PROGNOS Summary for 12212012SXJ93rlv_QUEUE_Ranking-Homology

The hg19 genome was searched for the TALEN pair targeting:
5'- TTTGTGGGCAACATGCTGG NN..NN ATAAACTGCAAAAGGCTGA -3'

Up to 6 mismatches were allowed in each nuclease half-site.

Allowed spacing distances were: 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30

Homodimers and heterodimers of the nucleases were included in the output

4684 total sites were located
Mismatch Types
Genomic Regions
  0-0: 1
  0-1: 1
  3-5: 5
  4-4: 2
  3-6: 21
  4-5: 13
  4-6: 121
  5-5: 91
  5-6: 1014
  6-6: 3415
  Exons: 120
  Promoters: 33
  Introns: 1825
  Intergenic: 2706


The top 48 sites are ranked below according to a weighted sequence homology.
For the full output, click here.

This output will be deleted from the server one week after it is generated.  Please save your results.

Primer pairs were designed using the 422 bp of sequence on either side of the cleavage site to melt close to 60 °C

For easy visualization of cleavage on gels:
The smallest fragment will be longer than 125 bp, which is also the minimum separation distance between uncleaved and cleaved products.
The two cleavage products will be separated by a minimum size of 75 bp
Rank: 1	Homology Score: 98
	Genomic Region: Exon	Closest Gene: CCR5
Match Type: L14R
	           Left Nuclease
	         TTTGTGGGCAACATGCTGG
	5'-TTGGT TTTGTGGGCAACATGCTGG         TCATCCTCATCCTG         ATAAACTGCAAAAGGCTGA AGAGC-3'
	3'-AACCA AAACACCCGTTGTACGACC         AGTAGGAGTAGGAC         TATTTGACGTTTTCCGACT TCTCG-5'
	                                                            TATTTGACGTTTTCCGACT
	                                                                  Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 003-046414377
Forward Primer: 5'-GCACAGGGTGGAACAAGATGG-3' Tm: 59.378
Reverse Primer: 5'-GTGACCGTCCTGGCTTTTAAAGCA-3' Tm: 59.038
Expected PCR Product Length: 446
Expected CelI Cleavage Product Sizes: 177, 269
In Silico PCR
Genome Browser

Rank: 2	Homology Score: 85
	Genomic Region: Exon	Closest Gene: CCR2
Match Type: L14R
	           Left Nuclease
	         TTTGTGGGCAACATGCTGG
	5'-TTGGT TTTGTGGGCAACATGCTGG         TCGTCCTCATCTTA         ATAAACTGCAAAAAGCTGA AGTGC-3'
	3'-AACCA AAACACCCGTTGTACGACC         AGCAGGAGTAGAAT         TATTTGACGTTTTTCGACT TCACG-5'
	                                                            TATTTGACGTTTTCCGACT
	                                                                  Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 003-046399009
Forward Primer: 5'-CATCCACAACATGCTGTCCACATC-3' Tm: 59.038
Reverse Primer: 5'-CAAAGACCCACTCATTTGCAGCAG-3' Tm: 59.038
Expected PCR Product Length: 336
Expected CelI Cleavage Product Sizes: 206, 130
In Silico PCR
Genome Browser

Rank: 3	Homology Score: 20
	Genomic Region: Intron	Closest Gene: LOC283038
Match Type: L17L
	           Left Nuclease
	         TTTGTGGGCAACATGCTGG
	5'-TCCTT TTTGAGGGGAAGATGCTGG       GTTTATTTAAAACACAT        GCAACATGTTGGACACACA CACAC-3'
	3'-AGGAA AAACTCCCCTTCTACGACC       CAAATAAATTTTGTGTA        CGTTGTACAACCTGTGTGT GTGTG-5'
	                                                            GGTCGTACAACGGGTGTTT
	                                                                   Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 010-127381042
Forward Primer: 5'-CATCTCACGCAAAAAGACACCACC-3' Tm: 59.038
Reverse Primer: 5'-GGACTCACAAGGACAGGCTC-3' Tm: 59.508
Expected PCR Product Length: 479
Expected CelI Cleavage Product Sizes: 282, 197
In Silico PCR
Genome Browser

Rank: 4	Homology Score: 20
	Genomic Region: Intron	Closest Gene: PKP2
Match Type: L15R
	           Left Nuclease
	         TTTGTGGGCAACATGCTGG
	5'-GTATA TCTGTGGGAAAAAAGGTGG        TAATGAAGGTTGTGG         ATAGACTGAAAAAGGCTGT GTATG-3'
	3'-CATAT AGACACCCTTTTTTCCACC        ATTACTTCCAACACC         TATCTGACTTTTTCCGACA CATAC-5'
	                                                            TATTTGACGTTTTCCGACT
	                                                                  Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 012-032991814
Forward Primer: 5'-GCTTTTGCTGGGGAAGCTGG-3' Tm: 59.508
Reverse Primer: 5'-CAAGCCTGGATCAGATACCTCCT-3' Tm: 59.148
Expected PCR Product Length: 390
Expected CelI Cleavage Product Sizes: 236, 154
In Silico PCR
Genome Browser

Rank: 5	Homology Score: 20
	Genomic Region: Intron	Closest Gene: SFMBT2
Match Type: L11L
	           Left Nuclease
	         TTTGTGGGCAACATGCTGG
	5'-AGCCA TTTGGGGATAACATGATGA          GAAGGAGATAT           CCATCATGTATCCCACAAA CATTA-3'
	3'-TCGGT AAACCCCTATTGTACTACT          CTTCCTCTATA           GGTAGTACATAGGGTGTTT GTAAT-5'
	                                                            GGTCGTACAACGGGTGTTT
	                                                                   Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 010-007305349
Forward Primer: 5'-CGTGCCACTAATGCCCAGGA-3' Tm: 59.508
Reverse Primer: 5'-TTTGGCCTTCAGGTGGGACACT-3' Tm: 59.258
Expected PCR Product Length: 329
Expected CelI Cleavage Product Sizes: 203, 126
In Silico PCR
Genome Browser

Rank: 6	Homology Score: 20
	Genomic Region: Intergenic	Closest Gene: LOC285401
Match Type: R12R
	          Right Nuclease
	         TCAGCCTTTTGCAGTTTAT
	5'-ACTCC TCAGCCTGTTTCAGTTTTT          AAAATATGTATT          ATAAAATTCATAATGCTAA GTCAC-3'
	3'-TGAGG AGTCGGACAAAGTCAAAAA          TTTTATACATAA          TATTTTAAGTATTACGATT CAGTG-5'
	                                                            TATTTGACGTTTTCCGACT
	                                                                  Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 003-063153302
Forward Primer: 5'-TCTGGGCTGGGCTGTGCTTT-3' Tm: 59.508
Reverse Primer: 5'-CCTAGTTGCTCCTTAGTACTTCCC-3' Tm: 59.038
Expected PCR Product Length: 340
Expected CelI Cleavage Product Sizes: 126, 214
In Silico PCR
Genome Browser

Rank: 7	Homology Score: 20
	Genomic Region: Intergenic	Closest Gene: TRMT44
Match Type: L22L
	           Left Nuclease
	         TTTGTGGGCAACATGCTGG
	5'-TCCAG TTTGAGGGGGAAATGCTTG     GGTATGAGCTCCCTCCCAGGAC     CCAGCGTGGTGCCCACACA TCATG-3'
	3'-AGGTC AAACTCCCCCTTTACGAAC     CCATACTCGAGGGAGGGTCCTG     GGTCGCACCACGGGTGTGT AGTAC-5'
	                                                            GGTCGTACAACGGGTGTTT
	                                                                   Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 004-008527974
Forward Primer: 5'-GCTCAAGTGTGAGGGTGCTG-3' Tm: 59.508
Reverse Primer: 5'-TAAGGAGGCTGGAGGGAGCA-3' Tm: 59.508
Expected PCR Product Length: 341
Expected CelI Cleavage Product Sizes: 208, 133
In Silico PCR
Genome Browser

Rank: 8	Homology Score: 18
	Genomic Region: Intron	Closest Gene: DPP10
Match Type: R18R
	          Right Nuclease
	         TCAGCCTTTTGCAGTTTAT
	5'-CACCT TCTGCATTTTTCAGTTTGT       TTTCTTATCAGCCTGCCC       ATAAACTGGAAAAGCATTA GGCAC-3'
	3'-GTGGA AGACGTAAAAAGTCAAACA       AAAGAATAGTCGGACGGG       TATTTGACCTTTTCGTAAT CCGTG-5'
	                                                            TATTTGACGTTTTCCGACT
	                                                                  Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 002-116474320
Forward Primer: 5'-CATTTGTGGCCAAAGGTGCCTTGA-3' Tm: 59.038
Reverse Primer: 5'-CCCTCCCTCTCATTCATCCAAAG-3' Tm: 59.148
Expected PCR Product Length: 361
Expected CelI Cleavage Product Sizes: 129, 232
In Silico PCR
Genome Browser

Rank: 9	Homology Score: 18
	Genomic Region: Intron	Closest Gene: LOC100133091
Match Type: R25L
	          Right Nuclease
	         TCAGCCTTTTGCAGTTTAT
	5'-ATCTT TCAGCTTTCATCAGTTTAT   GCCTAATCCTGCTCAGTAGGTGCCA    CCCCCATGTTGCCCACGAG GACTG-3'
	3'-TAGAA AGTCGAAAGTAGTCAAATA   CGGATTAGGACGAGTCATCCACGGT    GGGGGTACAACGGGTGCTC CTGAC-5'
	                                                            GGTCGTACAACGGGTGTTT
	                                                                   Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 007-076237892
Forward Primer: 5'-ACATGTCTGTACCCGAGTCAACAC-3' Tm: 59.038
Reverse Primer: 5'-ACTTGTGCCCCCTTTGCTGG-3' Tm: 59.508
Expected PCR Product Length: 328
Expected CelI Cleavage Product Sizes: 125, 203
In Silico PCR
Genome Browser

Rank: 10	Homology Score: 17
	Genomic Region: Exon	Closest Gene: ADORA3
Match Type: L24L
	           Left Nuclease
	         TTTGTGGGCAACATGCTGG
	5'-AGAAG GTGGTGGTCTGCAGGCTGG    GGTTCAGCTTGACCACGCAGATGA    CCAGCACGTTGCCCACTAT GGCGC-3'
	3'-TCTTC CACCACCAGACGTCCGACC    CCAAGTCGAACTGGTGCGTCTACT    GGTCGTGCAACGGGTGATA CCGCG-5'
	                                                            GGTCGTACAACGGGTGTTT
	                                                                   Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 001-112045670
Forward Primer: 5'-CCAGCAAGGACATGATGGAGG-3' Tm: 59.378
Reverse Primer: 5'-TCCCCTGGGAAGGCAAGATG-3' Tm: 59.508
Expected PCR Product Length: 325
Expected CelI Cleavage Product Sizes: 200, 125
In Silico PCR
Genome Browser

Rank: 11	Homology Score: 17
	Genomic Region: Exon	Closest Gene: BAZ2B
Match Type: R29R
	          Right Nuclease
	         TCAGCCTTTTGCAGTTTAT
	5'-CACTT TCTGCCTTTTTCAGTTTTT CTCTTTCTTTTGCAATTTCTTCTAGTCCT  ACAAAATGAAAAAGCATTA TGAAC-3'
	3'-GTGAA AGACGGAAAAAGTCAAAAA GAGAAAGAAAACGTTAAAGAAGATCAGGA  TGTTTTACTTTTTCGTAAT ACTTG-5'
	                                                            TATTTGACGTTTTCCGACT
	                                                                  Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 002-160206641
Forward Primer: 5'-CAGGAGGCATCTTAGCTACTTCCA-3' Tm: 59.038
Reverse Primer: 5'-AATTCACGCCATTGCACTCCAGC-3' Tm: 59.148
Expected PCR Product Length: 510
Expected CelI Cleavage Product Sizes: 217, 293
In Silico PCR
Genome Browser

Rank: 12	Homology Score: 17
	Genomic Region: Intron	Closest Gene: VEPH1
Match Type: R29L
	          Right Nuclease
	         TCAGCCTTTTGCAGTTTAT
	5'-TCCAG TCAAATTTTAGCTATTTAT CCATTGCTGTGGTCCTCTGACCCCTTGGG  CCAGCAGGTTGGCCACATA GGCAC-3'
	3'-AGGTC AGTTTAAAATCGATAAATA GGTAACGACACCAGGAGACTGGGGAACCC  GGTCGTCCAACCGGTGTAT CCGTG-5'
	                                                            GGTCGTACAACGGGTGTTT
	                                                                   Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 003-157066450
Forward Primer: 5'-AAGGAAGAGGGGGCTGCAGA-3' Tm: 59.508
Reverse Primer: 5'-GCTGAGTCCTTGTCTGGTTTTGG-3' Tm: 59.148
Expected PCR Product Length: 388
Expected CelI Cleavage Product Sizes: 153, 235
In Silico PCR
Genome Browser

Rank: 13	Homology Score: 17
	Genomic Region: Intron	Closest Gene: CABLES1
Match Type: R14R
	          Right Nuclease
	         TCAGCCTTTTGCAGTTTAT
	5'-CACAT GCCTCTTTTTGCAGTTTCA         TTAGCTCATTCAAA         ATATACTGTAAAAGGCTAA CAAGA-3'
	3'-GTGTA CGGAGAAAAACGTCAAAGT         AATCGAGTAAGTTT         TATATGACATTTTCCGATT GTTCT-5'
	                                                            TATTTGACGTTTTCCGACT
	                                                                  Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 018-020747199
Forward Primer: 5'-CTTTCCAGGCTGCTTTGGGAG-3' Tm: 59.378
Reverse Primer: 5'-CTCCCCCCAATCCCCCAAAA-3' Tm: 59.508
Expected PCR Product Length: 325
Expected CelI Cleavage Product Sizes: 200, 125
In Silico PCR
Genome Browser

Rank: 14	Homology Score: 17
	Genomic Region: Intron	Closest Gene: KCNIP4
Match Type: L30R
	           Left Nuclease
	         TTTGTGGGCAACATGCTGG
	5'-TATTT TTAATGGGCAACATGTTGG CCATTCAAGTTGCTTTTTGCAAACAAATCC TCCAACAGCCAAATGCTGA TGGTT-3'
	3'-ATAAA AATTACCCGTTGTACAACC GGTAAGTTCAACGAAAAACGTTTGTTTAGG AGGTTGTCGGTTTACGACT ACCAA-5'
	                                                            TATTTGACGTTTTCCGACT
	                                                                  Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 004-020877272
Forward Primer: 5'-GCCTAGGTCAGAGCCTTTAGC-3' Tm: 59.378
Reverse Primer: 5'-GCACTATCTCAGTGCCCTCATTC-3' Tm: 59.148
Expected PCR Product Length: 483
Expected CelI Cleavage Product Sizes: 144, 339
In Silico PCR
Genome Browser

Rank: 15	Homology Score: 17
	Genomic Region: Intron	Closest Gene: PI4KA
Match Type: R26R
	          Right Nuclease
	         TCAGCCTTTTGCAGTTTAT
	5'-TAATA TTAGCCTTTTGCAATTTAA   AAAAATTAAGGTATAATTTGCATATA   ATAAAATCCACAGGTCTTA TGTGC-3'
	3'-ATTAT AATCGGAAAACGTTAAATT   TTTTTAATTCCATATTAAACGTATAT   TATTTTAGGTGTCCAGAAT ACACG-5'
	                                                            TATTTGACGTTTTCCGACT
	                                                                  Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 022-021114662
Forward Primer: 5'-GTGCCCTTACACTAAAACTGGCC-3' Tm: 59.148
Reverse Primer: 5'-CTGGGAAGACAGAGAAATGGTGG-3' Tm: 59.148
Expected PCR Product Length: 398
Expected CelI Cleavage Product Sizes: 150, 248
In Silico PCR
Genome Browser

Rank: 16	Homology Score: 17
	Genomic Region: Intron	Closest Gene: NCOA5
Match Type: L16R
	           Left Nuclease
	         TTTGTGGGCAACATGCTGG
	5'-AAAAT AATCTAGAAAACATGCTGG        AAATGGCAGAACCATT        ATAAACTACAAAAGGATGG GGTGA-3'
	3'-TTTTA TTAGATCTTTTGTACGACC        TTTACCGTCTTGGTAA        TATTTGATGTTTTCCTACC CCACT-5'
	                                                            TATTTGACGTTTTCCGACT
	                                                                  Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 020-044711346
Forward Primer: 5'-GTGTCCAAGTGGTGGTCTGAAAAC-3' Tm: 59.038
Reverse Primer: 5'-CCGCTCTTGTTATTCCTAGTCCTC-3' Tm: 59.038
Expected PCR Product Length: 419
Expected CelI Cleavage Product Sizes: 129, 290
In Silico PCR
Genome Browser

Rank: 17	Homology Score: 17
	Genomic Region: Intron	Closest Gene: ACER3
Match Type: R27R
	          Right Nuclease
	         TCAGCCTTTTGCAGTTTAT
	5'-TTTTG TCAGTCTTATGATCTCTAT  TTTAACCTTAATCATGGGCAGTTGTGC   CTAAACTCCAAAAGGGTGA GGATT-3'
	3'-AAAAC AGTCAGAATACTAGAGATA  AAATTGGAATTAGTACCCGTCAACACG   GATTTGAGGTTTTCCCACT CCTAA-5'
	                                                            TATTTGACGTTTTCCGACT
	                                                                  Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 011-076680948
Forward Primer: 5'-CCTGCATATTGTGCACATGTACCC-3' Tm: 59.038
Reverse Primer: 5'-GGATGAACCCCTCTTGGCCA-3' Tm: 59.508
Expected PCR Product Length: 355
Expected CelI Cleavage Product Sizes: 224, 131
In Silico PCR
Genome Browser

Rank: 18	Homology Score: 17
	Genomic Region: Intron	Closest Gene: EPHA3
Match Type: R20R
	          Right Nuclease
	         TCAGCCTTTTGCAGTTTAT
	5'-TCCTC TCTCCCTTTTCCTGTTTTC      TGCTCACTGCATACTACAAC      ATAAACTCCAAAAGGGAGA GGTTC-3'
	3'-AGGAG AGAGGGAAAAGGACAAAAG      ACGAGTGACGTATGATGTTG      TATTTGAGGTTTTCCCTCT CCAAG-5'
	                                                            TATTTGACGTTTTCCGACT
	                                                                  Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 003-089353684
Forward Primer: 5'-GTTCACTAGGCCAGGAACAGTC-3' Tm: 59.258
Reverse Primer: 5'-GCATGGCTAAAGAAGGCCAACATC-3' Tm: 59.038
Expected PCR Product Length: 460
Expected CelI Cleavage Product Sizes: 268, 192
In Silico PCR
Genome Browser

Rank: 19	Homology Score: 17
	Genomic Region: Intergenic	Closest Gene: MRPS9
Match Type: R27R
	          Right Nuclease
	         TCAGCCTTTTGCAGTTTAT
	5'-TACCT TCTCCCTTTTGCAGTTTAG  ACAAAACTGCCCGGCATTAATGTTATA   ATAGAGACCATAAGACTGA CAGAA-3'
	3'-ATGGA AGAGGGAAAACGTCAAATC  TGTTTTGACGGGCCGTAATTACAATAT   TATCTCTGGTATTCTGACT GTCTT-5'
	                                                            TATTTGACGTTTTCCGACT
	                                                                  Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 002-105729897
Forward Primer: 5'-TGAGTGGACTCCCTCTTGGC-3' Tm: 59.508
Reverse Primer: 5'-GGTGGATGAGCTGCAACTAGTTC-3' Tm: 59.148
Expected PCR Product Length: 351
Expected CelI Cleavage Product Sizes: 132, 219
In Silico PCR
Genome Browser

Rank: 20	Homology Score: 17
	Genomic Region: Intergenic	Closest Gene: AP1AR
Match Type: R18L
	          Right Nuclease
	         TCAGCCTTTTGCAGTTTAT
	5'-AATTT TCAGCTTTTTCCTGTTTAT       CGTGATACGAATATTCCA       ACAGCATGTTAAACAAAAG ACGTT-3'
	3'-TTAAA AGTCGAAAAAGGACAAATA       GCACTATGCTTATAAGGT       TGTCGTACAATTTGTTTTC TGCAA-5'
	                                                            GGTCGTACAACGGGTGTTT
	                                                                   Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 004-113138626
Forward Primer: 5'-GCCATCCAAATGTGGACAGTAGG-3' Tm: 59.148
Reverse Primer: 5'-GTAGGGGAAACCAATTACAGTGCC-3' Tm: 59.038
Expected PCR Product Length: 394
Expected CelI Cleavage Product Sizes: 147, 247
In Silico PCR
Genome Browser

Rank: 21	Homology Score: 17
	Genomic Region: Intergenic	Closest Gene: ZNF433
Match Type: R26L
	          Right Nuclease
	         TCAGCCTTTTGCAGTTTAT
	5'-CCCAT TCAGCCTTCAGCAGTTCAT   CAATTACAATTTAAGTTTTTCTACCA   CAATACTGTTTCTCACAAA GGTTT-3'
	3'-GGGTA AGTCGGAAGTCGTCAAGTA   GTTAATGTTAAATTCAAAAAGATGGT   GTTATGACAAAGAGTGTTT CCAAA-5'
	                                                            GGTCGTACAACGGGTGTTT
	                                                                   Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 019-012111242
Forward Primer: 5'-GTAGTGTCGTCTCATGGGCAAAAG-3' Tm: 59.038
Reverse Primer: 5'-CAGTCCTGAGAGTGTGTTTCCTTG-3' Tm: 59.038
Expected PCR Product Length: 365
Expected CelI Cleavage Product Sizes: 135, 230
In Silico PCR
Genome Browser

Rank: 22	Homology Score: 17
	Genomic Region: Intergenic	Closest Gene: MIR563
Match Type: R28R
	          Right Nuclease
	         TCAGCCTTTTGCAGTTTAT
	5'-GCATT CTAACCAATTGCAATTTAT  GAACCAATTTGTTTTCCAATTTGAATAA  ATAAACTGAAAAAGTTTGA GACAG-3'
	3'-CGTAA GATTGGTTAACGTTAAATA  CTTGGTTAAACAAAAGGTTAAACTTATT  TATTTGACTTTTTCAAACT CTGTC-5'
	                                                            TATTTGACGTTTTCCGACT
	                                                                  Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 003-015921847
Forward Primer: 5'-GCTCTAGAACTCTAGACTGTGGG-3' Tm: 59.148
Reverse Primer: 5'-CTCACGGCCAATCCTGTTTTATCC-3' Tm: 59.038
Expected PCR Product Length: 412
Expected CelI Cleavage Product Sizes: 165, 247
In Silico PCR
Genome Browser

Rank: 23	Homology Score: 17
	Genomic Region: Intergenic	Closest Gene: KCNMB2-IT1
Match Type: R27R
	          Right Nuclease
	         TCAGCCTTTTGCAGTTTAT
	5'-AAATT TCAGTATTTTGCATTTTAT  GACTCAAATGTTATGCTATTTGGGATT   CTAAACTTCAAAATCCCCA CAAAT-3'
	3'-TTTAA AGTCATAAAACGTAAAATA  CTGAGTTTACAATACGATAAACCCTAA   GATTTGAAGTTTTAGGGGT GTTTA-5'
	                                                            TATTTGACGTTTTCCGACT
	                                                                  Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 003-177863333
Forward Primer: 5'-CAGTCTGGAGACCTCCAGTAG-3' Tm: 59.378
Reverse Primer: 5'-GTCCTTCCAAGGCATCACCATTTC-3' Tm: 59.038
Expected PCR Product Length: 355
Expected CelI Cleavage Product Sizes: 137, 218
In Silico PCR
Genome Browser

Rank: 24	Homology Score: 17
	Genomic Region: Intergenic	Closest Gene: FAM89A
Match Type: R29R
	          Right Nuclease
	         TCAGCCTTTTGCAGTTTAT
	5'-TTCTG TCAGTCTTATGATCTCTAT TTAACATTAATGCTGGTTAGTCATTGTGT  CTAAACTGCAAAAGGGAGA GGTTA-3'
	3'-AAGAC AGTCAGAATACTAGAGATA AATTGTAATTACGACCAATCAGTAACACA  GATTTGACGTTTTCCCTCT CCAAT-5'
	                                                            TATTTGACGTTTTCCGACT
	                                                                  Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 001-231235899
Forward Primer: 5'-CTTGAGGCCAGTGCTTGTTTAGG-3' Tm: 59.148
Reverse Primer: 5'-TCCCCCTCTTGGCCAAAAGC-3' Tm: 59.508
Expected PCR Product Length: 326
Expected CelI Cleavage Product Sizes: 200, 126
In Silico PCR
Genome Browser

Rank: 25	Homology Score: 17
	Genomic Region: Intergenic	Closest Gene: LOC339622
Match Type: R15R
	          Right Nuclease
	         TCAGCCTTTTGCAGTTTAT
	5'-TAACC CCTGCCTTTTGGAGTTTAT        GCACAATTGACCAGC         ATTAACATTAAAAGACAGA TGATG-3'
	3'-ATTGG GGACGGAAAACCTCAAATA        CGTGTTAACTGGTCG         TAATTGTAATTTTCTGTCT ACTAC-5'
	                                                            TATTTGACGTTTTCCGACT
	                                                                  Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 021-026012528
Forward Primer: 5'-ACTCAGTGGATCCTCCTCTTGG-3' Tm: 59.258
Reverse Primer: 5'-CCACATGAGGGATGCCTTTGC-3' Tm: 59.378
Expected PCR Product Length: 326
Expected CelI Cleavage Product Sizes: 125, 201
In Silico PCR
Genome Browser

Rank: 26	Homology Score: 17
	Genomic Region: Intergenic	Closest Gene: CD300LG
Match Type: R16R
	          Right Nuclease
	         TCAGCCTTTTGCAGTTTAT
	5'-TACTA TCAGCCTTTTTCATTTTAA        CCATTCTAATAAATGT        ATAAACTGCACCAAGATTG TAGCT-3'
	3'-ATGAT AGTCGGAAAAAGTAAAATT        GGTAAGATTATTTACA        TATTTGACGTGGTTCTAAC ATCGA-5'
	                                                            TATTTGACGTTTTCCGACT
	                                                                  Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 017-041944294
Forward Primer: 5'-GGGCCATATGGTAGGTATATGCTC-3' Tm: 59.038
Reverse Primer: 5'-GACAAGTCACGCATTGGGAGAAG-3' Tm: 59.148
Expected PCR Product Length: 369
Expected CelI Cleavage Product Sizes: 134, 235
In Silico PCR
Genome Browser

Rank: 27	Homology Score: 17
	Genomic Region: Intergenic	Closest Gene: GXYLT1
Match Type: R24R
	          Right Nuclease
	         TCAGCCTTTTGCAGTTTAT
	5'-TACCC TCTCCCTTTTGCAGTTTAG    ACACAATTCACCAGCATTAGTAAA    ATAGAGATCATAAGACTGA CAGAA-3'
	3'-ATGGG AGAGGGAAAACGTCAAATC    TGTGTTAAGTGGTCGTAATCATTT    TATCTCTAGTATTCTGACT GTCTT-5'
	                                                            TATTTGACGTTTTCCGACT
	                                                                  Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 012-042468830
Forward Primer: 5'-GAATGGACACCTACCTTGGCC-3' Tm: 59.378
Reverse Primer: 5'-GTCTGGCAATGGATCAGCTGCA-3' Tm: 59.258
Expected PCR Product Length: 362
Expected CelI Cleavage Product Sizes: 130, 232
In Silico PCR
Genome Browser

Rank: 28	Homology Score: 17
	Genomic Region: Intergenic	Closest Gene: TMPRSS11B
Match Type: R30R
	          Right Nuclease
	         TCAGCCTTTTGCAGTTTAT
	5'-TTATG TCAGTCTTAAGATGTCTAT TTTAACATTAATGCTAGTCAGCTGTTATGC CTAAACTGCAAAAGGGAGA GAGTA-3'
	3'-AATAC AGTCAGAATTCTACAGATA AAATTGTAATTACGATCAGTCGACAATACG GATTTGACGTTTTCCCTCT CTCAT-5'
	                                                            TATTTGACGTTTTCCGACT
	                                                                  Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 004-069131937
Forward Primer: 5'-ATGGCCTGTGGGTGAGGCTT-3' Tm: 59.508
Reverse Primer: 5'-GGAGAATCACTTGAACCCGGG-3' Tm: 59.378
Expected PCR Product Length: 346
Expected CelI Cleavage Product Sizes: 127, 219
In Silico PCR
Genome Browser

Rank: 29	Homology Score: 17
	Genomic Region: Intergenic	Closest Gene: FAM75C2
Match Type: L20R
	           Left Nuclease
	         TTTGTGGGCAACATGCTGG
	5'-TATGA TTTTTGGGCAACATAGTGG      GACTCTGTCTACAAAACTAA      ATAAATAGAAAGAGGCCCA GTGCG-3'
	3'-ATACT AAAAACCCGTTGTATCACC      CTGAGACAGATGTTTTGATT      TATTTATCTTTCTCCGGGT CACGC-5'
	                                                            TATTTGACGTTTTCCGACT
	                                                                  Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 009-090818650
Forward Primer: 5'-CTGCAAGGACTCAAAAAATTTGACCCCTAC-3' Tm: 60.058
Reverse Primer: 5'-TCACTATATGGGCCAGGCTGATC-3' Tm: 59.148
Expected PCR Product Length: 350
Expected CelI Cleavage Product Sizes: 225, 125
In Silico PCR
Genome Browser

Rank: 30	Homology Score: 17
	Genomic Region: Intergenic	Closest Gene: FLJ42709
Match Type: R12L
	          Right Nuclease
	         TCAGCCTTTTGCAGTTTAT
	5'-ATATT CCAGCCTTTTCTTATTTCT          ATTCCATCCCTG          TGAACATGTTGCCCACAAA GTAGG-3'
	3'-TATAA GGTCGGAAAAGAATAAAGA          TAAGGTAGGGAC          ACTTGTACAACGGGTGTTT CATCC-5'
	                                                            GGTCGTACAACGGGTGTTT
	                                                                   Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 005-091821506
Forward Primer: 5'-AGGACTGGCTCCTTGTGGTG-3' Tm: 59.508
Reverse Primer: 5'-GACCTGCTTCTCTGGTCTTTTCTG-3' Tm: 59.038
Expected PCR Product Length: 563
Expected CelI Cleavage Product Sizes: 126, 437
In Silico PCR
Genome Browser

Rank: 31	Homology Score: 13
	Genomic Region: Intron	Closest Gene: MAP3K5
Match Type: R27R
	          Right Nuclease
	         TCAGCCTTTTGCAGTTTAT
	5'-ATACC TCCTCCCTTTGGAGTTTAG  GCACAACTGACCAGCATTACCATTAAA   ATAAAGATCATAAGGCTGA CAAAA-3'
	3'-TATGG AGGAGGGAAACCTCAAATC  CGTGTTGACTGGTCGTAATGGTAATTT   TATTTCTAGTATTCCGACT GTTTT-5'
	                                                            TATTTGACGTTTTCCGACT
	                                                                  Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 006-136890071
Forward Primer: 5'-GAAGAACCACCTACCCCCAG-3' Tm: 59.508
Reverse Primer: 5'-CTACAGAATGCAGATTTCCCCCAC-3' Tm: 59.038
Expected PCR Product Length: 348
Expected CelI Cleavage Product Sizes: 135, 213
In Silico PCR
Genome Browser

Rank: 32	Homology Score: 13
	Genomic Region: Intron	Closest Gene: KCNA6
Match Type: R24L
	          Right Nuclease
	         TCAGCCTTTTGCAGTTTAT
	5'-TTATC TGAGTCTTCTGAATTTTAT    AGCGGAAAGAAGGGATGCAGAAGG    CCAGCATCTTACCCAGGAA CGCTG-3'
	3'-AATAG ACTCAGAAGACTTAAAATA    TCGCCTTTCTTCCCTACGTCTTCC    GGTCGTAGAATGGGTCCTT GCGAC-5'
	                                                            GGTCGTACAACGGGTGTTT
	                                                                   Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 012-004929936
Forward Primer: 5'-GGGAGGACTAATGACTGAGGG-3' Tm: 59.378
Reverse Primer: 5'-CTCACCCTGGTCCTGTGATTTG-3' Tm: 59.258
Expected PCR Product Length: 397
Expected CelI Cleavage Product Sizes: 236, 161
In Silico PCR
Genome Browser

Rank: 33	Homology Score: 13
	Genomic Region: Intron	Closest Gene: RNU6-71
Match Type: R25L
	          Right Nuclease
	         TCAGCCTTTTGCAGTTTAT
	5'-CGCGG TAAGCAGTTTGCTGTTTAG   GCACTTCTTTTCGACCAATCATGTC    CCAGTTAGTTGCCCCCAAA ATCAA-3'
	3'-GCGCC ATTCGTCAAACGACAAATC   CGTGAAGAAAAGCTGGTTAGTACAG    GGTCAATCAACGGGGGTTT TAGTT-5'
	                                                            GGTCGTACAACGGGTGTTT
	                                                                   Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 006-056297715
Forward Primer: 5'-TCTACTCTCTTGGTGTCGCCATG-3' Tm: 59.148
Reverse Primer: 5'-AGTGGTGACTAGGACCGCGT-3' Tm: 59.508
Expected PCR Product Length: 364
Expected CelI Cleavage Product Sizes: 219, 145
In Silico PCR
Genome Browser

Rank: 34	Homology Score: 13
	Genomic Region: Intron	Closest Gene: ZFHX4
Match Type: R21R
	          Right Nuclease
	         TCAGCCTTTTGCAGTTTAT
	5'-TAATG TAAGATTTTTGCAGTATTT     TAAGATCTTTTGATAGACTAA      TTATATTGCAAAAGGTTGA TTGCA-3'
	3'-ATTAC ATTCTAAAAACGTCATAAA     ATTCTAGAAAACTATCTGATT      AATATAACGTTTTCCAACT AACGT-5'
	                                                            TATTTGACGTTTTCCGACT
	                                                                  Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 008-077661832
Forward Primer: 5'-GGGCCAGACATTTCTTTAGCCTAG-3' Tm: 59.038
Reverse Primer: 5'-GGAGACATCCTCAGGGAAAGC-3' Tm: 59.378
Expected PCR Product Length: 612
Expected CelI Cleavage Product Sizes: 265, 347
In Silico PCR
Genome Browser

Rank: 35	Homology Score: 13
	Genomic Region: Intergenic	Closest Gene: YTHDC2
Match Type: R27R
	          Right Nuclease
	         TCAGCCTTTTGCAGTTTAT
	5'-TTATG TCAGCCTTTTGAGCGCTAT  TTTAACCTTAATGCTGGTCAATTGTGC   ATAAACTCCAAAAGGGAAA GGGTA-3'
	3'-AATAC AGTCGGAAAACTCGCGATA  AAATTGGAATTACGACCAGTTAACACG   TATTTGAGGTTTTCCCTTT CCCAT-5'
	                                                            TATTTGACGTTTTCCGACT
	                                                                  Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 005-112991631
Forward Primer: 5'-TACTCCAGCCTGTGTGACAAGAGC-3' Tm: 60.908
Reverse Primer: 5'-CTACATGGATCTCCTCTTGGCC-3' Tm: 59.258
Expected PCR Product Length: 338
Expected CelI Cleavage Product Sizes: 206, 132
In Silico PCR
Genome Browser

Rank: 36	Homology Score: 13
	Genomic Region: Intergenic	Closest Gene: HS3ST5
Match Type: R18L
	          Right Nuclease
	         TCAGCCTTTTGCAGTTTAT
	5'-CCCCA TCTCCCTCTTGCAGGTTAT       ACCCTAAGCCTAGAGCAA       CCAGCATCGTGATTACAAA TAAGG-3'
	3'-GGGGT AGAGGGAGAACGTCCAATA       TGGGATTCGGATCTCGTT       GGTCGTAGCACTAATGTTT ATTCC-5'
	                                                            GGTCGTACAACGGGTGTTT
	                                                                   Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 006-114639685
Forward Primer: 5'-CCCACTAAGGCTCTTCACAGCA-3' Tm: 59.258
Reverse Primer: 5'-TAGGCATTGAGCACTTGCTAGGTC-3' Tm: 59.038
Expected PCR Product Length: 343
Expected CelI Cleavage Product Sizes: 134, 209
In Silico PCR
Genome Browser

Rank: 37	Homology Score: 13
	Genomic Region: Intergenic	Closest Gene: MAT2B
Match Type: L18R
	           Left Nuclease
	         TTTGTGGGCAACATGCTGG
	5'-GGGAG TTTTTGTGGAACATGATGG       GATGACATTAAAATAGTA       TGAAAATGCAAAATGCTAA TAATA-3'
	3'-CCCTC AAAAACACCTTGTACTACC       CTACTGTAATTTTATCAT       ACTTTTACGTTTTACGATT ATTAT-5'
	                                                            TATTTGACGTTTTCCGACT
	                                                                  Right Nuclease
Warning! Primer pair was generated using suboptimal parameters. 
Primer Index: 005-164243933
Forward Primer: 5'-CAGTAGACAATACGTAATACCTCCTGCAAG-3' Tm: 60.058
Reverse Primer: 5'-CTGCCCTACTCCATTTTAAAGATATTCTCCC-3' Tm: 59.958
Expected PCR Product Length: 526
Expected CelI Cleavage Product Sizes: 221, 305
In Silico PCR
Genome Browser

Rank: 38	Homology Score: 13
	Genomic Region: Intergenic	Closest Gene: KDM5D
Match Type: R25L
	          Right Nuclease
	         TCAGCCTTTTGCAGTTTAT
	5'-TATGG TAAGCACTTTGTTGTTTAT   GGGCTTCTTTTCCACCTATCAATTC    CCAGTTGGTTGCCCCCAAA ATGAA-3'
	3'-ATACC ATTCGTGAAACAACAAATA   CCCGAAGAAAAGGTGGATAGTTAAG    GGTCAACCAACGGGGGTTT TACTT-5'
	                                                            GGTCGTACAACGGGTGTTT
	                                                                   Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 00Y-022149362
Forward Primer: 5'-CATGACTGACACCCTCCACG-3' Tm: 59.508
Reverse Primer: 5'-AGCAAAGCAGGTGGCACAGC-3' Tm: 59.508
Expected PCR Product Length: 330
Expected CelI Cleavage Product Sizes: 203, 127
In Silico PCR
Genome Browser

Rank: 39	Homology Score: 13
	Genomic Region: Intergenic	Closest Gene: AOAH
Match Type: L24R
	           Left Nuclease
	         TTTGTGGGCAACATGCTGG
	5'-GTCCT CTTGTAGACAACATGCATG    CCTCCCTGCTCCCATTTGCTATCT    TTAATCTGCTAAAGGCTAA AAACC-3'
	3'-CAGGA GAACATCTGTTGTACGTAC    GGAGGGACGAGGGTAAACGATAGA    AATTAGACGATTTCCGATT TTTGG-5'
	                                                            TATTTGACGTTTTCCGACT
	                                                                  Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 007-036783522
Forward Primer: 5'-GGTTGTTTCTCTTCACTCACTGGC-3' Tm: 59.038
Reverse Primer: 5'-GCCCAACAATGCTGAACACAGATG-3' Tm: 59.038
Expected PCR Product Length: 352
Expected CelI Cleavage Product Sizes: 129, 223
In Silico PCR
Genome Browser

Rank: 40	Homology Score: 13
	Genomic Region: Intergenic	Closest Gene: OR7E156P
Match Type: R23R
	          Right Nuclease
	         TCAGCCTTTTGCAGTTTAT
	5'-CCTAC TGAGCCTTTTGCAGTACAA    ATCCCTGTGCTGTAAACAGTTGT     ATAAAATATAAAATTCTGA ACATT-3'
	3'-GGATG ACTCGGAAAACGTCATGTT    TAGGGACACGACATTTGTCAACA     TATTTTATATTTTAAGACT TGTAA-5'
	                                                            TATTTGACGTTTTCCGACT
	                                                                  Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 013-064120919
Forward Primer: 5'-CTCCTAGTCTACAAACCTCTGCAG-3' Tm: 59.038
Reverse Primer: 5'-CACCCTTAGCAAATAGGCAGAGAG-3' Tm: 59.038
Expected PCR Product Length: 461
Expected CelI Cleavage Product Sizes: 320, 141
In Silico PCR
Genome Browser

Rank: 41	Homology Score: 13
	Genomic Region: Intergenic	Closest Gene: ZP3
Match Type: L25R
	           Left Nuclease
	         TTTGTGGGCAACATGCTGG
	5'-CAGTC CTCATGGGCAACATGGGGG   TGGCACCTACTGAGCAGGATTAGGC    ATAAACTGATGAAAGCTGA AAGAT-3'
	3'-GTCAG GAGTACCCGTTGTACCCCC   ACCGTGGATGACTCGTCCTAATCCG    TATTTGACTACTTTCGACT TTCTA-5'
	                                                            TATTTGACGTTTTCCGACT
	                                                                  Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 007-076072549
Forward Primer: 5'-TTGCCCCAAACTCAAGCCATTCTC-3' Tm: 59.038
Reverse Primer: 5'-CCAACGTTCACAGACCACACTTAG-3' Tm: 59.038
Expected PCR Product Length: 340
Expected CelI Cleavage Product Sizes: 125, 215
In Silico PCR
Genome Browser

Rank: 42	Homology Score: 13
	Genomic Region: Intergenic	Closest Gene: MIR4300
Match Type: R28R
	          Right Nuclease
	         TCAGCCTTTTGCAGTTTAT
	5'-TTTGG TCTTCCTTTTCCACTTTAT  CTTTTATACTGCTATTGCTATGGTCATT  CTAAACTGGAAATAGCAGA AGTAG-3'
	3'-AAACC AGAAGGAAAAGGTGAAATA  GAAAATATGACGATAACGATACCAGTAA  GATTTGACCTTTATCGTCT TCATC-5'
	                                                            TATTTGACGTTTTCCGACT
	                                                                  Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 011-080866146
Forward Primer: 5'-CCCAGAAACCACCAACTAGCATG-3' Tm: 59.148
Reverse Primer: 5'-CTGGAGATCTGAGACCATGCG-3' Tm: 59.378
Expected PCR Product Length: 568
Expected CelI Cleavage Product Sizes: 396, 172
In Silico PCR
Genome Browser

Rank: 43	Homology Score: 13
	Genomic Region: Intergenic	Closest Gene: LOC728084
Match Type: L17R
	           Left Nuclease
	         TTTGTGGGCAACATGCTGG
	5'-CTTTA TCTGAGGGCAACATCCTAC       CACAATTCCCATAATAA        ATAGACTGCACAAGAGTGA GAAGC-3'
	3'-GAAAT AGACTCCCGTTGTAGGATG       GTGTTAAGGGTATTATT        TATCTGACGTGTTCTCACT CTTCG-5'
	                                                            TATTTGACGTTTTCCGACT
	                                                                  Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 012-089421427
Forward Primer: 5'-GCATTCACTCGCCTGTTCCTC-3' Tm: 59.378
Reverse Primer: 5'-GCTGACTTAAGAGGGCTCTTTGC-3' Tm: 59.148
Expected PCR Product Length: 332
Expected CelI Cleavage Product Sizes: 125, 207
In Silico PCR
Genome Browser

Rank: 44	Homology Score: 10
	Genomic Region: Exon	Closest Gene: DAPP1
Match Type: R20R
	          Right Nuclease
	         TCAGCCTTTTGCAGTTTAT
	5'-TATTT TCACCCTGTTGGGGTAAAT      GTTTTAAAGAGTGAGAAAAC      ATAAATTGAGAAAGGGTGA TAAAG-3'
	3'-ATAAA AGTGGGACAACCCCATTTA      CAAAATTTCTCACTCTTTTG      TATTTAACTCTTTCCCACT ATTTC-5'
	                                                            TATTTGACGTTTTCCGACT
	                                                                  Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 004-100789762
Forward Primer: 5'-GAGGCTTAGATGACTTCTGCAGG-3' Tm: 59.148
Reverse Primer: 5'-CTCTTGGGCCAACCCTATACAAC-3' Tm: 59.148
Expected PCR Product Length: 530
Expected CelI Cleavage Product Sizes: 316, 214
In Silico PCR
Genome Browser

Rank: 45	Homology Score: 10
	Genomic Region: Exon	Closest Gene: PLP1
Match Type: R14R
	          Right Nuclease
	         TCAGCCTTTTGCAGTTTAT
	5'-AGATT TCAGTCTTTTCCTGTTTTT         GTTAACTTTGGGTT         AAAAAAAAAAAAAGTCTGA TTGGT-3'
	3'-TCTAA AGTCAGAAAAGGACAAAAA         CAATTGAAACCCAA         TTTTTTTTTTTTTCAGACT AACCA-5'
	                                                            TATTTGACGTTTTCCGACT
	                                                                  Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 00X-103046628
Forward Primer: 5'-GGGTTGCATCAGAAATGTCTGGAG-3' Tm: 59.038
Reverse Primer: 5'-CCTTCTATGCAGCCTGGAAGC-3' Tm: 59.378
Expected PCR Product Length: 620
Expected CelI Cleavage Product Sizes: 416, 204
In Silico PCR
Genome Browser

Rank: 46	Homology Score: 10
	Genomic Region: Intron	Closest Gene: CHUK
Match Type: R10R
	          Right Nuclease
	         TCAGCCTTTTGCAGTTTAT
	5'-AGGAG TTAGCCATATGTAGTTTAT           AAGTAAAGCT           ATACAGTTGAAGAGGCTGC CTAAT-3'
	3'-TCCTC AATCGGTATACATCAAATA           TTCATTTCGA           TATGTCAACTTCTCCGACG GATTA-5'
	                                                            TATTTGACGTTTTCCGACT
	                                                                  Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 010-101975046
Forward Primer: 5'-TGTAAGTGCCAGTGGAACAGCC-3' Tm: 59.258
Reverse Primer: 5'-GCAGCAGAGCATCATGAGCAAAAG-3' Tm: 59.038
Expected PCR Product Length: 563
Expected CelI Cleavage Product Sizes: 126, 437
In Silico PCR
Genome Browser

Rank: 47	Homology Score: 10
	Genomic Region: Intron	Closest Gene: NAPEPLD
Match Type: R17R
	          Right Nuclease
	         TCAGCCTTTTGCAGTTTAT
	5'-CTAAC TCATAGTTATGAGGTTTAT       TCAACATCAAAACATGA        ACAAATTCCAAAATGCTGA AAGTC-3'
	3'-GATTG AGTATCAATACTCCAAATA       AGTTGTAGTTTTGTACT        TGTTTAAGGTTTTACGACT TTCAG-5'
	                                                            TATTTGACGTTTTCCGACT
	                                                                  Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 007-102753028
Forward Primer: 5'-CATCACACATTGGGGCCTGTC-3' Tm: 59.378
Reverse Primer: 5'-GAGATGGGGAAGATGAGAGAGG-3' Tm: 59.258
Expected PCR Product Length: 440
Expected CelI Cleavage Product Sizes: 270, 170
In Silico PCR
Genome Browser

Rank: 48	Homology Score: 10
	Genomic Region: Intron	Closest Gene: PDGFD
Match Type: R21R
	          Right Nuclease
	         TCAGCCTTTTGCAGTTTAT
	5'-AAACG AGAAGCTTTTGCAGTGGAT     TTGGACCCAGGGAACTTCCCC      AGATACTCCAAAAGGATGA GACAA-3'
	3'-TTTGC TCTTCGAAAACGTCACCTA     AACCTGGGTCCCTTGAAGGGG      TCTATGAGGTTTTCCTACT CTGTT-5'
	                                                            TATTTGACGTTTTCCGACT
	                                                                  Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 011-103816969
Forward Primer: 5'-GCTCCTGCTCCAGAAAGTTCTTG-3' Tm: 59.148
Reverse Primer: 5'-CAGACACCAGCAGTTTTTGCAACC-3' Tm: 59.038
Expected PCR Product Length: 343
Expected CelI Cleavage Product Sizes: 132, 211
In Silico PCR
Genome Browser

Plate format Oligo Order Form
Well
Name
Sequence
5' mods
Internal
Notes
Primer Plate #1
A1
003-046414377F
GCACAGGGTGGAACAAGATGG
12212012SXJ93rlv_QUEUE_Ranking-Homology	Primer pair generated using optimal parameters. 
A2
003-046414377R
GTGACCGTCCTGGCTTTTAAAGCA
12212012SXJ93rlv_QUEUE_Ranking-Homology	Primer pair generated using optimal parameters. 
B1
003-046399009F
CATCCACAACATGCTGTCCACATC
12212012SXJ93rlv_QUEUE_Ranking-Homology	Primer pair generated using optimal parameters. 
B2
003-046399009R
CAAAGACCCACTCATTTGCAGCAG
12212012SXJ93rlv_QUEUE_Ranking-Homology	Primer pair generated using optimal parameters. 
C1
010-127381042F
CATCTCACGCAAAAAGACACCACC
12212012SXJ93rlv_QUEUE_Ranking-Homology	Primer pair generated using optimal parameters. 
C2
010-127381042R
GGACTCACAAGGACAGGCTC
12212012SXJ93rlv_QUEUE_Ranking-Homology	Primer pair generated using optimal parameters. 
D1
012-032991814F
GCTTTTGCTGGGGAAGCTGG
12212012SXJ93rlv_QUEUE_Ranking-Homology	Primer pair generated using optimal parameters. 
D2
012-032991814R
CAAGCCTGGATCAGATACCTCCT
12212012SXJ93rlv_QUEUE_Ranking-Homology	Primer pair generated using optimal parameters. 
E1
010-007305349F
CGTGCCACTAATGCCCAGGA
12212012SXJ93rlv_QUEUE_Ranking-Homology	Primer pair generated using optimal parameters. 
E2
010-007305349R
TTTGGCCTTCAGGTGGGACACT
12212012SXJ93rlv_QUEUE_Ranking-Homology	Primer pair generated using optimal parameters. 
F1
003-063153302F
TCTGGGCTGGGCTGTGCTTT
12212012SXJ93rlv_QUEUE_Ranking-Homology	Primer pair generated using optimal parameters. 
F2
003-063153302R
CCTAGTTGCTCCTTAGTACTTCCC
12212012SXJ93rlv_QUEUE_Ranking-Homology	Primer pair generated using optimal parameters. 
G1
004-008527974F
GCTCAAGTGTGAGGGTGCTG
12212012SXJ93rlv_QUEUE_Ranking-Homology	Primer pair generated using optimal parameters. 
G2
004-008527974R
TAAGGAGGCTGGAGGGAGCA
12212012SXJ93rlv_QUEUE_Ranking-Homology	Primer pair generated using optimal parameters. 
H1
002-116474320F
CATTTGTGGCCAAAGGTGCCTTGA
12212012SXJ93rlv_QUEUE_Ranking-Homology	Primer pair generated using optimal parameters. 
H2
002-116474320R
CCCTCCCTCTCATTCATCCAAAG
12212012SXJ93rlv_QUEUE_Ranking-Homology	Primer pair generated using optimal parameters. 
A3
007-076237892F
ACATGTCTGTACCCGAGTCAACAC
12212012SXJ93rlv_QUEUE_Ranking-Homology	Primer pair generated using optimal parameters. 
A4
007-076237892R
ACTTGTGCCCCCTTTGCTGG
12212012SXJ93rlv_QUEUE_Ranking-Homology	Primer pair generated using optimal parameters. 
B3
001-112045670F
CCAGCAAGGACATGATGGAGG
12212012SXJ93rlv_QUEUE_Ranking-Homology	Primer pair generated using optimal parameters. 
B4
001-112045670R
TCCCCTGGGAAGGCAAGATG
12212012SXJ93rlv_QUEUE_Ranking-Homology	Primer pair generated using optimal parameters. 
C3
002-160206641F
CAGGAGGCATCTTAGCTACTTCCA
12212012SXJ93rlv_QUEUE_Ranking-Homology	Primer pair generated using optimal parameters. 
C4
002-160206641R
AATTCACGCCATTGCACTCCAGC
12212012SXJ93rlv_QUEUE_Ranking-Homology	Primer pair generated using optimal parameters. 
D3
003-157066450F
AAGGAAGAGGGGGCTGCAGA
12212012SXJ93rlv_QUEUE_Ranking-Homology	Primer pair generated using optimal parameters. 
D4
003-157066450R
GCTGAGTCCTTGTCTGGTTTTGG
12212012SXJ93rlv_QUEUE_Ranking-Homology	Primer pair generated using optimal parameters. 
E3
018-020747199F
CTTTCCAGGCTGCTTTGGGAG
12212012SXJ93rlv_QUEUE_Ranking-Homology	Primer pair generated using optimal parameters. 
E4
018-020747199R
CTCCCCCCAATCCCCCAAAA
12212012SXJ93rlv_QUEUE_Ranking-Homology	Primer pair generated using optimal parameters. 
F3
004-020877272F
GCCTAGGTCAGAGCCTTTAGC
12212012SXJ93rlv_QUEUE_Ranking-Homology	Primer pair generated using optimal parameters. 
F4
004-020877272R
GCACTATCTCAGTGCCCTCATTC
12212012SXJ93rlv_QUEUE_Ranking-Homology	Primer pair generated using optimal parameters. 
G3
022-021114662F
GTGCCCTTACACTAAAACTGGCC
12212012SXJ93rlv_QUEUE_Ranking-Homology	Primer pair generated using optimal parameters. 
G4
022-021114662R
CTGGGAAGACAGAGAAATGGTGG
12212012SXJ93rlv_QUEUE_Ranking-Homology	Primer pair generated using optimal parameters. 
H3
020-044711346F
GTGTCCAAGTGGTGGTCTGAAAAC
12212012SXJ93rlv_QUEUE_Ranking-Homology	Primer pair generated using optimal parameters. 
H4
020-044711346R
CCGCTCTTGTTATTCCTAGTCCTC
12212012SXJ93rlv_QUEUE_Ranking-Homology	Primer pair generated using optimal parameters. 
A5
011-076680948F
CCTGCATATTGTGCACATGTACCC
12212012SXJ93rlv_QUEUE_Ranking-Homology	Primer pair generated using optimal parameters. 
A6
011-076680948R
GGATGAACCCCTCTTGGCCA
12212012SXJ93rlv_QUEUE_Ranking-Homology	Primer pair generated using optimal parameters. 
B5
003-089353684F
GTTCACTAGGCCAGGAACAGTC
12212012SXJ93rlv_QUEUE_Ranking-Homology	Primer pair generated using optimal parameters. 
B6
003-089353684R
GCATGGCTAAAGAAGGCCAACATC
12212012SXJ93rlv_QUEUE_Ranking-Homology	Primer pair generated using optimal parameters. 
C5
002-105729897F
TGAGTGGACTCCCTCTTGGC
12212012SXJ93rlv_QUEUE_Ranking-Homology	Primer pair generated using optimal parameters. 
C6
002-105729897R
GGTGGATGAGCTGCAACTAGTTC
12212012SXJ93rlv_QUEUE_Ranking-Homology	Primer pair generated using optimal parameters. 
D5
004-113138626F
GCCATCCAAATGTGGACAGTAGG
12212012SXJ93rlv_QUEUE_Ranking-Homology	Primer pair generated using optimal parameters. 
D6
004-113138626R
GTAGGGGAAACCAATTACAGTGCC
12212012SXJ93rlv_QUEUE_Ranking-Homology	Primer pair generated using optimal parameters. 
E5
019-012111242F
GTAGTGTCGTCTCATGGGCAAAAG
12212012SXJ93rlv_QUEUE_Ranking-Homology	Primer pair generated using optimal parameters. 
E6
019-012111242R
CAGTCCTGAGAGTGTGTTTCCTTG
12212012SXJ93rlv_QUEUE_Ranking-Homology	Primer pair generated using optimal parameters. 
F5
003-015921847F
GCTCTAGAACTCTAGACTGTGGG
12212012SXJ93rlv_QUEUE_Ranking-Homology	Primer pair generated using optimal parameters. 
F6
003-015921847R
CTCACGGCCAATCCTGTTTTATCC
12212012SXJ93rlv_QUEUE_Ranking-Homology	Primer pair generated using optimal parameters. 
G5
003-177863333F
CAGTCTGGAGACCTCCAGTAG
12212012SXJ93rlv_QUEUE_Ranking-Homology	Primer pair generated using optimal parameters. 
G6
003-177863333R
GTCCTTCCAAGGCATCACCATTTC
12212012SXJ93rlv_QUEUE_Ranking-Homology	Primer pair generated using optimal parameters. 
H5
001-231235899F
CTTGAGGCCAGTGCTTGTTTAGG
12212012SXJ93rlv_QUEUE_Ranking-Homology	Primer pair generated using optimal parameters. 
H6
001-231235899R
TCCCCCTCTTGGCCAAAAGC
12212012SXJ93rlv_QUEUE_Ranking-Homology	Primer pair generated using optimal parameters. 
A7
021-026012528F
ACTCAGTGGATCCTCCTCTTGG
12212012SXJ93rlv_QUEUE_Ranking-Homology	Primer pair generated using optimal parameters. 
A8
021-026012528R
CCACATGAGGGATGCCTTTGC
12212012SXJ93rlv_QUEUE_Ranking-Homology	Primer pair generated using optimal parameters. 
B7
017-041944294F
GGGCCATATGGTAGGTATATGCTC
12212012SXJ93rlv_QUEUE_Ranking-Homology	Primer pair generated using optimal parameters. 
B8
017-041944294R
GACAAGTCACGCATTGGGAGAAG
12212012SXJ93rlv_QUEUE_Ranking-Homology	Primer pair generated using optimal parameters. 
C7
012-042468830F
GAATGGACACCTACCTTGGCC
12212012SXJ93rlv_QUEUE_Ranking-Homology	Primer pair generated using optimal parameters. 
C8
012-042468830R
GTCTGGCAATGGATCAGCTGCA
12212012SXJ93rlv_QUEUE_Ranking-Homology	Primer pair generated using optimal parameters. 
D7
004-069131937F
ATGGCCTGTGGGTGAGGCTT
12212012SXJ93rlv_QUEUE_Ranking-Homology	Primer pair generated using optimal parameters. 
D8
004-069131937R
GGAGAATCACTTGAACCCGGG
12212012SXJ93rlv_QUEUE_Ranking-Homology	Primer pair generated using optimal parameters. 
E7
009-090818650F
CTGCAAGGACTCAAAAAATTTGACCCCTAC
12212012SXJ93rlv_QUEUE_Ranking-Homology	Primer pair generated using optimal parameters. 
E8
009-090818650R
TCACTATATGGGCCAGGCTGATC
12212012SXJ93rlv_QUEUE_Ranking-Homology	Primer pair generated using optimal parameters. 
F7
005-091821506F
AGGACTGGCTCCTTGTGGTG
12212012SXJ93rlv_QUEUE_Ranking-Homology	Primer pair generated using optimal parameters. 
F8
005-091821506R
GACCTGCTTCTCTGGTCTTTTCTG
12212012SXJ93rlv_QUEUE_Ranking-Homology	Primer pair generated using optimal parameters. 
G7
006-136890071F
GAAGAACCACCTACCCCCAG
12212012SXJ93rlv_QUEUE_Ranking-Homology	Primer pair generated using optimal parameters. 
G8
006-136890071R
CTACAGAATGCAGATTTCCCCCAC
12212012SXJ93rlv_QUEUE_Ranking-Homology	Primer pair generated using optimal parameters. 
H7
012-004929936F
GGGAGGACTAATGACTGAGGG
12212012SXJ93rlv_QUEUE_Ranking-Homology	Primer pair generated using optimal parameters. 
H8
012-004929936R
CTCACCCTGGTCCTGTGATTTG
12212012SXJ93rlv_QUEUE_Ranking-Homology	Primer pair generated using optimal parameters. 
A9
006-056297715F
TCTACTCTCTTGGTGTCGCCATG
12212012SXJ93rlv_QUEUE_Ranking-Homology	Primer pair generated using optimal parameters. 
A10
006-056297715R
AGTGGTGACTAGGACCGCGT
12212012SXJ93rlv_QUEUE_Ranking-Homology	Primer pair generated using optimal parameters. 
B9
008-077661832F
GGGCCAGACATTTCTTTAGCCTAG
12212012SXJ93rlv_QUEUE_Ranking-Homology	Primer pair generated using optimal parameters. 
B10
008-077661832R
GGAGACATCCTCAGGGAAAGC
12212012SXJ93rlv_QUEUE_Ranking-Homology	Primer pair generated using optimal parameters. 
C9
005-112991631F
TACTCCAGCCTGTGTGACAAGAGC
12212012SXJ93rlv_QUEUE_Ranking-Homology	Primer pair generated using optimal parameters. 
C10
005-112991631R
CTACATGGATCTCCTCTTGGCC
12212012SXJ93rlv_QUEUE_Ranking-Homology	Primer pair generated using optimal parameters. 
D9
006-114639685F
CCCACTAAGGCTCTTCACAGCA
12212012SXJ93rlv_QUEUE_Ranking-Homology	Primer pair generated using optimal parameters. 
D10
006-114639685R
TAGGCATTGAGCACTTGCTAGGTC
12212012SXJ93rlv_QUEUE_Ranking-Homology	Primer pair generated using optimal parameters. 
E9
005-164243933F
CAGTAGACAATACGTAATACCTCCTGCAAG
12212012SXJ93rlv_QUEUE_Ranking-Homology	Warning! Primer pair was generated using suboptimal parameters. 
E10
005-164243933R
CTGCCCTACTCCATTTTAAAGATATTCTCCC
12212012SXJ93rlv_QUEUE_Ranking-Homology	Warning! Primer pair was generated using suboptimal parameters. 
F9
00Y-022149362F
CATGACTGACACCCTCCACG
12212012SXJ93rlv_QUEUE_Ranking-Homology	Primer pair generated using optimal parameters. 
F10
00Y-022149362R
AGCAAAGCAGGTGGCACAGC
12212012SXJ93rlv_QUEUE_Ranking-Homology	Primer pair generated using optimal parameters. 
G9
007-036783522F
GGTTGTTTCTCTTCACTCACTGGC
12212012SXJ93rlv_QUEUE_Ranking-Homology	Primer pair generated using optimal parameters. 
G10
007-036783522R
GCCCAACAATGCTGAACACAGATG
12212012SXJ93rlv_QUEUE_Ranking-Homology	Primer pair generated using optimal parameters. 
H9
013-064120919F
CTCCTAGTCTACAAACCTCTGCAG
12212012SXJ93rlv_QUEUE_Ranking-Homology	Primer pair generated using optimal parameters. 
H10
013-064120919R
CACCCTTAGCAAATAGGCAGAGAG
12212012SXJ93rlv_QUEUE_Ranking-Homology	Primer pair generated using optimal parameters. 
A11
007-076072549F
TTGCCCCAAACTCAAGCCATTCTC
12212012SXJ93rlv_QUEUE_Ranking-Homology	Primer pair generated using optimal parameters. 
A12
007-076072549R
CCAACGTTCACAGACCACACTTAG
12212012SXJ93rlv_QUEUE_Ranking-Homology	Primer pair generated using optimal parameters. 
B11
011-080866146F
CCCAGAAACCACCAACTAGCATG
12212012SXJ93rlv_QUEUE_Ranking-Homology	Primer pair generated using optimal parameters. 
B12
011-080866146R
CTGGAGATCTGAGACCATGCG
12212012SXJ93rlv_QUEUE_Ranking-Homology	Primer pair generated using optimal parameters. 
C11
012-089421427F
GCATTCACTCGCCTGTTCCTC
12212012SXJ93rlv_QUEUE_Ranking-Homology	Primer pair generated using optimal parameters. 
C12
012-089421427R
GCTGACTTAAGAGGGCTCTTTGC
12212012SXJ93rlv_QUEUE_Ranking-Homology	Primer pair generated using optimal parameters. 
D11
004-100789762F
GAGGCTTAGATGACTTCTGCAGG
12212012SXJ93rlv_QUEUE_Ranking-Homology	Primer pair generated using optimal parameters. 
D12
004-100789762R
CTCTTGGGCCAACCCTATACAAC
12212012SXJ93rlv_QUEUE_Ranking-Homology	Primer pair generated using optimal parameters. 
E11
00X-103046628F
GGGTTGCATCAGAAATGTCTGGAG
12212012SXJ93rlv_QUEUE_Ranking-Homology	Primer pair generated using optimal parameters. 
E12
00X-103046628R
CCTTCTATGCAGCCTGGAAGC
12212012SXJ93rlv_QUEUE_Ranking-Homology	Primer pair generated using optimal parameters. 
F11
010-101975046F
TGTAAGTGCCAGTGGAACAGCC
12212012SXJ93rlv_QUEUE_Ranking-Homology	Primer pair generated using optimal parameters. 
F12
010-101975046R
GCAGCAGAGCATCATGAGCAAAAG
12212012SXJ93rlv_QUEUE_Ranking-Homology	Primer pair generated using optimal parameters. 
G11
007-102753028F
CATCACACATTGGGGCCTGTC
12212012SXJ93rlv_QUEUE_Ranking-Homology	Primer pair generated using optimal parameters. 
G12
007-102753028R
GAGATGGGGAAGATGAGAGAGG
12212012SXJ93rlv_QUEUE_Ranking-Homology	Primer pair generated using optimal parameters. 
H11
011-103816969F
GCTCCTGCTCCAGAAAGTTCTTG
12212012SXJ93rlv_QUEUE_Ranking-Homology	Primer pair generated using optimal parameters. 
H12
011-103816969R
CAGACACCAGCAGTTTTTGCAACC
12212012SXJ93rlv_QUEUE_Ranking-Homology	Primer pair generated using optimal parameters.