PROGNOS Summary for 12212012SXJ93rlv_QUEUE_Ranking-RVDs

The hg19 genome was searched for the TALEN pair targeting:
5'- TTTGTGGGCAACATGCTGG NN..NN ATAAACTGCAAAAGGCTGA -3'

Using these RVDs:
Left:  01NG02NG03NN04NG05NN06NN07NN08HD09NI10NI11HD12NI13NG14NN15HD16NG17NN18NN
Right: 01HD02NI03NN04HD05HD06NG07NG08NG09NG10NN11HD12NG13NN14NG15NG16NG17NI18NG

Up to 6 mismatches were allowed in each nuclease half-site.

Allowed spacing distances were: 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30

Homodimers and heterodimers of the nucleases were included in the output

4684 total sites were located
Mismatch Types
Genomic Regions
  0-0: 1
  0-1: 1
  3-5: 5
  4-4: 2
  3-6: 21
  4-5: 13
  4-6: 121
  5-5: 91
  5-6: 1014
  6-6: 3415
  Exons: 120
  Promoters: 33
  Introns: 1825
  Intergenic: 2706


The top 48 sites are ranked below according to a weighting of the RVD base preferences.
For the full output, click here.

This output will be deleted from the server one week after it is generated.  Please save your results.

Primer pairs were designed using the 422 bp of sequence on either side of the cleavage site to melt close to 60 °C

For easy visualization of cleavage on gels:
The smallest fragment will be longer than 125 bp, which is also the minimum separation distance between uncleaved and cleaved products.
The two cleavage products will be separated by a minimum size of 75 bp
Rank: 1	Homology Score: 98	RVD Match Score: 2	Left Half-Site RVD Score: 4.024	Right Half-Site RVD Score: 3.881
	Genomic Region: Exon	Closest Gene: CCR5
Match Type: L14R
	           Left Nuclease
	         TTTGTGGGCAACATGCTGG
	5'-TTGGT TTTGTGGGCAACATGCTGG         TCATCCTCATCCTG         ATAAACTGCAAAAGGCTGA AGAGC-3'
	3'-AACCA AAACACCCGTTGTACGACC         AGTAGGAGTAGGAC         TATTTGACGTTTTCCGACT TCTCG-5'
	                                                            TATTTGACGTTTTCCGACT
	                                                                  Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 003-046414377
Forward Primer: 5'-GCACAGGGTGGAACAAGATGG-3' Tm: 59.378
Reverse Primer: 5'-GTGACCGTCCTGGCTTTTAAAGCA-3' Tm: 59.038
Expected PCR Product Length: 446
Expected CelI Cleavage Product Sizes: 177, 269
In Silico PCR
Genome Browser

Rank: 2	Homology Score: 85	RVD Match Score: 2.14	Left Half-Site RVD Score: 4.024	Right Half-Site RVD Score: 5.045
	Genomic Region: Exon	Closest Gene: CCR2
Match Type: L14R
	           Left Nuclease
	         TTTGTGGGCAACATGCTGG
	5'-TTGGT TTTGTGGGCAACATGCTGG         TCGTCCTCATCTTA         ATAAACTGCAAAAAGCTGA AGTGC-3'
	3'-AACCA AAACACCCGTTGTACGACC         AGCAGGAGTAGAAT         TATTTGACGTTTTTCGACT TCACG-5'
	                                                            TATTTGACGTTTTCCGACT
	                                                                  Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 003-046399009
Forward Primer: 5'-CATCCACAACATGCTGTCCACATC-3' Tm: 59.038
Reverse Primer: 5'-CAAAGACCCACTCATTTGCAGCAG-3' Tm: 59.038
Expected PCR Product Length: 336
Expected CelI Cleavage Product Sizes: 206, 130
In Silico PCR
Genome Browser

Rank: 3	Homology Score: 10	RVD Match Score: 2.376	Left Half-Site RVD Score: 5.159	Right Half-Site RVD Score: 5.814
	Genomic Region: Intron	Closest Gene: ARSB
Match Type: R11R
	          Right Nuclease
	         TCAGCCTTTTGCAGTTTAT
	5'-AACAC CCAGCCTTTTCCTAATAAT          TGCTGAGTCGC           ATAACAAGCAAAAGGCTTA ATCCT-3'
	3'-TTGTG GGTCGGAAAAGGATTATTA          ACGACTCAGCG           TATTGTTCGTTTTCCGAAT TAGGA-5'
	                                                            TATTTGACGTTTTCCGACT
	                                                                  Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 005-078100805
Forward Primer: 5'-TAGCAGGGACACAGCATCCG-3' Tm: 59.508
Reverse Primer: 5'-CTCGTTGGCTGACTTATGCTCTG-3' Tm: 59.148
Expected PCR Product Length: 343
Expected CelI Cleavage Product Sizes: 212, 131
In Silico PCR
Genome Browser

Rank: 4	Homology Score: 17	RVD Match Score: 2.385	Left Half-Site RVD Score: 5.561	Right Half-Site RVD Score: 5.682
	Genomic Region: Intergenic	Closest Gene: FLJ42709
Match Type: R12L
	          Right Nuclease
	         TCAGCCTTTTGCAGTTTAT
	5'-ATATT CCAGCCTTTTCTTATTTCT          ATTCCATCCCTG          TGAACATGTTGCCCACAAA GTAGG-3'
	3'-TATAA GGTCGGAAAAGAATAAAGA          TAAGGTAGGGAC          ACTTGTACAACGGGTGTTT CATCC-5'
	                                                            GGTCGTACAACGGGTGTTT
	                                                                   Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 005-091821506
Forward Primer: 5'-AGGACTGGCTCCTTGTGGTG-3' Tm: 59.508
Reverse Primer: 5'-GACCTGCTTCTCTGGTCTTTTCTG-3' Tm: 59.038
Expected PCR Product Length: 563
Expected CelI Cleavage Product Sizes: 126, 437
In Silico PCR
Genome Browser

Rank: 5	Homology Score: 5	RVD Match Score: 2.446	Left Half-Site RVD Score: 5.764	Right Half-Site RVD Score: 6.281
	Genomic Region: Intron	Closest Gene: DMXL1
Match Type: L26L
	           Left Nuclease
	         TTTGTGGGCAACATGCTGG
	5'-TAGGT CTTCTGGATAACATGCTGA   AGCTTCTACGTCATTCAGCACTTGCT   TCAGCATGTTTTCCAGAGG ATCTA-3'
	3'-ATCCA GAAGACCTATTGTACGACT   TCGAAGATGCAGTAAGTCGTGAACGA   AGTCGTACAAAAGGTCTCC TAGAT-5'
	                                                            GGTCGTACAACGGGTGTTT
	                                                                   Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 005-118490138
Forward Primer: 5'-GTCTAGATGGATCGAACTAGCCAC-3' Tm: 59.038
Reverse Primer: 5'-GCACTGACTGCTCTCTAACTACG-3' Tm: 59.148
Expected PCR Product Length: 390
Expected CelI Cleavage Product Sizes: 236, 154
In Silico PCR
Genome Browser

Rank: 6	Homology Score: 17	RVD Match Score: 2.469	Left Half-Site RVD Score: 6.281	Right Half-Site RVD Score: 5.783
	Genomic Region: Intron	Closest Gene: NCOA5
Match Type: L16R
	           Left Nuclease
	         TTTGTGGGCAACATGCTGG
	5'-AAAAT AATCTAGAAAACATGCTGG        AAATGGCAGAACCATT        ATAAACTACAAAAGGATGG GGTGA-3'
	3'-TTTTA TTAGATCTTTTGTACGACC        TTTACCGTCTTGGTAA        TATTTGATGTTTTCCTACC CCACT-5'
	                                                            TATTTGACGTTTTCCGACT
	                                                                  Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 020-044711346
Forward Primer: 5'-GTGTCCAAGTGGTGGTCTGAAAAC-3' Tm: 59.038
Reverse Primer: 5'-CCGCTCTTGTTATTCCTAGTCCTC-3' Tm: 59.038
Expected PCR Product Length: 419
Expected CelI Cleavage Product Sizes: 129, 290
In Silico PCR
Genome Browser

Rank: 7	Homology Score: 17	RVD Match Score: 2.477	Left Half-Site RVD Score: 6.106	Right Half-Site RVD Score: 6.023
	Genomic Region: Intron	Closest Gene: VEPH1
Match Type: R29L
	          Right Nuclease
	         TCAGCCTTTTGCAGTTTAT
	5'-TCCAG TCAAATTTTAGCTATTTAT CCATTGCTGTGGTCCTCTGACCCCTTGGG  CCAGCAGGTTGGCCACATA GGCAC-3'
	3'-AGGTC AGTTTAAAATCGATAAATA GGTAACGACACCAGGAGACTGGGGAACCC  GGTCGTCCAACCGGTGTAT CCGTG-5'
	                                                            GGTCGTACAACGGGTGTTT
	                                                                   Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 003-157066450
Forward Primer: 5'-AAGGAAGAGGGGGCTGCAGA-3' Tm: 59.508
Reverse Primer: 5'-GCTGAGTCCTTGTCTGGTTTTGG-3' Tm: 59.148
Expected PCR Product Length: 388
Expected CelI Cleavage Product Sizes: 153, 235
In Silico PCR
Genome Browser

Rank: 8	Homology Score: 2	RVD Match Score: 2.495	Left Half-Site RVD Score: 7.453	Right Half-Site RVD Score: 4.782
	Genomic Region: Intron	Closest Gene: RABGAP1
Match Type: R10R
	          Right Nuclease
	         TCAGCCTTTTGCAGTTTAT
	5'-CCCCT TCAGTTTTTTGTTATTAAT           AGCACTATAG           ATAAATAGTTAAAGGATGT AAACA-3'
	3'-GGGGA AGTCAAAAAACAATAATTA           TCGTGATATC           TATTTATCAATTTCCTACA TTTGT-5'
	                                                            TATTTGACGTTTTCCGACT
	                                                                  Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 009-125759999
Forward Primer: 5'-GGCTCTGTCCCAAAGGAATCAC-3' Tm: 59.258
Reverse Primer: 5'-GTCAGATTGTTCTGCACAAGTGGC-3' Tm: 59.038
Expected PCR Product Length: 341
Expected CelI Cleavage Product Sizes: 208, 133
In Silico PCR
Genome Browser

Rank: 9	Homology Score: 5	RVD Match Score: 2.513	Left Half-Site RVD Score: 6.204	Right Half-Site RVD Score: 6.058
	Genomic Region: Intron	Closest Gene: CHN1
Match Type: R27R
	          Right Nuclease
	         TCAGCCTTTTGCAGTTTAT
	5'-ATACC CCCGCCTTTTGGTGTTTAG  GCACAACTGACCAGCATTAACATTGAA   ATAGAGATTATAAGGCTGA CAACA-3'
	3'-TATGG GGGCGGAAAACCACAAATC  CGTGTTGACTGGTCGTAATTGTAACTT   TATCTCTAATATTCCGACT GTTGT-5'
	                                                            TATTTGACGTTTTCCGACT
	                                                                  Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 002-175789857
Forward Primer: 5'-GACTCCCTCTTGGCCAACAG-3' Tm: 59.508
Reverse Primer: 5'-CAGTTGCAAATTCCCCCACAAGG-3' Tm: 59.148
Expected PCR Product Length: 331
Expected CelI Cleavage Product Sizes: 127, 204
In Silico PCR
Genome Browser

Rank: 10	Homology Score: 2	RVD Match Score: 2.516	Left Half-Site RVD Score: 6.64	Right Half-Site RVD Score: 5.877
	Genomic Region: Intergenic	Closest Gene: MIR4778
Match Type: R17L
	          Right Nuclease
	         TCAGCCTTTTGCAGTTTAT
	5'-AATGC AAAGCCTTCTGCTGTTGAC       ACAATTGCACTTGGCAA        TTTGGATGTTGCTGACAAA GAGGG-3'
	3'-TTACG TTTCGGAAGACGACAACTG       TGTTAACGTGAACCGTT        AAACCTACAACGACTGTTT CTCCC-5'
	                                                            GGTCGTACAACGGGTGTTT
	                                                                   Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 002-066451889
Forward Primer: 5'-CATATAGGATCCTCGATCACCCTG-3' Tm: 59.038
Reverse Primer: 5'-TGGACGTACGCAAACCCTGG-3' Tm: 59.508
Expected PCR Product Length: 348
Expected CelI Cleavage Product Sizes: 212, 136
In Silico PCR
Genome Browser

Rank: 11	Homology Score: 17	RVD Match Score: 2.519	Left Half-Site RVD Score: 4.677	Right Half-Site RVD Score: 8.139
	Genomic Region: Intergenic	Closest Gene: AP1AR
Match Type: R18L
	          Right Nuclease
	         TCAGCCTTTTGCAGTTTAT
	5'-AATTT TCAGCTTTTTCCTGTTTAT       CGTGATACGAATATTCCA       ACAGCATGTTAAACAAAAG ACGTT-3'
	3'-TTAAA AGTCGAAAAAGGACAAATA       GCACTATGCTTATAAGGT       TGTCGTACAATTTGTTTTC TGCAA-5'
	                                                            GGTCGTACAACGGGTGTTT
	                                                                   Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 004-113138626
Forward Primer: 5'-GCCATCCAAATGTGGACAGTAGG-3' Tm: 59.148
Reverse Primer: 5'-GTAGGGGAAACCAATTACAGTGCC-3' Tm: 59.038
Expected PCR Product Length: 394
Expected CelI Cleavage Product Sizes: 147, 247
In Silico PCR
Genome Browser

Rank: 12	Homology Score: 8	RVD Match Score: 2.521	Left Half-Site RVD Score: 7.061	Right Half-Site RVD Score: 5.561
	Genomic Region: Intron	Closest Gene: MAP4K3
Match Type: L30R
	           Left Nuclease
	         TTTGTGGGCAACATGCTGG
	5'-TGGGT TTGGTGGAAAAAATACTGG GAACCCTCCTACTCTCAACCAACATGAGGA AAAAACAGTAAAAGACTGG CACAG-3'
	3'-ACCCA AACCACCTTTTTTATGACC CTTGGGAGGATGAGAGTTGGTTGTACTCCT TTTTTGTCATTTTCTGACC GTGTC-5'
	                                                            TATTTGACGTTTTCCGACT
	                                                                  Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 002-039561318
Forward Primer: 5'-CCCTCTGCATGATCTGGTTGCATT-3' Tm: 59.038
Reverse Primer: 5'-GTAGTATCAGTGGGTGGGTGC-3' Tm: 59.378
Expected PCR Product Length: 339
Expected CelI Cleavage Product Sizes: 214, 125
In Silico PCR
Genome Browser

Rank: 13	Homology Score: 17	RVD Match Score: 2.521	Left Half-Site RVD Score: 6.771	Right Half-Site RVD Score: 5.6
	Genomic Region: Intergenic	Closest Gene: MIR563
Match Type: R28R
	          Right Nuclease
	         TCAGCCTTTTGCAGTTTAT
	5'-GCATT CTAACCAATTGCAATTTAT  GAACCAATTTGTTTTCCAATTTGAATAA  ATAAACTGAAAAAGTTTGA GACAG-3'
	3'-CGTAA GATTGGTTAACGTTAAATA  CTTGGTTAAACAAAAGGTTAAACTTATT  TATTTGACTTTTTCAAACT CTGTC-5'
	                                                            TATTTGACGTTTTCCGACT
	                                                                  Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 003-015921847
Forward Primer: 5'-GCTCTAGAACTCTAGACTGTGGG-3' Tm: 59.148
Reverse Primer: 5'-CTCACGGCCAATCCTGTTTTATCC-3' Tm: 59.038
Expected PCR Product Length: 412
Expected CelI Cleavage Product Sizes: 165, 247
In Silico PCR
Genome Browser

Rank: 14	Homology Score: 8	RVD Match Score: 2.522	Left Half-Site RVD Score: 5.867	Right Half-Site RVD Score: 6.708
	Genomic Region: Intron	Closest Gene: TBC1D1
Match Type: L25R
	           Left Nuclease
	         TTTGTGGGCAACATGCTGG
	5'-TTTTG GTTGTGAGCAAAATGCAGA   CTTACTTTCAAAATTGAGTTTTAGT    ATAAACTCAAATAGGTTGG CAGAA-3'
	3'-AAAAC CAACACTCGTTTTACGTCT   GAATGAAAGTTTTAACTCAAAATCA    TATTTGAGTTTATCCAACC GTCTT-5'
	                                                            TATTTGACGTTTTCCGACT
	                                                                  Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 004-038011091
Forward Primer: 5'-GTTTCAGGAGCTCTTTCCTAAATCCTTGTG-3' Tm: 60.058
Reverse Primer: 5'-GACAGATTCAATAAGGGTGTGGGG-3' Tm: 59.038
Expected PCR Product Length: 420
Expected CelI Cleavage Product Sizes: 294, 126
In Silico PCR
Genome Browser

Rank: 15	Homology Score: 5	RVD Match Score: 2.522	Left Half-Site RVD Score: 6.322	Right Half-Site RVD Score: 6.025
	Genomic Region: Intergenic	Closest Gene: TBC1D3P5
Match Type: R13R
	          Right Nuclease
	         TCAGCCTTTTGCAGTTTAT
	5'-CACAT CCAGCCTATTATACTTTAT         AAAGATTTATATT          ATATACAGTAAAATGCTAT GGATT-3'
	3'-GTGTA GGTCGGATAATATGAAATA         TTTCTAAATATAA          TATATGTCATTTTACGATA CCTAA-5'
	                                                            TATTTGACGTTTTCCGACT
	                                                                  Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 017-025719104
Forward Primer: 5'-CATCTGTCCTTCAGGACTTGAGAG-3' Tm: 59.038
Reverse Primer: 5'-GGGATAGCCACTGGAAAAGGG-3' Tm: 59.378
Expected PCR Product Length: 337
Expected CelI Cleavage Product Sizes: 211, 126
In Silico PCR
Genome Browser

Rank: 16	Homology Score: 2	RVD Match Score: 2.525	Left Half-Site RVD Score: 6.985	Right Half-Site RVD Score: 5.439
	Genomic Region: Intergenic	Closest Gene: CHRDL1
Match Type: R30R
	          Right Nuclease
	         TCAGCCTTTTGCAGTTTAT
	5'-AAGAG TCAACTATGTGCTGTCTAT ATGAAACCCAGTTTAATATAAAAACACAGG TTAAAAAGTAAAAGGATGG AAGAT-3'
	3'-TTCTC AGTTGATACACGACAGATA TACTTTGGGTCAAATTATATTTTTGTGTCC AATTTTTCATTTTCCTACC TTCTA-5'
	                                                            TATTTGACGTTTTCCGACT
	                                                                  Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 00X-109896865
Forward Primer: 5'-CCATTGTACAGAGTGGTAACTCCAGTG-3' Tm: 60.438
Reverse Primer: 5'-GTTAGGGGGGAGTCCCTTCT-3' Tm: 59.508
Expected PCR Product Length: 433
Expected CelI Cleavage Product Sizes: 284, 149
In Silico PCR
Genome Browser

Rank: 17	Homology Score: 5	RVD Match Score: 2.525	Left Half-Site RVD Score: 7.275	Right Half-Site RVD Score: 5.194
	Genomic Region: Intron	Closest Gene: KCNJ15
Match Type: R18R
	          Right Nuclease
	         TCAGCCTTTTGCAGTTTAT
	5'-TAAGA TCACACTTCTCCAATTTCT       GTCCTCCTCTACTCCTCA       ATAAACAGGAAGATGCTGT CTGTG-3'
	3'-ATTCT AGTGTGAAGAGGTTAAAGA       CAGGAGGAGATGAGGAGT       TATTTGTCCTTCTACGACA GACAC-5'
	                                                            TATTTGACGTTTTCCGACT
	                                                                  Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 021-039646075
Forward Primer: 5'-CCTGGGCAAAGTCATGTTAGGTG-3' Tm: 59.148
Reverse Primer: 5'-CAAGGCATTTGCTCTCATGAGGC-3' Tm: 59.148
Expected PCR Product Length: 369
Expected CelI Cleavage Product Sizes: 239, 130
In Silico PCR
Genome Browser

Rank: 18	Homology Score: 2	RVD Match Score: 2.529	Left Half-Site RVD Score: 6.426	Right Half-Site RVD Score: 5.997
	Genomic Region: Intergenic	Closest Gene: RAB9BP1
Match Type: R23R
	          Right Nuclease
	         TCAGCCTTTTGCAGTTTAT
	5'-TGTAT TCAGCACATTACTGTTTAC    TTGAAACAGGTGACAGATGTATT     AGAAAGAGCATAAGGCTTC TGATT-3'
	3'-ACATA AGTCGTGTAATGACAAATG    AACTTTGTCCACTGTCTACATAA     TCTTTCTCGTATTCCGAAG ACTAA-5'
	                                                            TATTTGACGTTTTCCGACT
	                                                                  Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 005-105472401
Forward Primer: 5'-GCCTCTCTTTTCTAGGTGAAGACG-3' Tm: 59.038
Reverse Primer: 5'-GAGGAAGAGGGGGACTGACA-3' Tm: 59.508
Expected PCR Product Length: 675
Expected CelI Cleavage Product Sizes: 438, 237
In Silico PCR
Genome Browser

Rank: 19	Homology Score: 2	RVD Match Score: 2.536	Left Half-Site RVD Score: 6.343	Right Half-Site RVD Score: 6.368
	Genomic Region: Intron	Closest Gene: CNST
Match Type: R30L
	          Right Nuclease
	         TCAGCCTTTTGCAGTTTAT
	5'-TGTTT TCCATCTTTTGCTCTTTAC AAACTGGTTAAGACTGTTTCCCTCTCAGTG TCAGCAAGGTGCTTATAAA ACCTG-3'
	3'-ACAAA AGGTAGAAAACGAGAAATG TTTGACCAATTCTGACAAAGGGAGAGTCAC AGTCGTTCCACGAATATTT TGGAC-5'
	                                                            GGTCGTACAACGGGTGTTT
	                                                                   Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 001-246821429
Forward Primer: 5'-CAGCCAGATTCAAGATCCTCTCC-3' Tm: 59.148
Reverse Primer: 5'-GCTTCCCTGAATGTGGGTCTATAG-3' Tm: 59.038
Expected PCR Product Length: 394
Expected CelI Cleavage Product Sizes: 143, 251
In Silico PCR
Genome Browser

Rank: 20	Homology Score: 5	RVD Match Score: 2.538	Left Half-Site RVD Score: 7.14	Right Half-Site RVD Score: 5.422
	Genomic Region: Intron	Closest Gene: ANLN
Match Type: R29R
	          Right Nuclease
	         TCAGCCTTTTGCAGTTTAT
	5'-TTCTG TCAGTCTTATGATCTCTAT TTAACATTAATGCTCATCAGTTATTGTGT  TTAAACAGCAAAAGGAGGG GTATA-3'
	3'-AAGAC AGTCAGAATACTAGAGATA AATTGTAATTACGAGTAGTCAATAACACA  AATTTGTCGTTTTCCTCCC CATAT-5'
	                                                            TATTTGACGTTTTCCGACT
	                                                                  Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 007-036433669
Forward Primer: 5'-ATCCTTTTGCTTCAGCCACCCC-3' Tm: 59.258
Reverse Primer: 5'-AGATCCCCTCTTGATGAAGGGGAC-3' Tm: 60.908
Expected PCR Product Length: 326
Expected CelI Cleavage Product Sizes: 200, 126
In Silico PCR
Genome Browser

Rank: 21	Homology Score: 10	RVD Match Score: 2.539	Left Half-Site RVD Score: 6.175	Right Half-Site RVD Score: 6.577
	Genomic Region: Intergenic	Closest Gene: LINC00353
Match Type: R13L
	          Right Nuclease
	         TCAGCCTTTTGCAGTTTAT
	5'-AGATT TAACCATTTTGCTATATAT         GTATATATTTCAA          ACAGCATGTTGCACCCAAT AAATG-3'
	3'-TCTAA ATTGGTAAAACGATATATA         CATATATAAAGTT          TGTCGTACAACGTGGGTTA TTTAC-5'
	                                                            GGTCGTACAACGGGTGTTT
	                                                                   Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 013-089954123
Forward Primer: 5'-GAGTGTGTTGGGGAGATATTGGTC-3' Tm: 59.038
Reverse Primer: 5'-CTAGTGACTTCAGTGTGTATGCATGAAGC-3' Tm: 60.178
Expected PCR Product Length: 558
Expected CelI Cleavage Product Sizes: 224, 334
In Silico PCR
Genome Browser

Rank: 22	Homology Score: 10	RVD Match Score: 2.539	Left Half-Site RVD Score: 6.197	Right Half-Site RVD Score: 6.554
	Genomic Region: Intron	Closest Gene: MLANA
Match Type: R21L
	          Right Nuclease
	         TCAGCCTTTTGCAGTTTAT
	5'-CTTTT ATATCCTTTTCCAGTTTAT     TGCATTAGTTATTTGCAACTT      CCAGTATGATGCTGAAAAG GAGTG-3'
	3'-GAAAA TATAGGAAAAGGTCAAATA     ACGTAATCAATAAACGTTGAA      GGTCATACTACGACTTTTC CTCAC-5'
	                                                            GGTCGTACAACGGGTGTTT
	                                                                   Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 009-005901106
Forward Primer: 5'-CTTAACCTATGGTGAGTGAGAGGC-3' Tm: 59.038
Reverse Primer: 5'-GGTGACAGAATGAGACCCCATC-3' Tm: 59.258
Expected PCR Product Length: 456
Expected CelI Cleavage Product Sizes: 133, 323
In Silico PCR
Genome Browser

Rank: 23	Homology Score: 10	RVD Match Score: 2.544	Left Half-Site RVD Score: 6.909	Right Half-Site RVD Score: 5.909
	Genomic Region: Intergenic	Closest Gene: EN1
Match Type: L21R
	           Left Nuclease
	         TTTGTGGGCAACATGCTGG
	5'-AGAAG AGTGTAGCCAAAATCCTGG     GAACAAACCGGGAATATCTGA      AGAGACAGCAAAAGCCTGA GGCAG-3'
	3'-TCTTC TCACATCGGTTTTAGGACC     CTTGTTTGGCCCTTATAGACT      TCTCTGTCGTTTTCGGACT CCGTC-5'
	                                                            TATTTGACGTTTTCCGACT
	                                                                  Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 002-119344377
Forward Primer: 5'-GTGGGTTAGAAGGTGGGAACAG-3' Tm: 59.258
Reverse Primer: 5'-GAACTAGATGCCCATGTGGGTC-3' Tm: 59.258
Expected PCR Product Length: 337
Expected CelI Cleavage Product Sizes: 207, 130
In Silico PCR
Genome Browser

Rank: 24	Homology Score: 5	RVD Match Score: 2.547	Left Half-Site RVD Score: 6.382	Right Half-Site RVD Score: 6.444
	Genomic Region: Intergenic	Closest Gene: LINC00470
Match Type: R27L
	          Right Nuclease
	         TCAGCCTTTTGCAGTTTAT
	5'-TAAGG ACAGACATTTTCAATTTAT  TTGTGTCTTTTTAAACTCAGTTTAAAT   ACAGTGTGTTGCACATAAC AAAAC-3'
	3'-ATTCC TGTCTGTAAAAGTTAAATA  AACACAGAAAAATTTGAGTCAAATTTA   TGTCACACAACGTGTATTG TTTTG-5'
	                                                            GGTCGTACAACGGGTGTTT
	                                                                   Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 018-001712330
Forward Primer: 5'-CAGGGACATTAACTGTTTGGAGGG-3' Tm: 59.038
Reverse Primer: 5'-CCTAGGCTTGTCAATAGACAGGAG-3' Tm: 59.038
Expected PCR Product Length: 474
Expected CelI Cleavage Product Sizes: 344, 130
In Silico PCR
Genome Browser

Rank: 25	Homology Score: 10	RVD Match Score: 2.548	Left Half-Site RVD Score: 6.598	Right Half-Site RVD Score: 6.237
	Genomic Region: Intergenic	Closest Gene: CDH8
Match Type: R10L
	          Right Nuclease
	         TCAGCCTTTTGCAGTTTAT
	5'-TATCC CCACCCTTTTGGAGTTTAG           GCACAACTGA           CCAGCATTTTGGTTAAAAT AGAGA-3'
	3'-ATAGG GGTGGGAAAACCTCAAATC           CGTGTTGACT           GGTCGTAAAACCAATTTTA TCTCT-5'
	                                                            GGTCGTACAACGGGTGTTT
	                                                                   Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 016-062697711
Forward Primer: 5'-ACTGAATGGACCCCTCGTGG-3' Tm: 59.508
Reverse Primer: 5'-GGCTTTGCAGGGCCATTTCAAAC-3' Tm: 59.148
Expected PCR Product Length: 446
Expected CelI Cleavage Product Sizes: 133, 313
In Silico PCR
Genome Browser

Rank: 26	Homology Score: 2	RVD Match Score: 2.55	Left Half-Site RVD Score: 6.111	Right Half-Site RVD Score: 6.754
	Genomic Region: Intergenic	Closest Gene: ZNF436
Match Type: R13L
	          Right Nuclease
	         TCAGCCTTTTGCAGTTTAT
	5'-ACTCT GCAGCCTTTTCCTTTTAAG         CCAGGTTCTCACT          CTAGTCTGTTGCCCAGACT GGAGT-3'
	3'-TGAGA CGTCGGAAAAGGAAAATTC         GGTCCAAGAGTGA          GATCAGACAACGGGTCTGA CCTCA-5'
	                                                            GGTCGTACAACGGGTGTTT
	                                                                   Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 001-023678545
Forward Primer: 5'-GAGTGCTGAACACTCAGTCACAAC-3' Tm: 59.038
Reverse Primer: 5'-GATCTTCCCACCTCAGCCTC-3' Tm: 59.508
Expected PCR Product Length: 342
Expected CelI Cleavage Product Sizes: 133, 209
In Silico PCR
Genome Browser

Rank: 27	Homology Score: 5	RVD Match Score: 2.553	Left Half-Site RVD Score: 5.992	Right Half-Site RVD Score: 6.916
	Genomic Region: Intron	Closest Gene: ZNF280D
Match Type: R16L
	          Right Nuclease
	         TCAGCCTTTTGCAGTTTAT
	5'-TGTGC CCAGCCTGTTTCTGTGTAT        TTAAAAAATTTTTTTT        CTAGAATGTTAGTCAGAAA AATAT-3'
	3'-ACACG GGTCGGACAAAGACACATA        AATTTTTTAAAAAAAA        GATCTTACAATCAGTCTTT TTATA-5'
	                                                            GGTCGTACAACGGGTGTTT
	                                                                   Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 015-056932411
Forward Primer: 5'-CAAGCCATATTGAAGCCTGAGGCA-3' Tm: 59.038
Reverse Primer: 5'-GCATTTGCTTCCATTAGCCAGGAG-3' Tm: 59.038
Expected PCR Product Length: 373
Expected CelI Cleavage Product Sizes: 233, 140
In Silico PCR
Genome Browser

Rank: 28	Homology Score: 5	RVD Match Score: 2.554	Left Half-Site RVD Score: 5.981	Right Half-Site RVD Score: 6.69
	Genomic Region: Intergenic	Closest Gene: LINC00184
Match Type: R17R
	          Right Nuclease
	         TCAGCCTTTTGCAGTTTAT
	5'-CTGGG TCAGTCTTTTAATTTTTAT       GAGTTAGTATGCCTCTT        GTAGACAGCATAAAGTTGA ATTTT-3'
	3'-GACCC AGTCAGAAAATTAAAAATA       CTCAATCATACGGAGAA        CATCTGTCGTATTTCAACT TAAAA-5'
	                                                            TATTTGACGTTTTCCGACT
	                                                                  Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 001-234812284
Forward Primer: 5'-GCCTGCCTGCTGAAGACATAAG-3' Tm: 59.258
Reverse Primer: 5'-CGCCTGACTTTATTGAATGCAGCC-3' Tm: 59.038
Expected PCR Product Length: 486
Expected CelI Cleavage Product Sizes: 350, 136
In Silico PCR
Genome Browser

Rank: 29	Homology Score: 5	RVD Match Score: 2.555	Left Half-Site RVD Score: 5.499	Right Half-Site RVD Score: 7.734
	Genomic Region: Intergenic	Closest Gene: LOC340107
Match Type: L21L
	           Left Nuclease
	         TTTGTGGGCAACATGCTGG
	5'-GAATG ATTGTGACCAAAATGCTGA     TAGTGACAGGTACAGTGAAGT      CCAGGATGAGGTGCTCAAA GGGAA-3'
	3'-CTTAC TAACACTGGTTTTACGACT     ATCACTGTCCATGTCACTTCA      GGTCCTACTCCACGAGTTT CCCTT-5'
	                                                            GGTCGTACAACGGGTGTTT
	                                                                   Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 005-025770689
Forward Primer: 5'-GGAGGGAGGTATTGCCATGAAG-3' Tm: 59.258
Reverse Primer: 5'-CTTCTACCACATAGTTGGGCTGC-3' Tm: 59.148
Expected PCR Product Length: 510
Expected CelI Cleavage Product Sizes: 177, 333
In Silico PCR
Genome Browser

Rank: 30	Homology Score: 2	RVD Match Score: 2.564	Left Half-Site RVD Score: 6.941	Right Half-Site RVD Score: 5.842
	Genomic Region: Intergenic	Closest Gene: LARGE
Match Type: R30R
	          Right Nuclease
	         TCAGCCTTTTGCAGTTTAT
	5'-ATTTC TCATCCCTTTATTGTGTAT ATTTGAGGTTTACAACATGATGTTATAAGT ATAGATAGTAAAATGCTTA CTACA-3'
	3'-TAAAG AGTAGGGAAATAACACATA TAAACTCCAAATGTTGTACTACAATATTCA TATCTATCATTTTACGAAT GATGT-5'
	                                                            TATTTGACGTTTTCCGACT
	                                                                  Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 022-034808553
Forward Primer: 5'-CATGAGGAAGTGTCAGACAAACCC-3' Tm: 59.038
Reverse Primer: 5'-GCGGCAGAGGATCAGGAAAG-3' Tm: 59.508
Expected PCR Product Length: 669
Expected CelI Cleavage Product Sizes: 408, 261
In Silico PCR
Genome Browser

Rank: 31	Homology Score: 2	RVD Match Score: 2.566	Left Half-Site RVD Score: 7.762	Right Half-Site RVD Score: 5.58
	Genomic Region: Intron	Closest Gene: NRG4
Match Type: L30L
	           Left Nuclease
	         TTTGTGGGCAACATGCTGG
	5'-TGGAT GTGGTGGGCAAAAAGATGA TTCACATCCTGAGTAGGTCAGCGGGAGATT TCATCATGTTGCTTAGAAG GGTGC-3'
	3'-ACCTA CACCACCCGTTTTTCTACT AAGTGTAGGACTCATCCAGTCGCCCTCTAA AGTAGTACAACGAATCTTC CCACG-5'
	                                                            GGTCGTACAACGGGTGTTT
	                                                                   Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 015-076238324
Forward Primer: 5'-CTCCTGCACTTTGGGGCCAT-3' Tm: 59.508
Reverse Primer: 5'-GACAGAGCGAGACCTCGTCTCA-3' Tm: 61.288
Expected PCR Product Length: 415
Expected CelI Cleavage Product Sizes: 170, 245
In Silico PCR
Genome Browser

Rank: 32	Homology Score: 5	RVD Match Score: 2.567	Left Half-Site RVD Score: 7.503	Right Half-Site RVD Score: 5.573
	Genomic Region: Intergenic	Closest Gene: C3orf80
Match Type: R27L
	          Right Nuclease
	         TCAGCCTTTTGCAGTTTAT
	5'-AGCTT CCAGCCATTTGAAATGTAA  TGATTACTCCTTAGATTGTTTATTTTC   TTAGCATGTTCTTCACAAA CCTAA-3'
	3'-TCGAA GGTCGGTAAACTTTACATT  ACTAATGAGGAATCTAACAAATAAAAG   AATCGTACAAGAAGTGTTT GGATT-5'
	                                                            GGTCGTACAACGGGTGTTT
	                                                                   Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 003-159856803
Forward Primer: 5'-CCTTGGCGACAGAGCAAGAC-3' Tm: 59.508
Reverse Primer: 5'-CACGCACACACACACACGATAAG-3' Tm: 59.148
Expected PCR Product Length: 427
Expected CelI Cleavage Product Sizes: 253, 174
In Silico PCR
Genome Browser

Rank: 33	Homology Score: 5	RVD Match Score: 2.567	Left Half-Site RVD Score: 6.241	Right Half-Site RVD Score: 6.56
	Genomic Region: Intron	Closest Gene: SLC39A11
Match Type: R12R
	          Right Nuclease
	         TCAGCCTTTTGCAGTTTAT
	5'-CAAAT CCAGCCTTCTGTTCCTTAT          TAGGCACTTTTA          GTAACAGGCAAAAGGTTGA CATGG-3'
	3'-GTTTA GGTCGGAAGACAAGGAATA          ATCCGTGAAAAT          CATTGTCCGTTTTCCAACT GTACC-5'
	                                                            TATTTGACGTTTTCCGACT
	                                                                  Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 017-070934484
Forward Primer: 5'-AAAGCCCAGTTCCACATCTTCCTC-3' Tm: 59.038
Reverse Primer: 5'-GGTAGCAGCAGCCTTCATTAGGA-3' Tm: 59.148
Expected PCR Product Length: 376
Expected CelI Cleavage Product Sizes: 126, 250
In Silico PCR
Genome Browser

Rank: 34	Homology Score: 5	RVD Match Score: 2.569	Left Half-Site RVD Score: 8.063	Right Half-Site RVD Score: 4.943
	Genomic Region: Intergenic	Closest Gene: PVRL3-AS1
Match Type: R25R
	          Right Nuclease
	         TCAGCCTTTTGCAGTTTAT
	5'-GGTTG TAAGACTTCTACATTTTAC   TTTAAATGATAAAATATTAACTCAA    ATAAACAGTGAAATGTTGA GTAGA-3'
	3'-CCAAC ATTCTGAAGATGTAAAATG   AAATTTACTATTTTATAATTGAGTT    TATTTGTCACTTTACAACT CATCT-5'
	                                                            TATTTGACGTTTTCCGACT
	                                                                  Right Nuclease
Warning! Primer pair was generated using suboptimal parameters. 
Primer Index: 003-110537278
Forward Primer: 5'-GTGGGTCTCTCTTCACTTTATTGATTGTTTCC-3' Tm: 59.848
Reverse Primer: 5'-GGTAAGAAGAGCGAAACTCCGTC-3' Tm: 59.148
Expected PCR Product Length: 598
Expected CelI Cleavage Product Sizes: 237, 361
In Silico PCR
Genome Browser

Rank: 35	Homology Score: 13	RVD Match Score: 2.57	Left Half-Site RVD Score: 5.706	Right Half-Site RVD Score: 7.391
	Genomic Region: Intergenic	Closest Gene: AOAH
Match Type: L24R
	           Left Nuclease
	         TTTGTGGGCAACATGCTGG
	5'-GTCCT CTTGTAGACAACATGCATG    CCTCCCTGCTCCCATTTGCTATCT    TTAATCTGCTAAAGGCTAA AAACC-3'
	3'-CAGGA GAACATCTGTTGTACGTAC    GGAGGGACGAGGGTAAACGATAGA    AATTAGACGATTTCCGATT TTTGG-5'
	                                                            TATTTGACGTTTTCCGACT
	                                                                  Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 007-036783522
Forward Primer: 5'-GGTTGTTTCTCTTCACTCACTGGC-3' Tm: 59.038
Reverse Primer: 5'-GCCCAACAATGCTGAACACAGATG-3' Tm: 59.038
Expected PCR Product Length: 352
Expected CelI Cleavage Product Sizes: 129, 223
In Silico PCR
Genome Browser

Rank: 36	Homology Score: 5	RVD Match Score: 2.571	Left Half-Site RVD Score: 6.783	Right Half-Site RVD Score: 6.294
	Genomic Region: Intron	Closest Gene: NRXN3
Match Type: L20R
	           Left Nuclease
	         TTTGTGGGCAACATGCTGG
	5'-GCTGG TTTAGGAGCAACACTCTGG      GAATCATTGTGTTAGGAACT      AAAAACAGGAGAGGGCTGT AGCAC-3'
	3'-CGACC AAATCCTCGTTGTGAGACC      CTTAGTAACACAATCCTTGA      TTTTTGTCCTCTCCCGACA TCGTG-5'
	                                                            TATTTGACGTTTTCCGACT
	                                                                  Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 014-079451010
Forward Primer: 5'-ACTCAGATTCCTCAATTCTGCCCC-3' Tm: 59.038
Reverse Primer: 5'-GGGCAAAGGCCATGTAGAATCAG-3' Tm: 59.148
Expected PCR Product Length: 368
Expected CelI Cleavage Product Sizes: 126, 242
In Silico PCR
Genome Browser

Rank: 37	Homology Score: 5	RVD Match Score: 2.573	Left Half-Site RVD Score: 5.932	Right Half-Site RVD Score: 6.941
	Genomic Region: Intergenic	Closest Gene: TECRL
Match Type: R10R
	          Right Nuclease
	         TCAGCCTTTTGCAGTTTAT
	5'-TGGCA ACAGCCTTTTCATTTTTAA           TATCTTCCTG           TCAAATTGCAAAATGTTGA TGAGA-3'
	3'-ACCGT TGTCGGAAAAGTAAAAATT           ATAGAAGGAC           AGTTTAACGTTTTACAACT ACTCT-5'
	                                                            TATTTGACGTTTTCCGACT
	                                                                  Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 004-065322559
Forward Primer: 5'-GAGCTGATGGACCTGAAGGTAC-3' Tm: 59.258
Reverse Primer: 5'-GCCACTCCATATGGCCATAAGTAG-3' Tm: 59.038
Expected PCR Product Length: 396
Expected CelI Cleavage Product Sizes: 254, 142
In Silico PCR
Genome Browser

Rank: 38	Homology Score: 10	RVD Match Score: 2.573	Left Half-Site RVD Score: 7.191	Right Half-Site RVD Score: 6.156
	Genomic Region: Intron	Closest Gene: RIMS2
Match Type: L24L
	           Left Nuclease
	         TTTGTGGGCAACATGCTGG
	5'-ATGGA TATCTCTTCAACATACTGG    AGTCTTTAGGGTTTACTGTAGATA    AGATCATGTTGTCCACAAA CAGAG-3'
	3'-TACCT ATAGAGAAGTTGTATGACC    TCAGAAATCCCAAATGACATCTAT    TCTAGTACAACAGGTGTTT GTCTC-5'
	                                                            GGTCGTACAACGGGTGTTT
	                                                                   Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 008-104699899
Forward Primer: 5'-CATACCCCAGTCTCTGGTGATC-3' Tm: 59.258
Reverse Primer: 5'-CTCAACGGTGAAAAGTTGGAAACTTTCCTC-3' Tm: 60.058
Expected PCR Product Length: 542
Expected CelI Cleavage Product Sizes: 345, 197
In Silico PCR
Genome Browser

Rank: 39	Homology Score: 5	RVD Match Score: 2.574	Left Half-Site RVD Score: 7.638	Right Half-Site RVD Score: 5.564
	Genomic Region: Intergenic	Closest Gene: PXDNL
Match Type: L20R
	           Left Nuclease
	         TTTGTGGGCAACATGCTGG
	5'-GGCTA TGTGTAGGAAACATACTTT      AGATTTAAAGAAACAAATAG      ATTAATGGTAAAAGGATGA AGAAA-3'
	3'-CCGAT ACACATCCTTTGTATGAAA      TCTAAATTTCTTTGTTTATC      TAATTACCATTTTCCTACT TCTTT-5'
	                                                            TATTTGACGTTTTCCGACT
	                                                                  Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 008-051977566
Forward Primer: 5'-CAGAGATCGCACCATTGCATTCC-3' Tm: 59.148
Reverse Primer: 5'-GTCTAAGAATCTGTGGGCAAATGTATGGTC-3' Tm: 60.058
Expected PCR Product Length: 341
Expected CelI Cleavage Product Sizes: 126, 215
In Silico PCR
Genome Browser

Rank: 40	Homology Score: 5	RVD Match Score: 2.575	Left Half-Site RVD Score: 6.63	Right Half-Site RVD Score: 6.472
	Genomic Region: Intergenic	Closest Gene: FAM41AY1
Match Type: R21L
	          Right Nuclease
	         TCAGCCTTTTGCAGTTTAT
	5'-GGTCT GCAGCTCTCTGCTGTTTCT     GAGTCATACAAGGCTTCTGGG      CCAGTTTGGTGCCCACAGT GGGTC-3'
	3'-CCAGA CGTCGAGAGACGACAAAGA     CTCAGTATGTTCCGAAGACCC      GGTCAAACCACGGGTGTCA CCCAG-5'
	                                                            GGTCGTACAACGGGTGTTT
	                                                                   Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 00Y-019313071
Forward Primer: 5'-GTGGGAGCCGTGTCCATTGA-3' Tm: 59.508
Reverse Primer: 5'-GCTACCAAAGACTCTTGCAAAGGG-3' Tm: 59.038
Expected PCR Product Length: 383
Expected CelI Cleavage Product Sizes: 149, 234
In Silico PCR
Genome Browser

Rank: 41	Homology Score: 5	RVD Match Score: 2.576	Left Half-Site RVD Score: 6.246	Right Half-Site RVD Score: 6.646
	Genomic Region: Intergenic	Closest Gene: PCDH20
Match Type: R27R
	          Right Nuclease
	         TCAGCCTTTTGCAGTTTAT
	5'-ATTAG ACAACTTTTTTTTGTTTAT  ATATTTCTGAAGACTTCTGCTCATAGA   ATATAATGTAAAAGACTGC TGAGA-3'
	3'-TAATC TGTTGAAAAAAAACAAATA  TATAAAGACTTCTGAAGACGAGTATCT   TATATTACATTTTCTGACG ACTCT-5'
	                                                            TATTTGACGTTTTCCGACT
	                                                                  Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 013-061919703
Forward Primer: 5'-CCTGGTCTGTTCTAGTGGCTC-3' Tm: 59.378
Reverse Primer: 5'-GAGAAGTTCAACAGTTGAGGCAATCTGTG-3' Tm: 60.178
Expected PCR Product Length: 630
Expected CelI Cleavage Product Sizes: 451, 179
In Silico PCR
Genome Browser

Rank: 42	Homology Score: 2	RVD Match Score: 2.578	Left Half-Site RVD Score: 7.356	Right Half-Site RVD Score: 5.808
	Genomic Region: Intron	Closest Gene: DMD
Match Type: R19L
	          Right Nuclease
	         TCAGCCTTTTGCAGTTTAT
	5'-AAAGG ACAGCGTTTGTCTGTTTTT      GGTTATCCTTGTGCCTATA       TTAGTATGTGGCACACAAT AGTCA-3'
	3'-TTTCC TGTCGCAAACAGACAAAAA      CCAATAGGAACACGGATAT       AATCATACACCGTGTGTTA TCAGT-5'
	                                                            GGTCGTACAACGGGTGTTT
	                                                                   Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 00X-032184490
Forward Primer: 5'-TCTGAGCCCAGGGAGGTCAA-3' Tm: 59.508
Reverse Primer: 5'-CACAGAAGCAGCAGGAGCAAG-3' Tm: 59.378
Expected PCR Product Length: 483
Expected CelI Cleavage Product Sizes: 280, 203
In Silico PCR
Genome Browser

Rank: 43	Homology Score: 8	RVD Match Score: 2.578	Left Half-Site RVD Score: 6.363	Right Half-Site RVD Score: 6.777
	Genomic Region: Exon	Closest Gene: PPM1E
Match Type: R18L
	          Right Nuclease
	         TCAGCCTTTTGCAGTTTAT
	5'-AACTG TCTGCTTTTTGCTATTTCT       GCAGTTTCAAGTTAGTTA       GAAGCATGTTGTCAACTAA AGACA-3'
	3'-TTGAC AGACGAAAAACGATAAAGA       CGTCAAAGTTCAATCAAT       CTTCGTACAACAGTTGATT TCTGT-5'
	                                                            GGTCGTACAACGGGTGTTT
	                                                                   Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 017-057059452
Forward Primer: 5'-GTCTAAAGTGAGCCCAGAGGCC-3' Tm: 61.288
Reverse Primer: 5'-CTGCTGTTGACTAGGGGAGG-3' Tm: 59.508
Expected PCR Product Length: 325
Expected CelI Cleavage Product Sizes: 200, 125
In Silico PCR
Genome Browser

Rank: 44	Homology Score: 8	RVD Match Score: 2.578	Left Half-Site RVD Score: 7.717	Right Half-Site RVD Score: 5.499
	Genomic Region: Exon	Closest Gene: VAMP3
Match Type: R12L
	          Right Nuclease
	         TCAGCCTTTTGCAGTTTAT
	5'-TCTAC CCAGCAGTTGGCAGATTAT          CAGCTGTGGACT          CCAGCATGTTTCTGATAAT TATGC-3'
	3'-AGATG GGTCGTCAACCGTCTAATA          GTCGACACCTGA          GGTCGTACAAAGACTATTA ATACG-5'
	                                                            GGTCGTACAACGGGTGTTT
	                                                                   Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 001-007840539
Forward Primer: 5'-AGGCTGTAGACAGTGTGGCTC-3' Tm: 59.378
Reverse Primer: 5'-CATTCACCCCTGACCAATGCC-3' Tm: 59.378
Expected PCR Product Length: 329
Expected CelI Cleavage Product Sizes: 126, 203
In Silico PCR
Genome Browser

Rank: 45	Homology Score: 2	RVD Match Score: 2.58	Left Half-Site RVD Score: 6.565	Right Half-Site RVD Score: 6.59
	Genomic Region: Intron	Closest Gene: UNC80
Match Type: R10L
	          Right Nuclease
	         TCAGCCTTTTGCAGTTTAT
	5'-CAGTG ACATTCTTAAGCTGTTTAT           CTGATAGAAG           ACAGTAGGTTGCTCTTAAA CACAT-3'
	3'-GTCAC TGTAAGAATTCGACAAATA           GACTATCTTC           TGTCATCCAACGAGAATTT GTGTA-5'
	                                                            GGTCGTACAACGGGTGTTT
	                                                                   Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 002-210791640
Forward Primer: 5'-CATCTGTGCAGCAGTACTTCCC-3' Tm: 59.258
Reverse Primer: 5'-TTCTCTGGTCACGAACAATGCTCC-3' Tm: 59.038
Expected PCR Product Length: 329
Expected CelI Cleavage Product Sizes: 127, 202
In Silico PCR
Genome Browser

Rank: 46	Homology Score: 2	RVD Match Score: 2.58	Left Half-Site RVD Score: 5.483	Right Half-Site RVD Score: 7.802
	Genomic Region: Intron	Closest Gene: PAPPA
Match Type: R22L
	          Right Nuclease
	         TCAGCCTTTTGCAGTTTAT
	5'-CAGCT CCAACCTTTAGCTTTTAAT     TTTTTTAGCCTCATGAAAGAAA     CCAGATTGTTGACCTAAAT TCCCC-3'
	3'-GTCGA GGTTGGAAATCGAAAATTA     AAAAAATCGGAGTACTTTCTTT     GGTCTAACAACTGGATTTA AGGGG-5'
	                                                            GGTCGTACAACGGGTGTTT
	                                                                   Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 009-118942077
Forward Primer: 5'-GGATTGCTCTCTCAAGCATTCCTC-3' Tm: 59.038
Reverse Primer: 5'-TCCCAGCGATTACAGGTATCCC-3' Tm: 59.258
Expected PCR Product Length: 331
Expected CelI Cleavage Product Sizes: 128, 203
In Silico PCR
Genome Browser

Rank: 47	Homology Score: 2	RVD Match Score: 2.581	Left Half-Site RVD Score: 6.488	Right Half-Site RVD Score: 6.679
	Genomic Region: Intron	Closest Gene: HS3ST4
Match Type: L13R
	           Left Nuclease
	         TTTGTGGGCAACATGCTGG
	5'-TTGGG CTTGCATGCAACATACAGG         GCAGCTTGTTGTT          ATAAAAGGGAAGTGGTTGA CTTCT-3'
	3'-AACCC GAACGTACGTTGTATGTCC         CGTCGAACAACAA          TATTTTCCCTTCACCAACT GAAGA-5'
	                                                            TATTTGACGTTTTCCGACT
	                                                                  Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 016-025792276
Forward Primer: 5'-AGTAGAAGGCTGGCCCCACA-3' Tm: 59.508
Reverse Primer: 5'-CTCCAAAGAAACCCATCAGCAGTC-3' Tm: 59.038
Expected PCR Product Length: 357
Expected CelI Cleavage Product Sizes: 138, 219
In Silico PCR
Genome Browser

Rank: 48	Homology Score: 5	RVD Match Score: 2.583	Left Half-Site RVD Score: 6.481	Right Half-Site RVD Score: 6.952
	Genomic Region: Intergenic	Closest Gene: GRIN2A
Match Type: L18L
	           Left Nuclease
	         TTTGTGGGCAACATGCTGG
	5'-ATCCT TTTTTGTACAACATTCTAA       AAATCCTCATAGCCACTG       CCAGGAGGCTGCTCAGAAA GCTTC-3'
	3'-TAGGA AAAAACATGTTGTAAGATT       TTTAGGAGTATCGGTGAC       GGTCCTCCGACGAGTCTTT CGAAG-5'
	                                                            GGTCGTACAACGGGTGTTT
	                                                                   Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 016-009691841
Forward Primer: 5'-GATTGGAAGTCCCAGCACATGACA-3' Tm: 59.038
Reverse Primer: 5'-CCTTTGTTGCATAAGCCGTCACTG-3' Tm: 59.038
Expected PCR Product Length: 331
Expected CelI Cleavage Product Sizes: 204, 127
In Silico PCR
Genome Browser

Plate format Oligo Order Form
Well
Name
Sequence
5' mods
Internal
Notes
Primer Plate #1
A1
003-046414377F
GCACAGGGTGGAACAAGATGG
12212012SXJ93rlv_QUEUE_Ranking-RVDs	Primer pair generated using optimal parameters. 
A2
003-046414377R
GTGACCGTCCTGGCTTTTAAAGCA
12212012SXJ93rlv_QUEUE_Ranking-RVDs	Primer pair generated using optimal parameters. 
B1
003-046399009F
CATCCACAACATGCTGTCCACATC
12212012SXJ93rlv_QUEUE_Ranking-RVDs	Primer pair generated using optimal parameters. 
B2
003-046399009R
CAAAGACCCACTCATTTGCAGCAG
12212012SXJ93rlv_QUEUE_Ranking-RVDs	Primer pair generated using optimal parameters. 
C1
005-078100805F
TAGCAGGGACACAGCATCCG
12212012SXJ93rlv_QUEUE_Ranking-RVDs	Primer pair generated using optimal parameters. 
C2
005-078100805R
CTCGTTGGCTGACTTATGCTCTG
12212012SXJ93rlv_QUEUE_Ranking-RVDs	Primer pair generated using optimal parameters. 
D1
005-091821506F
AGGACTGGCTCCTTGTGGTG
12212012SXJ93rlv_QUEUE_Ranking-RVDs	Primer pair generated using optimal parameters. 
D2
005-091821506R
GACCTGCTTCTCTGGTCTTTTCTG
12212012SXJ93rlv_QUEUE_Ranking-RVDs	Primer pair generated using optimal parameters. 
E1
005-118490138F
GTCTAGATGGATCGAACTAGCCAC
12212012SXJ93rlv_QUEUE_Ranking-RVDs	Primer pair generated using optimal parameters. 
E2
005-118490138R
GCACTGACTGCTCTCTAACTACG
12212012SXJ93rlv_QUEUE_Ranking-RVDs	Primer pair generated using optimal parameters. 
F1
020-044711346F
GTGTCCAAGTGGTGGTCTGAAAAC
12212012SXJ93rlv_QUEUE_Ranking-RVDs	Primer pair generated using optimal parameters. 
F2
020-044711346R
CCGCTCTTGTTATTCCTAGTCCTC
12212012SXJ93rlv_QUEUE_Ranking-RVDs	Primer pair generated using optimal parameters. 
G1
003-157066450F
AAGGAAGAGGGGGCTGCAGA
12212012SXJ93rlv_QUEUE_Ranking-RVDs	Primer pair generated using optimal parameters. 
G2
003-157066450R
GCTGAGTCCTTGTCTGGTTTTGG
12212012SXJ93rlv_QUEUE_Ranking-RVDs	Primer pair generated using optimal parameters. 
H1
009-125759999F
GGCTCTGTCCCAAAGGAATCAC
12212012SXJ93rlv_QUEUE_Ranking-RVDs	Primer pair generated using optimal parameters. 
H2
009-125759999R
GTCAGATTGTTCTGCACAAGTGGC
12212012SXJ93rlv_QUEUE_Ranking-RVDs	Primer pair generated using optimal parameters. 
A3
002-175789857F
GACTCCCTCTTGGCCAACAG
12212012SXJ93rlv_QUEUE_Ranking-RVDs	Primer pair generated using optimal parameters. 
A4
002-175789857R
CAGTTGCAAATTCCCCCACAAGG
12212012SXJ93rlv_QUEUE_Ranking-RVDs	Primer pair generated using optimal parameters. 
B3
002-066451889F
CATATAGGATCCTCGATCACCCTG
12212012SXJ93rlv_QUEUE_Ranking-RVDs	Primer pair generated using optimal parameters. 
B4
002-066451889R
TGGACGTACGCAAACCCTGG
12212012SXJ93rlv_QUEUE_Ranking-RVDs	Primer pair generated using optimal parameters. 
C3
004-113138626F
GCCATCCAAATGTGGACAGTAGG
12212012SXJ93rlv_QUEUE_Ranking-RVDs	Primer pair generated using optimal parameters. 
C4
004-113138626R
GTAGGGGAAACCAATTACAGTGCC
12212012SXJ93rlv_QUEUE_Ranking-RVDs	Primer pair generated using optimal parameters. 
D3
002-039561318F
CCCTCTGCATGATCTGGTTGCATT
12212012SXJ93rlv_QUEUE_Ranking-RVDs	Primer pair generated using optimal parameters. 
D4
002-039561318R
GTAGTATCAGTGGGTGGGTGC
12212012SXJ93rlv_QUEUE_Ranking-RVDs	Primer pair generated using optimal parameters. 
E3
003-015921847F
GCTCTAGAACTCTAGACTGTGGG
12212012SXJ93rlv_QUEUE_Ranking-RVDs	Primer pair generated using optimal parameters. 
E4
003-015921847R
CTCACGGCCAATCCTGTTTTATCC
12212012SXJ93rlv_QUEUE_Ranking-RVDs	Primer pair generated using optimal parameters. 
F3
004-038011091F
GTTTCAGGAGCTCTTTCCTAAATCCTTGTG
12212012SXJ93rlv_QUEUE_Ranking-RVDs	Primer pair generated using optimal parameters. 
F4
004-038011091R
GACAGATTCAATAAGGGTGTGGGG
12212012SXJ93rlv_QUEUE_Ranking-RVDs	Primer pair generated using optimal parameters. 
G3
017-025719104F
CATCTGTCCTTCAGGACTTGAGAG
12212012SXJ93rlv_QUEUE_Ranking-RVDs	Primer pair generated using optimal parameters. 
G4
017-025719104R
GGGATAGCCACTGGAAAAGGG
12212012SXJ93rlv_QUEUE_Ranking-RVDs	Primer pair generated using optimal parameters. 
H3
00X-109896865F
CCATTGTACAGAGTGGTAACTCCAGTG
12212012SXJ93rlv_QUEUE_Ranking-RVDs	Primer pair generated using optimal parameters. 
H4
00X-109896865R
GTTAGGGGGGAGTCCCTTCT
12212012SXJ93rlv_QUEUE_Ranking-RVDs	Primer pair generated using optimal parameters. 
A5
021-039646075F
CCTGGGCAAAGTCATGTTAGGTG
12212012SXJ93rlv_QUEUE_Ranking-RVDs	Primer pair generated using optimal parameters. 
A6
021-039646075R
CAAGGCATTTGCTCTCATGAGGC
12212012SXJ93rlv_QUEUE_Ranking-RVDs	Primer pair generated using optimal parameters. 
B5
005-105472401F
GCCTCTCTTTTCTAGGTGAAGACG
12212012SXJ93rlv_QUEUE_Ranking-RVDs	Primer pair generated using optimal parameters. 
B6
005-105472401R
GAGGAAGAGGGGGACTGACA
12212012SXJ93rlv_QUEUE_Ranking-RVDs	Primer pair generated using optimal parameters. 
C5
001-246821429F
CAGCCAGATTCAAGATCCTCTCC
12212012SXJ93rlv_QUEUE_Ranking-RVDs	Primer pair generated using optimal parameters. 
C6
001-246821429R
GCTTCCCTGAATGTGGGTCTATAG
12212012SXJ93rlv_QUEUE_Ranking-RVDs	Primer pair generated using optimal parameters. 
D5
007-036433669F
ATCCTTTTGCTTCAGCCACCCC
12212012SXJ93rlv_QUEUE_Ranking-RVDs	Primer pair generated using optimal parameters. 
D6
007-036433669R
AGATCCCCTCTTGATGAAGGGGAC
12212012SXJ93rlv_QUEUE_Ranking-RVDs	Primer pair generated using optimal parameters. 
E5
013-089954123F
GAGTGTGTTGGGGAGATATTGGTC
12212012SXJ93rlv_QUEUE_Ranking-RVDs	Primer pair generated using optimal parameters. 
E6
013-089954123R
CTAGTGACTTCAGTGTGTATGCATGAAGC
12212012SXJ93rlv_QUEUE_Ranking-RVDs	Primer pair generated using optimal parameters. 
F5
009-005901106F
CTTAACCTATGGTGAGTGAGAGGC
12212012SXJ93rlv_QUEUE_Ranking-RVDs	Primer pair generated using optimal parameters. 
F6
009-005901106R
GGTGACAGAATGAGACCCCATC
12212012SXJ93rlv_QUEUE_Ranking-RVDs	Primer pair generated using optimal parameters. 
G5
002-119344377F
GTGGGTTAGAAGGTGGGAACAG
12212012SXJ93rlv_QUEUE_Ranking-RVDs	Primer pair generated using optimal parameters. 
G6
002-119344377R
GAACTAGATGCCCATGTGGGTC
12212012SXJ93rlv_QUEUE_Ranking-RVDs	Primer pair generated using optimal parameters. 
H5
018-001712330F
CAGGGACATTAACTGTTTGGAGGG
12212012SXJ93rlv_QUEUE_Ranking-RVDs	Primer pair generated using optimal parameters. 
H6
018-001712330R
CCTAGGCTTGTCAATAGACAGGAG
12212012SXJ93rlv_QUEUE_Ranking-RVDs	Primer pair generated using optimal parameters. 
A7
016-062697711F
ACTGAATGGACCCCTCGTGG
12212012SXJ93rlv_QUEUE_Ranking-RVDs	Primer pair generated using optimal parameters. 
A8
016-062697711R
GGCTTTGCAGGGCCATTTCAAAC
12212012SXJ93rlv_QUEUE_Ranking-RVDs	Primer pair generated using optimal parameters. 
B7
001-023678545F
GAGTGCTGAACACTCAGTCACAAC
12212012SXJ93rlv_QUEUE_Ranking-RVDs	Primer pair generated using optimal parameters. 
B8
001-023678545R
GATCTTCCCACCTCAGCCTC
12212012SXJ93rlv_QUEUE_Ranking-RVDs	Primer pair generated using optimal parameters. 
C7
015-056932411F
CAAGCCATATTGAAGCCTGAGGCA
12212012SXJ93rlv_QUEUE_Ranking-RVDs	Primer pair generated using optimal parameters. 
C8
015-056932411R
GCATTTGCTTCCATTAGCCAGGAG
12212012SXJ93rlv_QUEUE_Ranking-RVDs	Primer pair generated using optimal parameters. 
D7
001-234812284F
GCCTGCCTGCTGAAGACATAAG
12212012SXJ93rlv_QUEUE_Ranking-RVDs	Primer pair generated using optimal parameters. 
D8
001-234812284R
CGCCTGACTTTATTGAATGCAGCC
12212012SXJ93rlv_QUEUE_Ranking-RVDs	Primer pair generated using optimal parameters. 
E7
005-025770689F
GGAGGGAGGTATTGCCATGAAG
12212012SXJ93rlv_QUEUE_Ranking-RVDs	Primer pair generated using optimal parameters. 
E8
005-025770689R
CTTCTACCACATAGTTGGGCTGC
12212012SXJ93rlv_QUEUE_Ranking-RVDs	Primer pair generated using optimal parameters. 
F7
022-034808553F
CATGAGGAAGTGTCAGACAAACCC
12212012SXJ93rlv_QUEUE_Ranking-RVDs	Primer pair generated using optimal parameters. 
F8
022-034808553R
GCGGCAGAGGATCAGGAAAG
12212012SXJ93rlv_QUEUE_Ranking-RVDs	Primer pair generated using optimal parameters. 
G7
015-076238324F
CTCCTGCACTTTGGGGCCAT
12212012SXJ93rlv_QUEUE_Ranking-RVDs	Primer pair generated using optimal parameters. 
G8
015-076238324R
GACAGAGCGAGACCTCGTCTCA
12212012SXJ93rlv_QUEUE_Ranking-RVDs	Primer pair generated using optimal parameters. 
H7
003-159856803F
CCTTGGCGACAGAGCAAGAC
12212012SXJ93rlv_QUEUE_Ranking-RVDs	Primer pair generated using optimal parameters. 
H8
003-159856803R
CACGCACACACACACACGATAAG
12212012SXJ93rlv_QUEUE_Ranking-RVDs	Primer pair generated using optimal parameters. 
A9
017-070934484F
AAAGCCCAGTTCCACATCTTCCTC
12212012SXJ93rlv_QUEUE_Ranking-RVDs	Primer pair generated using optimal parameters. 
A10
017-070934484R
GGTAGCAGCAGCCTTCATTAGGA
12212012SXJ93rlv_QUEUE_Ranking-RVDs	Primer pair generated using optimal parameters. 
B9
003-110537278F
GTGGGTCTCTCTTCACTTTATTGATTGTTTCC
12212012SXJ93rlv_QUEUE_Ranking-RVDs	Warning! Primer pair was generated using suboptimal parameters. 
B10
003-110537278R
GGTAAGAAGAGCGAAACTCCGTC
12212012SXJ93rlv_QUEUE_Ranking-RVDs	Warning! Primer pair was generated using suboptimal parameters. 
C9
007-036783522F
GGTTGTTTCTCTTCACTCACTGGC
12212012SXJ93rlv_QUEUE_Ranking-RVDs	Primer pair generated using optimal parameters. 
C10
007-036783522R
GCCCAACAATGCTGAACACAGATG
12212012SXJ93rlv_QUEUE_Ranking-RVDs	Primer pair generated using optimal parameters. 
D9
014-079451010F
ACTCAGATTCCTCAATTCTGCCCC
12212012SXJ93rlv_QUEUE_Ranking-RVDs	Primer pair generated using optimal parameters. 
D10
014-079451010R
GGGCAAAGGCCATGTAGAATCAG
12212012SXJ93rlv_QUEUE_Ranking-RVDs	Primer pair generated using optimal parameters. 
E9
004-065322559F
GAGCTGATGGACCTGAAGGTAC
12212012SXJ93rlv_QUEUE_Ranking-RVDs	Primer pair generated using optimal parameters. 
E10
004-065322559R
GCCACTCCATATGGCCATAAGTAG
12212012SXJ93rlv_QUEUE_Ranking-RVDs	Primer pair generated using optimal parameters. 
F9
008-104699899F
CATACCCCAGTCTCTGGTGATC
12212012SXJ93rlv_QUEUE_Ranking-RVDs	Primer pair generated using optimal parameters. 
F10
008-104699899R
CTCAACGGTGAAAAGTTGGAAACTTTCCTC
12212012SXJ93rlv_QUEUE_Ranking-RVDs	Primer pair generated using optimal parameters. 
G9
008-051977566F
CAGAGATCGCACCATTGCATTCC
12212012SXJ93rlv_QUEUE_Ranking-RVDs	Primer pair generated using optimal parameters. 
G10
008-051977566R
GTCTAAGAATCTGTGGGCAAATGTATGGTC
12212012SXJ93rlv_QUEUE_Ranking-RVDs	Primer pair generated using optimal parameters. 
H9
00Y-019313071F
GTGGGAGCCGTGTCCATTGA
12212012SXJ93rlv_QUEUE_Ranking-RVDs	Primer pair generated using optimal parameters. 
H10
00Y-019313071R
GCTACCAAAGACTCTTGCAAAGGG
12212012SXJ93rlv_QUEUE_Ranking-RVDs	Primer pair generated using optimal parameters. 
A11
013-061919703F
CCTGGTCTGTTCTAGTGGCTC
12212012SXJ93rlv_QUEUE_Ranking-RVDs	Primer pair generated using optimal parameters. 
A12
013-061919703R
GAGAAGTTCAACAGTTGAGGCAATCTGTG
12212012SXJ93rlv_QUEUE_Ranking-RVDs	Primer pair generated using optimal parameters. 
B11
00X-032184490F
TCTGAGCCCAGGGAGGTCAA
12212012SXJ93rlv_QUEUE_Ranking-RVDs	Primer pair generated using optimal parameters. 
B12
00X-032184490R
CACAGAAGCAGCAGGAGCAAG
12212012SXJ93rlv_QUEUE_Ranking-RVDs	Primer pair generated using optimal parameters. 
C11
017-057059452F
GTCTAAAGTGAGCCCAGAGGCC
12212012SXJ93rlv_QUEUE_Ranking-RVDs	Primer pair generated using optimal parameters. 
C12
017-057059452R
CTGCTGTTGACTAGGGGAGG
12212012SXJ93rlv_QUEUE_Ranking-RVDs	Primer pair generated using optimal parameters. 
D11
001-007840539F
AGGCTGTAGACAGTGTGGCTC
12212012SXJ93rlv_QUEUE_Ranking-RVDs	Primer pair generated using optimal parameters. 
D12
001-007840539R
CATTCACCCCTGACCAATGCC
12212012SXJ93rlv_QUEUE_Ranking-RVDs	Primer pair generated using optimal parameters. 
E11
002-210791640F
CATCTGTGCAGCAGTACTTCCC
12212012SXJ93rlv_QUEUE_Ranking-RVDs	Primer pair generated using optimal parameters. 
E12
002-210791640R
TTCTCTGGTCACGAACAATGCTCC
12212012SXJ93rlv_QUEUE_Ranking-RVDs	Primer pair generated using optimal parameters. 
F11
009-118942077F
GGATTGCTCTCTCAAGCATTCCTC
12212012SXJ93rlv_QUEUE_Ranking-RVDs	Primer pair generated using optimal parameters. 
F12
009-118942077R
TCCCAGCGATTACAGGTATCCC
12212012SXJ93rlv_QUEUE_Ranking-RVDs	Primer pair generated using optimal parameters. 
G11
016-025792276F
AGTAGAAGGCTGGCCCCACA
12212012SXJ93rlv_QUEUE_Ranking-RVDs	Primer pair generated using optimal parameters. 
G12
016-025792276R
CTCCAAAGAAACCCATCAGCAGTC
12212012SXJ93rlv_QUEUE_Ranking-RVDs	Primer pair generated using optimal parameters. 
H11
016-009691841F
GATTGGAAGTCCCAGCACATGACA
12212012SXJ93rlv_QUEUE_Ranking-RVDs	Primer pair generated using optimal parameters. 
H12
016-009691841R
CCTTTGTTGCATAAGCCGTCACTG
12212012SXJ93rlv_QUEUE_Ranking-RVDs	Primer pair generated using optimal parameters.