PROGNOS Summary for Tesson_IgM_Ranking-TALENv2.0

The rn4 genome was searched for the TALEN pair targeting:
5'- TTCCTGCCCAGCTCCAT NN..NN ACCAGAACAACACTGAA -3'

Using these RVDs:
Left:  01NG02HD03HD04NG05NN06HD07HD08HD09NI10NN11HD12NG13HD14HD15NI16NG
Right: 01NG02HD03NI04NN05NG06NN07NG08NG09NN10NG11NG12HD13NG14NN15NN16NG

Up to 5 mismatches were allowed in each nuclease half-site.

Allowed spacing distances were: 12 13 14 15 16 17 18 19 20 21 22 23 24

Only heterodimers of the nuclease pair were included in the output

2541 total sites were located
Mismatch Types
Genomic Regions
  0 and 0: 1
  3 and 3: 1
  2 and 5: 3
  3 and 4: 12
  3 and 5: 61
  4 and 4: 23
  4 and 5: 538
  5 and 5: 1902
  Exons: 47
  Promoters: 13
  Introns: 646
  Intergenic: 1835


The top 100 sites are ranked below according to parameters optimized for TALEN off-target prediction.
For the full output, click here.

This output will be deleted from the server one week after it is generated.  Please save your results.

Primer pairs were designed using the 400 bp of sequence on either side of the cleavage site to melt close to 60 °C

For easy visualization of cleavage on gels:
The smallest fragment will be longer than 130 bp, which is also the minimum separation distance between uncleaved and cleaved products.
The two cleavage products will be separated by a minimum size of 0 bp
To optimize assay conditions, the amplicons have a minimum length of 260 bp, a maximum length of 450 bp, and an optimal length of 270 bp.
Rank: 1	Homology Score: 72	TALEN Score: 100
	Genomic Region: Intergenic	Closest Gene: Adam6	Caution! This site scores very closely to the intended nuclease target. The potential for cleavage at this site is of great concern.
Match Type: R17L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-ATGAC TTCAGTGTTGTTCTGGT    AGTTCCAGGAGAAGGAA     ATGGAGCTGGGCAGGAA GTCCC-3'
	3'-TACTG AAGTCACAACAAGACCA    TCAAGGTCCTCTTCCTT     TACCTCGACCCGTCCTT CAGGG-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 006-138443970
Forward Primer: 5'-GGAATCGGCACATGCAGATCTTTG-3' Tm: 59.038
Reverse Primer: 5'-TCTGGTCCTCAGAGAGTCAGTC-3' Tm: 59.258
Expected PCR Product Length: 325
Expected CelI Cleavage Product Sizes: 194, 131
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr6:138444137-138444187

Rank: 2	Homology Score: 17	TALEN Score: 62.39
	Genomic Region: Intergenic	Closest Gene: RGD1559903
Match Type: R16L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-TTCCA TGCAGTGTTGTTCTGTT     GTCAGTAATGGATGCC     AGAGAGTTGGGATGGAA TGAGA-3'
	3'-AAGGT ACGTCACAACAAGACAA     CAGTCATTACCTACGG     TCTCTCAACCCTACCTT ACTCT-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 020-016460972
Forward Primer: 5'-GTCAAGGACGAAGTCATCTGGAG-3' Tm: 59.148
Reverse Primer: 5'-GACAAGCTGACTGCTGCTAGAG-3' Tm: 59.258
Expected PCR Product Length: 340
Expected CelI Cleavage Product Sizes: 144, 196
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr20:16461089-16461138

Rank: 3	Homology Score: 18	TALEN Score: 61.93
	Genomic Region: Intron	Closest Gene: Pdcd11
Match Type: R16L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-TTGGA TGCAGACTTGTTCTGGT     CCTGTTAGCAGACCTC     AGGGATCTGGGGAGGAA TTAGC-3'
	3'-AACCT ACGTCTGAACAAGACCA     GGACAATCGTCTGGAG     TCCCTAGACCCCTCCTT AATCG-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 001-252428669
Forward Primer: 5'-GTTGTGTTTCAGGACAGCACTACC-3' Tm: 59.038
Reverse Primer: 5'-GTTGGAGAGGCCACATTCTTAACC-3' Tm: 59.038
Expected PCR Product Length: 308
Expected CelI Cleavage Product Sizes: 162, 146
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr1:252428804-252428853

Rank: 4	Homology Score: 17	TALEN Score: 61.83
	Genomic Region: Intergenic	Closest Gene: Lrfn2
Match Type: L24R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-TCCCC TTCCTGCCCAGCTCCCA TCCCCACCCCCTTCTGAGAACTAA ACAGGAAGCACACGGAA CCTGC-3'
	3'-AGGGG AAGGACGGGTCGAGGGT AGGGGTGGGGGAAGACTCTTGATT TGTCCTTCGTGTGCCTT GGACG-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 009-007190270
Forward Primer: 5'-AGTTGGGCTGGTAGAAATTGCTGG-3' Tm: 59.038
Reverse Primer: 5'-CTGAGTGAAACAAACGGCTCCGA-3' Tm: 59.148
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 133, 137
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr9:7190372-7190429

Rank: 5	Homology Score: 13	TALEN Score: 60.64
	Genomic Region: Intergenic	Closest Gene: Tssc1
Match Type: L17R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-GGGGG CTCCTGCCCAGATCCTT    CTCTGCCAGGCTGCCCC     TCCAGCACAATATTGAA TCTCA-3'
	3'-CCCCC GAGGACGGGTCTAGGAA    GAGACGGTCCGACGGGG     AGGTCGTGTTATAACTT AGAGT-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 006-046986724
Forward Primer: 5'-CTTCTGGCTTAGTTTCCGTCTGTC-3' Tm: 59.038
Reverse Primer: 5'-CTCAGCTTGCCCAAGGGTTATAG-3' Tm: 59.148
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 130, 140
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr6:46986827-46986877

Rank: 6	Homology Score: 13	TALEN Score: 59.43
	Genomic Region: Intron	Closest Gene: Acvrl1
Match Type: R21L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-AAGGC TTCATTGAGGTTCTGGT  CTTCATGATCACAGAAGCGGC   CTGGAGTTGGGAAGGAG GAAGA-3'
	3'-TTCCG AAGTAACTCCAAGACCA  GAAGTACTAGTGTCTTCGCCG   GACCTCAACCCTTCCTC CTTCT-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 007-139879252
Forward Primer: 5'-AAAGGCTCATTAGACACGCTCAGG-3' Tm: 59.038
Reverse Primer: 5'-CACGTCTCTGCAGACCCCAT-3' Tm: 59.508
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 134, 136
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr7:139879357-139879411

Rank: 7	Homology Score: 8	TALEN Score: 59.39
	Genomic Region: Intergenic	Closest Gene: Prdm2
Match Type: R19L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-TACCT TTCAGGGTTGTTCTAAA   AACTAACTGGAGAACACAA    ATGGCGCCTGGCATGAA GTAAC-3'
	3'-ATGGA AAGTCCCAACAAGATTT   TTGATTGACCTCTTGTGTT    TACCGCGGACCGTACTT CATTG-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 005-161756246
Forward Primer: 5'-CTCTAGGGGCTAAGTATCTCAGCT-3' Tm: 59.038
Reverse Primer: 5'-GGTGGCAAAATCTAAACCCTGTGG-3' Tm: 59.038
Expected PCR Product Length: 280
Expected CelI Cleavage Product Sizes: 130, 150
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr5:161756348-161756400

Rank: 8	Homology Score: 10	TALEN Score: 59.07
	Genomic Region: Intergenic	Closest Gene: Mir206
Match Type: L23R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-TTTTA TTAGTGCCCAAATCTAT AGTAGTAATGGTCTAATTTGAGC  TCCAGAATAACTCTGAA TCAAA-3'
	3'-AAAAT AATCACGGGTTTAGATA TCATCATTACCAGATTAAACTCG  AGGTCTTATTGAGACTT AGTTT-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 009-019047855
Forward Primer: 5'-GTGGCTTTGGTCCTCATCTGTC-3' Tm: 59.258
Reverse Primer: 5'-CCTCTTTCGGAGATAGGCATGTTC-3' Tm: 59.038
Expected PCR Product Length: 302
Expected CelI Cleavage Product Sizes: 161, 141
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr9:19047986-19048042

Rank: 9	Homology Score: 13	TALEN Score: 58.86
	Genomic Region: Intron	Closest Gene: Sars
Match Type: L22R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-GTAAG TACCTCCCCAGCTCAAT  TCCACTCACACAACCACAGATA  ACCAGGCCAAAACAGAA GTACC-3'
	3'-CATTC ATGGAGGGGTCGAGTTA  AGGTGAGTGTGTTGGTGTCTAT  TGGTCCGGTTTTGTCTT CATGG-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 002-204001007
Forward Primer: 5'-AATTTATGGGCTCTGTGCCGTGC-3' Tm: 59.148
Reverse Primer: 5'-CCCAGTGCTAAGTTGAGTGTGGC-3' Tm: 61.088
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 135, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr2:204001112-204001167

Rank: 10	Homology Score: 10	TALEN Score: 58.68
	Genomic Region: Intergenic	Closest Gene: Pard3
Match Type: R12L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-TTTGC TTTTTTATTGTTCTGGA       AGTTAAGTTTGA       ATGGGGCTTGGTAGGAA ATGAT-3'
	3'-AAACG AAAAAATAACAAGACCT       TCAATTCAAACT       TACCCCGAACCATCCTT TACTA-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 019-057896612
Forward Primer: 5'-CCCAGTGCTGGGGTTACAAACA-3' Tm: 59.258
Reverse Primer: 5'-TCTTAGCCAGGAATGAGGCTGC-3' Tm: 59.258
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 138, 132
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr19:57896725-57896770

Rank: 11	Homology Score: 13	TALEN Score: 58.5
	Genomic Region: Intergenic	Closest Gene: Wdfy3
Match Type: L23R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-TTTCT TTCAGTCCCAGCTCCAT TTTTGTCCCTGCATTGCCTTTAG  ACAGGAACAACTCTGAG TTGCA-3'
	3'-AAAGA AAGTCAGGGTCGAGGTA AAAACAGGGACGTAACGGAAATC  TGTCCTTGTTGAGACTC AACGT-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 014-008802083
Forward Primer: 5'-TGCCAGGCTCCTGTCTGCAT-3' Tm: 59.508
Reverse Primer: 5'-GCTACCAGGACTCAATGGGG-3' Tm: 59.508
Expected PCR Product Length: 299
Expected CelI Cleavage Product Sizes: 139, 160
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr14:8802192-8802248

Rank: 12	Homology Score: 17	TALEN Score: 58.39
	Genomic Region: Exon	Closest Gene: Mapk6
Match Type: R15L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-ATACA TTCAGTGTTGGACTGGT     CCTGAGCAGCATCCT      AAGGCGCTGGGAAGCAC TCGTT-3'
	3'-TATGT AAGTCACAACCTGACCA     GGACTCGTCGTAGGA      TTCCGCGACCCTTCGTG AGCAA-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 008-080224511
Forward Primer: 5'-GGGTAGCGACTCTGCAGACA-3' Tm: 59.508
Reverse Primer: 5'-GTGACTGTGGTGAGCTGGAG-3' Tm: 59.508
Expected PCR Product Length: 333
Expected CelI Cleavage Product Sizes: 196, 137
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr8:80224681-80224729

Rank: 13	Homology Score: 10	TALEN Score: 58.3
	Genomic Region: Intergenic	Closest Gene: MGC125002
Match Type: R23L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-TTTGA TCCAGAATTGTTCTGGT CTAAAAGAAATGTGGTGATAAAA  ATGGAGCAGAGATTGAA GGAAA-3'
	3'-AAACT AGGTCTTAACAAGACCA GATTTTCTTTACACCACTATTTT  TACCTCGTCTCTAACTT CCTTT-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 005-113632907
Forward Primer: 5'-GGAGGAATCTCTAGAAAGTCCCAG-3' Tm: 59.038
Reverse Primer: 5'-GGCTCCTATCTGCAGGTCCA-3' Tm: 59.508
Expected PCR Product Length: 339
Expected CelI Cleavage Product Sizes: 208, 131
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr5:113633085-113633141

Rank: 14	Homology Score: 5	TALEN Score: 58.03
	Genomic Region: Intron	Closest Gene: Top1
Match Type: L21R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-TGGAC TTCCTGCACCACTCAAT  GGCTGTGATTATCAGCACAAG   ACATGAACAATATTGAG CCTGT-3'
	3'-ACCTG AAGGACGTGGTGAGTTA  CCGACACTAATAGTCGTGTTC   TGTACTTGTTATAACTC GGACA-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 003-151465311
Forward Primer: 5'-CACACACACACACACACACACAC-3' Tm: 59.148
Reverse Primer: 5'-CACCAGTGACAACAGCACTTGAG-3' Tm: 59.148
Expected PCR Product Length: 330
Expected CelI Cleavage Product Sizes: 186, 144
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr3:151465468-151465522

Rank: 15	Homology Score: 13	TALEN Score: 58.03
	Genomic Region: Intergenic	Closest Gene: Atpbd4
Match Type: R15L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-CATAA TTCAATGTTGTTTGGGC     TTTTTTTTTTTTTTT      TTGGAGCTGGGGACGAA CCCAG-3'
	3'-GTATT AAGTTACAACAAACCCG     AAAAAAAAAAAAAAA      AACCTCGACCCCTGCTT GGGTC-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 003-100952790
Forward Primer: 5'-GCCATGAAGAAGACATGCAGGAG-3' Tm: 59.148
Reverse Primer: 5'-GGATCACTTGAAATGTGGAGCTTCCTAC-3' Tm: 60.298
Expected PCR Product Length: 405
Expected CelI Cleavage Product Sizes: 218, 187
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr3:100952982-100953030

Rank: 16	Homology Score: 10	TALEN Score: 57.96
	Genomic Region: Intergenic	Closest Gene: Map2
Match Type: R13L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-ATTTA TTCATTGTTGTTCTTGG      TGGCTGCGTTATC       AGTGTTCTGGTCAGGAA GTTGT-3'
	3'-TAAAT AAGTAACAACAAGAACC      ACCGACGCAATAG       TCACAAGACCAGTCCTT CAACA-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 009-065106893
Forward Primer: 5'-CCTCTGTTGGATGTGGAGTTGG-3' Tm: 59.258
Reverse Primer: 5'-CGCACACCTATGGACATCTGAG-3' Tm: 59.258
Expected PCR Product Length: 396
Expected CelI Cleavage Product Sizes: 137, 259
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr9:65107005-65107051

Rank: 17	Homology Score: 13	TALEN Score: 57.83
	Genomic Region: Intergenic	Closest Gene: Ifnar1
Match Type: L15R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-CCTTG TTCCTTCCCAGCTGCCT     AGGAATGTGGGACCA      ACCTGAGCAACACAGCA AGAGC-3'
	3'-GGAAC AAGGAAGGGTCGACGGA     TCCTTACACCCTGGT      TGGACTCGTTGTGTCGT TCTCG-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 011-031478392
Forward Primer: 5'-GATCTGATCCCCTTTTCTGGTGTG-3' Tm: 59.038
Reverse Primer: 5'-CTTCTTTAGCACGTCGCCCC-3' Tm: 59.508
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 134, 136
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr11:31478500-31478548

Rank: 18	Homology Score: 10	TALEN Score: 57.71
	Genomic Region: Intergenic	Closest Gene: Plcl2
Match Type: R21L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-ATGTT TTCAGTGTTGCTCCTGT  TGGGTAAAGGAGGAATGTTCC   CTGGATCGGGGCTGGTA TAGTG-3'
	3'-TACAA AAGTCACAACGAGGACA  ACCCATTTCCTCCTTACAAGG   GACCTAGCCCCGACCAT ATCAC-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 009-004673112
Forward Primer: 5'-AATCCACCGAGCCTAGTAACTGC-3' Tm: 59.148
Reverse Primer: 5'-TACACTGCGCTCACACAGCAAG-3' Tm: 59.258
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 135, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr9:4673218-4673272

Rank: 19	Homology Score: 10	TALEN Score: 57.66
	Genomic Region: Intron	Closest Gene: Rnf4
Match Type: L19R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-GTAGA TCCCTCCCCAGCTCCCT   TGTCAGGACCTAGCCAGGT    ACATGATCAACATTCAA GAAAA-3'
	3'-CATCT AGGGAGGGGTCGAGGGA   ACAGTCCTGGATCGGTCCA    TGTACTAGTTGTAAGTT CTTTT-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 014-082098705
Forward Primer: 5'-AGCCAAGGAAGAAACCTGTGCAGC-3' Tm: 60.908
Reverse Primer: 5'-CTGTGGATGGCTAGAGTGTACTCA-3' Tm: 59.038
Expected PCR Product Length: 300
Expected CelI Cleavage Product Sizes: 130, 170
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr14:82098807-82098859

Rank: 20	Homology Score: 10	TALEN Score: 57.66
	Genomic Region: Intron	Closest Gene: Ralgapa1
Match Type: L13R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-TCTCC TTCCTTTCCAGCTCCCT      TCTTTCTTTCATT       ACTAGATATGCACTGAA AGCTG-3'
	3'-AGAGG AAGGAAAGGTCGAGGGA      AGAAAGAAAGTAA       TGATCTATACGTGACTT TCGAC-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 006-075907048
Forward Primer: 5'-GGTGTCCTCATTCACCTCTGACAA-3' Tm: 59.038
Reverse Primer: 5'-GTGTTTGGAACATCCTCTGCGAC-3' Tm: 59.148
Expected PCR Product Length: 363
Expected CelI Cleavage Product Sizes: 189, 174
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr6:75907212-75907258

Rank: 21	Homology Score: 10	TALEN Score: 57.63
	Genomic Region: Intergenic	Closest Gene: Mir532
Match Type: L21R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-TTTTT TTCCTACCCACCCCCAT  GCCTCCCCAGCCTTGGATGAA   ACAGGAACAAAAGTCAA AATTT-3'
	3'-AAAAA AAGGATGGGTGGGGGTA  CGGAGGGGTCGGAACCTACTT   TGTCCTTGTTTTCAGTT TTAAA-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 00X-027254372
Forward Primer: 5'-CCATTATCGCTAGCCTAGCTTACG-3' Tm: 59.038
Reverse Primer: 5'-GACTGGGAATAAGTGGTGTGGTAC-3' Tm: 59.038
Expected PCR Product Length: 301
Expected CelI Cleavage Product Sizes: 167, 134
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chrX:27254510-27254564

Rank: 22	Homology Score: 10	TALEN Score: 57.55
	Genomic Region: Intron	Closest Gene: Tsc2
Match Type: L18R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-TTAGC TACCTGTCCAGCACCAT    GTCTGCCTGGTGCCTCCC    ACCATGACAATAATGGA CTAAA-3'
	3'-AATCG ATGGACAGGTCGTGGTA    CAGACGGACCACGGAGGG    TGGTACTGTTATTACCT GATTT-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 010-013862379
Forward Primer: 5'-CATAGGTGGGGCCTTATCAGAG-3' Tm: 59.258
Reverse Primer: 5'-GGTCCCTAGTCAAGGACATTGTCT-3' Tm: 59.038
Expected PCR Product Length: 273
Expected CelI Cleavage Product Sizes: 131, 142
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr10:13862482-13862533

Rank: 23	Homology Score: 13	TALEN Score: 57.47
	Genomic Region: Intergenic	Closest Gene: Fgfr2
Match Type: L22R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-TTCTG CTCCACCCCAGCTCCAA  AGGAAATGGCTGCCCATAAATC  ACCAGAAAAACACCAAA GTCCC-3'
	3'-AAGAC GAGGTGGGGTCGAGGTT  TCCTTTACCGACGGGTATTTAG  TGGTCTTTTTGTGGTTT CAGGG-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 001-189623849
Forward Primer: 5'-TGCATTCCCACCCCCATACTG-3' Tm: 59.378
Reverse Primer: 5'-CTGAGCACATTCAGGCATCTGG-3' Tm: 59.258
Expected PCR Product Length: 297
Expected CelI Cleavage Product Sizes: 131, 166
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr1:189623950-189624005

Rank: 24	Homology Score: 10	TALEN Score: 57.43
	Genomic Region: Intergenic	Closest Gene: Zfand6
Match Type: R15L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-AGGAG TTCAGTCATGTTCAAGG     GCACTTCTTGTGTAA      ATGGGGCTGGACAGGGA GGAGT-3'
	3'-TCCTC AAGTCAGTACAAGTTCC     CGTGAAGAACACATT      TACCCCGACCTGTCCCT CCTCA-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 001-140972716
Forward Primer: 5'-GGGACCACCTCAGCTAAGGA-3' Tm: 59.508
Reverse Primer: 5'-GTGGGGTTGTTGGTTTTCAGCTC-3' Tm: 59.148
Expected PCR Product Length: 368
Expected CelI Cleavage Product Sizes: 173, 195
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr1:140972863-140972911

Rank: 25	Homology Score: 8	TALEN Score: 57.33
	Genomic Region: Intergenic	Closest Gene: Actrt1
Match Type: R14L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-ATTTA TTAATTGTTGGTCTGAT      TACCTATGTTATTA      ATGGTTCTGTTCAGGAA GTTTT-3'
	3'-TAAAT AATTAACAACCAGACTA      ATGGATACAATAAT      TACCAAGACAAGTCCTT CAAAA-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Warning! Primer pair was generated using suboptimal parameters. 
Primer Index: 00X-131557987
Forward Primer: 5'-TTTTGGATGTCAGCTATAGGGTTGGAGAAAATCTT-3' Tm: 59.578
Reverse Primer: 5'-CAACAAATGATGCTAATCTAACTGGATGTCTACATG-3' Tm: 59.498
Expected PCR Product Length: 337
Expected CelI Cleavage Product Sizes: 131, 206
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chrX:131558092-131558139

Rank: 26	Homology Score: 13	TALEN Score: 57.33
	Genomic Region: Intergenic	Closest Gene: Robo1
Match Type: L19R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-CTGTA GTCCTACCCAGCTTCAT   TGTCTATGACACAACGATA    ACCAGCACAGCACAGTA ACCTG-3'
	3'-GACAT CAGGATGGGTCGAAGTA   ACAGATACTGTGTTGCTAT    TGGTCGTGTCGTGTCAT TGGAC-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 011-011914436
Forward Primer: 5'-GACACAGTACTCAGAGGATCCAAG-3' Tm: 59.038
Reverse Primer: 5'-GCTTGGCTTCTAGGACCAGAC-3' Tm: 59.378
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 130, 140
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr11:11914538-11914590

Rank: 27	Homology Score: 10	TALEN Score: 57.26
	Genomic Region: Intergenic	Closest Gene: Pde4b
Match Type: R15L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-TTTCG TCCATTATTGTCATGGT     GTCATGCAAGGTGAC      ATGGTGCTGGGGAGGCA GCTGA-3'
	3'-AAAGC AGGTAATAACAGTACCA     CAGTACGTTCCACTG      TACCACGACCCCTCCGT CGACT-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 005-123636672
Forward Primer: 5'-CTCTGAGTGACACGAATTAGTGGG-3' Tm: 59.038
Reverse Primer: 5'-GGAGTGTGTCACTAAGGCTGG-3' Tm: 59.378
Expected PCR Product Length: 350
Expected CelI Cleavage Product Sizes: 218, 132
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr5:123636864-123636912

Rank: 28	Homology Score: 10	TALEN Score: 57.24
	Genomic Region: Intergenic	Closest Gene: Dpp6
Match Type: R15L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-GTCAG TTCTGTGCTGTGCTCAT     GCCATTGTGGGGAAG      AAGGAGATGGGAAGGAA GGTGC-3'
	3'-CAGTC AAGACACGACACGAGTA     CGGTAACACCCCTTC      TTCCTCTACCCTTCCTT CCACG-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 004-003843423
Forward Primer: 5'-GGTGACCAATACCAACCATCAGG-3' Tm: 59.148
Reverse Primer: 5'-CCTCCTTTCAGGAAGGCTCTTTCT-3' Tm: 59.038
Expected PCR Product Length: 319
Expected CelI Cleavage Product Sizes: 169, 150
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr4:3843566-3843614

Rank: 29	Homology Score: 10	TALEN Score: 57.2
	Genomic Region: Intergenic	Closest Gene: Fundc1
Match Type: L19R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-AGACC TTCCTAACCCTTTCCAT   TTGCTTAAATTAACAGAGA    AACAGAACAATACTCAA AATTG-3'
	3'-TCTGG AAGGATTGGGAAAGGTA   AACGAATTTAATTGTCTCT    TTGTCTTGTTATGAGTT TTAAC-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 00X-016509931
Forward Primer: 5'-CTACCAATGTCTATGGAGTGACCG-3' Tm: 59.038
Reverse Primer: 5'-GCCTAGAGTATCCCAAATGTGCAC-3' Tm: 59.038
Expected PCR Product Length: 365
Expected CelI Cleavage Product Sizes: 130, 235
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chrX:16510033-16510085

Rank: 30	Homology Score: 5	TALEN Score: 57.17
	Genomic Region: Intergenic	Closest Gene: Ccnc
Match Type: R15L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-TCATA CTCAAGGTTGTTCTGAT     GCAAACAACCTCCTC      ATTGTTCTGGGTAGTAA ACAGA-3'
	3'-AGTAT GAGTTCCAACAAGACTA     CGTTTGTTGGAGGAG      TAACAAGACCCATCATT TGTCT-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 005-036123844
Forward Primer: 5'-GGCTGTAGACACTCTTCAGCTTG-3' Tm: 59.148
Reverse Primer: 5'-GTGACCTCTCTGCAGACAGC-3' Tm: 59.508
Expected PCR Product Length: 346
Expected CelI Cleavage Product Sizes: 140, 206
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr5:36123958-36124006

Rank: 31	Homology Score: 10	TALEN Score: 56.97
	Genomic Region: Intergenic	Closest Gene: Ttbk2
Match Type: L14R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-GTGTT GCCACGCCCAGCTCCAC      TTTCTAACCTCTAT      ATCAGGACAACACTTAA TAAAT-3'
	3'-CACAA CGGTGCGGGTCGAGGTG      AAAGATTGGAGATA      TAGTCCTGTTGTGAATT ATTTA-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 003-107514838
Forward Primer: 5'-GTTGGCTTAAGGCTGCCAACTTC-3' Tm: 59.148
Reverse Primer: 5'-GTGTGTTGCCTCCTTTTCTACACG-3' Tm: 59.038
Expected PCR Product Length: 440
Expected CelI Cleavage Product Sizes: 234, 206
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr3:107515046-107515093

Rank: 32	Homology Score: 8	TALEN Score: 56.95
	Genomic Region: Intron	Closest Gene: Grm8
Match Type: L13R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-ACCCT TGCCTTCCCTGCTCCTT      CTTCTCTGACTGT       TCTAGCACAGCACTGAA AAAGA-3'
	3'-TGGGA ACGGAAGGGACGAGGAA      GAAGAGACTGACA       AGATCGTGTCGTGACTT TTTCT-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 004-054569528
Forward Primer: 5'-CTGTGAGCAAGCCAACAGATAGC-3' Tm: 59.148
Reverse Primer: 5'-CCTTGGAGTTGCTACAGGCTTTG-3' Tm: 59.148
Expected PCR Product Length: 273
Expected CelI Cleavage Product Sizes: 135, 138
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr4:54569638-54569684

Rank: 33	Homology Score: 13	TALEN Score: 56.87
	Genomic Region: Intergenic	Closest Gene: Fgf13
Match Type: L18R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-CCTCG TTCCTGCTCTTCTCCAT    GGACCACTTTGATGAGAT    AAAAGAACAACACCCAA ACTGG-3'
	3'-GGAGC AAGGACGAGAAGAGGTA    CCTGGTGAAACTACTCTA    TTTTCTTGTTGTGGGTT TGACC-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 00X-144712285
Forward Primer: 5'-CCAAGCGAGACAGAGATCGG-3' Tm: 59.508
Reverse Primer: 5'-GGTACTTGGCCCTCAGAATCG-3' Tm: 59.378
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 135, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chrX:144712392-144712443

Rank: 34	Homology Score: 5	TALEN Score: 56.85
	Genomic Region: Intergenic	Closest Gene: Fyn
Match Type: R14L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-GGAGA TCCAGTTTTCTTCTTGT      CAGGGGTGGGTGGG      ATGGGGTGGGGTAGGTA GGAGA-3'
	3'-CCTCT AGGTCAAAAGAAGAACA      GTCCCCACCCACCC      TACCCCACCCCATCCAT CCTCT-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. Warning! The sequence of this region of the genome is poorly mapped. 
Primer Index: 020-043706993
Forward Primer: 5'-GCAGAAATGGATAGTTTGTGGGGG-3' Tm: 59.038
Reverse Primer: 5'-CCGCCAAGCCCTTTTTAAGTACTG-3' Tm: 59.038
Expected PCR Product Length: 378
Expected CelI Cleavage Product Sizes: 248, 130
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr20:43707215-43707262

Rank: 35	Homology Score: 10	TALEN Score: 56.83
	Genomic Region: Intergenic	Closest Gene: Tacstd2
Match Type: L24R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-TTTCC TTCATTCTCTGCTTCAT TCATTGTGTCTGCATTTCCTTTAG ACAGGAACAACTCTGAA TTATT-3'
	3'-AAAGG AAGTAAGAGACGAAGTA AGTAACACAGACGTAAAGGAAATC TGTCCTTGTTGAGACTT AATAA-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 004-100499585
Forward Primer: 5'-GGCATAGCCTCTCAGGAAACAG-3' Tm: 59.258
Reverse Primer: 5'-CTTCCAGGACAGAACAGGAAAGAC-3' Tm: 59.038
Expected PCR Product Length: 336
Expected CelI Cleavage Product Sizes: 165, 171
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr4:100499719-100499776

Rank: 36	Homology Score: 10	TALEN Score: 56.7
	Genomic Region: Intergenic	Closest Gene: Zfp385b
Match Type: L19R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-TTTCC TTCCAGCCCATCTCCAC   TTCTTTATGCCAGCACTGA    TCCACAGAAACACTGTA AACCA-3'
	3'-AAAGG AAGGTCGGGTAGAGGTG   AAGAAATACGGTCGTGACT    AGGTGTCTTTGTGACAT TTGGT-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 003-060396362
Forward Primer: 5'-CCACTGGGAGTGTATTCGGTATG-3' Tm: 59.148
Reverse Primer: 5'-GTAAACAGGCCTCTACAGAACGG-3' Tm: 59.148
Expected PCR Product Length: 362
Expected CelI Cleavage Product Sizes: 230, 132
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr3:60396564-60396616

Rank: 37	Homology Score: 13	TALEN Score: 56.64
	Genomic Region: Intron	Closest Gene: Ppm1l
Match Type: L12R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-ACTCT TTCCTCCCAAGCTGCTT       TTGGGCGGTTTT       ACCACAATAACAGTGAA GCAAA-3'
	3'-TGAGA AAGGAGGGTTCGACGAA       AACCCGCCAAAA       TGGTGTTATTGTCACTT CGTTT-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 002-159432579
Forward Primer: 5'-GCTAAATCCCCAACCCTGCAG-3' Tm: 59.378
Reverse Primer: 5'-GTGTTTACTTGCTCAAACACCCGG-3' Tm: 59.038
Expected PCR Product Length: 284
Expected CelI Cleavage Product Sizes: 154, 130
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr2:159432708-159432753

Rank: 38	Homology Score: 5	TALEN Score: 56.64
	Genomic Region: Intergenic	Closest Gene: Ppp4r2
Match Type: R15L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-TAAAT TTAAATTTTTTTCTGGA     GGGTGATCACAGGGG      TTGGAGTGGGGCAGGCA CAGCA-3'
	3'-ATTTA AATTTAAAAAAAGACCT     CCCACTAGTGTCCCC      AACCTCACCCCGTCCGT GTCGT-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 004-135905706
Forward Primer: 5'-GACAACGACTGCGTTCTATTTGGG-3' Tm: 59.038
Reverse Primer: 5'-TCACGAGCCCTGGTTATTGTTTGG-3' Tm: 59.038
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 134, 136
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr4:135905814-135905862

Rank: 39	Homology Score: 10	TALEN Score: 56.45
	Genomic Region: Intron	Closest Gene: Sorcs1
Match Type: L23R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-TGATG CTGCTGCTCAACTCCCT TGCTAACAACTGAATACATCTGT  ATCAGAACAACAGTGAG CAAAA-3'
	3'-ACTAC GACGACGAGTTGAGGGA ACGATTGTTGACTTATGTAGACA  TAGTCTTGTTGTCACTC GTTTT-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 001-256112389
Forward Primer: 5'-CCTGGATAAGTCAGTAAAGGGTGG-3' Tm: 59.038
Reverse Primer: 5'-CCTTGGTGGGACCTGATACAGT-3' Tm: 59.258
Expected PCR Product Length: 353
Expected CelI Cleavage Product Sizes: 204, 149
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr1:256112563-256112619

Rank: 40	Homology Score: 5	TALEN Score: 56.44
	Genomic Region: Intron	Closest Gene: Pitpnm2
Match Type: R21L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-CCCTG ATCAGTGCAGTTCTAGT  GGACCAGGGAGGGGTGTGCCA   AGTGTGTTGGACAGGAA CAGAA-3'
	3'-GGGAC TAGTCACGTCAAGATCA  CCTGGTCCCTCCCCACACGGT   TCACACAACCTGTCCTT GTCTT-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 012-033556886
Forward Primer: 5'-TCTGTCTGCAAGCTCCAGGG-3' Tm: 59.508
Reverse Primer: 5'-AACTCTGGCCTCCTTGGTCC-3' Tm: 59.508
Expected PCR Product Length: 277
Expected CelI Cleavage Product Sizes: 139, 138
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr12:33556996-33557050

Rank: 41	Homology Score: 8	TALEN Score: 56.35
	Genomic Region: Intergenic	Closest Gene: Rps29
Match Type: L16R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-GCACA TTCCTCCACATTTCCAT     CTCGAGGAAAGATTGG     ATCAGATCAAGAGTGAA ACTCA-3'
	3'-CGTGT AAGGAGGTGTAAAGGTA     GAGCTCCTTTCTAACC     TAGTCTAGTTCTCACTT TGAGT-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 006-090557385
Forward Primer: 5'-GTCCCCACAGGCATATGATCG-3' Tm: 59.378
Reverse Primer: 5'-GTGCTTCGGGCAGTAGAGCT-3' Tm: 59.508
Expected PCR Product Length: 348
Expected CelI Cleavage Product Sizes: 217, 131
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr6:90557575-90557624

Rank: 42	Homology Score: 10	TALEN Score: 56.34
	Genomic Region: Exon	Closest Gene: Lrp1
Match Type: R20L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-GCTGT TTCAATCTTGGGGTGGT   TCCCCCAGTCTGACCAGTAC   ATGGTGCTGGGAAGGGA GAGAT-3'
	3'-CGACA AAGTTAGAACCCCACCA   AGGGGGTCAGACTGGTCATG   TACCACGACCCTTCCCT CTCTA-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 007-067524783
Forward Primer: 5'-TGATCAGAGCCTGCCCCTTC-3' Tm: 59.508
Reverse Primer: 5'-GACAGGGTGCTTCCTCACAATAC-3' Tm: 59.148
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 138, 132
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr7:67524892-67524945

Rank: 43	Homology Score: 10	TALEN Score: 56.31
	Genomic Region: Intergenic	Closest Gene: Rn45s
Match Type: L23R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-GGATC TGCCTTCTCAGATCCAA ATCCTCCATTTCAAGATGCAAGA  AGCAGAGCCACACTGAA AGCTG-3'
	3'-CCTAG ACGGAAGAGTCTAGGTT TAGGAGGTAAAGTTCTACGTTCT  TCGTCTCGGTGTGACTT TCGAC-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 0Un-021787610
Forward Primer: 5'-CCTGGGATGTGGTGTAAGTTGG-3' Tm: 59.258
Reverse Primer: 5'-GAGGAGCCAAATCTGGACCATTAG-3' Tm: 59.038
Expected PCR Product Length: 273
Expected CelI Cleavage Product Sizes: 138, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chrUn:21787718-21787774

Rank: 44	Homology Score: 10	TALEN Score: 56.31
	Genomic Region: Intergenic	Closest Gene: Cav2
Match Type: L23R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-GGATC TGCCTTCTCAGATCCAA ATCCTCCTTTTCAAGATGCAAGA  AGCAGAGCCACACTGAA AGCTG-3'
	3'-CCTAG ACGGAAGAGTCTAGGTT TAGGAGGAAAAGTTCTACGTTCT  TCGTCTCGGTGTGACTT TCGAC-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: dom-003376644
Forward Primer: 5'-CCTGGGATGTGGTGTAAGTTGG-3' Tm: 59.258
Reverse Primer: 5'-GAGGAGCCAAATCTGGACCATTAG-3' Tm: 59.038
Expected PCR Product Length: 273
Expected CelI Cleavage Product Sizes: 138, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chrUn_random:3376752-3376808

Rank: 45	Homology Score: 10	TALEN Score: 56.31
	Genomic Region: Intergenic	Closest Gene: LOC367515
Match Type: L23R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-GGATC TGCCTTCTCAGATCCAA ATCCTCCATTTCAAGATGCAAGA  AGCAGAGCCACACTGAA AGCTG-3'
	3'-CCTAG ACGGAAGAGTCTAGGTT TAGGAGGTAAAGTTCTACGTTCT  TCGTCTCGGTGTGACTT TCGAC-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. Warning! The sequence of this region of the genome is poorly mapped. 
Primer Index: 0Un-038608458
Forward Primer: 5'-CCTGGGATGTGGTGTAAGTTGG-3' Tm: 59.258
Reverse Primer: 5'-GAGACAACATACCCTGCTCTCAC-3' Tm: 59.148
Expected PCR Product Length: 316
Expected CelI Cleavage Product Sizes: 138, 178
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chrUn:38608566-38608622

Rank: 46	Homology Score: 10	TALEN Score: 56.31
	Genomic Region: Intergenic	Closest Gene: Adrbk2
Match Type: L23R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-GGATC TGCCTTCTCAGATCCAA ATCCTCCATTTCAAGATGCAAGA  AGCAGAGCCACACTGAA AGCTG-3'
	3'-CCTAG ACGGAAGAGTCTAGGTT TAGGAGGTAAAGTTCTACGTTCT  TCGTCTCGGTGTGACTT TCGAC-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 0Un-051334472
Forward Primer: 5'-CCTGGGATGTGGTGTAAGTTGG-3' Tm: 59.258
Reverse Primer: 5'-GAGGAGCCAAATCTGGACCATTAG-3' Tm: 59.038
Expected PCR Product Length: 273
Expected CelI Cleavage Product Sizes: 138, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chrUn:51334580-51334636

Rank: 47	Homology Score: 10	TALEN Score: 56.31
	Genomic Region: Intergenic	Closest Gene: LOC302022
Match Type: L23R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-GGATC TGCCTTCTCAGATCCAA ATCCTCCATTTCAAGATGCAAGA  AGCAGAGCCACACTGAA AGCTG-3'
	3'-CCTAG ACGGAAGAGTCTAGGTT TAGGAGGTAAAGTTCTACGTTCT  TCGTCTCGGTGTGACTT TCGAC-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 0Un-061148009
Forward Primer: 5'-CCTGGGATGTGGTGTAAGTTGG-3' Tm: 59.258
Reverse Primer: 5'-GAGACAACATACCCTGCTCTCAC-3' Tm: 59.148
Expected PCR Product Length: 316
Expected CelI Cleavage Product Sizes: 138, 178
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chrUn:61148117-61148173

Rank: 48	Homology Score: 10	TALEN Score: 56.29
	Genomic Region: Intergenic	Closest Gene: Mctp2
Match Type: R14L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-AATCA TTCAGTGAATTTGTTGT      GTCACATCTGCCCC      ATGGAGGTGGGAGGGAA GAATA-3'
	3'-TTAGT AAGTCACTTAAACAACA      CAGTGTAGACGGGG      TACCTCCACCCTCCCTT CTTAT-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 001-127719260
Forward Primer: 5'-CCAACATTCTCCCAAGAGATTCTGGAAAC-3' Tm: 60.178
Reverse Primer: 5'-GTTCCAGGTCAACCAGGACTAC-3' Tm: 59.258
Expected PCR Product Length: 368
Expected CelI Cleavage Product Sizes: 135, 233
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr1:127719369-127719416

Rank: 49	Homology Score: 8	TALEN Score: 56.29
	Genomic Region: Intron	Closest Gene: Map2k1
Match Type: L15R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-CAGCC TTCCTTCCCATCTACAG     AAGAAATCAGCGAAG      AGCAGAACTCCCCTGAA GTCAG-3'
	3'-GTCGG AAGGAAGGGTAGATGTC     TTCTTTAGTCGCTTC      TCGTCTTGAGGGGACTT CAGTC-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 008-068387105
Forward Primer: 5'-CCGACTAGCTTCTCCCATGAC-3' Tm: 59.378
Reverse Primer: 5'-TTACTGTCGGTGTCACAGTGCC-3' Tm: 59.258
Expected PCR Product Length: 322
Expected CelI Cleavage Product Sizes: 191, 131
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr8:68387270-68387318

Rank: 50	Homology Score: 5	TALEN Score: 56.26
	Genomic Region: Intron	Closest Gene: Dcc
Match Type: L20R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-AATTC TTGCTACCCAGCCCAAT   ATGGTATTTATATAAAAATA   ATATGATCAACACTGGA TCTAA-3'
	3'-TTAAG AACGATGGGTCGGGTTA   TACCATAAATATATTTTTAT   TATACTAGTTGTGACCT AGATT-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Warning! Primer pair was generated using suboptimal parameters. 
Primer Index: 018-068645313
Forward Primer: 5'-CTGGGAGCCTGGAAATTTGTCTG-3' Tm: 59.148
Reverse Primer: 5'-GGACACAAGCATATATTATTCTTTGGATGTGCTC-3' Tm: 59.668
Expected PCR Product Length: 439
Expected CelI Cleavage Product Sizes: 177, 262
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr18:68645461-68645514

Rank: 51	Homology Score: 10	TALEN Score: 56.25
	Genomic Region: Promoter	Closest Gene: Zfp830
Match Type: L24R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-ACCTC TGCCTGCCTAGCTCCAA AGCTAGTACCCGCATGTCTGCCAC ATCAAAACAATACTGTC TGCGA-3'
	3'-TGGAG ACGGACGGATCGAGGTT TCGATCATGGGCGTACAGACGGTG TAGTTTTGTTATGACAG ACGCT-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 010-070982895
Forward Primer: 5'-CAGGGTGTGTCTTTCCCAAGATAC-3' Tm: 59.038
Reverse Primer: 5'-GGGTCTCAGGAAACGTTGTGTTG-3' Tm: 59.148
Expected PCR Product Length: 366
Expected CelI Cleavage Product Sizes: 221, 145
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr10:70983085-70983142

Rank: 52	Homology Score: 8	TALEN Score: 56.21
	Genomic Region: Intergenic	Closest Gene: Stk17b
Match Type: R21L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-TGGCA TTCCGTTTGGTCCTGGT  TACTCCTGCTGACTCATTAGA   ATTGAGCTGGCTGGGAA GGTAA-3'
	3'-ACCGT AAGGCAAACCAGGACCA  ATGAGGACGACTGAGTAATCT   TAACTCGACCGACCCTT CCATT-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 009-052013154
Forward Primer: 5'-GAGGCTGAGCTTGGCTTGCTT-3' Tm: 59.378
Reverse Primer: 5'-GCAAGGTCACATAGTGTCACTTGG-3' Tm: 59.038
Expected PCR Product Length: 311
Expected CelI Cleavage Product Sizes: 157, 154
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr9:52013282-52013336

Rank: 53	Homology Score: 5	TALEN Score: 56.14
	Genomic Region: Intergenic	Closest Gene: Grb14
Match Type: R21L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-AAGTG TTCAGAATAGTTCTGAG  TACAATCACATTTAAAAATAG   AGAGAGCGGGACAGGAA CAAAA-3'
	3'-TTCAC AAGTCTTATCAAGACTC  ATGTTAGTGTAAATTTTTATC   TCTCTCGCCCTGTCCTT GTTTT-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 003-046502425
Forward Primer: 5'-GCCAAGGCTAAAGCAGTTTGGAAG-3' Tm: 59.038
Reverse Primer: 5'-GGAGGCACATTCTTAGGCTGCT-3' Tm: 59.258
Expected PCR Product Length: 289
Expected CelI Cleavage Product Sizes: 158, 131
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr3:46502554-46502608

Rank: 54	Homology Score: 5	TALEN Score: 56.13
	Genomic Region: Intergenic	Closest Gene: Rnf217
Match Type: L17R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-AAGAA TTCATACCAAACTCCAT    ATCACCCTGTATATGAC     AACATAGCACCACTAAA AGTCC-3'
	3'-TTCTT AAGTATGGTTTGAGGTA    TAGTGGGACATATACTG     TTGTATCGTGGTGATTT TCAGG-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Warning! Primer pair was generated using suboptimal parameters. 
Primer Index: 001-026132578
Forward Primer: 5'-CATTCAGATACTCTGAAGCGAAACATGTTAAGG-3' Tm: 59.758
Reverse Primer: 5'-CCCCTGACCAATACTTTGCAAACG-3' Tm: 59.038
Expected PCR Product Length: 334
Expected CelI Cleavage Product Sizes: 148, 186
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr1:26132699-26132749

Rank: 55	Homology Score: 5	TALEN Score: 55.92
	Genomic Region: Intron	Closest Gene: Prickle2
Match Type: L13R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-CCTGT TTCCTTCCCAAATCCCC      TGACAAGACATAG       AGCAGAAGAACATTTAA AATTA-3'
	3'-GGACA AAGGAAGGGTTTAGGGG      ACTGTTCTGTATC       TCGTCTTCTTGTAAATT TTAAT-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 004-126653383
Forward Primer: 5'-CCATGTGACCTTTGGACTACATGC-3' Tm: 59.038
Reverse Primer: 5'-GAGAGCCACTGACTACCATAACAC-3' Tm: 59.038
Expected PCR Product Length: 327
Expected CelI Cleavage Product Sizes: 145, 182
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr4:126653503-126653549

Rank: 56	Homology Score: 10	TALEN Score: 55.91
	Genomic Region: Intergenic	Closest Gene: Gabra2
Match Type: R19L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-GCTGT TGCAGTTTAGTATTGGT   ACGGAGGTCCTGGGAAGGC    ATTGCTCTGGGCAGGAA ATAAG-3'
	3'-CGACA ACGTCAAATCATAACCA   TGCCTCCAGGACCCTTCCG    TAACGAGACCCGTCCTT TATTC-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 014-039438747
Forward Primer: 5'-GCTCCATAAACTGGGCCTGAGA-3' Tm: 59.258
Reverse Primer: 5'-GCTAGCCTCTACCTCTTCCTG-3' Tm: 59.378
Expected PCR Product Length: 342
Expected CelI Cleavage Product Sizes: 144, 198
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr14:39438863-39438915

Rank: 57	Homology Score: 10	TALEN Score: 55.9
	Genomic Region: Intron	Closest Gene: Fra10ac1
Match Type: L22R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-TCAAG TTCCTAGCCAGCTTCAT  TCACAGAGCAAGTTCCAGGACA  GCCAGGGCTACACAGAA AAACT-3'
	3'-AGTTC AAGGATCGGTCGAAGTA  AGTGTCTCGTTCAAGGTCCTGT  CGGTCCCGATGTGTCTT TTTGA-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 001-242536006
Forward Primer: 5'-TGGAGGTTGGGAGAGTAGAGG-3' Tm: 59.378
Reverse Primer: 5'-TGAAGGAGGGAACACTCAGCAG-3' Tm: 59.258
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 135, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr1:242536111-242536166

Rank: 58	Homology Score: 2	TALEN Score: 55.85
	Genomic Region: Intron	Closest Gene: LOC246295
Match Type: L20R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-TGTGC TTTCCACACAGCACCAT   GCAGCCTCAGTCACCAGGCC   TCTAGAACAGCATTGTA ACAGG-3'
	3'-ACACG AAAGGTGTGTCGTGGTA   CGTCGGAGTCAGTGGTCCGG   AGATCTTGTCGTAACAT TGTCC-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 005-170773963
Forward Primer: 5'-CCAAGGTTGCTGCCAGTTCAGCT-3' Tm: 61.088
Reverse Primer: 5'-AGGAGCTGGAGAGCCAAGTG-3' Tm: 59.508
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 135, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr5:170774069-170774122

Rank: 59	Homology Score: 10	TALEN Score: 55.83
	Genomic Region: Intergenic	Closest Gene: Cntnap5b
Match Type: L22R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-CTCTT TCTCTTCCTAGCTCCCT  GCAATTGTATCCTGCTGTGAGT  ACCAGATCAAAAGTGAA TATTT-3'
	3'-GAGAA AGAGAAGGATCGAGGGA  CGTTAACATAGGACGACACTCA  TGGTCTAGTTTTCACTT ATAAA-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 013-002441765
Forward Primer: 5'-CTGTCTCTGTCTCTCTTAGTTCCC-3' Tm: 59.038
Reverse Primer: 5'-CCCTTTTTGCCTTTCCATGCAGTG-3' Tm: 59.038
Expected PCR Product Length: 336
Expected CelI Cleavage Product Sizes: 177, 159
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr13:2441912-2441967

Rank: 60	Homology Score: 8	TALEN Score: 55.82
	Genomic Region: Intergenic	Closest Gene: Lamp5
Match Type: L24R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-ATACC TTCCACCCCACCTCCTT CCCCTAGATTTCAAGAAGGGGGGA AGCAGAACAAAACCTAA CCTCG-3'
	3'-TATGG AAGGTGGGGTGGAGGAA GGGGATCTAAAGTTCTTCCCCCCT TCGTCTTGTTTTGGATT GGAGC-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 003-124119953
Forward Primer: 5'-CAGTAGCTGCCTTTGACCAGGA-3' Tm: 59.258
Reverse Primer: 5'-CAAACCAGACAATGGCTTCTGCTC-3' Tm: 59.038
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 140, 130
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr3:124120062-124120119

Rank: 61	Homology Score: 8	TALEN Score: 55.78
	Genomic Region: Intergenic	Closest Gene: Sfrp2
Match Type: L13R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-TCATG TACCAGCCCAGCTGCAG      TCCAGCTTCTATG       TCCAGCACTGCACTGAA ACTGT-3'
	3'-AGTAC ATGGTCGGGTCGACGTC      AGGTCGAAGATAC       AGGTCGTGACGTGACTT TGACA-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 002-175409656
Forward Primer: 5'-CATCGGGCAGGGTTTCAAAGC-3' Tm: 59.378
Reverse Primer: 5'-GCCCAAGCTGACATTTGAGAGAC-3' Tm: 59.148
Expected PCR Product Length: 303
Expected CelI Cleavage Product Sizes: 159, 144
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr2:175409790-175409836

Rank: 62	Homology Score: 5	TALEN Score: 55.75
	Genomic Region: Intergenic	Closest Gene: Slc15a5
Match Type: L20R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-CGTTA TTAATGCACAGCCCCAT   AAAAACATTGATTTAGAGGA   ACCTTTATAACATTGAA GTGCA-3'
	3'-GCAAT AATTACGTGTCGGGGTA   TTTTTGTAACTAAATCTCCT   TGGAAATATTGTAACTT CACGT-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 004-174994043
Forward Primer: 5'-GTGCGGCTCTGTCCTCACAT-3' Tm: 59.508
Reverse Primer: 5'-GATTAAATTGCATGCAGCTGCACTAACTCC-3' Tm: 60.058
Expected PCR Product Length: 269
Expected CelI Cleavage Product Sizes: 138, 131
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr4:174994152-174994205

Rank: 63	Homology Score: 5	TALEN Score: 55.74
	Genomic Region: Intergenic	Closest Gene: Hmg20a
Match Type: L19R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-TTCAT TTCATGAACAGCTACTT   GGCGCTCTGTCTTCTAGTG    ATCAGTAAAACACGGAA GTCTT-3'
	3'-AAGTA AAGTACTTGTCGATGAA   CCGCGAGACAGAAGATCAC    TAGTCATTTTGTGCCTT CAGAA-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 008-060163371
Forward Primer: 5'-TGGTCAATGTCCTTGGGGCTG-3' Tm: 59.378
Reverse Primer: 5'-CGAGTCTTTCTAGCCAGTCAAGTG-3' Tm: 59.038
Expected PCR Product Length: 279
Expected CelI Cleavage Product Sizes: 130, 149
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr8:60163473-60163525

Rank: 64	Homology Score: 5	TALEN Score: 55.74
	Genomic Region: Intergenic	Closest Gene: Lingo1
Match Type: L19R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-TTCAT TTCATGAACAGCTACTT   GGCGCTCTGTCTTCTAGTG    ATCAGTAAAACACGGAA GTCTT-3'
	3'-AAGTA AAGTACTTGTCGATGAA   CCGCGAGACAGAAGATCAC    TAGTCATTTTGTGCCTT CAGAA-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 008-060289566
Forward Primer: 5'-TGGTCAATGTCCTTGGGGCTG-3' Tm: 59.378
Reverse Primer: 5'-CGAGTCTTTCTAGCCAGTCAAGTG-3' Tm: 59.038
Expected PCR Product Length: 279
Expected CelI Cleavage Product Sizes: 130, 149
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr8:60289668-60289720

Rank: 65	Homology Score: 13	TALEN Score: 55.73
	Genomic Region: Intron	Closest Gene: Usp30
Match Type: R24L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-CCCAG TTCAGTGTTGGGATGGT GGCAGGATACACAGAGGCTTGGCA GTGCAGCTGGGGAGGAT GTGTG-3'
	3'-GGGTC AAGTCACAACCCTACCA CCGTCCTATGTGTCTCCGAACCGT CACGTCGACCCCTCCTA CACAC-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 012-043552977
Forward Primer: 5'-CACCCACTTCATGTTGGAAACGC-3' Tm: 59.148
Reverse Primer: 5'-TTCCAAACCCTCTGCCCTGC-3' Tm: 59.508
Expected PCR Product Length: 278
Expected CelI Cleavage Product Sizes: 131, 147
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr12:43553077-43553134

Rank: 66	Homology Score: 10	TALEN Score: 55.67
	Genomic Region: Intergenic	Closest Gene: Tbl1xr1
Match Type: R22L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-ACATC TCCAGTCTTATTCTGGT  CTATTATCAACAGTAAGGGAAA  ATGAAGCTGCCATGGAA AGTAC-3'
	3'-TGTAG AGGTCAGAATAAGACCA  GATAATAGTTGTCATTCCCTTT  TACTTCGACGGTACCTT TCATG-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 002-106965669
Forward Primer: 5'-ATAGTGGCCAGTGCCTGGAC-3' Tm: 59.508
Reverse Primer: 5'-CCAGGAGAAAAGGCCATCTTCCA-3' Tm: 59.148
Expected PCR Product Length: 307
Expected CelI Cleavage Product Sizes: 131, 176
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr2:106965770-106965825

Rank: 67	Homology Score: 10	TALEN Score: 55.65
	Genomic Region: Intron	Closest Gene: Tgfbr1
Match Type: L12R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-AAAAT ATCCTTCCAGGCTTCAT       TAAGTAACTTAG       ACCAGATAAACACTTAA CCAAG-3'
	3'-TTTTA TAGGAAGGTCCGAAGTA       ATTCATTGAATC       TGGTCTATTTGTGAATT GGTTC-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 005-063999032
Forward Primer: 5'-CAGGAGCAAGACAAGAGTGTCTAC-3' Tm: 59.038
Reverse Primer: 5'-CGGTTAGTGTGGGATGTCTTTCTC-3' Tm: 59.038
Expected PCR Product Length: 361
Expected CelI Cleavage Product Sizes: 138, 223
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr5:63999145-63999190

Rank: 68	Homology Score: 10	TALEN Score: 55.65
	Genomic Region: Intergenic	Closest Gene: Irx1
Match Type: R22L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-GGTTT TTCTGTGTAGTTCTGGC  TGTCCTGGAACTGAATCAGTAG  ATGAGGCTGGCCTTGAA CTCAA-3'
	3'-CCAAA AAGACACATCAAGACCG  ACAGGACCTTGACTTAGTCATC  TACTCCGACCGGAACTT GAGTT-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 0Un-075352159
Forward Primer: 5'-CTTGAGAGACTGTGGTGAATGAGG-3' Tm: 59.038
Reverse Primer: 5'-GGAGGAAAAAGGGGACTGTGTTCA-3' Tm: 59.038
Expected PCR Product Length: 298
Expected CelI Cleavage Product Sizes: 150, 148
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chrUn:75352279-75352334

Rank: 69	Homology Score: 10	TALEN Score: 55.65
	Genomic Region: Intergenic	Closest Gene: Grid2
Match Type: R22L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-GGTTT TTCTGTGTAGTTCTGGC  TGTCCTGGAACTGAATCAGTAG  ATGAGGCTGGCCTTGAA CTCAA-3'
	3'-CCAAA AAGACACATCAAGACCG  ACAGGACCTTGACTTAGTCATC  TACTCCGACCGGAACTT GAGTT-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 004-092024652
Forward Primer: 5'-CTTGAGAGACTGTGGTGAATGAGG-3' Tm: 59.038
Reverse Primer: 5'-GGAGGAAAAAGGGGACTGTGTTCA-3' Tm: 59.038
Expected PCR Product Length: 298
Expected CelI Cleavage Product Sizes: 150, 148
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr4:92024772-92024827

Rank: 70	Homology Score: 5	TALEN Score: 55.61
	Genomic Region: Intergenic	Closest Gene: Tasp1
Match Type: R14L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-TGTCT TTCAGTTTTGTTGTACT      GAGAGAGAAAGAGA      ATGAAGTTGGGTGGGTA GGAGG-3'
	3'-ACAGA AAGTCAAAACAACATGA      CTCTCTCTTTCTCT      TACTTCAACCCACCCAT CCTCC-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 003-128243778
Forward Primer: 5'-CAACCACACTGCAGACCAGG-3' Tm: 59.508
Reverse Primer: 5'-GCCATGGATCTGATATTGGTGGCT-3' Tm: 59.038
Expected PCR Product Length: 308
Expected CelI Cleavage Product Sizes: 131, 177
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr3:128243883-128243930

Rank: 71	Homology Score: 10	TALEN Score: 55.58
	Genomic Region: Intergenic	Closest Gene: Uncx
Match Type: L23R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-GAGTC TTTCTGCCCAGCTCTAG CTGTCCTGGAACTCACCCTGTAG  ACCAGGCCAGCCTTGAA CTCAC-3'
	3'-CTCAG AAAGACGGGTCGAGATC GACAGGACCTTGAGTGGGACATC  TGGTCCGGTCGGAACTT GAGTG-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 012-015478690
Forward Primer: 5'-CCACTCAAGACAAGCAGAAAGGAC-3' Tm: 59.038
Reverse Primer: 5'-CAAAACAGCAGCAGCAAAAGCTGG-3' Tm: 59.038
Expected PCR Product Length: 278
Expected CelI Cleavage Product Sizes: 147, 131
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr12:15478807-15478863

Rank: 72	Homology Score: 8	TALEN Score: 55.53
	Genomic Region: Intergenic	Closest Gene: Clec14a
Match Type: L17R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-AGGTC TTGCTGCCCATCTTCAA    TAAGCCAAGAGAAAAGA     ACCAGCTTAACAGTGAA AAGTA-3'
	3'-TCCAG AACGACGGGTAGAAGTT    ATTCGGTTCTCTTTTCT     TGGTCGAATTGTCACTT TTCAT-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 006-079162173
Forward Primer: 5'-ACACAGAAGGCTCTCCAGAGAC-3' Tm: 59.258
Reverse Primer: 5'-ACTTTGATCTCACTTCAGGTCCCC-3' Tm: 59.038
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 138, 132
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr6:79162284-79162334

Rank: 73	Homology Score: 8	TALEN Score: 55.51
	Genomic Region: Intron	Closest Gene: Bai1
Match Type: R20L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-CCATG TTCTGTGTTGTCTTTGT   TTCTTTGTTGTTAAACTGGG   ATGGGGAGGGGCAGGTA CGCTT-3'
	3'-GGTAC AAGACACAACAGAAACA   AAGAAACAACAATTTGACCC   TACCCCTCCCCGTCCAT GCGAA-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 007-112677493
Forward Primer: 5'-TAGGGCAGAGCTGATGGACC-3' Tm: 59.508
Reverse Primer: 5'-CGAGTCTCTTCTTGACTGTTTCCC-3' Tm: 59.038
Expected PCR Product Length: 274
Expected CelI Cleavage Product Sizes: 137, 137
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr7:112677601-112677654

Rank: 74	Homology Score: 10	TALEN Score: 55.51
	Genomic Region: Intron	Closest Gene: Tm6sf1
Match Type: R13L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-CTTTG TTCAGTTTTTTTTTTTT      TTTTTTTCTTTTT       TTGGAGCTGGGAACGAA CCCAG-3'
	3'-GAAAC AAGTCAAAAAAAAAAAA      AAAAAAAGAAAAA       AACCTCGACCCTTGCTT GGGTC-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. Warning! The sequence of this region of the genome is poorly mapped. 
Primer Index: 001-138061468
Forward Primer: 5'-CCGTGAAGCAGGTCACAAAGC-3' Tm: 59.378
Reverse Primer: 5'-CAGCAAGGGAAGTGCTACTTGATG-3' Tm: 59.038
Expected PCR Product Length: 303
Expected CelI Cleavage Product Sizes: 164, 139
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr1:138061607-138061653

Rank: 75	Homology Score: 5	TALEN Score: 55.45
	Genomic Region: Intergenic	Closest Gene: Tesl
Match Type: R24L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-TCCTA TTTAGTGTTGCTATTGC TGTGGTAAAACACTACAACCAACC ATTGGGTTGGGAAGGAA CGGGT-3'
	3'-AGGAT AAATCACAACGATAACG ACACCATTTTGTGATGTTGGTTGG TAACCCAACCCTTCCTT GCCCA-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 00X-010426535
Forward Primer: 5'-GTTCTCTGCATCCATGCCATGTG-3' Tm: 59.148
Reverse Primer: 5'-CCTCAGCATCAGTTACTGCTTCC-3' Tm: 59.148
Expected PCR Product Length: 383
Expected CelI Cleavage Product Sizes: 252, 131
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chrX:10426756-10426813

Rank: 76	Homology Score: 5	TALEN Score: 55.45
	Genomic Region: Intron	Closest Gene: Myh1
Match Type: L24R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-GTTCT TTCCTGCAAAGATCAAG TAAGTCTTGGAGTCTGTATTGTTA ACAAGATTAACAATGAA GTAGT-3'
	3'-CAAGA AAGGACGTTTCTAGTTC ATTCAGAACCTCAGACATAACAAT TGTTCTAATTGTTACTT CATCA-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 010-053902466
Forward Primer: 5'-CCCATTACCACCCTCCCCTA-3' Tm: 59.508
Reverse Primer: 5'-AGGGAGACAAATGAGGGTTTTGTCCTTC-3' Tm: 60.298
Expected PCR Product Length: 377
Expected CelI Cleavage Product Sizes: 247, 130
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr10:53902682-53902739

Rank: 77	Homology Score: 13	TALEN Score: 55.45
	Genomic Region: Promoter	Closest Gene: Cln5
Match Type: R14L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-AGTGT TTTATTGTTGTTATGGT      TTGGCTGGTGGTGG      CTGCAGGTGGGCAGAAA GCACC-3'
	3'-TCACA AAATAACAACAATACCA      AACCGACCACCACC      GACGTCCACCCGTCTTT CGTGG-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 015-087079625
Forward Primer: 5'-CAATCACTCCCTGCAATCCCC-3' Tm: 59.378
Reverse Primer: 5'-TGACGCCTCCACTGTCCTAG-3' Tm: 59.508
Expected PCR Product Length: 326
Expected CelI Cleavage Product Sizes: 142, 184
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr15:87079741-87079788

Rank: 78	Homology Score: 5	TALEN Score: 55.43
	Genomic Region: Intergenic	Closest Gene: Cyp2r1
Match Type: L24R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-TCTCC TTCAATCTCTGCTCCAT TTCTTGTCCCTGCATTTCCTTTAG ACAAGAACAATTCTGGA TCAAA-3'
	3'-AGAGG AAGTTAGAGACGAGGTA AAGAACAGGGACGTAAAGGAAATC TGTTCTTGTTAAGACCT AGTTT-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 001-172645535
Forward Primer: 5'-CTCCTGTTTGCAAGCACATCTTGG-3' Tm: 59.038
Reverse Primer: 5'-CTGAAATGTCCAACAGTGGGGC-3' Tm: 59.258
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 133, 137
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr1:172645637-172645694

Rank: 79	Homology Score: 10	TALEN Score: 55.42
	Genomic Region: Intergenic	Closest Gene: E2f3
Match Type: R20L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-TTTTG TGCTGTGCTGTGCTGGG   CACAGAACCCAGGGCAGAGG   AGCGTGCTGGGCAGGAA CCCAA-3'
	3'-AAAAC ACGACACGACACGACCC   GTGTCTTGGGTCCCGTCTCC   TCGCACGACCCGTCCTT GGGTT-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 017-041218534
Forward Primer: 5'-CCAGGCCATGATTATTCTGCAGG-3' Tm: 59.148
Reverse Primer: 5'-TCCCAAGTCACCTGCAAGCC-3' Tm: 59.508
Expected PCR Product Length: 343
Expected CelI Cleavage Product Sizes: 212, 131
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr17:41218717-41218770

Rank: 80	Homology Score: 8	TALEN Score: 55.4
	Genomic Region: Intergenic	Closest Gene: Mir100
Match Type: L12R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-AGTAC TTCAAGACCAGCTCCTT       TGAATCGTTCTC       CCCAGAACAAGGCTGCA CCTGA-3'
	3'-TCATG AAGTTCTGGTCGAGGAA       ACTTAGCAAGAG       GGGTCTTGTTCCGACGT GGACT-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 008-044521883
Forward Primer: 5'-CAGAGGACCTGTGAGAGACAGT-3' Tm: 59.258
Reverse Primer: 5'-GAAGCAAATGTGAGAGGAAAAGCCTTGATG-3' Tm: 60.058
Expected PCR Product Length: 439
Expected CelI Cleavage Product Sizes: 308, 131
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr8:44522166-44522211

Rank: 81	Homology Score: 10	TALEN Score: 55.39
	Genomic Region: Intron	Closest Gene: Ube2j1
Match Type: L21R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-TTGCT TTCCTTTCCAGCTCCTT  CTGCCTCTCTTAGGCATTTCC   AGCAGAGCCACTCTGCA ATGGT-3'
	3'-AACGA AAGGAAAGGTCGAGGAA  GACGGAGAGAATCCGTAAAGG   TCGTCTCGGTGAGACGT TACCA-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 005-049320556
Forward Primer: 5'-CTGTGGTGAAGGTGTTACTGTGTC-3' Tm: 59.038
Reverse Primer: 5'-GAGAAGGCTGGACCGAATAGAAG-3' Tm: 59.148
Expected PCR Product Length: 292
Expected CelI Cleavage Product Sizes: 161, 131
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr5:49320688-49320742

Rank: 82	Homology Score: 5	TALEN Score: 55.37
	Genomic Region: Intergenic	Closest Gene: Tmem132b
Match Type: R20L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-GGACC TTCACTGTGATTCTTTT   GTGCATTCAAAAACATCCAC   TTGGGGCTGGGAAGGTA CCTCG-3'
	3'-CCTGG AAGTGACACTAAGAAAA   CACGTAAGTTTTTGTAGGTG   AACCCCGACCCTTCCAT GGAGC-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 012-031851371
Forward Primer: 5'-GGGTGCCTGACTGCTATATTTCC-3' Tm: 59.148
Reverse Primer: 5'-TTGCCTGCCACAACCATGCATC-3' Tm: 59.258
Expected PCR Product Length: 298
Expected CelI Cleavage Product Sizes: 168, 130
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr12:31851510-31851563

Rank: 83	Homology Score: 5	TALEN Score: 55.35
	Genomic Region: Intergenic	Closest Gene: Slc7a11
Match Type: R15L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-TTTGT TTCATTTTTGTTTTTGT     TGTGAGAGACAGAAT      ATGAAGTTGGGTGGGTA ATAAG-3'
	3'-AAACA AAGTAAAAACAAAAACA     ACACTCTCTGTCTTA      TACTTCAACCCACCCAT TATTC-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. Warning! The sequence of this region of the genome is poorly mapped. 
Primer Index: 002-139129605
Forward Primer: 5'-CCCATGTGCAGAAGGAGTTGTAC-3' Tm: 59.148
Reverse Primer: 5'-GTTCCAGGTCAGTCAGGGATATAC-3' Tm: 59.038
Expected PCR Product Length: 358
Expected CelI Cleavage Product Sizes: 175, 183
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr2:139129754-139129802

Rank: 84	Homology Score: 10	TALEN Score: 55.29
	Genomic Region: Intergenic	Closest Gene: Cldn8
Match Type: R21L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-CTCTG TTCTGTGTTCTGTTGGG  AGTTAAATCAAACGTATTTTC   ATGGAGCTTGGCATGTA GAAAA-3'
	3'-GAGAC AAGACACAAGACAACCC  TCAATTTAGTTTGCATAAAAG   TACCTCGAACCGTACAT CTTTT-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 011-028431745
Forward Primer: 5'-GCTCAGAACAGCTAATGTGAGTGC-3' Tm: 59.038
Reverse Primer: 5'-CAATGACTGTCCAGAACTGAGGC-3' Tm: 59.148
Expected PCR Product Length: 314
Expected CelI Cleavage Product Sizes: 177, 137
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr11:28431893-28431947

Rank: 85	Homology Score: 8	TALEN Score: 55.28
	Genomic Region: Intergenic	Closest Gene: Rgs10
Match Type: L22R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-AATTT TCTCTGCCCACCTCCGT  TGAAAGCATCCCCCCAAGGCTG  ATCACAAATACACTGAA GCTAA-3'
	3'-TTAAA AGAGACGGGTGGAGGCA  ACTTTCGTAGGGGGGTTCCGAC  TAGTGTTTATGTGACTT CGATT-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 001-187665749
Forward Primer: 5'-GGAGGGAAGGAGGGAAGGAA-3' Tm: 59.508
Reverse Primer: 5'-ACACCAGTCTTGCTGAGGGC-3' Tm: 59.508
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 132, 138
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr1:187665851-187665906

Rank: 86	Homology Score: 10	TALEN Score: 55.27
	Genomic Region: Intron	Closest Gene: Asip
Match Type: L22R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-AGGTT TCCCTGCGCTGCCTCAT  GATTAAGTTAACATTATTTTTA  GCAAGAACAACACAGAA GGGAT-3'
	3'-TCCAA AGGGACGCGACGGAGTA  CTAATTCAATTGTAATAAAAAT  CGTTCTTGTTGTGTCTT CCCTA-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 003-145456877
Forward Primer: 5'-GGCGACAGTTCCTCTGTTTTTGC-3' Tm: 59.148
Reverse Primer: 5'-CACAGTAGTGGCTGTAGGCATTTG-3' Tm: 59.038
Expected PCR Product Length: 375
Expected CelI Cleavage Product Sizes: 173, 202
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr3:145457020-145457075

Rank: 87	Homology Score: 5	TALEN Score: 55.23
	Genomic Region: Intergenic	Closest Gene: Snrk
Match Type: L24R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-GGCCT TTCCTTCACTGCTCCAC TCTTTGTCTCTGCATATCCTTTTG ACAGGAACAATTCTGGA TTAAT-3'
	3'-CCGGA AAGGAAGTGACGAGGTG AGAAACAGAGACGTATAGGAAAAC TGTCCTTGTTAAGACCT AATTA-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. Warning! The sequence of this region of the genome is poorly mapped. 
Primer Index: 00X-105203723
Forward Primer: 5'-CAGGTTCCTGTCAGCAAGTGTTTC-3' Tm: 59.038
Reverse Primer: 5'-GGGTTCAAATCCCCAAGGCTAC-3' Tm: 59.258
Expected PCR Product Length: 393
Expected CelI Cleavage Product Sizes: 133, 260
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chrX:105203825-105203882

Rank: 88	Homology Score: 10	TALEN Score: 55.23
	Genomic Region: Intron	Closest Gene: Cntn4
Match Type: L20R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-TTATC TACCTTCCCAGCCCCAT   TATGCATGGGTACAACATAA   ACCAACACAAAACAAAA GCAAA-3'
	3'-AATAG ATGGAAGGGTCGGGGTA   ATACGTACCCATGTTGTATT   TGGTTGTGTTTTGTTTT CGTTT-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 004-141883124
Forward Primer: 5'-AATCCGCAGTCTAAGCCAACAGAG-3' Tm: 59.038
Reverse Primer: 5'-TGCTAGGCAAGCACTCTACCAC-3' Tm: 59.258
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 134, 136
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr4:141883229-141883282

Rank: 89	Homology Score: 5	TALEN Score: 55.18
	Genomic Region: Intergenic	Closest Gene: Aldh8a1
Match Type: L23R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-TTTCT TTCAGTCTCTGCTCCAT TTTTGTCCCTGAATGTTCTTTAG  ACTAGAACAATTCTGAG TAAAC-3'
	3'-AAAGA AAGTCAGAGACGAGGTA AAAACAGGGACTTACAAGAAATC  TGATCTTGTTAAGACTC ATTTG-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
No Primers
Primer Index: No Primers
Forward Primer: 5'-No Primers-3' Tm: No Primers
Reverse Primer: 5'-No Primers-3' Tm: No Primers
Expected PCR Product Length: No Primers
Expected CelI Cleavage Product Sizes: No Primers, No Primers
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr1:16859003-16859059

Rank: 90	Homology Score: 8	TALEN Score: 55.17
	Genomic Region: Intergenic	Closest Gene: Amigo2
Match Type: R18L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-GGTTC TTCAGTGATGTACTGCA    TCAGGATGTGTAAGACAG    ATGAACCTGGGCTGGAG AGATG-3'
	3'-CCAAG AAGTCACTACATGACGT    AGTCCTACACATTCTGTC    TACTTGGACCCGACCTC TCTAC-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 007-135956125
Forward Primer: 5'-CACTCCTACGTAGGTATCTTGGG-3' Tm: 59.148
Reverse Primer: 5'-CTCACCAGAAGAGGGCATCAAATC-3' Tm: 59.038
Expected PCR Product Length: 280
Expected CelI Cleavage Product Sizes: 137, 143
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr7:135956234-135956285

Rank: 91	Homology Score: 5	TALEN Score: 55.16
	Genomic Region: Intergenic	Closest Gene: RGD1561230
Match Type: R12L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-ATTCT ATCAATGATGTTCTTGT       AGAGACTAATAC       ATGGCTCTTGTCATGAA AAGTT-3'
	3'-TAAGA TAGTTACTACAAGAACA       TCTCTGATTATG       TACCGAGAACAGTACTT TTCAA-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Warning! Primer pair was generated using suboptimal parameters. 
Primer Index: 00X-116419128
Forward Primer: 5'-CATGGTCATGACCTTACACAATATGTTACCC-3' Tm: 59.958
Reverse Primer: 5'-CCTCATGAGAAGTAGGCTCAAGGA-3' Tm: 59.038
Expected PCR Product Length: 370
Expected CelI Cleavage Product Sizes: 185, 185
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chrX:116419288-116419333

Rank: 92	Homology Score: 10	TALEN Score: 55.11
	Genomic Region: Intergenic	Closest Gene: Mir3589
Match Type: R22L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-CTTTG TAGACTGTGGTTCTGCT  GATTGTGGGATGACAGAAAGGA  ATGGAGATGGGCTAGAA GTCCA-3'
	3'-GAAAC ATCTGACACCAAGACGA  CTAACACCCTACTGTCTTTCCT  TACCTCTACCCGATCTT CAGGT-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 00X-146103263
Forward Primer: 5'-CTACACTCCTTTTTGTTGGGTGGG-3' Tm: 59.038
Reverse Primer: 5'-CTCTGCTGCAGAGCCTTTTTTCC-3' Tm: 59.148
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 136, 134
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chrX:146103369-146103424

Rank: 93	Homology Score: 5	TALEN Score: 55.08
	Genomic Region: Intergenic	Closest Gene: Pappa
Match Type: L24R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-TTCTC TACCTCCCCACCCCCAT TGCTATGGTTTTGCTCCCTGCATT TCCAGCACAATACAGTA GTGCG-3'
	3'-AAGAG ATGGAGGGGTGGGGGTA ACGATACCAAAACGAGGGACGTAA AGGTCGTGTTATGTCAT CACGC-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 005-082146486
Forward Primer: 5'-GCCAGAGGCCCTGGTCTTAT-3' Tm: 59.508
Reverse Primer: 5'-CTCCATTCCCTACTACAGAGAGTG-3' Tm: 59.038
Expected PCR Product Length: 278
Expected CelI Cleavage Product Sizes: 137, 141
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr5:82146592-82146649

Rank: 94	Homology Score: 5	TALEN Score: 55.08
	Genomic Region: Intergenic	Closest Gene: Fgf12
Match Type: R18L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-TATTC TTCAGTGTAATTCTTGC    CCTATAAATTTATGTTCT    CTGGAGGGTGGCAGAAA ATGTA-3'
	3'-ATAAG AAGTCACATTAAGAACG    GGATATTTAAATACAAGA    GACCTCCCACCGTCTTT TACAT-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 011-074482637
Forward Primer: 5'-ACAGACTTTGCAGAAGATCTAAGCCCTC-3' Tm: 60.298
Reverse Primer: 5'-GTCCTGGAAGTGACAGGTGTAC-3' Tm: 59.258
Expected PCR Product Length: 408
Expected CelI Cleavage Product Sizes: 131, 277
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr11:74482740-74482791

Rank: 95	Homology Score: 5	TALEN Score: 55.03
	Genomic Region: Intergenic	Closest Gene: RGD1561065
Match Type: R24L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-GGATA TGCATTGATGTACTGTT GCTGTGACAAAATTCTATGACCAA AGCGAGTTGGGGAGGAA AGGGT-3'
	3'-CCTAT ACGTAACTACATGACAA CGACACTGTTTTAAGATACTGGTT TCGCTCAACCCCTCCTT TCCCA-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 00X-040773744
Forward Primer: 5'-GGGTGAAACAGCATGTGGCATC-3' Tm: 59.258
Reverse Primer: 5'-GGTCCTATTAACTCCTACCTCCAG-3' Tm: 59.038
Expected PCR Product Length: 441
Expected CelI Cleavage Product Sizes: 310, 131
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chrX:40774023-40774080

Rank: 96	Homology Score: 5	TALEN Score: 55.02
	Genomic Region: Intron	Closest Gene: Scn3a
Match Type: L19R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-GGCAC TTCCCTCCCACCACCAC   CAATCATACACCTTACTCC    AACTGGACAAAACTGAA ACAAA-3'
	3'-CCGTG AAGGGAGGGTGGTGGTG   GTTAGTATGTGGAATGAGG    TTGACCTGTTTTGACTT TGTTT-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 003-048025715
Forward Primer: 5'-ACTGTCACACTGCTGTCACGG-3' Tm: 59.378
Reverse Primer: 5'-GACTGTCTTCGCAATAGGGTTATCTCATC-3' Tm: 60.178
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 131, 139
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr3:48025818-48025870

Rank: 97	Homology Score: 5	TALEN Score: 54.97
	Genomic Region: Intergenic	Closest Gene: Irak1bp1
Match Type: R22L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-GGCTC TCCAGTATTGAGCTGGA  GACTGGAGGTTCCTGAAAGAGC  ATGGTGCAGGTCAGGCA GTTTC-3'
	3'-CCGAG AGGTCATAACTCGACCT  CTGACCTCCAAGGACTTTCTCG  TACCACGTCCAGTCCGT CAAAG-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 008-087710971
Forward Primer: 5'-GTGGCTTATGGGTCGATGACCACA-3' Tm: 60.908
Reverse Primer: 5'-CTGCAGACTGCAAGGTTGTTCC-3' Tm: 59.258
Expected PCR Product Length: 315
Expected CelI Cleavage Product Sizes: 131, 184
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr8:87711072-87711127

Rank: 98	Homology Score: 5	TALEN Score: 54.95
	Genomic Region: Intergenic	Closest Gene: Tubgcp5
Match Type: R14L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-AAAAA GACAGTGTAGTACTGAT      TGAAGAGTTATGTT      ATGGAGATTGGCTTGAA GGTTT-3'
	3'-TTTTT CTGTCACATCATGACTA      ACTTCTCAATACAA      TACCTCTAACCGAACTT CCAAA-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 001-106482030
Forward Primer: 5'-GTGGTGGAACACCCTCTTAGAAGT-3' Tm: 59.038
Reverse Primer: 5'-CTCTATCTGCTGGTGGAATGAAGG-3' Tm: 59.038
Expected PCR Product Length: 271
Expected CelI Cleavage Product Sizes: 138, 133
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr1:106482142-106482189

Rank: 99	Homology Score: 5	TALEN Score: 54.93
	Genomic Region: Intergenic	Closest Gene: Gtf2e1
Match Type: L23R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-TTTCC TTCAGTCTCTGCTCCAT TTCTGTTCCCGCATTTCCTTTAG  ACAAGAACAATTCTGGA TCAAG-3'
	3'-AAAGG AAGTCAGAGACGAGGTA AAGACAAGGGCGTAAAGGAAATC  TGTTCTTGTTAAGACCT AGTTC-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 011-065225162
Forward Primer: 5'-TGCTAGCTTCCTGTCTGCAAGCAC-3' Tm: 60.908
Reverse Primer: 5'-ATGCCCAGTGATGGAAAGATGGAG-3' Tm: 59.038
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 139, 131
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr11:65225271-65225327

Rank: 100	Homology Score: 10	TALEN Score: 54.92
	Genomic Region: Exon	Closest Gene: Vom2r54
Match Type: R16L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-ATGAC CACAGTGGTGTTCAGTT     TATATCAGACTTGACA     AAGGAGCTGGACAAGAA TAAAA-3'
	3'-TACTG GTGTCACCACAAGTCAA     ATATAGTCTGAACTGT     TTCCTCGACCTGTTCTT ATTTT-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 007-014531040
Forward Primer: 5'-TCTCTGTATCAGGTGGCCCC-3' Tm: 59.508
Reverse Primer: 5'-CAAGAATCTCCCCATGATGAGGTG-3' Tm: 59.038
Expected PCR Product Length: 445
Expected CelI Cleavage Product Sizes: 132, 313
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr7:14531145-14531194

Plate format Oligo Order Form
Well
Name
Sequence
5' mods
Internal
Notes
Primer Plate #1
A1
006-138443970F
GGAATCGGCACATGCAGATCTTTG
Tesson_IgM_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
A2
006-138443970R
TCTGGTCCTCAGAGAGTCAGTC
Tesson_IgM_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
B1
020-016460972F
GTCAAGGACGAAGTCATCTGGAG
Tesson_IgM_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
B2
020-016460972R
GACAAGCTGACTGCTGCTAGAG
Tesson_IgM_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
C1
001-252428669F
GTTGTGTTTCAGGACAGCACTACC
Tesson_IgM_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
C2
001-252428669R
GTTGGAGAGGCCACATTCTTAACC
Tesson_IgM_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
D1
009-007190270F
AGTTGGGCTGGTAGAAATTGCTGG
Tesson_IgM_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
D2
009-007190270R
CTGAGTGAAACAAACGGCTCCGA
Tesson_IgM_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
E1
006-046986724F
CTTCTGGCTTAGTTTCCGTCTGTC
Tesson_IgM_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
E2
006-046986724R
CTCAGCTTGCCCAAGGGTTATAG
Tesson_IgM_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
F1
007-139879252F
AAAGGCTCATTAGACACGCTCAGG
Tesson_IgM_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
F2
007-139879252R
CACGTCTCTGCAGACCCCAT
Tesson_IgM_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
G1
005-161756246F
CTCTAGGGGCTAAGTATCTCAGCT
Tesson_IgM_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
G2
005-161756246R
GGTGGCAAAATCTAAACCCTGTGG
Tesson_IgM_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
H1
009-019047855F
GTGGCTTTGGTCCTCATCTGTC
Tesson_IgM_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
H2
009-019047855R
CCTCTTTCGGAGATAGGCATGTTC
Tesson_IgM_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
A3
002-204001007F
AATTTATGGGCTCTGTGCCGTGC
Tesson_IgM_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
A4
002-204001007R
CCCAGTGCTAAGTTGAGTGTGGC
Tesson_IgM_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
B3
019-057896612F
CCCAGTGCTGGGGTTACAAACA
Tesson_IgM_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
B4
019-057896612R
TCTTAGCCAGGAATGAGGCTGC
Tesson_IgM_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
C3
014-008802083F
TGCCAGGCTCCTGTCTGCAT
Tesson_IgM_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
C4
014-008802083R
GCTACCAGGACTCAATGGGG
Tesson_IgM_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
D3
008-080224511F
GGGTAGCGACTCTGCAGACA
Tesson_IgM_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
D4
008-080224511R
GTGACTGTGGTGAGCTGGAG
Tesson_IgM_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
E3
005-113632907F
GGAGGAATCTCTAGAAAGTCCCAG
Tesson_IgM_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
E4
005-113632907R
GGCTCCTATCTGCAGGTCCA
Tesson_IgM_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
F3
003-151465311F
CACACACACACACACACACACAC
Tesson_IgM_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
F4
003-151465311R
CACCAGTGACAACAGCACTTGAG
Tesson_IgM_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
G3
003-100952790F
GCCATGAAGAAGACATGCAGGAG
Tesson_IgM_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
G4
003-100952790R
GGATCACTTGAAATGTGGAGCTTCCTAC
Tesson_IgM_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
H3
009-065106893F
CCTCTGTTGGATGTGGAGTTGG
Tesson_IgM_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
H4
009-065106893R
CGCACACCTATGGACATCTGAG
Tesson_IgM_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
A5
011-031478392F
GATCTGATCCCCTTTTCTGGTGTG
Tesson_IgM_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
A6
011-031478392R
CTTCTTTAGCACGTCGCCCC
Tesson_IgM_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
B5
009-004673112F
AATCCACCGAGCCTAGTAACTGC
Tesson_IgM_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
B6
009-004673112R
TACACTGCGCTCACACAGCAAG
Tesson_IgM_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
C5
014-082098705F
AGCCAAGGAAGAAACCTGTGCAGC
Tesson_IgM_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
C6
014-082098705R
CTGTGGATGGCTAGAGTGTACTCA
Tesson_IgM_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
D5
006-075907048F
GGTGTCCTCATTCACCTCTGACAA
Tesson_IgM_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
D6
006-075907048R
GTGTTTGGAACATCCTCTGCGAC
Tesson_IgM_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
E5
00X-027254372F
CCATTATCGCTAGCCTAGCTTACG
Tesson_IgM_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
E6
00X-027254372R
GACTGGGAATAAGTGGTGTGGTAC
Tesson_IgM_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
F5
010-013862379F
CATAGGTGGGGCCTTATCAGAG
Tesson_IgM_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
F6
010-013862379R
GGTCCCTAGTCAAGGACATTGTCT
Tesson_IgM_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
G5
001-189623849F
TGCATTCCCACCCCCATACTG
Tesson_IgM_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
G6
001-189623849R
CTGAGCACATTCAGGCATCTGG
Tesson_IgM_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
H5
001-140972716F
GGGACCACCTCAGCTAAGGA
Tesson_IgM_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
H6
001-140972716R
GTGGGGTTGTTGGTTTTCAGCTC
Tesson_IgM_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
A7
00X-131557987F
TTTTGGATGTCAGCTATAGGGTTGGAGAAAATCTT
Tesson_IgM_Ranking-TALENv2.0	Warning! Primer pair was generated using suboptimal parameters. 
A8
00X-131557987R
CAACAAATGATGCTAATCTAACTGGATGTCTACATG
Tesson_IgM_Ranking-TALENv2.0	Warning! Primer pair was generated using suboptimal parameters. 
B7
011-011914436F
GACACAGTACTCAGAGGATCCAAG
Tesson_IgM_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
B8
011-011914436R
GCTTGGCTTCTAGGACCAGAC
Tesson_IgM_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
C7
005-123636672F
CTCTGAGTGACACGAATTAGTGGG
Tesson_IgM_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
C8
005-123636672R
GGAGTGTGTCACTAAGGCTGG
Tesson_IgM_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
D7
004-003843423F
GGTGACCAATACCAACCATCAGG
Tesson_IgM_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
D8
004-003843423R
CCTCCTTTCAGGAAGGCTCTTTCT
Tesson_IgM_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
E7
00X-016509931F
CTACCAATGTCTATGGAGTGACCG
Tesson_IgM_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
E8
00X-016509931R
GCCTAGAGTATCCCAAATGTGCAC
Tesson_IgM_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
F7
005-036123844F
GGCTGTAGACACTCTTCAGCTTG
Tesson_IgM_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
F8
005-036123844R
GTGACCTCTCTGCAGACAGC
Tesson_IgM_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
G7
003-107514838F
GTTGGCTTAAGGCTGCCAACTTC
Tesson_IgM_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
G8
003-107514838R
GTGTGTTGCCTCCTTTTCTACACG
Tesson_IgM_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
H7
004-054569528F
CTGTGAGCAAGCCAACAGATAGC
Tesson_IgM_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
H8
004-054569528R
CCTTGGAGTTGCTACAGGCTTTG
Tesson_IgM_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
A9
00X-144712285F
CCAAGCGAGACAGAGATCGG
Tesson_IgM_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
A10
00X-144712285R
GGTACTTGGCCCTCAGAATCG
Tesson_IgM_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
B9
020-043706993F
GCAGAAATGGATAGTTTGTGGGGG
Tesson_IgM_Ranking-TALENv2.0	Primer pair generated using optimal parameters. Warning! The sequence of this region of the genome is poorly mapped. 
B10
020-043706993R
CCGCCAAGCCCTTTTTAAGTACTG
Tesson_IgM_Ranking-TALENv2.0	Primer pair generated using optimal parameters. Warning! The sequence of this region of the genome is poorly mapped. 
C9
004-100499585F
GGCATAGCCTCTCAGGAAACAG
Tesson_IgM_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
C10
004-100499585R
CTTCCAGGACAGAACAGGAAAGAC
Tesson_IgM_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
D9
003-060396362F
CCACTGGGAGTGTATTCGGTATG
Tesson_IgM_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
D10
003-060396362R
GTAAACAGGCCTCTACAGAACGG
Tesson_IgM_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
E9
002-159432579F
GCTAAATCCCCAACCCTGCAG
Tesson_IgM_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
E10
002-159432579R
GTGTTTACTTGCTCAAACACCCGG
Tesson_IgM_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
F9
004-135905706F
GACAACGACTGCGTTCTATTTGGG
Tesson_IgM_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
F10
004-135905706R
TCACGAGCCCTGGTTATTGTTTGG
Tesson_IgM_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
G9
001-256112389F
CCTGGATAAGTCAGTAAAGGGTGG
Tesson_IgM_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
G10
001-256112389R
CCTTGGTGGGACCTGATACAGT
Tesson_IgM_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
H9
012-033556886F
TCTGTCTGCAAGCTCCAGGG
Tesson_IgM_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
H10
012-033556886R
AACTCTGGCCTCCTTGGTCC
Tesson_IgM_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
A11
006-090557385F
GTCCCCACAGGCATATGATCG
Tesson_IgM_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
A12
006-090557385R
GTGCTTCGGGCAGTAGAGCT
Tesson_IgM_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
B11
007-067524783F
TGATCAGAGCCTGCCCCTTC
Tesson_IgM_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
B12
007-067524783R
GACAGGGTGCTTCCTCACAATAC
Tesson_IgM_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
C11
0Un-021787610F
CCTGGGATGTGGTGTAAGTTGG
Tesson_IgM_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
C12
0Un-021787610R
GAGGAGCCAAATCTGGACCATTAG
Tesson_IgM_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
D11
dom-003376644F
CCTGGGATGTGGTGTAAGTTGG
Tesson_IgM_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
D12
dom-003376644R
GAGGAGCCAAATCTGGACCATTAG
Tesson_IgM_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
E11
0Un-038608458F
CCTGGGATGTGGTGTAAGTTGG
Tesson_IgM_Ranking-TALENv2.0	Primer pair generated using optimal parameters. Warning! The sequence of this region of the genome is poorly mapped. 
E12
0Un-038608458R
GAGACAACATACCCTGCTCTCAC
Tesson_IgM_Ranking-TALENv2.0	Primer pair generated using optimal parameters. Warning! The sequence of this region of the genome is poorly mapped. 
F11
0Un-051334472F
CCTGGGATGTGGTGTAAGTTGG
Tesson_IgM_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
F12
0Un-051334472R
GAGGAGCCAAATCTGGACCATTAG
Tesson_IgM_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
G11
0Un-061148009F
CCTGGGATGTGGTGTAAGTTGG
Tesson_IgM_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
G12
0Un-061148009R
GAGACAACATACCCTGCTCTCAC
Tesson_IgM_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
H11
001-127719260F
CCAACATTCTCCCAAGAGATTCTGGAAAC
Tesson_IgM_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
H12
001-127719260R
GTTCCAGGTCAACCAGGACTAC
Tesson_IgM_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
Primer Plate #2
A1
008-068387105F
CCGACTAGCTTCTCCCATGAC
Tesson_IgM_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
A2
008-068387105R
TTACTGTCGGTGTCACAGTGCC
Tesson_IgM_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
B1
018-068645313F
CTGGGAGCCTGGAAATTTGTCTG
Tesson_IgM_Ranking-TALENv2.0	Warning! Primer pair was generated using suboptimal parameters. 
B2
018-068645313R
GGACACAAGCATATATTATTCTTTGGATGTGCTC
Tesson_IgM_Ranking-TALENv2.0	Warning! Primer pair was generated using suboptimal parameters. 
C1
010-070982895F
CAGGGTGTGTCTTTCCCAAGATAC
Tesson_IgM_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
C2
010-070982895R
GGGTCTCAGGAAACGTTGTGTTG
Tesson_IgM_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
D1
009-052013154F
GAGGCTGAGCTTGGCTTGCTT
Tesson_IgM_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
D2
009-052013154R
GCAAGGTCACATAGTGTCACTTGG
Tesson_IgM_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
E1
003-046502425F
GCCAAGGCTAAAGCAGTTTGGAAG
Tesson_IgM_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
E2
003-046502425R
GGAGGCACATTCTTAGGCTGCT
Tesson_IgM_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
F1
001-026132578F
CATTCAGATACTCTGAAGCGAAACATGTTAAGG
Tesson_IgM_Ranking-TALENv2.0	Warning! Primer pair was generated using suboptimal parameters. 
F2
001-026132578R
CCCCTGACCAATACTTTGCAAACG
Tesson_IgM_Ranking-TALENv2.0	Warning! Primer pair was generated using suboptimal parameters. 
G1
004-126653383F
CCATGTGACCTTTGGACTACATGC
Tesson_IgM_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
G2
004-126653383R
GAGAGCCACTGACTACCATAACAC
Tesson_IgM_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
H1
014-039438747F
GCTCCATAAACTGGGCCTGAGA
Tesson_IgM_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
H2
014-039438747R
GCTAGCCTCTACCTCTTCCTG
Tesson_IgM_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
A3
001-242536006F
TGGAGGTTGGGAGAGTAGAGG
Tesson_IgM_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
A4
001-242536006R
TGAAGGAGGGAACACTCAGCAG
Tesson_IgM_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
B3
005-170773963F
CCAAGGTTGCTGCCAGTTCAGCT
Tesson_IgM_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
B4
005-170773963R
AGGAGCTGGAGAGCCAAGTG
Tesson_IgM_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
C3
013-002441765F
CTGTCTCTGTCTCTCTTAGTTCCC
Tesson_IgM_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
C4
013-002441765R
CCCTTTTTGCCTTTCCATGCAGTG
Tesson_IgM_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
D3
003-124119953F
CAGTAGCTGCCTTTGACCAGGA
Tesson_IgM_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
D4
003-124119953R
CAAACCAGACAATGGCTTCTGCTC
Tesson_IgM_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
E3
002-175409656F
CATCGGGCAGGGTTTCAAAGC
Tesson_IgM_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
E4
002-175409656R
GCCCAAGCTGACATTTGAGAGAC
Tesson_IgM_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
F3
004-174994043F
GTGCGGCTCTGTCCTCACAT
Tesson_IgM_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
F4
004-174994043R
GATTAAATTGCATGCAGCTGCACTAACTCC
Tesson_IgM_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
G3
008-060163371F
TGGTCAATGTCCTTGGGGCTG
Tesson_IgM_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
G4
008-060163371R
CGAGTCTTTCTAGCCAGTCAAGTG
Tesson_IgM_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
H3
008-060289566F
TGGTCAATGTCCTTGGGGCTG
Tesson_IgM_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
H4
008-060289566R
CGAGTCTTTCTAGCCAGTCAAGTG
Tesson_IgM_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
A5
012-043552977F
CACCCACTTCATGTTGGAAACGC
Tesson_IgM_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
A6
012-043552977R
TTCCAAACCCTCTGCCCTGC
Tesson_IgM_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
B5
002-106965669F
ATAGTGGCCAGTGCCTGGAC
Tesson_IgM_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
B6
002-106965669R
CCAGGAGAAAAGGCCATCTTCCA
Tesson_IgM_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
C5
005-063999032F
CAGGAGCAAGACAAGAGTGTCTAC
Tesson_IgM_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
C6
005-063999032R
CGGTTAGTGTGGGATGTCTTTCTC
Tesson_IgM_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
D5
0Un-075352159F
CTTGAGAGACTGTGGTGAATGAGG
Tesson_IgM_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
D6
0Un-075352159R
GGAGGAAAAAGGGGACTGTGTTCA
Tesson_IgM_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
E5
004-092024652F
CTTGAGAGACTGTGGTGAATGAGG
Tesson_IgM_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
E6
004-092024652R
GGAGGAAAAAGGGGACTGTGTTCA
Tesson_IgM_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
F5
003-128243778F
CAACCACACTGCAGACCAGG
Tesson_IgM_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
F6
003-128243778R
GCCATGGATCTGATATTGGTGGCT
Tesson_IgM_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
G5
012-015478690F
CCACTCAAGACAAGCAGAAAGGAC
Tesson_IgM_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
G6
012-015478690R
CAAAACAGCAGCAGCAAAAGCTGG
Tesson_IgM_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
H5
006-079162173F
ACACAGAAGGCTCTCCAGAGAC
Tesson_IgM_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
H6
006-079162173R
ACTTTGATCTCACTTCAGGTCCCC
Tesson_IgM_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
A7
007-112677493F
TAGGGCAGAGCTGATGGACC
Tesson_IgM_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
A8
007-112677493R
CGAGTCTCTTCTTGACTGTTTCCC
Tesson_IgM_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
B7
001-138061468F
CCGTGAAGCAGGTCACAAAGC
Tesson_IgM_Ranking-TALENv2.0	Primer pair generated using optimal parameters. Warning! The sequence of this region of the genome is poorly mapped. 
B8
001-138061468R
CAGCAAGGGAAGTGCTACTTGATG
Tesson_IgM_Ranking-TALENv2.0	Primer pair generated using optimal parameters. Warning! The sequence of this region of the genome is poorly mapped. 
C7
00X-010426535F
GTTCTCTGCATCCATGCCATGTG
Tesson_IgM_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
C8
00X-010426535R
CCTCAGCATCAGTTACTGCTTCC
Tesson_IgM_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
D7
010-053902466F
CCCATTACCACCCTCCCCTA
Tesson_IgM_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
D8
010-053902466R
AGGGAGACAAATGAGGGTTTTGTCCTTC
Tesson_IgM_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
E7
015-087079625F
CAATCACTCCCTGCAATCCCC
Tesson_IgM_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
E8
015-087079625R
TGACGCCTCCACTGTCCTAG
Tesson_IgM_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
F7
001-172645535F
CTCCTGTTTGCAAGCACATCTTGG
Tesson_IgM_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
F8
001-172645535R
CTGAAATGTCCAACAGTGGGGC
Tesson_IgM_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
G7
017-041218534F
CCAGGCCATGATTATTCTGCAGG
Tesson_IgM_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
G8
017-041218534R
TCCCAAGTCACCTGCAAGCC
Tesson_IgM_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
H7
008-044521883F
CAGAGGACCTGTGAGAGACAGT
Tesson_IgM_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
H8
008-044521883R
GAAGCAAATGTGAGAGGAAAAGCCTTGATG
Tesson_IgM_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
A9
005-049320556F
CTGTGGTGAAGGTGTTACTGTGTC
Tesson_IgM_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
A10
005-049320556R
GAGAAGGCTGGACCGAATAGAAG
Tesson_IgM_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
B9
012-031851371F
GGGTGCCTGACTGCTATATTTCC
Tesson_IgM_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
B10
012-031851371R
TTGCCTGCCACAACCATGCATC
Tesson_IgM_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
C9
002-139129605F
CCCATGTGCAGAAGGAGTTGTAC
Tesson_IgM_Ranking-TALENv2.0	Primer pair generated using optimal parameters. Warning! The sequence of this region of the genome is poorly mapped. 
C10
002-139129605R
GTTCCAGGTCAGTCAGGGATATAC
Tesson_IgM_Ranking-TALENv2.0	Primer pair generated using optimal parameters. Warning! The sequence of this region of the genome is poorly mapped. 
D9
011-028431745F
GCTCAGAACAGCTAATGTGAGTGC
Tesson_IgM_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
D10
011-028431745R
CAATGACTGTCCAGAACTGAGGC
Tesson_IgM_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
E9
001-187665749F
GGAGGGAAGGAGGGAAGGAA
Tesson_IgM_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
E10
001-187665749R
ACACCAGTCTTGCTGAGGGC
Tesson_IgM_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
F9
003-145456877F
GGCGACAGTTCCTCTGTTTTTGC
Tesson_IgM_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
F10
003-145456877R
CACAGTAGTGGCTGTAGGCATTTG
Tesson_IgM_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
G9
00X-105203723F
CAGGTTCCTGTCAGCAAGTGTTTC
Tesson_IgM_Ranking-TALENv2.0	Primer pair generated using optimal parameters. Warning! The sequence of this region of the genome is poorly mapped. 
G10
00X-105203723R
GGGTTCAAATCCCCAAGGCTAC
Tesson_IgM_Ranking-TALENv2.0	Primer pair generated using optimal parameters. Warning! The sequence of this region of the genome is poorly mapped. 
H9
004-141883124F
AATCCGCAGTCTAAGCCAACAGAG
Tesson_IgM_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
H10
004-141883124R
TGCTAGGCAAGCACTCTACCAC
Tesson_IgM_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
A11
No PrimersF
No Primers
Tesson_IgM_Ranking-TALENv2.0	No Primers
A12
No PrimersR
No Primers
Tesson_IgM_Ranking-TALENv2.0	No Primers
B11
007-135956125F
CACTCCTACGTAGGTATCTTGGG
Tesson_IgM_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
B12
007-135956125R
CTCACCAGAAGAGGGCATCAAATC
Tesson_IgM_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
C11
00X-116419128F
CATGGTCATGACCTTACACAATATGTTACCC
Tesson_IgM_Ranking-TALENv2.0	Warning! Primer pair was generated using suboptimal parameters. 
C12
00X-116419128R
CCTCATGAGAAGTAGGCTCAAGGA
Tesson_IgM_Ranking-TALENv2.0	Warning! Primer pair was generated using suboptimal parameters. 
D11
00X-146103263F
CTACACTCCTTTTTGTTGGGTGGG
Tesson_IgM_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
D12
00X-146103263R
CTCTGCTGCAGAGCCTTTTTTCC
Tesson_IgM_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
E11
005-082146486F
GCCAGAGGCCCTGGTCTTAT
Tesson_IgM_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
E12
005-082146486R
CTCCATTCCCTACTACAGAGAGTG
Tesson_IgM_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
F11
011-074482637F
ACAGACTTTGCAGAAGATCTAAGCCCTC
Tesson_IgM_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
F12
011-074482637R
GTCCTGGAAGTGACAGGTGTAC
Tesson_IgM_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
G11
00X-040773744F
GGGTGAAACAGCATGTGGCATC
Tesson_IgM_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
G12
00X-040773744R
GGTCCTATTAACTCCTACCTCCAG
Tesson_IgM_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
H11
003-048025715F
ACTGTCACACTGCTGTCACGG
Tesson_IgM_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
H12
003-048025715R
GACTGTCTTCGCAATAGGGTTATCTCATC
Tesson_IgM_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
Primer Plate #3
A1
008-087710971F
GTGGCTTATGGGTCGATGACCACA
Tesson_IgM_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
A2
008-087710971R
CTGCAGACTGCAAGGTTGTTCC
Tesson_IgM_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
B1
001-106482030F
GTGGTGGAACACCCTCTTAGAAGT
Tesson_IgM_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
B2
001-106482030R
CTCTATCTGCTGGTGGAATGAAGG
Tesson_IgM_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
C1
011-065225162F
TGCTAGCTTCCTGTCTGCAAGCAC
Tesson_IgM_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
C2
011-065225162R
ATGCCCAGTGATGGAAAGATGGAG
Tesson_IgM_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
D1
007-014531040F
TCTCTGTATCAGGTGGCCCC
Tesson_IgM_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
D2
007-014531040R
CAAGAATCTCCCCATGATGAGGTG
Tesson_IgM_Ranking-TALENv2.0	Primer pair generated using optimal parameters.