PROGNOS Summary for Tesson_IgM_Ranking-RVDs

The rn4 genome was searched for the TALEN pair targeting:
5'- TTCCTGCCCAGCTCCAT NN..NN ACCAGAACAACACTGAA -3'

Using these RVDs:
Left:  01NG02HD03HD04NG05NN06HD07HD08HD09NI10NN11HD12NG13HD14HD15NI16NG
Right: 01NG02HD03NI04NN05NG06NN07NG08NG09NN10NG11NG12HD13NG14NN15NN16NG

Up to 5 mismatches were allowed in each nuclease half-site.

Allowed spacing distances were: 12 13 14 15 16 17 18 19 20 21 22 23 24

Only heterodimers of the nuclease pair were included in the output

2541 total sites were located
Mismatch Types
Genomic Regions
  0 and 0: 1
  3 and 3: 1
  2 and 5: 3
  3 and 4: 12
  3 and 5: 61
  4 and 4: 23
  4 and 5: 538
  5 and 5: 1902
  Exons: 47
  Promoters: 13
  Introns: 646
  Intergenic: 1835


The top 100 sites are ranked below according to a weighting of the RVD base preferences.
For the full output, click here.

This output will be deleted from the server one week after it is generated.  Please save your results.

Primer pairs were designed using the 400 bp of sequence on either side of the cleavage site to melt close to 60 °C

For easy visualization of cleavage on gels:
The smallest fragment will be longer than 130 bp, which is also the minimum separation distance between uncleaved and cleaved products.
The two cleavage products will be separated by a minimum size of 0 bp
To optimize assay conditions, the amplicons have a minimum length of 260 bp, a maximum length of 450 bp, and an optimal length of 270 bp.
Rank: 1	Homology Score: 72	RVD Match Score: 2	Left Half-Site RVD Score: 3.404	Right Half-Site RVD Score: 2.361
	Genomic Region: Intergenic	Closest Gene: Adam6	Caution! This site scores very closely to the intended nuclease target. The potential for cleavage at this site is of great concern.
Match Type: R17L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-ATGAC TTCAGTGTTGTTCTGGT    AGTTCCAGGAGAAGGAA     ATGGAGCTGGGCAGGAA GTCCC-3'
	3'-TACTG AAGTCACAACAAGACCA    TCAAGGTCCTCTTCCTT     TACCTCGACCCGTCCTT CAGGG-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 006-138443970
Forward Primer: 5'-GGAATCGGCACATGCAGATCTTTG-3' Tm: 59.038
Reverse Primer: 5'-TCTGGTCCTCAGAGAGTCAGTC-3' Tm: 59.258
Expected PCR Product Length: 325
Expected CelI Cleavage Product Sizes: 194, 131
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr6:138444137-138444187

Rank: 2	Homology Score: 10	RVD Match Score: 2.614	Left Half-Site RVD Score: 5.634	Right Half-Site RVD Score: 3.899
	Genomic Region: Intron	Closest Gene: Sorcs1
Match Type: L23R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-TGATG CTGCTGCTCAACTCCCT TGCTAACAACTGAATACATCTGT  ATCAGAACAACAGTGAG CAAAA-3'
	3'-ACTAC GACGACGAGTTGAGGGA ACGATTGTTGACTTATGTAGACA  TAGTCTTGTTGTCACTC GTTTT-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 001-256112389
Forward Primer: 5'-CCTGGATAAGTCAGTAAAGGGTGG-3' Tm: 59.038
Reverse Primer: 5'-CCTTGGTGGGACCTGATACAGT-3' Tm: 59.258
Expected PCR Product Length: 353
Expected CelI Cleavage Product Sizes: 204, 149
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr1:256112563-256112619

Rank: 3	Homology Score: 5	RVD Match Score: 2.615	Left Half-Site RVD Score: 5.463	Right Half-Site RVD Score: 4.296
	Genomic Region: Intergenic	Closest Gene: Chsy1
Match Type: R18L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-AAGGG ATCAGCCTCATTCTGGT    AGCTGGTGTGTGGTGGCA    ATGGAGGTGGGATGGAG ATGTT-3'
	3'-TTCCC TAGTCGGAGTAAGACCA    TCGACCACACACCACCGT    TACCTCCACCCTACCTC TACAA-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 001-120607423
Forward Primer: 5'-AGGTCAGTGTCCCTGGCCTT-3' Tm: 59.508
Reverse Primer: 5'-GGCTGGCCAATTCACAATCCTC-3' Tm: 59.258
Expected PCR Product Length: 382
Expected CelI Cleavage Product Sizes: 133, 249
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr1:120607528-120607579

Rank: 4	Homology Score: 5	RVD Match Score: 2.622	Left Half-Site RVD Score: 4.296	Right Half-Site RVD Score: 5.521
	Genomic Region: Intergenic	Closest Gene: Snhg11
Match Type: L13R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-TCCGG CTCCTCCCCATCTCCAC      ACACCTGAGAATA       ACTAGAATAACAAAGAG CGGCA-3'
	3'-AGGCC GAGGAGGGGTAGAGGTG      TGTGGACTCTTAT       TGATCTTATTGTTTCTC GCCGT-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 003-149194978
Forward Primer: 5'-CTTGTTAGGTGAAGCAGGATGGC-3' Tm: 59.148
Reverse Primer: 5'-TCACACCGGTGCTGAAGGAAC-3' Tm: 59.378
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 136, 134
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr3:149195089-149195135

Rank: 5	Homology Score: 13	RVD Match Score: 2.639	Left Half-Site RVD Score: 4.477	Right Half-Site RVD Score: 5.426
	Genomic Region: Intergenic	Closest Gene: Tssc1
Match Type: L17R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-GGGGG CTCCTGCCCAGATCCTT    CTCTGCCAGGCTGCCCC     TCCAGCACAATATTGAA TCTCA-3'
	3'-CCCCC GAGGACGGGTCTAGGAA    GAGACGGTCCGACGGGG     AGGTCGTGTTATAACTT AGAGT-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 006-046986724
Forward Primer: 5'-CTTCTGGCTTAGTTTCCGTCTGTC-3' Tm: 59.038
Reverse Primer: 5'-CTCAGCTTGCCCAAGGGTTATAG-3' Tm: 59.148
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 130, 140
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr6:46986827-46986877

Rank: 6	Homology Score: 5	RVD Match Score: 2.647	Left Half-Site RVD Score: 6.208	Right Half-Site RVD Score: 3.971
	Genomic Region: Intergenic	Closest Gene: Btc
Match Type: R14L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-GCTCT TTCAACACTGTTCTGGG      TGATGGGTTGCATC      ATGGAGGTGGGGAGGTC TGCTC-3'
	3'-CGAGA AAGTTGTGACAAGACCC      ACTACCCAACGTAG      TACCTCCACCCCTCCAG ACGAG-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 014-018268832
Forward Primer: 5'-TTTCATGGCCTTCTGCAGCCATGT-3' Tm: 59.038
Reverse Primer: 5'-GCAGCAAACACACTACAAATGGGG-3' Tm: 59.038
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 132, 138
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr14:18268938-18268985

Rank: 7	Homology Score: 18	RVD Match Score: 2.668	Left Half-Site RVD Score: 5.677	Right Half-Site RVD Score: 4.477
	Genomic Region: Intron	Closest Gene: Pdcd11
Match Type: R16L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-TTGGA TGCAGACTTGTTCTGGT     CCTGTTAGCAGACCTC     AGGGATCTGGGGAGGAA TTAGC-3'
	3'-AACCT ACGTCTGAACAAGACCA     GGACAATCGTCTGGAG     TCCCTAGACCCCTCCTT AATCG-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 001-252428669
Forward Primer: 5'-GTTGTGTTTCAGGACAGCACTACC-3' Tm: 59.038
Reverse Primer: 5'-GTTGGAGAGGCCACATTCTTAACC-3' Tm: 59.038
Expected PCR Product Length: 308
Expected CelI Cleavage Product Sizes: 162, 146
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr1:252428804-252428853

Rank: 8	Homology Score: 13	RVD Match Score: 2.67	Left Half-Site RVD Score: 6.001	Right Half-Site RVD Score: 4.258
	Genomic Region: Intron	Closest Gene: Acvrl1
Match Type: R21L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-AAGGC TTCATTGAGGTTCTGGT  CTTCATGATCACAGAAGCGGC   CTGGAGTTGGGAAGGAG GAAGA-3'
	3'-TTCCG AAGTAACTCCAAGACCA  GAAGTACTAGTGTCTTCGCCG   GACCTCAACCCTTCCTC CTTCT-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 007-139879252
Forward Primer: 5'-AAAGGCTCATTAGACACGCTCAGG-3' Tm: 59.038
Reverse Primer: 5'-CACGTCTCTGCAGACCCCAT-3' Tm: 59.508
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 134, 136
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr7:139879357-139879411

Rank: 9	Homology Score: 5	RVD Match Score: 2.683	Left Half-Site RVD Score: 5.043	Right Half-Site RVD Score: 5.086
	Genomic Region: Intron	Closest Gene: Top1
Match Type: L21R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-TGGAC TTCCTGCACCACTCAAT  GGCTGTGATTATCAGCACAAG   ACATGAACAATATTGAG CCTGT-3'
	3'-ACCTG AAGGACGTGGTGAGTTA  CCGACACTAATAGTCGTGTTC   TGTACTTGTTATAACTC GGACA-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 003-151465311
Forward Primer: 5'-CACACACACACACACACACACAC-3' Tm: 59.148
Reverse Primer: 5'-CACCAGTGACAACAGCACTTGAG-3' Tm: 59.148
Expected PCR Product Length: 330
Expected CelI Cleavage Product Sizes: 186, 144
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr3:151465468-151465522

Rank: 10	Homology Score: 13	RVD Match Score: 2.696	Left Half-Site RVD Score: 3.606	Right Half-Site RVD Score: 7.262
	Genomic Region: Intergenic	Closest Gene: Robo1
Match Type: L19R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-CTGTA GTCCTACCCAGCTTCAT   TGTCTATGACACAACGATA    ACCAGCACAGCACAGTA ACCTG-3'
	3'-GACAT CAGGATGGGTCGAAGTA   ACAGATACTGTGTTGCTAT    TGGTCGTGTCGTGTCAT TGGAC-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 011-011914436
Forward Primer: 5'-GACACAGTACTCAGAGGATCCAAG-3' Tm: 59.038
Reverse Primer: 5'-GCTTGGCTTCTAGGACCAGAC-3' Tm: 59.378
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 130, 140
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr11:11914538-11914590

Rank: 11	Homology Score: 5	RVD Match Score: 2.699	Left Half-Site RVD Score: 5.845	Right Half-Site RVD Score: 4.558
	Genomic Region: Intron	Closest Gene: Tnfaip8
Match Type: R16L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-CCTGG GTTACTGTTGCTCTGAT     CAAACACTGTGACCGA     AGTGAGTTGGGGAGGAA AGGGT-3'
	3'-GGACC CAATGACAACGAGACTA     GTTTGTGACACTGGCT     TCACTCAACCCCTCCTT TCCCA-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 018-045099807
Forward Primer: 5'-GGCTTAGGGTAACATCAGTACCAG-3' Tm: 59.038
Reverse Primer: 5'-AAAAGCAGGCCGGTAGGCAG-3' Tm: 59.508
Expected PCR Product Length: 304
Expected CelI Cleavage Product Sizes: 153, 151
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr18:45099933-45099982

Rank: 12	Homology Score: 10	RVD Match Score: 2.705	Left Half-Site RVD Score: 3.639	Right Half-Site RVD Score: 7.301
	Genomic Region: Intergenic	Closest Gene: Mir532
Match Type: L21R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-TTTTT TTCCTACCCACCCCCAT  GCCTCCCCAGCCTTGGATGAA   ACAGGAACAAAAGTCAA AATTT-3'
	3'-AAAAA AAGGATGGGTGGGGGTA  CGGAGGGGTCGGAACCTACTT   TGTCCTTGTTTTCAGTT TTAAA-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 00X-027254372
Forward Primer: 5'-CCATTATCGCTAGCCTAGCTTACG-3' Tm: 59.038
Reverse Primer: 5'-GACTGGGAATAAGTGGTGTGGTAC-3' Tm: 59.038
Expected PCR Product Length: 301
Expected CelI Cleavage Product Sizes: 167, 134
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chrX:27254510-27254564

Rank: 13	Homology Score: 5	RVD Match Score: 2.714	Left Half-Site RVD Score: 4.296	Right Half-Site RVD Score: 6.355
	Genomic Region: Intergenic	Closest Gene: Pdia6
Match Type: L20R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-CACAA GCCCTTCCCACCTCCAT   GAAGGTATATTGATAAGGTA   ACCACAGTGACATTGAA CTTGA-3'
	3'-GTGTT CGGGAAGGGTGGAGGTA   CTTCCATATAACTATTCCAT   TGGTGTCACTGTAACTT GAACT-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: dom-000581844
Forward Primer: 5'-ATGTGCCTGCAAGGTGGCTG-3' Tm: 59.508
Reverse Primer: 5'-GCTCCAGCCCATATTTGCAGTTC-3' Tm: 59.148
Expected PCR Product Length: 354
Expected CelI Cleavage Product Sizes: 131, 223
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr6_random:581946-581999

Rank: 14	Homology Score: 5	RVD Match Score: 2.73	Left Half-Site RVD Score: 6.826	Right Half-Site RVD Score: 4.081
	Genomic Region: Intergenic	Closest Gene: Arhgap28
Match Type: R17L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-AATGT GTCATTGATGTTTTTGT    ATGTGATGGTGAATGCT     ATGCAGTTGGGGAGGAT GAGTC-3'
	3'-TTACA CAGTAACTACAAAAACA    TACACTACCACTTACGA     TACGTCAACCCCTCCTA CTCAG-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 009-107302997
Forward Primer: 5'-GGCAAACTGACCAGGTCATGTG-3' Tm: 59.258
Reverse Primer: 5'-GAGCTCCTGCTACCAATTATGTGC-3' Tm: 59.038
Expected PCR Product Length: 342
Expected CelI Cleavage Product Sizes: 184, 158
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr9:107303154-107303204

Rank: 15	Homology Score: 13	RVD Match Score: 2.739	Left Half-Site RVD Score: 4.34	Right Half-Site RVD Score: 6.522
	Genomic Region: Intergenic	Closest Gene: Fgfr2
Match Type: L22R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-TTCTG CTCCACCCCAGCTCCAA  AGGAAATGGCTGCCCATAAATC  ACCAGAAAAACACCAAA GTCCC-3'
	3'-AAGAC GAGGTGGGGTCGAGGTT  TCCTTTACCGACGGGTATTTAG  TGGTCTTTTTGTGGTTT CAGGG-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 001-189623849
Forward Primer: 5'-TGCATTCCCACCCCCATACTG-3' Tm: 59.378
Reverse Primer: 5'-CTGAGCACATTCAGGCATCTGG-3' Tm: 59.258
Expected PCR Product Length: 297
Expected CelI Cleavage Product Sizes: 131, 166
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr1:189623950-189624005

Rank: 16	Homology Score: 2	RVD Match Score: 2.74	Left Half-Site RVD Score: 6.222	Right Half-Site RVD Score: 4.551
	Genomic Region: Intergenic	Closest Gene: Usp15
Match Type: R14L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-TTGGA CTCTGTACTGTTCTTGT      TCATTAAGAACCCT      AGGGACTTGGGTAGGAG AAAGG-3'
	3'-AACCT GAGACATGACAAGAACA      AGTAATTCTTGGGA      TCCCTGAACCCATCCTC TTTCC-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 007-062964516
Forward Primer: 5'-TCAAGGCGCAGTCAACCTCC-3' Tm: 59.508
Reverse Primer: 5'-CTGTCAAGGTTGAACAAGGGACTG-3' Tm: 59.038
Expected PCR Product Length: 353
Expected CelI Cleavage Product Sizes: 156, 197
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr7:62964646-62964693

Rank: 17	Homology Score: 17	RVD Match Score: 2.748	Left Half-Site RVD Score: 3.946	Right Half-Site RVD Score: 7.218
	Genomic Region: Intergenic	Closest Gene: Lrfn2
Match Type: L24R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-TCCCC TTCCTGCCCAGCTCCCA TCCCCACCCCCTTCTGAGAACTAA ACAGGAAGCACACGGAA CCTGC-3'
	3'-AGGGG AAGGACGGGTCGAGGGT AGGGGTGGGGGAAGACTCTTGATT TGTCCTTCGTGTGCCTT GGACG-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 009-007190270
Forward Primer: 5'-AGTTGGGCTGGTAGAAATTGCTGG-3' Tm: 59.038
Reverse Primer: 5'-CTGAGTGAAACAAACGGCTCCGA-3' Tm: 59.148
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 133, 137
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr9:7190372-7190429

Rank: 18	Homology Score: 5	RVD Match Score: 2.751	Left Half-Site RVD Score: 4.644	Right Half-Site RVD Score: 5.921
	Genomic Region: Intergenic	Closest Gene: Ccnc
Match Type: R15L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-TCATA CTCAAGGTTGTTCTGAT     GCAAACAACCTCCTC      ATTGTTCTGGGTAGTAA ACAGA-3'
	3'-AGTAT GAGTTCCAACAAGACTA     CGTTTGTTGGAGGAG      TAACAAGACCCATCATT TGTCT-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 005-036123844
Forward Primer: 5'-GGCTGTAGACACTCTTCAGCTTG-3' Tm: 59.148
Reverse Primer: 5'-GTGACCTCTCTGCAGACAGC-3' Tm: 59.508
Expected PCR Product Length: 346
Expected CelI Cleavage Product Sizes: 140, 206
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr5:36123958-36124006

Rank: 19	Homology Score: 8	RVD Match Score: 2.759	Left Half-Site RVD Score: 5.576	Right Half-Site RVD Score: 5.095
	Genomic Region: Intergenic	Closest Gene: Rps29
Match Type: L16R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-GCACA TTCCTCCACATTTCCAT     CTCGAGGAAAGATTGG     ATCAGATCAAGAGTGAA ACTCA-3'
	3'-CGTGT AAGGAGGTGTAAAGGTA     GAGCTCCTTTCTAACC     TAGTCTAGTTCTCACTT TGAGT-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 006-090557385
Forward Primer: 5'-GTCCCCACAGGCATATGATCG-3' Tm: 59.378
Reverse Primer: 5'-GTGCTTCGGGCAGTAGAGCT-3' Tm: 59.508
Expected PCR Product Length: 348
Expected CelI Cleavage Product Sizes: 217, 131
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr6:90557575-90557624

Rank: 20	Homology Score: 5	RVD Match Score: 2.76	Left Half-Site RVD Score: 5.563	Right Half-Site RVD Score: 5.116
	Genomic Region: Intergenic	Closest Gene: Arl5b
Match Type: L16R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-TAATT CTCACTCCCAGCACCAT     ACCCTAAATTTCTAAG     AGCAGAACAATACAGAG ACTTT-3'
	3'-ATTAA GAGTGAGGGTCGTGGTA     TGGGATTTAAAGATTC     TCGTCTTGTTATGTCTC TGAAA-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 017-089324125
Forward Primer: 5'-GGATTTGGGAGAGGAAGGATGAG-3' Tm: 59.148
Reverse Primer: 5'-CATAGTGACACACCCTGAAGTTGC-3' Tm: 59.038
Expected PCR Product Length: 426
Expected CelI Cleavage Product Sizes: 294, 132
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr17:89324392-89324441

Rank: 21	Homology Score: 13	RVD Match Score: 2.76	Left Half-Site RVD Score: 5.076	Right Half-Site RVD Score: 5.706
	Genomic Region: Intergenic	Closest Gene: Wdfy3
Match Type: L23R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-TTTCT TTCAGTCCCAGCTCCAT TTTTGTCCCTGCATTGCCTTTAG  ACAGGAACAACTCTGAG TTGCA-3'
	3'-AAAGA AAGTCAGGGTCGAGGTA AAAACAGGGACGTAACGGAAATC  TGTCCTTGTTGAGACTC AACGT-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 014-008802083
Forward Primer: 5'-TGCCAGGCTCCTGTCTGCAT-3' Tm: 59.508
Reverse Primer: 5'-GCTACCAGGACTCAATGGGG-3' Tm: 59.508
Expected PCR Product Length: 299
Expected CelI Cleavage Product Sizes: 139, 160
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr14:8802192-8802248

Rank: 22	Homology Score: 10	RVD Match Score: 2.765	Left Half-Site RVD Score: 7.226	Right Half-Site RVD Score: 4.042
	Genomic Region: Intergenic	Closest Gene: Glycam1
Match Type: R15L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-TAGCT GTCATGGTAGTTGTGGT     GGTGCACACACATCA      TTGTAGCTGGGCAGGAG TGTTG-3'
	3'-ATCGA CAGTACCATCAACACCA     CCACGTGTGTGTAGT      AACATCGACCCGTCCTC ACAAC-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 007-142568536
Forward Primer: 5'-CCCAAGTTGCTTTTGGTCACGATG-3' Tm: 59.038
Reverse Primer: 5'-ACCCGAGGACTTGAGCCAGA-3' Tm: 59.508
Expected PCR Product Length: 286
Expected CelI Cleavage Product Sizes: 155, 131
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr7:142568665-142568713

Rank: 23	Homology Score: 5	RVD Match Score: 2.771	Left Half-Site RVD Score: 7.418	Right Half-Site RVD Score: 3.963
	Genomic Region: Intergenic	Closest Gene: Mllt1
Match Type: R23L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-TACAG CACAGTGGGGTTCTGGG GGGATGGGTGGGCCGGCAGGAGG  GTGGAGTTGGGAAGGAG AGCAC-3'
	3'-ATGTC GTGTCACCCCAAGACCC CCCTACCCACCCGGCCGTCCTCC  CACCTCAACCCTTCCTC TCGTG-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 0Un-024874250
Forward Primer: 5'-GCCTTCAATTCAGTGAGGAATGGG-3' Tm: 59.038
Reverse Primer: 5'-TACTCAAGTGTGTGCCATGCACC-3' Tm: 59.148
Expected PCR Product Length: 286
Expected CelI Cleavage Product Sizes: 138, 148
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chrUn:24874358-24874414

Rank: 24	Homology Score: 10	RVD Match Score: 2.772	Left Half-Site RVD Score: 5.607	Right Half-Site RVD Score: 5.166
	Genomic Region: Intergenic	Closest Gene: Ttbk2
Match Type: L14R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-GTGTT GCCACGCCCAGCTCCAC      TTTCTAACCTCTAT      ATCAGGACAACACTTAA TAAAT-3'
	3'-CACAA CGGTGCGGGTCGAGGTG      AAAGATTGGAGATA      TAGTCCTGTTGTGAATT ATTTA-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 003-107514838
Forward Primer: 5'-GTTGGCTTAAGGCTGCCAACTTC-3' Tm: 59.148
Reverse Primer: 5'-GTGTGTTGCCTCCTTTTCTACACG-3' Tm: 59.038
Expected PCR Product Length: 440
Expected CelI Cleavage Product Sizes: 234, 206
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr3:107515046-107515093

Rank: 25	Homology Score: 5	RVD Match Score: 2.775	Left Half-Site RVD Score: 6.076	Right Half-Site RVD Score: 4.891
	Genomic Region: Intergenic	Closest Gene: Fyn
Match Type: R14L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-GGAGA TCCAGTTTTCTTCTTGT      CAGGGGTGGGTGGG      ATGGGGTGGGGTAGGTA GGAGA-3'
	3'-CCTCT AGGTCAAAAGAAGAACA      GTCCCCACCCACCC      TACCCCACCCCATCCAT CCTCT-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. Warning! The sequence of this region of the genome is poorly mapped. 
Primer Index: 020-043706993
Forward Primer: 5'-GCAGAAATGGATAGTTTGTGGGGG-3' Tm: 59.038
Reverse Primer: 5'-CCGCCAAGCCCTTTTTAAGTACTG-3' Tm: 59.038
Expected PCR Product Length: 378
Expected CelI Cleavage Product Sizes: 248, 130
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr20:43707215-43707262

Rank: 26	Homology Score: 5	RVD Match Score: 2.775	Left Half-Site RVD Score: 6.199	Right Half-Site RVD Score: 4.801
	Genomic Region: Intron	Closest Gene: Gxylt1
Match Type: R16L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-AAGTA GTAATGGTTGTTCTGAT     TGTCAAACTGACATAC     AGTGAGCTGGGAAGGAG GTCTC-3'
	3'-TTCAT CATTACCAACAAGACTA     ACAGTTTGACTGTATG     TCACTCGACCCTTCCTC CAGAG-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 007-131749648
Forward Primer: 5'-GGCTTCCTACCCAGCTTTATTTCC-3' Tm: 59.038
Reverse Primer: 5'-GGACTCTGAGTTGCTGAGCC-3' Tm: 59.508
Expected PCR Product Length: 273
Expected CelI Cleavage Product Sizes: 143, 130
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr7:131749764-131749813

Rank: 27	Homology Score: 8	RVD Match Score: 2.775	Left Half-Site RVD Score: 6.431	Right Half-Site RVD Score: 4.313
	Genomic Region: Intergenic	Closest Gene: Odz3
Match Type: L17R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-TGTGC TTCCAGAGTAGCTCCAT    GAACACCTGTGATGTCA     AGTAGAACAACAGTGAC TTCAA-3'
	3'-ACACG AAGGTCTCATCGAGGTA    CTTGTGGACACTACAGT     TCATCTTGTTGTCACTG AAGTT-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 016-044835388
Forward Primer: 5'-GTCCCATAACTGGGGCTAGG-3' Tm: 59.508
Reverse Primer: 5'-CCCTCTTAGGTCCATTCCACTAG-3' Tm: 59.148
Expected PCR Product Length: 404
Expected CelI Cleavage Product Sizes: 222, 182
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr16:44835583-44835633

Rank: 28	Homology Score: 10	RVD Match Score: 2.782	Left Half-Site RVD Score: 5.384	Right Half-Site RVD Score: 5.512
	Genomic Region: Promoter	Closest Gene: Zfp830
Match Type: L24R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-ACCTC TGCCTGCCTAGCTCCAA AGCTAGTACCCGCATGTCTGCCAC ATCAAAACAATACTGTC TGCGA-3'
	3'-TGGAG ACGGACGGATCGAGGTT TCGATCATGGGCGTACAGACGGTG TAGTTTTGTTATGACAG ACGCT-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 010-070982895
Forward Primer: 5'-CAGGGTGTGTCTTTCCCAAGATAC-3' Tm: 59.038
Reverse Primer: 5'-GGGTCTCAGGAAACGTTGTGTTG-3' Tm: 59.148
Expected PCR Product Length: 366
Expected CelI Cleavage Product Sizes: 221, 145
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr10:70983085-70983142

Rank: 29	Homology Score: 5	RVD Match Score: 2.784	Left Half-Site RVD Score: 5.832	Right Half-Site RVD Score: 5.142
	Genomic Region: Intergenic	Closest Gene: Pdlim1
Match Type: R24L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-CCTCT GTCAGTGTTAACCAGGT AGAGAGGGACAACTATTACCTCAG ACGGGGCTGGGCTGGAG GAGGT-3'
	3'-GGAGA CAGTCACAATTGGTCCA TCTCTCCCTGTTGATAATGGAGTC TGCCCCGACCCGACCTC CTCCA-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 001-245506315
Forward Primer: 5'-AGGTCAGAGGTGAGGCAGCA-3' Tm: 59.508
Reverse Primer: 5'-TCACTGCATGTCCATGCGCAAC-3' Tm: 59.258
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 137, 133
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr1:245506421-245506478

Rank: 30	Homology Score: 5	RVD Match Score: 2.786	Left Half-Site RVD Score: 5.664	Right Half-Site RVD Score: 5.212
	Genomic Region: Intron	Closest Gene: Prickle2
Match Type: L13R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-CCTGT TTCCTTCCCAAATCCCC      TGACAAGACATAG       AGCAGAAGAACATTTAA AATTA-3'
	3'-GGACA AAGGAAGGGTTTAGGGG      ACTGTTCTGTATC       TCGTCTTCTTGTAAATT TTAAT-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 004-126653383
Forward Primer: 5'-CCATGTGACCTTTGGACTACATGC-3' Tm: 59.038
Reverse Primer: 5'-GAGAGCCACTGACTACCATAACAC-3' Tm: 59.038
Expected PCR Product Length: 327
Expected CelI Cleavage Product Sizes: 145, 182
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr4:126653503-126653549

Rank: 31	Homology Score: 5	RVD Match Score: 2.79	Left Half-Site RVD Score: 5.049	Right Half-Site RVD Score: 5.841
	Genomic Region: Intron	Closest Gene: Il1rapl1
Match Type: R17L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-TGTGA CCCAGAATTGTTCTGGT    CTGAAAGAACTACAGGG     ATGGAAATGGAGAGGAG CCTGA-3'
	3'-ACACT GGGTCTTAACAAGACCA    GACTTTCTTGATGTCCC     TACCTTTACCTCTCCTC GGACT-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 00X-074124866
Forward Primer: 5'-AATGCCCTACAGTGGGGAGAG-3' Tm: 59.378
Reverse Primer: 5'-CAAAGGACAGTCATGATAGGTCCC-3' Tm: 59.038
Expected PCR Product Length: 282
Expected CelI Cleavage Product Sizes: 130, 152
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chrX:74124969-74125019

Rank: 32	Homology Score: 5	RVD Match Score: 2.792	Left Half-Site RVD Score: 7.557	Right Half-Site RVD Score: 4.007
	Genomic Region: Intergenic	Closest Gene: Blvrb
Match Type: R21L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-ATGGG CTCTGTGTGGTACTGGG  TGAGATGGATGTATTTTCACA   GTGGAGTTGGGCGGGAT TCAGA-3'
	3'-TACCC GAGACACACCATGACCC  ACTCTACCTACATAAAAGTGT   CACCTCAACCCGCCCTA AGTCT-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 001-082505606
Forward Primer: 5'-TAAGGTGGGGGTGTGGCTGA-3' Tm: 59.508
Reverse Primer: 5'-CCAACGCCAACTACAACCTGC-3' Tm: 59.378
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 138, 132
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr1:82505715-82505769

Rank: 33	Homology Score: 10	RVD Match Score: 2.794	Left Half-Site RVD Score: 4.36	Right Half-Site RVD Score: 7.012
	Genomic Region: Intron	Closest Gene: Rnf4
Match Type: L19R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-GTAGA TCCCTCCCCAGCTCCCT   TGTCAGGACCTAGCCAGGT    ACATGATCAACATTCAA GAAAA-3'
	3'-CATCT AGGGAGGGGTCGAGGGA   ACAGTCCTGGATCGGTCCA    TGTACTAGTTGTAAGTT CTTTT-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 014-082098705
Forward Primer: 5'-AGCCAAGGAAGAAACCTGTGCAGC-3' Tm: 60.908
Reverse Primer: 5'-CTGTGGATGGCTAGAGTGTACTCA-3' Tm: 59.038
Expected PCR Product Length: 300
Expected CelI Cleavage Product Sizes: 130, 170
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr14:82098807-82098859

Rank: 34	Homology Score: 10	RVD Match Score: 2.794	Left Half-Site RVD Score: 7.385	Right Half-Site RVD Score: 4.124
	Genomic Region: Intergenic	Closest Gene: Pard3
Match Type: R12L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-TTTGC TTTTTTATTGTTCTGGA       AGTTAAGTTTGA       ATGGGGCTTGGTAGGAA ATGAT-3'
	3'-AAACG AAAAAATAACAAGACCT       TCAATTCAAACT       TACCCCGAACCATCCTT TACTA-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 019-057896612
Forward Primer: 5'-CCCAGTGCTGGGGTTACAAACA-3' Tm: 59.258
Reverse Primer: 5'-TCTTAGCCAGGAATGAGGCTGC-3' Tm: 59.258
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 138, 132
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr19:57896725-57896770

Rank: 35	Homology Score: 13	RVD Match Score: 2.8	Left Half-Site RVD Score: 6.458	Right Half-Site RVD Score: 4.782
	Genomic Region: Intron	Closest Gene: Usp30
Match Type: R24L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-CCCAG TTCAGTGTTGGGATGGT GGCAGGATACACAGAGGCTTGGCA GTGCAGCTGGGGAGGAT GTGTG-3'
	3'-GGGTC AAGTCACAACCCTACCA CCGTCCTATGTGTCTCCGAACCGT CACGTCGACCCCTCCTA CACAC-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 012-043552977
Forward Primer: 5'-CACCCACTTCATGTTGGAAACGC-3' Tm: 59.148
Reverse Primer: 5'-TTCCAAACCCTCTGCCCTGC-3' Tm: 59.508
Expected PCR Product Length: 278
Expected CelI Cleavage Product Sizes: 131, 147
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr12:43553077-43553134

Rank: 36	Homology Score: 5	RVD Match Score: 2.801	Left Half-Site RVD Score: 6.947	Right Half-Site RVD Score: 4.449
	Genomic Region: Intergenic	Closest Gene: Clcn7
Match Type: R20L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-GGATA TACAGAGTAGTCCTGCT   TTTAAACCTCACAGGTTCTG   ATGGAGCTGGCTAGGTT GTCCT-3'
	3'-CCTAT ATGTCTCATCAGGACGA   AAATTTGGAGTGTCCAAGAC   TACCTCGACCGATCCAA CAGGA-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 010-014405848
Forward Primer: 5'-CCTGGTGTTCAGCCTTGGGT-3' Tm: 59.508
Reverse Primer: 5'-TGCTGTCACCTGACCTCAGAGA-3' Tm: 59.258
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 133, 137
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr10:14405952-14406005

Rank: 37	Homology Score: 10	RVD Match Score: 2.802	Left Half-Site RVD Score: 6.686	Right Half-Site RVD Score: 4.635
	Genomic Region: Intergenic	Closest Gene: Pde4b
Match Type: R15L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-TTTCG TCCATTATTGTCATGGT     GTCATGCAAGGTGAC      ATGGTGCTGGGGAGGCA GCTGA-3'
	3'-AAAGC AGGTAATAACAGTACCA     CAGTACGTTCCACTG      TACCACGACCCCTCCGT CGACT-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 005-123636672
Forward Primer: 5'-CTCTGAGTGACACGAATTAGTGGG-3' Tm: 59.038
Reverse Primer: 5'-GGAGTGTGTCACTAAGGCTGG-3' Tm: 59.378
Expected PCR Product Length: 350
Expected CelI Cleavage Product Sizes: 218, 132
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr5:123636864-123636912

Rank: 38	Homology Score: 5	RVD Match Score: 2.803	Left Half-Site RVD Score: 5.862	Right Half-Site RVD Score: 5.251
	Genomic Region: Intergenic	Closest Gene: Tasp1
Match Type: R14L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-TGTCT TTCAGTTTTGTTGTACT      GAGAGAGAAAGAGA      ATGAAGTTGGGTGGGTA GGAGG-3'
	3'-ACAGA AAGTCAAAACAACATGA      CTCTCTCTTTCTCT      TACTTCAACCCACCCAT CCTCC-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 003-128243778
Forward Primer: 5'-CAACCACACTGCAGACCAGG-3' Tm: 59.508
Reverse Primer: 5'-GCCATGGATCTGATATTGGTGGCT-3' Tm: 59.038
Expected PCR Product Length: 308
Expected CelI Cleavage Product Sizes: 131, 177
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr3:128243883-128243930

Rank: 39	Homology Score: 2	RVD Match Score: 2.804	Left Half-Site RVD Score: 5.348	Right Half-Site RVD Score: 5.752
	Genomic Region: Intron	Closest Gene: Muc15
Match Type: L16R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-TATTG CTCCTACCCAGGTACAA     GAAGATGTAAAAACAC     AGGAGAAATACACTGAT TTTTT-3'
	3'-ATAAC GAGGATGGGTCCATGTT     CTTCTACATTTTTGTG     TCCTCTTTATGTGACTA AAAAA-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 003-096182235
Forward Primer: 5'-CTGTTCATCCTCCCTGTGGAAG-3' Tm: 59.258
Reverse Primer: 5'-TTGCGGCATGCTTGAGTTTGCCC-3' Tm: 61.088
Expected PCR Product Length: 432
Expected CelI Cleavage Product Sizes: 300, 132
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr3:96182508-96182557

Rank: 40	Homology Score: 5	RVD Match Score: 2.805	Left Half-Site RVD Score: 5.898	Right Half-Site RVD Score: 5.237
	Genomic Region: Intergenic	Closest Gene: Micu1
Match Type: R24L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-TCATG ACCTGTCTTGTTCTGAT TCCTTGTCTCCTAAGAGGAAAGGA CTGGAGCTTTGCAGGAG GATAG-3'
	3'-AGTAC TGGACAGAACAAGACTA AGGAACAGAGGATTCTCCTTTCCT GACCTCGAAACGTCCTC CTATC-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: dom-004607360
Forward Primer: 5'-GGCCAACTCTTGAGAGAACAAGTG-3' Tm: 59.038
Reverse Primer: 5'-CTATCTTGTATTCGCAGCGCCTC-3' Tm: 59.148
Expected PCR Product Length: 285
Expected CelI Cleavage Product Sizes: 155, 130
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chrUn_random:4607484-4607541

Rank: 41	Homology Score: 13	RVD Match Score: 2.805	Left Half-Site RVD Score: 6.508	Right Half-Site RVD Score: 4.782
	Genomic Region: Intergenic	Closest Gene: Atpbd4
Match Type: R15L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-CATAA TTCAATGTTGTTTGGGC     TTTTTTTTTTTTTTT      TTGGAGCTGGGGACGAA CCCAG-3'
	3'-GTATT AAGTTACAACAAACCCG     AAAAAAAAAAAAAAA      AACCTCGACCCCTGCTT GGGTC-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 003-100952790
Forward Primer: 5'-GCCATGAAGAAGACATGCAGGAG-3' Tm: 59.148
Reverse Primer: 5'-GGATCACTTGAAATGTGGAGCTTCCTAC-3' Tm: 60.298
Expected PCR Product Length: 405
Expected CelI Cleavage Product Sizes: 218, 187
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr3:100952982-100953030

Rank: 42	Homology Score: 2	RVD Match Score: 2.808	Left Half-Site RVD Score: 4.762	Right Half-Site RVD Score: 6.241
	Genomic Region: Intergenic	Closest Gene: Chst11
Match Type: R24L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-ATGTA GCCAGTATTATTCTGCT TCTCCTTTATGAACGAGTTGCTTG CTGGAGGTGGCCAAGAT ATATG-3'
	3'-TACAT CGGTCATAATAAGACGA AGAGGAAATACTTGCTCAACGAAC GACCTCCACCGGTTCTA TATAC-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 007-022951295
Forward Primer: 5'-TGAGACCTCCTTTTCCTTCTCCTG-3' Tm: 59.038
Reverse Primer: 5'-ATATGCCCCTGCAAGGAGCC-3' Tm: 59.508
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 134, 136
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr7:22951398-22951455

Rank: 43	Homology Score: 5	RVD Match Score: 2.809	Left Half-Site RVD Score: 5.089	Right Half-Site RVD Score: 6.127
	Genomic Region: Intergenic	Closest Gene: Bnc1
Match Type: L21R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-CGGCT CCCCTGTCCAGCTCCAC  TTGCAGACTCTCTTTGCTCCC   ACCAGAACTCTGCTGAG TGACC-3'
	3'-GCCGA GGGGACAGGTCGAGGTG  AACGTCTGAGAGAAACGAGGG   TGGTCTTGAGACGACTC ACTGG-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 001-138291107
Forward Primer: 5'-TATCTATGTGGCAGGCTGGGTAC-3' Tm: 59.148
Reverse Primer: 5'-GTGCAGTGTCATACCCGGGTT-3' Tm: 59.378
Expected PCR Product Length: 279
Expected CelI Cleavage Product Sizes: 130, 149
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr1:138291208-138291262

Rank: 44	Homology Score: 2	RVD Match Score: 2.814	Left Half-Site RVD Score: 7.048	Right Half-Site RVD Score: 4.469
	Genomic Region: Intergenic	Closest Gene: Cdk14
Match Type: R21L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-AGGGT TACTGTGCTATACTGGT  CATCATTGTAGTCCACAGGCA   TTAGAGTTGGGTAGGAC TGTTG-3'
	3'-TCCCA ATGACACGATATGACCA  GTAGTAACATCAGGTGTCCGT   AATCTCAACCCATCCTG ACAAC-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 004-025342811
Forward Primer: 5'-CCAGTACCAGGTATAACTTCCCTC-3' Tm: 59.038
Reverse Primer: 5'-GGACCCAGAGACACTGAGCT-3' Tm: 59.508
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 133, 137
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr4:25342915-25342969

Rank: 45	Homology Score: 5	RVD Match Score: 2.814	Left Half-Site RVD Score: 4.825	Right Half-Site RVD Score: 6.534
	Genomic Region: Intron	Closest Gene: Gli3
Match Type: L17R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-AGCCA AACCTGCCAAGCTCCAC    AGCTGGTCGTGGGGAGG     AGGGGAACAAAGCTGAA TGGGG-3'
	3'-TCGGT TTGGACGGTTCGAGGTG    TCGACCAGCACCCCTCC     TCCCCTTGTTTCGACTT ACCCC-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 017-057702366
Forward Primer: 5'-CGGCACATAATCCTGCCTCTC-3' Tm: 59.378
Reverse Primer: 5'-GTCTAGTGATCCCTCCCTACTTTG-3' Tm: 59.038
Expected PCR Product Length: 294
Expected CelI Cleavage Product Sizes: 130, 164
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr17:57702469-57702519

Rank: 46	Homology Score: 5	RVD Match Score: 2.816	Left Half-Site RVD Score: 3.971	Right Half-Site RVD Score: 7.859
	Genomic Region: Intergenic	Closest Gene: Caln1
Match Type: L18R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-TCTCT CTCCTCCCCACCCCCAT    GCACAGCATGTATGCACA    CCCAGAAAAAAAATAAA TACAT-3'
	3'-AGAGA GAGGAGGGGTGGGGGTA    CGTGTCGTACATACGTGT    GGGTCTTTTTTTTATTT ATGTA-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 012-027291771
Forward Primer: 5'-TCCTAAGGGTGGCCTTTTGACCTC-3' Tm: 60.908
Reverse Primer: 5'-CAGCCTCAGCTCACAGAGATC-3' Tm: 59.378
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 134, 136
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr12:27291877-27291928

Rank: 47	Homology Score: 2	RVD Match Score: 2.821	Left Half-Site RVD Score: 6.244	Right Half-Site RVD Score: 5.084
	Genomic Region: Intron	Closest Gene: Grin2a
Match Type: R23L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-TGCCA GGCAGTGCTGTTATGAT CGGCCTGGAGGCTTCCTAGAACT  CTGGAGCTGGGTTGGGC CTCCC-3'
	3'-ACGGT CCGTCACGACAATACTA GCCGGACCTCCGAAGGATCTTGA  GACCTCGACCCAACCCG GAGGG-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 010-005637007
Forward Primer: 5'-ACGAATGCACAGAAACAGCTGTGG-3' Tm: 59.038
Reverse Primer: 5'-GCTAAGAAGCAGACGCTGTGCA-3' Tm: 59.258
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 134, 136
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr10:5637111-5637167

Rank: 48	Homology Score: 17	RVD Match Score: 2.822	Left Half-Site RVD Score: 5.219	Right Half-Site RVD Score: 5.929
	Genomic Region: Intergenic	Closest Gene: RGD1559903
Match Type: R16L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-TTCCA TGCAGTGTTGTTCTGTT     GTCAGTAATGGATGCC     AGAGAGTTGGGATGGAA TGAGA-3'
	3'-AAGGT ACGTCACAACAAGACAA     CAGTCATTACCTACGG     TCTCTCAACCCTACCTT ACTCT-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 020-016460972
Forward Primer: 5'-GTCAAGGACGAAGTCATCTGGAG-3' Tm: 59.148
Reverse Primer: 5'-GACAAGCTGACTGCTGCTAGAG-3' Tm: 59.258
Expected PCR Product Length: 340
Expected CelI Cleavage Product Sizes: 144, 196
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr20:16461089-16461138

Rank: 49	Homology Score: 5	RVD Match Score: 2.823	Left Half-Site RVD Score: 5.049	Right Half-Site RVD Score: 6.09
	Genomic Region: Intron	Closest Gene: Pitpnm2
Match Type: R21L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-CCCTG ATCAGTGCAGTTCTAGT  GGACCAGGGAGGGGTGTGCCA   AGTGTGTTGGACAGGAA CAGAA-3'
	3'-GGGAC TAGTCACGTCAAGATCA  CCTGGTCCCTCCCCACACGGT   TCACACAACCTGTCCTT GTCTT-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 012-033556886
Forward Primer: 5'-TCTGTCTGCAAGCTCCAGGG-3' Tm: 59.508
Reverse Primer: 5'-AACTCTGGCCTCCTTGGTCC-3' Tm: 59.508
Expected PCR Product Length: 277
Expected CelI Cleavage Product Sizes: 139, 138
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr12:33556996-33557050

Rank: 50	Homology Score: 5	RVD Match Score: 2.825	Left Half-Site RVD Score: 5.484	Right Half-Site RVD Score: 5.722
	Genomic Region: Intergenic	Closest Gene: Gnaz
Match Type: R16L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-TTAGA CTCTGTATTGCTCTGGT     AAAACATATGACCAAA     AGTAAGTTGGGGAGGAA GGGGT-3'
	3'-AATCT GAGACATAACGAGACCA     TTTTGTATACTGGTTT     TCATTCAACCCCTCCTT CCCCA-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 020-014621051
Forward Primer: 5'-GCCTGTGGTTGGTACATGTACCT-3' Tm: 59.148
Reverse Primer: 5'-GAGCCGTGATTCTCCATGGATTC-3' Tm: 59.148
Expected PCR Product Length: 366
Expected CelI Cleavage Product Sizes: 236, 130
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr20:14621260-14621309

Rank: 51	Homology Score: 2	RVD Match Score: 2.828	Left Half-Site RVD Score: 4.862	Right Half-Site RVD Score: 6.614
	Genomic Region: Intron	Closest Gene: Mocs1
Match Type: L20R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-GCTTA GTCCTACCCATCCACAT   CGAGGCTGCTGTTTCAAGCA   ACCAGGGCATTGCTGAA CACCT-3'
	3'-CGAAT CAGGATGGGTAGGTGTA   GCTCCGACGACAAAGTTCGT   TGGTCCCGTAACGACTT GTGGA-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 009-006766208
Forward Primer: 5'-GCTCTGGAGAAAGGTTGCCATG-3' Tm: 59.258
Reverse Primer: 5'-CTGGAGAAGCTGCCAGAGGA-3' Tm: 59.508
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 135, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr9:6766314-6766367

Rank: 52	Homology Score: 2	RVD Match Score: 2.829	Left Half-Site RVD Score: 5.5	Right Half-Site RVD Score: 5.737
	Genomic Region: Intergenic	Closest Gene: LOC500827
Match Type: R13L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-TCTGA TTCAATAATCTTCTGTT      TTATAAAGTCCTT       ATGGATCTTGGGAAGAG GTAGT-3'
	3'-AGACT AAGTTATTAGAAGACAA      AATATTTCAGGAA       TACCTAGAACCCTTCTC CATCA-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 007-038298476
Forward Primer: 5'-AAAAGCATGAGCTCCTCAGGCTGG-3' Tm: 60.908
Reverse Primer: 5'-GAAGAGGAAACTGTGGTCAGGTTG-3' Tm: 59.038
Expected PCR Product Length: 370
Expected CelI Cleavage Product Sizes: 130, 240
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr7:38298581-38298627

Rank: 53	Homology Score: 13	RVD Match Score: 2.829	Left Half-Site RVD Score: 4.456	Right Half-Site RVD Score: 7.218
	Genomic Region: Intron	Closest Gene: Sars
Match Type: L22R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-GTAAG TACCTCCCCAGCTCAAT  TCCACTCACACAACCACAGATA  ACCAGGCCAAAACAGAA GTACC-3'
	3'-CATTC ATGGAGGGGTCGAGTTA  AGGTGAGTGTGTTGGTGTCTAT  TGGTCCGGTTTTGTCTT CATGG-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 002-204001007
Forward Primer: 5'-AATTTATGGGCTCTGTGCCGTGC-3' Tm: 59.148
Reverse Primer: 5'-CCCAGTGCTAAGTTGAGTGTGGC-3' Tm: 61.088
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 135, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr2:204001112-204001167

Rank: 54	Homology Score: 5	RVD Match Score: 2.831	Left Half-Site RVD Score: 5.503	Right Half-Site RVD Score: 5.796
	Genomic Region: Intergenic	Closest Gene: Csmd1
Match Type: L15R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-AGTCA TTCCTGCTCACCCCCAC     ACTGACAGAAAGAAA      ACCTGAAGAGGACTGAG TAAAG-3'
	3'-TCAGT AAGGACGAGTGGGGGTG     TGACTGTCTTTCTTT      TGGACTTCTCCTGACTC ATTTC-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 016-077159880
Forward Primer: 5'-CTGTGGAGTTTGTGCAGGAATGCT-3' Tm: 59.038
Reverse Primer: 5'-GGGGCAGTGTGTGATCAAAAGG-3' Tm: 59.258
Expected PCR Product Length: 360
Expected CelI Cleavage Product Sizes: 170, 190
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr16:77160024-77160072

Rank: 55	Homology Score: 2	RVD Match Score: 2.832	Left Half-Site RVD Score: 6.722	Right Half-Site RVD Score: 4.81
	Genomic Region: Intergenic	Closest Gene: Gdnf
Match Type: R15L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-AGGAG GTAAGTGTTGCTATGTT     AAGTAAGACCCTGGC      AGGGGGCTGGGTGGGAG CAGGT-3'
	3'-TCCTC CATTCACAACGATACAA     TTCATTCTGGGACCG      TCCCCCGACCCACCCTC GTCCA-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 002-057295241
Forward Primer: 5'-GGCACTCTTGGGATGAGTGTAG-3' Tm: 59.258
Reverse Primer: 5'-GCTCTACTCTCATCTGCTGATTAATCTCTG-3' Tm: 60.058
Expected PCR Product Length: 396
Expected CelI Cleavage Product Sizes: 150, 246
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr2:57295365-57295413

Rank: 56	Homology Score: 2	RVD Match Score: 2.834	Left Half-Site RVD Score: 6.029	Right Half-Site RVD Score: 5.34
	Genomic Region: Intron	Closest Gene: Prex1
Match Type: R14L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-GAACC TTAAGTACTATCCTGGT      AGAAGCGGTTTGGG      AGGGGTTTGGGGAGGAA GTCCA-3'
	3'-CTTGG AATTCATGATAGGACCA      TCTTCGCCAAACCC      TCCCCAAACCCCTCCTT CAGGT-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 003-157836620
Forward Primer: 5'-GGCAAAGCTGCCTGTCACCA-3' Tm: 59.508
Reverse Primer: 5'-GCTTCAGGCACAGCTGGATC-3' Tm: 59.508
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 136, 134
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr3:157836730-157836777

Rank: 57	Homology Score: 5	RVD Match Score: 2.835	Left Half-Site RVD Score: 6.244	Right Half-Site RVD Score: 5.184
	Genomic Region: Intron	Closest Gene: Fbxw11
Match Type: R14L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-TCCTA GTCAGGGTTACTATGGT      TATAATGAAACATC      ATGAAGTTAGGGAGGAA AGGGT-3'
	3'-AGGAT CAGTCCCAATGATACCA      ATATTACTTTGTAG      TACTTCAATCCCTCCTT TCCCA-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 010-017513398
Forward Primer: 5'-CTCATGCTTGTGCAGCAAGCACT-3' Tm: 59.148
Reverse Primer: 5'-TTGATGGGGCAGGGCATCAG-3' Tm: 59.508
Expected PCR Product Length: 279
Expected CelI Cleavage Product Sizes: 144, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr10:17513516-17513563

Rank: 58	Homology Score: 13	RVD Match Score: 2.836	Left Half-Site RVD Score: 6.116	Right Half-Site RVD Score: 5.124
	Genomic Region: Intron	Closest Gene: Ppm1l
Match Type: L12R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-ACTCT TTCCTCCCAAGCTGCTT       TTGGGCGGTTTT       ACCACAATAACAGTGAA GCAAA-3'
	3'-TGAGA AAGGAGGGTTCGACGAA       AACCCGCCAAAA       TGGTGTTATTGTCACTT CGTTT-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 002-159432579
Forward Primer: 5'-GCTAAATCCCCAACCCTGCAG-3' Tm: 59.378
Reverse Primer: 5'-GTGTTTACTTGCTCAAACACCCGG-3' Tm: 59.038
Expected PCR Product Length: 284
Expected CelI Cleavage Product Sizes: 154, 130
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr2:159432708-159432753

Rank: 59	Homology Score: 2	RVD Match Score: 2.836	Left Half-Site RVD Score: 6.856	Right Half-Site RVD Score: 4.745
	Genomic Region: Intergenic	Closest Gene: Atg14
Match Type: R20L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-GTGGG GTCTGTGGGATTCTGGT   CTAATTGGGAGTGCCTGGGG   GTGGTGTTGGGAAGGAG GCCTT-3'
	3'-CACCC CAGACACCCTAAGACCA   GATTAACCCTCACGGACCCC   CACCACAACCCTTCCTC CGGAA-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 015-023818731
Forward Primer: 5'-CACTAGGCCATGTGTAGTAGCTG-3' Tm: 59.148
Reverse Primer: 5'-GTCCACCCCATATAAAGTGTACGC-3' Tm: 59.038
Expected PCR Product Length: 271
Expected CelI Cleavage Product Sizes: 141, 130
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr15:23818843-23818896

Rank: 60	Homology Score: 2	RVD Match Score: 2.837	Left Half-Site RVD Score: 5.287	Right Half-Site RVD Score: 6.127
	Genomic Region: Intergenic	Closest Gene: Vps39
Match Type: L19R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-TCCCA GTCCCACACAGTTCCAT   ACCCATTTCCCTACTCACC    ACCAGGACACCATTGGT GATGA-3'
	3'-AGGGT CAGGGTGTGTCAAGGTA   TGGGTAAAGGGATGAGTGG    TGGTCCTGTGGTAACCA CTACT-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 003-106797759
Forward Primer: 5'-GCTTCTTGTTCTTCATTCCCCTGG-3' Tm: 59.038
Reverse Primer: 5'-TCACACTTCTCAGGCTGCTTTGG-3' Tm: 59.148
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 134, 136
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr3:106797865-106797917

Rank: 61	Homology Score: 2	RVD Match Score: 2.84	Left Half-Site RVD Score: 5.348	Right Half-Site RVD Score: 6.076
	Genomic Region: Intergenic	Closest Gene: Grin3a
Match Type: L18R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-GAATG ATCCTAACCAGCAGCAT    AACAGAAAATCTGGAAGC    TCAAGAACAAAAGTGAG CAAAC-3'
	3'-CTTAC TAGGATTGGTCGTCGTA    TTGTCTTTTAGACCTTCG    AGTTCTTGTTTTCACTC GTTTG-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 005-067767253
Forward Primer: 5'-CCCAATAATTCTTACCCTCTTGGGGGA-3' Tm: 60.438
Reverse Primer: 5'-GTGGCCCTTAACTGATCTGGCT-3' Tm: 59.258
Expected PCR Product Length: 414
Expected CelI Cleavage Product Sizes: 253, 161
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr5:67767478-67767529

Rank: 62	Homology Score: 2	RVD Match Score: 2.84	Left Half-Site RVD Score: 5.348	Right Half-Site RVD Score: 6.076
	Genomic Region: Intergenic	Closest Gene: LOC100363289
Match Type: L18R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-GAATG ATCCTAACCAGCAGCAT    AACAGAAAATCTGGAAGC    TCAAGAACAAAAGTGAG CAAAC-3'
	3'-CTTAC TAGGATTGGTCGTCGTA    TTGTCTTTTAGACCTTCG    AGTTCTTGTTTTCACTC GTTTG-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Warning! Primer pair was generated using suboptimal parameters. Warning! The sequence of this region of the genome is poorly mapped. 
Primer Index: 008-084548726
Forward Primer: 5'-TTCTATTATTTCATGAAAATGAAGGCATGGCATGGTG-3' Tm: 59.428
Reverse Primer: 5'-CCTTATGGGTGTTCCCCTCC-3' Tm: 59.508
Expected PCR Product Length: 335
Expected CelI Cleavage Product Sizes: 131, 204
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr8:84548829-84548880

Rank: 63	Homology Score: 5	RVD Match Score: 2.846	Left Half-Site RVD Score: 5.259	Right Half-Site RVD Score: 6.239
	Genomic Region: Intergenic	Closest Gene: Mfhas1
Match Type: L14R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-ACCCT TTCCTCCCCAGCACACT      TTGGTCATGATGTT      AGCACAGCAACAGTGAC CCCAA-3'
	3'-TGGGA AAGGAGGGGTCGTGTGA      AACCAGTACTACAA      TCGTGTCGTTGTCACTG GGGTT-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 016-060358235
Forward Primer: 5'-CTCTGCATCAGTCCTGCCTTC-3' Tm: 59.378
Reverse Primer: 5'-GGGCTACAGAGTAAGACTGTGTCA-3' Tm: 59.038
Expected PCR Product Length: 302
Expected CelI Cleavage Product Sizes: 133, 169
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr16:60358342-60358389

Rank: 64	Homology Score: 2	RVD Match Score: 2.847	Left Half-Site RVD Score: 5.902	Right Half-Site RVD Score: 5.463
	Genomic Region: Intergenic	Closest Gene: Mecom
Match Type: L21R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-CCCAA CACCTGCCAAGCACCAG  AATATCAGATGGGCACTCTGG   ACCAGAGGAATGCTGAT AGTGT-3'
	3'-GGGTT GTGGACGGTTCGTGGTC  TTATAGTCTACCCGTGAGACC   TGGTCTCCTTACGACTA TCACA-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 002-117980578
Forward Primer: 5'-CAACACACACACACAGGTTTCCTC-3' Tm: 59.038
Reverse Primer: 5'-CTCTACTGTGTTTGGAGGAGGATG-3' Tm: 59.038
Expected PCR Product Length: 312
Expected CelI Cleavage Product Sizes: 143, 169
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr2:117980692-117980746

Rank: 65	Homology Score: 5	RVD Match Score: 2.848	Left Half-Site RVD Score: 7.493	Right Half-Site RVD Score: 4.397
	Genomic Region: Intergenic	Closest Gene: Myo5b
Match Type: R12L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-CCCTG TGCAGTGTGGATCTTCT       TTGGTGTAGATA       AAGGAGTTGGGAAGGAG CTGGA-3'
	3'-GGGAC ACGTCACACCTAGAAGA       AACCACATCTAT       TTCCTCAACCCTTCCTC GACCT-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 018-071340665
Forward Primer: 5'-CCCCCATCCCCTTTTACAGTTC-3' Tm: 59.258
Reverse Primer: 5'-GTAGAGGTCCCTCCTTGAGATTC-3' Tm: 59.148
Expected PCR Product Length: 301
Expected CelI Cleavage Product Sizes: 148, 153
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr18:71340788-71340833

Rank: 66	Homology Score: 5	RVD Match Score: 2.849	Left Half-Site RVD Score: 5.944	Right Half-Site RVD Score: 5.428
	Genomic Region: Intergenic	Closest Gene: Lypd1
Match Type: L21R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-AGCTT TTCCTTCACAGCATCAT  GTGTCCATGACCACATAGGCA   AGCAGAACAAGAGTGTG CTTTG-3'
	3'-TCGAA AAGGAAGTGTCGTAGTA  CACAGGTACTGGTGTATCCGT   TCGTCTTGTTCTCACAC GAAAC-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 013-038906135
Forward Primer: 5'-GCCCCACAGCTATCTCTTTTTGGA-3' Tm: 59.038
Reverse Primer: 5'-CTGGAGACAGCTTTCAAATCACCC-3' Tm: 59.038
Expected PCR Product Length: 436
Expected CelI Cleavage Product Sizes: 298, 138
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr13:38906404-38906458

Rank: 67	Homology Score: 2	RVD Match Score: 2.849	Left Half-Site RVD Score: 4.511	Right Half-Site RVD Score: 6.812
	Genomic Region: Intron	Closest Gene: Utrn
Match Type: R21L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-ATAAA TTCAGTATAATTCTAAT  AAAAAGCCCCAACAGGTATGC   ATGCAGCTTGGCAAAAT CATAG-3'
	3'-TATTT AAGTCATATTAAGATTA  TTTTTCGGGGTTGTCCATACG   TACGTCGAACCGTTTTA GTATC-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 001-007380781
Forward Primer: 5'-CACCTCTTCGATCCCTTGCTTTTG-3' Tm: 59.038
Reverse Primer: 5'-GCTGGCAGGCAGATCTCCTT-3' Tm: 59.508
Expected PCR Product Length: 305
Expected CelI Cleavage Product Sizes: 174, 131
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr1:7380926-7380980

Rank: 68	Homology Score: 10	RVD Match Score: 2.849	Left Half-Site RVD Score: 7.669	Right Half-Site RVD Score: 4.296
	Genomic Region: Intergenic	Closest Gene: Mctp2
Match Type: R14L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-AATCA TTCAGTGAATTTGTTGT      GTCACATCTGCCCC      ATGGAGGTGGGAGGGAA GAATA-3'
	3'-TTAGT AAGTCACTTAAACAACA      CAGTGTAGACGGGG      TACCTCCACCCTCCCTT CTTAT-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 001-127719260
Forward Primer: 5'-CCAACATTCTCCCAAGAGATTCTGGAAAC-3' Tm: 60.178
Reverse Primer: 5'-GTTCCAGGTCAACCAGGACTAC-3' Tm: 59.258
Expected PCR Product Length: 368
Expected CelI Cleavage Product Sizes: 135, 233
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr1:127719369-127719416

Rank: 69	Homology Score: 2	RVD Match Score: 2.852	Left Half-Site RVD Score: 6.266	Right Half-Site RVD Score: 5.282
	Genomic Region: Intergenic	Closest Gene: Arid1a
Match Type: R23L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-TCCTA CTCTGTGTTGCTCAGAT TGGTTTTCGAACTCTTAATCTTA  CTGGGTTTGGGTAGGAA TTTCA-3'
	3'-AGGAT GAGACACAACGAGTCTA ACCAAAAGCTTGAGAATTAGAAT  GACCCAAACCCATCCTT AAAGT-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 005-151331548
Forward Primer: 5'-CTGACCCAGGGCAAGTCATTTAC-3' Tm: 59.148
Reverse Primer: 5'-GGACCTCCAACCAGATGAACTAAC-3' Tm: 59.038
Expected PCR Product Length: 368
Expected CelI Cleavage Product Sizes: 173, 195
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr5:151331691-151331747

Rank: 70	Homology Score: 10	RVD Match Score: 2.853	Left Half-Site RVD Score: 5.066	Right Half-Site RVD Score: 6.57
	Genomic Region: Intron	Closest Gene: Ralgapa1
Match Type: L13R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-TCTCC TTCCTTTCCAGCTCCCT      TCTTTCTTTCATT       ACTAGATATGCACTGAA AGCTG-3'
	3'-AGAGG AAGGAAAGGTCGAGGGA      AGAAAGAAAGTAA       TGATCTATACGTGACTT TCGAC-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 006-075907048
Forward Primer: 5'-GGTGTCCTCATTCACCTCTGACAA-3' Tm: 59.038
Reverse Primer: 5'-GTGTTTGGAACATCCTCTGCGAC-3' Tm: 59.148
Expected PCR Product Length: 363
Expected CelI Cleavage Product Sizes: 189, 174
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr6:75907212-75907258

Rank: 71	Homology Score: 10	RVD Match Score: 2.854	Left Half-Site RVD Score: 6.541	Right Half-Site RVD Score: 5.089
	Genomic Region: Intergenic	Closest Gene: Zfand6
Match Type: R15L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-AGGAG TTCAGTCATGTTCAAGG     GCACTTCTTGTGTAA      ATGGGGCTGGACAGGGA GGAGT-3'
	3'-TCCTC AAGTCAGTACAAGTTCC     CGTGAAGAACACATT      TACCCCGACCTGTCCCT CCTCA-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 001-140972716
Forward Primer: 5'-GGGACCACCTCAGCTAAGGA-3' Tm: 59.508
Reverse Primer: 5'-GTGGGGTTGTTGGTTTTCAGCTC-3' Tm: 59.148
Expected PCR Product Length: 368
Expected CelI Cleavage Product Sizes: 173, 195
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr1:140972863-140972911

Rank: 72	Homology Score: 2	RVD Match Score: 2.854	Left Half-Site RVD Score: 4.978	Right Half-Site RVD Score: 6.7
	Genomic Region: Intergenic	Closest Gene: Triml2
Match Type: L22R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-AGAAT ATCCTTCCAAAATCCAT  ATCGTTTGTATTTTGTCTGGGA  AAGAAAATAACACTTAA AACTA-3'
	3'-TCTTA TAGGAAGGTTTTAGGTA  TAGCAAACATAAAACAGACCCT  TTCTTTTATTGTGAATT TTGAT-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 016-053168498
Forward Primer: 5'-CTCTTCAGTAGCTTGGCAACACAG-3' Tm: 59.038
Reverse Primer: 5'-CAAGAAGGAGAGTCATGCCATGAG-3' Tm: 59.038
Expected PCR Product Length: 277
Expected CelI Cleavage Product Sizes: 147, 130
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr16:53168615-53168670

Rank: 73	Homology Score: 5	RVD Match Score: 2.855	Left Half-Site RVD Score: 5.365	Right Half-Site RVD Score: 6.049
	Genomic Region: Exon	Closest Gene: LOC686841
Match Type: R18L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-TGGCC CTCCATGTTGTTCGGGT    GGGTCCTTGTCAAAGTGG    TTGTACCTGGGCAGGGG GGTAA-3'
	3'-ACCGG GAGGTACAACAAGCCCA    CCCAGGAACAGTTTCACC    AACATGGACCCGTCCCC CCATT-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
No Primers
Primer Index: No Primers
Forward Primer: 5'-No Primers-3' Tm: No Primers
Reverse Primer: 5'-No Primers-3' Tm: No Primers
Expected PCR Product Length: No Primers
Expected CelI Cleavage Product Sizes: No Primers, No Primers
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr7:116335856-116335907

Rank: 74	Homology Score: 2	RVD Match Score: 2.857	Left Half-Site RVD Score: 6.005	Right Half-Site RVD Score: 5.426
	Genomic Region: Intergenic	Closest Gene: Cask
Match Type: L12R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-TGGGG ATCCAGCCCATATGCAT       ACAACCACCAAA       CCCAGAACAATATTGTT GATGC-3'
	3'-ACCCC TAGGTCGGGTATACGTA       TGTTGGTGGTTT       GGGTCTTGTTATAACAA CTACG-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 00X-020524409
Forward Primer: 5'-CGAAGTGCAGATGCTTCAGTCC-3' Tm: 59.258
Reverse Primer: 5'-CTCAGTTCAATGGTTGGCTGCTAG-3' Tm: 59.038
Expected PCR Product Length: 277
Expected CelI Cleavage Product Sizes: 145, 132
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chrX:20524529-20524574

Rank: 75	Homology Score: 2	RVD Match Score: 2.857	Left Half-Site RVD Score: 5.804	Right Half-Site RVD Score: 5.661
	Genomic Region: Intergenic	Closest Gene: LOC100363294
Match Type: L19R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-CAGGT GTCCTTCCCATTTCCCT   ACAAGTTGAGTAACATCTG    AGCTTAATAACATTGAA AGATG-3'
	3'-GTCCA CAGGAAGGGTAAAGGGA   TGTTCAACTCATTGTAGAC    TCGAATTATTGTAACTT TCTAC-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 018-027249876
Forward Primer: 5'-CCCCCTATATCCCCTCCTTTTC-3' Tm: 59.258
Reverse Primer: 5'-GCTAGATCCCTGGAACTGGAG-3' Tm: 59.378
Expected PCR Product Length: 312
Expected CelI Cleavage Product Sizes: 163, 149
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr18:27250011-27250063

Rank: 76	Homology Score: 5	RVD Match Score: 2.857	Left Half-Site RVD Score: 6.127	Right Half-Site RVD Score: 5.427
	Genomic Region: Intergenic	Closest Gene: Hpse
Match Type: R12L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-GGAAG GTCAGTATGGTTCAGGC       TCTGCATGTCTG       ATGAAGATGGGCAGAAG GTTCA-3'
	3'-CCTTC CAGTCATACCAAGTCCG       AGACGTACAGAC       TACTTCTACCCGTCTTC CAAGT-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: dom-000592826
Forward Primer: 5'-GCCCTCAATGTTCCTGCTGTAG-3' Tm: 59.258
Reverse Primer: 5'-ACAGGCTATTTTCCATTCCCGCAC-3' Tm: 59.038
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 137, 133
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr14_random:592938-592983

Rank: 77	Homology Score: 5	RVD Match Score: 2.857	Left Half-Site RVD Score: 5.427	Right Half-Site RVD Score: 6.127
	Genomic Region: Intergenic	Closest Gene: Ugt2b
Match Type: L12R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-TGAAC CTTCTGCCCATCTTCAT       CAGACATGCAGA       GCCTGAACCATACTGAC CTTCC-3'
	3'-ACTTG GAAGACGGGTAGAAGTA       GTCTGTACGTCT       CGGACTTGGTATGACTG GAAGG-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 014-022143647
Forward Primer: 5'-AGGCTATTTTCCATTCCCGCACC-3' Tm: 59.148
Reverse Primer: 5'-TGGCCCTCAATGTTCCTGCTG-3' Tm: 59.378
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 135, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr14:22143757-22143802

Rank: 78	Homology Score: 5	RVD Match Score: 2.857	Left Half-Site RVD Score: 5.427	Right Half-Site RVD Score: 6.127
	Genomic Region: Intergenic	Closest Gene: Ugt2b17
Match Type: L12R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-TGAAC CTTCTGCCCATCTTCAT       CAGACATGCAGA       GCCTGAACCATACTGAC CTTCC-3'
	3'-ACTTG GAAGACGGGTAGAAGTA       GTCTGTACGTCT       CGGACTTGGTATGACTG GAAGG-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 014-022284631
Forward Primer: 5'-GCTATCTTCCATTCCCGCACC-3' Tm: 59.378
Reverse Primer: 5'-TTGGCCCTCAATGTTCCTGCTG-3' Tm: 59.258
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 135, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr14:22284741-22284786

Rank: 79	Homology Score: 2	RVD Match Score: 2.859	Left Half-Site RVD Score: 5.126	Right Half-Site RVD Score: 6.541
	Genomic Region: Intron	Closest Gene: Htr2c
Match Type: L14R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-TAAGA ATCCTACCCATTACCAT      TGCCATTGCCATTA      ACCAGTAGACCTTTGAA AAGGT-3'
	3'-ATTCT TAGGATGGGTAATGGTA      ACGGTAACGGTAAT      TGGTCATCTGGAAACTT TTCCA-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 00X-031116233
Forward Primer: 5'-GAGAATGAGCTTTACCAAACCTAAGGTGTG-3' Tm: 60.058
Reverse Primer: 5'-CTGAAGCCTTTGGGATGTATTACTAGGG-3' Tm: 60.298
Expected PCR Product Length: 385
Expected CelI Cleavage Product Sizes: 179, 206
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chrX:31116386-31116433

Rank: 80	Homology Score: 5	RVD Match Score: 2.859	Left Half-Site RVD Score: 5.832	Right Half-Site RVD Score: 5.679
	Genomic Region: Intergenic	Closest Gene: Tubgcp5
Match Type: R14L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-AAAAA GACAGTGTAGTACTGAT      TGAAGAGTTATGTT      ATGGAGATTGGCTTGAA GGTTT-3'
	3'-TTTTT CTGTCACATCATGACTA      ACTTCTCAATACAA      TACCTCTAACCGAACTT CCAAA-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 001-106482030
Forward Primer: 5'-GTGGTGGAACACCCTCTTAGAAGT-3' Tm: 59.038
Reverse Primer: 5'-CTCTATCTGCTGGTGGAATGAAGG-3' Tm: 59.038
Expected PCR Product Length: 271
Expected CelI Cleavage Product Sizes: 138, 133
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr1:106482142-106482189

Rank: 81	Homology Score: 2	RVD Match Score: 2.861	Left Half-Site RVD Score: 6.541	Right Half-Site RVD Score: 5.139
	Genomic Region: Intron	Closest Gene: Antxr1
Match Type: R19L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-AAGTC TGCAGTCAAGTTCTAGT   TTTAATCCAGCTGGTGGCC    ATGTATTTGGGCATGAC TTAAC-3'
	3'-TTCAG ACGTCAGTTCAAGATCA   AAATTAGGTCGACCACCGG    TACATAAACCCGTACTG AATTG-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 004-121347635
Forward Primer: 5'-TTTCAAGGCAGGTCCTGTCTTGG-3' Tm: 59.148
Reverse Primer: 5'-ATGGGGGAATTTCCCCTGGTG-3' Tm: 59.378
Expected PCR Product Length: 271
Expected CelI Cleavage Product Sizes: 130, 141
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr4:121347737-121347789

Rank: 82	Homology Score: 5	RVD Match Score: 2.861	Left Half-Site RVD Score: 4.831	Right Half-Site RVD Score: 6.975
	Genomic Region: Intergenic	Closest Gene: Slc39a11
Match Type: L13R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-CATGG CTCCTATCCAGGTCCAT      GGTTTCTGACGAA       ACTGGCACAACAGAGAA CAGGT-3'
	3'-GTACC GAGGATAGGTCCAGGTA      CCAAAGACTGCTT       TGACCGTGTTGTCTCTT GTCCA-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 010-102159896
Forward Primer: 5'-GGAACCACCCATTTCCTTGGTGT-3' Tm: 59.148
Reverse Primer: 5'-GTGTGTCTGTCTGTCTGTCTGTC-3' Tm: 59.148
Expected PCR Product Length: 291
Expected CelI Cleavage Product Sizes: 146, 145
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr10:102160017-102160063

Rank: 83	Homology Score: 2	RVD Match Score: 2.863	Left Half-Site RVD Score: 5.673	Right Half-Site RVD Score: 5.877
	Genomic Region: Intergenic	Closest Gene: Prep
Match Type: L20R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-CGCGC CTCCTGCACGCCCCCAT   CAGAGTCTGTAACTGGGGTA   ACGTGAGTAAGACTGAA AAGCT-3'
	3'-GCGCG GAGGACGTGCGGGGGTA   GTCTCAGACATTGACCCCAT   TGCACTCATTCTGACTT TTCGA-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 020-049001036
Forward Primer: 5'-GGGGGAGGAGAGTAGTAAATTCG-3' Tm: 59.148
Reverse Primer: 5'-TTCGCGACTTGCCGACGAGA-3' Tm: 59.508
Expected PCR Product Length: 283
Expected CelI Cleavage Product Sizes: 153, 130
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr20:49001160-49001213

Rank: 84	Homology Score: 2	RVD Match Score: 2.864	Left Half-Site RVD Score: 5.503	Right Half-Site RVD Score: 6.096
	Genomic Region: Intergenic	Closest Gene: Trim42
Match Type: L15R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-CTTAG CCCCTCTCCAGCACCAT     GTCTGCATGCAAGTC      ACTAGAAGAACATATAA TGATA-3'
	3'-GAATC GGGGAGAGGTCGTGGTA     CAGACGTACGTTCAG      TGATCTTCTTGTATATT ACTAT-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: dom-000644656
Forward Primer: 5'-GGAGAGAGAAAGGGCGATAGG-3' Tm: 59.378
Reverse Primer: 5'-GCACCTGAGATAAAAGACCCTCTG-3' Tm: 59.038
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 137, 133
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr8_random:644767-644815

Rank: 85	Homology Score: 10	RVD Match Score: 2.865	Left Half-Site RVD Score: 6.046	Right Half-Site RVD Score: 5.448
	Genomic Region: Intergenic	Closest Gene: Fundc1
Match Type: L19R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-AGACC TTCCTAACCCTTTCCAT   TTGCTTAAATTAACAGAGA    AACAGAACAATACTCAA AATTG-3'
	3'-TCTGG AAGGATTGGGAAAGGTA   AACGAATTTAATTGTCTCT    TTGTCTTGTTATGAGTT TTAAC-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 00X-016509931
Forward Primer: 5'-CTACCAATGTCTATGGAGTGACCG-3' Tm: 59.038
Reverse Primer: 5'-GCCTAGAGTATCCCAAATGTGCAC-3' Tm: 59.038
Expected PCR Product Length: 365
Expected CelI Cleavage Product Sizes: 130, 235
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chrX:16510033-16510085

Rank: 86	Homology Score: 5	RVD Match Score: 2.867	Left Half-Site RVD Score: 6.642	Right Half-Site RVD Score: 5.105
	Genomic Region: Intergenic	Closest Gene: Ppp4r2
Match Type: R15L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-TAAAT TTAAATTTTTTTCTGGA     GGGTGATCACAGGGG      TTGGAGTGGGGCAGGCA CAGCA-3'
	3'-ATTTA AATTTAAAAAAAGACCT     CCCACTAGTGTCCCC      AACCTCACCCCGTCCGT GTCGT-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 004-135905706
Forward Primer: 5'-GACAACGACTGCGTTCTATTTGGG-3' Tm: 59.038
Reverse Primer: 5'-TCACGAGCCCTGGTTATTGTTTGG-3' Tm: 59.038
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 134, 136
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr4:135905814-135905862

Rank: 87	Homology Score: 10	RVD Match Score: 2.867	Left Half-Site RVD Score: 7.279	Right Half-Site RVD Score: 4.664
	Genomic Region: Exon	Closest Gene: Lrp1
Match Type: R20L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-GCTGT TTCAATCTTGGGGTGGT   TCCCCCAGTCTGACCAGTAC   ATGGTGCTGGGAAGGGA GAGAT-3'
	3'-CGACA AAGTTAGAACCCCACCA   AGGGGGTCAGACTGGTCATG   TACCACGACCCTTCCCT CTCTA-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 007-067524783
Forward Primer: 5'-TGATCAGAGCCTGCCCCTTC-3' Tm: 59.508
Reverse Primer: 5'-GACAGGGTGCTTCCTCACAATAC-3' Tm: 59.148
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 138, 132
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr7:67524892-67524945

Rank: 88	Homology Score: 2	RVD Match Score: 2.867	Left Half-Site RVD Score: 6.319	Right Half-Site RVD Score: 5.168
	Genomic Region: Intergenic	Closest Gene: RGD1309148
Match Type: L12R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-CCTTT GTTCTGCCCACCTCTGT       TCTCCGTACTCT       ATTAGTATAAAACTGAA GGATT-3'
	3'-GGAAA CAAGACGGGTGGAGACA       AGAGGCATGAGA       TAATCATATTTTGACTT CCTAA-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Warning! Primer pair was generated using suboptimal parameters. 
Primer Index: 005-093127184
Forward Primer: 5'-CCTCCTCTAGTCATTAGAGATAGATCATCATTC-3' Tm: 59.758
Reverse Primer: 5'-CTCCATCCAGATGTAATTCTGAAACTGATGAC-3' Tm: 59.848
Expected PCR Product Length: 423
Expected CelI Cleavage Product Sizes: 171, 252
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr5:93127330-93127375

Rank: 89	Homology Score: 2	RVD Match Score: 2.869	Left Half-Site RVD Score: 4.81	Right Half-Site RVD Score: 7.079
	Genomic Region: Intergenic	Closest Gene: Fchsd2
Match Type: L22R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-GCTGC ACCCTGCCCCACTCCAC  CAATACAGAAAACAAAACAAAT  CCCTGAAACACACTGAG GTTAA-3'
	3'-CGACG TGGGACGGGGTGAGGTG  GTTATGTCTTTTGTTTTGTTTA  GGGACTTTGTGTGACTC CAATT-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 001-158523393
Forward Primer: 5'-TTCAATCCCTACAGCTGGCACG-3' Tm: 59.258
Reverse Primer: 5'-CTTCCCACCAATGGTACAGTGTC-3' Tm: 59.148
Expected PCR Product Length: 276
Expected CelI Cleavage Product Sizes: 131, 145
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr1:158523494-158523549

Rank: 90	Homology Score: 10	RVD Match Score: 2.869	Left Half-Site RVD Score: 5.089	Right Half-Site RVD Score: 6.686
	Genomic Region: Intron	Closest Gene: Tsc2
Match Type: L18R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-TTAGC TACCTGTCCAGCACCAT    GTCTGCCTGGTGCCTCCC    ACCATGACAATAATGGA CTAAA-3'
	3'-AATCG ATGGACAGGTCGTGGTA    CAGACGGACCACGGAGGG    TGGTACTGTTATTACCT GATTT-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 010-013862379
Forward Primer: 5'-CATAGGTGGGGCCTTATCAGAG-3' Tm: 59.258
Reverse Primer: 5'-GGTCCCTAGTCAAGGACATTGTCT-3' Tm: 59.038
Expected PCR Product Length: 273
Expected CelI Cleavage Product Sizes: 131, 142
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr10:13862482-13862533

Rank: 91	Homology Score: 2	RVD Match Score: 2.869	Left Half-Site RVD Score: 5.086	Right Half-Site RVD Score: 6.409
	Genomic Region: Intergenic	Closest Gene: Mis18bp1
Match Type: R14L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-AGAGT CTCATAGTTATTCTGAT      CCTCATCTCATGTG      ATCGAGTTAGAGAGGAA AGTCT-3'
	3'-TCTCA GAGTATCAATAAGACTA      GGAGTAGAGTACAC      TAGCTCAATCTCTCCTT TCAGA-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 006-086619661
Forward Primer: 5'-GGCTTCAGGAAAAGGTTGATTCCC-3' Tm: 59.038
Reverse Primer: 5'-CGTGAAGGAGTCTCGTGTCAAC-3' Tm: 59.258
Expected PCR Product Length: 354
Expected CelI Cleavage Product Sizes: 217, 137
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr6:86619852-86619899

Rank: 92	Homology Score: 2	RVD Match Score: 2.869	Left Half-Site RVD Score: 6.589	Right Half-Site RVD Score: 5.163
	Genomic Region: Intergenic	Closest Gene: Plcl1
Match Type: R19L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-GGAAT TTCAGGGTCATTTTGAT   TTGCATTTCCCTTTGGGCC    TGGGAGGGGGGCAGGAG AGAAG-3'
	3'-CCTTA AAGTCCCAGTAAAACTA   AACGTAAAGGGAAACCCGG    ACCCTCCCCCCGTCCTC TCTTC-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 009-054998586
Forward Primer: 5'-CTCTCTCTCACGGGGTCTAG-3' Tm: 59.508
Reverse Primer: 5'-AGTTCTGAGAGTCTCTCACCTCC-3' Tm: 59.148
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 135, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr9:54998693-54998745

Rank: 93	Homology Score: 2	RVD Match Score: 2.871	Left Half-Site RVD Score: 6.657	Right Half-Site RVD Score: 5.122
	Genomic Region: Intergenic	Closest Gene: Hace1
Match Type: R22L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-CCCTG TAAAATGTGCTTCTGGT  TATTGCAATGTAAGACTTAGGA  GTGGAGGTGGGCTGGGG TTTGC-3'
	3'-GGGAC ATTTTACACGAAGACCA  ATAACGTTACATTCTGAATCCT  CACCTCCACCCGACCCC AAACG-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 020-051519222
Forward Primer: 5'-GTGTTCCTGAACTCTAGTAGAGGC-3' Tm: 59.038
Reverse Primer: 5'-TAGCAGAACTCCTGCCCGTG-3' Tm: 59.508
Expected PCR Product Length: 403
Expected CelI Cleavage Product Sizes: 273, 130
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr20:51519465-51519520

Rank: 94	Homology Score: 5	RVD Match Score: 2.871	Left Half-Site RVD Score: 5.801	Right Half-Site RVD Score: 5.79
	Genomic Region: Intron	Closest Gene: Sema5a
Match Type: R12L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-GCATT ATTAGTTTTCTTCTAGT       GACATATCCATT       ATGGATCAGTGGAGGAA TTGTG-3'
	3'-CGTAA TAATCAAAAGAAGATCA       CTGTATAGGTAA       TACCTAGTCACCTCCTT AACAC-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 002-085130707
Forward Primer: 5'-CATGAGTATAGGACACAGTGCTGC-3' Tm: 59.038
Reverse Primer: 5'-GGGGTCTGGAAACTCAGTAGAGAA-3' Tm: 59.038
Expected PCR Product Length: 334
Expected CelI Cleavage Product Sizes: 140, 194
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr2:85130822-85130867

Rank: 95	Homology Score: 2	RVD Match Score: 2.872	Left Half-Site RVD Score: 6.951	Right Half-Site RVD Score: 4.919
	Genomic Region: Intergenic	Closest Gene: Lmcd1
Match Type: R23L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-AATGT TCTAGTGTCATTCTGTT GCTGTGAAAACACACTGATGGGA  ATGAATTTGGGGAGGAT GATGT-3'
	3'-TTACA AGATCACAGTAAGACAA CGACACTTTTGTGTGACTACCCT  TACTTAAACCCCTCCTA CTACA-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 004-148037768
Forward Primer: 5'-GGCTCCTACCTCCTAAAGATCC-3' Tm: 59.258
Reverse Primer: 5'-CAGAGAGCAAGCCAGTAATCAGC-3' Tm: 59.148
Expected PCR Product Length: 291
Expected CelI Cleavage Product Sizes: 150, 141
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr4:148037888-148037944

Rank: 96	Homology Score: 2	RVD Match Score: 2.872	Left Half-Site RVD Score: 6.327	Right Half-Site RVD Score: 5.197
	Genomic Region: Intergenic	Closest Gene: Kirrel3
Match Type: L19R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-GAATG GTGCTGCCCACTTTCAT   AGTGGGATTTCCTACTTCA    ATCAGAATAACAGAGAC TGTCC-3'
	3'-CTTAC CACGACGGGTGAAAGTA   TCACCCTAAAGGATGAAGT    TAGTCTTATTGTCTCTG ACAGG-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 008-033778331
Forward Primer: 5'-CTAGTCACATCTCATCCCTGCTC-3' Tm: 59.148
Reverse Primer: 5'-GGCCTAGCACTAACTGTGTGATG-3' Tm: 59.148
Expected PCR Product Length: 311
Expected CelI Cleavage Product Sizes: 131, 180
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr8:33778434-33778486

Rank: 97	Homology Score: 5	RVD Match Score: 2.873	Left Half-Site RVD Score: 6.141	Right Half-Site RVD Score: 5.532
	Genomic Region: Intergenic	Closest Gene: Trub1
Match Type: R22L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-TAAAC ATCCGTGTTTATCTGCT  CACAAATCACAGTATCTAGAAA  ATGGAACTGGCCTGGAT GAATG-3'
	3'-ATTTG TAGGCACAAATAGACGA  GTGTTTAGTGTCATAGATCTTT  TACCTTGACCGGACCTA CTTAC-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 001-264057500
Forward Primer: 5'-ACACCCCTTAGAGCTGGCAGA-3' Tm: 59.378
Reverse Primer: 5'-GGGAATATACCTGCACATGTGCAC-3' Tm: 59.038
Expected PCR Product Length: 364
Expected CelI Cleavage Product Sizes: 131, 233
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr1:264057601-264057656

Rank: 98	Homology Score: 2	RVD Match Score: 2.874	Left Half-Site RVD Score: 5.787	Right Half-Site RVD Score: 5.827
	Genomic Region: Intergenic	Closest Gene: Cobl
Match Type: R20L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-TTGGT CTCATCATTGTTCAGGT   CTTCTTTAGGTAGCCATTTG   ATGGGGCTGGAAGGGAT ATAAG-3'
	3'-AACCA GAGTAGTAACAAGTCCA   GAAGAAATCCATCGGTAAAC   TACCCCGACCTTCCCTA TATTC-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 014-093076377
Forward Primer: 5'-GTTCCTCCTCTCTCATCTCAGC-3' Tm: 59.258
Reverse Primer: 5'-CCAAGGAGTCATATAGCTGGGTC-3' Tm: 59.148
Expected PCR Product Length: 287
Expected CelI Cleavage Product Sizes: 132, 155
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr14:93076480-93076533

Rank: 99	Homology Score: 8	RVD Match Score: 2.876	Left Half-Site RVD Score: 6.164	Right Half-Site RVD Score: 5.532
	Genomic Region: Intergenic	Closest Gene: Amigo2
Match Type: R18L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-GGTTC TTCAGTGATGTACTGCA    TCAGGATGTGTAAGACAG    ATGAACCTGGGCTGGAG AGATG-3'
	3'-CCAAG AAGTCACTACATGACGT    AGTCCTACACATTCTGTC    TACTTGGACCCGACCTC TCTAC-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 007-135956125
Forward Primer: 5'-CACTCCTACGTAGGTATCTTGGG-3' Tm: 59.148
Reverse Primer: 5'-CTCACCAGAAGAGGGCATCAAATC-3' Tm: 59.038
Expected PCR Product Length: 280
Expected CelI Cleavage Product Sizes: 137, 143
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr7:135956234-135956285

Rank: 100	Homology Score: 2	RVD Match Score: 2.877	Left Half-Site RVD Score: 7.16	Right Half-Site RVD Score: 4.81
	Genomic Region: Intron	Closest Gene: Kdm5b
Match Type: R15L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-TCTCC TTCAGTAAGGGTCTGTT     CCTCATGGATTGTGG      GTGGAGTGGGGCGGGAT GGGGG-3'
	3'-AGAGG AAGTCATTCCCAGACAA     GGAGTACCTAACACC      CACCTCACCCCGCCCTA CCCCC-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 013-047528534
Forward Primer: 5'-ATCCCCTGTAGAGATCTTGGACTG-3' Tm: 59.038
Reverse Primer: 5'-GCTCCTGTCTAGCTTATCATCCTG-3' Tm: 59.038
Expected PCR Product Length: 323
Expected CelI Cleavage Product Sizes: 130, 193
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr13:47528638-47528686

Plate format Oligo Order Form
Well
Name
Sequence
5' mods
Internal
Notes
Primer Plate #1
A1
006-138443970F
GGAATCGGCACATGCAGATCTTTG
Tesson_IgM_Ranking-RVDs	Primer pair generated using optimal parameters. 
A2
006-138443970R
TCTGGTCCTCAGAGAGTCAGTC
Tesson_IgM_Ranking-RVDs	Primer pair generated using optimal parameters. 
B1
001-256112389F
CCTGGATAAGTCAGTAAAGGGTGG
Tesson_IgM_Ranking-RVDs	Primer pair generated using optimal parameters. 
B2
001-256112389R
CCTTGGTGGGACCTGATACAGT
Tesson_IgM_Ranking-RVDs	Primer pair generated using optimal parameters. 
C1
001-120607423F
AGGTCAGTGTCCCTGGCCTT
Tesson_IgM_Ranking-RVDs	Primer pair generated using optimal parameters. 
C2
001-120607423R
GGCTGGCCAATTCACAATCCTC
Tesson_IgM_Ranking-RVDs	Primer pair generated using optimal parameters. 
D1
003-149194978F
CTTGTTAGGTGAAGCAGGATGGC
Tesson_IgM_Ranking-RVDs	Primer pair generated using optimal parameters. 
D2
003-149194978R
TCACACCGGTGCTGAAGGAAC
Tesson_IgM_Ranking-RVDs	Primer pair generated using optimal parameters. 
E1
006-046986724F
CTTCTGGCTTAGTTTCCGTCTGTC
Tesson_IgM_Ranking-RVDs	Primer pair generated using optimal parameters. 
E2
006-046986724R
CTCAGCTTGCCCAAGGGTTATAG
Tesson_IgM_Ranking-RVDs	Primer pair generated using optimal parameters. 
F1
014-018268832F
TTTCATGGCCTTCTGCAGCCATGT
Tesson_IgM_Ranking-RVDs	Primer pair generated using optimal parameters. 
F2
014-018268832R
GCAGCAAACACACTACAAATGGGG
Tesson_IgM_Ranking-RVDs	Primer pair generated using optimal parameters. 
G1
001-252428669F
GTTGTGTTTCAGGACAGCACTACC
Tesson_IgM_Ranking-RVDs	Primer pair generated using optimal parameters. 
G2
001-252428669R
GTTGGAGAGGCCACATTCTTAACC
Tesson_IgM_Ranking-RVDs	Primer pair generated using optimal parameters. 
H1
007-139879252F
AAAGGCTCATTAGACACGCTCAGG
Tesson_IgM_Ranking-RVDs	Primer pair generated using optimal parameters. 
H2
007-139879252R
CACGTCTCTGCAGACCCCAT
Tesson_IgM_Ranking-RVDs	Primer pair generated using optimal parameters. 
A3
003-151465311F
CACACACACACACACACACACAC
Tesson_IgM_Ranking-RVDs	Primer pair generated using optimal parameters. 
A4
003-151465311R
CACCAGTGACAACAGCACTTGAG
Tesson_IgM_Ranking-RVDs	Primer pair generated using optimal parameters. 
B3
011-011914436F
GACACAGTACTCAGAGGATCCAAG
Tesson_IgM_Ranking-RVDs	Primer pair generated using optimal parameters. 
B4
011-011914436R
GCTTGGCTTCTAGGACCAGAC
Tesson_IgM_Ranking-RVDs	Primer pair generated using optimal parameters. 
C3
018-045099807F
GGCTTAGGGTAACATCAGTACCAG
Tesson_IgM_Ranking-RVDs	Primer pair generated using optimal parameters. 
C4
018-045099807R
AAAAGCAGGCCGGTAGGCAG
Tesson_IgM_Ranking-RVDs	Primer pair generated using optimal parameters. 
D3
00X-027254372F
CCATTATCGCTAGCCTAGCTTACG
Tesson_IgM_Ranking-RVDs	Primer pair generated using optimal parameters. 
D4
00X-027254372R
GACTGGGAATAAGTGGTGTGGTAC
Tesson_IgM_Ranking-RVDs	Primer pair generated using optimal parameters. 
E3
dom-000581844F
ATGTGCCTGCAAGGTGGCTG
Tesson_IgM_Ranking-RVDs	Primer pair generated using optimal parameters. 
E4
dom-000581844R
GCTCCAGCCCATATTTGCAGTTC
Tesson_IgM_Ranking-RVDs	Primer pair generated using optimal parameters. 
F3
009-107302997F
GGCAAACTGACCAGGTCATGTG
Tesson_IgM_Ranking-RVDs	Primer pair generated using optimal parameters. 
F4
009-107302997R
GAGCTCCTGCTACCAATTATGTGC
Tesson_IgM_Ranking-RVDs	Primer pair generated using optimal parameters. 
G3
001-189623849F
TGCATTCCCACCCCCATACTG
Tesson_IgM_Ranking-RVDs	Primer pair generated using optimal parameters. 
G4
001-189623849R
CTGAGCACATTCAGGCATCTGG
Tesson_IgM_Ranking-RVDs	Primer pair generated using optimal parameters. 
H3
007-062964516F
TCAAGGCGCAGTCAACCTCC
Tesson_IgM_Ranking-RVDs	Primer pair generated using optimal parameters. 
H4
007-062964516R
CTGTCAAGGTTGAACAAGGGACTG
Tesson_IgM_Ranking-RVDs	Primer pair generated using optimal parameters. 
A5
009-007190270F
AGTTGGGCTGGTAGAAATTGCTGG
Tesson_IgM_Ranking-RVDs	Primer pair generated using optimal parameters. 
A6
009-007190270R
CTGAGTGAAACAAACGGCTCCGA
Tesson_IgM_Ranking-RVDs	Primer pair generated using optimal parameters. 
B5
005-036123844F
GGCTGTAGACACTCTTCAGCTTG
Tesson_IgM_Ranking-RVDs	Primer pair generated using optimal parameters. 
B6
005-036123844R
GTGACCTCTCTGCAGACAGC
Tesson_IgM_Ranking-RVDs	Primer pair generated using optimal parameters. 
C5
006-090557385F
GTCCCCACAGGCATATGATCG
Tesson_IgM_Ranking-RVDs	Primer pair generated using optimal parameters. 
C6
006-090557385R
GTGCTTCGGGCAGTAGAGCT
Tesson_IgM_Ranking-RVDs	Primer pair generated using optimal parameters. 
D5
017-089324125F
GGATTTGGGAGAGGAAGGATGAG
Tesson_IgM_Ranking-RVDs	Primer pair generated using optimal parameters. 
D6
017-089324125R
CATAGTGACACACCCTGAAGTTGC
Tesson_IgM_Ranking-RVDs	Primer pair generated using optimal parameters. 
E5
014-008802083F
TGCCAGGCTCCTGTCTGCAT
Tesson_IgM_Ranking-RVDs	Primer pair generated using optimal parameters. 
E6
014-008802083R
GCTACCAGGACTCAATGGGG
Tesson_IgM_Ranking-RVDs	Primer pair generated using optimal parameters. 
F5
007-142568536F
CCCAAGTTGCTTTTGGTCACGATG
Tesson_IgM_Ranking-RVDs	Primer pair generated using optimal parameters. 
F6
007-142568536R
ACCCGAGGACTTGAGCCAGA
Tesson_IgM_Ranking-RVDs	Primer pair generated using optimal parameters. 
G5
0Un-024874250F
GCCTTCAATTCAGTGAGGAATGGG
Tesson_IgM_Ranking-RVDs	Primer pair generated using optimal parameters. 
G6
0Un-024874250R
TACTCAAGTGTGTGCCATGCACC
Tesson_IgM_Ranking-RVDs	Primer pair generated using optimal parameters. 
H5
003-107514838F
GTTGGCTTAAGGCTGCCAACTTC
Tesson_IgM_Ranking-RVDs	Primer pair generated using optimal parameters. 
H6
003-107514838R
GTGTGTTGCCTCCTTTTCTACACG
Tesson_IgM_Ranking-RVDs	Primer pair generated using optimal parameters. 
A7
020-043706993F
GCAGAAATGGATAGTTTGTGGGGG
Tesson_IgM_Ranking-RVDs	Primer pair generated using optimal parameters. Warning! The sequence of this region of the genome is poorly mapped. 
A8
020-043706993R
CCGCCAAGCCCTTTTTAAGTACTG
Tesson_IgM_Ranking-RVDs	Primer pair generated using optimal parameters. Warning! The sequence of this region of the genome is poorly mapped. 
B7
007-131749648F
GGCTTCCTACCCAGCTTTATTTCC
Tesson_IgM_Ranking-RVDs	Primer pair generated using optimal parameters. 
B8
007-131749648R
GGACTCTGAGTTGCTGAGCC
Tesson_IgM_Ranking-RVDs	Primer pair generated using optimal parameters. 
C7
016-044835388F
GTCCCATAACTGGGGCTAGG
Tesson_IgM_Ranking-RVDs	Primer pair generated using optimal parameters. 
C8
016-044835388R
CCCTCTTAGGTCCATTCCACTAG
Tesson_IgM_Ranking-RVDs	Primer pair generated using optimal parameters. 
D7
010-070982895F
CAGGGTGTGTCTTTCCCAAGATAC
Tesson_IgM_Ranking-RVDs	Primer pair generated using optimal parameters. 
D8
010-070982895R
GGGTCTCAGGAAACGTTGTGTTG
Tesson_IgM_Ranking-RVDs	Primer pair generated using optimal parameters. 
E7
001-245506315F
AGGTCAGAGGTGAGGCAGCA
Tesson_IgM_Ranking-RVDs	Primer pair generated using optimal parameters. 
E8
001-245506315R
TCACTGCATGTCCATGCGCAAC
Tesson_IgM_Ranking-RVDs	Primer pair generated using optimal parameters. 
F7
004-126653383F
CCATGTGACCTTTGGACTACATGC
Tesson_IgM_Ranking-RVDs	Primer pair generated using optimal parameters. 
F8
004-126653383R
GAGAGCCACTGACTACCATAACAC
Tesson_IgM_Ranking-RVDs	Primer pair generated using optimal parameters. 
G7
00X-074124866F
AATGCCCTACAGTGGGGAGAG
Tesson_IgM_Ranking-RVDs	Primer pair generated using optimal parameters. 
G8
00X-074124866R
CAAAGGACAGTCATGATAGGTCCC
Tesson_IgM_Ranking-RVDs	Primer pair generated using optimal parameters. 
H7
001-082505606F
TAAGGTGGGGGTGTGGCTGA
Tesson_IgM_Ranking-RVDs	Primer pair generated using optimal parameters. 
H8
001-082505606R
CCAACGCCAACTACAACCTGC
Tesson_IgM_Ranking-RVDs	Primer pair generated using optimal parameters. 
A9
014-082098705F
AGCCAAGGAAGAAACCTGTGCAGC
Tesson_IgM_Ranking-RVDs	Primer pair generated using optimal parameters. 
A10
014-082098705R
CTGTGGATGGCTAGAGTGTACTCA
Tesson_IgM_Ranking-RVDs	Primer pair generated using optimal parameters. 
B9
019-057896612F
CCCAGTGCTGGGGTTACAAACA
Tesson_IgM_Ranking-RVDs	Primer pair generated using optimal parameters. 
B10
019-057896612R
TCTTAGCCAGGAATGAGGCTGC
Tesson_IgM_Ranking-RVDs	Primer pair generated using optimal parameters. 
C9
012-043552977F
CACCCACTTCATGTTGGAAACGC
Tesson_IgM_Ranking-RVDs	Primer pair generated using optimal parameters. 
C10
012-043552977R
TTCCAAACCCTCTGCCCTGC
Tesson_IgM_Ranking-RVDs	Primer pair generated using optimal parameters. 
D9
010-014405848F
CCTGGTGTTCAGCCTTGGGT
Tesson_IgM_Ranking-RVDs	Primer pair generated using optimal parameters. 
D10
010-014405848R
TGCTGTCACCTGACCTCAGAGA
Tesson_IgM_Ranking-RVDs	Primer pair generated using optimal parameters. 
E9
005-123636672F
CTCTGAGTGACACGAATTAGTGGG
Tesson_IgM_Ranking-RVDs	Primer pair generated using optimal parameters. 
E10
005-123636672R
GGAGTGTGTCACTAAGGCTGG
Tesson_IgM_Ranking-RVDs	Primer pair generated using optimal parameters. 
F9
003-128243778F
CAACCACACTGCAGACCAGG
Tesson_IgM_Ranking-RVDs	Primer pair generated using optimal parameters. 
F10
003-128243778R
GCCATGGATCTGATATTGGTGGCT
Tesson_IgM_Ranking-RVDs	Primer pair generated using optimal parameters. 
G9
003-096182235F
CTGTTCATCCTCCCTGTGGAAG
Tesson_IgM_Ranking-RVDs	Primer pair generated using optimal parameters. 
G10
003-096182235R
TTGCGGCATGCTTGAGTTTGCCC
Tesson_IgM_Ranking-RVDs	Primer pair generated using optimal parameters. 
H9
dom-004607360F
GGCCAACTCTTGAGAGAACAAGTG
Tesson_IgM_Ranking-RVDs	Primer pair generated using optimal parameters. 
H10
dom-004607360R
CTATCTTGTATTCGCAGCGCCTC
Tesson_IgM_Ranking-RVDs	Primer pair generated using optimal parameters. 
A11
003-100952790F
GCCATGAAGAAGACATGCAGGAG
Tesson_IgM_Ranking-RVDs	Primer pair generated using optimal parameters. 
A12
003-100952790R
GGATCACTTGAAATGTGGAGCTTCCTAC
Tesson_IgM_Ranking-RVDs	Primer pair generated using optimal parameters. 
B11
007-022951295F
TGAGACCTCCTTTTCCTTCTCCTG
Tesson_IgM_Ranking-RVDs	Primer pair generated using optimal parameters. 
B12
007-022951295R
ATATGCCCCTGCAAGGAGCC
Tesson_IgM_Ranking-RVDs	Primer pair generated using optimal parameters. 
C11
001-138291107F
TATCTATGTGGCAGGCTGGGTAC
Tesson_IgM_Ranking-RVDs	Primer pair generated using optimal parameters. 
C12
001-138291107R
GTGCAGTGTCATACCCGGGTT
Tesson_IgM_Ranking-RVDs	Primer pair generated using optimal parameters. 
D11
004-025342811F
CCAGTACCAGGTATAACTTCCCTC
Tesson_IgM_Ranking-RVDs	Primer pair generated using optimal parameters. 
D12
004-025342811R
GGACCCAGAGACACTGAGCT
Tesson_IgM_Ranking-RVDs	Primer pair generated using optimal parameters. 
E11
017-057702366F
CGGCACATAATCCTGCCTCTC
Tesson_IgM_Ranking-RVDs	Primer pair generated using optimal parameters. 
E12
017-057702366R
GTCTAGTGATCCCTCCCTACTTTG
Tesson_IgM_Ranking-RVDs	Primer pair generated using optimal parameters. 
F11
012-027291771F
TCCTAAGGGTGGCCTTTTGACCTC
Tesson_IgM_Ranking-RVDs	Primer pair generated using optimal parameters. 
F12
012-027291771R
CAGCCTCAGCTCACAGAGATC
Tesson_IgM_Ranking-RVDs	Primer pair generated using optimal parameters. 
G11
010-005637007F
ACGAATGCACAGAAACAGCTGTGG
Tesson_IgM_Ranking-RVDs	Primer pair generated using optimal parameters. 
G12
010-005637007R
GCTAAGAAGCAGACGCTGTGCA
Tesson_IgM_Ranking-RVDs	Primer pair generated using optimal parameters. 
H11
020-016460972F
GTCAAGGACGAAGTCATCTGGAG
Tesson_IgM_Ranking-RVDs	Primer pair generated using optimal parameters. 
H12
020-016460972R
GACAAGCTGACTGCTGCTAGAG
Tesson_IgM_Ranking-RVDs	Primer pair generated using optimal parameters. 
Primer Plate #2
A1
012-033556886F
TCTGTCTGCAAGCTCCAGGG
Tesson_IgM_Ranking-RVDs	Primer pair generated using optimal parameters. 
A2
012-033556886R
AACTCTGGCCTCCTTGGTCC
Tesson_IgM_Ranking-RVDs	Primer pair generated using optimal parameters. 
B1
020-014621051F
GCCTGTGGTTGGTACATGTACCT
Tesson_IgM_Ranking-RVDs	Primer pair generated using optimal parameters. 
B2
020-014621051R
GAGCCGTGATTCTCCATGGATTC
Tesson_IgM_Ranking-RVDs	Primer pair generated using optimal parameters. 
C1
009-006766208F
GCTCTGGAGAAAGGTTGCCATG
Tesson_IgM_Ranking-RVDs	Primer pair generated using optimal parameters. 
C2
009-006766208R
CTGGAGAAGCTGCCAGAGGA
Tesson_IgM_Ranking-RVDs	Primer pair generated using optimal parameters. 
D1
007-038298476F
AAAAGCATGAGCTCCTCAGGCTGG
Tesson_IgM_Ranking-RVDs	Primer pair generated using optimal parameters. 
D2
007-038298476R
GAAGAGGAAACTGTGGTCAGGTTG
Tesson_IgM_Ranking-RVDs	Primer pair generated using optimal parameters. 
E1
002-204001007F
AATTTATGGGCTCTGTGCCGTGC
Tesson_IgM_Ranking-RVDs	Primer pair generated using optimal parameters. 
E2
002-204001007R
CCCAGTGCTAAGTTGAGTGTGGC
Tesson_IgM_Ranking-RVDs	Primer pair generated using optimal parameters. 
F1
016-077159880F
CTGTGGAGTTTGTGCAGGAATGCT
Tesson_IgM_Ranking-RVDs	Primer pair generated using optimal parameters. 
F2
016-077159880R
GGGGCAGTGTGTGATCAAAAGG
Tesson_IgM_Ranking-RVDs	Primer pair generated using optimal parameters. 
G1
002-057295241F
GGCACTCTTGGGATGAGTGTAG
Tesson_IgM_Ranking-RVDs	Primer pair generated using optimal parameters. 
G2
002-057295241R
GCTCTACTCTCATCTGCTGATTAATCTCTG
Tesson_IgM_Ranking-RVDs	Primer pair generated using optimal parameters. 
H1
003-157836620F
GGCAAAGCTGCCTGTCACCA
Tesson_IgM_Ranking-RVDs	Primer pair generated using optimal parameters. 
H2
003-157836620R
GCTTCAGGCACAGCTGGATC
Tesson_IgM_Ranking-RVDs	Primer pair generated using optimal parameters. 
A3
010-017513398F
CTCATGCTTGTGCAGCAAGCACT
Tesson_IgM_Ranking-RVDs	Primer pair generated using optimal parameters. 
A4
010-017513398R
TTGATGGGGCAGGGCATCAG
Tesson_IgM_Ranking-RVDs	Primer pair generated using optimal parameters. 
B3
002-159432579F
GCTAAATCCCCAACCCTGCAG
Tesson_IgM_Ranking-RVDs	Primer pair generated using optimal parameters. 
B4
002-159432579R
GTGTTTACTTGCTCAAACACCCGG
Tesson_IgM_Ranking-RVDs	Primer pair generated using optimal parameters. 
C3
015-023818731F
CACTAGGCCATGTGTAGTAGCTG
Tesson_IgM_Ranking-RVDs	Primer pair generated using optimal parameters. 
C4
015-023818731R
GTCCACCCCATATAAAGTGTACGC
Tesson_IgM_Ranking-RVDs	Primer pair generated using optimal parameters. 
D3
003-106797759F
GCTTCTTGTTCTTCATTCCCCTGG
Tesson_IgM_Ranking-RVDs	Primer pair generated using optimal parameters. 
D4
003-106797759R
TCACACTTCTCAGGCTGCTTTGG
Tesson_IgM_Ranking-RVDs	Primer pair generated using optimal parameters. 
E3
005-067767253F
CCCAATAATTCTTACCCTCTTGGGGGA
Tesson_IgM_Ranking-RVDs	Primer pair generated using optimal parameters. 
E4
005-067767253R
GTGGCCCTTAACTGATCTGGCT
Tesson_IgM_Ranking-RVDs	Primer pair generated using optimal parameters. 
F3
008-084548726F
TTCTATTATTTCATGAAAATGAAGGCATGGCATGGTG
Tesson_IgM_Ranking-RVDs	Warning! Primer pair was generated using suboptimal parameters. Warning! The sequence of this region of the genome is poorly mapped. 
F4
008-084548726R
CCTTATGGGTGTTCCCCTCC
Tesson_IgM_Ranking-RVDs	Warning! Primer pair was generated using suboptimal parameters. Warning! The sequence of this region of the genome is poorly mapped. 
G3
016-060358235F
CTCTGCATCAGTCCTGCCTTC
Tesson_IgM_Ranking-RVDs	Primer pair generated using optimal parameters. 
G4
016-060358235R
GGGCTACAGAGTAAGACTGTGTCA
Tesson_IgM_Ranking-RVDs	Primer pair generated using optimal parameters. 
H3
002-117980578F
CAACACACACACACAGGTTTCCTC
Tesson_IgM_Ranking-RVDs	Primer pair generated using optimal parameters. 
H4
002-117980578R
CTCTACTGTGTTTGGAGGAGGATG
Tesson_IgM_Ranking-RVDs	Primer pair generated using optimal parameters. 
A5
018-071340665F
CCCCCATCCCCTTTTACAGTTC
Tesson_IgM_Ranking-RVDs	Primer pair generated using optimal parameters. 
A6
018-071340665R
GTAGAGGTCCCTCCTTGAGATTC
Tesson_IgM_Ranking-RVDs	Primer pair generated using optimal parameters. 
B5
013-038906135F
GCCCCACAGCTATCTCTTTTTGGA
Tesson_IgM_Ranking-RVDs	Primer pair generated using optimal parameters. 
B6
013-038906135R
CTGGAGACAGCTTTCAAATCACCC
Tesson_IgM_Ranking-RVDs	Primer pair generated using optimal parameters. 
C5
001-007380781F
CACCTCTTCGATCCCTTGCTTTTG
Tesson_IgM_Ranking-RVDs	Primer pair generated using optimal parameters. 
C6
001-007380781R
GCTGGCAGGCAGATCTCCTT
Tesson_IgM_Ranking-RVDs	Primer pair generated using optimal parameters. 
D5
001-127719260F
CCAACATTCTCCCAAGAGATTCTGGAAAC
Tesson_IgM_Ranking-RVDs	Primer pair generated using optimal parameters. 
D6
001-127719260R
GTTCCAGGTCAACCAGGACTAC
Tesson_IgM_Ranking-RVDs	Primer pair generated using optimal parameters. 
E5
005-151331548F
CTGACCCAGGGCAAGTCATTTAC
Tesson_IgM_Ranking-RVDs	Primer pair generated using optimal parameters. 
E6
005-151331548R
GGACCTCCAACCAGATGAACTAAC
Tesson_IgM_Ranking-RVDs	Primer pair generated using optimal parameters. 
F5
006-075907048F
GGTGTCCTCATTCACCTCTGACAA
Tesson_IgM_Ranking-RVDs	Primer pair generated using optimal parameters. 
F6
006-075907048R
GTGTTTGGAACATCCTCTGCGAC
Tesson_IgM_Ranking-RVDs	Primer pair generated using optimal parameters. 
G5
001-140972716F
GGGACCACCTCAGCTAAGGA
Tesson_IgM_Ranking-RVDs	Primer pair generated using optimal parameters. 
G6
001-140972716R
GTGGGGTTGTTGGTTTTCAGCTC
Tesson_IgM_Ranking-RVDs	Primer pair generated using optimal parameters. 
H5
016-053168498F
CTCTTCAGTAGCTTGGCAACACAG
Tesson_IgM_Ranking-RVDs	Primer pair generated using optimal parameters. 
H6
016-053168498R
CAAGAAGGAGAGTCATGCCATGAG
Tesson_IgM_Ranking-RVDs	Primer pair generated using optimal parameters. 
A7
No PrimersF
No Primers
Tesson_IgM_Ranking-RVDs	No Primers
A8
No PrimersR
No Primers
Tesson_IgM_Ranking-RVDs	No Primers
B7
00X-020524409F
CGAAGTGCAGATGCTTCAGTCC
Tesson_IgM_Ranking-RVDs	Primer pair generated using optimal parameters. 
B8
00X-020524409R
CTCAGTTCAATGGTTGGCTGCTAG
Tesson_IgM_Ranking-RVDs	Primer pair generated using optimal parameters. 
C7
018-027249876F
CCCCCTATATCCCCTCCTTTTC
Tesson_IgM_Ranking-RVDs	Primer pair generated using optimal parameters. 
C8
018-027249876R
GCTAGATCCCTGGAACTGGAG
Tesson_IgM_Ranking-RVDs	Primer pair generated using optimal parameters. 
D7
dom-000592826F
GCCCTCAATGTTCCTGCTGTAG
Tesson_IgM_Ranking-RVDs	Primer pair generated using optimal parameters. 
D8
dom-000592826R
ACAGGCTATTTTCCATTCCCGCAC
Tesson_IgM_Ranking-RVDs	Primer pair generated using optimal parameters. 
E7
014-022143647F
AGGCTATTTTCCATTCCCGCACC
Tesson_IgM_Ranking-RVDs	Primer pair generated using optimal parameters. 
E8
014-022143647R
TGGCCCTCAATGTTCCTGCTG
Tesson_IgM_Ranking-RVDs	Primer pair generated using optimal parameters. 
F7
014-022284631F
GCTATCTTCCATTCCCGCACC
Tesson_IgM_Ranking-RVDs	Primer pair generated using optimal parameters. 
F8
014-022284631R
TTGGCCCTCAATGTTCCTGCTG
Tesson_IgM_Ranking-RVDs	Primer pair generated using optimal parameters. 
G7
00X-031116233F
GAGAATGAGCTTTACCAAACCTAAGGTGTG
Tesson_IgM_Ranking-RVDs	Primer pair generated using optimal parameters. 
G8
00X-031116233R
CTGAAGCCTTTGGGATGTATTACTAGGG
Tesson_IgM_Ranking-RVDs	Primer pair generated using optimal parameters. 
H7
001-106482030F
GTGGTGGAACACCCTCTTAGAAGT
Tesson_IgM_Ranking-RVDs	Primer pair generated using optimal parameters. 
H8
001-106482030R
CTCTATCTGCTGGTGGAATGAAGG
Tesson_IgM_Ranking-RVDs	Primer pair generated using optimal parameters. 
A9
004-121347635F
TTTCAAGGCAGGTCCTGTCTTGG
Tesson_IgM_Ranking-RVDs	Primer pair generated using optimal parameters. 
A10
004-121347635R
ATGGGGGAATTTCCCCTGGTG
Tesson_IgM_Ranking-RVDs	Primer pair generated using optimal parameters. 
B9
010-102159896F
GGAACCACCCATTTCCTTGGTGT
Tesson_IgM_Ranking-RVDs	Primer pair generated using optimal parameters. 
B10
010-102159896R
GTGTGTCTGTCTGTCTGTCTGTC
Tesson_IgM_Ranking-RVDs	Primer pair generated using optimal parameters. 
C9
020-049001036F
GGGGGAGGAGAGTAGTAAATTCG
Tesson_IgM_Ranking-RVDs	Primer pair generated using optimal parameters. 
C10
020-049001036R
TTCGCGACTTGCCGACGAGA
Tesson_IgM_Ranking-RVDs	Primer pair generated using optimal parameters. 
D9
dom-000644656F
GGAGAGAGAAAGGGCGATAGG
Tesson_IgM_Ranking-RVDs	Primer pair generated using optimal parameters. 
D10
dom-000644656R
GCACCTGAGATAAAAGACCCTCTG
Tesson_IgM_Ranking-RVDs	Primer pair generated using optimal parameters. 
E9
00X-016509931F
CTACCAATGTCTATGGAGTGACCG
Tesson_IgM_Ranking-RVDs	Primer pair generated using optimal parameters. 
E10
00X-016509931R
GCCTAGAGTATCCCAAATGTGCAC
Tesson_IgM_Ranking-RVDs	Primer pair generated using optimal parameters. 
F9
004-135905706F
GACAACGACTGCGTTCTATTTGGG
Tesson_IgM_Ranking-RVDs	Primer pair generated using optimal parameters. 
F10
004-135905706R
TCACGAGCCCTGGTTATTGTTTGG
Tesson_IgM_Ranking-RVDs	Primer pair generated using optimal parameters. 
G9
007-067524783F
TGATCAGAGCCTGCCCCTTC
Tesson_IgM_Ranking-RVDs	Primer pair generated using optimal parameters. 
G10
007-067524783R
GACAGGGTGCTTCCTCACAATAC
Tesson_IgM_Ranking-RVDs	Primer pair generated using optimal parameters. 
H9
005-093127184F
CCTCCTCTAGTCATTAGAGATAGATCATCATTC
Tesson_IgM_Ranking-RVDs	Warning! Primer pair was generated using suboptimal parameters. 
H10
005-093127184R
CTCCATCCAGATGTAATTCTGAAACTGATGAC
Tesson_IgM_Ranking-RVDs	Warning! Primer pair was generated using suboptimal parameters. 
A11
001-158523393F
TTCAATCCCTACAGCTGGCACG
Tesson_IgM_Ranking-RVDs	Primer pair generated using optimal parameters. 
A12
001-158523393R
CTTCCCACCAATGGTACAGTGTC
Tesson_IgM_Ranking-RVDs	Primer pair generated using optimal parameters. 
B11
010-013862379F
CATAGGTGGGGCCTTATCAGAG
Tesson_IgM_Ranking-RVDs	Primer pair generated using optimal parameters. 
B12
010-013862379R
GGTCCCTAGTCAAGGACATTGTCT
Tesson_IgM_Ranking-RVDs	Primer pair generated using optimal parameters. 
C11
006-086619661F
GGCTTCAGGAAAAGGTTGATTCCC
Tesson_IgM_Ranking-RVDs	Primer pair generated using optimal parameters. 
C12
006-086619661R
CGTGAAGGAGTCTCGTGTCAAC
Tesson_IgM_Ranking-RVDs	Primer pair generated using optimal parameters. 
D11
009-054998586F
CTCTCTCTCACGGGGTCTAG
Tesson_IgM_Ranking-RVDs	Primer pair generated using optimal parameters. 
D12
009-054998586R
AGTTCTGAGAGTCTCTCACCTCC
Tesson_IgM_Ranking-RVDs	Primer pair generated using optimal parameters. 
E11
020-051519222F
GTGTTCCTGAACTCTAGTAGAGGC
Tesson_IgM_Ranking-RVDs	Primer pair generated using optimal parameters. 
E12
020-051519222R
TAGCAGAACTCCTGCCCGTG
Tesson_IgM_Ranking-RVDs	Primer pair generated using optimal parameters. 
F11
002-085130707F
CATGAGTATAGGACACAGTGCTGC
Tesson_IgM_Ranking-RVDs	Primer pair generated using optimal parameters. 
F12
002-085130707R
GGGGTCTGGAAACTCAGTAGAGAA
Tesson_IgM_Ranking-RVDs	Primer pair generated using optimal parameters. 
G11
004-148037768F
GGCTCCTACCTCCTAAAGATCC
Tesson_IgM_Ranking-RVDs	Primer pair generated using optimal parameters. 
G12
004-148037768R
CAGAGAGCAAGCCAGTAATCAGC
Tesson_IgM_Ranking-RVDs	Primer pair generated using optimal parameters. 
H11
008-033778331F
CTAGTCACATCTCATCCCTGCTC
Tesson_IgM_Ranking-RVDs	Primer pair generated using optimal parameters. 
H12
008-033778331R
GGCCTAGCACTAACTGTGTGATG
Tesson_IgM_Ranking-RVDs	Primer pair generated using optimal parameters. 
Primer Plate #3
A1
001-264057500F
ACACCCCTTAGAGCTGGCAGA
Tesson_IgM_Ranking-RVDs	Primer pair generated using optimal parameters. 
A2
001-264057500R
GGGAATATACCTGCACATGTGCAC
Tesson_IgM_Ranking-RVDs	Primer pair generated using optimal parameters. 
B1
014-093076377F
GTTCCTCCTCTCTCATCTCAGC
Tesson_IgM_Ranking-RVDs	Primer pair generated using optimal parameters. 
B2
014-093076377R
CCAAGGAGTCATATAGCTGGGTC
Tesson_IgM_Ranking-RVDs	Primer pair generated using optimal parameters. 
C1
007-135956125F
CACTCCTACGTAGGTATCTTGGG
Tesson_IgM_Ranking-RVDs	Primer pair generated using optimal parameters. 
C2
007-135956125R
CTCACCAGAAGAGGGCATCAAATC
Tesson_IgM_Ranking-RVDs	Primer pair generated using optimal parameters. 
D1
013-047528534F
ATCCCCTGTAGAGATCTTGGACTG
Tesson_IgM_Ranking-RVDs	Primer pair generated using optimal parameters. 
D2
013-047528534R
GCTCCTGTCTAGCTTATCATCCTG
Tesson_IgM_Ranking-RVDs	Primer pair generated using optimal parameters.