PROGNOS Summary for Tesson_IgM_Ranking-RVDs_5TC

The rn4 genome was searched for the TALEN pair targeting:
5'- TTCCTGCCCAGCTCCAT NN..NN ACCAGAACAACACTGAA -3'

Using these RVDs:
Left:  01NG02HD03HD04NG05NN06HD07HD08HD09NI10NN11HD12NG13HD14HD15NI16NG
Right: 01NG02HD03NI04NN05NG06NN07NG08NG09NN10NG11NG12HD13NG14NN15NN16NG

Up to 5 mismatches were allowed in each nuclease half-site.

Allowed spacing distances were: 12 13 14 15 16 17 18 19 20 21 22 23 24

Only heterodimers of the nuclease pair were included in the output

1973 total sites were located
Mismatch Types
Genomic Regions
  0 and 0: 1
  3 and 3: 1
  2 and 5: 2
  3 and 4: 10
  3 and 5: 55
  4 and 4: 22
  4 and 5: 408
  5 and 5: 1474
  Exons: 35
  Promoters: 8
  Introns: 485
  Intergenic: 1445


The top 100 sites are ranked below according to a weighting of the RVD base preferences. All sites listed contain flanking 5' pyrimidines.
For the full output, click here.

This output will be deleted from the server one week after it is generated.  Please save your results.

Primer pairs were designed using the 400 bp of sequence on either side of the cleavage site to melt close to 60 °C

For easy visualization of cleavage on gels:
The smallest fragment will be longer than 130 bp, which is also the minimum separation distance between uncleaved and cleaved products.
The two cleavage products will be separated by a minimum size of 0 bp
To optimize assay conditions, the amplicons have a minimum length of 260 bp, a maximum length of 450 bp, and an optimal length of 270 bp.
Rank: 1	Homology Score: 72	RVD Match Score: 2	Left Half-Site RVD Score: 3.404	Right Half-Site RVD Score: 2.361
	Genomic Region: Intergenic	Closest Gene: Adam6	Caution! This site scores very closely to the intended nuclease target. The potential for cleavage at this site is of great concern.
Match Type: R17L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-ATGAC TTCAGTGTTGTTCTGGT    AGTTCCAGGAGAAGGAA     ATGGAGCTGGGCAGGAA GTCCC-3'
	3'-TACTG AAGTCACAACAAGACCA    TCAAGGTCCTCTTCCTT     TACCTCGACCCGTCCTT CAGGG-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 006-138443970
Forward Primer: 5'-GGAATCGGCACATGCAGATCTTTG-3' Tm: 59.038
Reverse Primer: 5'-TCTGGTCCTCAGAGAGTCAGTC-3' Tm: 59.258
Expected PCR Product Length: 325
Expected CelI Cleavage Product Sizes: 194, 131
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr6:138444137-138444187

Rank: 2	Homology Score: 10	RVD Match Score: 2.614	Left Half-Site RVD Score: 5.634	Right Half-Site RVD Score: 3.899
	Genomic Region: Intron	Closest Gene: Sorcs1
Match Type: L23R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-TGATG CTGCTGCTCAACTCCCT TGCTAACAACTGAATACATCTGT  ATCAGAACAACAGTGAG CAAAA-3'
	3'-ACTAC GACGACGAGTTGAGGGA ACGATTGTTGACTTATGTAGACA  TAGTCTTGTTGTCACTC GTTTT-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 001-256112389
Forward Primer: 5'-CCTGGATAAGTCAGTAAAGGGTGG-3' Tm: 59.038
Reverse Primer: 5'-CCTTGGTGGGACCTGATACAGT-3' Tm: 59.258
Expected PCR Product Length: 353
Expected CelI Cleavage Product Sizes: 204, 149
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr1:256112563-256112619

Rank: 3	Homology Score: 5	RVD Match Score: 2.622	Left Half-Site RVD Score: 4.296	Right Half-Site RVD Score: 5.521
	Genomic Region: Intergenic	Closest Gene: Snhg11
Match Type: L13R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-TCCGG CTCCTCCCCATCTCCAC      ACACCTGAGAATA       ACTAGAATAACAAAGAG CGGCA-3'
	3'-AGGCC GAGGAGGGGTAGAGGTG      TGTGGACTCTTAT       TGATCTTATTGTTTCTC GCCGT-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 003-149194978
Forward Primer: 5'-CTTGTTAGGTGAAGCAGGATGGC-3' Tm: 59.148
Reverse Primer: 5'-TCACACCGGTGCTGAAGGAAC-3' Tm: 59.378
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 136, 134
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr3:149195089-149195135

Rank: 4	Homology Score: 13	RVD Match Score: 2.639	Left Half-Site RVD Score: 4.477	Right Half-Site RVD Score: 5.426
	Genomic Region: Intergenic	Closest Gene: Tssc1
Match Type: L17R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-GGGGG CTCCTGCCCAGATCCTT    CTCTGCCAGGCTGCCCC     TCCAGCACAATATTGAA TCTCA-3'
	3'-CCCCC GAGGACGGGTCTAGGAA    GAGACGGTCCGACGGGG     AGGTCGTGTTATAACTT AGAGT-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 006-046986724
Forward Primer: 5'-CTTCTGGCTTAGTTTCCGTCTGTC-3' Tm: 59.038
Reverse Primer: 5'-CTCAGCTTGCCCAAGGGTTATAG-3' Tm: 59.148
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 130, 140
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr6:46986827-46986877

Rank: 5	Homology Score: 18	RVD Match Score: 2.668	Left Half-Site RVD Score: 5.677	Right Half-Site RVD Score: 4.477
	Genomic Region: Intron	Closest Gene: Pdcd11
Match Type: R16L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-TTGGA TGCAGACTTGTTCTGGT     CCTGTTAGCAGACCTC     AGGGATCTGGGGAGGAA TTAGC-3'
	3'-AACCT ACGTCTGAACAAGACCA     GGACAATCGTCTGGAG     TCCCTAGACCCCTCCTT AATCG-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 001-252428669
Forward Primer: 5'-GTTGTGTTTCAGGACAGCACTACC-3' Tm: 59.038
Reverse Primer: 5'-GTTGGAGAGGCCACATTCTTAACC-3' Tm: 59.038
Expected PCR Product Length: 308
Expected CelI Cleavage Product Sizes: 162, 146
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr1:252428804-252428853

Rank: 6	Homology Score: 13	RVD Match Score: 2.67	Left Half-Site RVD Score: 6.001	Right Half-Site RVD Score: 4.258
	Genomic Region: Intron	Closest Gene: Acvrl1
Match Type: R21L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-AAGGC TTCATTGAGGTTCTGGT  CTTCATGATCACAGAAGCGGC   CTGGAGTTGGGAAGGAG GAAGA-3'
	3'-TTCCG AAGTAACTCCAAGACCA  GAAGTACTAGTGTCTTCGCCG   GACCTCAACCCTTCCTC CTTCT-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 007-139879252
Forward Primer: 5'-AAAGGCTCATTAGACACGCTCAGG-3' Tm: 59.038
Reverse Primer: 5'-CACGTCTCTGCAGACCCCAT-3' Tm: 59.508
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 134, 136
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr7:139879357-139879411

Rank: 7	Homology Score: 5	RVD Match Score: 2.683	Left Half-Site RVD Score: 5.043	Right Half-Site RVD Score: 5.086
	Genomic Region: Intron	Closest Gene: Top1
Match Type: L21R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-TGGAC TTCCTGCACCACTCAAT  GGCTGTGATTATCAGCACAAG   ACATGAACAATATTGAG CCTGT-3'
	3'-ACCTG AAGGACGTGGTGAGTTA  CCGACACTAATAGTCGTGTTC   TGTACTTGTTATAACTC GGACA-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 003-151465311
Forward Primer: 5'-CACACACACACACACACACACAC-3' Tm: 59.148
Reverse Primer: 5'-CACCAGTGACAACAGCACTTGAG-3' Tm: 59.148
Expected PCR Product Length: 330
Expected CelI Cleavage Product Sizes: 186, 144
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr3:151465468-151465522

Rank: 8	Homology Score: 10	RVD Match Score: 2.705	Left Half-Site RVD Score: 3.639	Right Half-Site RVD Score: 7.301
	Genomic Region: Intergenic	Closest Gene: Mir532
Match Type: L21R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-TTTTT TTCCTACCCACCCCCAT  GCCTCCCCAGCCTTGGATGAA   ACAGGAACAAAAGTCAA AATTT-3'
	3'-AAAAA AAGGATGGGTGGGGGTA  CGGAGGGGTCGGAACCTACTT   TGTCCTTGTTTTCAGTT TTAAA-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 00X-027254372
Forward Primer: 5'-CCATTATCGCTAGCCTAGCTTACG-3' Tm: 59.038
Reverse Primer: 5'-GACTGGGAATAAGTGGTGTGGTAC-3' Tm: 59.038
Expected PCR Product Length: 301
Expected CelI Cleavage Product Sizes: 167, 134
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chrX:27254510-27254564

Rank: 9	Homology Score: 13	RVD Match Score: 2.739	Left Half-Site RVD Score: 4.34	Right Half-Site RVD Score: 6.522
	Genomic Region: Intergenic	Closest Gene: Fgfr2
Match Type: L22R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-TTCTG CTCCACCCCAGCTCCAA  AGGAAATGGCTGCCCATAAATC  ACCAGAAAAACACCAAA GTCCC-3'
	3'-AAGAC GAGGTGGGGTCGAGGTT  TCCTTTACCGACGGGTATTTAG  TGGTCTTTTTGTGGTTT CAGGG-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 001-189623849
Forward Primer: 5'-TGCATTCCCACCCCCATACTG-3' Tm: 59.378
Reverse Primer: 5'-CTGAGCACATTCAGGCATCTGG-3' Tm: 59.258
Expected PCR Product Length: 297
Expected CelI Cleavage Product Sizes: 131, 166
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr1:189623950-189624005

Rank: 10	Homology Score: 2	RVD Match Score: 2.74	Left Half-Site RVD Score: 6.222	Right Half-Site RVD Score: 4.551
	Genomic Region: Intergenic	Closest Gene: Usp15
Match Type: R14L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-TTGGA CTCTGTACTGTTCTTGT      TCATTAAGAACCCT      AGGGACTTGGGTAGGAG AAAGG-3'
	3'-AACCT GAGACATGACAAGAACA      AGTAATTCTTGGGA      TCCCTGAACCCATCCTC TTTCC-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 007-062964516
Forward Primer: 5'-TCAAGGCGCAGTCAACCTCC-3' Tm: 59.508
Reverse Primer: 5'-CTGTCAAGGTTGAACAAGGGACTG-3' Tm: 59.038
Expected PCR Product Length: 353
Expected CelI Cleavage Product Sizes: 156, 197
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr7:62964646-62964693

Rank: 11	Homology Score: 17	RVD Match Score: 2.748	Left Half-Site RVD Score: 3.946	Right Half-Site RVD Score: 7.218
	Genomic Region: Intergenic	Closest Gene: Lrfn2
Match Type: L24R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-TCCCC TTCCTGCCCAGCTCCCA TCCCCACCCCCTTCTGAGAACTAA ACAGGAAGCACACGGAA CCTGC-3'
	3'-AGGGG AAGGACGGGTCGAGGGT AGGGGTGGGGGAAGACTCTTGATT TGTCCTTCGTGTGCCTT GGACG-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 009-007190270
Forward Primer: 5'-AGTTGGGCTGGTAGAAATTGCTGG-3' Tm: 59.038
Reverse Primer: 5'-CTGAGTGAAACAAACGGCTCCGA-3' Tm: 59.148
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 133, 137
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr9:7190372-7190429

Rank: 12	Homology Score: 5	RVD Match Score: 2.751	Left Half-Site RVD Score: 4.644	Right Half-Site RVD Score: 5.921
	Genomic Region: Intergenic	Closest Gene: Ccnc
Match Type: R15L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-TCATA CTCAAGGTTGTTCTGAT     GCAAACAACCTCCTC      ATTGTTCTGGGTAGTAA ACAGA-3'
	3'-AGTAT GAGTTCCAACAAGACTA     CGTTTGTTGGAGGAG      TAACAAGACCCATCATT TGTCT-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 005-036123844
Forward Primer: 5'-GGCTGTAGACACTCTTCAGCTTG-3' Tm: 59.148
Reverse Primer: 5'-GTGACCTCTCTGCAGACAGC-3' Tm: 59.508
Expected PCR Product Length: 346
Expected CelI Cleavage Product Sizes: 140, 206
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr5:36123958-36124006

Rank: 13	Homology Score: 8	RVD Match Score: 2.759	Left Half-Site RVD Score: 5.576	Right Half-Site RVD Score: 5.095
	Genomic Region: Intergenic	Closest Gene: Rps29
Match Type: L16R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-GCACA TTCCTCCACATTTCCAT     CTCGAGGAAAGATTGG     ATCAGATCAAGAGTGAA ACTCA-3'
	3'-CGTGT AAGGAGGTGTAAAGGTA     GAGCTCCTTTCTAACC     TAGTCTAGTTCTCACTT TGAGT-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 006-090557385
Forward Primer: 5'-GTCCCCACAGGCATATGATCG-3' Tm: 59.378
Reverse Primer: 5'-GTGCTTCGGGCAGTAGAGCT-3' Tm: 59.508
Expected PCR Product Length: 348
Expected CelI Cleavage Product Sizes: 217, 131
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr6:90557575-90557624

Rank: 14	Homology Score: 5	RVD Match Score: 2.76	Left Half-Site RVD Score: 5.563	Right Half-Site RVD Score: 5.116
	Genomic Region: Intergenic	Closest Gene: Arl5b
Match Type: L16R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-TAATT CTCACTCCCAGCACCAT     ACCCTAAATTTCTAAG     AGCAGAACAATACAGAG ACTTT-3'
	3'-ATTAA GAGTGAGGGTCGTGGTA     TGGGATTTAAAGATTC     TCGTCTTGTTATGTCTC TGAAA-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 017-089324125
Forward Primer: 5'-GGATTTGGGAGAGGAAGGATGAG-3' Tm: 59.148
Reverse Primer: 5'-CATAGTGACACACCCTGAAGTTGC-3' Tm: 59.038
Expected PCR Product Length: 426
Expected CelI Cleavage Product Sizes: 294, 132
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr17:89324392-89324441

Rank: 15	Homology Score: 13	RVD Match Score: 2.76	Left Half-Site RVD Score: 5.076	Right Half-Site RVD Score: 5.706
	Genomic Region: Intergenic	Closest Gene: Wdfy3
Match Type: L23R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-TTTCT TTCAGTCCCAGCTCCAT TTTTGTCCCTGCATTGCCTTTAG  ACAGGAACAACTCTGAG TTGCA-3'
	3'-AAAGA AAGTCAGGGTCGAGGTA AAAACAGGGACGTAACGGAAATC  TGTCCTTGTTGAGACTC AACGT-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 014-008802083
Forward Primer: 5'-TGCCAGGCTCCTGTCTGCAT-3' Tm: 59.508
Reverse Primer: 5'-GCTACCAGGACTCAATGGGG-3' Tm: 59.508
Expected PCR Product Length: 299
Expected CelI Cleavage Product Sizes: 139, 160
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr14:8802192-8802248

Rank: 16	Homology Score: 5	RVD Match Score: 2.771	Left Half-Site RVD Score: 7.418	Right Half-Site RVD Score: 3.963
	Genomic Region: Intergenic	Closest Gene: Mllt1
Match Type: R23L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-TACAG CACAGTGGGGTTCTGGG GGGATGGGTGGGCCGGCAGGAGG  GTGGAGTTGGGAAGGAG AGCAC-3'
	3'-ATGTC GTGTCACCCCAAGACCC CCCTACCCACCCGGCCGTCCTCC  CACCTCAACCCTTCCTC TCGTG-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 0Un-024874250
Forward Primer: 5'-GCCTTCAATTCAGTGAGGAATGGG-3' Tm: 59.038
Reverse Primer: 5'-TACTCAAGTGTGTGCCATGCACC-3' Tm: 59.148
Expected PCR Product Length: 286
Expected CelI Cleavage Product Sizes: 138, 148
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chrUn:24874358-24874414

Rank: 17	Homology Score: 5	RVD Match Score: 2.775	Left Half-Site RVD Score: 6.076	Right Half-Site RVD Score: 4.891
	Genomic Region: Intergenic	Closest Gene: Fyn
Match Type: R14L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-GGAGA TCCAGTTTTCTTCTTGT      CAGGGGTGGGTGGG      ATGGGGTGGGGTAGGTA GGAGA-3'
	3'-CCTCT AGGTCAAAAGAAGAACA      GTCCCCACCCACCC      TACCCCACCCCATCCAT CCTCT-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. Warning! The sequence of this region of the genome is poorly mapped. 
Primer Index: 020-043706993
Forward Primer: 5'-GCAGAAATGGATAGTTTGTGGGGG-3' Tm: 59.038
Reverse Primer: 5'-CCGCCAAGCCCTTTTTAAGTACTG-3' Tm: 59.038
Expected PCR Product Length: 378
Expected CelI Cleavage Product Sizes: 248, 130
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr20:43707215-43707262

Rank: 18	Homology Score: 5	RVD Match Score: 2.786	Left Half-Site RVD Score: 5.664	Right Half-Site RVD Score: 5.212
	Genomic Region: Intron	Closest Gene: Prickle2
Match Type: L13R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-CCTGT TTCCTTCCCAAATCCCC      TGACAAGACATAG       AGCAGAAGAACATTTAA AATTA-3'
	3'-GGACA AAGGAAGGGTTTAGGGG      ACTGTTCTGTATC       TCGTCTTCTTGTAAATT TTAAT-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 004-126653383
Forward Primer: 5'-CCATGTGACCTTTGGACTACATGC-3' Tm: 59.038
Reverse Primer: 5'-GAGAGCCACTGACTACCATAACAC-3' Tm: 59.038
Expected PCR Product Length: 327
Expected CelI Cleavage Product Sizes: 145, 182
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr4:126653503-126653549

Rank: 19	Homology Score: 5	RVD Match Score: 2.79	Left Half-Site RVD Score: 5.049	Right Half-Site RVD Score: 5.841
	Genomic Region: Intron	Closest Gene: Il1rapl1
Match Type: R17L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-TGTGA CCCAGAATTGTTCTGGT    CTGAAAGAACTACAGGG     ATGGAAATGGAGAGGAG CCTGA-3'
	3'-ACACT GGGTCTTAACAAGACCA    GACTTTCTTGATGTCCC     TACCTTTACCTCTCCTC GGACT-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 00X-074124866
Forward Primer: 5'-AATGCCCTACAGTGGGGAGAG-3' Tm: 59.378
Reverse Primer: 5'-CAAAGGACAGTCATGATAGGTCCC-3' Tm: 59.038
Expected PCR Product Length: 282
Expected CelI Cleavage Product Sizes: 130, 152
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chrX:74124969-74125019

Rank: 20	Homology Score: 10	RVD Match Score: 2.794	Left Half-Site RVD Score: 4.36	Right Half-Site RVD Score: 7.012
	Genomic Region: Intron	Closest Gene: Rnf4
Match Type: L19R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-GTAGA TCCCTCCCCAGCTCCCT   TGTCAGGACCTAGCCAGGT    ACATGATCAACATTCAA GAAAA-3'
	3'-CATCT AGGGAGGGGTCGAGGGA   ACAGTCCTGGATCGGTCCA    TGTACTAGTTGTAAGTT CTTTT-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 014-082098705
Forward Primer: 5'-AGCCAAGGAAGAAACCTGTGCAGC-3' Tm: 60.908
Reverse Primer: 5'-CTGTGGATGGCTAGAGTGTACTCA-3' Tm: 59.038
Expected PCR Product Length: 300
Expected CelI Cleavage Product Sizes: 130, 170
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr14:82098807-82098859

Rank: 21	Homology Score: 10	RVD Match Score: 2.794	Left Half-Site RVD Score: 7.385	Right Half-Site RVD Score: 4.124
	Genomic Region: Intergenic	Closest Gene: Pard3
Match Type: R12L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-TTTGC TTTTTTATTGTTCTGGA       AGTTAAGTTTGA       ATGGGGCTTGGTAGGAA ATGAT-3'
	3'-AAACG AAAAAATAACAAGACCT       TCAATTCAAACT       TACCCCGAACCATCCTT TACTA-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 019-057896612
Forward Primer: 5'-CCCAGTGCTGGGGTTACAAACA-3' Tm: 59.258
Reverse Primer: 5'-TCTTAGCCAGGAATGAGGCTGC-3' Tm: 59.258
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 138, 132
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr19:57896725-57896770

Rank: 22	Homology Score: 10	RVD Match Score: 2.802	Left Half-Site RVD Score: 6.686	Right Half-Site RVD Score: 4.635
	Genomic Region: Intergenic	Closest Gene: Pde4b
Match Type: R15L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-TTTCG TCCATTATTGTCATGGT     GTCATGCAAGGTGAC      ATGGTGCTGGGGAGGCA GCTGA-3'
	3'-AAAGC AGGTAATAACAGTACCA     CAGTACGTTCCACTG      TACCACGACCCCTCCGT CGACT-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 005-123636672
Forward Primer: 5'-CTCTGAGTGACACGAATTAGTGGG-3' Tm: 59.038
Reverse Primer: 5'-GGAGTGTGTCACTAAGGCTGG-3' Tm: 59.378
Expected PCR Product Length: 350
Expected CelI Cleavage Product Sizes: 218, 132
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr5:123636864-123636912

Rank: 23	Homology Score: 5	RVD Match Score: 2.803	Left Half-Site RVD Score: 5.862	Right Half-Site RVD Score: 5.251
	Genomic Region: Intergenic	Closest Gene: Tasp1
Match Type: R14L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-TGTCT TTCAGTTTTGTTGTACT      GAGAGAGAAAGAGA      ATGAAGTTGGGTGGGTA GGAGG-3'
	3'-ACAGA AAGTCAAAACAACATGA      CTCTCTCTTTCTCT      TACTTCAACCCACCCAT CCTCC-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 003-128243778
Forward Primer: 5'-CAACCACACTGCAGACCAGG-3' Tm: 59.508
Reverse Primer: 5'-GCCATGGATCTGATATTGGTGGCT-3' Tm: 59.038
Expected PCR Product Length: 308
Expected CelI Cleavage Product Sizes: 131, 177
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr3:128243883-128243930

Rank: 24	Homology Score: 13	RVD Match Score: 2.805	Left Half-Site RVD Score: 6.508	Right Half-Site RVD Score: 4.782
	Genomic Region: Intergenic	Closest Gene: Atpbd4
Match Type: R15L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-CATAA TTCAATGTTGTTTGGGC     TTTTTTTTTTTTTTT      TTGGAGCTGGGGACGAA CCCAG-3'
	3'-GTATT AAGTTACAACAAACCCG     AAAAAAAAAAAAAAA      AACCTCGACCCCTGCTT GGGTC-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 003-100952790
Forward Primer: 5'-GCCATGAAGAAGACATGCAGGAG-3' Tm: 59.148
Reverse Primer: 5'-GGATCACTTGAAATGTGGAGCTTCCTAC-3' Tm: 60.298
Expected PCR Product Length: 405
Expected CelI Cleavage Product Sizes: 218, 187
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr3:100952982-100953030

Rank: 25	Homology Score: 5	RVD Match Score: 2.809	Left Half-Site RVD Score: 5.089	Right Half-Site RVD Score: 6.127
	Genomic Region: Intergenic	Closest Gene: Bnc1
Match Type: L21R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-CGGCT CCCCTGTCCAGCTCCAC  TTGCAGACTCTCTTTGCTCCC   ACCAGAACTCTGCTGAG TGACC-3'
	3'-GCCGA GGGGACAGGTCGAGGTG  AACGTCTGAGAGAAACGAGGG   TGGTCTTGAGACGACTC ACTGG-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 001-138291107
Forward Primer: 5'-TATCTATGTGGCAGGCTGGGTAC-3' Tm: 59.148
Reverse Primer: 5'-GTGCAGTGTCATACCCGGGTT-3' Tm: 59.378
Expected PCR Product Length: 279
Expected CelI Cleavage Product Sizes: 130, 149
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr1:138291208-138291262

Rank: 26	Homology Score: 5	RVD Match Score: 2.816	Left Half-Site RVD Score: 3.971	Right Half-Site RVD Score: 7.859
	Genomic Region: Intergenic	Closest Gene: Caln1
Match Type: L18R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-TCTCT CTCCTCCCCACCCCCAT    GCACAGCATGTATGCACA    CCCAGAAAAAAAATAAA TACAT-3'
	3'-AGAGA GAGGAGGGGTGGGGGTA    CGTGTCGTACATACGTGT    GGGTCTTTTTTTTATTT ATGTA-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 012-027291771
Forward Primer: 5'-TCCTAAGGGTGGCCTTTTGACCTC-3' Tm: 60.908
Reverse Primer: 5'-CAGCCTCAGCTCACAGAGATC-3' Tm: 59.378
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 134, 136
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr12:27291877-27291928

Rank: 27	Homology Score: 17	RVD Match Score: 2.822	Left Half-Site RVD Score: 5.219	Right Half-Site RVD Score: 5.929
	Genomic Region: Intergenic	Closest Gene: RGD1559903
Match Type: R16L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-TTCCA TGCAGTGTTGTTCTGTT     GTCAGTAATGGATGCC     AGAGAGTTGGGATGGAA TGAGA-3'
	3'-AAGGT ACGTCACAACAAGACAA     CAGTCATTACCTACGG     TCTCTCAACCCTACCTT ACTCT-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 020-016460972
Forward Primer: 5'-GTCAAGGACGAAGTCATCTGGAG-3' Tm: 59.148
Reverse Primer: 5'-GACAAGCTGACTGCTGCTAGAG-3' Tm: 59.258
Expected PCR Product Length: 340
Expected CelI Cleavage Product Sizes: 144, 196
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr20:16461089-16461138

Rank: 28	Homology Score: 5	RVD Match Score: 2.825	Left Half-Site RVD Score: 5.484	Right Half-Site RVD Score: 5.722
	Genomic Region: Intergenic	Closest Gene: Gnaz
Match Type: R16L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-TTAGA CTCTGTATTGCTCTGGT     AAAACATATGACCAAA     AGTAAGTTGGGGAGGAA GGGGT-3'
	3'-AATCT GAGACATAACGAGACCA     TTTTGTATACTGGTTT     TCATTCAACCCCTCCTT CCCCA-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 020-014621051
Forward Primer: 5'-GCCTGTGGTTGGTACATGTACCT-3' Tm: 59.148
Reverse Primer: 5'-GAGCCGTGATTCTCCATGGATTC-3' Tm: 59.148
Expected PCR Product Length: 366
Expected CelI Cleavage Product Sizes: 236, 130
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr20:14621260-14621309

Rank: 29	Homology Score: 2	RVD Match Score: 2.829	Left Half-Site RVD Score: 5.5	Right Half-Site RVD Score: 5.737
	Genomic Region: Intergenic	Closest Gene: LOC500827
Match Type: R13L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-TCTGA TTCAATAATCTTCTGTT      TTATAAAGTCCTT       ATGGATCTTGGGAAGAG GTAGT-3'
	3'-AGACT AAGTTATTAGAAGACAA      AATATTTCAGGAA       TACCTAGAACCCTTCTC CATCA-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 007-038298476
Forward Primer: 5'-AAAAGCATGAGCTCCTCAGGCTGG-3' Tm: 60.908
Reverse Primer: 5'-GAAGAGGAAACTGTGGTCAGGTTG-3' Tm: 59.038
Expected PCR Product Length: 370
Expected CelI Cleavage Product Sizes: 130, 240
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr7:38298581-38298627

Rank: 30	Homology Score: 13	RVD Match Score: 2.829	Left Half-Site RVD Score: 4.456	Right Half-Site RVD Score: 7.218
	Genomic Region: Intron	Closest Gene: Sars
Match Type: L22R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-GTAAG TACCTCCCCAGCTCAAT  TCCACTCACACAACCACAGATA  ACCAGGCCAAAACAGAA GTACC-3'
	3'-CATTC ATGGAGGGGTCGAGTTA  AGGTGAGTGTGTTGGTGTCTAT  TGGTCCGGTTTTGTCTT CATGG-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 002-204001007
Forward Primer: 5'-AATTTATGGGCTCTGTGCCGTGC-3' Tm: 59.148
Reverse Primer: 5'-CCCAGTGCTAAGTTGAGTGTGGC-3' Tm: 61.088
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 135, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr2:204001112-204001167

Rank: 31	Homology Score: 5	RVD Match Score: 2.831	Left Half-Site RVD Score: 5.503	Right Half-Site RVD Score: 5.796
	Genomic Region: Intergenic	Closest Gene: Csmd1
Match Type: L15R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-AGTCA TTCCTGCTCACCCCCAC     ACTGACAGAAAGAAA      ACCTGAAGAGGACTGAG TAAAG-3'
	3'-TCAGT AAGGACGAGTGGGGGTG     TGACTGTCTTTCTTT      TGGACTTCTCCTGACTC ATTTC-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 016-077159880
Forward Primer: 5'-CTGTGGAGTTTGTGCAGGAATGCT-3' Tm: 59.038
Reverse Primer: 5'-GGGGCAGTGTGTGATCAAAAGG-3' Tm: 59.258
Expected PCR Product Length: 360
Expected CelI Cleavage Product Sizes: 170, 190
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr16:77160024-77160072

Rank: 32	Homology Score: 2	RVD Match Score: 2.834	Left Half-Site RVD Score: 6.029	Right Half-Site RVD Score: 5.34
	Genomic Region: Intron	Closest Gene: Prex1
Match Type: R14L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-GAACC TTAAGTACTATCCTGGT      AGAAGCGGTTTGGG      AGGGGTTTGGGGAGGAA GTCCA-3'
	3'-CTTGG AATTCATGATAGGACCA      TCTTCGCCAAACCC      TCCCCAAACCCCTCCTT CAGGT-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 003-157836620
Forward Primer: 5'-GGCAAAGCTGCCTGTCACCA-3' Tm: 59.508
Reverse Primer: 5'-GCTTCAGGCACAGCTGGATC-3' Tm: 59.508
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 136, 134
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr3:157836730-157836777

Rank: 33	Homology Score: 13	RVD Match Score: 2.836	Left Half-Site RVD Score: 6.116	Right Half-Site RVD Score: 5.124
	Genomic Region: Intron	Closest Gene: Ppm1l
Match Type: L12R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-ACTCT TTCCTCCCAAGCTGCTT       TTGGGCGGTTTT       ACCACAATAACAGTGAA GCAAA-3'
	3'-TGAGA AAGGAGGGTTCGACGAA       AACCCGCCAAAA       TGGTGTTATTGTCACTT CGTTT-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 002-159432579
Forward Primer: 5'-GCTAAATCCCCAACCCTGCAG-3' Tm: 59.378
Reverse Primer: 5'-GTGTTTACTTGCTCAAACACCCGG-3' Tm: 59.038
Expected PCR Product Length: 284
Expected CelI Cleavage Product Sizes: 154, 130
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr2:159432708-159432753

Rank: 34	Homology Score: 5	RVD Match Score: 2.848	Left Half-Site RVD Score: 7.493	Right Half-Site RVD Score: 4.397
	Genomic Region: Intergenic	Closest Gene: Myo5b
Match Type: R12L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-CCCTG TGCAGTGTGGATCTTCT       TTGGTGTAGATA       AAGGAGTTGGGAAGGAG CTGGA-3'
	3'-GGGAC ACGTCACACCTAGAAGA       AACCACATCTAT       TTCCTCAACCCTTCCTC GACCT-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 018-071340665
Forward Primer: 5'-CCCCCATCCCCTTTTACAGTTC-3' Tm: 59.258
Reverse Primer: 5'-GTAGAGGTCCCTCCTTGAGATTC-3' Tm: 59.148
Expected PCR Product Length: 301
Expected CelI Cleavage Product Sizes: 148, 153
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr18:71340788-71340833

Rank: 35	Homology Score: 5	RVD Match Score: 2.849	Left Half-Site RVD Score: 5.944	Right Half-Site RVD Score: 5.428
	Genomic Region: Intergenic	Closest Gene: Lypd1
Match Type: L21R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-AGCTT TTCCTTCACAGCATCAT  GTGTCCATGACCACATAGGCA   AGCAGAACAAGAGTGTG CTTTG-3'
	3'-TCGAA AAGGAAGTGTCGTAGTA  CACAGGTACTGGTGTATCCGT   TCGTCTTGTTCTCACAC GAAAC-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 013-038906135
Forward Primer: 5'-GCCCCACAGCTATCTCTTTTTGGA-3' Tm: 59.038
Reverse Primer: 5'-CTGGAGACAGCTTTCAAATCACCC-3' Tm: 59.038
Expected PCR Product Length: 436
Expected CelI Cleavage Product Sizes: 298, 138
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr13:38906404-38906458

Rank: 36	Homology Score: 10	RVD Match Score: 2.849	Left Half-Site RVD Score: 7.669	Right Half-Site RVD Score: 4.296
	Genomic Region: Intergenic	Closest Gene: Mctp2
Match Type: R14L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-AATCA TTCAGTGAATTTGTTGT      GTCACATCTGCCCC      ATGGAGGTGGGAGGGAA GAATA-3'
	3'-TTAGT AAGTCACTTAAACAACA      CAGTGTAGACGGGG      TACCTCCACCCTCCCTT CTTAT-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 001-127719260
Forward Primer: 5'-CCAACATTCTCCCAAGAGATTCTGGAAAC-3' Tm: 60.178
Reverse Primer: 5'-GTTCCAGGTCAACCAGGACTAC-3' Tm: 59.258
Expected PCR Product Length: 368
Expected CelI Cleavage Product Sizes: 135, 233
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr1:127719369-127719416

Rank: 37	Homology Score: 2	RVD Match Score: 2.852	Left Half-Site RVD Score: 6.266	Right Half-Site RVD Score: 5.282
	Genomic Region: Intergenic	Closest Gene: Arid1a
Match Type: R23L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-TCCTA CTCTGTGTTGCTCAGAT TGGTTTTCGAACTCTTAATCTTA  CTGGGTTTGGGTAGGAA TTTCA-3'
	3'-AGGAT GAGACACAACGAGTCTA ACCAAAAGCTTGAGAATTAGAAT  GACCCAAACCCATCCTT AAAGT-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 005-151331548
Forward Primer: 5'-CTGACCCAGGGCAAGTCATTTAC-3' Tm: 59.148
Reverse Primer: 5'-GGACCTCCAACCAGATGAACTAAC-3' Tm: 59.038
Expected PCR Product Length: 368
Expected CelI Cleavage Product Sizes: 173, 195
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr5:151331691-151331747

Rank: 38	Homology Score: 10	RVD Match Score: 2.853	Left Half-Site RVD Score: 5.066	Right Half-Site RVD Score: 6.57
	Genomic Region: Intron	Closest Gene: Ralgapa1
Match Type: L13R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-TCTCC TTCCTTTCCAGCTCCCT      TCTTTCTTTCATT       ACTAGATATGCACTGAA AGCTG-3'
	3'-AGAGG AAGGAAAGGTCGAGGGA      AGAAAGAAAGTAA       TGATCTATACGTGACTT TCGAC-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 006-075907048
Forward Primer: 5'-GGTGTCCTCATTCACCTCTGACAA-3' Tm: 59.038
Reverse Primer: 5'-GTGTTTGGAACATCCTCTGCGAC-3' Tm: 59.148
Expected PCR Product Length: 363
Expected CelI Cleavage Product Sizes: 189, 174
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr6:75907212-75907258

Rank: 39	Homology Score: 10	RVD Match Score: 2.854	Left Half-Site RVD Score: 6.541	Right Half-Site RVD Score: 5.089
	Genomic Region: Intergenic	Closest Gene: Zfand6
Match Type: R15L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-AGGAG TTCAGTCATGTTCAAGG     GCACTTCTTGTGTAA      ATGGGGCTGGACAGGGA GGAGT-3'
	3'-TCCTC AAGTCAGTACAAGTTCC     CGTGAAGAACACATT      TACCCCGACCTGTCCCT CCTCA-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 001-140972716
Forward Primer: 5'-GGGACCACCTCAGCTAAGGA-3' Tm: 59.508
Reverse Primer: 5'-GTGGGGTTGTTGGTTTTCAGCTC-3' Tm: 59.148
Expected PCR Product Length: 368
Expected CelI Cleavage Product Sizes: 173, 195
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr1:140972863-140972911

Rank: 40	Homology Score: 5	RVD Match Score: 2.855	Left Half-Site RVD Score: 5.365	Right Half-Site RVD Score: 6.049
	Genomic Region: Exon	Closest Gene: LOC686841
Match Type: R18L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-TGGCC CTCCATGTTGTTCGGGT    GGGTCCTTGTCAAAGTGG    TTGTACCTGGGCAGGGG GGTAA-3'
	3'-ACCGG GAGGTACAACAAGCCCA    CCCAGGAACAGTTTCACC    AACATGGACCCGTCCCC CCATT-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
No Primers
Primer Index: No Primers
Forward Primer: 5'-No Primers-3' Tm: No Primers
Reverse Primer: 5'-No Primers-3' Tm: No Primers
Expected PCR Product Length: No Primers
Expected CelI Cleavage Product Sizes: No Primers, No Primers
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr7:116335856-116335907

Rank: 41	Homology Score: 5	RVD Match Score: 2.861	Left Half-Site RVD Score: 4.831	Right Half-Site RVD Score: 6.975
	Genomic Region: Intergenic	Closest Gene: Slc39a11
Match Type: L13R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-CATGG CTCCTATCCAGGTCCAT      GGTTTCTGACGAA       ACTGGCACAACAGAGAA CAGGT-3'
	3'-GTACC GAGGATAGGTCCAGGTA      CCAAAGACTGCTT       TGACCGTGTTGTCTCTT GTCCA-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 010-102159896
Forward Primer: 5'-GGAACCACCCATTTCCTTGGTGT-3' Tm: 59.148
Reverse Primer: 5'-GTGTGTCTGTCTGTCTGTCTGTC-3' Tm: 59.148
Expected PCR Product Length: 291
Expected CelI Cleavage Product Sizes: 146, 145
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr10:102160017-102160063

Rank: 42	Homology Score: 2	RVD Match Score: 2.863	Left Half-Site RVD Score: 5.673	Right Half-Site RVD Score: 5.877
	Genomic Region: Intergenic	Closest Gene: Prep
Match Type: L20R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-CGCGC CTCCTGCACGCCCCCAT   CAGAGTCTGTAACTGGGGTA   ACGTGAGTAAGACTGAA AAGCT-3'
	3'-GCGCG GAGGACGTGCGGGGGTA   GTCTCAGACATTGACCCCAT   TGCACTCATTCTGACTT TTCGA-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 020-049001036
Forward Primer: 5'-GGGGGAGGAGAGTAGTAAATTCG-3' Tm: 59.148
Reverse Primer: 5'-TTCGCGACTTGCCGACGAGA-3' Tm: 59.508
Expected PCR Product Length: 283
Expected CelI Cleavage Product Sizes: 153, 130
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr20:49001160-49001213

Rank: 43	Homology Score: 2	RVD Match Score: 2.864	Left Half-Site RVD Score: 5.503	Right Half-Site RVD Score: 6.096
	Genomic Region: Intergenic	Closest Gene: Trim42
Match Type: L15R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-CTTAG CCCCTCTCCAGCACCAT     GTCTGCATGCAAGTC      ACTAGAAGAACATATAA TGATA-3'
	3'-GAATC GGGGAGAGGTCGTGGTA     CAGACGTACGTTCAG      TGATCTTCTTGTATATT ACTAT-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: dom-000644656
Forward Primer: 5'-GGAGAGAGAAAGGGCGATAGG-3' Tm: 59.378
Reverse Primer: 5'-GCACCTGAGATAAAAGACCCTCTG-3' Tm: 59.038
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 137, 133
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr8_random:644767-644815

Rank: 44	Homology Score: 10	RVD Match Score: 2.865	Left Half-Site RVD Score: 6.046	Right Half-Site RVD Score: 5.448
	Genomic Region: Intergenic	Closest Gene: Fundc1
Match Type: L19R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-AGACC TTCCTAACCCTTTCCAT   TTGCTTAAATTAACAGAGA    AACAGAACAATACTCAA AATTG-3'
	3'-TCTGG AAGGATTGGGAAAGGTA   AACGAATTTAATTGTCTCT    TTGTCTTGTTATGAGTT TTAAC-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 00X-016509931
Forward Primer: 5'-CTACCAATGTCTATGGAGTGACCG-3' Tm: 59.038
Reverse Primer: 5'-GCCTAGAGTATCCCAAATGTGCAC-3' Tm: 59.038
Expected PCR Product Length: 365
Expected CelI Cleavage Product Sizes: 130, 235
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chrX:16510033-16510085

Rank: 45	Homology Score: 5	RVD Match Score: 2.867	Left Half-Site RVD Score: 6.642	Right Half-Site RVD Score: 5.105
	Genomic Region: Intergenic	Closest Gene: Ppp4r2
Match Type: R15L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-TAAAT TTAAATTTTTTTCTGGA     GGGTGATCACAGGGG      TTGGAGTGGGGCAGGCA CAGCA-3'
	3'-ATTTA AATTTAAAAAAAGACCT     CCCACTAGTGTCCCC      AACCTCACCCCGTCCGT GTCGT-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 004-135905706
Forward Primer: 5'-GACAACGACTGCGTTCTATTTGGG-3' Tm: 59.038
Reverse Primer: 5'-TCACGAGCCCTGGTTATTGTTTGG-3' Tm: 59.038
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 134, 136
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr4:135905814-135905862

Rank: 46	Homology Score: 10	RVD Match Score: 2.867	Left Half-Site RVD Score: 7.279	Right Half-Site RVD Score: 4.664
	Genomic Region: Exon	Closest Gene: Lrp1
Match Type: R20L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-GCTGT TTCAATCTTGGGGTGGT   TCCCCCAGTCTGACCAGTAC   ATGGTGCTGGGAAGGGA GAGAT-3'
	3'-CGACA AAGTTAGAACCCCACCA   AGGGGGTCAGACTGGTCATG   TACCACGACCCTTCCCT CTCTA-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 007-067524783
Forward Primer: 5'-TGATCAGAGCCTGCCCCTTC-3' Tm: 59.508
Reverse Primer: 5'-GACAGGGTGCTTCCTCACAATAC-3' Tm: 59.148
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 138, 132
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr7:67524892-67524945

Rank: 47	Homology Score: 10	RVD Match Score: 2.869	Left Half-Site RVD Score: 5.089	Right Half-Site RVD Score: 6.686
	Genomic Region: Intron	Closest Gene: Tsc2
Match Type: L18R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-TTAGC TACCTGTCCAGCACCAT    GTCTGCCTGGTGCCTCCC    ACCATGACAATAATGGA CTAAA-3'
	3'-AATCG ATGGACAGGTCGTGGTA    CAGACGGACCACGGAGGG    TGGTACTGTTATTACCT GATTT-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 010-013862379
Forward Primer: 5'-CATAGGTGGGGCCTTATCAGAG-3' Tm: 59.258
Reverse Primer: 5'-GGTCCCTAGTCAAGGACATTGTCT-3' Tm: 59.038
Expected PCR Product Length: 273
Expected CelI Cleavage Product Sizes: 131, 142
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr10:13862482-13862533

Rank: 48	Homology Score: 2	RVD Match Score: 2.869	Left Half-Site RVD Score: 5.086	Right Half-Site RVD Score: 6.409
	Genomic Region: Intergenic	Closest Gene: Mis18bp1
Match Type: R14L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-AGAGT CTCATAGTTATTCTGAT      CCTCATCTCATGTG      ATCGAGTTAGAGAGGAA AGTCT-3'
	3'-TCTCA GAGTATCAATAAGACTA      GGAGTAGAGTACAC      TAGCTCAATCTCTCCTT TCAGA-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 006-086619661
Forward Primer: 5'-GGCTTCAGGAAAAGGTTGATTCCC-3' Tm: 59.038
Reverse Primer: 5'-CGTGAAGGAGTCTCGTGTCAAC-3' Tm: 59.258
Expected PCR Product Length: 354
Expected CelI Cleavage Product Sizes: 217, 137
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr6:86619852-86619899

Rank: 49	Homology Score: 2	RVD Match Score: 2.869	Left Half-Site RVD Score: 6.589	Right Half-Site RVD Score: 5.163
	Genomic Region: Intergenic	Closest Gene: Plcl1
Match Type: R19L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-GGAAT TTCAGGGTCATTTTGAT   TTGCATTTCCCTTTGGGCC    TGGGAGGGGGGCAGGAG AGAAG-3'
	3'-CCTTA AAGTCCCAGTAAAACTA   AACGTAAAGGGAAACCCGG    ACCCTCCCCCCGTCCTC TCTTC-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 009-054998586
Forward Primer: 5'-CTCTCTCTCACGGGGTCTAG-3' Tm: 59.508
Reverse Primer: 5'-AGTTCTGAGAGTCTCTCACCTCC-3' Tm: 59.148
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 135, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr9:54998693-54998745

Rank: 50	Homology Score: 2	RVD Match Score: 2.871	Left Half-Site RVD Score: 6.657	Right Half-Site RVD Score: 5.122
	Genomic Region: Intergenic	Closest Gene: Hace1
Match Type: R22L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-CCCTG TAAAATGTGCTTCTGGT  TATTGCAATGTAAGACTTAGGA  GTGGAGGTGGGCTGGGG TTTGC-3'
	3'-GGGAC ATTTTACACGAAGACCA  ATAACGTTACATTCTGAATCCT  CACCTCCACCCGACCCC AAACG-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 020-051519222
Forward Primer: 5'-GTGTTCCTGAACTCTAGTAGAGGC-3' Tm: 59.038
Reverse Primer: 5'-TAGCAGAACTCCTGCCCGTG-3' Tm: 59.508
Expected PCR Product Length: 403
Expected CelI Cleavage Product Sizes: 273, 130
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr20:51519465-51519520

Rank: 51	Homology Score: 8	RVD Match Score: 2.876	Left Half-Site RVD Score: 6.164	Right Half-Site RVD Score: 5.532
	Genomic Region: Intergenic	Closest Gene: Amigo2
Match Type: R18L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-GGTTC TTCAGTGATGTACTGCA    TCAGGATGTGTAAGACAG    ATGAACCTGGGCTGGAG AGATG-3'
	3'-CCAAG AAGTCACTACATGACGT    AGTCCTACACATTCTGTC    TACTTGGACCCGACCTC TCTAC-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 007-135956125
Forward Primer: 5'-CACTCCTACGTAGGTATCTTGGG-3' Tm: 59.148
Reverse Primer: 5'-CTCACCAGAAGAGGGCATCAAATC-3' Tm: 59.038
Expected PCR Product Length: 280
Expected CelI Cleavage Product Sizes: 137, 143
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr7:135956234-135956285

Rank: 52	Homology Score: 10	RVD Match Score: 2.879	Left Half-Site RVD Score: 4.838	Right Half-Site RVD Score: 7.136
	Genomic Region: Intergenic	Closest Gene: Fam174a
Match Type: L24R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-CTCCC TTCTTCCCCAGATCCAT TTCACCTCCATTTCCTCTTCATAA ACCAGCAGACCACTAAG AGACA-3'
	3'-GAGGG AAGAAGGGGTCTAGGTA AAGTGGAGGTAAAGGAGAAGTATT TGGTCGTCTGGTGATTC TCTGT-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 009-094128198
Forward Primer: 5'-GTGACACTGGGAAAATCAGTAGGC-3' Tm: 59.038
Reverse Primer: 5'-GGTGTGTCTCTGGCTCTTTTGC-3' Tm: 59.258
Expected PCR Product Length: 378
Expected CelI Cleavage Product Sizes: 247, 131
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr9:94128414-94128471

Rank: 53	Homology Score: 8	RVD Match Score: 2.879	Left Half-Site RVD Score: 6.557	Right Half-Site RVD Score: 5.014
	Genomic Region: Intron	Closest Gene: Col27a1
Match Type: L24R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-CTGGC TTCCTGCCTATCTCCTG CCCTATGCCAGTCACAGGGTAGCT AGGTGAACAACACTGAG CTTTG-3'
	3'-GACCG AAGGACGGATAGAGGAC GGGATACGGTCAGTGTCCCATCGA TCCACTTGTTGTGACTC GAAAC-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 005-080218256
Forward Primer: 5'-TCTCCCTAAGAGAAGGGGTGG-3' Tm: 59.378
Reverse Primer: 5'-GTCACTGCTTACACTGAATGACCC-3' Tm: 59.038
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 134, 136
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr5:80218359-80218416

Rank: 54	Homology Score: 8	RVD Match Score: 2.882	Left Half-Site RVD Score: 5.188	Right Half-Site RVD Score: 6.672
	Genomic Region: Intergenic	Closest Gene: Lamp5
Match Type: L24R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-ATACC TTCCACCCCACCTCCTT CCCCTAGATTTCAAGAAGGGGGGA AGCAGAACAAAACCTAA CCTCG-3'
	3'-TATGG AAGGTGGGGTGGAGGAA GGGGATCTAAAGTTCTTCCCCCCT TCGTCTTGTTTTGGATT GGAGC-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 003-124119953
Forward Primer: 5'-CAGTAGCTGCCTTTGACCAGGA-3' Tm: 59.258
Reverse Primer: 5'-CAAACCAGACAATGGCTTCTGCTC-3' Tm: 59.038
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 140, 130
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr3:124120062-124120119

Rank: 55	Homology Score: 10	RVD Match Score: 2.882	Left Half-Site RVD Score: 7.727	Right Half-Site RVD Score: 4.47
	Genomic Region: Intergenic	Closest Gene: LOC679825
Match Type: R16L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-TGAGG TTTACTTTTGCTGTGGT     AAAACATCATGACCAA     AGGGACTTGGGCAGGAA AGAGT-3'
	3'-ACTCC AAATGAAAACGACACCA     TTTTGTAGTACTGGTT     TCCCTGAACCCGTCCTT TCTCA-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 001-001765937
Forward Primer: 5'-TTCTAGCCAAGAGGTCCAAAGGCC-3' Tm: 60.908
Reverse Primer: 5'-TGCATGGCCTCTGCATCAGC-3' Tm: 59.508
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 135, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr1:1766045-1766094

Rank: 56	Homology Score: 5	RVD Match Score: 2.887	Left Half-Site RVD Score: 4.754	Right Half-Site RVD Score: 7.343
	Genomic Region: Intergenic	Closest Gene: Pappa
Match Type: L24R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-TTCTC TACCTCCCCACCCCCAT TGCTATGGTTTTGCTCCCTGCATT TCCAGCACAATACAGTA GTGCG-3'
	3'-AAGAG ATGGAGGGGTGGGGGTA ACGATACCAAAACGAGGGACGTAA AGGTCGTGTTATGTCAT CACGC-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 005-082146486
Forward Primer: 5'-GCCAGAGGCCCTGGTCTTAT-3' Tm: 59.508
Reverse Primer: 5'-CTCCATTCCCTACTACAGAGAGTG-3' Tm: 59.038
Expected PCR Product Length: 278
Expected CelI Cleavage Product Sizes: 137, 141
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr5:82146592-82146649

Rank: 57	Homology Score: 2	RVD Match Score: 2.887	Left Half-Site RVD Score: 5.787	Right Half-Site RVD Score: 5.925
	Genomic Region: Intergenic	Closest Gene: Src
Match Type: R17L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-CTTCT CTCATTAATGTTCTGGC    TTTTTTCTGAGGGGAGC     GGGGAGCTGGGGGGGGA GGGAG-3'
	3'-GAAGA GAGTAATTACAAGACCG    AAAAAAGACTCCCCTCG     CCCCTCGACCCCCCCCT CCCTC-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 003-148196736
Forward Primer: 5'-GTGTGTGTGTGTGTGTGTGTGTG-3' Tm: 59.148
Reverse Primer: 5'-AGCCAGAGCATTCTCACTCTCC-3' Tm: 59.258
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 135, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr3:148196844-148196894

Rank: 58	Homology Score: 5	RVD Match Score: 2.888	Left Half-Site RVD Score: 6.198	Right Half-Site RVD Score: 5.592
	Genomic Region: Intron	Closest Gene: Acot7
Match Type: R18L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-TTTTC CTCTCTGTTGTTTTGGT    GATCCACCCAGGGCACTG    TGGGTGCTGGGTAGGTA AGGGC-3'
	3'-AAAAG GAGAGACAACAAAACCA    CTAGGTGGGTCCCGTGAC    ACCCACGACCCATCCAT TCCCG-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 005-169429164
Forward Primer: 5'-GCACACAGCTCTGAGATGTTTGG-3' Tm: 59.148
Reverse Primer: 5'-TGCACTCGGAAAGCTGAGGC-3' Tm: 59.508
Expected PCR Product Length: 272
Expected CelI Cleavage Product Sizes: 142, 130
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr5:169429278-169429329

Rank: 59	Homology Score: 5	RVD Match Score: 2.889	Left Half-Site RVD Score: 5.345	Right Half-Site RVD Score: 6.327
	Genomic Region: Intron	Closest Gene: Nr3c2
Match Type: R23L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-ACAGC CTCAGTGTTGTTCAAGG CCACCCAGAAGTGGACAACAGAT  ATGCAGCTAGGGAGAAG GAAAC-3'
	3'-TGTCG GAGTCACAACAAGTTCC GGTGGGTCTTCACCTGTTGTCTA  TACGTCGATCCCTCTTC CTTTG-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 019-032799375
Forward Primer: 5'-GGGTGAGCAGCTAAGTTTTGGTTC-3' Tm: 59.038
Reverse Primer: 5'-CCTGATCTGATGGGTGTGAGTG-3' Tm: 59.258
Expected PCR Product Length: 366
Expected CelI Cleavage Product Sizes: 165, 201
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr19:32799510-32799566

Rank: 60	Homology Score: 2	RVD Match Score: 2.889	Left Half-Site RVD Score: 5.34	Right Half-Site RVD Score: 6.541
	Genomic Region: Intergenic	Closest Gene: Csrp1
Match Type: L16R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-GCTCT CTCCAACCCAGCTCAGT     ATTGTAGGGAAGCCTC     ACTAGACCAGGACTGTA TCATC-3'
	3'-CGAGA GAGGTTGGGTCGAGTCA     TAACATCCCTTCGGAG     TGATCTGGTCCTGACAT AGTAG-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 013-048702712
Forward Primer: 5'-CGGTCCCCAGCTCTGAAAAAAAG-3' Tm: 59.148
Reverse Primer: 5'-CCAGGAAACTACTGCTTGCTGTTG-3' Tm: 59.038
Expected PCR Product Length: 371
Expected CelI Cleavage Product Sizes: 230, 141
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr13:48702915-48702964

Rank: 61	Homology Score: 5	RVD Match Score: 2.889	Left Half-Site RVD Score: 6.262	Right Half-Site RVD Score: 5.419
	Genomic Region: Intergenic	Closest Gene: Basp1
Match Type: L23R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-TTTCT TTCCATCTCTGCTCCAT TTTTGTATGTGCATTTCCTTTAG  ACAGGAACAATAGTGAG TTAAA-3'
	3'-AAAGA AAGGTAGAGACGAGGTA AAAACATACACGTAAAGGAAATC  TGTCCTTGTTATCACTC AATTT-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 002-074330935
Forward Primer: 5'-CCTGTCTGCGAGCACAATATGAG-3' Tm: 59.148
Reverse Primer: 5'-GAGGCTACTAGGACTCAATGCAG-3' Tm: 59.148
Expected PCR Product Length: 285
Expected CelI Cleavage Product Sizes: 130, 155
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr2:74331035-74331091

Rank: 62	Homology Score: 5	RVD Match Score: 2.891	Left Half-Site RVD Score: 4.322	Right Half-Site RVD Score: 8.064
	Genomic Region: Intergenic	Closest Gene: Rnf217
Match Type: L17R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-AAGAA TTCATACCAAACTCCAT    ATCACCCTGTATATGAC     AACATAGCACCACTAAA AGTCC-3'
	3'-TTCTT AAGTATGGTTTGAGGTA    TAGTGGGACATATACTG     TTGTATCGTGGTGATTT TCAGG-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Warning! Primer pair was generated using suboptimal parameters. 
Primer Index: 001-026132578
Forward Primer: 5'-CATTCAGATACTCTGAAGCGAAACATGTTAAGG-3' Tm: 59.758
Reverse Primer: 5'-CCCCTGACCAATACTTTGCAAACG-3' Tm: 59.038
Expected PCR Product Length: 334
Expected CelI Cleavage Product Sizes: 148, 186
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr1:26132699-26132749

Rank: 63	Homology Score: 2	RVD Match Score: 2.891	Left Half-Site RVD Score: 4.882	Right Half-Site RVD Score: 7.198
	Genomic Region: Intergenic	Closest Gene: Il13ra2
Match Type: L18R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-ATTGA CTCCTTCCAAACTCCCT    CCCCCAAGAACAGCTTCT    CACAGTACAACAGTGTA ACTGT-3'
	3'-TAACT GAGGAAGGTTTGAGGGA    GGGGGTTCTTGTCGAAGA    GTGTCATGTTGTCACAT TGACA-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 00X-030237063
Forward Primer: 5'-TGAAGACCTTCCAGGGGGCT-3' Tm: 59.508
Reverse Primer: 5'-TGTAGCAGACCTCCTGTGGTG-3' Tm: 59.378
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 136, 134
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chrX:30237171-30237222

Rank: 64	Homology Score: 5	RVD Match Score: 2.893	Left Half-Site RVD Score: 5.588	Right Half-Site RVD Score: 6.25
	Genomic Region: Intergenic	Closest Gene: Nipsnap3a
Match Type: L17R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-GCTTC CTTCTGCACAGTTCCAT    GAGTTTGACAAAATCAA     GCAAGAATAACATTAAA CTGAC-3'
	3'-CGAAG GAAGACGTGTCAAGGTA    CTCAAACTGTTTTAGTT     CGTTCTTATTGTAATTT GACTG-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 005-070418635
Forward Primer: 5'-GTGACTGCAGAATTCATCTGCCC-3' Tm: 59.148
Reverse Primer: 5'-GCAGCATATGGGGTTTTCTCACTC-3' Tm: 59.038
Expected PCR Product Length: 275
Expected CelI Cleavage Product Sizes: 132, 143
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr5:70418740-70418790

Rank: 65	Homology Score: 10	RVD Match Score: 2.893	Left Half-Site RVD Score: 6.629	Right Half-Site RVD Score: 5.049
	Genomic Region: Intergenic	Closest Gene: Mir206
Match Type: L23R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-TTTTA TTAGTGCCCAAATCTAT AGTAGTAATGGTCTAATTTGAGC  TCCAGAATAACTCTGAA TCAAA-3'
	3'-AAAAT AATCACGGGTTTAGATA TCATCATTACCAGATTAAACTCG  AGGTCTTATTGAGACTT AGTTT-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 009-019047855
Forward Primer: 5'-GTGGCTTTGGTCCTCATCTGTC-3' Tm: 59.258
Reverse Primer: 5'-CCTCTTTCGGAGATAGGCATGTTC-3' Tm: 59.038
Expected PCR Product Length: 302
Expected CelI Cleavage Product Sizes: 161, 141
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr9:19047986-19048042

Rank: 66	Homology Score: 5	RVD Match Score: 2.894	Left Half-Site RVD Score: 5.348	Right Half-Site RVD Score: 6.57
	Genomic Region: Intron	Closest Gene: Dcc
Match Type: L20R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-AATTC TTGCTACCCAGCCCAAT   ATGGTATTTATATAAAAATA   ATATGATCAACACTGGA TCTAA-3'
	3'-TTAAG AACGATGGGTCGGGTTA   TACCATAAATATATTTTTAT   TATACTAGTTGTGACCT AGATT-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Warning! Primer pair was generated using suboptimal parameters. 
Primer Index: 018-068645313
Forward Primer: 5'-CTGGGAGCCTGGAAATTTGTCTG-3' Tm: 59.148
Reverse Primer: 5'-GGACACAAGCATATATTATTCTTTGGATGTGCTC-3' Tm: 59.668
Expected PCR Product Length: 439
Expected CelI Cleavage Product Sizes: 177, 262
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr18:68645461-68645514

Rank: 67	Homology Score: 10	RVD Match Score: 2.894	Left Half-Site RVD Score: 6.975	Right Half-Site RVD Score: 5.054
	Genomic Region: Intergenic	Closest Gene: Dpp6
Match Type: R15L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-GTCAG TTCTGTGCTGTGCTCAT     GCCATTGTGGGGAAG      AAGGAGATGGGAAGGAA GGTGC-3'
	3'-CAGTC AAGACACGACACGAGTA     CGGTAACACCCCTTC      TTCCTCTACCCTTCCTT CCACG-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 004-003843423
Forward Primer: 5'-GGTGACCAATACCAACCATCAGG-3' Tm: 59.148
Reverse Primer: 5'-CCTCCTTTCAGGAAGGCTCTTTCT-3' Tm: 59.038
Expected PCR Product Length: 319
Expected CelI Cleavage Product Sizes: 169, 150
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr4:3843566-3843614

Rank: 68	Homology Score: 2	RVD Match Score: 2.894	Left Half-Site RVD Score: 5.959	Right Half-Site RVD Score: 5.808
	Genomic Region: Intergenic	Closest Gene: Psma7
Match Type: L24R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-ANTCG TTCCTCCCTCCCTCCCT CTCTTCCCTTCCTCCAAGAATTTC ATCAGAGCAACAAGGAG GAAAG-3'
	3'-TNAGC AAGGAGGGAGGGAGGGA GAGAAGGGAAGGAGGTTCTTAAAG TAGTCTCGTTGTTCCTC CTTTC-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. Warning! The sequence of this region of the genome is poorly mapped. 
Primer Index: 003-168497270
Forward Primer: 5'-ATCTGTCTCCGAGTGCTTCTACG-3' Tm: 59.148
Reverse Primer: 5'-CACAGGTGGGTGATTTCGAAAGC-3' Tm: 59.148
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 135, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr3:168497374-168497431

Rank: 69	Homology Score: 5	RVD Match Score: 2.895	Left Half-Site RVD Score: 6.452	Right Half-Site RVD Score: 5.446
	Genomic Region: Intergenic	Closest Gene: Tmem132b
Match Type: R20L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-GGACC TTCACTGTGATTCTTTT   GTGCATTCAAAAACATCCAC   TTGGGGCTGGGAAGGTA CCTCG-3'
	3'-CCTGG AAGTGACACTAAGAAAA   CACGTAAGTTTTTGTAGGTG   AACCCCGACCCTTCCAT GGAGC-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 012-031851371
Forward Primer: 5'-GGGTGCCTGACTGCTATATTTCC-3' Tm: 59.148
Reverse Primer: 5'-TTGCCTGCCACAACCATGCATC-3' Tm: 59.258
Expected PCR Product Length: 298
Expected CelI Cleavage Product Sizes: 168, 130
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr12:31851510-31851563

Rank: 70	Homology Score: 2	RVD Match Score: 2.896	Left Half-Site RVD Score: 7.335	Right Half-Site RVD Score: 4.823
	Genomic Region: Intergenic	Closest Gene: Nrg2
Match Type: R17L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-TCGAG TTCCGTGACGCTCTGGC    CAAGCTCATCGACGACT     ATGGAGTGGAGGAGGAG CCGGC-3'
	3'-AGCTC AAGGCACTGCGAGACCG    GTTCGAGTAGCTGCTGA     TACCTCACCTCCTCCTC GGCCG-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 018-028922784
Forward Primer: 5'-TCTCAAGGAGAACCAGCGCG-3' Tm: 59.508
Reverse Primer: 5'-GGCTTCACCTCACTGACTCG-3' Tm: 59.508
Expected PCR Product Length: 279
Expected CelI Cleavage Product Sizes: 145, 134
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr18:28922902-28922952

Rank: 71	Homology Score: 2	RVD Match Score: 2.898	Left Half-Site RVD Score: 7.255	Right Half-Site RVD Score: 4.891
	Genomic Region: Intergenic	Closest Gene: Smad2
Match Type: R13L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-CTCTC TTCAGTCATCTGTTGGT      ACTGAGCATGACC       ATGGGGTGGGGTTGGAA GGGTG-3'
	3'-GAGAG AAGTCAGTAGACAACCA      TGACTCGTACTGG       TACCCCACCCCAACCTT CCCAC-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 018-072979833
Forward Primer: 5'-CCGAGTGAAGGTAGCAGAACTCAG-3' Tm: 60.908
Reverse Primer: 5'-TTGCTTAGAGGGACCCAGGAAGA-3' Tm: 59.148
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 135, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr18:72979943-72979989

Rank: 72	Homology Score: 5	RVD Match Score: 2.9	Left Half-Site RVD Score: 6.048	Right Half-Site RVD Score: 5.752
	Genomic Region: Intergenic	Closest Gene: Tfap2a
Match Type: L23R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-ACCCT TTCCTCCCCAACTTGCT TCTTGGTCACGATGTTTTAGCCC  AGCAGTAGAAAACTGAA GCCAC-3'
	3'-TGGGA AAGGAGGGGTTGAACGA AGAACCAGTGCTACAAAATCGGG  TCGTCATCTTTTGACTT CGGTG-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 017-030652372
Forward Primer: 5'-TCCGCTCCTGCACCAGGTTT-3' Tm: 59.508
Reverse Primer: 5'-GGAGTAAGTCAGCATTGAGTGGG-3' Tm: 59.148
Expected PCR Product Length: 370
Expected CelI Cleavage Product Sizes: 130, 240
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr17:30652472-30652528

Rank: 73	Homology Score: 5	RVD Match Score: 2.901	Left Half-Site RVD Score: 6.735	Right Half-Site RVD Score: 5.005
	Genomic Region: Intergenic	Closest Gene: Celf4
Match Type: L19R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-TTTCC TTCAATCCCTTCTCCAT   GTCCTTGTAGCTATTTTAG    ACAAGAACAATTCTGAG TCAGA-3'
	3'-AAAGG AAGTTAGGGAAGAGGTA   CAGGAACATCGATAAAATC    TGTTCTTGTTAAGACTC AGTCT-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 018-019069491
Forward Primer: 5'-GGGTTCCTGCCTATAAGCATACCG-3' Tm: 60.908
Reverse Primer: 5'-GCTAGTGTGTGAAAGACTCCTAGG-3' Tm: 59.038
Expected PCR Product Length: 269
Expected CelI Cleavage Product Sizes: 137, 132
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr18:19069600-19069652

Rank: 74	Homology Score: 5	RVD Match Score: 2.902	Left Half-Site RVD Score: 6.782	Right Half-Site RVD Score: 5.251
	Genomic Region: Intergenic	Closest Gene: Slc7a11
Match Type: R15L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-TTTGT TTCATTTTTGTTTTTGT     TGTGAGAGACAGAAT      ATGAAGTTGGGTGGGTA ATAAG-3'
	3'-AAACA AAGTAAAAACAAAAACA     ACACTCTCTGTCTTA      TACTTCAACCCACCCAT TATTC-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. Warning! The sequence of this region of the genome is poorly mapped. 
Primer Index: 002-139129605
Forward Primer: 5'-CCCATGTGCAGAAGGAGTTGTAC-3' Tm: 59.148
Reverse Primer: 5'-GTTCCAGGTCAGTCAGGGATATAC-3' Tm: 59.038
Expected PCR Product Length: 358
Expected CelI Cleavage Product Sizes: 175, 183
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr2:139129754-139129802

Rank: 75	Homology Score: 5	RVD Match Score: 2.903	Left Half-Site RVD Score: 6.038	Right Half-Site RVD Score: 5.831
	Genomic Region: Intron	Closest Gene: Pde4d
Match Type: R24L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-CCTAA TTCAGTTTGTTTCTAGT AGAGGGAGGGTGAAGGCGCGAAAG CTGGGGGTGGGGGGGAA GCTGC-3'
	3'-GGATT AAGTCAAACAAAGATCA TCTCCCTCCCACTTCCGCGCTTTC GACCCCCACCCCCCCTT CGACG-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 002-040496981
Forward Primer: 5'-GTGTGTGTGTGTGTGTGTGTGG-3' Tm: 59.258
Reverse Primer: 5'-TCTTCCACACCCTGTGCACC-3' Tm: 59.508
Expected PCR Product Length: 273
Expected CelI Cleavage Product Sizes: 142, 131
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr2:40497092-40497149

Rank: 76	Homology Score: 2	RVD Match Score: 2.903	Left Half-Site RVD Score: 6.688	Right Half-Site RVD Score: 5.325
	Genomic Region: Intergenic	Closest Gene: Ferd3l
Match Type: R18L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-CAATT TTGACTGATGTCCTGAT    CTAGAGGGAGGAAAGAAA    GAGGAGTGGGGTAGGAA ATAGA-3'
	3'-GTTAA AACTGACTACAGGACTA    GATCTCCCTCCTTTCTTT    CTCCTCACCCCATCCTT TATCT-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 006-052366880
Forward Primer: 5'-GGGGGAGGGAACCTTAATAGGA-3' Tm: 59.258
Reverse Primer: 5'-CAGAAAGTGCTGAGCCAAGTGAC-3' Tm: 59.148
Expected PCR Product Length: 434
Expected CelI Cleavage Product Sizes: 300, 134
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr6:52367152-52367203

Rank: 77	Homology Score: 2	RVD Match Score: 2.903	Left Half-Site RVD Score: 6.034	Right Half-Site RVD Score: 5.796
	Genomic Region: Intergenic	Closest Gene: Plp1
Match Type: L22R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-CTGAG TCCTTCCCCACCTACAT  CCTCTCCAGAAGCTATCAGTTA  TGGAGAACTACACTGAG CATCC-3'
	3'-GACTC AGGAAGGGGTGGATGTA  GGAGAGGTCTTCGATAGTCAAT  ACCTCTTGATGTGACTC GTAGG-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 00X-124470518
Forward Primer: 5'-CCTTGTGACCACCCCCAAGA-3' Tm: 59.508
Reverse Primer: 5'-ACTGTGGGGAAAAGGATCTTGGC-3' Tm: 59.148
Expected PCR Product Length: 279
Expected CelI Cleavage Product Sizes: 149, 130
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chrX:124470637-124470692

Rank: 78	Homology Score: 5	RVD Match Score: 2.903	Left Half-Site RVD Score: 5.84	Right Half-Site RVD Score: 6.029
	Genomic Region: Intergenic	Closest Gene: Slc15a5
Match Type: L20R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-CGTTA TTAATGCACAGCCCCAT   AAAAACATTGATTTAGAGGA   ACCTTTATAACATTGAA GTGCA-3'
	3'-GCAAT AATTACGTGTCGGGGTA   TTTTTGTAACTAAATCTCCT   TGGAAATATTGTAACTT CACGT-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 004-174994043
Forward Primer: 5'-GTGCGGCTCTGTCCTCACAT-3' Tm: 59.508
Reverse Primer: 5'-GATTAAATTGCATGCAGCTGCACTAACTCC-3' Tm: 60.058
Expected PCR Product Length: 269
Expected CelI Cleavage Product Sizes: 138, 131
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr4:174994152-174994205

Rank: 79	Homology Score: 2	RVD Match Score: 2.904	Left Half-Site RVD Score: 5.893	Right Half-Site RVD Score: 5.979
	Genomic Region: Intergenic	Closest Gene: Cachd1
Match Type: L16R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-TCCAC CTCCTTCCTTCCTCCAT     CACCATCACCACCATC     ATGAGAATAACTCAGAA GACCC-3'
	3'-AGGTG GAGGAAGGAAGGAGGTA     GTGGTAGTGGTGGTAG     TACTCTTATTGAGTCTT CTGGG-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 005-121313865
Forward Primer: 5'-GATAAACTCATGCTCTGGGGTTGG-3' Tm: 59.038
Reverse Primer: 5'-GCTCTCCATGGAGGCTAGTG-3' Tm: 59.508
Expected PCR Product Length: 326
Expected CelI Cleavage Product Sizes: 131, 195
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr5:121313969-121314018

Rank: 80	Homology Score: 2	RVD Match Score: 2.906	Left Half-Site RVD Score: 5.759	Right Half-Site RVD Score: 6.09
	Genomic Region: Intron	Closest Gene: Arhgap15
Match Type: R17L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-TTTTT CTCAATGCTGGTCTCGT    GGAGATTGTATGACCTT     AGAGGGCTTGGTAGGAA AATTA-3'
	3'-AAAAA GAGTTACGACCAGAGCA    CCTCTAACATACTGGAA     TCTCCCGAACCATCCTT TTAAT-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 003-024415174
Forward Primer: 5'-TTTCCCATCCTGCCATGCCAC-3' Tm: 59.378
Reverse Primer: 5'-GGCTTACCAATGTACGACTGTGC-3' Tm: 59.148
Expected PCR Product Length: 318
Expected CelI Cleavage Product Sizes: 130, 188
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr3:24415277-24415327

Rank: 81	Homology Score: 5	RVD Match Score: 2.906	Left Half-Site RVD Score: 7.456	Right Half-Site RVD Score: 4.348
	Genomic Region: Intergenic	Closest Gene: Aldh8a1
Match Type: L23R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-TTTCT TTCAGTCTCTGCTCCAT TTTTGTCCCTGAATGTTCTTTAG  ACTAGAACAATTCTGAG TAAAC-3'
	3'-AAAGA AAGTCAGAGACGAGGTA AAAACAGGGACTTACAAGAAATC  TGATCTTGTTAAGACTC ATTTG-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Acceptable Primer Pair Not Found!
Left Primers Found: 270
Right Primers Found: 19
Primers may not have been generated due to repetitive genomic sequence.
Primer pairs may not have been generated since primer dimers would form or product sizes are incompatible with specified parameters.
A careful manual examination of the genomic sequence may allow design of primers suitable for amplification of desired product.
View in Genome Browser, Chromosomal Coordinates - chr1:16859003-16859059

Rank: 82	Homology Score: 10	RVD Match Score: 2.91	Left Half-Site RVD Score: 4.664	Right Half-Site RVD Score: 7.713
	Genomic Region: Intergenic	Closest Gene: Zfp385b
Match Type: L19R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-TTTCC TTCCAGCCCATCTCCAC   TTCTTTATGCCAGCACTGA    TCCACAGAAACACTGTA AACCA-3'
	3'-AAAGG AAGGTCGGGTAGAGGTG   AAGAAATACGGTCGTGACT    AGGTGTCTTTGTGACAT TTGGT-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 003-060396362
Forward Primer: 5'-CCACTGGGAGTGTATTCGGTATG-3' Tm: 59.148
Reverse Primer: 5'-GTAAACAGGCCTCTACAGAACGG-3' Tm: 59.148
Expected PCR Product Length: 362
Expected CelI Cleavage Product Sizes: 230, 132
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr3:60396564-60396616

Rank: 83	Homology Score: 5	RVD Match Score: 2.91	Left Half-Site RVD Score: 5.135	Right Half-Site RVD Score: 7.019
	Genomic Region: Intergenic	Closest Gene: LOC654482
Match Type: L19R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-CTGAG TTCTTCCCCACCTCCAC   CTCTGCCAGAAGGCAATTG    TGAAGAACTACACTTAA GTATT-3'
	3'-GACTC AAGAAGGGGTGGAGGTG   GAGACGGTCTTCCGTTAAC    ACTTCTTGATGTGAATT CATAA-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. Warning! The sequence of this region of the genome is poorly mapped. 
Primer Index: 008-005163674
Forward Primer: 5'-AGGAGGAGGAGGAGGAGGAA-3' Tm: 59.508
Reverse Primer: 5'-TATATGTGGCTGGAGTCACAGGTC-3' Tm: 59.038
Expected PCR Product Length: 264
Expected CelI Cleavage Product Sizes: 132, 132
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr8:5163778-5163830

Rank: 84	Homology Score: 2	RVD Match Score: 2.91	Left Half-Site RVD Score: 5.754	Right Half-Site RVD Score: 6.201
	Genomic Region: Intergenic	Closest Gene: Tgfbr2
Match Type: L13R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-GCGCC TCCCTCCCCAACTACTT      CCCTTTCCTCATC       TCCAGATGAATAATGAA GTCTG-3'
	3'-CGCGG AGGGAGGGGTTGATGAA      GGGAAAGGAGTAG       AGGTCTACTTATTACTT CAGAC-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 008-120556664
Forward Primer: 5'-CAATTGAATCAGGACCCGGTTTGG-3' Tm: 59.038
Reverse Primer: 5'-GGTCAGTAATGATGGGTCTCTCC-3' Tm: 59.148
Expected PCR Product Length: 310
Expected CelI Cleavage Product Sizes: 153, 157
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr8:120556792-120556838

Rank: 85	Homology Score: 2	RVD Match Score: 2.913	Left Half-Site RVD Score: 5.404	Right Half-Site RVD Score: 6.68
	Genomic Region: Intergenic	Closest Gene: Hs3st3b1
Match Type: L18R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-GCTTC CTTCTGCAAAACTCCAT    CTGAAGTAACTCCTAGGA    ACCAGGGTAACAGAGAA CTTAG-3'
	3'-CGAAG GAAGACGTTTTGAGGTA    GACTTCATTGAGGATCCT    TGGTCCCATTGTCTCTT GAATC-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 010-049832087
Forward Primer: 5'-CCCTGTTCCCACTTTCCTTCTTAG-3' Tm: 59.038
Reverse Primer: 5'-GCCTTACAGGAAAATGGTGACCTC-3' Tm: 59.038
Expected PCR Product Length: 321
Expected CelI Cleavage Product Sizes: 184, 137
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr10:49832243-49832294

Rank: 86	Homology Score: 2	RVD Match Score: 2.913	Left Half-Site RVD Score: 5.404	Right Half-Site RVD Score: 6.68
	Genomic Region: Intergenic	Closest Gene: Pde4d
Match Type: L18R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-GCTTC CTTCTGCAAAACTCCAT    CTGAAGTAACTCCTAGGA    ACCAGGGTAACAGAGAA CTTAG-3'
	3'-CGAAG GAAGACGTTTTGAGGTA    GACTTCATTGAGGATCCT    TGGTCCCATTGTCTCTT GAATC-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: dom-000053994
Forward Primer: 5'-CCCTGTTCCCACTTTCCTTCTTAG-3' Tm: 59.038
Reverse Primer: 5'-GCCTTACAGGAAAATGGTGACCTC-3' Tm: 59.038
Expected PCR Product Length: 321
Expected CelI Cleavage Product Sizes: 184, 137
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr18_random:54150-54201

Rank: 87	Homology Score: 5	RVD Match Score: 2.913	Left Half-Site RVD Score: 6.113	Right Half-Site RVD Score: 5.848
	Genomic Region: Intergenic	Closest Gene: Tmub1
Match Type: R23L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-AGCTA TTCAATCTTTTTCTTTT AGGGTTTTTCCCCCTCATCTGTC  AGGGAAATGGGCAGGGA AAAGG-3'
	3'-TCGAT AAGTTAGAAAAAGAAAA TCCCAAAAAGGGGGAGTAGACAG  TCCCTTTACCCGTCCCT TTTCC-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 004-006079924
Forward Primer: 5'-GTGTTCTACCCTCAGCCCAACAA-3' Tm: 59.148
Reverse Primer: 5'-GGGAAGGGCACACTGTTTATTACC-3' Tm: 59.038
Expected PCR Product Length: 316
Expected CelI Cleavage Product Sizes: 142, 174
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr4:6080036-6080092

Rank: 88	Homology Score: 2	RVD Match Score: 2.913	Left Half-Site RVD Score: 6.73	Right Half-Site RVD Score: 5.369
	Genomic Region: Intergenic	Closest Gene: Jph4
Match Type: R18L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-GAGGC TTAAGTGTTGATCCAGA    TGACAGCAGGGTGGTTTC    AGGGACCTGTGTAGGAG TAGAA-3'
	3'-CTCCG AATTCACAACTAGGTCT    ACTGTCGTCCCACCAAAG    TCCCTGGACACATCCTC ATCTT-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 015-033211731
Forward Primer: 5'-ACCAGGAGGAGCAGGGTGAA-3' Tm: 59.508
Reverse Primer: 5'-GCCTCACCCTGCCCAGTTAA-3' Tm: 59.508
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 134, 136
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr15:33211837-33211888

Rank: 89	Homology Score: 2	RVD Match Score: 2.914	Left Half-Site RVD Score: 7.107	Right Half-Site RVD Score: 5.104
	Genomic Region: Intergenic	Closest Gene: Tas2r138
Match Type: R23L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-ATCTG TTAAGTGTTCTTAGGCT ATCTGGAGTTTCAATCCTCTAAA  ATGTTGTTTGGTAGGAA TTCAA-3'
	3'-TAGAC AATTCACAAGAATCCGA TAGACCTCAAAGTTAGGAGATTT  TACAACAAACCATCCTT AAGTT-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 004-068260584
Forward Primer: 5'-CCCGTCTCTGTCAAACATGCTAAG-3' Tm: 59.038
Reverse Primer: 5'-GGAACTTCATAACGACTGCGGTTG-3' Tm: 59.038
Expected PCR Product Length: 311
Expected CelI Cleavage Product Sizes: 180, 131
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr4:68260734-68260790

Rank: 90	Homology Score: 2	RVD Match Score: 2.914	Left Half-Site RVD Score: 7.107	Right Half-Site RVD Score: 5.104
	Genomic Region: Intergenic	Closest Gene: Tas2r138
Match Type: R23L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-ATCTG TTAAGTGTTCTTAGGCT ATCCGGAGTTTCAATCCTCTAAA  ATGTTGTTTGGTAGGAA TTCAA-3'
	3'-TAGAC AATTCACAAGAATCCGA TAGGCCTCAAAGTTAGGAGATTT  TACAACAAACCATCCTT AAGTT-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 004-068285787
Forward Primer: 5'-GAGTGTGCTGAGTCACTCATCTCT-3' Tm: 59.038
Reverse Primer: 5'-GGAACTTCATAACGACTGCGGTTG-3' Tm: 59.038
Expected PCR Product Length: 288
Expected CelI Cleavage Product Sizes: 157, 131
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr4:68285914-68285970

Rank: 91	Homology Score: 8	RVD Match Score: 2.915	Left Half-Site RVD Score: 5.426	Right Half-Site RVD Score: 6.453
	Genomic Region: Intergenic	Closest Gene: Prdm2
Match Type: R19L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-TACCT TTCAGGGTTGTTCTAAA   AACTAACTGGAGAACACAA    ATGGCGCCTGGCATGAA GTAAC-3'
	3'-ATGGA AAGTCCCAACAAGATTT   TTGATTGACCTCTTGTGTT    TACCGCGGACCGTACTT CATTG-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 005-161756246
Forward Primer: 5'-CTCTAGGGGCTAAGTATCTCAGCT-3' Tm: 59.038
Reverse Primer: 5'-GGTGGCAAAATCTAAACCCTGTGG-3' Tm: 59.038
Expected PCR Product Length: 280
Expected CelI Cleavage Product Sizes: 130, 150
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr5:161756348-161756400

Rank: 92	Homology Score: 2	RVD Match Score: 2.915	Left Half-Site RVD Score: 5.333	Right Half-Site RVD Score: 6.798
	Genomic Region: Intergenic	Closest Gene: Brms1l
Match Type: L24R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-CTCTG TTGCTACCCAACTCCCC ACACCAGCAGTGGTACCTTAAGGG CCCAGCATAACATTGCA AACTC-3'
	3'-GAGAC AACGATGGGTTGAGGGG TGTGGTCGTCACCATGGAATTCCC GGGTCGTATTGTAACGT TTGAG-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. Warning! The sequence of this region of the genome is poorly mapped. 
Primer Index: 006-076453434
Forward Primer: 5'-GGACCATTGTCAAATCAGGTGGG-3' Tm: 59.148
Reverse Primer: 5'-GCCAGGGTGCCATGATTCCTA-3' Tm: 59.378
Expected PCR Product Length: 418
Expected CelI Cleavage Product Sizes: 288, 130
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr6:76453691-76453748

Rank: 93	Homology Score: 5	RVD Match Score: 2.916	Left Half-Site RVD Score: 6.488	Right Half-Site RVD Score: 5.569
	Genomic Region: Intergenic	Closest Gene: Cdh8
Match Type: R16L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-TTCAG TTCAGTGCAGTTCAGTT     TGAAACAGCTCACTTC     ATGGAGTTGAGAAGCAG TCTTT-3'
	3'-AAGTC AAGTCACGTCAAGTCAA     ACTTTGTCGAGTGAAG     TACCTCAACTCTTCGTC AGAAA-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 019-006170213
Forward Primer: 5'-GAGAAAGGTTTGGCAGAACGAGTC-3' Tm: 59.038
Reverse Primer: 5'-CCAGCTGGCTGGTCCAAATC-3' Tm: 59.508
Expected PCR Product Length: 305
Expected CelI Cleavage Product Sizes: 175, 130
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr19:6170361-6170410

Rank: 94	Homology Score: 8	RVD Match Score: 2.918	Left Half-Site RVD Score: 6.804	Right Half-Site RVD Score: 5.348
	Genomic Region: Intergenic	Closest Gene: Stk17b
Match Type: R21L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-TGGCA TTCCGTTTGGTCCTGGT  TACTCCTGCTGACTCATTAGA   ATTGAGCTGGCTGGGAA GGTAA-3'
	3'-ACCGT AAGGCAAACCAGGACCA  ATGAGGACGACTGAGTAATCT   TAACTCGACCGACCCTT CCATT-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 009-052013154
Forward Primer: 5'-GAGGCTGAGCTTGGCTTGCTT-3' Tm: 59.378
Reverse Primer: 5'-GCAAGGTCACATAGTGTCACTTGG-3' Tm: 59.038
Expected PCR Product Length: 311
Expected CelI Cleavage Product Sizes: 157, 154
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr9:52013282-52013336

Rank: 95	Homology Score: 2	RVD Match Score: 2.92	Left Half-Site RVD Score: 5.284	Right Half-Site RVD Score: 6.62
	Genomic Region: Intergenic	Closest Gene: Pcdh20
Match Type: R13L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-ACATT TTAAATTTTGTTCTAAT      GTTAAAACAATGC       AAGGATCTGGGTATAAA AATAT-3'
	3'-TGTAA AATTTAAAACAAGATTA      CAATTTTGTTACG       TTCCTAGACCCATATTT TTATA-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 015-073002183
Forward Primer: 5'-CCTTGCTCTCCATCCCACAC-3' Tm: 59.508
Reverse Primer: 5'-GAGTCTTGGGTCTGATTGCTTGG-3' Tm: 59.148
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 135, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr15:73002293-73002339

Rank: 96	Homology Score: 2	RVD Match Score: 2.92	Left Half-Site RVD Score: 6.069	Right Half-Site RVD Score: 5.913
	Genomic Region: Intron	Closest Gene: LOC246295
Match Type: L20R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-TGTGC TTTCCACACAGCACCAT   GCAGCCTCAGTCACCAGGCC   TCTAGAACAGCATTGTA ACAGG-3'
	3'-ACACG AAAGGTGTGTCGTGGTA   CGTCGGAGTCAGTGGTCCGG   AGATCTTGTCGTAACAT TGTCC-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 005-170773963
Forward Primer: 5'-CCAAGGTTGCTGCCAGTTCAGCT-3' Tm: 61.088
Reverse Primer: 5'-AGGAGCTGGAGAGCCAAGTG-3' Tm: 59.508
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 135, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr5:170774069-170774122

Rank: 97	Homology Score: 8	RVD Match Score: 2.922	Left Half-Site RVD Score: 6.199	Right Half-Site RVD Score: 5.84
	Genomic Region: Intergenic	Closest Gene: Actrt1
Match Type: R14L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-ATTTA TTAATTGTTGGTCTGAT      TACCTATGTTATTA      ATGGTTCTGTTCAGGAA GTTTT-3'
	3'-TAAAT AATTAACAACCAGACTA      ATGGATACAATAAT      TACCAAGACAAGTCCTT CAAAA-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Warning! Primer pair was generated using suboptimal parameters. 
Primer Index: 00X-131557987
Forward Primer: 5'-TTTTGGATGTCAGCTATAGGGTTGGAGAAAATCTT-3' Tm: 59.578
Reverse Primer: 5'-CAACAAATGATGCTAATCTAACTGGATGTCTACATG-3' Tm: 59.498
Expected PCR Product Length: 337
Expected CelI Cleavage Product Sizes: 131, 206
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chrX:131558092-131558139

Rank: 98	Homology Score: 2	RVD Match Score: 2.922	Left Half-Site RVD Score: 6.415	Right Half-Site RVD Score: 5.673
	Genomic Region: Intergenic	Closest Gene: Bcor
Match Type: R22L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-GGAGT CTCAGTGCTCTTCTGTG  GCCCCAGAGAGACTATCTGTAT  ATGTGGCAGGGGAGGAG GCAGG-3'
	3'-CCTCA GAGTCACGAGAAGACAC  CGGGGTCTCTCTGATAGACATA  TACACCGTCCCCTCCTC CGTCC-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 00X-022942491
Forward Primer: 5'-GGCAAGCCAAAGGAGTCTCCTG-3' Tm: 61.288
Reverse Primer: 5'-TCTCAGTGCCAGCTCGTCCA-3' Tm: 59.508
Expected PCR Product Length: 278
Expected CelI Cleavage Product Sizes: 148, 130
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chrX:22942609-22942664

Rank: 99	Homology Score: 5	RVD Match Score: 2.924	Left Half-Site RVD Score: 7.522	Right Half-Site RVD Score: 4.884
	Genomic Region: Intergenic	Closest Gene: RGD1561065
Match Type: R24L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-GGATA TGCATTGATGTACTGTT GCTGTGACAAAATTCTATGACCAA AGCGAGTTGGGGAGGAA AGGGT-3'
	3'-CCTAT ACGTAACTACATGACAA CGACACTGTTTTAAGATACTGGTT TCGCTCAACCCCTCCTT TCCCA-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 00X-040773744
Forward Primer: 5'-GGGTGAAACAGCATGTGGCATC-3' Tm: 59.258
Reverse Primer: 5'-GGTCCTATTAACTCCTACCTCCAG-3' Tm: 59.038
Expected PCR Product Length: 441
Expected CelI Cleavage Product Sizes: 310, 131
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chrX:40774023-40774080

Rank: 100	Homology Score: 2	RVD Match Score: 2.924	Left Half-Site RVD Score: 6.902	Right Half-Site RVD Score: 5.32
	Genomic Region: Intron	Closest Gene: Cubn
Match Type: R17L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-AAATA TTCACAGTTTTTCAGGA    TGATCAATGAAACAACA     ATGGGGTTTGGGGGGAA TACTG-3'
	3'-TTTAT AAGTGTCAAAAAGTCCT    ACTAGTTACTTTGTTGT     TACCCCAAACCCCCCTT ATGAC-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 017-087721857
Forward Primer: 5'-CCAAGGATTTCCTGACATCGTGTG-3' Tm: 59.038
Reverse Primer: 5'-TGTGAGAAACCTGGGTGAGCC-3' Tm: 59.378
Expected PCR Product Length: 273
Expected CelI Cleavage Product Sizes: 142, 131
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr17:87721972-87722022

Plate format Oligo Order Form
Well
Name
Sequence
5' mods
Internal
Notes
Primer Plate #1
A1
006-138443970F
GGAATCGGCACATGCAGATCTTTG
Tesson_IgM_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
A2
006-138443970R
TCTGGTCCTCAGAGAGTCAGTC
Tesson_IgM_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
B1
001-256112389F
CCTGGATAAGTCAGTAAAGGGTGG
Tesson_IgM_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
B2
001-256112389R
CCTTGGTGGGACCTGATACAGT
Tesson_IgM_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
C1
003-149194978F
CTTGTTAGGTGAAGCAGGATGGC
Tesson_IgM_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
C2
003-149194978R
TCACACCGGTGCTGAAGGAAC
Tesson_IgM_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
D1
006-046986724F
CTTCTGGCTTAGTTTCCGTCTGTC
Tesson_IgM_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
D2
006-046986724R
CTCAGCTTGCCCAAGGGTTATAG
Tesson_IgM_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
E1
001-252428669F
GTTGTGTTTCAGGACAGCACTACC
Tesson_IgM_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
E2
001-252428669R
GTTGGAGAGGCCACATTCTTAACC
Tesson_IgM_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
F1
007-139879252F
AAAGGCTCATTAGACACGCTCAGG
Tesson_IgM_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
F2
007-139879252R
CACGTCTCTGCAGACCCCAT
Tesson_IgM_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
G1
003-151465311F
CACACACACACACACACACACAC
Tesson_IgM_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
G2
003-151465311R
CACCAGTGACAACAGCACTTGAG
Tesson_IgM_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
H1
00X-027254372F
CCATTATCGCTAGCCTAGCTTACG
Tesson_IgM_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
H2
00X-027254372R
GACTGGGAATAAGTGGTGTGGTAC
Tesson_IgM_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
A3
001-189623849F
TGCATTCCCACCCCCATACTG
Tesson_IgM_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
A4
001-189623849R
CTGAGCACATTCAGGCATCTGG
Tesson_IgM_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
B3
007-062964516F
TCAAGGCGCAGTCAACCTCC
Tesson_IgM_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
B4
007-062964516R
CTGTCAAGGTTGAACAAGGGACTG
Tesson_IgM_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
C3
009-007190270F
AGTTGGGCTGGTAGAAATTGCTGG
Tesson_IgM_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
C4
009-007190270R
CTGAGTGAAACAAACGGCTCCGA
Tesson_IgM_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
D3
005-036123844F
GGCTGTAGACACTCTTCAGCTTG
Tesson_IgM_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
D4
005-036123844R
GTGACCTCTCTGCAGACAGC
Tesson_IgM_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
E3
006-090557385F
GTCCCCACAGGCATATGATCG
Tesson_IgM_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
E4
006-090557385R
GTGCTTCGGGCAGTAGAGCT
Tesson_IgM_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
F3
017-089324125F
GGATTTGGGAGAGGAAGGATGAG
Tesson_IgM_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
F4
017-089324125R
CATAGTGACACACCCTGAAGTTGC
Tesson_IgM_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
G3
014-008802083F
TGCCAGGCTCCTGTCTGCAT
Tesson_IgM_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
G4
014-008802083R
GCTACCAGGACTCAATGGGG
Tesson_IgM_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
H3
0Un-024874250F
GCCTTCAATTCAGTGAGGAATGGG
Tesson_IgM_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
H4
0Un-024874250R
TACTCAAGTGTGTGCCATGCACC
Tesson_IgM_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
A5
020-043706993F
GCAGAAATGGATAGTTTGTGGGGG
Tesson_IgM_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. Warning! The sequence of this region of the genome is poorly mapped. 
A6
020-043706993R
CCGCCAAGCCCTTTTTAAGTACTG
Tesson_IgM_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. Warning! The sequence of this region of the genome is poorly mapped. 
B5
004-126653383F
CCATGTGACCTTTGGACTACATGC
Tesson_IgM_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
B6
004-126653383R
GAGAGCCACTGACTACCATAACAC
Tesson_IgM_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
C5
00X-074124866F
AATGCCCTACAGTGGGGAGAG
Tesson_IgM_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
C6
00X-074124866R
CAAAGGACAGTCATGATAGGTCCC
Tesson_IgM_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
D5
014-082098705F
AGCCAAGGAAGAAACCTGTGCAGC
Tesson_IgM_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
D6
014-082098705R
CTGTGGATGGCTAGAGTGTACTCA
Tesson_IgM_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
E5
019-057896612F
CCCAGTGCTGGGGTTACAAACA
Tesson_IgM_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
E6
019-057896612R
TCTTAGCCAGGAATGAGGCTGC
Tesson_IgM_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
F5
005-123636672F
CTCTGAGTGACACGAATTAGTGGG
Tesson_IgM_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
F6
005-123636672R
GGAGTGTGTCACTAAGGCTGG
Tesson_IgM_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
G5
003-128243778F
CAACCACACTGCAGACCAGG
Tesson_IgM_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
G6
003-128243778R
GCCATGGATCTGATATTGGTGGCT
Tesson_IgM_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
H5
003-100952790F
GCCATGAAGAAGACATGCAGGAG
Tesson_IgM_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
H6
003-100952790R
GGATCACTTGAAATGTGGAGCTTCCTAC
Tesson_IgM_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
A7
001-138291107F
TATCTATGTGGCAGGCTGGGTAC
Tesson_IgM_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
A8
001-138291107R
GTGCAGTGTCATACCCGGGTT
Tesson_IgM_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
B7
012-027291771F
TCCTAAGGGTGGCCTTTTGACCTC
Tesson_IgM_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
B8
012-027291771R
CAGCCTCAGCTCACAGAGATC
Tesson_IgM_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
C7
020-016460972F
GTCAAGGACGAAGTCATCTGGAG
Tesson_IgM_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
C8
020-016460972R
GACAAGCTGACTGCTGCTAGAG
Tesson_IgM_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
D7
020-014621051F
GCCTGTGGTTGGTACATGTACCT
Tesson_IgM_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
D8
020-014621051R
GAGCCGTGATTCTCCATGGATTC
Tesson_IgM_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
E7
007-038298476F
AAAAGCATGAGCTCCTCAGGCTGG
Tesson_IgM_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
E8
007-038298476R
GAAGAGGAAACTGTGGTCAGGTTG
Tesson_IgM_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
F7
002-204001007F
AATTTATGGGCTCTGTGCCGTGC
Tesson_IgM_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
F8
002-204001007R
CCCAGTGCTAAGTTGAGTGTGGC
Tesson_IgM_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
G7
016-077159880F
CTGTGGAGTTTGTGCAGGAATGCT
Tesson_IgM_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
G8
016-077159880R
GGGGCAGTGTGTGATCAAAAGG
Tesson_IgM_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
H7
003-157836620F
GGCAAAGCTGCCTGTCACCA
Tesson_IgM_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
H8
003-157836620R
GCTTCAGGCACAGCTGGATC
Tesson_IgM_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
A9
002-159432579F
GCTAAATCCCCAACCCTGCAG
Tesson_IgM_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
A10
002-159432579R
GTGTTTACTTGCTCAAACACCCGG
Tesson_IgM_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
B9
018-071340665F
CCCCCATCCCCTTTTACAGTTC
Tesson_IgM_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
B10
018-071340665R
GTAGAGGTCCCTCCTTGAGATTC
Tesson_IgM_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
C9
013-038906135F
GCCCCACAGCTATCTCTTTTTGGA
Tesson_IgM_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
C10
013-038906135R
CTGGAGACAGCTTTCAAATCACCC
Tesson_IgM_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
D9
001-127719260F
CCAACATTCTCCCAAGAGATTCTGGAAAC
Tesson_IgM_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
D10
001-127719260R
GTTCCAGGTCAACCAGGACTAC
Tesson_IgM_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
E9
005-151331548F
CTGACCCAGGGCAAGTCATTTAC
Tesson_IgM_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
E10
005-151331548R
GGACCTCCAACCAGATGAACTAAC
Tesson_IgM_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
F9
006-075907048F
GGTGTCCTCATTCACCTCTGACAA
Tesson_IgM_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
F10
006-075907048R
GTGTTTGGAACATCCTCTGCGAC
Tesson_IgM_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
G9
001-140972716F
GGGACCACCTCAGCTAAGGA
Tesson_IgM_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
G10
001-140972716R
GTGGGGTTGTTGGTTTTCAGCTC
Tesson_IgM_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
H9
No PrimersF
No Primers
Tesson_IgM_Ranking-RVDs_5TC	No Primers
H10
No PrimersR
No Primers
Tesson_IgM_Ranking-RVDs_5TC	No Primers
A11
010-102159896F
GGAACCACCCATTTCCTTGGTGT
Tesson_IgM_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
A12
010-102159896R
GTGTGTCTGTCTGTCTGTCTGTC
Tesson_IgM_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
B11
020-049001036F
GGGGGAGGAGAGTAGTAAATTCG
Tesson_IgM_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
B12
020-049001036R
TTCGCGACTTGCCGACGAGA
Tesson_IgM_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
C11
dom-000644656F
GGAGAGAGAAAGGGCGATAGG
Tesson_IgM_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
C12
dom-000644656R
GCACCTGAGATAAAAGACCCTCTG
Tesson_IgM_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
D11
00X-016509931F
CTACCAATGTCTATGGAGTGACCG
Tesson_IgM_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
D12
00X-016509931R
GCCTAGAGTATCCCAAATGTGCAC
Tesson_IgM_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
E11
004-135905706F
GACAACGACTGCGTTCTATTTGGG
Tesson_IgM_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
E12
004-135905706R
TCACGAGCCCTGGTTATTGTTTGG
Tesson_IgM_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
F11
007-067524783F
TGATCAGAGCCTGCCCCTTC
Tesson_IgM_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
F12
007-067524783R
GACAGGGTGCTTCCTCACAATAC
Tesson_IgM_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
G11
010-013862379F
CATAGGTGGGGCCTTATCAGAG
Tesson_IgM_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
G12
010-013862379R
GGTCCCTAGTCAAGGACATTGTCT
Tesson_IgM_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
H11
006-086619661F
GGCTTCAGGAAAAGGTTGATTCCC
Tesson_IgM_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
H12
006-086619661R
CGTGAAGGAGTCTCGTGTCAAC
Tesson_IgM_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
Primer Plate #2
A1
009-054998586F
CTCTCTCTCACGGGGTCTAG
Tesson_IgM_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
A2
009-054998586R
AGTTCTGAGAGTCTCTCACCTCC
Tesson_IgM_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
B1
020-051519222F
GTGTTCCTGAACTCTAGTAGAGGC
Tesson_IgM_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
B2
020-051519222R
TAGCAGAACTCCTGCCCGTG
Tesson_IgM_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
C1
007-135956125F
CACTCCTACGTAGGTATCTTGGG
Tesson_IgM_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
C2
007-135956125R
CTCACCAGAAGAGGGCATCAAATC
Tesson_IgM_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
D1
009-094128198F
GTGACACTGGGAAAATCAGTAGGC
Tesson_IgM_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
D2
009-094128198R
GGTGTGTCTCTGGCTCTTTTGC
Tesson_IgM_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
E1
005-080218256F
TCTCCCTAAGAGAAGGGGTGG
Tesson_IgM_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
E2
005-080218256R
GTCACTGCTTACACTGAATGACCC
Tesson_IgM_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
F1
003-124119953F
CAGTAGCTGCCTTTGACCAGGA
Tesson_IgM_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
F2
003-124119953R
CAAACCAGACAATGGCTTCTGCTC
Tesson_IgM_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
G1
001-001765937F
TTCTAGCCAAGAGGTCCAAAGGCC
Tesson_IgM_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
G2
001-001765937R
TGCATGGCCTCTGCATCAGC
Tesson_IgM_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
H1
005-082146486F
GCCAGAGGCCCTGGTCTTAT
Tesson_IgM_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
H2
005-082146486R
CTCCATTCCCTACTACAGAGAGTG
Tesson_IgM_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
A3
003-148196736F
GTGTGTGTGTGTGTGTGTGTGTG
Tesson_IgM_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
A4
003-148196736R
AGCCAGAGCATTCTCACTCTCC
Tesson_IgM_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
B3
005-169429164F
GCACACAGCTCTGAGATGTTTGG
Tesson_IgM_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
B4
005-169429164R
TGCACTCGGAAAGCTGAGGC
Tesson_IgM_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
C3
019-032799375F
GGGTGAGCAGCTAAGTTTTGGTTC
Tesson_IgM_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
C4
019-032799375R
CCTGATCTGATGGGTGTGAGTG
Tesson_IgM_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
D3
013-048702712F
CGGTCCCCAGCTCTGAAAAAAAG
Tesson_IgM_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
D4
013-048702712R
CCAGGAAACTACTGCTTGCTGTTG
Tesson_IgM_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
E3
002-074330935F
CCTGTCTGCGAGCACAATATGAG
Tesson_IgM_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
E4
002-074330935R
GAGGCTACTAGGACTCAATGCAG
Tesson_IgM_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
F3
001-026132578F
CATTCAGATACTCTGAAGCGAAACATGTTAAGG
Tesson_IgM_Ranking-RVDs_5TC	Warning! Primer pair was generated using suboptimal parameters. 
F4
001-026132578R
CCCCTGACCAATACTTTGCAAACG
Tesson_IgM_Ranking-RVDs_5TC	Warning! Primer pair was generated using suboptimal parameters. 
G3
00X-030237063F
TGAAGACCTTCCAGGGGGCT
Tesson_IgM_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
G4
00X-030237063R
TGTAGCAGACCTCCTGTGGTG
Tesson_IgM_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
H3
005-070418635F
GTGACTGCAGAATTCATCTGCCC
Tesson_IgM_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
H4
005-070418635R
GCAGCATATGGGGTTTTCTCACTC
Tesson_IgM_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
A5
009-019047855F
GTGGCTTTGGTCCTCATCTGTC
Tesson_IgM_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
A6
009-019047855R
CCTCTTTCGGAGATAGGCATGTTC
Tesson_IgM_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
B5
018-068645313F
CTGGGAGCCTGGAAATTTGTCTG
Tesson_IgM_Ranking-RVDs_5TC	Warning! Primer pair was generated using suboptimal parameters. 
B6
018-068645313R
GGACACAAGCATATATTATTCTTTGGATGTGCTC
Tesson_IgM_Ranking-RVDs_5TC	Warning! Primer pair was generated using suboptimal parameters. 
C5
004-003843423F
GGTGACCAATACCAACCATCAGG
Tesson_IgM_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
C6
004-003843423R
CCTCCTTTCAGGAAGGCTCTTTCT
Tesson_IgM_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
D5
003-168497270F
ATCTGTCTCCGAGTGCTTCTACG
Tesson_IgM_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. Warning! The sequence of this region of the genome is poorly mapped. 
D6
003-168497270R
CACAGGTGGGTGATTTCGAAAGC
Tesson_IgM_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. Warning! The sequence of this region of the genome is poorly mapped. 
E5
012-031851371F
GGGTGCCTGACTGCTATATTTCC
Tesson_IgM_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
E6
012-031851371R
TTGCCTGCCACAACCATGCATC
Tesson_IgM_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
F5
018-028922784F
TCTCAAGGAGAACCAGCGCG
Tesson_IgM_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
F6
018-028922784R
GGCTTCACCTCACTGACTCG
Tesson_IgM_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
G5
018-072979833F
CCGAGTGAAGGTAGCAGAACTCAG
Tesson_IgM_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
G6
018-072979833R
TTGCTTAGAGGGACCCAGGAAGA
Tesson_IgM_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
H5
017-030652372F
TCCGCTCCTGCACCAGGTTT
Tesson_IgM_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
H6
017-030652372R
GGAGTAAGTCAGCATTGAGTGGG
Tesson_IgM_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
A7
018-019069491F
GGGTTCCTGCCTATAAGCATACCG
Tesson_IgM_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
A8
018-019069491R
GCTAGTGTGTGAAAGACTCCTAGG
Tesson_IgM_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
B7
002-139129605F
CCCATGTGCAGAAGGAGTTGTAC
Tesson_IgM_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. Warning! The sequence of this region of the genome is poorly mapped. 
B8
002-139129605R
GTTCCAGGTCAGTCAGGGATATAC
Tesson_IgM_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. Warning! The sequence of this region of the genome is poorly mapped. 
C7
002-040496981F
GTGTGTGTGTGTGTGTGTGTGG
Tesson_IgM_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
C8
002-040496981R
TCTTCCACACCCTGTGCACC
Tesson_IgM_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
D7
006-052366880F
GGGGGAGGGAACCTTAATAGGA
Tesson_IgM_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
D8
006-052366880R
CAGAAAGTGCTGAGCCAAGTGAC
Tesson_IgM_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
E7
00X-124470518F
CCTTGTGACCACCCCCAAGA
Tesson_IgM_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
E8
00X-124470518R
ACTGTGGGGAAAAGGATCTTGGC
Tesson_IgM_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
F7
004-174994043F
GTGCGGCTCTGTCCTCACAT
Tesson_IgM_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
F8
004-174994043R
GATTAAATTGCATGCAGCTGCACTAACTCC
Tesson_IgM_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
G7
005-121313865F
GATAAACTCATGCTCTGGGGTTGG
Tesson_IgM_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
G8
005-121313865R
GCTCTCCATGGAGGCTAGTG
Tesson_IgM_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
H7
003-024415174F
TTTCCCATCCTGCCATGCCAC
Tesson_IgM_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
H8
003-024415174R
GGCTTACCAATGTACGACTGTGC
Tesson_IgM_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
A9
N/A
Primers Not Generated
A10
N/A
Primers Not Generated!
B9
003-060396362F
CCACTGGGAGTGTATTCGGTATG
Tesson_IgM_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
B10
003-060396362R
GTAAACAGGCCTCTACAGAACGG
Tesson_IgM_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
C9
008-005163674F
AGGAGGAGGAGGAGGAGGAA
Tesson_IgM_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. Warning! The sequence of this region of the genome is poorly mapped. 
C10
008-005163674R
TATATGTGGCTGGAGTCACAGGTC
Tesson_IgM_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. Warning! The sequence of this region of the genome is poorly mapped. 
D9
008-120556664F
CAATTGAATCAGGACCCGGTTTGG
Tesson_IgM_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
D10
008-120556664R
GGTCAGTAATGATGGGTCTCTCC
Tesson_IgM_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
E9
010-049832087F
CCCTGTTCCCACTTTCCTTCTTAG
Tesson_IgM_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
E10
010-049832087R
GCCTTACAGGAAAATGGTGACCTC
Tesson_IgM_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
F9
dom-000053994F
CCCTGTTCCCACTTTCCTTCTTAG
Tesson_IgM_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
F10
dom-000053994R
GCCTTACAGGAAAATGGTGACCTC
Tesson_IgM_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
G9
004-006079924F
GTGTTCTACCCTCAGCCCAACAA
Tesson_IgM_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
G10
004-006079924R
GGGAAGGGCACACTGTTTATTACC
Tesson_IgM_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
H9
015-033211731F
ACCAGGAGGAGCAGGGTGAA
Tesson_IgM_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
H10
015-033211731R
GCCTCACCCTGCCCAGTTAA
Tesson_IgM_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
A11
004-068260584F
CCCGTCTCTGTCAAACATGCTAAG
Tesson_IgM_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
A12
004-068260584R
GGAACTTCATAACGACTGCGGTTG
Tesson_IgM_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
B11
004-068285787F
GAGTGTGCTGAGTCACTCATCTCT
Tesson_IgM_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
B12
004-068285787R
GGAACTTCATAACGACTGCGGTTG
Tesson_IgM_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
C11
005-161756246F
CTCTAGGGGCTAAGTATCTCAGCT
Tesson_IgM_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
C12
005-161756246R
GGTGGCAAAATCTAAACCCTGTGG
Tesson_IgM_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
D11
006-076453434F
GGACCATTGTCAAATCAGGTGGG
Tesson_IgM_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. Warning! The sequence of this region of the genome is poorly mapped. 
D12
006-076453434R
GCCAGGGTGCCATGATTCCTA
Tesson_IgM_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. Warning! The sequence of this region of the genome is poorly mapped. 
E11
019-006170213F
GAGAAAGGTTTGGCAGAACGAGTC
Tesson_IgM_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
E12
019-006170213R
CCAGCTGGCTGGTCCAAATC
Tesson_IgM_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
F11
009-052013154F
GAGGCTGAGCTTGGCTTGCTT
Tesson_IgM_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
F12
009-052013154R
GCAAGGTCACATAGTGTCACTTGG
Tesson_IgM_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
G11
015-073002183F
CCTTGCTCTCCATCCCACAC
Tesson_IgM_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
G12
015-073002183R
GAGTCTTGGGTCTGATTGCTTGG
Tesson_IgM_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
H11
005-170773963F
CCAAGGTTGCTGCCAGTTCAGCT
Tesson_IgM_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
H12
005-170773963R
AGGAGCTGGAGAGCCAAGTG
Tesson_IgM_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
Primer Plate #3
A1
00X-131557987F
TTTTGGATGTCAGCTATAGGGTTGGAGAAAATCTT
Tesson_IgM_Ranking-RVDs_5TC	Warning! Primer pair was generated using suboptimal parameters. 
A2
00X-131557987R
CAACAAATGATGCTAATCTAACTGGATGTCTACATG
Tesson_IgM_Ranking-RVDs_5TC	Warning! Primer pair was generated using suboptimal parameters. 
B1
00X-022942491F
GGCAAGCCAAAGGAGTCTCCTG
Tesson_IgM_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
B2
00X-022942491R
TCTCAGTGCCAGCTCGTCCA
Tesson_IgM_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
C1
00X-040773744F
GGGTGAAACAGCATGTGGCATC
Tesson_IgM_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
C2
00X-040773744R
GGTCCTATTAACTCCTACCTCCAG
Tesson_IgM_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
D1
017-087721857F
CCAAGGATTTCCTGACATCGTGTG
Tesson_IgM_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
D2
017-087721857R
TGTGAGAAACCTGGGTGAGCC
Tesson_IgM_Ranking-RVDs_5TC	Primer pair generated using optimal parameters.