTAGTTTTCCAACT CGAAC-5'
	                                                            TATTTGACGTTTTCCGACT
	                                                                  Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 007-032193295
Forward Primer: 5'-GATAGAGCCCACGTAGTGTAGTC-3' Tm: 59.148
Reverse Primer: 5'-CTGCACTGGACTCCCAAAATACTG-3' Tm: 59.038
Expected PCR Product Length: 345
Expected CelI Cleavage Product Sizes: 210, 135
In Silico PCR
Genome Browser

Rank: 23	Homology Score: 5	RVD Match Score: 2.631	Left Half-Site RVD Score: 5.977	Right Half-Site RVD Score: 7.503
	Genomic Region: Intergenic	Closest Gene: GUCY1A2
Match Type: R20R
	          Right Nuclease
	         TCAGCCTTTTGCAGTTTAT
	5'-CTTCC TCACCATCATGCTGTTTAT      ATTAGGCTTTAAAAATATTA      CTTAATGGCAATATGCTGA AACCT-3'
	3'-GAAGG AGTGGTAGTACGACAAATA      TAATCCGAAATTTTTATAAT      GAATTACCGTTATACGACT TTGGA-5'
	                                                            TATTTGACGTTTTCCGACT
	                                                                  Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 011-106518241
Forward Primer: 5'-CCAGTTTGACCCATACTACTGAGTCTCT-3' Tm: 60.298
Reverse Primer: 5'-GGGATGACACATAGTGCCATCTG-3' Tm: 59.148
Expected PCR Product Length: 334
Expected CelI Cleavage Product Sizes: 126, 208
In Silico PCR
Genome Browser

Rank: 24	Homology Score: 5	RVD Match Score: 2.635	Left Half-Site RVD Score: 8.3	Right Half-Site RVD Score: 5.787
	Genomic Region: Intergenic	Closest Gene: EXOC6B
Match Type: L10L
	           Left Nuclease
	         TTTGTGGGCAACATGCTGG
	5'-AGATC TTTATGGGCTTCATAAAGG           ATGAAGAAAT           TCTGTATGTTGCCAATAAA ATATA-3'
	3'-TCTAG AAATACCCGAAGTATTTCC           TACTTCTTTA           AGACATACAACGGTTATTT TATAT-5'
	                                                            GGTCGTACAACGGGTGTTT
	                                                                   Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 002-072399256
Forward Primer: 5'-CTCCCAAAGTGCTGGGATTACAGG-3' Tm: 60.908
Reverse Primer: 5'-CCCTAATCCCATTTCCCACATCTG-3' Tm: 59.038
Expected PCR Product Length: 457
Expected CelI Cleavage Product Sizes: 266, 191
In Silico PCR
Genome Browser

Rank: 25	Homology Score: 13	RVD Match Score: 2.637	Left Half-Site RVD Score: 8.507	Right Half-Site RVD Score: 5.194
	Genomic Region: Intron	Closest Gene: MAP3K5
Match Type: R27R
	          Right Nuclease
	         TCAGCCTTTTGCAGTTTAT
	5'-ATACC TCCTCCCTTTGGAGTTTAG  GCACAACTGACCAGCATTACCATTAAA   ATAAAGATCATAAGGCTGA CAAAA-3'
	3'-TATGG AGGAGGGAAACCTCAAATC  CGTGTTGACTGGTCGTAATGGTAATTT   TATTTCTAGTATTCCGACT GTTTT-5'
	                                                            TATTTGACGTTTTCCGACT
	                                                                  Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 006-136890071
Forward Primer: 5'-GAAGAACCACCTACCCCCAG-3' Tm: 59.508
Reverse Primer: 5'-CTACAGAATGCAGATTTCCCCCAC-3' Tm: 59.038
Expected PCR Product Length: 348
Expected CelI Cleavage Product Sizes: 135, 213
In Silico PCR
Genome Browser

Rank: 26	Homology Score: 2	RVD Match Score: 2.637	Left Half-Site RVD Score: 6.426	Right Half-Site RVD Score: 7.343
	Genomic Region: Intron	Closest Gene: FAM160A1
Match Type: R30L
	          Right Nuclease
	         TCAGCCTTTTGCAGTTTAT
	5'-TGTAC TCAGCATAATACTGATTAT ATTATATAGTTTCAAGTTTTCTTTTATTTT AAAGCATGTTTTCTTCAAA TGAAG-3'
	3'-ACATG AGTCGTATTATGACTAATA TAATATATCAAAGTTCAAAAGAAAATAAAA TTTCGTACAAAAGAAGTTT ACTTC-5'
	                                                            GGTCGTACAACGGGTGTTT
	                                                                   Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 004-152360042
Forward Primer: 5'-CACCTCAGCCTCCGGAATTTTG-3' Tm: 59.258
Reverse Primer: 5'-ACTCCTTTAGTCCCCACAACAACC-3' Tm: 59.038
Expected PCR Product Length: 411
Expected CelI Cleavage Product Sizes: 168, 243
In Silico PCR
Genome Browser

Rank: 27	Homology Score: 2	RVD Match Score: 2.638	Left Half-Site RVD Score: 6.987	Right Half-Site RVD Score: 6.528
	Genomic Region: Intron	Closest Gene: PCDH11Y
Match Type: R20R
	          Right Nuclease
	         TCAGCCTTTTGCAGTTTAT
	5'-TGTTC TCAGCCTTTCTCTGTCACT      AGGCTCAGTAGGAAGGAAGG      ATAAATGGCAAATGTTGGA TGGGA-3'
	3'-ACAAG AGTCGGAAAGAGACAGTGA      TCCGAGTCATCCTTCCTTCC      TATTTACCGTTTACAACCT ACCCT-5'
	                                                            TATTTGACGTTTTCCGACT
	                                                                  Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 00Y-005558821
Forward Primer: 5'-GCAGGCTTACTTGAAGTCCCAG-3' Tm: 59.258
Reverse Primer: 5'-CGTGCCTACTAAGAACTGCTCTC-3' Tm: 59.148
Expected PCR Product Length: 683
Expected CelI Cleavage Product Sizes: 253, 430
In Silico PCR
Genome Browser

Rank: 28	Homology Score: 10	RVD Match Score: 2.641	Left Half-Site RVD Score: 6.159	Right Half-Site RVD Score: 7.684
	Genomic Region: Intron	Closest Gene: KLHDC1
Match Type: R16L
	          Right Nuclease
	         TCAGCCTTTTGCAGTTTAT
	5'-AGCGC TCAGCCTTTTAAAATTTTT        TGTAGGGATGGGGATC        TCACTATGTTGCCCAGGCA GACCT-3'
	3'-TCGCG AGTCGGAAAATTTTAAAAA        ACATCCCTACCCCTAG        AGTGATACAACGGGTCCGT CTGGA-5'
	                                                            GGTCGTACAACGGGTGTTT
	                                                                   Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 014-050186827
Forward Primer: 5'-CCTGTGTAGCTGGGATTACAGGC-3' Tm: 61.088
Reverse Primer: 5'-AAGATGGAGGCCAGACTGGG-3' Tm: 59.508
Expected PCR Product Length: 325
Expected CelI Cleavage Product Sizes: 200, 125
In Silico PCR
Genome Browser

Rank: 29	Homology Score: 5	RVD Match Score: 2.642	Left Half-Site RVD Score: 6.222	Right Half-Site RVD Score: 7.875
	Genomic Region: Intergenic	Closest Gene: MBIP
Match Type: L28L
	           Left Nuclease
	         TTTGTGGGCAACATGCTGG
	5'-TGAGG TTTATAGTCACCATCCTAG  ACCATGTCGTGGGATTTTCCCTTGAATC  CCAGCATGTAGTACAAACA TGTGG-3'
	3'-ACTCC AAATATCAGTGGTAGGATC  TGGTACAGCACCCTAAAAGGGAACTTAG  GGTCGTACATCATGTTTGT ACACC-5'
	                                                            GGTCGTACAACGGGTGTTT
	                                                                   Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 014-036739807
Forward Primer: 5'-GAGGGATCCAGGATGACAAAGG-3' Tm: 59.258
Reverse Primer: 5'-CTGTCTATTCCATCTCAGGGGTTC-3' Tm: 59.038
Expected PCR Product Length: 335
Expected CelI Cleavage Product Sizes: 129, 206
In Silico PCR
Genome Browser

Rank: 30	Homology Score: 13	RVD Match Score: 2.643	Left Half-Site RVD Score: 6.497	Right Half-Site RVD Score: 7.318
	Genomic Region: Intergenic	Closest Gene: LOC728084
Match Type: L17R
	           Left Nuclease
	         TTTGTGGGCAACATGCTGG
	5'-CTTTA TCTGAGGGCAACATCCTAC       CACAATTCCCATAATAA        ATAGACTGCACAAGAGTGA GAAGC-3'
	3'-GAAAT AGACTCCCGTTGTAGGATG       GTGTTAAGGGTATTATT        TATCTGACGTGTTCTCACT CTTCG-5'
	                                                            TATTTGACGTTTTCCGACT
	                                                                  Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 012-089421427
Forward Primer: 5'-GCATTCACTCGCCTGTTCCTC-3' Tm: 59.378
Reverse Primer: 5'-GCTGACTTAAGAGGGCTCTTTGC-3' Tm: 59.148
Expected PCR Product Length: 332
Expected CelI Cleavage Product Sizes: 125, 207
In Silico PCR
Genome Browser

Rank: 31	Homology Score: 10	RVD Match Score: 2.647	Left Half-Site RVD Score: 6.039	Right Half-Site RVD Score: 7.854
	Genomic Region: Intergenic	Closest Gene: POM121L12
Match Type: L26R
	           Left Nuclease
	         TTTGTGGGCAACATGCTGG
	5'-AAAAA TTTGTGGGCAACATTCACA   AAAGAATGGATTCCATGAGAACAGAA   AAAAAAAGAAAAAGTCTAA GACAT-3'
	3'-TTTTT AAACACCCGTTGTAAGTGT   TTTCTTACCTAAGGTACTCTTGTCTT   TTTTTTTCTTTTTCAGATT CTGTA-5'
	                                                            TATTTGACGTTTTCCGACT
	                                                                  Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 007-052877054
Forward Primer: 5'-GCAACTTAGGGCACGTGACC-3' Tm: 59.508
Reverse Primer: 5'-TCCCCTTGCAACAGCACATGTTAG-3' Tm: 59.038
Expected PCR Product Length: 325
Expected CelI Cleavage Product Sizes: 200, 125
In Silico PCR
Genome Browser

Rank: 32	Homology Score: 10	RVD Match Score: 2.65	Left Half-Site RVD Score: 5.159	Right Half-Site RVD Score: 8.709
	Genomic Region: Intergenic	Closest Gene: MIR4422
Match Type: R21R
	          Right Nuclease
	         TCAGCCTTTTGCAGTTTAT
	5'-GGGAG TCAGCCTTTTCCCATATAT     AAAACAGGGTTACAATCCTGT      ATAAACTTTTAAAAGATAA TAGTT-3'
	3'-CCCTC AGTCGGAAAAGGGTATATA     TTTTGTCCCAATGTTAGGACA      TATTTGAAAATTTTCTATT ATCAA-5'
	                                                            TATTTGACGTTTTCCGACT
	                                                                  Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 001-055972723
Forward Primer: 5'-ACCCCCAAATACCCTTTTCCCC-3' Tm: 59.258
Reverse Primer: 5'-GGGTGTCGGCCTGAATTACATTG-3' Tm: 59.148
Expected PCR Product Length: 328
Expected CelI Cleavage Product Sizes: 201, 127
In Silico PCR
Genome Browser

Rank: 33	Homology Score: 10	RVD Match Score: 2.651	Left Half-Site RVD Score: 6.132	Right Half-Site RVD Score: 7.79
	Genomic Region: Intergenic	Closest Gene: OR7E156P
Match Type: L28R
	           Left Nuclease
	         TTTGTGGGCAACATGCTGG
	5'-TTTTT TTAATGGGCAACATATTGG  AAGACACTTTGCAAAGGCATACGTACCA  ATAATCAACAAATGCATGA AAAGT-3'
	3'-AAAAA AATTACCCGTTGTATAACC  TTCTGTGAAACGTTTCCGTATGCATGGT  TATTAGTTGTTTACGTACT TTTCA-5'
	                                                            TATTTGACGTTTTCCGACT
	                                                                  Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 013-064006601
Forward Primer: 5'-CAGCATCCCGAGTAACCAGG-3' Tm: 59.508
Reverse Primer: 5'-GAGTTCCAGTTACTCTTCCTCTCC-3' Tm: 59.038
Expected PCR Product Length: 398
Expected CelI Cleavage Product Sizes: 237, 161
In Silico PCR
Genome Browser

Rank: 34	Homology Score: 2	RVD Match Score: 2.651	Left Half-Site RVD Score: 6.787	Right Half-Site RVD Score: 6.86
	Genomic Region: Intergenic	Closest Gene: ARAP2
Match Type: R13R
	          Right Nuclease
	         TCAGCCTTTTGCAGTTTAT
	5'-TGTTG TATACCTTTTCCTGTGTAT         TTTCTGATCGAAA          TCAAGCATCAAAAGGTTGA GTTGC-3'
	3'-ACAAC ATATGGAAAAGGACACATA         AAAGACTAGCTTT          AGTTCGTAGTTTTCCAACT CAACG-5'
	                                                            TATTTGACGTTTTCCGACT
	                                                                  Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 004-035914090
Forward Primer: 5'-GCCAGAAGCTGCCACTATGC-3' Tm: 59.508
Reverse Primer: 5'-GCACCTACATGCAGTGCACAG-3' Tm: 59.378
Expected PCR Product Length: 330
Expected CelI Cleavage Product Sizes: 202, 128
In Silico PCR
Genome Browser

Rank: 35	Homology Score: 5	RVD Match Score: 2.652	Left Half-Site RVD Score: 6.705	Right Half-Site RVD Score: 7.203
	Genomic Region: Intron	Closest Gene: AFF3
Match Type: R27L
	          Right Nuclease
	         TCAGCCTTTTGCAGTTTAT
	5'-TGCTT TAAACCTTTAAAAATTTAT  TTTTAGGCTTAAAACCTAGGAGGAGGA   CAGACATGTTGCCTAGAAA TAAAA-3'
	3'-ACGAA ATTTGGAAATTTTTAAATA  AAAATCCGAATTTTGGATCCTCCTCCT   GTCTGTACAACGGATCTTT ATTTT-5'
	                                                            GGTCGTACAACGGGTGTTT
	                                                                   Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 002-100505280
Forward Primer: 5'-GGGATGAACAATGACGGCAACAAG-3' Tm: 59.038
Reverse Primer: 5'-CTGGACAGCACAGTGAGACC-3' Tm: 59.508
Expected PCR Product Length: 357
Expected CelI Cleavage Product Sizes: 231, 126
In Silico PCR
Genome Browser

Rank: 36	Homology Score: 5	RVD Match Score: 2.652	Left Half-Site RVD Score: 6.351	Right Half-Site RVD Score: 7.59
	Genomic Region: Intergenic	Closest Gene: METTL15
Match Type: R19L
	          Right Nuclease
	         TCAGCCTTTTGCAGTTTAT
	5'-CTCTG TCAGCTTTTTGGTATTAAT      TTTCAAACAGTTTTCTTTC       CCATCAGTTGGTCCATAAA ACTAG-3'
	3'-GAGAC AGTCGAAAAACCATAATTA      AAAGTTTGTCAAAAGAAAG       GGTAGTCAACCAGGTATTT TGATC-5'
	                                                            GGTCGTACAACGGGTGTTT
	                                                                   Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 011-029003196
Forward Primer: 5'-GCCATTGCACATACAGACAGTGAG-3' Tm: 59.038
Reverse Primer: 5'-GTAGCACACCTAAGAATCTAGCCC-3' Tm: 59.038
Expected PCR Product Length: 353
Expected CelI Cleavage Product Sizes: 130, 223
In Silico PCR
Genome Browser

Rank: 37	Homology Score: 5	RVD Match Score: 2.653	Left Half-Site RVD Score: 5.903	Right Half-Site RVD Score: 7.826
	Genomic Region: Intron	Closest Gene: ZSCAN30
Match Type: R14R
	          Right Nuclease
	         TCAGCCTTTTGCAGTTTAT
	5'-TTATG TCAGCCATCTACTAGTTAT         ATCTAGAGATTTCT         AGTAACTAGAAATGGCTGA TATAA-3'
	3'-AATAC AGTCGGTAGATGATCAATA         TAGATCTCTAAAGA         TCATTGATCTTTACCGACT ATATT-5'
	                                                            TATTTGACGTTTTCCGACT
	                                                                  Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 018-032835074
Forward Primer: 5'-GGGGATGGAGACAACTCCTTC-3' Tm: 59.378
Reverse Primer: 5'-TAGCTTCTCTGAGCCTCAGTTTCC-3' Tm: 59.038
Expected PCR Product Length: 338
Expected CelI Cleavage Product Sizes: 213, 125
In Silico PCR
Genome Browser

Rank: 38	Homology Score: 2	RVD Match Score: 2.654	Left Half-Site RVD Score: 8.219	Right Half-Site RVD Score: 5.585
	Genomic Region: Intergenic	Closest Gene: CDH10
Match Type: R24R
	          Right Nuclease
	         TCAGCCTTTTGCAGTTTAT
	5'-ATGCA TCACCTCTCTCCAGCTTAT    TACAGACATGATCAACATTTGGTC    AGGAATAGAAAAAGGTTGA TATGT-3'
	3'-TACGT AGTGGAGAGAGGTCGAATA    ATGTCTGTACTAGTTGTAAACCAG    TCCTTATCTTTTTCCAACT ATACA-5'
	                                                            TATTTGACGTTTTCCGACT
	                                                                  Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 005-024257673
Forward Primer: 5'-CACTTCAGCCTTTCAAAGTGCTGG-3' Tm: 59.038
Reverse Primer: 5'-GAGATACTGGTTCCCCCTCC-3' Tm: 59.508
Expected PCR Product Length: 333
Expected CelI Cleavage Product Sizes: 208, 125
In Silico PCR
Genome Browser

Rank: 39	Homology Score: 10	RVD Match Score: 2.654	Left Half-Site RVD Score: 5.893	Right Half-Site RVD Score: 7.854
	Genomic Region: Exon	Closest Gene: PLP1
Match Type: R14R
	          Right Nuclease
	         TCAGCCTTTTGCAGTTTAT
	5'-AGATT TCAGTCTTTTCCTGTTTTT         GTTAACTTTGGGTT         AAAAAAAAAAAAAGTCTGA TTGGT-3'
	3'-TCTAA AGTCAGAAAAGGACAAAAA         CAATTGAAACCCAA         TTTTTTTTTTTTTCAGACT AACCA-5'
	                                                            TATTTGACGTTTTCCGACT
	                                                                  Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 00X-103046628
Forward Primer: 5'-GGGTTGCATCAGAAATGTCTGGAG-3' Tm: 59.038
Reverse Primer: 5'-CCTTCTATGCAGCCTGGAAGC-3' Tm: 59.378
Expected PCR Product Length: 620
Expected CelI Cleavage Product Sizes: 416, 204
In Silico PCR
Genome Browser

Rank: 40	Homology Score: 17	RVD Match Score: 2.659	Left Half-Site RVD Score: 7.14	Right Half-Site RVD Score: 6.589
	Genomic Region: Intergenic	Closest Gene: FAM89A
Match Type: R29R
	          Right Nuclease
	         TCAGCCTTTTGCAGTTTAT
	5'-TTCTG TCAGTCTTATGATCTCTAT TTAACATTAATGCTGGTTAGTCATTGTGT  CTAAACTGCAAAAGGGAGA GGTTA-3'
	3'-AAGAC AGTCAGAATACTAGAGATA AATTGTAATTACGACCAATCAGTAACACA  GATTTGACGTTTTCCCTCT CCAAT-5'
	                                                            TATTTGACGTTTTCCGACT
	                                                                  Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 001-231235899
Forward Primer: 5'-CTTGAGGCCAGTGCTTGTTTAGG-3' Tm: 59.148
Reverse Primer: 5'-TCCCCCTCTTGGCCAAAAGC-3' Tm: 59.508
Expected PCR Product Length: 326
Expected CelI Cleavage Product Sizes: 200, 126
In Silico PCR
Genome Browser

Rank: 41	Homology Score: 17	RVD Match Score: 2.659	Left Half-Site RVD Score: 6.589	Right Half-Site RVD Score: 7.14
	Genomic Region: Intergenic	Closest Gene: GXYLT1
Match Type: R24R
	          Right Nuclease
	         TCAGCCTTTTGCAGTTTAT
	5'-TACCC TCTCCCTTTTGCAGTTTAG    ACACAATTCACCAGCATTAGTAAA    ATAGAGATCATAAGACTGA CAGAA-3'
	3'-ATGGG AGAGGGAAAACGTCAAATC    TGTGTTAAGTGGTCGTAATCATTT    TATCTCTAGTATTCTGACT GTCTT-5'
	                                                            TATTTGACGTTTTCCGACT
	                                                                  Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 012-042468830
Forward Primer: 5'-GAATGGACACCTACCTTGGCC-3' Tm: 59.378
Reverse Primer: 5'-GTCTGGCAATGGATCAGCTGCA-3' Tm: 59.258
Expected PCR Product Length: 362
Expected CelI Cleavage Product Sizes: 130, 232
In Silico PCR
Genome Browser

Rank: 42	Homology Score: 17	RVD Match Score: 2.659	Left Half-Site RVD Score: 7.14	Right Half-Site RVD Score: 6.598
	Genomic Region: Intron	Closest Gene: ACER3
Match Type: R27R
	          Right Nuclease
	         TCAGCCTTTTGCAGTTTAT
	5'-TTTTG TCAGTCTTATGATCTCTAT  TTTAACCTTAATCATGGGCAGTTGTGC   CTAAACTCCAAAAGGGTGA GGATT-3'
	3'-AAAAC AGTCAGAATACTAGAGATA  AAATTGGAATTAGTACCCGTCAACACG   GATTTGAGGTTTTCCCACT CCTAA-5'
	                                                            TATTTGACGTTTTCCGACT
	                                                                  Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 011-076680948
Forward Primer: 5'-CCTGCATATTGTGCACATGTACCC-3' Tm: 59.038
Reverse Primer: 5'-GGATGAACCCCTCTTGGCCA-3' Tm: 59.508
Expected PCR Product Length: 355
Expected CelI Cleavage Product Sizes: 224, 131
In Silico PCR
Genome Browser

Rank: 43	Homology Score: 5	RVD Match Score: 2.66	Left Half-Site RVD Score: 6.883	Right Half-Site RVD Score: 7.357
	Genomic Region: Intron	Closest Gene: PRH1-PRR4
Match Type: L27L
	           Left Nuclease
	         TTTGTGGGCAACATGCTGG
	5'-TGTCA TTTGTGGGAAAAATAATGA  CGTATGCTTATATACATATAAATATTT   CCAGGAGGATGTTCAAAAA ATTAA-3'
	3'-ACAGT AAACACCCTTTTTATTACT  GCATACGAATATATGTATATTTATAAA   GGTCCTCCTACAAGTTTTT TAATT-5'
	                                                            GGTCGTACAACGGGTGTTT
	                                                                   Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 012-010998876
Forward Primer: 5'-CCAAGAAGATCTATAGGTCTCCGTGAAATG-3' Tm: 60.058
Reverse Primer: 5'-GGTCCAGTGTCCCTGCTAAATG-3' Tm: 59.258
Expected PCR Product Length: 361
Expected CelI Cleavage Product Sizes: 133, 228
In Silico PCR
Genome Browser

Rank: 44	Homology Score: 5	RVD Match Score: 2.661	Left Half-Site RVD Score: 6.281	Right Half-Site RVD Score: 8.029
	Genomic Region: Intron	Closest Gene: HIVEP3
Match Type: L29L
	           Left Nuclease
	         TTTGTGGGCAACATGCTGG
	5'-AGGAG TTTCTGAGCAACACAATGG ACAACTTTCTCTACTGTTCCGCCGATGTT  CTGAGGTGTTGCCCACATA GACAG-3'
	3'-TCCTC AAAGACTCGTTGTGTTACC TGTTGAAAGAGATGACAAGGCGGCTACAA  GACTCCACAACGGGTGTAT CTGTC-5'
	                                                            GGTCGTACAACGGGTGTTT
	                                                                   Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 001-042172353
Forward Primer: 5'-GTAGGAGGGGACCAGGAAAC-3' Tm: 59.508
Reverse Primer: 5'-CTGGCTAAAACTCACTGGTCAGCT-3' Tm: 59.038
Expected PCR Product Length: 339
Expected CelI Cleavage Product Sizes: 125, 214
In Silico PCR
Genome Browser

Rank: 45	Homology Score: 5	RVD Match Score: 2.662	Left Half-Site RVD Score: 7.907	Right Half-Site RVD Score: 5.925
	Genomic Region: Intron	Closest Gene: CLASP2
Match Type: R26R
	          Right Nuclease
	         TCAGCCTTTTGCAGTTTAT
	5'-TCCAG TGAGACTTTTGTTTTTTAA   ACTGAAGAACCCAGCCACGTTAAGAG   ATAAACAGAAAGAGCTTGA ACCTA-3'
	3'-AGGTC ACTCTGAAAACAAAAAATT   TGACTTCTTGGGTCGGTGCAATTCTC   TATTTGTCTTTCTCGAACT TGGAT-5'
	                                                            TATTTGACGTTTTCCGACT
	                                                                  Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 003-033670533
Forward Primer: 5'-GGTGCCTCAACGATGATCAGTG-3' Tm: 59.258
Reverse Primer: 5'-GTCCTTCTTCAAGGGCCAGC-3' Tm: 59.508
Expected PCR Product Length: 345
Expected CelI Cleavage Product Sizes: 218, 127
In Silico PCR
Genome Browser

Rank: 46	Homology Score: 2	RVD Match Score: 2.663	Left Half-Site RVD Score: 7.157	Right Half-Site RVD Score: 6.617
	Genomic Region: Intergenic	Closest Gene: ITGA6
Match Type: R22R
	          Right Nuclease
	         TCAGCCTTTTGCAGTTTAT
	5'-CTGGC TCTGCCCCTTACCCTTTAT     GGGTCTCAGTTTCCTTAACTCT     ATACATAGGAGAAGACTGA ATTTT-3'
	3'-GACCG AGACGGGGAATGGGAAATA     CCCAGAGTCAAAGGAATTGAGA     TATGTATCCTCTTCTGACT TAAAA-5'
	                                                            TATTTGACGTTTTCCGACT
	                                                                  Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 002-173274458
Forward Primer: 5'-CCCTATGAATCCTAGTGTGTTGCG-3' Tm: 59.038
Reverse Primer: 5'-CAGGAGTTCAAGGTTACAGTGAGC-3' Tm: 59.038
Expected PCR Product Length: 384
Expected CelI Cleavage Product Sizes: 150, 234
In Silico PCR
Genome Browser

Rank: 47	Homology Score: 2	RVD Match Score: 2.663	Left Half-Site RVD Score: 7.615	Right Half-Site RVD Score: 6.192
	Genomic Region: Intron	Closest Gene: SCHIP1
Match Type: R25R
	          Right Nuclease
	         TCAGCCTTTTGCAGTTTAT
	5'-GCACA TCAGCCTATCAGAATTCAT   GGGAGGTTATTTCTTAAGTGAAATT    CTAAAGAGTTAAAGGATGA ATTTG-3'
	3'-CGTGT AGTCGGATAGTCTTAAGTA   CCCTCCAATAAAGAATTCACTTTAA    GATTTCTCAATTTCCTACT TAAAC-5'
	                                                            TATTTGACGTTTTCCGACT
	                                                                  Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 003-159570943
Forward Primer: 5'-AGAAAACAGCAGCTGCCCGG-3' Tm: 59.508
Reverse Primer: 5'-AAAAGAGCTCTCCCCCCTTACC-3' Tm: 59.258
Expected PCR Product Length: 326
Expected CelI Cleavage Product Sizes: 200, 126
In Silico PCR
Genome Browser

Rank: 48	Homology Score: 5	RVD Match Score: 2.664	Left Half-Site RVD Score: 6.693	Right Half-Site RVD Score: 7.085
	Genomic Region: Intergenic	Closest Gene: MIR1246
Match Type: R14R
	          Right Nuclease
	         TCAGCCTTTTGCAGTTTAT
	5'-TGCAT TCAGGCTTTTCCTTTTTGT         GGCAATCAACAGTT         ATAATCAGCAAGTGGGTCA CTGCT-3'
	3'-ACGTA AGTCCGAAAAGGAAAAACA         CCGTTAGTTGTCAA         TATTAGTCGTTCACCCAGT GACGA-5'
	                                                            TATTTGACGTTTTCCGACT
	                                                                  Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 002-177354353
Forward Primer: 5'-TGAGCAGCAGCCCCTCTACT-3' Tm: 59.508
Reverse Primer: 5'-AAGAGTGACTGCACCCAGGC-3' Tm: 59.508
Expected PCR Product Length: 333
Expected CelI Cleavage Product Sizes: 129, 204
In Silico PCR
Genome Browser

Plate format Oligo Order Form
Well
Name
Sequence
5' mods
Internal
Notes
Primer Plate #1
A1
003-046414377F
GCACAGGGTGGAACAAGATGG
12212012SXJ93rlv_QUEUE_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
A2
003-046414377R
GTGACCGTCCTGGCTTTTAAAGCA
12212012SXJ93rlv_QUEUE_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
B1
003-046399009F
CATCCACAACATGCTGTCCACATC
12212012SXJ93rlv_QUEUE_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
B2
003-046399009R
CAAAGACCCACTCATTTGCAGCAG
12212012SXJ93rlv_QUEUE_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
C1
003-157066450F
AAGGAAGAGGGGGCTGCAGA
12212012SXJ93rlv_QUEUE_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
C2
003-157066450R
GCTGAGTCCTTGTCTGGTTTTGG
12212012SXJ93rlv_QUEUE_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
D1
001-246821429F
CAGCCAGATTCAAGATCCTCTCC
12212012SXJ93rlv_QUEUE_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
D2
001-246821429R
GCTTCCCTGAATGTGGGTCTATAG
12212012SXJ93rlv_QUEUE_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
E1
001-234812284F
GCCTGCCTGCTGAAGACATAAG
12212012SXJ93rlv_QUEUE_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
E2
001-234812284R
CGCCTGACTTTATTGAATGCAGCC
12212012SXJ93rlv_QUEUE_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
F1
022-034808553F
CATGAGGAAGTGTCAGACAAACCC
12212012SXJ93rlv_QUEUE_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
F2
022-034808553R
GCGGCAGAGGATCAGGAAAG
12212012SXJ93rlv_QUEUE_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
G1
003-110537278F
GTGGGTCTCTCTTCACTTTATTGATTGTTTCC
12212012SXJ93rlv_QUEUE_Ranking-RVDs_5T	Warning! Primer pair was generated using suboptimal parameters. 
G2
003-110537278R
GGTAAGAAGAGCGAAACTCCGTC
12212012SXJ93rlv_QUEUE_Ranking-RVDs_5T	Warning! Primer pair was generated using suboptimal parameters. 
H1
008-104699899F
CATACCCCAGTCTCTGGTGATC
12212012SXJ93rlv_QUEUE_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
H2
008-104699899R
CTCAACGGTGAAAAGTTGGAAACTTTCCTC
12212012SXJ93rlv_QUEUE_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
A3
008-051977566F
CAGAGATCGCACCATTGCATTCC
12212012SXJ93rlv_QUEUE_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
A4
008-051977566R
GTCTAAGAATCTGTGGGCAAATGTATGGTC
12212012SXJ93rlv_QUEUE_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
B3
017-057059452F
GTCTAAAGTGAGCCCAGAGGCC
12212012SXJ93rlv_QUEUE_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
B4
017-057059452R
CTGCTGTTGACTAGGGGAGG
12212012SXJ93rlv_QUEUE_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
C3
016-009691841F
GATTGGAAGTCCCAGCACATGACA
12212012SXJ93rlv_QUEUE_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
C4
016-009691841R
CCTTTGTTGCATAAGCCGTCACTG
12212012SXJ93rlv_QUEUE_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
D3
009-090818650F
CTGCAAGGACTCAAAAAATTTGACCCCTAC
12212012SXJ93rlv_QUEUE_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
D4
009-090818650R
TCACTATATGGGCCAGGCTGATC
12212012SXJ93rlv_QUEUE_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
E3
001-168187120F
CCTACAGGTGTCCTCTTGTGG
12212012SXJ93rlv_QUEUE_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
E4
001-168187120R
GCGCCCCTTCAGAGAAATGAG
12212012SXJ93rlv_QUEUE_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
F3
008-117246045F
TTCTCTGCCACGAGTGTCTTGC
12212012SXJ93rlv_QUEUE_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
F4
008-117246045R
TTGTCTCTGTCTTTGCCCTGCC
12212012SXJ93rlv_QUEUE_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
G3
006-093167839F
CTCACCTCAGATTCTCTTGGGAGT
12212012SXJ93rlv_QUEUE_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
G4
006-093167839R
CACTAAGGAATTTCCCCTCTATCAAAGGC
12212012SXJ93rlv_QUEUE_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
H3
010-119687443F
ATGGGCTGTACACACCCCTG
12212012SXJ93rlv_QUEUE_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
H4
010-119687443R
GTTGACAAAAGGAGTGGCGTATGC
12212012SXJ93rlv_QUEUE_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
A5
007-080418831F
GCTCCTCCTGGACACTAGAAAG
12212012SXJ93rlv_QUEUE_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
A6
007-080418831R
CTTTTGGGAGGGCAGATCAGATG
12212012SXJ93rlv_QUEUE_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
B5
012-039942796F
GAACTTTCCTGCTATGTTTATCTGAGTTTTGTGG
12212012SXJ93rlv_QUEUE_Ranking-RVDs_5T	Warning! Primer pair was generated using suboptimal parameters. 
B6
012-039942796R
GTCCTTGTCAGAGCCCTCATG
12212012SXJ93rlv_QUEUE_Ranking-RVDs_5T	Warning! Primer pair was generated using suboptimal parameters. 
C5
002-198256316F
CAGACCTGTCAAGTGTTGGACAAG
12212012SXJ93rlv_QUEUE_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
C6
002-198256316R
GCCAGTAGTGACCAAGAACACAG
12212012SXJ93rlv_QUEUE_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
D5
001-090475333F
GTGGTAACATTTGGACAGGCACATATGTTG
12212012SXJ93rlv_QUEUE_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
D6
001-090475333R
CCACTGGGCTTATTAAATGGCTGC
12212012SXJ93rlv_QUEUE_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
E5
00Y-022149362F
CATGACTGACACCCTCCACG
12212012SXJ93rlv_QUEUE_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
E6
00Y-022149362R
AGCAAAGCAGGTGGCACAGC
12212012SXJ93rlv_QUEUE_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
F5
007-032193295F
GATAGAGCCCACGTAGTGTAGTC
12212012SXJ93rlv_QUEUE_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
F6
007-032193295R
CTGCACTGGACTCCCAAAATACTG
12212012SXJ93rlv_QUEUE_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
G5
011-106518241F
CCAGTTTGACCCATACTACTGAGTCTCT
12212012SXJ93rlv_QUEUE_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
G6
011-106518241R
GGGATGACACATAGTGCCATCTG
12212012SXJ93rlv_QUEUE_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
H5
002-072399256F
CTCCCAAAGTGCTGGGATTACAGG
12212012SXJ93rlv_QUEUE_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
H6
002-072399256R
CCCTAATCCCATTTCCCACATCTG
12212012SXJ93rlv_QUEUE_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
A7
006-136890071F
GAAGAACCACCTACCCCCAG
12212012SXJ93rlv_QUEUE_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
A8
006-136890071R
CTACAGAATGCAGATTTCCCCCAC
12212012SXJ93rlv_QUEUE_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
B7
004-152360042F
CACCTCAGCCTCCGGAATTTTG
12212012SXJ93rlv_QUEUE_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
B8
004-152360042R
ACTCCTTTAGTCCCCACAACAACC
12212012SXJ93rlv_QUEUE_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
C7
00Y-005558821F
GCAGGCTTACTTGAAGTCCCAG
12212012SXJ93rlv_QUEUE_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
C8
00Y-005558821R
CGTGCCTACTAAGAACTGCTCTC
12212012SXJ93rlv_QUEUE_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
D7
014-050186827F
CCTGTGTAGCTGGGATTACAGGC
12212012SXJ93rlv_QUEUE_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
D8
014-050186827R
AAGATGGAGGCCAGACTGGG
12212012SXJ93rlv_QUEUE_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
E7
014-036739807F
GAGGGATCCAGGATGACAAAGG
12212012SXJ93rlv_QUEUE_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
E8
014-036739807R
CTGTCTATTCCATCTCAGGGGTTC
12212012SXJ93rlv_QUEUE_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
F7
012-089421427F
GCATTCACTCGCCTGTTCCTC
12212012SXJ93rlv_QUEUE_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
F8
012-089421427R
GCTGACTTAAGAGGGCTCTTTGC
12212012SXJ93rlv_QUEUE_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
G7
007-052877054F
GCAACTTAGGGCACGTGACC
12212012SXJ93rlv_QUEUE_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
G8
007-052877054R
TCCCCTTGCAACAGCACATGTTAG
12212012SXJ93rlv_QUEUE_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
H7
001-055972723F
ACCCCCAAATACCCTTTTCCCC
12212012SXJ93rlv_QUEUE_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
H8
001-055972723R
GGGTGTCGGCCTGAATTACATTG
12212012SXJ93rlv_QUEUE_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
A9
013-064006601F
CAGCATCCCGAGTAACCAGG
12212012SXJ93rlv_QUEUE_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
A10
013-064006601R
GAGTTCCAGTTACTCTTCCTCTCC
12212012SXJ93rlv_QUEUE_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
B9
004-035914090F
GCCAGAAGCTGCCACTATGC
12212012SXJ93rlv_QUEUE_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
B10
004-035914090R
GCACCTACATGCAGTGCACAG
12212012SXJ93rlv_QUEUE_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
C9
002-100505280F
GGGATGAACAATGACGGCAACAAG
12212012SXJ93rlv_QUEUE_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
C10
002-100505280R
CTGGACAGCACAGTGAGACC
12212012SXJ93rlv_QUEUE_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
D9
011-029003196F
GCCATTGCACATACAGACAGTGAG
12212012SXJ93rlv_QUEUE_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
D10
011-029003196R
GTAGCACACCTAAGAATCTAGCCC
12212012SXJ93rlv_QUEUE_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
E9
018-032835074F
GGGGATGGAGACAACTCCTTC
12212012SXJ93rlv_QUEUE_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
E10
018-032835074R
TAGCTTCTCTGAGCCTCAGTTTCC
12212012SXJ93rlv_QUEUE_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
F9
005-024257673F
CACTTCAGCCTTTCAAAGTGCTGG
12212012SXJ93rlv_QUEUE_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
F10
005-024257673R
GAGATACTGGTTCCCCCTCC
12212012SXJ93rlv_QUEUE_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
G9
00X-103046628F
GGGTTGCATCAGAAATGTCTGGAG
12212012SXJ93rlv_QUEUE_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
G10
00X-103046628R
CCTTCTATGCAGCCTGGAAGC
12212012SXJ93rlv_QUEUE_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
H9
001-231235899F
CTTGAGGCCAGTGCTTGTTTAGG
12212012SXJ93rlv_QUEUE_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
H10
001-231235899R
TCCCCCTCTTGGCCAAAAGC
12212012SXJ93rlv_QUEUE_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
A11
012-042468830F
GAATGGACACCTACCTTGGCC
12212012SXJ93rlv_QUEUE_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
A12
012-042468830R
GTCTGGCAATGGATCAGCTGCA
12212012SXJ93rlv_QUEUE_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
B11
011-076680948F
CCTGCATATTGTGCACATGTACCC
12212012SXJ93rlv_QUEUE_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
B12
011-076680948R
GGATGAACCCCTCTTGGCCA
12212012SXJ93rlv_QUEUE_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
C11
012-010998876F
CCAAGAAGATCTATAGGTCTCCGTGAAATG
12212012SXJ93rlv_QUEUE_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
C12
012-010998876R
GGTCCAGTGTCCCTGCTAAATG
12212012SXJ93rlv_QUEUE_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
D11
001-042172353F
GTAGGAGGGGACCAGGAAAC
12212012SXJ93rlv_QUEUE_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
D12
001-042172353R
CTGGCTAAAACTCACTGGTCAGCT
12212012SXJ93rlv_QUEUE_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
E11
003-033670533F
GGTGCCTCAACGATGATCAGTG
12212012SXJ93rlv_QUEUE_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
E12
003-033670533R
GTCCTTCTTCAAGGGCCAGC
12212012SXJ93rlv_QUEUE_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
F11
002-173274458F
CCCTATGAATCCTAGTGTGTTGCG
12212012SXJ93rlv_QUEUE_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
F12
002-173274458R
CAGGAGTTCAAGGTTACAGTGAGC
12212012SXJ93rlv_QUEUE_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
G11
003-159570943F
AGAAAACAGCAGCTGCCCGG
12212012SXJ93rlv_QUEUE_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
G12
003-159570943R
AAAAGAGCTCTCCCCCCTTACC
12212012SXJ93rlv_QUEUE_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
H11
002-177354353F
TGAGCAGCAGCCCCTCTACT
12212012SXJ93rlv_QUEUE_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
H12
002-177354353R
AAGAGTGACTGCACCCAGGC
12212012SXJ93rlv_QUEUE_Ranking-RVDs_5T	Primer pair generated using optimal parameters.