Top Cleavage Sites for Tesson_IgM_Ranking-RVDs

PROGNOS Summary for Tesson_IgM_Ranking-RVDs_5T

The rn4 genome was searched for the TALEN pair targeting:
5'- TTCCTGCCCAGCTCCAT NN..NN ACCAGAACAACACTGAA -3'

Using these RVDs:
Left:  01NG02HD03HD04NG05NN06HD07HD08HD09NI10NN11HD12NG13HD14HD15NI16NG
Right: 01NG02HD03NI04NN05NG06NN07NG08NG09NN10NG11NG12HD13NG14NN15NN16NG

Up to 5 mismatches were allowed in each nuclease half-site.

Allowed spacing distances were: 12 13 14 15 16 17 18 19 20 21 22 23 24

Only heterodimers of the nuclease pair were included in the output

1579 total sites were located
Mismatch Types Genomic Regions
  0 and 0: 1
  3 and 3: 1
  2 and 5: 2
  3 and 4: 6
  3 and 5: 49
  4 and 4: 19
  4 and 5: 322
  5 and 5: 1179
  Exons: 23
  Promoters: 8
  Introns: 404
  Intergenic: 1144


The top 100 sites are ranked below according to a weighting of the RVD base preferences. All sites listed contain flanking 5' thymidines.
For the full output, click here.

This output will be deleted from the server one week after it is generated.  Please save your results.

Primer pairs were designed using the 400 bp of sequence on either side of the cleavage site to melt close to 60 °C

For easy visualization of cleavage on gels:
The smallest fragment will be longer than 130 bp, which is also the minimum separation distance between uncleaved and cleaved products.
The two cleavage products will be separated by a minimum size of 0 bp
To optimize assay conditions, the amplicons have a minimum length of 260 bp, a maximum length of 450 bp, and an optimal length of 270 bp.
Rank: 1	Homology Score: 72	RVD Match Score: 2	Left Half-Site RVD Score: 3.404	Right Half-Site RVD Score: 2.361
	Genomic Region: Intergenic	Closest Gene: Adam6	Caution! This site scores very closely to the intended nuclease target. The potential for cleavage at this site is of great concern.
Match Type: R17L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-ATGAC TTCAGTGTTGTTCTGGT    AGTTCCAGGAGAAGGAA     ATGGAGCTGGGCAGGAA GTCCC-3'
	3'-TACTG AAGTCACAACAAGACCA    TCAAGGTCCTCTTCCTT     TACCTCGACCCGTCCTT CAGGG-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 006-138443970
Forward Primer: 5'-GGAATCGGCACATGCAGATCTTTG-3' T_m: 59.038
Reverse Primer: 5'-TCTGGTCCTCAGAGAGTCAGTC-3' T_m: 59.258
Expected PCR Product Length: 325
Expected CelI Cleavage Product Sizes: 194, 131
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr6:138444137-138444187

Rank: 2	Homology Score: 18	RVD Match Score: 2.668	Left Half-Site RVD Score: 5.677	Right Half-Site RVD Score: 4.477
	Genomic Region: Intron	Closest Gene: Pdcd11
Match Type: R16L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-TTGGA TGCAGACTTGTTCTGGT     CCTGTTAGCAGACCTC     AGGGATCTGGGGAGGAA TTAGC-3'
	3'-AACCT ACGTCTGAACAAGACCA     GGACAATCGTCTGGAG     TCCCTAGACCCCTCCTT AATCG-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 001-252428669
Forward Primer: 5'-GTTGTGTTTCAGGACAGCACTACC-3' T_m: 59.038
Reverse Primer: 5'-GTTGGAGAGGCCACATTCTTAACC-3' T_m: 59.038
Expected PCR Product Length: 308
Expected CelI Cleavage Product Sizes: 162, 146
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr1:252428804-252428853

Rank: 3	Homology Score: 10	RVD Match Score: 2.705	Left Half-Site RVD Score: 3.639	Right Half-Site RVD Score: 7.301
	Genomic Region: Intergenic	Closest Gene: Mir532
Match Type: L21R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-TTTTT TTCCTACCCACCCCCAT  GCCTCCCCAGCCTTGGATGAA   ACAGGAACAAAAGTCAA AATTT-3'
	3'-AAAAA AAGGATGGGTGGGGGTA  CGGAGGGGTCGGAACCTACTT   TGTCCTTGTTTTCAGTT TTAAA-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 00X-027254372
Forward Primer: 5'-CCATTATCGCTAGCCTAGCTTACG-3' T_m: 59.038
Reverse Primer: 5'-GACTGGGAATAAGTGGTGTGGTAC-3' T_m: 59.038
Expected PCR Product Length: 301
Expected CelI Cleavage Product Sizes: 167, 134
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chrX:27254510-27254564

Rank: 4	Homology Score: 17	RVD Match Score: 2.748	Left Half-Site RVD Score: 3.946	Right Half-Site RVD Score: 7.218
	Genomic Region: Intergenic	Closest Gene: Lrfn2
Match Type: L24R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-TCCCC TTCCTGCCCAGCTCCCA TCCCCACCCCCTTCTGAGAACTAA ACAGGAAGCACACGGAA CCTGC-3'
	3'-AGGGG AAGGACGGGTCGAGGGT AGGGGTGGGGGAAGACTCTTGATT TGTCCTTCGTGTGCCTT GGACG-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 009-007190270
Forward Primer: 5'-AGTTGGGCTGGTAGAAATTGCTGG-3' T_m: 59.038
Reverse Primer: 5'-CTGAGTGAAACAAACGGCTCCGA-3' T_m: 59.148
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 133, 137
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr9:7190372-7190429

Rank: 5	Homology Score: 8	RVD Match Score: 2.759	Left Half-Site RVD Score: 5.576	Right Half-Site RVD Score: 5.095
	Genomic Region: Intergenic	Closest Gene: Rps29
Match Type: L16R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-GCACA TTCCTCCACATTTCCAT     CTCGAGGAAAGATTGG     ATCAGATCAAGAGTGAA ACTCA-3'
	3'-CGTGT AAGGAGGTGTAAAGGTA     GAGCTCCTTTCTAACC     TAGTCTAGTTCTCACTT TGAGT-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 006-090557385
Forward Primer: 5'-GTCCCCACAGGCATATGATCG-3' T_m: 59.378
Reverse Primer: 5'-GTGCTTCGGGCAGTAGAGCT-3' T_m: 59.508
Expected PCR Product Length: 348
Expected CelI Cleavage Product Sizes: 217, 131
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr6:90557575-90557624

Rank: 6	Homology Score: 5	RVD Match Score: 2.775	Left Half-Site RVD Score: 6.076	Right Half-Site RVD Score: 4.891
	Genomic Region: Intergenic	Closest Gene: Fyn
Match Type: R14L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-GGAGA TCCAGTTTTCTTCTTGT      CAGGGGTGGGTGGG      ATGGGGTGGGGTAGGTA GGAGA-3'
	3'-CCTCT AGGTCAAAAGAAGAACA      GTCCCCACCCACCC      TACCCCACCCCATCCAT CCTCT-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. Warning! The sequence of this region of the genome is poorly mapped. 
Primer Index: 020-043706993
Forward Primer: 5'-GCAGAAATGGATAGTTTGTGGGGG-3' T_m: 59.038
Reverse Primer: 5'-CCGCCAAGCCCTTTTTAAGTACTG-3' T_m: 59.038
Expected PCR Product Length: 378
Expected CelI Cleavage Product Sizes: 248, 130
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr20:43707215-43707262

Rank: 7	Homology Score: 5	RVD Match Score: 2.786	Left Half-Site RVD Score: 5.664	Right Half-Site RVD Score: 5.212
	Genomic Region: Intron	Closest Gene: Prickle2
Match Type: L13R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-CCTGT TTCCTTCCCAAATCCCC      TGACAAGACATAG       AGCAGAAGAACATTTAA AATTA-3'
	3'-GGACA AAGGAAGGGTTTAGGGG      ACTGTTCTGTATC       TCGTCTTCTTGTAAATT TTAAT-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 004-126653383
Forward Primer: 5'-CCATGTGACCTTTGGACTACATGC-3' T_m: 59.038
Reverse Primer: 5'-GAGAGCCACTGACTACCATAACAC-3' T_m: 59.038
Expected PCR Product Length: 327
Expected CelI Cleavage Product Sizes: 145, 182
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr4:126653503-126653549

Rank: 8	Homology Score: 10	RVD Match Score: 2.794	Left Half-Site RVD Score: 4.36	Right Half-Site RVD Score: 7.012
	Genomic Region: Intron	Closest Gene: Rnf4
Match Type: L19R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-GTAGA TCCCTCCCCAGCTCCCT   TGTCAGGACCTAGCCAGGT    ACATGATCAACATTCAA GAAAA-3'
	3'-CATCT AGGGAGGGGTCGAGGGA   ACAGTCCTGGATCGGTCCA    TGTACTAGTTGTAAGTT CTTTT-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 014-082098705
Forward Primer: 5'-AGCCAAGGAAGAAACCTGTGCAGC-3' T_m: 60.908
Reverse Primer: 5'-CTGTGGATGGCTAGAGTGTACTCA-3' T_m: 59.038
Expected PCR Product Length: 300
Expected CelI Cleavage Product Sizes: 130, 170
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr14:82098807-82098859

Rank: 9	Homology Score: 10	RVD Match Score: 2.794	Left Half-Site RVD Score: 7.385	Right Half-Site RVD Score: 4.124
	Genomic Region: Intergenic	Closest Gene: Pard3
Match Type: R12L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-TTTGC TTTTTTATTGTTCTGGA       AGTTAAGTTTGA       ATGGGGCTTGGTAGGAA ATGAT-3'
	3'-AAACG AAAAAATAACAAGACCT       TCAATTCAAACT       TACCCCGAACCATCCTT TACTA-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 019-057896612
Forward Primer: 5'-CCCAGTGCTGGGGTTACAAACA-3' T_m: 59.258
Reverse Primer: 5'-TCTTAGCCAGGAATGAGGCTGC-3' T_m: 59.258
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 138, 132
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr19:57896725-57896770

Rank: 10	Homology Score: 10	RVD Match Score: 2.802	Left Half-Site RVD Score: 6.686	Right Half-Site RVD Score: 4.635
	Genomic Region: Intergenic	Closest Gene: Pde4b
Match Type: R15L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-TTTCG TCCATTATTGTCATGGT     GTCATGCAAGGTGAC      ATGGTGCTGGGGAGGCA GCTGA-3'
	3'-AAAGC AGGTAATAACAGTACCA     CAGTACGTTCCACTG      TACCACGACCCCTCCGT CGACT-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 005-123636672
Forward Primer: 5'-CTCTGAGTGACACGAATTAGTGGG-3' T_m: 59.038
Reverse Primer: 5'-GGAGTGTGTCACTAAGGCTGG-3' T_m: 59.378
Expected PCR Product Length: 350
Expected CelI Cleavage Product Sizes: 218, 132
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr5:123636864-123636912

Rank: 11	Homology Score: 5	RVD Match Score: 2.803	Left Half-Site RVD Score: 5.862	Right Half-Site RVD Score: 5.251
	Genomic Region: Intergenic	Closest Gene: Tasp1
Match Type: R14L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-TGTCT TTCAGTTTTGTTGTACT      GAGAGAGAAAGAGA      ATGAAGTTGGGTGGGTA GGAGG-3'
	3'-ACAGA AAGTCAAAACAACATGA      CTCTCTCTTTCTCT      TACTTCAACCCACCCAT CCTCC-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 003-128243778
Forward Primer: 5'-CAACCACACTGCAGACCAGG-3' T_m: 59.508
Reverse Primer: 5'-GCCATGGATCTGATATTGGTGGCT-3' T_m: 59.038
Expected PCR Product Length: 308
Expected CelI Cleavage Product Sizes: 131, 177
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr3:128243883-128243930

Rank: 12	Homology Score: 13	RVD Match Score: 2.805	Left Half-Site RVD Score: 6.508	Right Half-Site RVD Score: 4.782
	Genomic Region: Intergenic	Closest Gene: Atpbd4
Match Type: R15L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-CATAA TTCAATGTTGTTTGGGC     TTTTTTTTTTTTTTT      TTGGAGCTGGGGACGAA CCCAG-3'
	3'-GTATT AAGTTACAACAAACCCG     AAAAAAAAAAAAAAA      AACCTCGACCCCTGCTT GGGTC-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 003-100952790
Forward Primer: 5'-GCCATGAAGAAGACATGCAGGAG-3' T_m: 59.148
Reverse Primer: 5'-GGATCACTTGAAATGTGGAGCTTCCTAC-3' T_m: 60.298
Expected PCR Product Length: 405
Expected CelI Cleavage Product Sizes: 218, 187
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr3:100952982-100953030

Rank: 13	Homology Score: 17	RVD Match Score: 2.822	Left Half-Site RVD Score: 5.219	Right Half-Site RVD Score: 5.929
	Genomic Region: Intergenic	Closest Gene: RGD1559903
Match Type: R16L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-TTCCA TGCAGTGTTGTTCTGTT     GTCAGTAATGGATGCC     AGAGAGTTGGGATGGAA TGAGA-3'
	3'-AAGGT ACGTCACAACAAGACAA     CAGTCATTACCTACGG     TCTCTCAACCCTACCTT ACTCT-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 020-016460972
Forward Primer: 5'-GTCAAGGACGAAGTCATCTGGAG-3' T_m: 59.148
Reverse Primer: 5'-GACAAGCTGACTGCTGCTAGAG-3' T_m: 59.258
Expected PCR Product Length: 340
Expected CelI Cleavage Product Sizes: 144, 196
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr20:16461089-16461138

Rank: 14	Homology Score: 13	RVD Match Score: 2.829	Left Half-Site RVD Score: 4.456	Right Half-Site RVD Score: 7.218
	Genomic Region: Intron	Closest Gene: Sars
Match Type: L22R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-GTAAG TACCTCCCCAGCTCAAT  TCCACTCACACAACCACAGATA  ACCAGGCCAAAACAGAA GTACC-3'
	3'-CATTC ATGGAGGGGTCGAGTTA  AGGTGAGTGTGTTGGTGTCTAT  TGGTCCGGTTTTGTCTT CATGG-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 002-204001007
Forward Primer: 5'-AATTTATGGGCTCTGTGCCGTGC-3' T_m: 59.148
Reverse Primer: 5'-CCCAGTGCTAAGTTGAGTGTGGC-3' T_m: 61.088
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 135, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr2:204001112-204001167

Rank: 15	Homology Score: 2	RVD Match Score: 2.834	Left Half-Site RVD Score: 6.029	Right Half-Site RVD Score: 5.34
	Genomic Region: Intron	Closest Gene: Prex1
Match Type: R14L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-GAACC TTAAGTACTATCCTGGT      AGAAGCGGTTTGGG      AGGGGTTTGGGGAGGAA GTCCA-3'
	3'-CTTGG AATTCATGATAGGACCA      TCTTCGCCAAACCC      TCCCCAAACCCCTCCTT CAGGT-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 003-157836620
Forward Primer: 5'-GGCAAAGCTGCCTGTCACCA-3' T_m: 59.508
Reverse Primer: 5'-GCTTCAGGCACAGCTGGATC-3' T_m: 59.508
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 136, 134
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr3:157836730-157836777

Rank: 16	Homology Score: 13	RVD Match Score: 2.836	Left Half-Site RVD Score: 6.116	Right Half-Site RVD Score: 5.124
	Genomic Region: Intron	Closest Gene: Ppm1l
Match Type: L12R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-ACTCT TTCCTCCCAAGCTGCTT       TTGGGCGGTTTT       ACCACAATAACAGTGAA GCAAA-3'
	3'-TGAGA AAGGAGGGTTCGACGAA       AACCCGCCAAAA       TGGTGTTATTGTCACTT CGTTT-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 002-159432579
Forward Primer: 5'-GCTAAATCCCCAACCCTGCAG-3' T_m: 59.378
Reverse Primer: 5'-GTGTTTACTTGCTCAAACACCCGG-3' T_m: 59.038
Expected PCR Product Length: 284
Expected CelI Cleavage Product Sizes: 154, 130
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr2:159432708-159432753

Rank: 17	Homology Score: 10	RVD Match Score: 2.849	Left Half-Site RVD Score: 7.669	Right Half-Site RVD Score: 4.296
	Genomic Region: Intergenic	Closest Gene: Mctp2
Match Type: R14L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-AATCA TTCAGTGAATTTGTTGT      GTCACATCTGCCCC      ATGGAGGTGGGAGGGAA GAATA-3'
	3'-TTAGT AAGTCACTTAAACAACA      CAGTGTAGACGGGG      TACCTCCACCCTCCCTT CTTAT-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 001-127719260
Forward Primer: 5'-CCAACATTCTCCCAAGAGATTCTGGAAAC-3' T_m: 60.178
Reverse Primer: 5'-GTTCCAGGTCAACCAGGACTAC-3' T_m: 59.258
Expected PCR Product Length: 368
Expected CelI Cleavage Product Sizes: 135, 233
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr1:127719369-127719416

Rank: 18	Homology Score: 10	RVD Match Score: 2.853	Left Half-Site RVD Score: 5.066	Right Half-Site RVD Score: 6.57
	Genomic Region: Intron	Closest Gene: Ralgapa1
Match Type: L13R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-TCTCC TTCCTTTCCAGCTCCCT      TCTTTCTTTCATT       ACTAGATATGCACTGAA AGCTG-3'
	3'-AGAGG AAGGAAAGGTCGAGGGA      AGAAAGAAAGTAA       TGATCTATACGTGACTT TCGAC-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 006-075907048
Forward Primer: 5'-GGTGTCCTCATTCACCTCTGACAA-3' T_m: 59.038
Reverse Primer: 5'-GTGTTTGGAACATCCTCTGCGAC-3' T_m: 59.148
Expected PCR Product Length: 363
Expected CelI Cleavage Product Sizes: 189, 174
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr6:75907212-75907258

Rank: 19	Homology Score: 10	RVD Match Score: 2.854	Left Half-Site RVD Score: 6.541	Right Half-Site RVD Score: 5.089
	Genomic Region: Intergenic	Closest Gene: Zfand6
Match Type: R15L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-AGGAG TTCAGTCATGTTCAAGG     GCACTTCTTGTGTAA      ATGGGGCTGGACAGGGA GGAGT-3'
	3'-TCCTC AAGTCAGTACAAGTTCC     CGTGAAGAACACATT      TACCCCGACCTGTCCCT CCTCA-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 001-140972716
Forward Primer: 5'-GGGACCACCTCAGCTAAGGA-3' T_m: 59.508
Reverse Primer: 5'-GTGGGGTTGTTGGTTTTCAGCTC-3' T_m: 59.148
Expected PCR Product Length: 368
Expected CelI Cleavage Product Sizes: 173, 195
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr1:140972863-140972911

Rank: 20	Homology Score: 10	RVD Match Score: 2.865	Left Half-Site RVD Score: 6.046	Right Half-Site RVD Score: 5.448
	Genomic Region: Intergenic	Closest Gene: Fundc1
Match Type: L19R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-AGACC TTCCTAACCCTTTCCAT   TTGCTTAAATTAACAGAGA    AACAGAACAATACTCAA AATTG-3'
	3'-TCTGG AAGGATTGGGAAAGGTA   AACGAATTTAATTGTCTCT    TTGTCTTGTTATGAGTT TTAAC-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 00X-016509931
Forward Primer: 5'-CTACCAATGTCTATGGAGTGACCG-3' T_m: 59.038
Reverse Primer: 5'-GCCTAGAGTATCCCAAATGTGCAC-3' T_m: 59.038
Expected PCR Product Length: 365
Expected CelI Cleavage Product Sizes: 130, 235
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chrX:16510033-16510085

Rank: 21	Homology Score: 5	RVD Match Score: 2.867	Left Half-Site RVD Score: 6.642	Right Half-Site RVD Score: 5.105
	Genomic Region: Intergenic	Closest Gene: Ppp4r2
Match Type: R15L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-TAAAT TTAAATTTTTTTCTGGA     GGGTGATCACAGGGG      TTGGAGTGGGGCAGGCA CAGCA-3'
	3'-ATTTA AATTTAAAAAAAGACCT     CCCACTAGTGTCCCC      AACCTCACCCCGTCCGT GTCGT-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 004-135905706
Forward Primer: 5'-GACAACGACTGCGTTCTATTTGGG-3' T_m: 59.038
Reverse Primer: 5'-TCACGAGCCCTGGTTATTGTTTGG-3' T_m: 59.038
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 134, 136
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr4:135905814-135905862

Rank: 22	Homology Score: 10	RVD Match Score: 2.867	Left Half-Site RVD Score: 7.279	Right Half-Site RVD Score: 4.664
	Genomic Region: Exon	Closest Gene: Lrp1
Match Type: R20L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-GCTGT TTCAATCTTGGGGTGGT   TCCCCCAGTCTGACCAGTAC   ATGGTGCTGGGAAGGGA GAGAT-3'
	3'-CGACA AAGTTAGAACCCCACCA   AGGGGGTCAGACTGGTCATG   TACCACGACCCTTCCCT CTCTA-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 007-067524783
Forward Primer: 5'-TGATCAGAGCCTGCCCCTTC-3' T_m: 59.508
Reverse Primer: 5'-GACAGGGTGCTTCCTCACAATAC-3' T_m: 59.148
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 138, 132
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr7:67524892-67524945

Rank: 23	Homology Score: 10	RVD Match Score: 2.869	Left Half-Site RVD Score: 5.089	Right Half-Site RVD Score: 6.686
	Genomic Region: Intron	Closest Gene: Tsc2
Match Type: L18R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-TTAGC TACCTGTCCAGCACCAT    GTCTGCCTGGTGCCTCCC    ACCATGACAATAATGGA CTAAA-3'
	3'-AATCG ATGGACAGGTCGTGGTA    CAGACGGACCACGGAGGG    TGGTACTGTTATTACCT GATTT-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 010-013862379
Forward Primer: 5'-CATAGGTGGGGCCTTATCAGAG-3' T_m: 59.258
Reverse Primer: 5'-GGTCCCTAGTCAAGGACATTGTCT-3' T_m: 59.038
Expected PCR Product Length: 273
Expected CelI Cleavage Product Sizes: 131, 142
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr10:13862482-13862533

Rank: 24	Homology Score: 8	RVD Match Score: 2.882	Left Half-Site RVD Score: 5.188	Right Half-Site RVD Score: 6.672
	Genomic Region: Intergenic	Closest Gene: Lamp5
Match Type: L24R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-ATACC TTCCACCCCACCTCCTT CCCCTAGATTTCAAGAAGGGGGGA AGCAGAACAAAACCTAA CCTCG-3'
	3'-TATGG AAGGTGGGGTGGAGGAA GGGGATCTAAAGTTCTTCCCCCCT TCGTCTTGTTTTGGATT GGAGC-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 003-124119953
Forward Primer: 5'-CAGTAGCTGCCTTTGACCAGGA-3' T_m: 59.258
Reverse Primer: 5'-CAAACCAGACAATGGCTTCTGCTC-3' T_m: 59.038
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 140, 130
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr3:124120062-124120119

Rank: 25	Homology Score: 10	RVD Match Score: 2.882	Left Half-Site RVD Score: 7.727	Right Half-Site RVD Score: 4.47
	Genomic Region: Intergenic	Closest Gene: LOC679825
Match Type: R16L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-TGAGG TTTACTTTTGCTGTGGT     AAAACATCATGACCAA     AGGGACTTGGGCAGGAA AGAGT-3'
	3'-ACTCC AAATGAAAACGACACCA     TTTTGTAGTACTGGTT     TCCCTGAACCCGTCCTT TCTCA-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 001-001765937
Forward Primer: 5'-TTCTAGCCAAGAGGTCCAAAGGCC-3' T_m: 60.908
Reverse Primer: 5'-TGCATGGCCTCTGCATCAGC-3' T_m: 59.508
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 135, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr1:1766045-1766094

Rank: 26	Homology Score: 5	RVD Match Score: 2.887	Left Half-Site RVD Score: 4.754	Right Half-Site RVD Score: 7.343
	Genomic Region: Intergenic	Closest Gene: Pappa
Match Type: L24R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-TTCTC TACCTCCCCACCCCCAT TGCTATGGTTTTGCTCCCTGCATT TCCAGCACAATACAGTA GTGCG-3'
	3'-AAGAG ATGGAGGGGTGGGGGTA ACGATACCAAAACGAGGGACGTAA AGGTCGTGTTATGTCAT CACGC-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 005-082146486
Forward Primer: 5'-GCCAGAGGCCCTGGTCTTAT-3' T_m: 59.508
Reverse Primer: 5'-CTCCATTCCCTACTACAGAGAGTG-3' T_m: 59.038
Expected PCR Product Length: 278
Expected CelI Cleavage Product Sizes: 137, 141
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr5:82146592-82146649

Rank: 27	Homology Score: 5	RVD Match Score: 2.891	Left Half-Site RVD Score: 4.322	Right Half-Site RVD Score: 8.064
	Genomic Region: Intergenic	Closest Gene: Rnf217
Match Type: L17R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-AAGAA TTCATACCAAACTCCAT    ATCACCCTGTATATGAC     AACATAGCACCACTAAA AGTCC-3'
	3'-TTCTT AAGTATGGTTTGAGGTA    TAGTGGGACATATACTG     TTGTATCGTGGTGATTT TCAGG-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Warning! Primer pair was generated using suboptimal parameters. 
Primer Index: 001-026132578
Forward Primer: 5'-CATTCAGATACTCTGAAGCGAAACATGTTAAGG-3' T_m: 59.758
Reverse Primer: 5'-CCCCTGACCAATACTTTGCAAACG-3' T_m: 59.038
Expected PCR Product Length: 334
Expected CelI Cleavage Product Sizes: 148, 186
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr1:26132699-26132749

Rank: 28	Homology Score: 10	RVD Match Score: 2.893	Left Half-Site RVD Score: 6.629	Right Half-Site RVD Score: 5.049
	Genomic Region: Intergenic	Closest Gene: Mir206
Match Type: L23R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-TTTTA TTAGTGCCCAAATCTAT AGTAGTAATGGTCTAATTTGAGC  TCCAGAATAACTCTGAA TCAAA-3'
	3'-AAAAT AATCACGGGTTTAGATA TCATCATTACCAGATTAAACTCG  AGGTCTTATTGAGACTT AGTTT-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 009-019047855
Forward Primer: 5'-GTGGCTTTGGTCCTCATCTGTC-3' T_m: 59.258
Reverse Primer: 5'-CCTCTTTCGGAGATAGGCATGTTC-3' T_m: 59.038
Expected PCR Product Length: 302
Expected CelI Cleavage Product Sizes: 161, 141
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr9:19047986-19048042

Rank: 29	Homology Score: 5	RVD Match Score: 2.894	Left Half-Site RVD Score: 5.348	Right Half-Site RVD Score: 6.57
	Genomic Region: Intron	Closest Gene: Dcc
Match Type: L20R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-AATTC TTGCTACCCAGCCCAAT   ATGGTATTTATATAAAAATA   ATATGATCAACACTGGA TCTAA-3'
	3'-TTAAG AACGATGGGTCGGGTTA   TACCATAAATATATTTTTAT   TATACTAGTTGTGACCT AGATT-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Warning! Primer pair was generated using suboptimal parameters. 
Primer Index: 018-068645313
Forward Primer: 5'-CTGGGAGCCTGGAAATTTGTCTG-3' T_m: 59.148
Reverse Primer: 5'-GGACACAAGCATATATTATTCTTTGGATGTGCTC-3' T_m: 59.668
Expected PCR Product Length: 439
Expected CelI Cleavage Product Sizes: 177, 262
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr18:68645461-68645514

Rank: 30	Homology Score: 10	RVD Match Score: 2.894	Left Half-Site RVD Score: 6.975	Right Half-Site RVD Score: 5.054
	Genomic Region: Intergenic	Closest Gene: Dpp6
Match Type: R15L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-GTCAG TTCTGTGCTGTGCTCAT     GCCATTGTGGGGAAG      AAGGAGATGGGAAGGAA GGTGC-3'
	3'-CAGTC AAGACACGACACGAGTA     CGGTAACACCCCTTC      TTCCTCTACCCTTCCTT CCACG-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 004-003843423
Forward Primer: 5'-GGTGACCAATACCAACCATCAGG-3' T_m: 59.148
Reverse Primer: 5'-CCTCCTTTCAGGAAGGCTCTTTCT-3' T_m: 59.038
Expected PCR Product Length: 319
Expected CelI Cleavage Product Sizes: 169, 150
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr4:3843566-3843614

Rank: 31	Homology Score: 5	RVD Match Score: 2.895	Left Half-Site RVD Score: 6.452	Right Half-Site RVD Score: 5.446
	Genomic Region: Intergenic	Closest Gene: Tmem132b
Match Type: R20L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-GGACC TTCACTGTGATTCTTTT   GTGCATTCAAAAACATCCAC   TTGGGGCTGGGAAGGTA CCTCG-3'
	3'-CCTGG AAGTGACACTAAGAAAA   CACGTAAGTTTTTGTAGGTG   AACCCCGACCCTTCCAT GGAGC-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 012-031851371
Forward Primer: 5'-GGGTGCCTGACTGCTATATTTCC-3' T_m: 59.148
Reverse Primer: 5'-TTGCCTGCCACAACCATGCATC-3' T_m: 59.258
Expected PCR Product Length: 298
Expected CelI Cleavage Product Sizes: 168, 130
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr12:31851510-31851563

Rank: 32	Homology Score: 2	RVD Match Score: 2.898	Left Half-Site RVD Score: 7.255	Right Half-Site RVD Score: 4.891
	Genomic Region: Intergenic	Closest Gene: Smad2
Match Type: R13L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-CTCTC TTCAGTCATCTGTTGGT      ACTGAGCATGACC       ATGGGGTGGGGTTGGAA GGGTG-3'
	3'-GAGAG AAGTCAGTAGACAACCA      TGACTCGTACTGG       TACCCCACCCCAACCTT CCCAC-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 018-072979833
Forward Primer: 5'-CCGAGTGAAGGTAGCAGAACTCAG-3' T_m: 60.908
Reverse Primer: 5'-TTGCTTAGAGGGACCCAGGAAGA-3' T_m: 59.148
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 135, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr18:72979943-72979989

Rank: 33	Homology Score: 5	RVD Match Score: 2.9	Left Half-Site RVD Score: 6.048	Right Half-Site RVD Score: 5.752
	Genomic Region: Intergenic	Closest Gene: Tfap2a
Match Type: L23R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-ACCCT TTCCTCCCCAACTTGCT TCTTGGTCACGATGTTTTAGCCC  AGCAGTAGAAAACTGAA GCCAC-3'
	3'-TGGGA AAGGAGGGGTTGAACGA AGAACCAGTGCTACAAAATCGGG  TCGTCATCTTTTGACTT CGGTG-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 017-030652372
Forward Primer: 5'-TCCGCTCCTGCACCAGGTTT-3' T_m: 59.508
Reverse Primer: 5'-GGAGTAAGTCAGCATTGAGTGGG-3' T_m: 59.148
Expected PCR Product Length: 370
Expected CelI Cleavage Product Sizes: 130, 240
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr17:30652472-30652528

Rank: 34	Homology Score: 5	RVD Match Score: 2.902	Left Half-Site RVD Score: 6.782	Right Half-Site RVD Score: 5.251
	Genomic Region: Intergenic	Closest Gene: Slc7a11
Match Type: R15L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-TTTGT TTCATTTTTGTTTTTGT     TGTGAGAGACAGAAT      ATGAAGTTGGGTGGGTA ATAAG-3'
	3'-AAACA AAGTAAAAACAAAAACA     ACACTCTCTGTCTTA      TACTTCAACCCACCCAT TATTC-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. Warning! The sequence of this region of the genome is poorly mapped. 
Primer Index: 002-139129605
Forward Primer: 5'-CCCATGTGCAGAAGGAGTTGTAC-3' T_m: 59.148
Reverse Primer: 5'-GTTCCAGGTCAGTCAGGGATATAC-3' T_m: 59.038
Expected PCR Product Length: 358
Expected CelI Cleavage Product Sizes: 175, 183
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr2:139129754-139129802

Rank: 35	Homology Score: 5	RVD Match Score: 2.903	Left Half-Site RVD Score: 6.038	Right Half-Site RVD Score: 5.831
	Genomic Region: Intron	Closest Gene: Pde4d
Match Type: R24L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-CCTAA TTCAGTTTGTTTCTAGT AGAGGGAGGGTGAAGGCGCGAAAG CTGGGGGTGGGGGGGAA GCTGC-3'
	3'-GGATT AAGTCAAACAAAGATCA TCTCCCTCCCACTTCCGCGCTTTC GACCCCCACCCCCCCTT CGACG-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 002-040496981
Forward Primer: 5'-GTGTGTGTGTGTGTGTGTGTGG-3' T_m: 59.258
Reverse Primer: 5'-TCTTCCACACCCTGTGCACC-3' T_m: 59.508
Expected PCR Product Length: 273
Expected CelI Cleavage Product Sizes: 142, 131
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr2:40497092-40497149

Rank: 36	Homology Score: 2	RVD Match Score: 2.903	Left Half-Site RVD Score: 6.688	Right Half-Site RVD Score: 5.325
	Genomic Region: Intergenic	Closest Gene: Ferd3l
Match Type: R18L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-CAATT TTGACTGATGTCCTGAT    CTAGAGGGAGGAAAGAAA    GAGGAGTGGGGTAGGAA ATAGA-3'
	3'-GTTAA AACTGACTACAGGACTA    GATCTCCCTCCTTTCTTT    CTCCTCACCCCATCCTT TATCT-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 006-052366880
Forward Primer: 5'-GGGGGAGGGAACCTTAATAGGA-3' T_m: 59.258
Reverse Primer: 5'-CAGAAAGTGCTGAGCCAAGTGAC-3' T_m: 59.148
Expected PCR Product Length: 434
Expected CelI Cleavage Product Sizes: 300, 134
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr6:52367152-52367203

Rank: 37	Homology Score: 5	RVD Match Score: 2.903	Left Half-Site RVD Score: 5.84	Right Half-Site RVD Score: 6.029
	Genomic Region: Intergenic	Closest Gene: Slc15a5
Match Type: L20R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-CGTTA TTAATGCACAGCCCCAT   AAAAACATTGATTTAGAGGA   ACCTTTATAACATTGAA GTGCA-3'
	3'-GCAAT AATTACGTGTCGGGGTA   TTTTTGTAACTAAATCTCCT   TGGAAATATTGTAACTT CACGT-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 004-174994043
Forward Primer: 5'-GTGCGGCTCTGTCCTCACAT-3' T_m: 59.508
Reverse Primer: 5'-GATTAAATTGCATGCAGCTGCACTAACTCC-3' T_m: 60.058
Expected PCR Product Length: 269
Expected CelI Cleavage Product Sizes: 138, 131
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr4:174994152-174994205

Rank: 38	Homology Score: 10	RVD Match Score: 2.91	Left Half-Site RVD Score: 4.664	Right Half-Site RVD Score: 7.713
	Genomic Region: Intergenic	Closest Gene: Zfp385b
Match Type: L19R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-TTTCC TTCCAGCCCATCTCCAC   TTCTTTATGCCAGCACTGA    TCCACAGAAACACTGTA AACCA-3'
	3'-AAAGG AAGGTCGGGTAGAGGTG   AAGAAATACGGTCGTGACT    AGGTGTCTTTGTGACAT TTGGT-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 003-060396362
Forward Primer: 5'-CCACTGGGAGTGTATTCGGTATG-3' T_m: 59.148
Reverse Primer: 5'-GTAAACAGGCCTCTACAGAACGG-3' T_m: 59.148
Expected PCR Product Length: 362
Expected CelI Cleavage Product Sizes: 230, 132
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr3:60396564-60396616

Rank: 39	Homology Score: 5	RVD Match Score: 2.91	Left Half-Site RVD Score: 5.135	Right Half-Site RVD Score: 7.019
	Genomic Region: Intergenic	Closest Gene: LOC654482
Match Type: L19R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-CTGAG TTCTTCCCCACCTCCAC   CTCTGCCAGAAGGCAATTG    TGAAGAACTACACTTAA GTATT-3'
	3'-GACTC AAGAAGGGGTGGAGGTG   GAGACGGTCTTCCGTTAAC    ACTTCTTGATGTGAATT CATAA-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. Warning! The sequence of this region of the genome is poorly mapped. 
Primer Index: 008-005163674
Forward Primer: 5'-AGGAGGAGGAGGAGGAGGAA-3' T_m: 59.508
Reverse Primer: 5'-TATATGTGGCTGGAGTCACAGGTC-3' T_m: 59.038
Expected PCR Product Length: 264
Expected CelI Cleavage Product Sizes: 132, 132
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr8:5163778-5163830

Rank: 40	Homology Score: 2	RVD Match Score: 2.91	Left Half-Site RVD Score: 5.754	Right Half-Site RVD Score: 6.201
	Genomic Region: Intergenic	Closest Gene: Tgfbr2
Match Type: L13R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-GCGCC TCCCTCCCCAACTACTT      CCCTTTCCTCATC       TCCAGATGAATAATGAA GTCTG-3'
	3'-CGCGG AGGGAGGGGTTGATGAA      GGGAAAGGAGTAG       AGGTCTACTTATTACTT CAGAC-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 008-120556664
Forward Primer: 5'-CAATTGAATCAGGACCCGGTTTGG-3' T_m: 59.038
Reverse Primer: 5'-GGTCAGTAATGATGGGTCTCTCC-3' T_m: 59.148
Expected PCR Product Length: 310
Expected CelI Cleavage Product Sizes: 153, 157
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr8:120556792-120556838

Rank: 41	Homology Score: 5	RVD Match Score: 2.913	Left Half-Site RVD Score: 6.113	Right Half-Site RVD Score: 5.848
	Genomic Region: Intergenic	Closest Gene: Tmub1
Match Type: R23L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-AGCTA TTCAATCTTTTTCTTTT AGGGTTTTTCCCCCTCATCTGTC  AGGGAAATGGGCAGGGA AAAGG-3'
	3'-TCGAT AAGTTAGAAAAAGAAAA TCCCAAAAAGGGGGAGTAGACAG  TCCCTTTACCCGTCCCT TTTCC-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 004-006079924
Forward Primer: 5'-GTGTTCTACCCTCAGCCCAACAA-3' T_m: 59.148
Reverse Primer: 5'-GGGAAGGGCACACTGTTTATTACC-3' T_m: 59.038
Expected PCR Product Length: 316
Expected CelI Cleavage Product Sizes: 142, 174
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr4:6080036-6080092

Rank: 42	Homology Score: 2	RVD Match Score: 2.914	Left Half-Site RVD Score: 7.107	Right Half-Site RVD Score: 5.104
	Genomic Region: Intergenic	Closest Gene: Tas2r138
Match Type: R23L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-ATCTG TTAAGTGTTCTTAGGCT ATCTGGAGTTTCAATCCTCTAAA  ATGTTGTTTGGTAGGAA TTCAA-3'
	3'-TAGAC AATTCACAAGAATCCGA TAGACCTCAAAGTTAGGAGATTT  TACAACAAACCATCCTT AAGTT-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 004-068260584
Forward Primer: 5'-CCCGTCTCTGTCAAACATGCTAAG-3' T_m: 59.038
Reverse Primer: 5'-GGAACTTCATAACGACTGCGGTTG-3' T_m: 59.038
Expected PCR Product Length: 311
Expected CelI Cleavage Product Sizes: 180, 131
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr4:68260734-68260790

Rank: 43	Homology Score: 2	RVD Match Score: 2.914	Left Half-Site RVD Score: 7.107	Right Half-Site RVD Score: 5.104
	Genomic Region: Intergenic	Closest Gene: Tas2r138
Match Type: R23L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-ATCTG TTAAGTGTTCTTAGGCT ATCCGGAGTTTCAATCCTCTAAA  ATGTTGTTTGGTAGGAA TTCAA-3'
	3'-TAGAC AATTCACAAGAATCCGA TAGGCCTCAAAGTTAGGAGATTT  TACAACAAACCATCCTT AAGTT-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 004-068285787
Forward Primer: 5'-GAGTGTGCTGAGTCACTCATCTCT-3' T_m: 59.038
Reverse Primer: 5'-GGAACTTCATAACGACTGCGGTTG-3' T_m: 59.038
Expected PCR Product Length: 288
Expected CelI Cleavage Product Sizes: 157, 131
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr4:68285914-68285970

Rank: 44	Homology Score: 8	RVD Match Score: 2.915	Left Half-Site RVD Score: 5.426	Right Half-Site RVD Score: 6.453
	Genomic Region: Intergenic	Closest Gene: Prdm2
Match Type: R19L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-TACCT TTCAGGGTTGTTCTAAA   AACTAACTGGAGAACACAA    ATGGCGCCTGGCATGAA GTAAC-3'
	3'-ATGGA AAGTCCCAACAAGATTT   TTGATTGACCTCTTGTGTT    TACCGCGGACCGTACTT CATTG-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 005-161756246
Forward Primer: 5'-CTCTAGGGGCTAAGTATCTCAGCT-3' T_m: 59.038
Reverse Primer: 5'-GGTGGCAAAATCTAAACCCTGTGG-3' T_m: 59.038
Expected PCR Product Length: 280
Expected CelI Cleavage Product Sizes: 130, 150
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr5:161756348-161756400

Rank: 45	Homology Score: 2	RVD Match Score: 2.915	Left Half-Site RVD Score: 5.333	Right Half-Site RVD Score: 6.798
	Genomic Region: Intergenic	Closest Gene: Brms1l
Match Type: L24R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-CTCTG TTGCTACCCAACTCCCC ACACCAGCAGTGGTACCTTAAGGG CCCAGCATAACATTGCA AACTC-3'
	3'-GAGAC AACGATGGGTTGAGGGG TGTGGTCGTCACCATGGAATTCCC GGGTCGTATTGTAACGT TTGAG-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. Warning! The sequence of this region of the genome is poorly mapped. 
Primer Index: 006-076453434
Forward Primer: 5'-GGACCATTGTCAAATCAGGTGGG-3' T_m: 59.148
Reverse Primer: 5'-GCCAGGGTGCCATGATTCCTA-3' T_m: 59.378
Expected PCR Product Length: 418
Expected CelI Cleavage Product Sizes: 288, 130
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr6:76453691-76453748

Rank: 46	Homology Score: 8	RVD Match Score: 2.918	Left Half-Site RVD Score: 6.804	Right Half-Site RVD Score: 5.348
	Genomic Region: Intergenic	Closest Gene: Stk17b
Match Type: R21L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-TGGCA TTCCGTTTGGTCCTGGT  TACTCCTGCTGACTCATTAGA   ATTGAGCTGGCTGGGAA GGTAA-3'
	3'-ACCGT AAGGCAAACCAGGACCA  ATGAGGACGACTGAGTAATCT   TAACTCGACCGACCCTT CCATT-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 009-052013154
Forward Primer: 5'-GAGGCTGAGCTTGGCTTGCTT-3' T_m: 59.378
Reverse Primer: 5'-GCAAGGTCACATAGTGTCACTTGG-3' T_m: 59.038
Expected PCR Product Length: 311
Expected CelI Cleavage Product Sizes: 157, 154
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr9:52013282-52013336

Rank: 47	Homology Score: 2	RVD Match Score: 2.92	Left Half-Site RVD Score: 5.284	Right Half-Site RVD Score: 6.62
	Genomic Region: Intergenic	Closest Gene: Pcdh20
Match Type: R13L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-ACATT TTAAATTTTGTTCTAAT      GTTAAAACAATGC       AAGGATCTGGGTATAAA AATAT-3'
	3'-TGTAA AATTTAAAACAAGATTA      CAATTTTGTTACG       TTCCTAGACCCATATTT TTATA-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 015-073002183
Forward Primer: 5'-CCTTGCTCTCCATCCCACAC-3' T_m: 59.508
Reverse Primer: 5'-GAGTCTTGGGTCTGATTGCTTGG-3' T_m: 59.148
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 135, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr15:73002293-73002339

Rank: 48	Homology Score: 2	RVD Match Score: 2.92	Left Half-Site RVD Score: 6.069	Right Half-Site RVD Score: 5.913
	Genomic Region: Intron	Closest Gene: LOC246295
Match Type: L20R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-TGTGC TTTCCACACAGCACCAT   GCAGCCTCAGTCACCAGGCC   TCTAGAACAGCATTGTA ACAGG-3'
	3'-ACACG AAAGGTGTGTCGTGGTA   CGTCGGAGTCAGTGGTCCGG   AGATCTTGTCGTAACAT TGTCC-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 005-170773963
Forward Primer: 5'-CCAAGGTTGCTGCCAGTTCAGCT-3' T_m: 61.088
Reverse Primer: 5'-AGGAGCTGGAGAGCCAAGTG-3' T_m: 59.508
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 135, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr5:170774069-170774122

Rank: 49	Homology Score: 8	RVD Match Score: 2.922	Left Half-Site RVD Score: 6.199	Right Half-Site RVD Score: 5.84
	Genomic Region: Intergenic	Closest Gene: Actrt1
Match Type: R14L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-ATTTA TTAATTGTTGGTCTGAT      TACCTATGTTATTA      ATGGTTCTGTTCAGGAA GTTTT-3'
	3'-TAAAT AATTAACAACCAGACTA      ATGGATACAATAAT      TACCAAGACAAGTCCTT CAAAA-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Warning! Primer pair was generated using suboptimal parameters. 
Primer Index: 00X-131557987
Forward Primer: 5'-TTTTGGATGTCAGCTATAGGGTTGGAGAAAATCTT-3' T_m: 59.578
Reverse Primer: 5'-CAACAAATGATGCTAATCTAACTGGATGTCTACATG-3' T_m: 59.498
Expected PCR Product Length: 337
Expected CelI Cleavage Product Sizes: 131, 206
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chrX:131558092-131558139

Rank: 50	Homology Score: 5	RVD Match Score: 2.924	Left Half-Site RVD Score: 7.522	Right Half-Site RVD Score: 4.884
	Genomic Region: Intergenic	Closest Gene: RGD1561065
Match Type: R24L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-GGATA TGCATTGATGTACTGTT GCTGTGACAAAATTCTATGACCAA AGCGAGTTGGGGAGGAA AGGGT-3'
	3'-CCTAT ACGTAACTACATGACAA CGACACTGTTTTAAGATACTGGTT TCGCTCAACCCCTCCTT TCCCA-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 00X-040773744
Forward Primer: 5'-GGGTGAAACAGCATGTGGCATC-3' T_m: 59.258
Reverse Primer: 5'-GGTCCTATTAACTCCTACCTCCAG-3' T_m: 59.038
Expected PCR Product Length: 441
Expected CelI Cleavage Product Sizes: 310, 131
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chrX:40774023-40774080

Rank: 51	Homology Score: 2	RVD Match Score: 2.924	Left Half-Site RVD Score: 6.902	Right Half-Site RVD Score: 5.32
	Genomic Region: Intron	Closest Gene: Cubn
Match Type: R17L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-AAATA TTCACAGTTTTTCAGGA    TGATCAATGAAACAACA     ATGGGGTTTGGGGGGAA TACTG-3'
	3'-TTTAT AAGTGTCAAAAAGTCCT    ACTAGTTACTTTGTTGT     TACCCCAAACCCCCCTT ATGAC-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 017-087721857
Forward Primer: 5'-CCAAGGATTTCCTGACATCGTGTG-3' T_m: 59.038
Reverse Primer: 5'-TGTGAGAAACCTGGGTGAGCC-3' T_m: 59.378
Expected PCR Product Length: 273
Expected CelI Cleavage Product Sizes: 142, 131
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr17:87721972-87722022

Rank: 52	Homology Score: 10	RVD Match Score: 2.927	Left Half-Site RVD Score: 7.316	Right Half-Site RVD Score: 5.045
	Genomic Region: Intergenic	Closest Gene: Mir3589
Match Type: R22L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-CTTTG TAGACTGTGGTTCTGCT  GATTGTGGGATGACAGAAAGGA  ATGGAGATGGGCTAGAA GTCCA-3'
	3'-GAAAC ATCTGACACCAAGACGA  CTAACACCCTACTGTCTTTCCT  TACCTCTACCCGATCTT CAGGT-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 00X-146103263
Forward Primer: 5'-CTACACTCCTTTTTGTTGGGTGGG-3' T_m: 59.038
Reverse Primer: 5'-CTCTGCTGCAGAGCCTTTTTTCC-3' T_m: 59.148
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 136, 134
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chrX:146103369-146103424

Rank: 53	Homology Score: 5	RVD Match Score: 2.927	Left Half-Site RVD Score: 5.503	Right Half-Site RVD Score: 6.686
	Genomic Region: Intron	Closest Gene: Grk4
Match Type: L23R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-TCAAC TACCTCTCCAGCACCAT GCCTGCCTGCCTGCCTGCCTGAA  ACCATGACAATAATGGA CTAAA-3'
	3'-AGTTG ATGGAGAGGTCGTGGTA CGGACGGACGGACGGACGGACTT  TGGTACTGTTATTACCT GATTT-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 014-081690069
Forward Primer: 5'-GTGTCACTGAGGGTGTGCTTTG-3' T_m: 59.258
Reverse Primer: 5'-GCTAGCTGTGTGGCATTAAGGAAG-3' T_m: 59.038
Expected PCR Product Length: 327
Expected CelI Cleavage Product Sizes: 130, 197
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr14:81690169-81690225

Rank: 54	Homology Score: 5	RVD Match Score: 2.927	Left Half-Site RVD Score: 6.686	Right Half-Site RVD Score: 5.503
	Genomic Region: Intergenic	Closest Gene: Mir135a
Match Type: R19L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-TTAAG TTCATAATTGTCATGGT   GGGAGGCACGTAGACAGAC    ATGGTGCTGGAGAGGGA GCTGA-3'
	3'-AATTC AAGTATTAACAGTACCA   CCCTCCGTGCATCTGTCTG    TACCACGACCTCTCCCT CGACT-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 007-029017126
Forward Primer: 5'-CTTAGCTGGGGTATTACTGCTGTG-3' T_m: 59.038
Reverse Primer: 5'-AAACGCTTCACTGGGGGTGG-3' T_m: 59.508
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 130, 140
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr7:29017228-29017280

Rank: 55	Homology Score: 8	RVD Match Score: 2.929	Left Half-Site RVD Score: 6.27	Right Half-Site RVD Score: 5.759
	Genomic Region: Intergenic	Closest Gene: Clec14a
Match Type: L17R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-AGGTC TTGCTGCCCATCTTCAA    TAAGCCAAGAGAAAAGA     ACCAGCTTAACAGTGAA AAGTA-3'
	3'-TCCAG AACGACGGGTAGAAGTT    ATTCGGTTCTCTTTTCT     TGGTCGAATTGTCACTT TTCAT-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 006-079162173
Forward Primer: 5'-ACACAGAAGGCTCTCCAGAGAC-3' T_m: 59.258
Reverse Primer: 5'-ACTTTGATCTCACTTCAGGTCCCC-3' T_m: 59.038
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 138, 132
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr6:79162284-79162334

Rank: 56	Homology Score: 2	RVD Match Score: 2.93	Left Half-Site RVD Score: 5.959	Right Half-Site RVD Score: 6.135
	Genomic Region: Intergenic	Closest Gene: Gtpbp4
Match Type: L22R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-TTCCC TTCCTCCCTCCCTCCCT  CCCTCCCTCCCTTCCCCCCTTC  CTGAGAACAATACTGCA ATCTG-3'
	3'-AAGGG AAGGAGGGAGGGAGGGA  GGGAGGGAGGGAAGGGGGGAAG  GACTCTTGTTATGACGT TAGAC-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 017-072075714
Forward Primer: 5'-GCCTGTCACAGTTTGTCTCTTGC-3' T_m: 59.148
Reverse Primer: 5'-GCAGGCTTTTGCATGTTGTCTGAG-3' T_m: 59.038
Expected PCR Product Length: 385
Expected CelI Cleavage Product Sizes: 153, 232
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr17:72075837-72075892

Rank: 57	Homology Score: 5	RVD Match Score: 2.931	Left Half-Site RVD Score: 7.307	Right Half-Site RVD Score: 5.078
	Genomic Region: Intergenic	Closest Gene: Rpa1
Match Type: R13L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-GGATC TTCATTGTCATTGTGGG      GCTGTCATATAGG       GTGGGGGTGGGAAGGAA GCCAT-3'
	3'-CCTAG AAGTAACAGTAACACCC      CGACAGTATATCC       CACCCCCACCCTTCCTT CGGTA-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 010-062612960
Forward Primer: 5'-GAGCTGGCACTTCATGAAGGTC-3' T_m: 59.258
Reverse Primer: 5'-GCCCGTGGACTACAGAGACT-3' T_m: 59.508
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 135, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr10:62613070-62613116

Rank: 58	Homology Score: 2	RVD Match Score: 2.932	Left Half-Site RVD Score: 6.048	Right Half-Site RVD Score: 6.043
	Genomic Region: Intergenic	Closest Gene: Hibch
Match Type: L16R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-ATACT TTCCTCCCCAACTTGCT     TGGACCATAAGGTTTC     ATCATAACAATAGTAAA CCTAA-3'
	3'-TATGA AAGGAGGGGTTGAACGA     ACCTGGTATTCCAAAG     TAGTATTGTTATCATTT GGATT-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 009-045551124
Forward Primer: 5'-AAGCAGCACCCCTCCAAGCA-3' T_m: 59.508
Reverse Primer: 5'-GTGGGCAAAGCAGAAGTAAGCTC-3' T_m: 59.148
Expected PCR Product Length: 359
Expected CelI Cleavage Product Sizes: 140, 219
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr9:45551237-45551286

Rank: 59	Homology Score: 5	RVD Match Score: 2.933	Left Half-Site RVD Score: 5.715	Right Half-Site RVD Score: 6.46
	Genomic Region: Intergenic	Closest Gene: Ddx18
Match Type: L14R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-TGGTC TTCCTACCCCAGTTCAT      CCTGTCATGATAAT      GCCAGAGGCACACTGAA GGGTT-3'
	3'-ACCAG AAGGATGGGGTCAAGTA      GGACAGTACTATTA      CGGTCTCCGTGTGACTT CCCAA-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 013-033948884
Forward Primer: 5'-GAGGCAGCCATCCACATTGTATC-3' T_m: 59.148
Reverse Primer: 5'-CCTTCAGTCGGTGTGGTAGAAAAC-3' T_m: 59.038
Expected PCR Product Length: 344
Expected CelI Cleavage Product Sizes: 157, 187
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr13:33949015-33949062

Rank: 60	Homology Score: 13	RVD Match Score: 2.933	Left Half-Site RVD Score: 5.127	Right Half-Site RVD Score: 7.262
	Genomic Region: Intergenic	Closest Gene: Ifnar1
Match Type: L15R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-CCTTG TTCCTTCCCAGCTGCCT     AGGAATGTGGGACCA      ACCTGAGCAACACAGCA AGAGC-3'
	3'-GGAAC AAGGAAGGGTCGACGGA     TCCTTACACCCTGGT      TGGACTCGTTGTGTCGT TCTCG-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 011-031478392
Forward Primer: 5'-GATCTGATCCCCTTTTCTGGTGTG-3' T_m: 59.038
Reverse Primer: 5'-CTTCTTTAGCACGTCGCCCC-3' T_m: 59.508
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 134, 136
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr11:31478500-31478548

Rank: 61	Homology Score: 5	RVD Match Score: 2.934	Left Half-Site RVD Score: 6.503	Right Half-Site RVD Score: 5.695
	Genomic Region: Intergenic	Closest Gene: Cpa6
Match Type: R24L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-AGGAC TTCAGGATCATTCTGGG CAACCTAAGACTGTTCTGAAAACC AGGCAGCTGGCTAGGAA GATGG-3'
	3'-TCCTG AAGTCCTAGTAAGACCC GTTGGATTCTGACAAGACTTTTGG TCCGTCGACCGATCCTT CTACC-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 005-008485774
Forward Primer: 5'-CACACACACACACACACACACAC-3' T_m: 59.148
Reverse Primer: 5'-CTGCCTCCTAATATGGCCACG-3' T_m: 59.378
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 137, 133
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr5:8485880-8485937

Rank: 62	Homology Score: 2	RVD Match Score: 2.936	Left Half-Site RVD Score: 6.36	Right Half-Site RVD Score: 5.818
	Genomic Region: Exon	Closest Gene: Lrba
Match Type: R21L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-TTGCC TTTTGTCTTATTCTAGT  ACAACCTTGCTGGCTTTTATC   ATGGAGTTGTTGAAGAA CTCAG-3'
	3'-AACGG AAAACAGAATAAGATCA  TGTTGGAACGACCGAAAATAG   TACCTCAACAACTTCTT GAGTC-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 002-178282654
Forward Primer: 5'-GAGCTACATTTCTGCCCCGAAG-3' T_m: 59.258
Reverse Primer: 5'-CCACGCAGACAAATAAGCACACG-3' T_m: 59.148
Expected PCR Product Length: 300
Expected CelI Cleavage Product Sizes: 136, 164
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr2:178282761-178282815

Rank: 63	Homology Score: 2	RVD Match Score: 2.937	Left Half-Site RVD Score: 5.952	Right Half-Site RVD Score: 6.206
	Genomic Region: Intergenic	Closest Gene: Clint1
Match Type: L20R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-CTCAT TCCCTACCCAGTACCAA   GATAGTAACTTTAGAGGTTG   AATAAAATAAAACTGAA TACTG-3'
	3'-GAGTA AGGGATGGGTCATGGTT   CTATCATTGAAATCTCCAAC   TTATTTTATTTTGACTT ATGAC-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 010-030648298
Forward Primer: 5'-CCCACCAGTCCAAATGGTGGTA-3' T_m: 59.258
Reverse Primer: 5'-TGCAAAGCTGCTTGCTCTGGC-3' T_m: 59.378
Expected PCR Product Length: 283
Expected CelI Cleavage Product Sizes: 153, 130
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr10:30648422-30648475

Rank: 64	Homology Score: 8	RVD Match Score: 2.938	Left Half-Site RVD Score: 5.938	Right Half-Site RVD Score: 6.23
	Genomic Region: Intergenic	Closest Gene: Rgs10
Match Type: L22R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-AATTT TCTCTGCCCACCTCCGT  TGAAAGCATCCCCCCAAGGCTG  ATCACAAATACACTGAA GCTAA-3'
	3'-TTAAA AGAGACGGGTGGAGGCA  ACTTTCGTAGGGGGGTTCCGAC  TAGTGTTTATGTGACTT CGATT-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 001-187665749
Forward Primer: 5'-GGAGGGAAGGAGGGAAGGAA-3' T_m: 59.508
Reverse Primer: 5'-ACACCAGTCTTGCTGAGGGC-3' T_m: 59.508
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 132, 138
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr1:187665851-187665906

Rank: 65	Homology Score: 10	RVD Match Score: 2.938	Left Half-Site RVD Score: 4.831	Right Half-Site RVD Score: 7.749
	Genomic Region: Intron	Closest Gene: Fra10ac1
Match Type: L22R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-TCAAG TTCCTAGCCAGCTTCAT  TCACAGAGCAAGTTCCAGGACA  GCCAGGGCTACACAGAA AAACT-3'
	3'-AGTTC AAGGATCGGTCGAAGTA  AGTGTCTCGTTCAAGGTCCTGT  CGGTCCCGATGTGTCTT TTTGA-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 001-242536006
Forward Primer: 5'-TGGAGGTTGGGAGAGTAGAGG-3' T_m: 59.378
Reverse Primer: 5'-TGAAGGAGGGAACACTCAGCAG-3' T_m: 59.258
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 135, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr1:242536111-242536166

Rank: 66	Homology Score: 2	RVD Match Score: 2.941	Left Half-Site RVD Score: 7.226	Right Half-Site RVD Score: 5.203
	Genomic Region: Intergenic	Closest Gene: Nmur2
Match Type: R22L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-TTCTT TTCAGTGACTTTCTGTC  TAGGAGGTCACTTCTTTTAGAT  GTGGGGGTGGGTAGGGA TTGTC-3'
	3'-AAGAA AAGTCACTGAAAGACAG  ATCCTCCAGTGAAGAAAATCTA  CACCCCCACCCATCCCT AACAG-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. Warning! The sequence of this region of the genome is poorly mapped. 
Primer Index: 010-041837672
Forward Primer: 5'-CCCACACTCCTGCTTCTGTATC-3' T_m: 59.258
Reverse Primer: 5'-GTCCATGCTGTCACCAGACATC-3' T_m: 59.258
Expected PCR Product Length: 316
Expected CelI Cleavage Product Sizes: 144, 172
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr10:41837786-41837841

Rank: 67	Homology Score: 2	RVD Match Score: 2.941	Left Half-Site RVD Score: 5.284	Right Half-Site RVD Score: 6.789
	Genomic Region: Intergenic	Closest Gene: Npsr1
Match Type: R13L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-CTGTC TTCAGTATTATTATTAT      TTTTTTTTTTTTT       TTGGAGCTGGGGACCGA ACCCA-3'
	3'-GACAG AAGTCATAATAATAATA      AAAAAAAAAAAAA       AACCTCGACCCCTGGCT TGGGT-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 008-023607288
Forward Primer: 5'-CGGAAGCCATGTTCAGGTCTTCA-3' T_m: 59.148
Reverse Primer: 5'-CAGACCAGAAGACTGTGTGATTGC-3' T_m: 59.038
Expected PCR Product Length: 386
Expected CelI Cleavage Product Sizes: 132, 254
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr8:23607395-23607441

Rank: 68	Homology Score: 2	RVD Match Score: 2.942	Left Half-Site RVD Score: 6.25	Right Half-Site RVD Score: 5.952
	Genomic Region: Intergenic	Closest Gene: Dab2
Match Type: R24L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-GAAGT TTTAGTGTTTCTCTAAT ATTTATGAAGAACCTATGTGTTGT GTGATGCTGGGTTGGAA ATAAA-3'
	3'-CTTCA AAATCACAAAGAGATTA TAAATACTTCTTGGATACACAACA CACTACGACCCAACCTT TATTT-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 002-055689862
Forward Primer: 5'-CTGAGTTTGAGGCCAGTATGGG-3' T_m: 59.258
Reverse Primer: 5'-GCTGCCATTCCACTGAAGGTTG-3' T_m: 59.258
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 138, 132
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr2:55689969-55690026

Rank: 69	Homology Score: 2	RVD Match Score: 2.943	Left Half-Site RVD Score: 6.347	Right Half-Site RVD Score: 5.879
	Genomic Region: Intergenic	Closest Gene: Tcf7l1
Match Type: R23L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-TCCAG TTCTGTGCTATACTAGT TTAAATCGGGGGTCTGGGCGTCC  ATGGATGTTGGTAGCAA CAGGG-3'
	3'-AGGTC AAGACACGATATGATCA AATTTAGCCCCCAGACCCGCAGG  TACCTACAACCATCGTT GTCCC-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 004-106165776
Forward Primer: 5'-ATGCTTAGGCTACTGTGTCTGGC-3' T_m: 59.148
Reverse Primer: 5'-TAGCAGGGTCATGTGGTACAGC-3' T_m: 59.258
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 135, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr4:106165881-106165937

Rank: 70	Homology Score: 5	RVD Match Score: 2.945	Left Half-Site RVD Score: 7.769	Right Half-Site RVD Score: 4.862
	Genomic Region: Intergenic	Closest Gene: Tesl
Match Type: R24L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-TCCTA TTTAGTGTTGCTATTGC TGTGGTAAAACACTACAACCAACC ATTGGGTTGGGAAGGAA CGGGT-3'
	3'-AGGAT AAATCACAACGATAACG ACACCATTTTGTGATGTTGGTTGG TAACCCAACCCTTCCTT GCCCA-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 00X-010426535
Forward Primer: 5'-GTTCTCTGCATCCATGCCATGTG-3' T_m: 59.148
Reverse Primer: 5'-CCTCAGCATCAGTTACTGCTTCC-3' T_m: 59.148
Expected PCR Product Length: 383
Expected CelI Cleavage Product Sizes: 252, 131
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chrX:10426756-10426813

Rank: 71	Homology Score: 8	RVD Match Score: 2.948	Left Half-Site RVD Score: 6.347	Right Half-Site RVD Score: 5.915
	Genomic Region: Intergenic	Closest Gene: Far1
Match Type: R13L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-CTGTC TTAATTGTTGTTGTAGT      ACGGAGACTAACC       CTGAATGTGGGCAGGAA TATTC-3'
	3'-GACAG AATTAACAACAACATCA      TGCCTCTGATTGG       GACTTACACCCGTCCTT ATAAG-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 001-171517384
Forward Primer: 5'-CACCTTGTGTTTCCAGAACTCTGC-3' T_m: 59.038
Reverse Primer: 5'-CTAGTCCTATCACATCCACGGTC-3' T_m: 59.148
Expected PCR Product Length: 292
Expected CelI Cleavage Product Sizes: 157, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr1:171517516-171517562

Rank: 72	Homology Score: 2	RVD Match Score: 2.95	Left Half-Site RVD Score: 5.694	Right Half-Site RVD Score: 6.651
	Genomic Region: Intron	Closest Gene: Ldb2
Match Type: L24R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-GTATG TATGTACCCAACTCCAT CTTTAAAATTATCTTTCGATTAAT ACTACAATCACTCTGAA ACACC-3'
	3'-CATAC ATACATGGGTTGAGGTA GAAATTTTAATAGAAAGCTAATTA TGATGTTAGTGAGACTT TGTGG-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 014-071610248
Forward Primer: 5'-GCTCCAGAAAGTGCCTTGTTCTTG-3' T_m: 59.038
Reverse Primer: 5'-GCCTTTGCCCGCATGGTTTC-3' T_m: 59.508
Expected PCR Product Length: 415
Expected CelI Cleavage Product Sizes: 153, 262
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr14:71610370-71610427

Rank: 73	Homology Score: 5	RVD Match Score: 2.951	Left Half-Site RVD Score: 5.86	Right Half-Site RVD Score: 6.444
	Genomic Region: Intron	Closest Gene: Scn3a
Match Type: L19R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-GGCAC TTCCCTCCCACCACCAC   CAATCATACACCTTACTCC    AACTGGACAAAACTGAA ACAAA-3'
	3'-CCGTG AAGGGAGGGTGGTGGTG   GTTAGTATGTGGAATGAGG    TTGACCTGTTTTGACTT TGTTT-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 003-048025715
Forward Primer: 5'-ACTGTCACACTGCTGTCACGG-3' T_m: 59.378
Reverse Primer: 5'-GACTGTCTTCGCAATAGGGTTATCTCATC-3' T_m: 60.178
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 131, 139
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr3:48025818-48025870

Rank: 74	Homology Score: 2	RVD Match Score: 2.951	Left Half-Site RVD Score: 6.362	Right Half-Site RVD Score: 5.929
	Genomic Region: Intergenic	Closest Gene: Hunk
Match Type: R18L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-GCGGC TACAGTATTGTTATGCC    AGTGTCTCCAAGGAGTGA    AGAGGGTTGGGAAGGAA TGCTC-3'
	3'-CGCCG ATGTCATAACAATACGG    TCACAGAGGTTCCTCACT    TCTCCCAACCCTTCCTT ACGAG-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 011-029469068
Forward Primer: 5'-CTATTTCCGTGTCTTTGGGGATGC-3' T_m: 59.038
Reverse Primer: 5'-GCTTTCCACGTCTATGTCCTGGT-3' T_m: 59.148
Expected PCR Product Length: 376
Expected CelI Cleavage Product Sizes: 206, 170
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr11:29469246-29469297

Rank: 75	Homology Score: 2	RVD Match Score: 2.951	Left Half-Site RVD Score: 5.929	Right Half-Site RVD Score: 6.362
	Genomic Region: Intergenic	Closest Gene: Tiam1
Match Type: L18R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-GAGCA TTCCTTCCCAACCCTCT    TCACTCCTTGGAGACACT    GGCATAACAATACTGTA GCCGC-3'
	3'-CTCGT AAGGAAGGGTTGGGAGA    AGTGAGGAACCTCTGTGA    CCGTATTGTTATGACAT CGGCG-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 011-030381700
Forward Primer: 5'-GCTTTCCACGTCTATGTCCTGGT-3' T_m: 59.148
Reverse Primer: 5'-CTATTTCCGTGTCTTTGGGGATGC-3' T_m: 59.038
Expected PCR Product Length: 376
Expected CelI Cleavage Product Sizes: 174, 202
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr11:30381846-30381897

Rank: 76	Homology Score: 2	RVD Match Score: 2.951	Left Half-Site RVD Score: 6.362	Right Half-Site RVD Score: 5.929
	Genomic Region: Intergenic	Closest Gene: Tiam1
Match Type: R18L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-GCGGC TACAGTATTGTTATGCC    AGTGTCTCCAAGGAGTGA    AGAGGGTTGGGAAGGAA TGCTC-3'
	3'-CGCCG ATGTCATAACAATACGG    TCACAGAGGTTCCTCACT    TCTCCCAACCCTTCCTT ACGAG-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 011-030396776
Forward Primer: 5'-CTATTTCCGTGTCTTTGGGGATGC-3' T_m: 59.038
Reverse Primer: 5'-GCTTTCCACGTCTATGTCCTGGT-3' T_m: 59.148
Expected PCR Product Length: 376
Expected CelI Cleavage Product Sizes: 206, 170
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr11:30396954-30397005

Rank: 77	Homology Score: 10	RVD Match Score: 2.954	Left Half-Site RVD Score: 4.681	Right Half-Site RVD Score: 7.495
	Genomic Region: Intergenic	Closest Gene: Tbl1xr1
Match Type: R22L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-ACATC TCCAGTCTTATTCTGGT  CTATTATCAACAGTAAGGGAAA  ATGAAGCTGCCATGGAA AGTAC-3'
	3'-TGTAG AGGTCAGAATAAGACCA  GATAATAGTTGTCATTCCCTTT  TACTTCGACGGTACCTT TCATG-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 002-106965669
Forward Primer: 5'-ATAGTGGCCAGTGCCTGGAC-3' T_m: 59.508
Reverse Primer: 5'-CCAGGAGAAAAGGCCATCTTCCA-3' T_m: 59.148
Expected PCR Product Length: 307
Expected CelI Cleavage Product Sizes: 131, 176
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr2:106965770-106965825

Rank: 78	Homology Score: 10	RVD Match Score: 2.956	Left Half-Site RVD Score: 7.52	Right Half-Site RVD Score: 5.106
	Genomic Region: Intron	Closest Gene: Tm6sf1
Match Type: R13L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-CTTTG TTCAGTTTTTTTTTTTT      TTTTTTTCTTTTT       TTGGAGCTGGGAACGAA CCCAG-3'
	3'-GAAAC AAGTCAAAAAAAAAAAA      AAAAAAAGAAAAA       AACCTCGACCCTTGCTT GGGTC-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. Warning! The sequence of this region of the genome is poorly mapped. 
Primer Index: 001-138061468
Forward Primer: 5'-CCGTGAAGCAGGTCACAAAGC-3' T_m: 59.378
Reverse Primer: 5'-CAGCAAGGGAAGTGCTACTTGATG-3' T_m: 59.038
Expected PCR Product Length: 303
Expected CelI Cleavage Product Sizes: 164, 139
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr1:138061607-138061653

Rank: 79	Homology Score: 10	RVD Match Score: 2.957	Left Half-Site RVD Score: 5.089	Right Half-Site RVD Score: 7.55
	Genomic Region: Intron	Closest Gene: Emid1
Match Type: L15R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-AACAT TCCCTGTCCAGCTCCAC     CTCTTGGAAACCTTG      AGGAAAACAACCCTGCA GACTT-3'
	3'-TTGTA AGGGACAGGTCGAGGTG     GAGAACCTTTGGAAC      TCCTTTTGTTGGGACGT CTGAA-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 014-085768096
Forward Primer: 5'-GGGAATGGTTAGCCCCATCTC-3' T_m: 59.378
Reverse Primer: 5'-CATGAGGAAACAGTTCAGGAGGG-3' T_m: 59.148
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 136, 134
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr14:85768206-85768254

Rank: 80	Homology Score: 5	RVD Match Score: 2.957	Left Half-Site RVD Score: 5.759	Right Half-Site RVD Score: 6.484
	Genomic Region: Intron	Closest Gene: Ddx31
Match Type: R19L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-GCTAC TTCACTGTGGTTCCAGT   CCAGCTCCACAGTGTCAGC    AGGGAGCTTGGGAGCTA CAGAG-3'
	3'-CGATG AAGTGACACCAAGGTCA   GGTCGAGGTGTCACAGTCG    TCCCTCGAACCCTCGAT GTCTC-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 003-007890866
Forward Primer: 5'-CAGTCTGTTGTTAAGGGATCGAGC-3' T_m: 59.038
Reverse Primer: 5'-GACACTGAGACTTGCATAGGCC-3' T_m: 59.258
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 135, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr3:7890973-7891025

Rank: 81	Homology Score: 5	RVD Match Score: 2.958	Left Half-Site RVD Score: 5.503	Right Half-Site RVD Score: 6.982
	Genomic Region: Intron	Closest Gene: Commd1
Match Type: L15R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-TCGGC TACCTCTCCAGCACCAT     GTCTGCCTACATCCC      ACCATACTAACAATGGA CTAAA-3'
	3'-AGCCG ATGGAGAGGTCGTGGTA     CAGACGGATGTAGGG      TGGTATGATTGTTACCT GATTT-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 014-103592966
Forward Primer: 5'-TACAGGAGGAGTGGCCTTGTG-3' T_m: 59.378
Reverse Primer: 5'-GCGTCAAGGTAAAATGACCAAGGG-3' T_m: 59.038
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 133, 137
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr14:103593073-103593121

Rank: 82	Homology Score: 10	RVD Match Score: 2.959	Left Half-Site RVD Score: 5.049	Right Half-Site RVD Score: 7.161
	Genomic Region: Intergenic	Closest Gene: MGC125002
Match Type: R23L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-TTTGA TCCAGAATTGTTCTGGT CTAAAAGAAATGTGGTGATAAAA  ATGGAGCAGAGATTGAA GGAAA-3'
	3'-AAACT AGGTCTTAACAAGACCA GATTTTCTTTACACCACTATTTT  TACCTCGTCTCTAACTT CCTTT-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 005-113632907
Forward Primer: 5'-GGAGGAATCTCTAGAAAGTCCCAG-3' T_m: 59.038
Reverse Primer: 5'-GGCTCCTATCTGCAGGTCCA-3' T_m: 59.508
Expected PCR Product Length: 339
Expected CelI Cleavage Product Sizes: 208, 131
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr5:113633085-113633141

Rank: 83	Homology Score: 5	RVD Match Score: 2.959	Left Half-Site RVD Score: 6.362	Right Half-Site RVD Score: 5.988
	Genomic Region: Intergenic	Closest Gene: Nck2
Match Type: R23L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-CTTGG TTCACTGACATTATGGT GGGGAGCTTGGCAGCAGGCAGAC  ATGGTTCTGGGCAAGTA GCTGA-3'
	3'-GAACC AAGTGACTGTAATACCA CCCCTCGAACCGTCGTCCGTCTG  TACCAAGACCCGTTCAT CGACT-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 009-042648255
Forward Primer: 5'-CCAGCCCCAACTAAATATTTGCCC-3' T_m: 59.038
Reverse Primer: 5'-GAGGAGACTGGGAGTAAGCTTTG-3' T_m: 59.148
Expected PCR Product Length: 391
Expected CelI Cleavage Product Sizes: 259, 132
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr9:42648484-42648540

Rank: 84	Homology Score: 5	RVD Match Score: 2.96	Left Half-Site RVD Score: 6.85	Right Half-Site RVD Score: 5.615
	Genomic Region: Intron	Closest Gene: Cdon
Match Type: R21L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-TGTGA TTCTGTGTTCTTCTGTG  AGCTCAGGTAAGAGTGGAATG   ATGGGGTTGGGGCTGAA GCCTT-3'
	3'-ACACT AAGACACAAGAAGACAC  TCGAGTCCATTCTCACCTTAC   TACCCCAACCCCGACTT CGGAA-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 008-035238872
Forward Primer: 5'-CTGAGGCCTGAGCTTTCTCG-3' T_m: 59.508
Reverse Primer: 5'-GTGTGCCACTATGCCAGACC-3' T_m: 59.508
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 134, 136
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr8:35238977-35239031

Rank: 85	Homology Score: 2	RVD Match Score: 2.962	Left Half-Site RVD Score: 5.985	Right Half-Site RVD Score: 6.323
	Genomic Region: Intergenic	Closest Gene: LOC686032
Match Type: R13L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-CTACT TTCAATGTTTTTAAAGT      GAGACTGAAAAAG       AGGGAGATGGCGAGGGA GACAG-3'
	3'-GATGA AAGTTACAAAAATTTCA      CTCTGACTTTTTC       TCCCTCTACCGCTCCCT CTGTC-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 001-020261296
Forward Primer: 5'-AAGGGATGCCTCAGCATGGG-3' T_m: 59.508
Reverse Primer: 5'-TTCTGGGGAGTCCCATACTCTTG-3' T_m: 59.148
Expected PCR Product Length: 280
Expected CelI Cleavage Product Sizes: 149, 131
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr1:20261420-20261466

Rank: 86	Homology Score: 2	RVD Match Score: 2.962	Left Half-Site RVD Score: 6.821	Right Half-Site RVD Score: 5.653
	Genomic Region: Intron	Closest Gene: Col23a1
Match Type: R23L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-GAATA TTCATTGTTTTCCTAGG CTGCTGGGACAAAGAACCAGAGT  TTGGGGGTGGGGATGAA GGGAG-3'
	3'-CTTAT AAGTAACAAAAGGATCC GACGACCCTGTTTCTTGGTCTCA  AACCCCCACCCCTACTT CCCTC-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 010-036934773
Forward Primer: 5'-GGAGCATCTCTGTGTAACACTGG-3' T_m: 59.148
Reverse Primer: 5'-ACGAGGAGCCCGATACCTAC-3' T_m: 59.508
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 137, 133
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr10:36934880-36934936

Rank: 87	Homology Score: 2	RVD Match Score: 2.962	Left Half-Site RVD Score: 6.355	Right Half-Site RVD Score: 6.021
	Genomic Region: Intron	Closest Gene: Patl1
Match Type: R24L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-TTCAT TTGAGCATTAATCTGGT TTTTCCAATTAAGAATATGGTAGT GTGGGGCTGGGGATGTA GCTCA-3'
	3'-AAGTA AACTCGTAATTAGACCA AAAAGGTTAATTCTTATACCATCA CACCCCGACCCCTACAT CGAGT-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 001-214771817
Forward Primer: 5'-GTCCAGCACAGTCTGCTTATGCTA-3' T_m: 59.038
Reverse Primer: 5'-CCTTGAAAAGAGTGCTCATTGCCC-3' T_m: 59.038
Expected PCR Product Length: 414
Expected CelI Cleavage Product Sizes: 204, 210
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr1:214771990-214772047

Rank: 88	Homology Score: 2	RVD Match Score: 2.963	Left Half-Site RVD Score: 6.355	Right Half-Site RVD Score: 6.025
	Genomic Region: Intergenic	Closest Gene: Exoc4
Match Type: R20L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-CACAC TTGAGAGTTATTCAGAT   AGAGCGGTGAATATATTGTC   ATGGAGTTGGTAAGAGA CAAAG-3'
	3'-GTGTG AACTCTCAATAAGTCTA   TCTCGCCACTTATATAACAG   TACCTCAACCATTCTCT GTTTC-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. Warning! The sequence of this region of the genome is poorly mapped. 
Primer Index: 004-060388145
Forward Primer: 5'-GAGTCTGGGGCTATTTGTCTGG-3' T_m: 59.258
Reverse Primer: 5'-GGACTGAACTGAACCTGTAAGCC-3' T_m: 59.148
Expected PCR Product Length: 299
Expected CelI Cleavage Product Sizes: 131, 168
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr4:60388247-60388300

Rank: 89	Homology Score: 5	RVD Match Score: 2.965	Left Half-Site RVD Score: 6.55	Right Half-Site RVD Score: 5.759
	Genomic Region: Intergenic	Closest Gene: Odz2
Match Type: L23R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-TCATT TTCCTGCACACATCAAG TCCCATTTTATAGTCCCCAAATA  CGCAGGACAACATTGAA CAAGA-3'
	3'-AGTAA AAGGACGTGTGTAGTTC AGGGTAAAATATCAGGGGTTTAT  GCGTCCTGTTGTAACTT GTTCT-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 010-022508820
Forward Primer: 5'-GTGGTGGTGAGGGAAGAAACTAC-3' T_m: 59.148
Reverse Primer: 5'-CGCGAAGCCCCTTAATGGTC-3' T_m: 59.508
Expected PCR Product Length: 426
Expected CelI Cleavage Product Sizes: 159, 267
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr10:22508949-22509005

Rank: 90	Homology Score: 5	RVD Match Score: 2.968	Left Half-Site RVD Score: 6.686	Right Half-Site RVD Score: 5.798
	Genomic Region: Intergenic	Closest Gene: Galntl5
Match Type: R16L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-ACAAG TCCATTATTGTTACGGT     GGGAAGCAAGGCAGGC     ATGGCGCTGGGGAAGGA GCTGA-3'
	3'-TGTTC AGGTAATAACAATGCCA     CCCTTCGTTCCGTCCG     TACCGCGACCCCTTCCT CGACT-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 004-005451548
Forward Primer: 5'-CAGTTCTGTGGGGAGGAGCA-3' T_m: 59.508
Reverse Primer: 5'-GTGTGGCCTTGTAAGAGGAAGTG-3' T_m: 59.148
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 134, 136
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr4:5451655-5451704

Rank: 91	Homology Score: 2	RVD Match Score: 2.968	Left Half-Site RVD Score: 6.25	Right Half-Site RVD Score: 6.151
	Genomic Region: Intron	Closest Gene: Tspan18
Match Type: R21L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-GGCTG TTTAATGATATTCTGTT  TTATCTGCCCACCAAGAAAAG   AGGCAGCTGGGTATGGA GCAGG-3'
	3'-CCGAC AAATTACTATAAGACAA  AATAGACGGGTGGTTCTTTTC   TCCGTCGACCCATACCT CGTCC-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 003-077712956
Forward Primer: 5'-CCCTGTGGTCAGCAGCTCTT-3' T_m: 59.508
Reverse Primer: 5'-ATGAAGACTGCCTCATCAAGGGCC-3' T_m: 60.908
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 130, 140
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr3:77713057-77713111

Rank: 92	Homology Score: 5	RVD Match Score: 2.968	Left Half-Site RVD Score: 6.428	Right Half-Site RVD Score: 6.007
	Genomic Region: Intergenic	Closest Gene: Fut7
Match Type: R16L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-AGGTT TTAAGTGTTATTGTTAT     TTTCTAGTTCTTATGT     ATGGAGCTGGGGACCGA ACCTA-3'
	3'-TCCAA AATTCACAATAACAATA     AAAGATCAAGAATACA     TACCTCGACCCCTGGCT TGGAT-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 003-003587477
Forward Primer: 5'-TACAGATGGTTGTGAGCCACCATG-3' T_m: 59.038
Reverse Primer: 5'-GCACATACCCACACAGACATGCA-3' T_m: 59.148
Expected PCR Product Length: 267
Expected CelI Cleavage Product Sizes: 133, 134
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr3:3587583-3587632

Rank: 93	Homology Score: 2	RVD Match Score: 2.97	Left Half-Site RVD Score: 5.527	Right Half-Site RVD Score: 7.068
	Genomic Region: Intergenic	Closest Gene: Fndc3a
Match Type: L21R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-CCATC TCCCAACCCATCCCCAT  TCCTACATGTCAGCTCCCAAT   ACCAAAAAAACATTTTA CTTAG-3'
	3'-GGTAG AGGGTTGGGTAGGGGTA  AGGATGTACAGTCGAGGGTTA   TGGTTTTTTTGTAAAAT GAATC-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 015-052640368
Forward Primer: 5'-AACAGCCCTTTGGCTGTGGCCT-3' T_m: 61.288
Reverse Primer: 5'-CCTAGACACCTGGGTTTGGG-3' T_m: 59.508
Expected PCR Product Length: 356
Expected CelI Cleavage Product Sizes: 143, 213
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr15:52640482-52640536

Rank: 94	Homology Score: 2	RVD Match Score: 2.971	Left Half-Site RVD Score: 6.362	Right Half-Site RVD Score: 6.078
	Genomic Region: Intron	Closest Gene: Tgfa
Match Type: R13L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-GTGCT TTAAATCTTGTTCAGGA      CTCCAGCCTGGGT       TATGAGTTGGGAAGGAA CCTGG-3'
	3'-CACGA AATTTAGAACAAGTCCT      GAGGTCGGACCCA       ATACTCAACCCTTCCTT GGACC-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 004-120383597
Forward Primer: 5'-GAGGCTGGTCTCAAACTCACAATG-3' T_m: 59.038
Reverse Primer: 5'-AGGAAGAGACACAGAGACAGGG-3' T_m: 59.258
Expected PCR Product Length: 287
Expected CelI Cleavage Product Sizes: 156, 131
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr4:120383728-120383774

Rank: 95	Homology Score: 5	RVD Match Score: 2.973	Left Half-Site RVD Score: 6.525	Right Half-Site RVD Score: 5.967
	Genomic Region: Intergenic	Closest Gene: Cd59
Match Type: R24L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-AATGT TCCAGTTTCATTCTGTT CCTGTGATAAATCACTATGACTAC AGGAACCTGGGGAGGAA AGGGT-3'
	3'-TTACA AGGTCAAAGTAAGACAA GGACACTATTTAGTGATACTGATG TCCTTGGACCCCTCCTT TCCCA-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 003-089208603
Forward Primer: 5'-CCGATGCCTGGTGTATTTGTCAGT-3' T_m: 59.038
Reverse Primer: 5'-GCAAGCCAACAATTGGCATTCTCC-3' T_m: 59.038
Expected PCR Product Length: 299
Expected CelI Cleavage Product Sizes: 163, 136
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr3:89208735-89208792

Rank: 96	Homology Score: 10	RVD Match Score: 2.975	Left Half-Site RVD Score: 7.012	Right Half-Site RVD Score: 5.338
	Genomic Region: Intergenic	Closest Gene: Cntnap5b
Match Type: L22R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-CTCTT TCTCTTCCTAGCTCCCT  GCAATTGTATCCTGCTGTGAGT  ACCAGATCAAAAGTGAA TATTT-3'
	3'-GAGAA AGAGAAGGATCGAGGGA  CGTTAACATAGGACGACACTCA  TGGTCTAGTTTTCACTT ATAAA-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 013-002441765
Forward Primer: 5'-CTGTCTCTGTCTCTCTTAGTTCCC-3' T_m: 59.038
Reverse Primer: 5'-CCCTTTTTGCCTTTCCATGCAGTG-3' T_m: 59.038
Expected PCR Product Length: 336
Expected CelI Cleavage Product Sizes: 177, 159
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr13:2441912-2441967

Rank: 97	Homology Score: 13	RVD Match Score: 2.975	Left Half-Site RVD Score: 6.205	Right Half-Site RVD Score: 6.241
	Genomic Region: Promoter	Closest Gene: Cln5
Match Type: R14L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-AGTGT TTTATTGTTGTTATGGT      TTGGCTGGTGGTGG      CTGCAGGTGGGCAGAAA GCACC-3'
	3'-TCACA AAATAACAACAATACCA      AACCGACCACCACC      GACGTCCACCCGTCTTT CGTGG-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 015-087079625
Forward Primer: 5'-CAATCACTCCCTGCAATCCCC-3' T_m: 59.378
Reverse Primer: 5'-TGACGCCTCCACTGTCCTAG-3' T_m: 59.508
Expected PCR Product Length: 326
Expected CelI Cleavage Product Sizes: 142, 184
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr15:87079741-87079788

Rank: 98	Homology Score: 8	RVD Match Score: 2.977	Left Half-Site RVD Score: 5.814	Right Half-Site RVD Score: 6.755
	Genomic Region: Intron	Closest Gene: Map2k1
Match Type: L15R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-CAGCC TTCCTTCCCATCTACAG     AAGAAATCAGCGAAG      AGCAGAACTCCCCTGAA GTCAG-3'
	3'-GTCGG AAGGAAGGGTAGATGTC     TTCTTTAGTCGCTTC      TCGTCTTGAGGGGACTT CAGTC-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 008-068387105
Forward Primer: 5'-CCGACTAGCTTCTCCCATGAC-3' T_m: 59.378
Reverse Primer: 5'-TTACTGTCGGTGTCACAGTGCC-3' T_m: 59.258
Expected PCR Product Length: 322
Expected CelI Cleavage Product Sizes: 191, 131
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr8:68387270-68387318

Rank: 99	Homology Score: 2	RVD Match Score: 2.978	Left Half-Site RVD Score: 6.301	Right Half-Site RVD Score: 6.164
	Genomic Region: Intergenic	Closest Gene: Asah1
Match Type: L23R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-AACTC TCCACTCCCATCTCCAT TCCACCCCAGCTGTGTATGACTG  AATAGAATAGCACTGCA AAGGC-3'
	3'-TTGAG AGGTGAGGGTAGAGGTA AGGTGGGGTCGACACATACTGAC  TTATCTTATCGTGACGT TTCCG-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 016-053786431
Forward Primer: 5'-CTGCAAGTTTGAGTACTGGACAGC-3' T_m: 59.038
Reverse Primer: 5'-CCAGTCTCCCATGCTGTTTACTTC-3' T_m: 59.038
Expected PCR Product Length: 345
Expected CelI Cleavage Product Sizes: 133, 212
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr16:53786534-53786590

Rank: 100	Homology Score: 10	RVD Match Score: 2.979	Left Half-Site RVD Score: 5.706	Right Half-Site RVD Score: 6.705
	Genomic Region: Intergenic	Closest Gene: Plcl2
Match Type: R21L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-ATGTT TTCAGTGTTGCTCCTGT  TGGGTAAAGGAGGAATGTTCC   CTGGATCGGGGCTGGTA TAGTG-3'
	3'-TACAA AAGTCACAACGAGGACA  ACCCATTTCCTCCTTACAAGG   GACCTAGCCCCGACCAT ATCAC-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 009-004673112
Forward Primer: 5'-AATCCACCGAGCCTAGTAACTGC-3' T_m: 59.148
Reverse Primer: 5'-TACACTGCGCTCACACAGCAAG-3' T_m: 59.258
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 135, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr9:4673218-4673272

Plate format Oligo Order Form
Well Name Sequence 5' mods Internal Notes
Primer Plate #1
A1 006-138443970F GGAATCGGCACATGCAGATCTTTG Tesson_IgM_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
A2 006-138443970R TCTGGTCCTCAGAGAGTCAGTC Tesson_IgM_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
B1 001-252428669F GTTGTGTTTCAGGACAGCACTACC Tesson_IgM_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
B2 001-252428669R GTTGGAGAGGCCACATTCTTAACC Tesson_IgM_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
C1 00X-027254372F CCATTATCGCTAGCCTAGCTTACG Tesson_IgM_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
C2 00X-027254372R GACTGGGAATAAGTGGTGTGGTAC Tesson_IgM_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
D1 009-007190270F AGTTGGGCTGGTAGAAATTGCTGG Tesson_IgM_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
D2 009-007190270R CTGAGTGAAACAAACGGCTCCGA Tesson_IgM_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
E1 006-090557385F GTCCCCACAGGCATATGATCG Tesson_IgM_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
E2 006-090557385R GTGCTTCGGGCAGTAGAGCT Tesson_IgM_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
F1 020-043706993F GCAGAAATGGATAGTTTGTGGGGG Tesson_IgM_Ranking-RVDs_5T	Primer pair generated using optimal parameters. Warning! The sequence of this region of the genome is poorly mapped. 
F2 020-043706993R CCGCCAAGCCCTTTTTAAGTACTG Tesson_IgM_Ranking-RVDs_5T	Primer pair generated using optimal parameters. Warning! The sequence of this region of the genome is poorly mapped. 
G1 004-126653383F CCATGTGACCTTTGGACTACATGC Tesson_IgM_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
G2 004-126653383R GAGAGCCACTGACTACCATAACAC Tesson_IgM_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
H1 014-082098705F AGCCAAGGAAGAAACCTGTGCAGC Tesson_IgM_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
H2 014-082098705R CTGTGGATGGCTAGAGTGTACTCA Tesson_IgM_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
A3 019-057896612F CCCAGTGCTGGGGTTACAAACA Tesson_IgM_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
A4 019-057896612R TCTTAGCCAGGAATGAGGCTGC Tesson_IgM_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
B3 005-123636672F CTCTGAGTGACACGAATTAGTGGG Tesson_IgM_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
B4 005-123636672R GGAGTGTGTCACTAAGGCTGG Tesson_IgM_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
C3 003-128243778F CAACCACACTGCAGACCAGG Tesson_IgM_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
C4 003-128243778R GCCATGGATCTGATATTGGTGGCT Tesson_IgM_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
D3 003-100952790F GCCATGAAGAAGACATGCAGGAG Tesson_IgM_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
D4 003-100952790R GGATCACTTGAAATGTGGAGCTTCCTAC Tesson_IgM_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
E3 020-016460972F GTCAAGGACGAAGTCATCTGGAG Tesson_IgM_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
E4 020-016460972R GACAAGCTGACTGCTGCTAGAG Tesson_IgM_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
F3 002-204001007F AATTTATGGGCTCTGTGCCGTGC Tesson_IgM_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
F4 002-204001007R CCCAGTGCTAAGTTGAGTGTGGC Tesson_IgM_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
G3 003-157836620F GGCAAAGCTGCCTGTCACCA Tesson_IgM_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
G4 003-157836620R GCTTCAGGCACAGCTGGATC Tesson_IgM_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
H3 002-159432579F GCTAAATCCCCAACCCTGCAG Tesson_IgM_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
H4 002-159432579R GTGTTTACTTGCTCAAACACCCGG Tesson_IgM_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
A5 001-127719260F CCAACATTCTCCCAAGAGATTCTGGAAAC Tesson_IgM_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
A6 001-127719260R GTTCCAGGTCAACCAGGACTAC Tesson_IgM_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
B5 006-075907048F GGTGTCCTCATTCACCTCTGACAA Tesson_IgM_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
B6 006-075907048R GTGTTTGGAACATCCTCTGCGAC Tesson_IgM_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
C5 001-140972716F GGGACCACCTCAGCTAAGGA Tesson_IgM_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
C6 001-140972716R GTGGGGTTGTTGGTTTTCAGCTC Tesson_IgM_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
D5 00X-016509931F CTACCAATGTCTATGGAGTGACCG Tesson_IgM_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
D6 00X-016509931R GCCTAGAGTATCCCAAATGTGCAC Tesson_IgM_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
E5 004-135905706F GACAACGACTGCGTTCTATTTGGG Tesson_IgM_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
E6 004-135905706R TCACGAGCCCTGGTTATTGTTTGG Tesson_IgM_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
F5 007-067524783F TGATCAGAGCCTGCCCCTTC Tesson_IgM_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
F6 007-067524783R GACAGGGTGCTTCCTCACAATAC Tesson_IgM_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
G5 010-013862379F CATAGGTGGGGCCTTATCAGAG Tesson_IgM_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
G6 010-013862379R GGTCCCTAGTCAAGGACATTGTCT Tesson_IgM_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
H5 003-124119953F CAGTAGCTGCCTTTGACCAGGA Tesson_IgM_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
H6 003-124119953R CAAACCAGACAATGGCTTCTGCTC Tesson_IgM_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
A7 001-001765937F TTCTAGCCAAGAGGTCCAAAGGCC Tesson_IgM_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
A8 001-001765937R TGCATGGCCTCTGCATCAGC Tesson_IgM_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
B7 005-082146486F GCCAGAGGCCCTGGTCTTAT Tesson_IgM_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
B8 005-082146486R CTCCATTCCCTACTACAGAGAGTG Tesson_IgM_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
C7 001-026132578F CATTCAGATACTCTGAAGCGAAACATGTTAAGG Tesson_IgM_Ranking-RVDs_5T	Warning! Primer pair was generated using suboptimal parameters. 
C8 001-026132578R CCCCTGACCAATACTTTGCAAACG Tesson_IgM_Ranking-RVDs_5T	Warning! Primer pair was generated using suboptimal parameters. 
D7 009-019047855F GTGGCTTTGGTCCTCATCTGTC Tesson_IgM_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
D8 009-019047855R CCTCTTTCGGAGATAGGCATGTTC Tesson_IgM_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
E7 018-068645313F CTGGGAGCCTGGAAATTTGTCTG Tesson_IgM_Ranking-RVDs_5T	Warning! Primer pair was generated using suboptimal parameters. 
E8 018-068645313R GGACACAAGCATATATTATTCTTTGGATGTGCTC Tesson_IgM_Ranking-RVDs_5T	Warning! Primer pair was generated using suboptimal parameters. 
F7 004-003843423F GGTGACCAATACCAACCATCAGG Tesson_IgM_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
F8 004-003843423R CCTCCTTTCAGGAAGGCTCTTTCT Tesson_IgM_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
G7 012-031851371F GGGTGCCTGACTGCTATATTTCC Tesson_IgM_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
G8 012-031851371R TTGCCTGCCACAACCATGCATC Tesson_IgM_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
H7 018-072979833F CCGAGTGAAGGTAGCAGAACTCAG Tesson_IgM_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
H8 018-072979833R TTGCTTAGAGGGACCCAGGAAGA Tesson_IgM_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
A9 017-030652372F TCCGCTCCTGCACCAGGTTT Tesson_IgM_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
A10 017-030652372R GGAGTAAGTCAGCATTGAGTGGG Tesson_IgM_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
B9 002-139129605F CCCATGTGCAGAAGGAGTTGTAC Tesson_IgM_Ranking-RVDs_5T	Primer pair generated using optimal parameters. Warning! The sequence of this region of the genome is poorly mapped. 
B10 002-139129605R GTTCCAGGTCAGTCAGGGATATAC Tesson_IgM_Ranking-RVDs_5T	Primer pair generated using optimal parameters. Warning! The sequence of this region of the genome is poorly mapped. 
C9 002-040496981F GTGTGTGTGTGTGTGTGTGTGG Tesson_IgM_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
C10 002-040496981R TCTTCCACACCCTGTGCACC Tesson_IgM_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
D9 006-052366880F GGGGGAGGGAACCTTAATAGGA Tesson_IgM_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
D10 006-052366880R CAGAAAGTGCTGAGCCAAGTGAC Tesson_IgM_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
E9 004-174994043F GTGCGGCTCTGTCCTCACAT Tesson_IgM_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
E10 004-174994043R GATTAAATTGCATGCAGCTGCACTAACTCC Tesson_IgM_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
F9 003-060396362F CCACTGGGAGTGTATTCGGTATG Tesson_IgM_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
F10 003-060396362R GTAAACAGGCCTCTACAGAACGG Tesson_IgM_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
G9 008-005163674F AGGAGGAGGAGGAGGAGGAA Tesson_IgM_Ranking-RVDs_5T	Primer pair generated using optimal parameters. Warning! The sequence of this region of the genome is poorly mapped. 
G10 008-005163674R TATATGTGGCTGGAGTCACAGGTC Tesson_IgM_Ranking-RVDs_5T	Primer pair generated using optimal parameters. Warning! The sequence of this region of the genome is poorly mapped. 
H9 008-120556664F CAATTGAATCAGGACCCGGTTTGG Tesson_IgM_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
H10 008-120556664R GGTCAGTAATGATGGGTCTCTCC Tesson_IgM_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
A11 004-006079924F GTGTTCTACCCTCAGCCCAACAA Tesson_IgM_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
A12 004-006079924R GGGAAGGGCACACTGTTTATTACC Tesson_IgM_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
B11 004-068260584F CCCGTCTCTGTCAAACATGCTAAG Tesson_IgM_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
B12 004-068260584R GGAACTTCATAACGACTGCGGTTG Tesson_IgM_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
C11 004-068285787F GAGTGTGCTGAGTCACTCATCTCT Tesson_IgM_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
C12 004-068285787R GGAACTTCATAACGACTGCGGTTG Tesson_IgM_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
D11 005-161756246F CTCTAGGGGCTAAGTATCTCAGCT Tesson_IgM_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
D12 005-161756246R GGTGGCAAAATCTAAACCCTGTGG Tesson_IgM_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
E11 006-076453434F GGACCATTGTCAAATCAGGTGGG Tesson_IgM_Ranking-RVDs_5T	Primer pair generated using optimal parameters. Warning! The sequence of this region of the genome is poorly mapped. 
E12 006-076453434R GCCAGGGTGCCATGATTCCTA Tesson_IgM_Ranking-RVDs_5T	Primer pair generated using optimal parameters. Warning! The sequence of this region of the genome is poorly mapped. 
F11 009-052013154F GAGGCTGAGCTTGGCTTGCTT Tesson_IgM_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
F12 009-052013154R GCAAGGTCACATAGTGTCACTTGG Tesson_IgM_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
G11 015-073002183F CCTTGCTCTCCATCCCACAC Tesson_IgM_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
G12 015-073002183R GAGTCTTGGGTCTGATTGCTTGG Tesson_IgM_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
H11 005-170773963F CCAAGGTTGCTGCCAGTTCAGCT Tesson_IgM_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
H12 005-170773963R AGGAGCTGGAGAGCCAAGTG Tesson_IgM_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
Primer Plate #2
A1 00X-131557987F TTTTGGATGTCAGCTATAGGGTTGGAGAAAATCTT Tesson_IgM_Ranking-RVDs_5T	Warning! Primer pair was generated using suboptimal parameters. 
A2 00X-131557987R CAACAAATGATGCTAATCTAACTGGATGTCTACATG Tesson_IgM_Ranking-RVDs_5T	Warning! Primer pair was generated using suboptimal parameters. 
B1 00X-040773744F GGGTGAAACAGCATGTGGCATC Tesson_IgM_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
B2 00X-040773744R GGTCCTATTAACTCCTACCTCCAG Tesson_IgM_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
C1 017-087721857F CCAAGGATTTCCTGACATCGTGTG Tesson_IgM_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
C2 017-087721857R TGTGAGAAACCTGGGTGAGCC Tesson_IgM_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
D1 00X-146103263F CTACACTCCTTTTTGTTGGGTGGG Tesson_IgM_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
D2 00X-146103263R CTCTGCTGCAGAGCCTTTTTTCC Tesson_IgM_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
E1 014-081690069F GTGTCACTGAGGGTGTGCTTTG Tesson_IgM_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
E2 014-081690069R GCTAGCTGTGTGGCATTAAGGAAG Tesson_IgM_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
F1 007-029017126F CTTAGCTGGGGTATTACTGCTGTG Tesson_IgM_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
F2 007-029017126R AAACGCTTCACTGGGGGTGG Tesson_IgM_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
G1 006-079162173F ACACAGAAGGCTCTCCAGAGAC Tesson_IgM_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
G2 006-079162173R ACTTTGATCTCACTTCAGGTCCCC Tesson_IgM_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
H1 017-072075714F GCCTGTCACAGTTTGTCTCTTGC Tesson_IgM_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
H2 017-072075714R GCAGGCTTTTGCATGTTGTCTGAG Tesson_IgM_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
A3 010-062612960F GAGCTGGCACTTCATGAAGGTC Tesson_IgM_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
A4 010-062612960R GCCCGTGGACTACAGAGACT Tesson_IgM_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
B3 009-045551124F AAGCAGCACCCCTCCAAGCA Tesson_IgM_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
B4 009-045551124R GTGGGCAAAGCAGAAGTAAGCTC Tesson_IgM_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
C3 013-033948884F GAGGCAGCCATCCACATTGTATC Tesson_IgM_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
C4 013-033948884R CCTTCAGTCGGTGTGGTAGAAAAC Tesson_IgM_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
D3 011-031478392F GATCTGATCCCCTTTTCTGGTGTG Tesson_IgM_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
D4 011-031478392R CTTCTTTAGCACGTCGCCCC Tesson_IgM_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
E3 005-008485774F CACACACACACACACACACACAC Tesson_IgM_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
E4 005-008485774R CTGCCTCCTAATATGGCCACG Tesson_IgM_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
F3 002-178282654F GAGCTACATTTCTGCCCCGAAG Tesson_IgM_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
F4 002-178282654R CCACGCAGACAAATAAGCACACG Tesson_IgM_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
G3 010-030648298F CCCACCAGTCCAAATGGTGGTA Tesson_IgM_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
G4 010-030648298R TGCAAAGCTGCTTGCTCTGGC Tesson_IgM_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
H3 001-187665749F GGAGGGAAGGAGGGAAGGAA Tesson_IgM_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
H4 001-187665749R ACACCAGTCTTGCTGAGGGC Tesson_IgM_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
A5 001-242536006F TGGAGGTTGGGAGAGTAGAGG Tesson_IgM_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
A6 001-242536006R TGAAGGAGGGAACACTCAGCAG Tesson_IgM_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
B5 010-041837672F CCCACACTCCTGCTTCTGTATC Tesson_IgM_Ranking-RVDs_5T	Primer pair generated using optimal parameters. Warning! The sequence of this region of the genome is poorly mapped. 
B6 010-041837672R GTCCATGCTGTCACCAGACATC Tesson_IgM_Ranking-RVDs_5T	Primer pair generated using optimal parameters. Warning! The sequence of this region of the genome is poorly mapped. 
C5 008-023607288F CGGAAGCCATGTTCAGGTCTTCA Tesson_IgM_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
C6 008-023607288R CAGACCAGAAGACTGTGTGATTGC Tesson_IgM_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
D5 002-055689862F CTGAGTTTGAGGCCAGTATGGG Tesson_IgM_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
D6 002-055689862R GCTGCCATTCCACTGAAGGTTG Tesson_IgM_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
E5 004-106165776F ATGCTTAGGCTACTGTGTCTGGC Tesson_IgM_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
E6 004-106165776R TAGCAGGGTCATGTGGTACAGC Tesson_IgM_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
F5 00X-010426535F GTTCTCTGCATCCATGCCATGTG Tesson_IgM_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
F6 00X-010426535R CCTCAGCATCAGTTACTGCTTCC Tesson_IgM_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
G5 001-171517384F CACCTTGTGTTTCCAGAACTCTGC Tesson_IgM_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
G6 001-171517384R CTAGTCCTATCACATCCACGGTC Tesson_IgM_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
H5 014-071610248F GCTCCAGAAAGTGCCTTGTTCTTG Tesson_IgM_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
H6 014-071610248R GCCTTTGCCCGCATGGTTTC Tesson_IgM_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
A7 003-048025715F ACTGTCACACTGCTGTCACGG Tesson_IgM_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
A8 003-048025715R GACTGTCTTCGCAATAGGGTTATCTCATC Tesson_IgM_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
B7 011-029469068F CTATTTCCGTGTCTTTGGGGATGC Tesson_IgM_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
B8 011-029469068R GCTTTCCACGTCTATGTCCTGGT Tesson_IgM_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
C7 011-030381700F GCTTTCCACGTCTATGTCCTGGT Tesson_IgM_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
C8 011-030381700R CTATTTCCGTGTCTTTGGGGATGC Tesson_IgM_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
D7 011-030396776F CTATTTCCGTGTCTTTGGGGATGC Tesson_IgM_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
D8 011-030396776R GCTTTCCACGTCTATGTCCTGGT Tesson_IgM_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
E7 002-106965669F ATAGTGGCCAGTGCCTGGAC Tesson_IgM_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
E8 002-106965669R CCAGGAGAAAAGGCCATCTTCCA Tesson_IgM_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
F7 001-138061468F CCGTGAAGCAGGTCACAAAGC Tesson_IgM_Ranking-RVDs_5T	Primer pair generated using optimal parameters. Warning! The sequence of this region of the genome is poorly mapped. 
F8 001-138061468R CAGCAAGGGAAGTGCTACTTGATG Tesson_IgM_Ranking-RVDs_5T	Primer pair generated using optimal parameters. Warning! The sequence of this region of the genome is poorly mapped. 
G7 014-085768096F GGGAATGGTTAGCCCCATCTC Tesson_IgM_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
G8 014-085768096R CATGAGGAAACAGTTCAGGAGGG Tesson_IgM_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
H7 003-007890866F CAGTCTGTTGTTAAGGGATCGAGC Tesson_IgM_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
H8 003-007890866R GACACTGAGACTTGCATAGGCC Tesson_IgM_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
A9 014-103592966F TACAGGAGGAGTGGCCTTGTG Tesson_IgM_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
A10 014-103592966R GCGTCAAGGTAAAATGACCAAGGG Tesson_IgM_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
B9 005-113632907F GGAGGAATCTCTAGAAAGTCCCAG Tesson_IgM_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
B10 005-113632907R GGCTCCTATCTGCAGGTCCA Tesson_IgM_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
C9 009-042648255F CCAGCCCCAACTAAATATTTGCCC Tesson_IgM_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
C10 009-042648255R GAGGAGACTGGGAGTAAGCTTTG Tesson_IgM_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
D9 008-035238872F CTGAGGCCTGAGCTTTCTCG Tesson_IgM_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
D10 008-035238872R GTGTGCCACTATGCCAGACC Tesson_IgM_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
E9 001-020261296F AAGGGATGCCTCAGCATGGG Tesson_IgM_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
E10 001-020261296R TTCTGGGGAGTCCCATACTCTTG Tesson_IgM_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
F9 010-036934773F GGAGCATCTCTGTGTAACACTGG Tesson_IgM_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
F10 010-036934773R ACGAGGAGCCCGATACCTAC Tesson_IgM_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
G9 001-214771817F GTCCAGCACAGTCTGCTTATGCTA Tesson_IgM_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
G10 001-214771817R CCTTGAAAAGAGTGCTCATTGCCC Tesson_IgM_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
H9 004-060388145F GAGTCTGGGGCTATTTGTCTGG Tesson_IgM_Ranking-RVDs_5T	Primer pair generated using optimal parameters. Warning! The sequence of this region of the genome is poorly mapped. 
H10 004-060388145R GGACTGAACTGAACCTGTAAGCC Tesson_IgM_Ranking-RVDs_5T	Primer pair generated using optimal parameters. Warning! The sequence of this region of the genome is poorly mapped. 
A11 010-022508820F GTGGTGGTGAGGGAAGAAACTAC Tesson_IgM_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
A12 010-022508820R CGCGAAGCCCCTTAATGGTC Tesson_IgM_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
B11 004-005451548F CAGTTCTGTGGGGAGGAGCA Tesson_IgM_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
B12 004-005451548R GTGTGGCCTTGTAAGAGGAAGTG Tesson_IgM_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
C11 003-077712956F CCCTGTGGTCAGCAGCTCTT Tesson_IgM_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
C12 003-077712956R ATGAAGACTGCCTCATCAAGGGCC Tesson_IgM_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
D11 003-003587477F TACAGATGGTTGTGAGCCACCATG Tesson_IgM_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
D12 003-003587477R GCACATACCCACACAGACATGCA Tesson_IgM_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
E11 015-052640368F AACAGCCCTTTGGCTGTGGCCT Tesson_IgM_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
E12 015-052640368R CCTAGACACCTGGGTTTGGG Tesson_IgM_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
F11 004-120383597F GAGGCTGGTCTCAAACTCACAATG Tesson_IgM_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
F12 004-120383597R AGGAAGAGACACAGAGACAGGG Tesson_IgM_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
G11 003-089208603F CCGATGCCTGGTGTATTTGTCAGT Tesson_IgM_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
G12 003-089208603R GCAAGCCAACAATTGGCATTCTCC Tesson_IgM_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
H11 013-002441765F CTGTCTCTGTCTCTCTTAGTTCCC Tesson_IgM_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
H12 013-002441765R CCCTTTTTGCCTTTCCATGCAGTG Tesson_IgM_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
Primer Plate #3
A1 015-087079625F CAATCACTCCCTGCAATCCCC Tesson_IgM_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
A2 015-087079625R TGACGCCTCCACTGTCCTAG Tesson_IgM_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
B1 008-068387105F CCGACTAGCTTCTCCCATGAC Tesson_IgM_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
B2 008-068387105R TTACTGTCGGTGTCACAGTGCC Tesson_IgM_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
C1 016-053786431F CTGCAAGTTTGAGTACTGGACAGC Tesson_IgM_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
C2 016-053786431R CCAGTCTCCCATGCTGTTTACTTC Tesson_IgM_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
D1 009-004673112F AATCCACCGAGCCTAGTAACTGC Tesson_IgM_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
D2 009-004673112R TACACTGCGCTCACACAGCAAG Tesson_IgM_Ranking-RVDs_5T	Primer pair generated using optimal parameters.
1579 total sites were located
Mismatch Types	Genomic Regions
0 and 0: 1 3 and 3: 1 2 and 5: 2 3 and 4: 6 3 and 5: 49 4 and 4: 19 4 and 5: 322 5 and 5: 1179	Exons: 23 Promoters: 8 Introns: 404 Intergenic: 1144