PROGNOS Summary for 12212012SXJ93rlv_QUEUE_Ranking-Homology_5T

The hg19 genome was searched for the TALEN pair targeting:
5'- TTTGTGGGCAACATGCTGG NN..NN ATAAACTGCAAAAGGCTGA -3'

Up to 6 mismatches were allowed in each nuclease half-site.

Allowed spacing distances were: 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30

Homodimers and heterodimers of the nucleases were included in the output

2331 total sites were located
Mismatch Types
Genomic Regions
  0-0: 1
  0-1: 1
  3-5: 4
  4-4: 1
  3-6: 13
  4-5: 11
  4-6: 68
  5-5: 45
  5-6: 565
  6-6: 1622
  Exons: 51
  Promoters: 10
  Introns: 908
  Intergenic: 1362


The top 48 sites are ranked below according to a weighted sequence homology. All sites listed contain flanking 5' thymidines.
For the full output, click here.

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Primer pairs were designed using the 422 bp of sequence on either side of the cleavage site to melt close to 60 °C

For easy visualization of cleavage on gels:
The smallest fragment will be longer than 125 bp, which is also the minimum separation distance between uncleaved and cleaved products.
The two cleavage products will be separated by a minimum size of 75 bp
Rank: 1	Homology Score: 98
	Genomic Region: Exon	Closest Gene: CCR5
Match Type: L14R
	           Left Nuclease
	         TTTGTGGGCAACATGCTGG
	5'-TTGGT TTTGTGGGCAACATGCTGG         TCATCCTCATCCTG         ATAAACTGCAAAAGGCTGA AGAGC-3'
	3'-AACCA AAACACCCGTTGTACGACC         AGTAGGAGTAGGAC         TATTTGACGTTTTCCGACT TCTCG-5'
	                                                            TATTTGACGTTTTCCGACT
	                                                                  Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 003-046414377
Forward Primer: 5'-GCACAGGGTGGAACAAGATGG-3' Tm: 59.378
Reverse Primer: 5'-GTGACCGTCCTGGCTTTTAAAGCA-3' Tm: 59.038
Expected PCR Product Length: 446
Expected CelI Cleavage Product Sizes: 177, 269
In Silico PCR
Genome Browser

Rank: 2	Homology Score: 85
	Genomic Region: Exon	Closest Gene: CCR2
Match Type: L14R
	           Left Nuclease
	         TTTGTGGGCAACATGCTGG
	5'-TTGGT TTTGTGGGCAACATGCTGG         TCGTCCTCATCTTA         ATAAACTGCAAAAAGCTGA AGTGC-3'
	3'-AACCA AAACACCCGTTGTACGACC         AGCAGGAGTAGAAT         TATTTGACGTTTTTCGACT TCACG-5'
	                                                            TATTTGACGTTTTCCGACT
	                                                                  Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 003-046399009
Forward Primer: 5'-CATCCACAACATGCTGTCCACATC-3' Tm: 59.038
Reverse Primer: 5'-CAAAGACCCACTCATTTGCAGCAG-3' Tm: 59.038
Expected PCR Product Length: 336
Expected CelI Cleavage Product Sizes: 206, 130
In Silico PCR
Genome Browser

Rank: 3	Homology Score: 20
	Genomic Region: Intron	Closest Gene: LOC283038
Match Type: L17L
	           Left Nuclease
	         TTTGTGGGCAACATGCTGG
	5'-TCCTT TTTGAGGGGAAGATGCTGG       GTTTATTTAAAACACAT        GCAACATGTTGGACACACA CACAC-3'
	3'-AGGAA AAACTCCCCTTCTACGACC       CAAATAAATTTTGTGTA        CGTTGTACAACCTGTGTGT GTGTG-5'
	                                                            GGTCGTACAACGGGTGTTT
	                                                                   Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 010-127381042
Forward Primer: 5'-CATCTCACGCAAAAAGACACCACC-3' Tm: 59.038
Reverse Primer: 5'-GGACTCACAAGGACAGGCTC-3' Tm: 59.508
Expected PCR Product Length: 479
Expected CelI Cleavage Product Sizes: 282, 197
In Silico PCR
Genome Browser

Rank: 4	Homology Score: 20
	Genomic Region: Intron	Closest Gene: SFMBT2
Match Type: L11L
	           Left Nuclease
	         TTTGTGGGCAACATGCTGG
	5'-AGCCA TTTGGGGATAACATGATGA          GAAGGAGATAT           CCATCATGTATCCCACAAA CATTA-3'
	3'-TCGGT AAACCCCTATTGTACTACT          CTTCCTCTATA           GGTAGTACATAGGGTGTTT GTAAT-5'
	                                                            GGTCGTACAACGGGTGTTT
	                                                                   Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 010-007305349
Forward Primer: 5'-CGTGCCACTAATGCCCAGGA-3' Tm: 59.508
Reverse Primer: 5'-TTTGGCCTTCAGGTGGGACACT-3' Tm: 59.258
Expected PCR Product Length: 329
Expected CelI Cleavage Product Sizes: 203, 126
In Silico PCR
Genome Browser

Rank: 5	Homology Score: 20
	Genomic Region: Intergenic	Closest Gene: LOC285401
Match Type: R12R
	          Right Nuclease
	         TCAGCCTTTTGCAGTTTAT
	5'-ACTCC TCAGCCTGTTTCAGTTTTT          AAAATATGTATT          ATAAAATTCATAATGCTAA GTCAC-3'
	3'-TGAGG AGTCGGACAAAGTCAAAAA          TTTTATACATAA          TATTTTAAGTATTACGATT CAGTG-5'
	                                                            TATTTGACGTTTTCCGACT
	                                                                  Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 003-063153302
Forward Primer: 5'-TCTGGGCTGGGCTGTGCTTT-3' Tm: 59.508
Reverse Primer: 5'-CCTAGTTGCTCCTTAGTACTTCCC-3' Tm: 59.038
Expected PCR Product Length: 340
Expected CelI Cleavage Product Sizes: 126, 214
In Silico PCR
Genome Browser

Rank: 6	Homology Score: 20
	Genomic Region: Intergenic	Closest Gene: TRMT44
Match Type: L22L
	           Left Nuclease
	         TTTGTGGGCAACATGCTGG
	5'-TCCAG TTTGAGGGGGAAATGCTTG     GGTATGAGCTCCCTCCCAGGAC     CCAGCGTGGTGCCCACACA TCATG-3'
	3'-AGGTC AAACTCCCCCTTTACGAAC     CCATACTCGAGGGAGGGTCCTG     GGTCGCACCACGGGTGTGT AGTAC-5'
	                                                            GGTCGTACAACGGGTGTTT
	                                                                   Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 004-008527974
Forward Primer: 5'-GCTCAAGTGTGAGGGTGCTG-3' Tm: 59.508
Reverse Primer: 5'-TAAGGAGGCTGGAGGGAGCA-3' Tm: 59.508
Expected PCR Product Length: 341
Expected CelI Cleavage Product Sizes: 208, 133
In Silico PCR
Genome Browser

Rank: 7	Homology Score: 18
	Genomic Region: Intron	Closest Gene: DPP10
Match Type: R18R
	          Right Nuclease
	         TCAGCCTTTTGCAGTTTAT
	5'-CACCT TCTGCATTTTTCAGTTTGT       TTTCTTATCAGCCTGCCC       ATAAACTGGAAAAGCATTA GGCAC-3'
	3'-GTGGA AGACGTAAAAAGTCAAACA       AAAGAATAGTCGGACGGG       TATTTGACCTTTTCGTAAT CCGTG-5'
	                                                            TATTTGACGTTTTCCGACT
	                                                                  Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 002-116474320
Forward Primer: 5'-CATTTGTGGCCAAAGGTGCCTTGA-3' Tm: 59.038
Reverse Primer: 5'-CCCTCCCTCTCATTCATCCAAAG-3' Tm: 59.148
Expected PCR Product Length: 361
Expected CelI Cleavage Product Sizes: 129, 232
In Silico PCR
Genome Browser

Rank: 8	Homology Score: 17
	Genomic Region: Exon	Closest Gene: BAZ2B
Match Type: R29R
	          Right Nuclease
	         TCAGCCTTTTGCAGTTTAT
	5'-CACTT TCTGCCTTTTTCAGTTTTT CTCTTTCTTTTGCAATTTCTTCTAGTCCT  ACAAAATGAAAAAGCATTA TGAAC-3'
	3'-GTGAA AGACGGAAAAAGTCAAAAA GAGAAAGAAAACGTTAAAGAAGATCAGGA  TGTTTTACTTTTTCGTAAT ACTTG-5'
	                                                            TATTTGACGTTTTCCGACT
	                                                                  Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 002-160206641
Forward Primer: 5'-CAGGAGGCATCTTAGCTACTTCCA-3' Tm: 59.038
Reverse Primer: 5'-AATTCACGCCATTGCACTCCAGC-3' Tm: 59.148
Expected PCR Product Length: 510
Expected CelI Cleavage Product Sizes: 217, 293
In Silico PCR
Genome Browser

Rank: 9	Homology Score: 17
	Genomic Region: Intron	Closest Gene: VEPH1
Match Type: R29L
	          Right Nuclease
	         TCAGCCTTTTGCAGTTTAT
	5'-TCCAG TCAAATTTTAGCTATTTAT CCATTGCTGTGGTCCTCTGACCCCTTGGG  CCAGCAGGTTGGCCACATA GGCAC-3'
	3'-AGGTC AGTTTAAAATCGATAAATA GGTAACGACACCAGGAGACTGGGGAACCC  GGTCGTCCAACCGGTGTAT CCGTG-5'
	                                                            GGTCGTACAACGGGTGTTT
	                                                                   Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 003-157066450
Forward Primer: 5'-AAGGAAGAGGGGGCTGCAGA-3' Tm: 59.508
Reverse Primer: 5'-GCTGAGTCCTTGTCTGGTTTTGG-3' Tm: 59.148
Expected PCR Product Length: 388
Expected CelI Cleavage Product Sizes: 153, 235
In Silico PCR
Genome Browser

Rank: 10	Homology Score: 17
	Genomic Region: Intron	Closest Gene: KCNIP4
Match Type: L30R
	           Left Nuclease
	         TTTGTGGGCAACATGCTGG
	5'-TATTT TTAATGGGCAACATGTTGG CCATTCAAGTTGCTTTTTGCAAACAAATCC TCCAACAGCCAAATGCTGA TGGTT-3'
	3'-ATAAA AATTACCCGTTGTACAACC GGTAAGTTCAACGAAAAACGTTTGTTTAGG AGGTTGTCGGTTTACGACT ACCAA-5'
	                                                            TATTTGACGTTTTCCGACT
	                                                                  Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 004-020877272
Forward Primer: 5'-GCCTAGGTCAGAGCCTTTAGC-3' Tm: 59.378
Reverse Primer: 5'-GCACTATCTCAGTGCCCTCATTC-3' Tm: 59.148
Expected PCR Product Length: 483
Expected CelI Cleavage Product Sizes: 144, 339
In Silico PCR
Genome Browser

Rank: 11	Homology Score: 17
	Genomic Region: Intron	Closest Gene: PI4KA
Match Type: R26R
	          Right Nuclease
	         TCAGCCTTTTGCAGTTTAT
	5'-TAATA TTAGCCTTTTGCAATTTAA   AAAAATTAAGGTATAATTTGCATATA   ATAAAATCCACAGGTCTTA TGTGC-3'
	3'-ATTAT AATCGGAAAACGTTAAATT   TTTTTAATTCCATATTAAACGTATAT   TATTTTAGGTGTCCAGAAT ACACG-5'
	                                                            TATTTGACGTTTTCCGACT
	                                                                  Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 022-021114662
Forward Primer: 5'-GTGCCCTTACACTAAAACTGGCC-3' Tm: 59.148
Reverse Primer: 5'-CTGGGAAGACAGAGAAATGGTGG-3' Tm: 59.148
Expected PCR Product Length: 398
Expected CelI Cleavage Product Sizes: 150, 248
In Silico PCR
Genome Browser

Rank: 12	Homology Score: 17
	Genomic Region: Intron	Closest Gene: ACER3
Match Type: R27R
	          Right Nuclease
	         TCAGCCTTTTGCAGTTTAT
	5'-TTTTG TCAGTCTTATGATCTCTAT  TTTAACCTTAATCATGGGCAGTTGTGC   CTAAACTCCAAAAGGGTGA GGATT-3'
	3'-AAAAC AGTCAGAATACTAGAGATA  AAATTGGAATTAGTACCCGTCAACACG   GATTTGAGGTTTTCCCACT CCTAA-5'
	                                                            TATTTGACGTTTTCCGACT
	                                                                  Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 011-076680948
Forward Primer: 5'-CCTGCATATTGTGCACATGTACCC-3' Tm: 59.038
Reverse Primer: 5'-GGATGAACCCCTCTTGGCCA-3' Tm: 59.508
Expected PCR Product Length: 355
Expected CelI Cleavage Product Sizes: 224, 131
In Silico PCR
Genome Browser

Rank: 13	Homology Score: 17
	Genomic Region: Intron	Closest Gene: EPHA3
Match Type: R20R
	          Right Nuclease
	         TCAGCCTTTTGCAGTTTAT
	5'-TCCTC TCTCCCTTTTCCTGTTTTC      TGCTCACTGCATACTACAAC      ATAAACTCCAAAAGGGAGA GGTTC-3'
	3'-AGGAG AGAGGGAAAAGGACAAAAG      ACGAGTGACGTATGATGTTG      TATTTGAGGTTTTCCCTCT CCAAG-5'
	                                                            TATTTGACGTTTTCCGACT
	                                                                  Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 003-089353684
Forward Primer: 5'-GTTCACTAGGCCAGGAACAGTC-3' Tm: 59.258
Reverse Primer: 5'-GCATGGCTAAAGAAGGCCAACATC-3' Tm: 59.038
Expected PCR Product Length: 460
Expected CelI Cleavage Product Sizes: 268, 192
In Silico PCR
Genome Browser

Rank: 14	Homology Score: 17
	Genomic Region: Intergenic	Closest Gene: MRPS9
Match Type: R27R
	          Right Nuclease
	         TCAGCCTTTTGCAGTTTAT
	5'-TACCT TCTCCCTTTTGCAGTTTAG  ACAAAACTGCCCGGCATTAATGTTATA   ATAGAGACCATAAGACTGA CAGAA-3'
	3'-ATGGA AGAGGGAAAACGTCAAATC  TGTTTTGACGGGCCGTAATTACAATAT   TATCTCTGGTATTCTGACT GTCTT-5'
	                                                            TATTTGACGTTTTCCGACT
	                                                                  Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 002-105729897
Forward Primer: 5'-TGAGTGGACTCCCTCTTGGC-3' Tm: 59.508
Reverse Primer: 5'-GGTGGATGAGCTGCAACTAGTTC-3' Tm: 59.148
Expected PCR Product Length: 351
Expected CelI Cleavage Product Sizes: 132, 219
In Silico PCR
Genome Browser

Rank: 15	Homology Score: 17
	Genomic Region: Intergenic	Closest Gene: ZNF433
Match Type: R26L
	          Right Nuclease
	         TCAGCCTTTTGCAGTTTAT
	5'-CCCAT TCAGCCTTCAGCAGTTCAT   CAATTACAATTTAAGTTTTTCTACCA   CAATACTGTTTCTCACAAA GGTTT-3'
	3'-GGGTA AGTCGGAAGTCGTCAAGTA   GTTAATGTTAAATTCAAAAAGATGGT   GTTATGACAAAGAGTGTTT CCAAA-5'
	                                                            GGTCGTACAACGGGTGTTT
	                                                                   Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 019-012111242
Forward Primer: 5'-GTAGTGTCGTCTCATGGGCAAAAG-3' Tm: 59.038
Reverse Primer: 5'-CAGTCCTGAGAGTGTGTTTCCTTG-3' Tm: 59.038
Expected PCR Product Length: 365
Expected CelI Cleavage Product Sizes: 135, 230
In Silico PCR
Genome Browser

Rank: 16	Homology Score: 17
	Genomic Region: Intergenic	Closest Gene: KCNMB2-IT1
Match Type: R27R
	          Right Nuclease
	         TCAGCCTTTTGCAGTTTAT
	5'-AAATT TCAGTATTTTGCATTTTAT  GACTCAAATGTTATGCTATTTGGGATT   CTAAACTTCAAAATCCCCA CAAAT-3'
	3'-TTTAA AGTCATAAAACGTAAAATA  CTGAGTTTACAATACGATAAACCCTAA   GATTTGAAGTTTTAGGGGT GTTTA-5'
	                                                            TATTTGACGTTTTCCGACT
	                                                                  Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 003-177863333
Forward Primer: 5'-CAGTCTGGAGACCTCCAGTAG-3' Tm: 59.378
Reverse Primer: 5'-GTCCTTCCAAGGCATCACCATTTC-3' Tm: 59.038
Expected PCR Product Length: 355
Expected CelI Cleavage Product Sizes: 137, 218
In Silico PCR
Genome Browser

Rank: 17	Homology Score: 17
	Genomic Region: Intergenic	Closest Gene: FAM89A
Match Type: R29R
	          Right Nuclease
	         TCAGCCTTTTGCAGTTTAT
	5'-TTCTG TCAGTCTTATGATCTCTAT TTAACATTAATGCTGGTTAGTCATTGTGT  CTAAACTGCAAAAGGGAGA GGTTA-3'
	3'-AAGAC AGTCAGAATACTAGAGATA AATTGTAATTACGACCAATCAGTAACACA  GATTTGACGTTTTCCCTCT CCAAT-5'
	                                                            TATTTGACGTTTTCCGACT
	                                                                  Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 001-231235899
Forward Primer: 5'-CTTGAGGCCAGTGCTTGTTTAGG-3' Tm: 59.148
Reverse Primer: 5'-TCCCCCTCTTGGCCAAAAGC-3' Tm: 59.508
Expected PCR Product Length: 326
Expected CelI Cleavage Product Sizes: 200, 126
In Silico PCR
Genome Browser

Rank: 18	Homology Score: 17
	Genomic Region: Intergenic	Closest Gene: GXYLT1
Match Type: R24R
	          Right Nuclease
	         TCAGCCTTTTGCAGTTTAT
	5'-TACCC TCTCCCTTTTGCAGTTTAG    ACACAATTCACCAGCATTAGTAAA    ATAGAGATCATAAGACTGA CAGAA-3'
	3'-ATGGG AGAGGGAAAACGTCAAATC    TGTGTTAAGTGGTCGTAATCATTT    TATCTCTAGTATTCTGACT GTCTT-5'
	                                                            TATTTGACGTTTTCCGACT
	                                                                  Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 012-042468830
Forward Primer: 5'-GAATGGACACCTACCTTGGCC-3' Tm: 59.378
Reverse Primer: 5'-GTCTGGCAATGGATCAGCTGCA-3' Tm: 59.258
Expected PCR Product Length: 362
Expected CelI Cleavage Product Sizes: 130, 232
In Silico PCR
Genome Browser

Rank: 19	Homology Score: 17
	Genomic Region: Intergenic	Closest Gene: TMPRSS11B
Match Type: R30R
	          Right Nuclease
	         TCAGCCTTTTGCAGTTTAT
	5'-TTATG TCAGTCTTAAGATGTCTAT TTTAACATTAATGCTAGTCAGCTGTTATGC CTAAACTGCAAAAGGGAGA GAGTA-3'
	3'-AATAC AGTCAGAATTCTACAGATA AAATTGTAATTACGATCAGTCGACAATACG GATTTGACGTTTTCCCTCT CTCAT-5'
	                                                            TATTTGACGTTTTCCGACT
	                                                                  Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 004-069131937
Forward Primer: 5'-ATGGCCTGTGGGTGAGGCTT-3' Tm: 59.508
Reverse Primer: 5'-GGAGAATCACTTGAACCCGGG-3' Tm: 59.378
Expected PCR Product Length: 346
Expected CelI Cleavage Product Sizes: 127, 219
In Silico PCR
Genome Browser

Rank: 20	Homology Score: 17
	Genomic Region: Intergenic	Closest Gene: FAM75C2
Match Type: L20R
	           Left Nuclease
	         TTTGTGGGCAACATGCTGG
	5'-TATGA TTTTTGGGCAACATAGTGG      GACTCTGTCTACAAAACTAA      ATAAATAGAAAGAGGCCCA GTGCG-3'
	3'-ATACT AAAAACCCGTTGTATCACC      CTGAGACAGATGTTTTGATT      TATTTATCTTTCTCCGGGT CACGC-5'
	                                                            TATTTGACGTTTTCCGACT
	                                                                  Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 009-090818650
Forward Primer: 5'-CTGCAAGGACTCAAAAAATTTGACCCCTAC-3' Tm: 60.058
Reverse Primer: 5'-TCACTATATGGGCCAGGCTGATC-3' Tm: 59.148
Expected PCR Product Length: 350
Expected CelI Cleavage Product Sizes: 225, 125
In Silico PCR
Genome Browser

Rank: 21	Homology Score: 13
	Genomic Region: Intron	Closest Gene: MAP3K5
Match Type: R27R
	          Right Nuclease
	         TCAGCCTTTTGCAGTTTAT
	5'-ATACC TCCTCCCTTTGGAGTTTAG  GCACAACTGACCAGCATTACCATTAAA   ATAAAGATCATAAGGCTGA CAAAA-3'
	3'-TATGG AGGAGGGAAACCTCAAATC  CGTGTTGACTGGTCGTAATGGTAATTT   TATTTCTAGTATTCCGACT GTTTT-5'
	                                                            TATTTGACGTTTTCCGACT
	                                                                  Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 006-136890071
Forward Primer: 5'-GAAGAACCACCTACCCCCAG-3' Tm: 59.508
Reverse Primer: 5'-CTACAGAATGCAGATTTCCCCCAC-3' Tm: 59.038
Expected PCR Product Length: 348
Expected CelI Cleavage Product Sizes: 135, 213
In Silico PCR
Genome Browser

Rank: 22	Homology Score: 13
	Genomic Region: Intron	Closest Gene: KCNA6
Match Type: R24L
	          Right Nuclease
	         TCAGCCTTTTGCAGTTTAT
	5'-TTATC TGAGTCTTCTGAATTTTAT    AGCGGAAAGAAGGGATGCAGAAGG    CCAGCATCTTACCCAGGAA CGCTG-3'
	3'-AATAG ACTCAGAAGACTTAAAATA    TCGCCTTTCTTCCCTACGTCTTCC    GGTCGTAGAATGGGTCCTT GCGAC-5'
	                                                            GGTCGTACAACGGGTGTTT
	                                                                   Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 012-004929936
Forward Primer: 5'-GGGAGGACTAATGACTGAGGG-3' Tm: 59.378
Reverse Primer: 5'-CTCACCCTGGTCCTGTGATTTG-3' Tm: 59.258
Expected PCR Product Length: 397
Expected CelI Cleavage Product Sizes: 236, 161
In Silico PCR
Genome Browser

Rank: 23	Homology Score: 13
	Genomic Region: Intron	Closest Gene: RNU6-71
Match Type: R25L
	          Right Nuclease
	         TCAGCCTTTTGCAGTTTAT
	5'-CGCGG TAAGCAGTTTGCTGTTTAG   GCACTTCTTTTCGACCAATCATGTC    CCAGTTAGTTGCCCCCAAA ATCAA-3'
	3'-GCGCC ATTCGTCAAACGACAAATC   CGTGAAGAAAAGCTGGTTAGTACAG    GGTCAATCAACGGGGGTTT TAGTT-5'
	                                                            GGTCGTACAACGGGTGTTT
	                                                                   Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 006-056297715
Forward Primer: 5'-TCTACTCTCTTGGTGTCGCCATG-3' Tm: 59.148
Reverse Primer: 5'-AGTGGTGACTAGGACCGCGT-3' Tm: 59.508
Expected PCR Product Length: 364
Expected CelI Cleavage Product Sizes: 219, 145
In Silico PCR
Genome Browser

Rank: 24	Homology Score: 13
	Genomic Region: Intron	Closest Gene: ZFHX4
Match Type: R21R
	          Right Nuclease
	         TCAGCCTTTTGCAGTTTAT
	5'-TAATG TAAGATTTTTGCAGTATTT     TAAGATCTTTTGATAGACTAA      TTATATTGCAAAAGGTTGA TTGCA-3'
	3'-ATTAC ATTCTAAAAACGTCATAAA     ATTCTAGAAAACTATCTGATT      AATATAACGTTTTCCAACT AACGT-5'
	                                                            TATTTGACGTTTTCCGACT
	                                                                  Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 008-077661832
Forward Primer: 5'-GGGCCAGACATTTCTTTAGCCTAG-3' Tm: 59.038
Reverse Primer: 5'-GGAGACATCCTCAGGGAAAGC-3' Tm: 59.378
Expected PCR Product Length: 612
Expected CelI Cleavage Product Sizes: 265, 347
In Silico PCR
Genome Browser

Rank: 25	Homology Score: 13
	Genomic Region: Intergenic	Closest Gene: YTHDC2
Match Type: R27R
	          Right Nuclease
	         TCAGCCTTTTGCAGTTTAT
	5'-TTATG TCAGCCTTTTGAGCGCTAT  TTTAACCTTAATGCTGGTCAATTGTGC   ATAAACTCCAAAAGGGAAA GGGTA-3'
	3'-AATAC AGTCGGAAAACTCGCGATA  AAATTGGAATTACGACCAGTTAACACG   TATTTGAGGTTTTCCCTTT CCCAT-5'
	                                                            TATTTGACGTTTTCCGACT
	                                                                  Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 005-112991631
Forward Primer: 5'-TACTCCAGCCTGTGTGACAAGAGC-3' Tm: 60.908
Reverse Primer: 5'-CTACATGGATCTCCTCTTGGCC-3' Tm: 59.258
Expected PCR Product Length: 338
Expected CelI Cleavage Product Sizes: 206, 132
In Silico PCR
Genome Browser

Rank: 26	Homology Score: 13
	Genomic Region: Intergenic	Closest Gene: HS3ST5
Match Type: R18L
	          Right Nuclease
	         TCAGCCTTTTGCAGTTTAT
	5'-CCCCA TCTCCCTCTTGCAGGTTAT       ACCCTAAGCCTAGAGCAA       CCAGCATCGTGATTACAAA TAAGG-3'
	3'-GGGGT AGAGGGAGAACGTCCAATA       TGGGATTCGGATCTCGTT       GGTCGTAGCACTAATGTTT ATTCC-5'
	                                                            GGTCGTACAACGGGTGTTT
	                                                                   Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 006-114639685
Forward Primer: 5'-CCCACTAAGGCTCTTCACAGCA-3' Tm: 59.258
Reverse Primer: 5'-TAGGCATTGAGCACTTGCTAGGTC-3' Tm: 59.038
Expected PCR Product Length: 343
Expected CelI Cleavage Product Sizes: 134, 209
In Silico PCR
Genome Browser

Rank: 27	Homology Score: 13
	Genomic Region: Intergenic	Closest Gene: MAT2B
Match Type: L18R
	           Left Nuclease
	         TTTGTGGGCAACATGCTGG
	5'-GGGAG TTTTTGTGGAACATGATGG       GATGACATTAAAATAGTA       TGAAAATGCAAAATGCTAA TAATA-3'
	3'-CCCTC AAAAACACCTTGTACTACC       CTACTGTAATTTTATCAT       ACTTTTACGTTTTACGATT ATTAT-5'
	                                                            TATTTGACGTTTTCCGACT
	                                                                  Right Nuclease
Warning! Primer pair was generated using suboptimal parameters. 
Primer Index: 005-164243933
Forward Primer: 5'-CAGTAGACAATACGTAATACCTCCTGCAAG-3' Tm: 60.058
Reverse Primer: 5'-CTGCCCTACTCCATTTTAAAGATATTCTCCC-3' Tm: 59.958
Expected PCR Product Length: 526
Expected CelI Cleavage Product Sizes: 221, 305
In Silico PCR
Genome Browser

Rank: 28	Homology Score: 13
	Genomic Region: Intergenic	Closest Gene: KDM5D
Match Type: R25L
	          Right Nuclease
	         TCAGCCTTTTGCAGTTTAT
	5'-TATGG TAAGCACTTTGTTGTTTAT   GGGCTTCTTTTCCACCTATCAATTC    CCAGTTGGTTGCCCCCAAA ATGAA-3'
	3'-ATACC ATTCGTGAAACAACAAATA   CCCGAAGAAAAGGTGGATAGTTAAG    GGTCAACCAACGGGGGTTT TACTT-5'
	                                                            GGTCGTACAACGGGTGTTT
	                                                                   Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 00Y-022149362
Forward Primer: 5'-CATGACTGACACCCTCCACG-3' Tm: 59.508
Reverse Primer: 5'-AGCAAAGCAGGTGGCACAGC-3' Tm: 59.508
Expected PCR Product Length: 330
Expected CelI Cleavage Product Sizes: 203, 127
In Silico PCR
Genome Browser

Rank: 29	Homology Score: 13
	Genomic Region: Intergenic	Closest Gene: OR7E156P
Match Type: R23R
	          Right Nuclease
	         TCAGCCTTTTGCAGTTTAT
	5'-CCTAC TGAGCCTTTTGCAGTACAA    ATCCCTGTGCTGTAAACAGTTGT     ATAAAATATAAAATTCTGA ACATT-3'
	3'-GGATG ACTCGGAAAACGTCATGTT    TAGGGACACGACATTTGTCAACA     TATTTTATATTTTAAGACT TGTAA-5'
	                                                            TATTTGACGTTTTCCGACT
	                                                                  Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 013-064120919
Forward Primer: 5'-CTCCTAGTCTACAAACCTCTGCAG-3' Tm: 59.038
Reverse Primer: 5'-CACCCTTAGCAAATAGGCAGAGAG-3' Tm: 59.038
Expected PCR Product Length: 461
Expected CelI Cleavage Product Sizes: 320, 141
In Silico PCR
Genome Browser

Rank: 30	Homology Score: 13
	Genomic Region: Intergenic	Closest Gene: MIR4300
Match Type: R28R
	          Right Nuclease
	         TCAGCCTTTTGCAGTTTAT
	5'-TTTGG TCTTCCTTTTCCACTTTAT  CTTTTATACTGCTATTGCTATGGTCATT  CTAAACTGGAAATAGCAGA AGTAG-3'
	3'-AAACC AGAAGGAAAAGGTGAAATA  GAAAATATGACGATAACGATACCAGTAA  GATTTGACCTTTATCGTCT TCATC-5'
	                                                            TATTTGACGTTTTCCGACT
	                                                                  Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 011-080866146
Forward Primer: 5'-CCCAGAAACCACCAACTAGCATG-3' Tm: 59.148
Reverse Primer: 5'-CTGGAGATCTGAGACCATGCG-3' Tm: 59.378
Expected PCR Product Length: 568
Expected CelI Cleavage Product Sizes: 396, 172
In Silico PCR
Genome Browser

Rank: 31	Homology Score: 13
	Genomic Region: Intergenic	Closest Gene: LOC728084
Match Type: L17R
	           Left Nuclease
	         TTTGTGGGCAACATGCTGG
	5'-CTTTA TCTGAGGGCAACATCCTAC       CACAATTCCCATAATAA        ATAGACTGCACAAGAGTGA GAAGC-3'
	3'-GAAAT AGACTCCCGTTGTAGGATG       GTGTTAAGGGTATTATT        TATCTGACGTGTTCTCACT CTTCG-5'
	                                                            TATTTGACGTTTTCCGACT
	                                                                  Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 012-089421427
Forward Primer: 5'-GCATTCACTCGCCTGTTCCTC-3' Tm: 59.378
Reverse Primer: 5'-GCTGACTTAAGAGGGCTCTTTGC-3' Tm: 59.148
Expected PCR Product Length: 332
Expected CelI Cleavage Product Sizes: 125, 207
In Silico PCR
Genome Browser

Rank: 32	Homology Score: 10
	Genomic Region: Exon	Closest Gene: DAPP1
Match Type: R20R
	          Right Nuclease
	         TCAGCCTTTTGCAGTTTAT
	5'-TATTT TCACCCTGTTGGGGTAAAT      GTTTTAAAGAGTGAGAAAAC      ATAAATTGAGAAAGGGTGA TAAAG-3'
	3'-ATAAA AGTGGGACAACCCCATTTA      CAAAATTTCTCACTCTTTTG      TATTTAACTCTTTCCCACT ATTTC-5'
	                                                            TATTTGACGTTTTCCGACT
	                                                                  Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 004-100789762
Forward Primer: 5'-GAGGCTTAGATGACTTCTGCAGG-3' Tm: 59.148
Reverse Primer: 5'-CTCTTGGGCCAACCCTATACAAC-3' Tm: 59.148
Expected PCR Product Length: 530
Expected CelI Cleavage Product Sizes: 316, 214
In Silico PCR
Genome Browser

Rank: 33	Homology Score: 10
	Genomic Region: Exon	Closest Gene: PLP1
Match Type: R14R
	          Right Nuclease
	         TCAGCCTTTTGCAGTTTAT
	5'-AGATT TCAGTCTTTTCCTGTTTTT         GTTAACTTTGGGTT         AAAAAAAAAAAAAGTCTGA TTGGT-3'
	3'-TCTAA AGTCAGAAAAGGACAAAAA         CAATTGAAACCCAA         TTTTTTTTTTTTTCAGACT AACCA-5'
	                                                            TATTTGACGTTTTCCGACT
	                                                                  Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 00X-103046628
Forward Primer: 5'-GGGTTGCATCAGAAATGTCTGGAG-3' Tm: 59.038
Reverse Primer: 5'-CCTTCTATGCAGCCTGGAAGC-3' Tm: 59.378
Expected PCR Product Length: 620
Expected CelI Cleavage Product Sizes: 416, 204
In Silico PCR
Genome Browser

Rank: 34	Homology Score: 10
	Genomic Region: Intron	Closest Gene: NAPEPLD
Match Type: R17R
	          Right Nuclease
	         TCAGCCTTTTGCAGTTTAT
	5'-CTAAC TCATAGTTATGAGGTTTAT       TCAACATCAAAACATGA        ACAAATTCCAAAATGCTGA AAGTC-3'
	3'-GATTG AGTATCAATACTCCAAATA       AGTTGTAGTTTTGTACT        TGTTTAAGGTTTTACGACT TTCAG-5'
	                                                            TATTTGACGTTTTCCGACT
	                                                                  Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 007-102753028
Forward Primer: 5'-CATCACACATTGGGGCCTGTC-3' Tm: 59.378
Reverse Primer: 5'-GAGATGGGGAAGATGAGAGAGG-3' Tm: 59.258
Expected PCR Product Length: 440
Expected CelI Cleavage Product Sizes: 270, 170
In Silico PCR
Genome Browser

Rank: 35	Homology Score: 10
	Genomic Region: Intron	Closest Gene: RIMS2
Match Type: L24L
	           Left Nuclease
	         TTTGTGGGCAACATGCTGG
	5'-ATGGA TATCTCTTCAACATACTGG    AGTCTTTAGGGTTTACTGTAGATA    AGATCATGTTGTCCACAAA CAGAG-3'
	3'-TACCT ATAGAGAAGTTGTATGACC    TCAGAAATCCCAAATGACATCTAT    TCTAGTACAACAGGTGTTT GTCTC-5'
	                                                            GGTCGTACAACGGGTGTTT
	                                                                   Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 008-104699899
Forward Primer: 5'-CATACCCCAGTCTCTGGTGATC-3' Tm: 59.258
Reverse Primer: 5'-CTCAACGGTGAAAAGTTGGAAACTTTCCTC-3' Tm: 60.058
Expected PCR Product Length: 542
Expected CelI Cleavage Product Sizes: 345, 197
In Silico PCR
Genome Browser

Rank: 36	Homology Score: 10
	Genomic Region: Intron	Closest Gene: PCID2
Match Type: R16R
	          Right Nuclease
	         TCAGCCTTTTGCAGTTTAT
	5'-ACAGA TCAGTATTTTGCAATTTAC        AACACTGCATTTGCCA        ATCATCATCAAGAAGCTGA AGATG-3'
	3'-TGTCT AGTCATAAAACGTTAAATG        TTGTGACGTAAACGGT        TAGTAGTAGTTCTTCGACT TCTAC-5'
	                                                            TATTTGACGTTTTCCGACT
	                                                                  Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 013-113857673
Forward Primer: 5'-AAATGGGTGCAAGACGATTTGGGC-3' Tm: 59.038
Reverse Primer: 5'-CTGGGCACCAGTGAGTTCTTG-3' Tm: 59.378
Expected PCR Product Length: 325
Expected CelI Cleavage Product Sizes: 200, 125
In Silico PCR
Genome Browser

Rank: 37	Homology Score: 10
	Genomic Region: Intron	Closest Gene: SAMD3
Match Type: L15L
	           Left Nuclease
	         TTTGTGGGCAACATGCTGG
	5'-AGGAG TTTTTGGGCGAGATGATGG        TGTTTTCTAAATATA         CAATCATGTCGTCTGCAAA CAGAG-3'
	3'-TCCTC AAAAACCCGCTCTACTACC        ACAAAAGATTTATAT         GTTAGTACAGCAGACGTTT GTCTC-5'
	                                                            GGTCGTACAACGGGTGTTT
	                                                                   Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 006-130491792
Forward Primer: 5'-GAAGAGGTCCTTCATGTCCCTTG-3' Tm: 59.148
Reverse Primer: 5'-GCCCTCTCTCACCACTCTTATTC-3' Tm: 59.148
Expected PCR Product Length: 358
Expected CelI Cleavage Product Sizes: 216, 142
In Silico PCR
Genome Browser

Rank: 38	Homology Score: 10
	Genomic Region: Intron	Closest Gene: LDB2
Match Type: R10L
	          Right Nuclease
	         TCAGCCTTTTGCAGTTTAT
	5'-GTCCC TTTGCCTTGTACAGTTTAT           TTTTATTTAC           CCAGTTTCTTGTTGACAAA TCATT-3'
	3'-CAGGG AAACGGAACATGTCAAATA           AAAATAAATG           GGTCAAAGAACAACTGTTT AGTAA-5'
	                                                            GGTCGTACAACGGGTGTTT
	                                                                   Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 004-016699419
Forward Primer: 5'-CACTCCAGTAGGGAAAGACTGATG-3' Tm: 59.038
Reverse Primer: 5'-GGGTGTGCAAATGGTGACATCG-3' Tm: 59.258
Expected PCR Product Length: 596
Expected CelI Cleavage Product Sizes: 365, 231
In Silico PCR
Genome Browser

Rank: 39	Homology Score: 10
	Genomic Region: Intron	Closest Gene: PARD3B
Match Type: R19L
	          Right Nuclease
	         TCAGCCTTTTGCAGTTTAT
	5'-AGATT TCAGTGTTGTGCAGTTTTT      TTGGCATCAGCTGAAAAGC       CCTATCTGATGCCCATAAA ATCTA-3'
	3'-TCTAA AGTCACAACACGTCAAAAA      AACCGTAGTCGACTTTTCG       GGATAGACTACGGGTATTT TAGAT-5'
	                                                            GGTCGTACAACGGGTGTTT
	                                                                   Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 002-205903727
Forward Primer: 5'-TCCTGCGGCGAAAGTGATCTC-3' Tm: 59.378
Reverse Primer: 5'-CACTAGTCAAGGCATCCAAACTCC-3' Tm: 59.038
Expected PCR Product Length: 327
Expected CelI Cleavage Product Sizes: 125, 202
In Silico PCR
Genome Browser

Rank: 40	Homology Score: 10
	Genomic Region: Intron	Closest Gene: SSH2
Match Type: L30R
	           Left Nuclease
	         TTTGTGGGCAACATGCTGG
	5'-CACTG TATGTGTGCAAAGAGGTGG AGTTCTGTTATTTCCTTACAGAATGAAGGT TTGAACAGCAAAAGGCAGA TATTT-3'
	3'-GTGAC ATACACACGTTTCTCCACC TCAAGACAATAAAGGAATGTCTTACTTCCA AACTTGTCGTTTTCCGTCT ATAAA-5'
	                                                            TATTTGACGTTTTCCGACT
	                                                                  Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 017-028194241
Forward Primer: 5'-CTCTGTGTGTGCTGAAAGCATGC-3' Tm: 59.148
Reverse Primer: 5'-CCCAGACAAATGTTCAAGGGATGC-3' Tm: 59.038
Expected PCR Product Length: 457
Expected CelI Cleavage Product Sizes: 326, 131
In Silico PCR
Genome Browser

Rank: 41	Homology Score: 10
	Genomic Region: Intron	Closest Gene: PDE1C
Match Type: L19L
	           Left Nuclease
	         TTTGTGGGCAACATGCTGG
	5'-GCCGG TTTGAAGGCATCAGGCTGG      ATGCCATAAAGGATGTAAA       CCACTTTTATCCCCACAAA GGGGA-3'
	3'-CGGCC AAACTTCCGTAGTCCGACC      TACGGTATTTCCTACATTT       GGTGAAAATAGGGGTGTTT CCCCT-5'
	                                                            GGTCGTACAACGGGTGTTT
	                                                                   Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 007-031822143
Forward Primer: 5'-ACCATGCGAAACGATACACCAGG-3' Tm: 59.148
Reverse Primer: 5'-GGGCAAAATCGCCTAACACAAAGC-3' Tm: 59.038
Expected PCR Product Length: 361
Expected CelI Cleavage Product Sizes: 125, 236
In Silico PCR
Genome Browser

Rank: 42	Homology Score: 10
	Genomic Region: Intron	Closest Gene: ZFAND3
Match Type: L18L
	           Left Nuclease
	         TTTGTGGGCAACATGCTGG
	5'-TAGCC TTTGTGGGCCACAAGCTCT       CTGTTGCAGCTACTCACC       CCTACATTGTGAACACAAA AGCAG-3'
	3'-ATCGG AAACACCCGGTGTTCGAGA       GACAACGTCGATGAGTGG       GGATGTAACACTTGTGTTT TCGTC-5'
	                                                            GGTCGTACAACGGGTGTTT
	                                                                   Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 006-037841403
Forward Primer: 5'-GTGAAGGTCAGCTGGAAGATGG-3' Tm: 59.258
Reverse Primer: 5'-GGAACAAACCCTGCCAACAAACC-3' Tm: 59.148
Expected PCR Product Length: 478
Expected CelI Cleavage Product Sizes: 169, 309
In Silico PCR
Genome Browser

Rank: 43	Homology Score: 10
	Genomic Region: Intron	Closest Gene: STIM1
Match Type: L23L
	           Left Nuclease
	         TTTGTGGGCAACATGCTGG
	5'-GTAGG TTTTTGGGAAAGGTGCTGG    TTAATTGACCTCAGGCCAGTGGT     GCAGCCTGGAGGCCCCAAA CCCTA-3'
	3'-CATCC AAAAACCCTTTCCACGACC    AATTAACTGGAGTCCGGTCACCA     CGTCGGACCTCCGGGGTTT GGGAT-5'
	                                                            GGTCGTACAACGGGTGTTT
	                                                                   Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 011-003985684
Forward Primer: 5'-TCTGATGCTAGCAGCAAAGGTCAC-3' Tm: 59.038
Reverse Primer: 5'-AACTCCAGCCTGGGCAACAGA-3' Tm: 59.378
Expected PCR Product Length: 326
Expected CelI Cleavage Product Sizes: 125, 201
In Silico PCR
Genome Browser

Rank: 44	Homology Score: 10
	Genomic Region: Intron	Closest Gene: TNRC6B
Match Type: R17R
	          Right Nuclease
	         TCAGCCTTTTGCAGTTTAT
	5'-AGAGA TCTGCTTTTTTAACTTTGT       CTTCACTCCATTCTGAT        ACCATCTGCTAAAGGCTGA TTTCA-3'
	3'-TCTCT AGACGAAAAAATTGAAACA       GAAGTGAGGTAAGACTA        TGGTAGACGATTTCCGACT AAAGT-5'
	                                                            TATTTGACGTTTTCCGACT
	                                                                  Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 022-040466169
Forward Primer: 5'-CGTGTTCCGTGTGCCTTTGTC-3' Tm: 59.378
Reverse Primer: 5'-CTGAAGTGCTGCCTCTGACC-3' Tm: 59.508
Expected PCR Product Length: 694
Expected CelI Cleavage Product Sizes: 309, 385
In Silico PCR
Genome Browser

Rank: 45	Homology Score: 10
	Genomic Region: Intron	Closest Gene: SLC14A2
Match Type: R15R
	          Right Nuclease
	         TCAGCCTTTTGCAGTTTAT
	5'-CTCCT TCAGCCTAAGGCATTTCAG        AATTCCACCAAGCTC         ATAGAATCCCAAAGGCTGA GTTTA-3'
	3'-GAGGA AGTCGGATTCCGTAAAGTC        TTAAGGTGGTTCGAG         TATCTTAGGGTTTCCGACT CAAAT-5'
	                                                            TATTTGACGTTTTCCGACT
	                                                                  Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 018-042963153
Forward Primer: 5'-CCAAGACCTAGGTCTCCTGATTC-3' Tm: 59.148
Reverse Primer: 5'-GTGAGTGGTTGCTCTGGGTAATC-3' Tm: 59.148
Expected PCR Product Length: 331
Expected CelI Cleavage Product Sizes: 204, 127
In Silico PCR
Genome Browser

Rank: 46	Homology Score: 10
	Genomic Region: Intron	Closest Gene: ADARB1
Match Type: L20R
	           Left Nuclease
	         TTTGTGGGCAACATGCTGG
	5'-ATTGG TGTGTGTGCAACTTGGTGG      TGGTGTTGGGGTTACTTTCC      AGAAGCTACTCAAGGCTTA GTCTC-3'
	3'-TAACC ACACACACGTTGAACCACC      ACCACAACCCCAATGAAAGG      TCTTCGATGAGTTCCGAAT CAGAG-5'
	                                                            TATTTGACGTTTTCCGACT
	                                                                  Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 021-046519527
Forward Primer: 5'-CTGGGTGACAGAGCGAGACT-3' Tm: 59.508
Reverse Primer: 5'-GCTTGGCTGGTTCAGTTCACAC-3' Tm: 59.258
Expected PCR Product Length: 437
Expected CelI Cleavage Product Sizes: 296, 141
In Silico PCR
Genome Browser

Rank: 47	Homology Score: 10
	Genomic Region: Intron	Closest Gene: KLHDC1
Match Type: R16L
	          Right Nuclease
	         TCAGCCTTTTGCAGTTTAT
	5'-AGCGC TCAGCCTTTTAAAATTTTT        TGTAGGGATGGGGATC        TCACTATGTTGCCCAGGCA GACCT-3'
	3'-TCGCG AGTCGGAAAATTTTAAAAA        ACATCCCTACCCCTAG        AGTGATACAACGGGTCCGT CTGGA-5'
	                                                            GGTCGTACAACGGGTGTTT
	                                                                   Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 014-050186827
Forward Primer: 5'-CCTGTGTAGCTGGGATTACAGGC-3' Tm: 61.088
Reverse Primer: 5'-AAGATGGAGGCCAGACTGGG-3' Tm: 59.508
Expected PCR Product Length: 325
Expected CelI Cleavage Product Sizes: 200, 125
In Silico PCR
Genome Browser

Rank: 48	Homology Score: 10
	Genomic Region: Intron	Closest Gene: DAB1
Match Type: L22R
	           Left Nuclease
	         TTTGTGGGCAACATGCTGG
	5'-TTACC TTTGTGAAGTACATGATAG     GTGATATATGTGGAGGTGTTTT     ATAAGCTGTAAAACACTGA ATAAA-3'
	3'-AATGG AAACACTTCATGTACTATC     CACTATATACACCTCCACAAAA     TATTCGACATTTTGTGACT TATTT-5'
	                                                            TATTTGACGTTTTCCGACT
	                                                                  Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 001-057601190
Forward Primer: 5'-GGGTTGCTTCTACCTTTTGGCTAC-3' Tm: 59.038
Reverse Primer: 5'-CTGTCAGTCCTGTTCATTTGGGG-3' Tm: 59.148
Expected PCR Product Length: 435
Expected CelI Cleavage Product Sizes: 301, 134
In Silico PCR
Genome Browser

Plate format Oligo Order Form
Well
Name
Sequence
5' mods
Internal
Notes
Primer Plate #1
A1
003-046414377F
GCACAGGGTGGAACAAGATGG
12212012SXJ93rlv_QUEUE_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
A2
003-046414377R
GTGACCGTCCTGGCTTTTAAAGCA
12212012SXJ93rlv_QUEUE_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
B1
003-046399009F
CATCCACAACATGCTGTCCACATC
12212012SXJ93rlv_QUEUE_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
B2
003-046399009R
CAAAGACCCACTCATTTGCAGCAG
12212012SXJ93rlv_QUEUE_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
C1
010-127381042F
CATCTCACGCAAAAAGACACCACC
12212012SXJ93rlv_QUEUE_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
C2
010-127381042R
GGACTCACAAGGACAGGCTC
12212012SXJ93rlv_QUEUE_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
D1
010-007305349F
CGTGCCACTAATGCCCAGGA
12212012SXJ93rlv_QUEUE_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
D2
010-007305349R
TTTGGCCTTCAGGTGGGACACT
12212012SXJ93rlv_QUEUE_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
E1
003-063153302F
TCTGGGCTGGGCTGTGCTTT
12212012SXJ93rlv_QUEUE_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
E2
003-063153302R
CCTAGTTGCTCCTTAGTACTTCCC
12212012SXJ93rlv_QUEUE_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
F1
004-008527974F
GCTCAAGTGTGAGGGTGCTG
12212012SXJ93rlv_QUEUE_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
F2
004-008527974R
TAAGGAGGCTGGAGGGAGCA
12212012SXJ93rlv_QUEUE_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
G1
002-116474320F
CATTTGTGGCCAAAGGTGCCTTGA
12212012SXJ93rlv_QUEUE_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
G2
002-116474320R
CCCTCCCTCTCATTCATCCAAAG
12212012SXJ93rlv_QUEUE_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
H1
002-160206641F
CAGGAGGCATCTTAGCTACTTCCA
12212012SXJ93rlv_QUEUE_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
H2
002-160206641R
AATTCACGCCATTGCACTCCAGC
12212012SXJ93rlv_QUEUE_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
A3
003-157066450F
AAGGAAGAGGGGGCTGCAGA
12212012SXJ93rlv_QUEUE_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
A4
003-157066450R
GCTGAGTCCTTGTCTGGTTTTGG
12212012SXJ93rlv_QUEUE_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
B3
004-020877272F
GCCTAGGTCAGAGCCTTTAGC
12212012SXJ93rlv_QUEUE_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
B4
004-020877272R
GCACTATCTCAGTGCCCTCATTC
12212012SXJ93rlv_QUEUE_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
C3
022-021114662F
GTGCCCTTACACTAAAACTGGCC
12212012SXJ93rlv_QUEUE_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
C4
022-021114662R
CTGGGAAGACAGAGAAATGGTGG
12212012SXJ93rlv_QUEUE_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
D3
011-076680948F
CCTGCATATTGTGCACATGTACCC
12212012SXJ93rlv_QUEUE_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
D4
011-076680948R
GGATGAACCCCTCTTGGCCA
12212012SXJ93rlv_QUEUE_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
E3
003-089353684F
GTTCACTAGGCCAGGAACAGTC
12212012SXJ93rlv_QUEUE_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
E4
003-089353684R
GCATGGCTAAAGAAGGCCAACATC
12212012SXJ93rlv_QUEUE_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
F3
002-105729897F
TGAGTGGACTCCCTCTTGGC
12212012SXJ93rlv_QUEUE_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
F4
002-105729897R
GGTGGATGAGCTGCAACTAGTTC
12212012SXJ93rlv_QUEUE_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
G3
019-012111242F
GTAGTGTCGTCTCATGGGCAAAAG
12212012SXJ93rlv_QUEUE_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
G4
019-012111242R
CAGTCCTGAGAGTGTGTTTCCTTG
12212012SXJ93rlv_QUEUE_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
H3
003-177863333F
CAGTCTGGAGACCTCCAGTAG
12212012SXJ93rlv_QUEUE_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
H4
003-177863333R
GTCCTTCCAAGGCATCACCATTTC
12212012SXJ93rlv_QUEUE_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
A5
001-231235899F
CTTGAGGCCAGTGCTTGTTTAGG
12212012SXJ93rlv_QUEUE_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
A6
001-231235899R
TCCCCCTCTTGGCCAAAAGC
12212012SXJ93rlv_QUEUE_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
B5
012-042468830F
GAATGGACACCTACCTTGGCC
12212012SXJ93rlv_QUEUE_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
B6
012-042468830R
GTCTGGCAATGGATCAGCTGCA
12212012SXJ93rlv_QUEUE_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
C5
004-069131937F
ATGGCCTGTGGGTGAGGCTT
12212012SXJ93rlv_QUEUE_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
C6
004-069131937R
GGAGAATCACTTGAACCCGGG
12212012SXJ93rlv_QUEUE_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
D5
009-090818650F
CTGCAAGGACTCAAAAAATTTGACCCCTAC
12212012SXJ93rlv_QUEUE_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
D6
009-090818650R
TCACTATATGGGCCAGGCTGATC
12212012SXJ93rlv_QUEUE_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
E5
006-136890071F
GAAGAACCACCTACCCCCAG
12212012SXJ93rlv_QUEUE_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
E6
006-136890071R
CTACAGAATGCAGATTTCCCCCAC
12212012SXJ93rlv_QUEUE_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
F5
012-004929936F
GGGAGGACTAATGACTGAGGG
12212012SXJ93rlv_QUEUE_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
F6
012-004929936R
CTCACCCTGGTCCTGTGATTTG
12212012SXJ93rlv_QUEUE_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
G5
006-056297715F
TCTACTCTCTTGGTGTCGCCATG
12212012SXJ93rlv_QUEUE_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
G6
006-056297715R
AGTGGTGACTAGGACCGCGT
12212012SXJ93rlv_QUEUE_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
H5
008-077661832F
GGGCCAGACATTTCTTTAGCCTAG
12212012SXJ93rlv_QUEUE_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
H6
008-077661832R
GGAGACATCCTCAGGGAAAGC
12212012SXJ93rlv_QUEUE_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
A7
005-112991631F
TACTCCAGCCTGTGTGACAAGAGC
12212012SXJ93rlv_QUEUE_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
A8
005-112991631R
CTACATGGATCTCCTCTTGGCC
12212012SXJ93rlv_QUEUE_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
B7
006-114639685F
CCCACTAAGGCTCTTCACAGCA
12212012SXJ93rlv_QUEUE_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
B8
006-114639685R
TAGGCATTGAGCACTTGCTAGGTC
12212012SXJ93rlv_QUEUE_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
C7
005-164243933F
CAGTAGACAATACGTAATACCTCCTGCAAG
12212012SXJ93rlv_QUEUE_Ranking-Homology_5T	Warning! Primer pair was generated using suboptimal parameters. 
C8
005-164243933R
CTGCCCTACTCCATTTTAAAGATATTCTCCC
12212012SXJ93rlv_QUEUE_Ranking-Homology_5T	Warning! Primer pair was generated using suboptimal parameters. 
D7
00Y-022149362F
CATGACTGACACCCTCCACG
12212012SXJ93rlv_QUEUE_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
D8
00Y-022149362R
AGCAAAGCAGGTGGCACAGC
12212012SXJ93rlv_QUEUE_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
E7
013-064120919F
CTCCTAGTCTACAAACCTCTGCAG
12212012SXJ93rlv_QUEUE_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
E8
013-064120919R
CACCCTTAGCAAATAGGCAGAGAG
12212012SXJ93rlv_QUEUE_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
F7
011-080866146F
CCCAGAAACCACCAACTAGCATG
12212012SXJ93rlv_QUEUE_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
F8
011-080866146R
CTGGAGATCTGAGACCATGCG
12212012SXJ93rlv_QUEUE_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
G7
012-089421427F
GCATTCACTCGCCTGTTCCTC
12212012SXJ93rlv_QUEUE_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
G8
012-089421427R
GCTGACTTAAGAGGGCTCTTTGC
12212012SXJ93rlv_QUEUE_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
H7
004-100789762F
GAGGCTTAGATGACTTCTGCAGG
12212012SXJ93rlv_QUEUE_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
H8
004-100789762R
CTCTTGGGCCAACCCTATACAAC
12212012SXJ93rlv_QUEUE_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
A9
00X-103046628F
GGGTTGCATCAGAAATGTCTGGAG
12212012SXJ93rlv_QUEUE_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
A10
00X-103046628R
CCTTCTATGCAGCCTGGAAGC
12212012SXJ93rlv_QUEUE_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
B9
007-102753028F
CATCACACATTGGGGCCTGTC
12212012SXJ93rlv_QUEUE_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
B10
007-102753028R
GAGATGGGGAAGATGAGAGAGG
12212012SXJ93rlv_QUEUE_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
C9
008-104699899F
CATACCCCAGTCTCTGGTGATC
12212012SXJ93rlv_QUEUE_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
C10
008-104699899R
CTCAACGGTGAAAAGTTGGAAACTTTCCTC
12212012SXJ93rlv_QUEUE_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
D9
013-113857673F
AAATGGGTGCAAGACGATTTGGGC
12212012SXJ93rlv_QUEUE_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
D10
013-113857673R
CTGGGCACCAGTGAGTTCTTG
12212012SXJ93rlv_QUEUE_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
E9
006-130491792F
GAAGAGGTCCTTCATGTCCCTTG
12212012SXJ93rlv_QUEUE_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
E10
006-130491792R
GCCCTCTCTCACCACTCTTATTC
12212012SXJ93rlv_QUEUE_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
F9
004-016699419F
CACTCCAGTAGGGAAAGACTGATG
12212012SXJ93rlv_QUEUE_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
F10
004-016699419R
GGGTGTGCAAATGGTGACATCG
12212012SXJ93rlv_QUEUE_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
G9
002-205903727F
TCCTGCGGCGAAAGTGATCTC
12212012SXJ93rlv_QUEUE_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
G10
002-205903727R
CACTAGTCAAGGCATCCAAACTCC
12212012SXJ93rlv_QUEUE_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
H9
017-028194241F
CTCTGTGTGTGCTGAAAGCATGC
12212012SXJ93rlv_QUEUE_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
H10
017-028194241R
CCCAGACAAATGTTCAAGGGATGC
12212012SXJ93rlv_QUEUE_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
A11
007-031822143F
ACCATGCGAAACGATACACCAGG
12212012SXJ93rlv_QUEUE_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
A12
007-031822143R
GGGCAAAATCGCCTAACACAAAGC
12212012SXJ93rlv_QUEUE_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
B11
006-037841403F
GTGAAGGTCAGCTGGAAGATGG
12212012SXJ93rlv_QUEUE_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
B12
006-037841403R
GGAACAAACCCTGCCAACAAACC
12212012SXJ93rlv_QUEUE_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
C11
011-003985684F
TCTGATGCTAGCAGCAAAGGTCAC
12212012SXJ93rlv_QUEUE_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
C12
011-003985684R
AACTCCAGCCTGGGCAACAGA
12212012SXJ93rlv_QUEUE_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
D11
022-040466169F
CGTGTTCCGTGTGCCTTTGTC
12212012SXJ93rlv_QUEUE_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
D12
022-040466169R
CTGAAGTGCTGCCTCTGACC
12212012SXJ93rlv_QUEUE_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
E11
018-042963153F
CCAAGACCTAGGTCTCCTGATTC
12212012SXJ93rlv_QUEUE_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
E12
018-042963153R
GTGAGTGGTTGCTCTGGGTAATC
12212012SXJ93rlv_QUEUE_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
F11
021-046519527F
CTGGGTGACAGAGCGAGACT
12212012SXJ93rlv_QUEUE_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
F12
021-046519527R
GCTTGGCTGGTTCAGTTCACAC
12212012SXJ93rlv_QUEUE_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
G11
014-050186827F
CCTGTGTAGCTGGGATTACAGGC
12212012SXJ93rlv_QUEUE_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
G12
014-050186827R
AAGATGGAGGCCAGACTGGG
12212012SXJ93rlv_QUEUE_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
H11
001-057601190F
GGGTTGCTTCTACCTTTTGGCTAC
12212012SXJ93rlv_QUEUE_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
H12
001-057601190R
CTGTCAGTCCTGTTCATTTGGGG
12212012SXJ93rlv_QUEUE_Ranking-Homology_5T	Primer pair generated using optimal parameters.