Top Cleavage Sites for Tesson_IgM_Ranking-Homology

PROGNOS Summary for Tesson_IgM_Ranking-Homology_5TC

The rn4 genome was searched for the TALEN pair targeting:
5'- TTCCTGCCCAGCTCCAT NN..NN ACCAGAACAACACTGAA -3'

Up to 5 mismatches were allowed in each nuclease half-site.

Allowed spacing distances were: 12 13 14 15 16 17 18 19 20 21 22 23 24

Only heterodimers of the nuclease pair were included in the output

1973 total sites were located
Mismatch Types Genomic Regions
  0 and 0: 1
  3 and 3: 1
  2 and 5: 2
  3 and 4: 10
  3 and 5: 55
  4 and 4: 22
  4 and 5: 408
  5 and 5: 1474
  Exons: 35
  Promoters: 8
  Introns: 485
  Intergenic: 1445


The top 100 sites are ranked below according to a weighted sequence homology. All sites listed contain flanking 5' pyrimidines.
For the full output, click here.

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Primer pairs were designed using the 400 bp of sequence on either side of the cleavage site to melt close to 60 °C

For easy visualization of cleavage on gels:
The smallest fragment will be longer than 130 bp, which is also the minimum separation distance between uncleaved and cleaved products.
The two cleavage products will be separated by a minimum size of 0 bp
To optimize assay conditions, the amplicons have a minimum length of 260 bp, a maximum length of 450 bp, and an optimal length of 270 bp.
Rank: 1	Homology Score: 72
	Genomic Region: Intergenic	Closest Gene: Adam6	Caution! This site scores very closely to the intended nuclease target. The potential for cleavage at this site is of great concern.
Match Type: R17L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-ATGAC TTCAGTGTTGTTCTGGT    AGTTCCAGGAGAAGGAA     ATGGAGCTGGGCAGGAA GTCCC-3'
	3'-TACTG AAGTCACAACAAGACCA    TCAAGGTCCTCTTCCTT     TACCTCGACCCGTCCTT CAGGG-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 006-138443970
Forward Primer: 5'-GGAATCGGCACATGCAGATCTTTG-3' T_m: 59.038
Reverse Primer: 5'-TCTGGTCCTCAGAGAGTCAGTC-3' T_m: 59.258
Expected PCR Product Length: 325
Expected CelI Cleavage Product Sizes: 194, 131
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr6:138444137-138444187

Rank: 2	Homology Score: 18
	Genomic Region: Intron	Closest Gene: Pdcd11
Match Type: R16L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-TTGGA TGCAGACTTGTTCTGGT     CCTGTTAGCAGACCTC     AGGGATCTGGGGAGGAA TTAGC-3'
	3'-AACCT ACGTCTGAACAAGACCA     GGACAATCGTCTGGAG     TCCCTAGACCCCTCCTT AATCG-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 001-252428669
Forward Primer: 5'-GTTGTGTTTCAGGACAGCACTACC-3' T_m: 59.038
Reverse Primer: 5'-GTTGGAGAGGCCACATTCTTAACC-3' T_m: 59.038
Expected PCR Product Length: 308
Expected CelI Cleavage Product Sizes: 162, 146
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr1:252428804-252428853

Rank: 3	Homology Score: 17
	Genomic Region: Intergenic	Closest Gene: RGD1559903
Match Type: R16L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-TTCCA TGCAGTGTTGTTCTGTT     GTCAGTAATGGATGCC     AGAGAGTTGGGATGGAA TGAGA-3'
	3'-AAGGT ACGTCACAACAAGACAA     CAGTCATTACCTACGG     TCTCTCAACCCTACCTT ACTCT-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 020-016460972
Forward Primer: 5'-GTCAAGGACGAAGTCATCTGGAG-3' T_m: 59.148
Reverse Primer: 5'-GACAAGCTGACTGCTGCTAGAG-3' T_m: 59.258
Expected PCR Product Length: 340
Expected CelI Cleavage Product Sizes: 144, 196
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr20:16461089-16461138

Rank: 4	Homology Score: 17
	Genomic Region: Intergenic	Closest Gene: Lrfn2
Match Type: L24R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-TCCCC TTCCTGCCCAGCTCCCA TCCCCACCCCCTTCTGAGAACTAA ACAGGAAGCACACGGAA CCTGC-3'
	3'-AGGGG AAGGACGGGTCGAGGGT AGGGGTGGGGGAAGACTCTTGATT TGTCCTTCGTGTGCCTT GGACG-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 009-007190270
Forward Primer: 5'-AGTTGGGCTGGTAGAAATTGCTGG-3' T_m: 59.038
Reverse Primer: 5'-CTGAGTGAAACAAACGGCTCCGA-3' T_m: 59.148
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 133, 137
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr9:7190372-7190429

Rank: 5	Homology Score: 13
	Genomic Region: Promoter	Closest Gene: Cln5
Match Type: R14L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-AGTGT TTTATTGTTGTTATGGT      TTGGCTGGTGGTGG      CTGCAGGTGGGCAGAAA GCACC-3'
	3'-TCACA AAATAACAACAATACCA      AACCGACCACCACC      GACGTCCACCCGTCTTT CGTGG-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 015-087079625
Forward Primer: 5'-CAATCACTCCCTGCAATCCCC-3' T_m: 59.378
Reverse Primer: 5'-TGACGCCTCCACTGTCCTAG-3' T_m: 59.508
Expected PCR Product Length: 326
Expected CelI Cleavage Product Sizes: 142, 184
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr15:87079741-87079788

Rank: 6	Homology Score: 13
	Genomic Region: Intron	Closest Gene: Acvrl1
Match Type: R21L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-AAGGC TTCATTGAGGTTCTGGT  CTTCATGATCACAGAAGCGGC   CTGGAGTTGGGAAGGAG GAAGA-3'
	3'-TTCCG AAGTAACTCCAAGACCA  GAAGTACTAGTGTCTTCGCCG   GACCTCAACCCTTCCTC CTTCT-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 007-139879252
Forward Primer: 5'-AAAGGCTCATTAGACACGCTCAGG-3' T_m: 59.038
Reverse Primer: 5'-CACGTCTCTGCAGACCCCAT-3' T_m: 59.508
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 134, 136
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr7:139879357-139879411

Rank: 7	Homology Score: 13
	Genomic Region: Intron	Closest Gene: Ppm1l
Match Type: L12R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-ACTCT TTCCTCCCAAGCTGCTT       TTGGGCGGTTTT       ACCACAATAACAGTGAA GCAAA-3'
	3'-TGAGA AAGGAGGGTTCGACGAA       AACCCGCCAAAA       TGGTGTTATTGTCACTT CGTTT-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 002-159432579
Forward Primer: 5'-GCTAAATCCCCAACCCTGCAG-3' T_m: 59.378
Reverse Primer: 5'-GTGTTTACTTGCTCAAACACCCGG-3' T_m: 59.038
Expected PCR Product Length: 284
Expected CelI Cleavage Product Sizes: 154, 130
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr2:159432708-159432753

Rank: 8	Homology Score: 13
	Genomic Region: Intron	Closest Gene: Sars
Match Type: L22R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-GTAAG TACCTCCCCAGCTCAAT  TCCACTCACACAACCACAGATA  ACCAGGCCAAAACAGAA GTACC-3'
	3'-CATTC ATGGAGGGGTCGAGTTA  AGGTGAGTGTGTTGGTGTCTAT  TGGTCCGGTTTTGTCTT CATGG-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 002-204001007
Forward Primer: 5'-AATTTATGGGCTCTGTGCCGTGC-3' T_m: 59.148
Reverse Primer: 5'-CCCAGTGCTAAGTTGAGTGTGGC-3' T_m: 61.088
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 135, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr2:204001112-204001167

Rank: 9	Homology Score: 13
	Genomic Region: Intergenic	Closest Gene: Atpbd4
Match Type: R15L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-CATAA TTCAATGTTGTTTGGGC     TTTTTTTTTTTTTTT      TTGGAGCTGGGGACGAA CCCAG-3'
	3'-GTATT AAGTTACAACAAACCCG     AAAAAAAAAAAAAAA      AACCTCGACCCCTGCTT GGGTC-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 003-100952790
Forward Primer: 5'-GCCATGAAGAAGACATGCAGGAG-3' T_m: 59.148
Reverse Primer: 5'-GGATCACTTGAAATGTGGAGCTTCCTAC-3' T_m: 60.298
Expected PCR Product Length: 405
Expected CelI Cleavage Product Sizes: 218, 187
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr3:100952982-100953030

Rank: 10	Homology Score: 13
	Genomic Region: Intergenic	Closest Gene: Fgf13
Match Type: L18R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-CCTCG TTCCTGCTCTTCTCCAT    GGACCACTTTGATGAGAT    AAAAGAACAACACCCAA ACTGG-3'
	3'-GGAGC AAGGACGAGAAGAGGTA    CCTGGTGAAACTACTCTA    TTTTCTTGTTGTGGGTT TGACC-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 00X-144712285
Forward Primer: 5'-CCAAGCGAGACAGAGATCGG-3' T_m: 59.508
Reverse Primer: 5'-GGTACTTGGCCCTCAGAATCG-3' T_m: 59.378
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 135, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chrX:144712392-144712443

Rank: 11	Homology Score: 13
	Genomic Region: Intergenic	Closest Gene: Fgfr2
Match Type: L22R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-TTCTG CTCCACCCCAGCTCCAA  AGGAAATGGCTGCCCATAAATC  ACCAGAAAAACACCAAA GTCCC-3'
	3'-AAGAC GAGGTGGGGTCGAGGTT  TCCTTTACCGACGGGTATTTAG  TGGTCTTTTTGTGGTTT CAGGG-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 001-189623849
Forward Primer: 5'-TGCATTCCCACCCCCATACTG-3' T_m: 59.378
Reverse Primer: 5'-CTGAGCACATTCAGGCATCTGG-3' T_m: 59.258
Expected PCR Product Length: 297
Expected CelI Cleavage Product Sizes: 131, 166
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr1:189623950-189624005

Rank: 12	Homology Score: 13
	Genomic Region: Intergenic	Closest Gene: Ifnar1
Match Type: L15R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-CCTTG TTCCTTCCCAGCTGCCT     AGGAATGTGGGACCA      ACCTGAGCAACACAGCA AGAGC-3'
	3'-GGAAC AAGGAAGGGTCGACGGA     TCCTTACACCCTGGT      TGGACTCGTTGTGTCGT TCTCG-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 011-031478392
Forward Primer: 5'-GATCTGATCCCCTTTTCTGGTGTG-3' T_m: 59.038
Reverse Primer: 5'-CTTCTTTAGCACGTCGCCCC-3' T_m: 59.508
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 134, 136
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr11:31478500-31478548

Rank: 13	Homology Score: 13
	Genomic Region: Intergenic	Closest Gene: Tssc1
Match Type: L17R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-GGGGG CTCCTGCCCAGATCCTT    CTCTGCCAGGCTGCCCC     TCCAGCACAATATTGAA TCTCA-3'
	3'-CCCCC GAGGACGGGTCTAGGAA    GAGACGGTCCGACGGGG     AGGTCGTGTTATAACTT AGAGT-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 006-046986724
Forward Primer: 5'-CTTCTGGCTTAGTTTCCGTCTGTC-3' T_m: 59.038
Reverse Primer: 5'-CTCAGCTTGCCCAAGGGTTATAG-3' T_m: 59.148
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 130, 140
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr6:46986827-46986877

Rank: 14	Homology Score: 13
	Genomic Region: Intergenic	Closest Gene: Wdfy3
Match Type: L23R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-TTTCT TTCAGTCCCAGCTCCAT TTTTGTCCCTGCATTGCCTTTAG  ACAGGAACAACTCTGAG TTGCA-3'
	3'-AAAGA AAGTCAGGGTCGAGGTA AAAACAGGGACGTAACGGAAATC  TGTCCTTGTTGAGACTC AACGT-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 014-008802083
Forward Primer: 5'-TGCCAGGCTCCTGTCTGCAT-3' T_m: 59.508
Reverse Primer: 5'-GCTACCAGGACTCAATGGGG-3' T_m: 59.508
Expected PCR Product Length: 299
Expected CelI Cleavage Product Sizes: 139, 160
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr14:8802192-8802248

Rank: 15	Homology Score: 10
	Genomic Region: Exon	Closest Gene: Vom2r54
Match Type: R16L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-ATGAC CACAGTGGTGTTCAGTT     TATATCAGACTTGACA     AAGGAGCTGGACAAGAA TAAAA-3'
	3'-TACTG GTGTCACCACAAGTCAA     ATATAGTCTGAACTGT     TTCCTCGACCTGTTCTT ATTTT-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 007-014531040
Forward Primer: 5'-TCTCTGTATCAGGTGGCCCC-3' T_m: 59.508
Reverse Primer: 5'-CAAGAATCTCCCCATGATGAGGTG-3' T_m: 59.038
Expected PCR Product Length: 445
Expected CelI Cleavage Product Sizes: 132, 313
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr7:14531145-14531194

Rank: 16	Homology Score: 10
	Genomic Region: Exon	Closest Gene: Lrp1
Match Type: R20L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-GCTGT TTCAATCTTGGGGTGGT   TCCCCCAGTCTGACCAGTAC   ATGGTGCTGGGAAGGGA GAGAT-3'
	3'-CGACA AAGTTAGAACCCCACCA   AGGGGGTCAGACTGGTCATG   TACCACGACCCTTCCCT CTCTA-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 007-067524783
Forward Primer: 5'-TGATCAGAGCCTGCCCCTTC-3' T_m: 59.508
Reverse Primer: 5'-GACAGGGTGCTTCCTCACAATAC-3' T_m: 59.148
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 138, 132
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr7:67524892-67524945

Rank: 17	Homology Score: 10
	Genomic Region: Intron	Closest Gene: Pcbp3
Match Type: R21L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-AGGCC TTGAGTTTTGATCTGGT  CTCCCTTGATGGAAATAAAGC   AAGATGCTGGGCCAGAA TCCAT-3'
	3'-TCCGG AACTCAAAACTAGACCA  GAGGGAACTACCTTTATTTCG   TTCTACGACCCGGTCTT AGGTA-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 020-012161079
Forward Primer: 5'-CCGTTGCCCGAAAAGCACCT-3' T_m: 59.508
Reverse Primer: 5'-GAACCCATTTGCTCTCAGCCTTG-3' T_m: 59.148
Expected PCR Product Length: 320
Expected CelI Cleavage Product Sizes: 168, 152
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr20:12161218-12161272

Rank: 18	Homology Score: 10
	Genomic Region: Intron	Closest Gene: Tm6sf1
Match Type: R13L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-CTTTG TTCAGTTTTTTTTTTTT      TTTTTTTCTTTTT       TTGGAGCTGGGAACGAA CCCAG-3'
	3'-GAAAC AAGTCAAAAAAAAAAAA      AAAAAAAGAAAAA       AACCTCGACCCTTGCTT GGGTC-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. Warning! The sequence of this region of the genome is poorly mapped. 
Primer Index: 001-138061468
Forward Primer: 5'-CCGTGAAGCAGGTCACAAAGC-3' T_m: 59.378
Reverse Primer: 5'-CAGCAAGGGAAGTGCTACTTGATG-3' T_m: 59.038
Expected PCR Product Length: 303
Expected CelI Cleavage Product Sizes: 164, 139
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr1:138061607-138061653

Rank: 19	Homology Score: 10
	Genomic Region: Intron	Closest Gene: Tsc2
Match Type: L18R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-TTAGC TACCTGTCCAGCACCAT    GTCTGCCTGGTGCCTCCC    ACCATGACAATAATGGA CTAAA-3'
	3'-AATCG ATGGACAGGTCGTGGTA    CAGACGGACCACGGAGGG    TGGTACTGTTATTACCT GATTT-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 010-013862379
Forward Primer: 5'-CATAGGTGGGGCCTTATCAGAG-3' T_m: 59.258
Reverse Primer: 5'-GGTCCCTAGTCAAGGACATTGTCT-3' T_m: 59.038
Expected PCR Product Length: 273
Expected CelI Cleavage Product Sizes: 131, 142
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr10:13862482-13862533

Rank: 20	Homology Score: 10
	Genomic Region: Intron	Closest Gene: Cntn4
Match Type: L20R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-TTATC TACCTTCCCAGCCCCAT   TATGCATGGGTACAACATAA   ACCAACACAAAACAAAA GCAAA-3'
	3'-AATAG ATGGAAGGGTCGGGGTA   ATACGTACCCATGTTGTATT   TGGTTGTGTTTTGTTTT CGTTT-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 004-141883124
Forward Primer: 5'-AATCCGCAGTCTAAGCCAACAGAG-3' T_m: 59.038
Reverse Primer: 5'-TGCTAGGCAAGCACTCTACCAC-3' T_m: 59.258
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 134, 136
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr4:141883229-141883282

Rank: 21	Homology Score: 10
	Genomic Region: Intron	Closest Gene: Asip
Match Type: L22R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-AGGTT TCCCTGCGCTGCCTCAT  GATTAAGTTAACATTATTTTTA  GCAAGAACAACACAGAA GGGAT-3'
	3'-TCCAA AGGGACGCGACGGAGTA  CTAATTCAATTGTAATAAAAAT  CGTTCTTGTTGTGTCTT CCCTA-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 003-145456877
Forward Primer: 5'-GGCGACAGTTCCTCTGTTTTTGC-3' T_m: 59.148
Reverse Primer: 5'-CACAGTAGTGGCTGTAGGCATTTG-3' T_m: 59.038
Expected PCR Product Length: 375
Expected CelI Cleavage Product Sizes: 173, 202
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr3:145457020-145457075

Rank: 22	Homology Score: 10
	Genomic Region: Intron	Closest Gene: Mad1l1
Match Type: R13L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-GCAGC TTTAGTCTTGTTCTGTT      GTGATGTTTTGTT       TTGGAGATGCCAAGGAA CAAAC-3'
	3'-CGTCG AAATCAGAACAAGACAA      CACTACAAAACAA       AACCTCTACGGTTCCTT GTTTG-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 012-015035708
Forward Primer: 5'-AGCCTCCTGGTTACGTCCCAA-3' T_m: 59.378
Reverse Primer: 5'-TGACTGCTGTGTGTGGGGAG-3' T_m: 59.508
Expected PCR Product Length: 309
Expected CelI Cleavage Product Sizes: 137, 172
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr12:15035820-15035866

Rank: 23	Homology Score: 10
	Genomic Region: Intron	Closest Gene: Snx7
Match Type: R15L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-CAACT TTCTGTGTGGATATGGC     GATGACTAACTGGGC      ATGGAGCATGACAGGAA AAATT-3'
	3'-GTTGA AAGACACACCTATACCG     CTACTGATTGACCCG      TACCTCGTACTGTCCTT TTTAA-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 002-214232497
Forward Primer: 5'-CACGTGTGTGCCTCAGTAACAG-3' T_m: 59.258
Reverse Primer: 5'-AGCAGGCCCTGGAACAGTTCT-3' T_m: 59.378
Expected PCR Product Length: 310
Expected CelI Cleavage Product Sizes: 179, 131
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr2:214232650-214232698

Rank: 24	Homology Score: 10
	Genomic Region: Intron	Closest Gene: Fra10ac1
Match Type: L22R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-TCAAG TTCCTAGCCAGCTTCAT  TCACAGAGCAAGTTCCAGGACA  GCCAGGGCTACACAGAA AAACT-3'
	3'-AGTTC AAGGATCGGTCGAAGTA  AGTGTCTCGTTCAAGGTCCTGT  CGGTCCCGATGTGTCTT TTTGA-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 001-242536006
Forward Primer: 5'-TGGAGGTTGGGAGAGTAGAGG-3' T_m: 59.378
Reverse Primer: 5'-TGAAGGAGGGAACACTCAGCAG-3' T_m: 59.258
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 135, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr1:242536111-242536166

Rank: 25	Homology Score: 10
	Genomic Region: Intron	Closest Gene: Sorcs1
Match Type: L23R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-TGATG CTGCTGCTCAACTCCCT TGCTAACAACTGAATACATCTGT  ATCAGAACAACAGTGAG CAAAA-3'
	3'-ACTAC GACGACGAGTTGAGGGA ACGATTGTTGACTTATGTAGACA  TAGTCTTGTTGTCACTC GTTTT-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 001-256112389
Forward Primer: 5'-CCTGGATAAGTCAGTAAAGGGTGG-3' T_m: 59.038
Reverse Primer: 5'-CCTTGGTGGGACCTGATACAGT-3' T_m: 59.258
Expected PCR Product Length: 353
Expected CelI Cleavage Product Sizes: 204, 149
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr1:256112563-256112619

Rank: 26	Homology Score: 10
	Genomic Region: Intron	Closest Gene: Ube2j1
Match Type: L21R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-TTGCT TTCCTTTCCAGCTCCTT  CTGCCTCTCTTAGGCATTTCC   AGCAGAGCCACTCTGCA ATGGT-3'
	3'-AACGA AAGGAAAGGTCGAGGAA  GACGGAGAGAATCCGTAAAGG   TCGTCTCGGTGAGACGT TACCA-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 005-049320556
Forward Primer: 5'-CTGTGGTGAAGGTGTTACTGTGTC-3' T_m: 59.038
Reverse Primer: 5'-GAGAAGGCTGGACCGAATAGAAG-3' T_m: 59.148
Expected PCR Product Length: 292
Expected CelI Cleavage Product Sizes: 161, 131
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr5:49320688-49320742

Rank: 27	Homology Score: 10
	Genomic Region: Intron	Closest Gene: Ralgapa1
Match Type: L13R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-TCTCC TTCCTTTCCAGCTCCCT      TCTTTCTTTCATT       ACTAGATATGCACTGAA AGCTG-3'
	3'-AGAGG AAGGAAAGGTCGAGGGA      AGAAAGAAAGTAA       TGATCTATACGTGACTT TCGAC-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 006-075907048
Forward Primer: 5'-GGTGTCCTCATTCACCTCTGACAA-3' T_m: 59.038
Reverse Primer: 5'-GTGTTTGGAACATCCTCTGCGAC-3' T_m: 59.148
Expected PCR Product Length: 363
Expected CelI Cleavage Product Sizes: 189, 174
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr6:75907212-75907258

Rank: 28	Homology Score: 10
	Genomic Region: Intron	Closest Gene: Rnf4
Match Type: L19R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-GTAGA TCCCTCCCCAGCTCCCT   TGTCAGGACCTAGCCAGGT    ACATGATCAACATTCAA GAAAA-3'
	3'-CATCT AGGGAGGGGTCGAGGGA   ACAGTCCTGGATCGGTCCA    TGTACTAGTTGTAAGTT CTTTT-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 014-082098705
Forward Primer: 5'-AGCCAAGGAAGAAACCTGTGCAGC-3' T_m: 60.908
Reverse Primer: 5'-CTGTGGATGGCTAGAGTGTACTCA-3' T_m: 59.038
Expected PCR Product Length: 300
Expected CelI Cleavage Product Sizes: 130, 170
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr14:82098807-82098859

Rank: 29	Homology Score: 10
	Genomic Region: Intron	Closest Gene: Emid1
Match Type: L15R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-AACAT TCCCTGTCCAGCTCCAC     CTCTTGGAAACCTTG      AGGAAAACAACCCTGCA GACTT-3'
	3'-TTGTA AGGGACAGGTCGAGGTG     GAGAACCTTTGGAAC      TCCTTTTGTTGGGACGT CTGAA-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 014-085768096
Forward Primer: 5'-GGGAATGGTTAGCCCCATCTC-3' T_m: 59.378
Reverse Primer: 5'-CATGAGGAAACAGTTCAGGAGGG-3' T_m: 59.148
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 136, 134
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr14:85768206-85768254

Rank: 30	Homology Score: 10
	Genomic Region: Intergenic	Closest Gene: Tacstd2
Match Type: L24R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-TTTCC TTCATTCTCTGCTTCAT TCATTGTGTCTGCATTTCCTTTAG ACAGGAACAACTCTGAA TTATT-3'
	3'-AAAGG AAGTAAGAGACGAAGTA AGTAACACAGACGTAAAGGAAATC TGTCCTTGTTGAGACTT AATAA-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 004-100499585
Forward Primer: 5'-GGCATAGCCTCTCAGGAAACAG-3' T_m: 59.258
Reverse Primer: 5'-CTTCCAGGACAGAACAGGAAAGAC-3' T_m: 59.038
Expected PCR Product Length: 336
Expected CelI Cleavage Product Sizes: 165, 171
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr4:100499719-100499776

Rank: 31	Homology Score: 10
	Genomic Region: Intergenic	Closest Gene: Tbl1xr1
Match Type: R22L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-ACATC TCCAGTCTTATTCTGGT  CTATTATCAACAGTAAGGGAAA  ATGAAGCTGCCATGGAA AGTAC-3'
	3'-TGTAG AGGTCAGAATAAGACCA  GATAATAGTTGTCATTCCCTTT  TACTTCGACGGTACCTT TCATG-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 002-106965669
Forward Primer: 5'-ATAGTGGCCAGTGCCTGGAC-3' T_m: 59.508
Reverse Primer: 5'-CCAGGAGAAAAGGCCATCTTCCA-3' T_m: 59.148
Expected PCR Product Length: 307
Expected CelI Cleavage Product Sizes: 131, 176
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr2:106965770-106965825

Rank: 32	Homology Score: 10
	Genomic Region: Intergenic	Closest Gene: MGC125002
Match Type: R23L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-TTTGA TCCAGAATTGTTCTGGT CTAAAAGAAATGTGGTGATAAAA  ATGGAGCAGAGATTGAA GGAAA-3'
	3'-AAACT AGGTCTTAACAAGACCA GATTTTCTTTACACCACTATTTT  TACCTCGTCTCTAACTT CCTTT-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 005-113632907
Forward Primer: 5'-GGAGGAATCTCTAGAAAGTCCCAG-3' T_m: 59.038
Reverse Primer: 5'-GGCTCCTATCTGCAGGTCCA-3' T_m: 59.508
Expected PCR Product Length: 339
Expected CelI Cleavage Product Sizes: 208, 131
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr5:113633085-113633141

Rank: 33	Homology Score: 10
	Genomic Region: Intergenic	Closest Gene: LOC688539
Match Type: R18L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-CCAGT TTGGGTGTTTTTCTTTT    ATCTGGTCAAAGTGATCC    ATGGAGAAGAGCAGGAA TGAGG-3'
	3'-GGTCA AACCCACAAAAAGAAAA    TAGACCAGTTTCACTAGG    TACCTCTTCTCGTCCTT ACTCC-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 00X-117110415
Forward Primer: 5'-CTTTGCCCACAGAATAGCAGCC-3' T_m: 59.258
Reverse Primer: 5'-CTAGCCAAGCGCGACAAAGTTC-3' T_m: 59.258
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 135, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chrX:117110522-117110573

Rank: 34	Homology Score: 10
	Genomic Region: Intergenic	Closest Gene: Galc
Match Type: R24L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-CTTGT CTCTGTGTTGCTCTGGT ATACAGACACAATCTAGAAGCTTC AGGAAGATGGGCAGATA TGCTA-3'
	3'-GAACA GAGACACAACGAGACCA TATGTCTGTGTTAGATCTTCGAAG TCCTTCTACCCGTCTAT ACGAT-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 006-122127331
Forward Primer: 5'-GATATTGGGAGACCCCCTAACTCA-3' T_m: 59.038
Reverse Primer: 5'-GGGCATGACCCAACTACAATTACC-3' T_m: 59.038
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 131, 139
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr6:122127431-122127488

Rank: 35	Homology Score: 10
	Genomic Region: Intergenic	Closest Gene: Pde4b
Match Type: R15L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-TTTCG TCCATTATTGTCATGGT     GTCATGCAAGGTGAC      ATGGTGCTGGGGAGGCA GCTGA-3'
	3'-AAAGC AGGTAATAACAGTACCA     CAGTACGTTCCACTG      TACCACGACCCCTCCGT CGACT-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 005-123636672
Forward Primer: 5'-CTCTGAGTGACACGAATTAGTGGG-3' T_m: 59.038
Reverse Primer: 5'-GGAGTGTGTCACTAAGGCTGG-3' T_m: 59.378
Expected PCR Product Length: 350
Expected CelI Cleavage Product Sizes: 218, 132
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr5:123636864-123636912

Rank: 36	Homology Score: 10
	Genomic Region: Intergenic	Closest Gene: Mctp2
Match Type: R14L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-AATCA TTCAGTGAATTTGTTGT      GTCACATCTGCCCC      ATGGAGGTGGGAGGGAA GAATA-3'
	3'-TTAGT AAGTCACTTAAACAACA      CAGTGTAGACGGGG      TACCTCCACCCTCCCTT CTTAT-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 001-127719260
Forward Primer: 5'-CCAACATTCTCCCAAGAGATTCTGGAAAC-3' T_m: 60.178
Reverse Primer: 5'-GTTCCAGGTCAACCAGGACTAC-3' T_m: 59.258
Expected PCR Product Length: 368
Expected CelI Cleavage Product Sizes: 135, 233
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr1:127719369-127719416

Rank: 37	Homology Score: 10
	Genomic Region: Intergenic	Closest Gene: Ocrl
Match Type: R18L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-CCAGT TTGGGTGTTGTTCTTTC    AGCTGGTCAAAGTGGTCC    ATGGAGAAGAGCAGGAA TGAGG-3'
	3'-GGTCA AACCCACAACAAGAAAG    TCGACCAGTTTCACCAGG    TACCTCTTCTCGTCCTT ACTCC-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 00X-133545933
Forward Primer: 5'-CGCTCTTCAAGGTATTTTGCCCAC-3' T_m: 59.038
Reverse Primer: 5'-CTAGCCAAACACGACAAAGATCGG-3' T_m: 59.038
Expected PCR Product Length: 281
Expected CelI Cleavage Product Sizes: 149, 132
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chrX:133546054-133546105

Rank: 38	Homology Score: 10
	Genomic Region: Intergenic	Closest Gene: Zfand6
Match Type: R15L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-AGGAG TTCAGTCATGTTCAAGG     GCACTTCTTGTGTAA      ATGGGGCTGGACAGGGA GGAGT-3'
	3'-TCCTC AAGTCAGTACAAGTTCC     CGTGAAGAACACATT      TACCCCGACCTGTCCCT CCTCA-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 001-140972716
Forward Primer: 5'-GGGACCACCTCAGCTAAGGA-3' T_m: 59.508
Reverse Primer: 5'-GTGGGGTTGTTGGTTTTCAGCTC-3' T_m: 59.148
Expected PCR Product Length: 368
Expected CelI Cleavage Product Sizes: 173, 195
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr1:140972863-140972911

Rank: 39	Homology Score: 10
	Genomic Region: Intergenic	Closest Gene: Mir3589
Match Type: R22L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-CTTTG TAGACTGTGGTTCTGCT  GATTGTGGGATGACAGAAAGGA  ATGGAGATGGGCTAGAA GTCCA-3'
	3'-GAAAC ATCTGACACCAAGACGA  CTAACACCCTACTGTCTTTCCT  TACCTCTACCCGATCTT CAGGT-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 00X-146103263
Forward Primer: 5'-CTACACTCCTTTTTGTTGGGTGGG-3' T_m: 59.038
Reverse Primer: 5'-CTCTGCTGCAGAGCCTTTTTTCC-3' T_m: 59.148
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 136, 134
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chrX:146103369-146103424

Rank: 40	Homology Score: 10
	Genomic Region: Intergenic	Closest Gene: Mafb
Match Type: R16L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-CTTTC TGCTGTGTTTTTCTGGT     TTCATTTGGATGTGAG     ATGGAGCTGAACTGTGA TTTCA-3'
	3'-GAAAG ACGACACAAAAAGACCA     AAGTAAACCTACACTC     TACCTCGACTTGACACT AAAGT-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 003-150734535
Forward Primer: 5'-CACCAGCCAAGAACGGCGTCTTT-3' T_m: 61.088
Reverse Primer: 5'-CACCTCTGCACCTCCTTCTAAC-3' T_m: 59.258
Expected PCR Product Length: 323
Expected CelI Cleavage Product Sizes: 131, 192
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr3:150734639-150734688

Rank: 41	Homology Score: 10
	Genomic Region: Intergenic	Closest Gene: Uncx
Match Type: L23R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-GAGTC TTTCTGCCCAGCTCTAG CTGTCCTGGAACTCACCCTGTAG  ACCAGGCCAGCCTTGAA CTCAC-3'
	3'-CTCAG AAAGACGGGTCGAGATC GACAGGACCTTGAGTGGGACATC  TGGTCCGGTCGGAACTT GAGTG-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 012-015478690
Forward Primer: 5'-CCACTCAAGACAAGCAGAAAGGAC-3' T_m: 59.038
Reverse Primer: 5'-CAAAACAGCAGCAGCAAAAGCTGG-3' T_m: 59.038
Expected PCR Product Length: 278
Expected CelI Cleavage Product Sizes: 147, 131
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr12:15478807-15478863

Rank: 42	Homology Score: 10
	Genomic Region: Intergenic	Closest Gene: Olr98
Match Type: L15R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-TGGGT TTAGTCCCCAGCTCCTA     AAAAAAAAAAAAAGA      ACCAAAACAACTCTGAG GCTCC-3'
	3'-ACCCA AATCAGGGGTCGAGGAT     TTTTTTTTTTTTTCT      TGGTTTTGTTGAGACTC CGAGG-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 001-161161633
Forward Primer: 5'-CTGCTGAGGAATCTCAAATGGCTG-3' T_m: 59.038
Reverse Primer: 5'-CATTTCCACCAGCAATGGAGGAG-3' T_m: 59.148
Expected PCR Product Length: 317
Expected CelI Cleavage Product Sizes: 185, 132
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr1:161161792-161161840

Rank: 43	Homology Score: 10
	Genomic Region: Intergenic	Closest Gene: Fundc1
Match Type: L19R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-AGACC TTCCTAACCCTTTCCAT   TTGCTTAAATTAACAGAGA    AACAGAACAATACTCAA AATTG-3'
	3'-TCTGG AAGGATTGGGAAAGGTA   AACGAATTTAATTGTCTCT    TTGTCTTGTTATGAGTT TTAAC-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 00X-016509931
Forward Primer: 5'-CTACCAATGTCTATGGAGTGACCG-3' T_m: 59.038
Reverse Primer: 5'-GCCTAGAGTATCCCAAATGTGCAC-3' T_m: 59.038
Expected PCR Product Length: 365
Expected CelI Cleavage Product Sizes: 130, 235
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chrX:16510033-16510085

Rank: 44	Homology Score: 10
	Genomic Region: Intergenic	Closest Gene: LOC679825
Match Type: R16L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-TGAGG TTTACTTTTGCTGTGGT     AAAACATCATGACCAA     AGGGACTTGGGCAGGAA AGAGT-3'
	3'-ACTCC AAATGAAAACGACACCA     TTTTGTAGTACTGGTT     TCCCTGAACCCGTCCTT TCTCA-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 001-001765937
Forward Primer: 5'-TTCTAGCCAAGAGGTCCAAAGGCC-3' T_m: 60.908
Reverse Primer: 5'-TGCATGGCCTCTGCATCAGC-3' T_m: 59.508
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 135, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr1:1766045-1766094

Rank: 45	Homology Score: 10
	Genomic Region: Intergenic	Closest Gene: Mir206
Match Type: L23R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-TTTTA TTAGTGCCCAAATCTAT AGTAGTAATGGTCTAATTTGAGC  TCCAGAATAACTCTGAA TCAAA-3'
	3'-AAAAT AATCACGGGTTTAGATA TCATCATTACCAGATTAAACTCG  AGGTCTTATTGAGACTT AGTTT-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 009-019047855
Forward Primer: 5'-GTGGCTTTGGTCCTCATCTGTC-3' T_m: 59.258
Reverse Primer: 5'-CCTCTTTCGGAGATAGGCATGTTC-3' T_m: 59.038
Expected PCR Product Length: 302
Expected CelI Cleavage Product Sizes: 161, 141
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr9:19047986-19048042

Rank: 46	Homology Score: 10
	Genomic Region: Intergenic	Closest Gene: Rn45s
Match Type: L23R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-GGATC TGCCTTCTCAGATCCAA ATCCTCCATTTCAAGATGCAAGA  AGCAGAGCCACACTGAA AGCTG-3'
	3'-CCTAG ACGGAAGAGTCTAGGTT TAGGAGGTAAAGTTCTACGTTCT  TCGTCTCGGTGTGACTT TCGAC-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 0Un-021787610
Forward Primer: 5'-CCTGGGATGTGGTGTAAGTTGG-3' T_m: 59.258
Reverse Primer: 5'-GAGGAGCCAAATCTGGACCATTAG-3' T_m: 59.038
Expected PCR Product Length: 273
Expected CelI Cleavage Product Sizes: 138, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chrUn:21787718-21787774

Rank: 47	Homology Score: 10
	Genomic Region: Intergenic	Closest Gene: Cntnap5b
Match Type: L22R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-CTCTT TCTCTTCCTAGCTCCCT  GCAATTGTATCCTGCTGTGAGT  ACCAGATCAAAAGTGAA TATTT-3'
	3'-GAGAA AGAGAAGGATCGAGGGA  CGTTAACATAGGACGACACTCA  TGGTCTAGTTTTCACTT ATAAA-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 013-002441765
Forward Primer: 5'-CTGTCTCTGTCTCTCTTAGTTCCC-3' T_m: 59.038
Reverse Primer: 5'-CCCTTTTTGCCTTTCCATGCAGTG-3' T_m: 59.038
Expected PCR Product Length: 336
Expected CelI Cleavage Product Sizes: 177, 159
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr13:2441912-2441967

Rank: 48	Homology Score: 10
	Genomic Region: Intergenic	Closest Gene: Mybpc1
Match Type: R22L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-GGTCT TTCTGTGTAGTCCTGGT  TGTCTTGGAATTCTCTATGTAG  ATCAAGCTGGCCCGAAA CTCAT-3'
	3'-CCAGA AAGACACATCAGGACCA  ACAGAACCTTAAGAGATACATC  TAGTTCGACCGGGCTTT GAGTA-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 007-025308852
Forward Primer: 5'-CCAGTCTGATGGTTATACATACAGTGCAGT-3' T_m: 60.058
Reverse Primer: 5'-GGTTTTCAACCTGAGTGGGAGAAG-3' T_m: 59.038
Expected PCR Product Length: 358
Expected CelI Cleavage Product Sizes: 220, 138
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr7:25309042-25309097

Rank: 49	Homology Score: 10
	Genomic Region: Intergenic	Closest Gene: Mir532
Match Type: L21R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-TTTTT TTCCTACCCACCCCCAT  GCCTCCCCAGCCTTGGATGAA   ACAGGAACAAAAGTCAA AATTT-3'
	3'-AAAAA AAGGATGGGTGGGGGTA  CGGAGGGGTCGGAACCTACTT   TGTCCTTGTTTTCAGTT TTAAA-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 00X-027254372
Forward Primer: 5'-CCATTATCGCTAGCCTAGCTTACG-3' T_m: 59.038
Reverse Primer: 5'-GACTGGGAATAAGTGGTGTGGTAC-3' T_m: 59.038
Expected PCR Product Length: 301
Expected CelI Cleavage Product Sizes: 167, 134
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chrX:27254510-27254564

Rank: 50	Homology Score: 10
	Genomic Region: Intergenic	Closest Gene: Cldn8
Match Type: R21L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-CTCTG TTCTGTGTTCTGTTGGG  AGTTAAATCAAACGTATTTTC   ATGGAGCTTGGCATGTA GAAAA-3'
	3'-GAGAC AAGACACAAGACAACCC  TCAATTTAGTTTGCATAAAAG   TACCTCGAACCGTACAT CTTTT-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 011-028431745
Forward Primer: 5'-GCTCAGAACAGCTAATGTGAGTGC-3' T_m: 59.038
Reverse Primer: 5'-CAATGACTGTCCAGAACTGAGGC-3' T_m: 59.148
Expected PCR Product Length: 314
Expected CelI Cleavage Product Sizes: 177, 137
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr11:28431893-28431947

Rank: 51	Homology Score: 10
	Genomic Region: Intergenic	Closest Gene: Tmem132b
Match Type: R24L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-GTTGT TTTGTTGTTGTTTTGTT TTTTTTGTTTTGTTTTTTAGATTC ATTTAGCTGGACAGGAA GAGTA-3'
	3'-CAACA AAACAACAACAAAACAA AAAAAACAAAACAAAAAATCTAAG TAAATCGACCTGTCCTT CTCAT-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 012-031781347
Forward Primer: 5'-CGCGTAATACGCCTTTTGTTGACC-3' T_m: 59.038
Reverse Primer: 5'-GAGCAGTGAACAGCATTAGCTTCC-3' T_m: 59.038
Expected PCR Product Length: 368
Expected CelI Cleavage Product Sizes: 232, 136
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr12:31781548-31781605

Rank: 52	Homology Score: 10
	Genomic Region: Intergenic	Closest Gene: Cav2
Match Type: L23R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-GGATC TGCCTTCTCAGATCCAA ATCCTCCTTTTCAAGATGCAAGA  AGCAGAGCCACACTGAA AGCTG-3'
	3'-CCTAG ACGGAAGAGTCTAGGTT TAGGAGGAAAAGTTCTACGTTCT  TCGTCTCGGTGTGACTT TCGAC-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: dom-003376644
Forward Primer: 5'-CCTGGGATGTGGTGTAAGTTGG-3' T_m: 59.258
Reverse Primer: 5'-GAGGAGCCAAATCTGGACCATTAG-3' T_m: 59.038
Expected PCR Product Length: 273
Expected CelI Cleavage Product Sizes: 138, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chrUn_random:3376752-3376808

Rank: 53	Homology Score: 10
	Genomic Region: Intergenic	Closest Gene: Dpp6
Match Type: R15L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-GTCAG TTCTGTGCTGTGCTCAT     GCCATTGTGGGGAAG      AAGGAGATGGGAAGGAA GGTGC-3'
	3'-CAGTC AAGACACGACACGAGTA     CGGTAACACCCCTTC      TTCCTCTACCCTTCCTT CCACG-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 004-003843423
Forward Primer: 5'-GGTGACCAATACCAACCATCAGG-3' T_m: 59.148
Reverse Primer: 5'-CCTCCTTTCAGGAAGGCTCTTTCT-3' T_m: 59.038
Expected PCR Product Length: 319
Expected CelI Cleavage Product Sizes: 169, 150
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr4:3843566-3843614

Rank: 54	Homology Score: 10
	Genomic Region: Intergenic	Closest Gene: LOC367515
Match Type: L23R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-GGATC TGCCTTCTCAGATCCAA ATCCTCCATTTCAAGATGCAAGA  AGCAGAGCCACACTGAA AGCTG-3'
	3'-CCTAG ACGGAAGAGTCTAGGTT TAGGAGGTAAAGTTCTACGTTCT  TCGTCTCGGTGTGACTT TCGAC-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. Warning! The sequence of this region of the genome is poorly mapped. 
Primer Index: 0Un-038608458
Forward Primer: 5'-CCTGGGATGTGGTGTAAGTTGG-3' T_m: 59.258
Reverse Primer: 5'-GAGACAACATACCCTGCTCTCAC-3' T_m: 59.148
Expected PCR Product Length: 316
Expected CelI Cleavage Product Sizes: 138, 178
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chrUn:38608566-38608622

Rank: 55	Homology Score: 10
	Genomic Region: Intergenic	Closest Gene: Gabra2
Match Type: R19L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-GCTGT TGCAGTTTAGTATTGGT   ACGGAGGTCCTGGGAAGGC    ATTGCTCTGGGCAGGAA ATAAG-3'
	3'-CGACA ACGTCAAATCATAACCA   TGCCTCCAGGACCCTTCCG    TAACGAGACCCGTCCTT TATTC-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 014-039438747
Forward Primer: 5'-GCTCCATAAACTGGGCCTGAGA-3' T_m: 59.258
Reverse Primer: 5'-GCTAGCCTCTACCTCTTCCTG-3' T_m: 59.378
Expected PCR Product Length: 342
Expected CelI Cleavage Product Sizes: 144, 198
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr14:39438863-39438915

Rank: 56	Homology Score: 10
	Genomic Region: Intergenic	Closest Gene: E2f3
Match Type: R20L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-TTTTG TGCTGTGCTGTGCTGGG   CACAGAACCCAGGGCAGAGG   AGCGTGCTGGGCAGGAA CCCAA-3'
	3'-AAAAC ACGACACGACACGACCC   GTGTCTTGGGTCCCGTCTCC   TCGCACGACCCGTCCTT GGGTT-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 017-041218534
Forward Primer: 5'-CCAGGCCATGATTATTCTGCAGG-3' T_m: 59.148
Reverse Primer: 5'-TCCCAAGTCACCTGCAAGCC-3' T_m: 59.508
Expected PCR Product Length: 343
Expected CelI Cleavage Product Sizes: 212, 131
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr17:41218717-41218770

Rank: 57	Homology Score: 10
	Genomic Region: Intergenic	Closest Gene: Grxcr1
Match Type: L22R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-TACTT TTCCCGTCCACCTCCAT  GTGTTGATTCTTGCTGTTCAAA  AACAAAACAAAACAAAA CAAAA-3'
	3'-ATGAA AAGGGCAGGTGGAGGTA  CACAACTAAGAACGACAAGTTT  TTGTTTTGTTTTGTTTT GTTTT-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 014-042588777
Forward Primer: 5'-GAGACTACAGATGCCAAAAGTTTCCATACC-3' T_m: 60.058
Reverse Primer: 5'-ACACACACACACACACACACACAC-3' T_m: 59.038
Expected PCR Product Length: 411
Expected CelI Cleavage Product Sizes: 281, 130
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr14:42589028-42589083

Rank: 58	Homology Score: 10
	Genomic Region: Intergenic	Closest Gene: Plcl2
Match Type: R21L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-ATGTT TTCAGTGTTGCTCCTGT  TGGGTAAAGGAGGAATGTTCC   CTGGATCGGGGCTGGTA TAGTG-3'
	3'-TACAA AAGTCACAACGAGGACA  ACCCATTTCCTCCTTACAAGG   GACCTAGCCCCGACCAT ATCAC-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 009-004673112
Forward Primer: 5'-AATCCACCGAGCCTAGTAACTGC-3' T_m: 59.148
Reverse Primer: 5'-TACACTGCGCTCACACAGCAAG-3' T_m: 59.258
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 135, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr9:4673218-4673272

Rank: 59	Homology Score: 10
	Genomic Region: Intergenic	Closest Gene: Adrbk2
Match Type: L23R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-GGATC TGCCTTCTCAGATCCAA ATCCTCCATTTCAAGATGCAAGA  AGCAGAGCCACACTGAA AGCTG-3'
	3'-CCTAG ACGGAAGAGTCTAGGTT TAGGAGGTAAAGTTCTACGTTCT  TCGTCTCGGTGTGACTT TCGAC-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 0Un-051334472
Forward Primer: 5'-CCTGGGATGTGGTGTAAGTTGG-3' T_m: 59.258
Reverse Primer: 5'-GAGGAGCCAAATCTGGACCATTAG-3' T_m: 59.038
Expected PCR Product Length: 273
Expected CelI Cleavage Product Sizes: 138, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chrUn:51334580-51334636

Rank: 60	Homology Score: 10
	Genomic Region: Intergenic	Closest Gene: Cbx8
Match Type: R20L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-AAACA TTCAGTGTTGGACTGGC   AAGATGGCTCACTGGGTGAA   AGGGAGCTTGCCACCAA GCTTG-3'
	3'-TTTGT AAGTCACAACCTGACCG   TTCTACCGAGTGACCCACTT   TCCCTCGAACGGTGGTT CGAAC-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 0Un-051940753
Forward Primer: 5'-CAAACAGAGCCAGGTGTGGTG-3' T_m: 59.378
Reverse Primer: 5'-GCCCTCTGTGTCATGCCTTATTAC-3' T_m: 59.038
Expected PCR Product Length: 355
Expected CelI Cleavage Product Sizes: 163, 192
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chrUn:51940887-51940940

Rank: 61	Homology Score: 10
	Genomic Region: Intergenic	Closest Gene: Smoc2
Match Type: R18L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-CTGGC TTCAGTGTGCTTCTGGG    GTGGCTACAGTTGCTCAC    CTGAAGCTGGGGAAGAG GCATG-3'
	3'-GACCG AAGTCACACGAAGACCC    CACCGATGTCAACGAGTG    GACTTCGACCCCTTCTC CGTAC-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 001-053325789
Forward Primer: 5'-ACATGGCTTCCATACCGAGGC-3' T_m: 59.378
Reverse Primer: 5'-GAAGGGGTTCTTTGCGCCAAATAG-3' T_m: 59.038
Expected PCR Product Length: 296
Expected CelI Cleavage Product Sizes: 155, 141
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr1:53325916-53325967

Rank: 62	Homology Score: 10
	Genomic Region: Intergenic	Closest Gene: Pard3
Match Type: R12L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-TTTGC TTTTTTATTGTTCTGGA       AGTTAAGTTTGA       ATGGGGCTTGGTAGGAA ATGAT-3'
	3'-AAACG AAAAAATAACAAGACCT       TCAATTCAAACT       TACCCCGAACCATCCTT TACTA-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 019-057896612
Forward Primer: 5'-CCCAGTGCTGGGGTTACAAACA-3' T_m: 59.258
Reverse Primer: 5'-TCTTAGCCAGGAATGAGGCTGC-3' T_m: 59.258
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 138, 132
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr19:57896725-57896770

Rank: 63	Homology Score: 10
	Genomic Region: Intergenic	Closest Gene: Zfp385b
Match Type: L19R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-TTTCC TTCCAGCCCATCTCCAC   TTCTTTATGCCAGCACTGA    TCCACAGAAACACTGTA AACCA-3'
	3'-AAAGG AAGGTCGGGTAGAGGTG   AAGAAATACGGTCGTGACT    AGGTGTCTTTGTGACAT TTGGT-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 003-060396362
Forward Primer: 5'-CCACTGGGAGTGTATTCGGTATG-3' T_m: 59.148
Reverse Primer: 5'-GTAAACAGGCCTCTACAGAACGG-3' T_m: 59.148
Expected PCR Product Length: 362
Expected CelI Cleavage Product Sizes: 230, 132
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr3:60396564-60396616

Rank: 64	Homology Score: 10
	Genomic Region: Intergenic	Closest Gene: LOC302022
Match Type: L23R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-GGATC TGCCTTCTCAGATCCAA ATCCTCCATTTCAAGATGCAAGA  AGCAGAGCCACACTGAA AGCTG-3'
	3'-CCTAG ACGGAAGAGTCTAGGTT TAGGAGGTAAAGTTCTACGTTCT  TCGTCTCGGTGTGACTT TCGAC-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 0Un-061148009
Forward Primer: 5'-CCTGGGATGTGGTGTAAGTTGG-3' T_m: 59.258
Reverse Primer: 5'-GAGACAACATACCCTGCTCTCAC-3' T_m: 59.148
Expected PCR Product Length: 316
Expected CelI Cleavage Product Sizes: 138, 178
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chrUn:61148117-61148173

Rank: 65	Homology Score: 10
	Genomic Region: Intergenic	Closest Gene: Map2
Match Type: R13L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-ATTTA TTCATTGTTGTTCTTGG      TGGCTGCGTTATC       AGTGTTCTGGTCAGGAA GTTGT-3'
	3'-TAAAT AAGTAACAACAAGAACC      ACCGACGCAATAG       TCACAAGACCAGTCCTT CAACA-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 009-065106893
Forward Primer: 5'-CCTCTGTTGGATGTGGAGTTGG-3' T_m: 59.258
Reverse Primer: 5'-CGCACACCTATGGACATCTGAG-3' T_m: 59.258
Expected PCR Product Length: 396
Expected CelI Cleavage Product Sizes: 137, 259
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr9:65107005-65107051

Rank: 66	Homology Score: 10
	Genomic Region: Intergenic	Closest Gene: Irx1
Match Type: R22L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-GGTTT TTCTGTGTAGTTCTGGC  TGTCCTGGAACTGAATCAGTAG  ATGAGGCTGGCCTTGAA CTCAA-3'
	3'-CCAAA AAGACACATCAAGACCG  ACAGGACCTTGACTTAGTCATC  TACTCCGACCGGAACTT GAGTT-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 0Un-075352159
Forward Primer: 5'-CTTGAGAGACTGTGGTGAATGAGG-3' T_m: 59.038
Reverse Primer: 5'-GGAGGAAAAAGGGGACTGTGTTCA-3' T_m: 59.038
Expected PCR Product Length: 298
Expected CelI Cleavage Product Sizes: 150, 148
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chrUn:75352279-75352334

Rank: 67	Homology Score: 10
	Genomic Region: Intergenic	Closest Gene: Grid2
Match Type: R22L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-GGTTT TTCTGTGTAGTTCTGGC  TGTCCTGGAACTGAATCAGTAG  ATGAGGCTGGCCTTGAA CTCAA-3'
	3'-CCAAA AAGACACATCAAGACCG  ACAGGACCTTGACTTAGTCATC  TACTCCGACCGGAACTT GAGTT-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 004-092024652
Forward Primer: 5'-CTTGAGAGACTGTGGTGAATGAGG-3' T_m: 59.038
Reverse Primer: 5'-GGAGGAAAAAGGGGACTGTGTTCA-3' T_m: 59.038
Expected PCR Product Length: 298
Expected CelI Cleavage Product Sizes: 150, 148
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr4:92024772-92024827

Rank: 68	Homology Score: 10
	Genomic Region: Intergenic	Closest Gene: Mir384
Match Type: L18R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-CCTCA TTCCTGCTCTTCTCCAT    GGATCACTTTGACCAGAT    AAAAGAAGAACACCCAA ATTGG-3'
	3'-GGAGT AAGGACGAGAAGAGGTA    CCTAGTGAAACTGGTCTA    TTTTCTTCTTGTGGGTT TAACC-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 00X-093585607
Forward Primer: 5'-CCAAGCGCGACAAAGATCGG-3' T_m: 59.508
Reverse Primer: 5'-GGTACTTTGCCCACAGAATAGCAG-3' T_m: 59.038
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 135, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chrX:93585714-93585765

Rank: 69	Homology Score: 10
	Genomic Region: Intergenic	Closest Gene: Fam174a
Match Type: L24R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-CTCCC TTCTTCCCCAGATCCAT TTCACCTCCATTTCCTCTTCATAA ACCAGCAGACCACTAAG AGACA-3'
	3'-GAGGG AAGAAGGGGTCTAGGTA AAGTGGAGGTAAAGGAGAAGTATT TGGTCGTCTGGTGATTC TCTGT-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 009-094128198
Forward Primer: 5'-GTGACACTGGGAAAATCAGTAGGC-3' T_m: 59.038
Reverse Primer: 5'-GGTGTGTCTCTGGCTCTTTTGC-3' T_m: 59.258
Expected PCR Product Length: 378
Expected CelI Cleavage Product Sizes: 247, 131
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr9:94128414-94128471

Rank: 70	Homology Score: 8
	Genomic Region: Exon	Closest Gene: Pik3r5
Match Type: R15L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-TGACT TTCAGTGGTGCTCTGCC     CTAGTGCACCTAGGT      ATGAGGCTGGACAGCAA AGTGC-3'
	3'-ACTGA AAGTCACCACGAGACGG     GATCACGTGGATCCA      TACTCCGACCTGTCGTT TCACG-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 010-055247764
Forward Primer: 5'-TGCTACAAGCCAGAGAAGAGCAG-3' T_m: 59.148
Reverse Primer: 5'-AGACGTCCCTGGTCCATAGC-3' T_m: 59.508
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 131, 139
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr10:55247869-55247917

Rank: 71	Homology Score: 8
	Genomic Region: Intron	Closest Gene: Bai1
Match Type: R20L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-CCATG TTCTGTGTTGTCTTTGT   TTCTTTGTTGTTAAACTGGG   ATGGGGAGGGGCAGGTA CGCTT-3'
	3'-GGTAC AAGACACAACAGAAACA   AAGAAACAACAATTTGACCC   TACCCCTCCCCGTCCAT GCGAA-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 007-112677493
Forward Primer: 5'-TAGGGCAGAGCTGATGGACC-3' T_m: 59.508
Reverse Primer: 5'-CGAGTCTCTTCTTGACTGTTTCCC-3' T_m: 59.038
Expected PCR Product Length: 274
Expected CelI Cleavage Product Sizes: 137, 137
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr7:112677601-112677654

Rank: 72	Homology Score: 8
	Genomic Region: Intron	Closest Gene: Pik3c2a
Match Type: L23R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-GGCTA TTCTAGCCCAGGTCCAG ATGGGTTTATAGTGCAGAATTCT  ACCAGACCTTCAATGAA GACCT-3'
	3'-CCGAT AAGATCGGGTCCAGGTC TACCCAAATATCACGTCTTAAGA  TGGTCTGGAAGTTACTT CTGGA-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 001-174487424
Forward Primer: 5'-GGCCTTAAATGGAATGCATGCTGG-3' T_m: 59.038
Reverse Primer: 5'-CATCCCTGGGATGAAACCTACTTG-3' T_m: 59.038
Expected PCR Product Length: 412
Expected CelI Cleavage Product Sizes: 133, 279
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr1:174487527-174487583

Rank: 73	Homology Score: 8
	Genomic Region: Intron	Closest Gene: Grm8
Match Type: L13R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-ACCCT TGCCTTCCCTGCTCCTT      CTTCTCTGACTGT       TCTAGCACAGCACTGAA AAAGA-3'
	3'-TGGGA ACGGAAGGGACGAGGAA      GAAGAGACTGACA       AGATCGTGTCGTGACTT TTTCT-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 004-054569528
Forward Primer: 5'-CTGTGAGCAAGCCAACAGATAGC-3' T_m: 59.148
Reverse Primer: 5'-CCTTGGAGTTGCTACAGGCTTTG-3' T_m: 59.148
Expected PCR Product Length: 273
Expected CelI Cleavage Product Sizes: 135, 138
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr4:54569638-54569684

Rank: 74	Homology Score: 8
	Genomic Region: Intron	Closest Gene: Map2k1
Match Type: L15R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-CAGCC TTCCTTCCCATCTACAG     AAGAAATCAGCGAAG      AGCAGAACTCCCCTGAA GTCAG-3'
	3'-GTCGG AAGGAAGGGTAGATGTC     TTCTTTAGTCGCTTC      TCGTCTTGAGGGGACTT CAGTC-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 008-068387105
Forward Primer: 5'-CCGACTAGCTTCTCCCATGAC-3' T_m: 59.378
Reverse Primer: 5'-TTACTGTCGGTGTCACAGTGCC-3' T_m: 59.258
Expected PCR Product Length: 322
Expected CelI Cleavage Product Sizes: 191, 131
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr8:68387270-68387318

Rank: 75	Homology Score: 8
	Genomic Region: Intron	Closest Gene: Cdh5
Match Type: R20L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-CTTTA TTCAGGGTTGTTATGTA   GTTACTTTATACTTAGGTAA   CTCTAGGTGGGCAGGAA GGTTT-3'
	3'-GAAAT AAGTCCCAACAATACAT   CAATGAAATATGAATCCATT   GAGATCCACCCGTCCTT CCAAA-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 019-000783634
Forward Primer: 5'-CTCCCCCAAGACACTTTGTCTTAC-3' T_m: 59.038
Reverse Primer: 5'-AAGTATGTGAGAATGCTGCCCAGG-3' T_m: 59.038
Expected PCR Product Length: 273
Expected CelI Cleavage Product Sizes: 143, 130
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr19:783748-783801

Rank: 76	Homology Score: 8
	Genomic Region: Intron	Closest Gene: Col27a1
Match Type: L24R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-CTGGC TTCCTGCCTATCTCCTG CCCTATGCCAGTCACAGGGTAGCT AGGTGAACAACACTGAG CTTTG-3'
	3'-GACCG AAGGACGGATAGAGGAC GGGATACGGTCAGTGTCCCATCGA TCCACTTGTTGTGACTC GAAAC-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 005-080218256
Forward Primer: 5'-TCTCCCTAAGAGAAGGGGTGG-3' T_m: 59.378
Reverse Primer: 5'-GTCACTGCTTACACTGAATGACCC-3' T_m: 59.038
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 134, 136
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr5:80218359-80218416

Rank: 77	Homology Score: 8
	Genomic Region: Intergenic	Closest Gene: Dut
Match Type: R24L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-TCTAT CTTAGGGTTGTTCTTGT TTCGATGAAATACCATGACCAAAA AGCAAGCTGGGGAGGAA AGGCT-3'
	3'-AGATA GAATCCCAACAAGAACA AAGCTACTTTATGGTACTGGTTTT TCGTTCGACCCCTCCTT TCCGA-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 003-112574818
Forward Primer: 5'-GACCTCTCCAAGTCCTTTAGAGC-3' T_m: 59.148
Reverse Primer: 5'-CACTCTCCATGATCTCTGCGTC-3' T_m: 59.258
Expected PCR Product Length: 282
Expected CelI Cleavage Product Sizes: 152, 130
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr3:112574939-112574996

Rank: 78	Homology Score: 8
	Genomic Region: Intergenic	Closest Gene: Lamp5
Match Type: L24R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-ATACC TTCCACCCCACCTCCTT CCCCTAGATTTCAAGAAGGGGGGA AGCAGAACAAAACCTAA CCTCG-3'
	3'-TATGG AAGGTGGGGTGGAGGAA GGGGATCTAAAGTTCTTCCCCCCT TCGTCTTGTTTTGGATT GGAGC-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 003-124119953
Forward Primer: 5'-CAGTAGCTGCCTTTGACCAGGA-3' T_m: 59.258
Reverse Primer: 5'-CAAACCAGACAATGGCTTCTGCTC-3' T_m: 59.038
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 140, 130
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr3:124120062-124120119

Rank: 79	Homology Score: 8
	Genomic Region: Intergenic	Closest Gene: Actrt1
Match Type: R14L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-ATTTA TTAATTGTTGGTCTGAT      TACCTATGTTATTA      ATGGTTCTGTTCAGGAA GTTTT-3'
	3'-TAAAT AATTAACAACCAGACTA      ATGGATACAATAAT      TACCAAGACAAGTCCTT CAAAA-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Warning! Primer pair was generated using suboptimal parameters. 
Primer Index: 00X-131557987
Forward Primer: 5'-TTTTGGATGTCAGCTATAGGGTTGGAGAAAATCTT-3' T_m: 59.578
Reverse Primer: 5'-CAACAAATGATGCTAATCTAACTGGATGTCTACATG-3' T_m: 59.498
Expected PCR Product Length: 337
Expected CelI Cleavage Product Sizes: 131, 206
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chrX:131558092-131558139

Rank: 80	Homology Score: 8
	Genomic Region: Intergenic	Closest Gene: Amigo2
Match Type: R18L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-GGTTC TTCAGTGATGTACTGCA    TCAGGATGTGTAAGACAG    ATGAACCTGGGCTGGAG AGATG-3'
	3'-CCAAG AAGTCACTACATGACGT    AGTCCTACACATTCTGTC    TACTTGGACCCGACCTC TCTAC-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 007-135956125
Forward Primer: 5'-CACTCCTACGTAGGTATCTTGGG-3' T_m: 59.148
Reverse Primer: 5'-CTCACCAGAAGAGGGCATCAAATC-3' T_m: 59.038
Expected PCR Product Length: 280
Expected CelI Cleavage Product Sizes: 137, 143
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr7:135956234-135956285

Rank: 81	Homology Score: 8
	Genomic Region: Intergenic	Closest Gene: Prdm2
Match Type: R19L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-TACCT TTCAGGGTTGTTCTAAA   AACTAACTGGAGAACACAA    ATGGCGCCTGGCATGAA GTAAC-3'
	3'-ATGGA AAGTCCCAACAAGATTT   TTGATTGACCTCTTGTGTT    TACCGCGGACCGTACTT CATTG-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 005-161756246
Forward Primer: 5'-CTCTAGGGGCTAAGTATCTCAGCT-3' T_m: 59.038
Reverse Primer: 5'-GGTGGCAAAATCTAAACCCTGTGG-3' T_m: 59.038
Expected PCR Product Length: 280
Expected CelI Cleavage Product Sizes: 130, 150
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr5:161756348-161756400

Rank: 82	Homology Score: 8
	Genomic Region: Intergenic	Closest Gene: Far1
Match Type: R13L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-CTGTC TTAATTGTTGTTGTAGT      ACGGAGACTAACC       CTGAATGTGGGCAGGAA TATTC-3'
	3'-GACAG AATTAACAACAACATCA      TGCCTCTGATTGG       GACTTACACCCGTCCTT ATAAG-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 001-171517384
Forward Primer: 5'-CACCTTGTGTTTCCAGAACTCTGC-3' T_m: 59.038
Reverse Primer: 5'-CTAGTCCTATCACATCCACGGTC-3' T_m: 59.148
Expected PCR Product Length: 292
Expected CelI Cleavage Product Sizes: 157, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr1:171517516-171517562

Rank: 83	Homology Score: 8
	Genomic Region: Intergenic	Closest Gene: Sfrp2
Match Type: L13R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-TCATG TACCAGCCCAGCTGCAG      TCCAGCTTCTATG       TCCAGCACTGCACTGAA ACTGT-3'
	3'-AGTAC ATGGTCGGGTCGACGTC      AGGTCGAAGATAC       AGGTCGTGACGTGACTT TGACA-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 002-175409656
Forward Primer: 5'-CATCGGGCAGGGTTTCAAAGC-3' T_m: 59.378
Reverse Primer: 5'-GCCCAAGCTGACATTTGAGAGAC-3' T_m: 59.148
Expected PCR Product Length: 303
Expected CelI Cleavage Product Sizes: 159, 144
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr2:175409790-175409836

Rank: 84	Homology Score: 8
	Genomic Region: Intergenic	Closest Gene: Rgs10
Match Type: L22R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-AATTT TCTCTGCCCACCTCCGT  TGAAAGCATCCCCCCAAGGCTG  ATCACAAATACACTGAA GCTAA-3'
	3'-TTAAA AGAGACGGGTGGAGGCA  ACTTTCGTAGGGGGGTTCCGAC  TAGTGTTTATGTGACTT CGATT-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 001-187665749
Forward Primer: 5'-GGAGGGAAGGAGGGAAGGAA-3' T_m: 59.508
Reverse Primer: 5'-ACACCAGTCTTGCTGAGGGC-3' T_m: 59.508
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 132, 138
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr1:187665851-187665906

Rank: 85	Homology Score: 8
	Genomic Region: Intergenic	Closest Gene: Pik3ap1
Match Type: R16L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-GGCTT TTTTGTGTTGTACTAGT     TCATATCTGCAAACCA     CTGGAGCACAGCAGGAA ACAGA-3'
	3'-CCGAA AAAACACAACATGATCA     AGTATAGACGTTTGGT     GACCTCGTGTCGTCCTT TGTCT-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 001-246639403
Forward Primer: 5'-GCCCATGCGAATGGGTGGAA-3' T_m: 59.508
Reverse Primer: 5'-TCAAAAGCAGCCTCCTGATGTGTC-3' T_m: 59.038
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 134, 136
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr1:246639510-246639559

Rank: 86	Homology Score: 8
	Genomic Region: Intergenic	Closest Gene: Mir100
Match Type: L12R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-AGTAC TTCAAGACCAGCTCCTT       TGAATCGTTCTC       CCCAGAACAAGGCTGCA CCTGA-3'
	3'-TCATG AAGTTCTGGTCGAGGAA       ACTTAGCAAGAG       GGGTCTTGTTCCGACGT GGACT-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 008-044521883
Forward Primer: 5'-CAGAGGACCTGTGAGAGACAGT-3' T_m: 59.258
Reverse Primer: 5'-GAAGCAAATGTGAGAGGAAAAGCCTTGATG-3' T_m: 60.058
Expected PCR Product Length: 439
Expected CelI Cleavage Product Sizes: 308, 131
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr8:44522166-44522211

Rank: 87	Homology Score: 8
	Genomic Region: Intergenic	Closest Gene: Epha7
Match Type: R18L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-AAAAG TTCAGTGTGCTCCTGGG    TATATGGTTGGCAGTACA    ATGGAGCTCTGCAGACA GTATG-3'
	3'-TTTTC AAGTCACACGAGGACCC    ATATACCAACCGTCATGT    TACCTCGAGACGTCTGT CATAC-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 005-045632950
Forward Primer: 5'-CCAGAGACTGCCACACTTGGA-3' T_m: 59.378
Reverse Primer: 5'-CCTGCCATCTCTCTGCATATCTTC-3' T_m: 59.038
Expected PCR Product Length: 322
Expected CelI Cleavage Product Sizes: 172, 150
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr5:45633094-45633145

Rank: 88	Homology Score: 8
	Genomic Region: Intergenic	Closest Gene: Stk17b
Match Type: R21L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-TGGCA TTCCGTTTGGTCCTGGT  TACTCCTGCTGACTCATTAGA   ATTGAGCTGGCTGGGAA GGTAA-3'
	3'-ACCGT AAGGCAAACCAGGACCA  ATGAGGACGACTGAGTAATCT   TAACTCGACCGACCCTT CCATT-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 009-052013154
Forward Primer: 5'-GAGGCTGAGCTTGGCTTGCTT-3' T_m: 59.378
Reverse Primer: 5'-GCAAGGTCACATAGTGTCACTTGG-3' T_m: 59.038
Expected PCR Product Length: 311
Expected CelI Cleavage Product Sizes: 157, 154
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr9:52013282-52013336

Rank: 89	Homology Score: 8
	Genomic Region: Intergenic	Closest Gene: Tcf12
Match Type: L24R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-TGGAG TGCCTGCCCTGCTCTAG ATCAGTAAGGCTCTAGGATCAGTC TCCAGCACCACACAGAA CTTGA-3'
	3'-ACCTC ACGGACGGGACGAGATC TAGTCATTCCGAGATCCTAGTCAG AGGTCGTGGTGTGTCTT GAACT-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. Warning! The sequence of this region of the genome is poorly mapped. 
Primer Index: 008-076531196
Forward Primer: 5'-GGCTAGAAGAGGAGATATGGGG-3' T_m: 59.258
Reverse Primer: 5'-CCTAGGCTGACCTTGAACTCATTG-3' T_m: 59.038
Expected PCR Product Length: 353
Expected CelI Cleavage Product Sizes: 217, 136
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr8:76531382-76531439

Rank: 90	Homology Score: 8
	Genomic Region: Intergenic	Closest Gene: Clec14a
Match Type: L17R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-AGGTC TTGCTGCCCATCTTCAA    TAAGCCAAGAGAAAAGA     ACCAGCTTAACAGTGAA AAGTA-3'
	3'-TCCAG AACGACGGGTAGAAGTT    ATTCGGTTCTCTTTTCT     TGGTCGAATTGTCACTT TTCAT-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 006-079162173
Forward Primer: 5'-ACACAGAAGGCTCTCCAGAGAC-3' T_m: 59.258
Reverse Primer: 5'-ACTTTGATCTCACTTCAGGTCCCC-3' T_m: 59.038
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 138, 132
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr6:79162284-79162334

Rank: 91	Homology Score: 8
	Genomic Region: Intergenic	Closest Gene: Rps29
Match Type: L16R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-GCACA TTCCTCCACATTTCCAT     CTCGAGGAAAGATTGG     ATCAGATCAAGAGTGAA ACTCA-3'
	3'-CGTGT AAGGAGGTGTAAAGGTA     GAGCTCCTTTCTAACC     TAGTCTAGTTCTCACTT TGAGT-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 006-090557385
Forward Primer: 5'-GTCCCCACAGGCATATGATCG-3' T_m: 59.378
Reverse Primer: 5'-GTGCTTCGGGCAGTAGAGCT-3' T_m: 59.508
Expected PCR Product Length: 348
Expected CelI Cleavage Product Sizes: 217, 131
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr6:90557575-90557624

Rank: 92	Homology Score: 5
	Genomic Region: Exon	Closest Gene: Hsd17b6
Match Type: R14L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-ACTTC TTCAGTTCTCGTCTGGT      GGTGTTGCAAACTG      AAGGAGCAGAGGAGGGA GGTCA-3'
	3'-TGAAG AAGTCAAGAGCAGACCA      CCACAACGTTTGAC      TTCCTCGTCTCCTCCCT CCAGT-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 007-001118581
Forward Primer: 5'-GAAGAAAAGTCTGGCATCCCAGC-3' T_m: 59.148
Reverse Primer: 5'-GCCTGGACTCTTGGTCTTCCT-3' T_m: 59.378
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 135, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr7:1118690-1118737

Rank: 93	Homology Score: 5
	Genomic Region: Exon	Closest Gene: LOC686841
Match Type: R18L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-TGGCC CTCCATGTTGTTCGGGT    GGGTCCTTGTCAAAGTGG    TTGTACCTGGGCAGGGG GGTAA-3'
	3'-ACCGG GAGGTACAACAAGCCCA    CCCAGGAACAGTTTCACC    AACATGGACCCGTCCCC CCATT-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Acceptable Primer Pair Not Found!
Left Primers Found: 0
Right Primers Found: 270
Primers may not have been generated due to repetitive genomic sequence.
Primer pairs may not have been generated since primer dimers would form or product sizes are incompatible with specified parameters.
A careful manual examination of the genomic sequence may allow design of primers suitable for amplification of desired product.
View in Genome Browser, Chromosomal Coordinates - chr7:116335856-116335907

Rank: 94	Homology Score: 5
	Genomic Region: Exon	Closest Gene: Atrn
Match Type: L24R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-CACCT TTCCTCTCCTGCTTCAT ACCCTGCAGTTCTCCTATTATCTG ACTAGAACAGCCCTCTA GGCAA-3'
	3'-GTGGA AAGGAGAGGACGAAGTA TGGGACGTCAAGAGGATAATAGAC TGATCTTGTCGGGAGAT CCGTT-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 003-118698225
Forward Primer: 5'-GTTAAGAGGGGTGAGCAGTCC-3' T_m: 59.378
Reverse Primer: 5'-CTCAGAGAAAGGCTCCCTCTTCA-3' T_m: 59.148
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 137, 133
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr3:118698331-118698388

Rank: 95	Homology Score: 5
	Genomic Region: Exon	Closest Gene: Hsd17b6
Match Type: R14L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-ACTTC TTCAGTTCTCGTCTGGT      GGTGTTGCAAACTG      AAGGAGCAGAGGAGGGA GGTCA-3'
	3'-TGAAG AAGTCAAGAGCAGACCA      CCACAACGTTTGAC      TTCCTCGTCTCCTCCCT CCAGT-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 007-001268176
Forward Primer: 5'-GAAGAAAAGTCTGGCATCCCAGC-3' T_m: 59.148
Reverse Primer: 5'-GCCTGGACTCTTGGTCTTCCT-3' T_m: 59.378
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 135, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr7:1268285-1268332

Rank: 96	Homology Score: 5
	Genomic Region: Exon	Closest Gene: Cxcl10
Match Type: L13R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-CTACA TTCGAGCCCAGCCACAT      CCCGAGCCAACCT       TCCAGAAGCACCATGAA CCCAA-3'
	3'-GATGT AAGCTCGGGTCGGTGTA      GGGCTCGGTTGGA       AGGTCTTCGTGGTACTT GGGTT-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 014-017265922
Forward Primer: 5'-CAGGGAGGGAAACTCTGTGC-3' T_m: 59.508
Reverse Primer: 5'-GAGCTCAGTCCTCTTTCTGACTCT-3' T_m: 59.038
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 139, 131
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr14:17266036-17266082

Rank: 97	Homology Score: 5
	Genomic Region: Exon	Closest Gene: Ube2q1
Match Type: R16L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-TCTTC TACAGGTATGTTCTGGG     CGGAGGTGCCATCTGC     ATGGAGCTGCTCACCAA GCAGG-3'
	3'-AGAAG ATGTCCATACAAGACCC     GCCTCCACGGTAGACG     TACCTCGACGAGTGGTT CGTCC-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 002-181986166
Forward Primer: 5'-AGTCCTCTCTGGAGGGTGAGT-3' T_m: 59.378
Reverse Primer: 5'-GAAAGGGCCATATTATGGCTGCAG-3' T_m: 59.038
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 135, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr2:181986274-181986323

Rank: 98	Homology Score: 5
	Genomic Region: Exon	Closest Gene: Birc6
Match Type: L23R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-AGCAA TTCTAGCAGAGCTCGAT AGAGTGGCACATGTCTTGCCATG  TCCAGAACTACACAGAG AAAAG-3'
	3'-TCGTT AAGATCGTCTCGAGCTA TCTCACCGTGTACAGAACGGTAC  AGGTCTTGATGTGTCTC TTTTC-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 006-020685813
Forward Primer: 5'-GTGTAGGTATCGACACAGGTCTTC-3' T_m: 59.038
Reverse Primer: 5'-CTGCCAGAGTGGCCTACTTAATC-3' T_m: 59.148
Expected PCR Product Length: 423
Expected CelI Cleavage Product Sizes: 185, 238
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr6:20685968-20686024

Rank: 99	Homology Score: 5
	Genomic Region: Exon	Closest Gene: Rap1gds1
Match Type: L14R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-CACCG TGCTTTCCCAGCACCAA      AAAATAAACCAGAA      ACCAAAACAACAACAAA AACAA-3'
	3'-GTGGC ACGAAAGGGTCGTGGTT      TTTTATTTGGTCTT      TGGTTTTGTTGTTGTTT TTGTT-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 002-236523497
Forward Primer: 5'-GGGAGAAATCGCAGGGAAAGAATG-3' T_m: 59.038
Reverse Primer: 5'-CTCCTCACACGCAGTAACCTACA-3' T_m: 59.148
Expected PCR Product Length: 287
Expected CelI Cleavage Product Sizes: 141, 146
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr2:236523612-236523659

Rank: 100	Homology Score: 5
	Genomic Region: Exon	Closest Gene: Man2b1
Match Type: R14L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-TAGGC TGCAGTGTGATTGAGGT      AGGGTCCAACCGGG      AAGGAGCAGGGTAGGAG ATGGG-3'
	3'-ATCCG ACGTCACACTAACTCCA      TCCCAGGTTGGCCC      TTCCTCGTCCCATCCTC TACCC-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 019-024711411
Forward Primer: 5'-TGAGTGGTTCCAGCAAGAGAAGG-3' T_m: 59.148
Reverse Primer: 5'-GGTCTTCAGGCATAGAGAAGTGG-3' T_m: 59.148
Expected PCR Product Length: 275
Expected CelI Cleavage Product Sizes: 131, 144
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr19:24711516-24711563

Plate format Oligo Order Form
Well Name Sequence 5' mods Internal Notes
Primer Plate #1
A1 006-138443970F GGAATCGGCACATGCAGATCTTTG Tesson_IgM_Ranking-Homology_5TC	Primer pair generated using optimal parameters. 
A2 006-138443970R TCTGGTCCTCAGAGAGTCAGTC Tesson_IgM_Ranking-Homology_5TC	Primer pair generated using optimal parameters. 
B1 001-252428669F GTTGTGTTTCAGGACAGCACTACC Tesson_IgM_Ranking-Homology_5TC	Primer pair generated using optimal parameters. 
B2 001-252428669R GTTGGAGAGGCCACATTCTTAACC Tesson_IgM_Ranking-Homology_5TC	Primer pair generated using optimal parameters. 
C1 020-016460972F GTCAAGGACGAAGTCATCTGGAG Tesson_IgM_Ranking-Homology_5TC	Primer pair generated using optimal parameters. 
C2 020-016460972R GACAAGCTGACTGCTGCTAGAG Tesson_IgM_Ranking-Homology_5TC	Primer pair generated using optimal parameters. 
D1 009-007190270F AGTTGGGCTGGTAGAAATTGCTGG Tesson_IgM_Ranking-Homology_5TC	Primer pair generated using optimal parameters. 
D2 009-007190270R CTGAGTGAAACAAACGGCTCCGA Tesson_IgM_Ranking-Homology_5TC	Primer pair generated using optimal parameters. 
E1 015-087079625F CAATCACTCCCTGCAATCCCC Tesson_IgM_Ranking-Homology_5TC	Primer pair generated using optimal parameters. 
E2 015-087079625R TGACGCCTCCACTGTCCTAG Tesson_IgM_Ranking-Homology_5TC	Primer pair generated using optimal parameters. 
F1 007-139879252F AAAGGCTCATTAGACACGCTCAGG Tesson_IgM_Ranking-Homology_5TC	Primer pair generated using optimal parameters. 
F2 007-139879252R CACGTCTCTGCAGACCCCAT Tesson_IgM_Ranking-Homology_5TC	Primer pair generated using optimal parameters. 
G1 002-159432579F GCTAAATCCCCAACCCTGCAG Tesson_IgM_Ranking-Homology_5TC	Primer pair generated using optimal parameters. 
G2 002-159432579R GTGTTTACTTGCTCAAACACCCGG Tesson_IgM_Ranking-Homology_5TC	Primer pair generated using optimal parameters. 
H1 002-204001007F AATTTATGGGCTCTGTGCCGTGC Tesson_IgM_Ranking-Homology_5TC	Primer pair generated using optimal parameters. 
H2 002-204001007R CCCAGTGCTAAGTTGAGTGTGGC Tesson_IgM_Ranking-Homology_5TC	Primer pair generated using optimal parameters. 
A3 003-100952790F GCCATGAAGAAGACATGCAGGAG Tesson_IgM_Ranking-Homology_5TC	Primer pair generated using optimal parameters. 
A4 003-100952790R GGATCACTTGAAATGTGGAGCTTCCTAC Tesson_IgM_Ranking-Homology_5TC	Primer pair generated using optimal parameters. 
B3 00X-144712285F CCAAGCGAGACAGAGATCGG Tesson_IgM_Ranking-Homology_5TC	Primer pair generated using optimal parameters. 
B4 00X-144712285R GGTACTTGGCCCTCAGAATCG Tesson_IgM_Ranking-Homology_5TC	Primer pair generated using optimal parameters. 
C3 001-189623849F TGCATTCCCACCCCCATACTG Tesson_IgM_Ranking-Homology_5TC	Primer pair generated using optimal parameters. 
C4 001-189623849R CTGAGCACATTCAGGCATCTGG Tesson_IgM_Ranking-Homology_5TC	Primer pair generated using optimal parameters. 
D3 011-031478392F GATCTGATCCCCTTTTCTGGTGTG Tesson_IgM_Ranking-Homology_5TC	Primer pair generated using optimal parameters. 
D4 011-031478392R CTTCTTTAGCACGTCGCCCC Tesson_IgM_Ranking-Homology_5TC	Primer pair generated using optimal parameters. 
E3 006-046986724F CTTCTGGCTTAGTTTCCGTCTGTC Tesson_IgM_Ranking-Homology_5TC	Primer pair generated using optimal parameters. 
E4 006-046986724R CTCAGCTTGCCCAAGGGTTATAG Tesson_IgM_Ranking-Homology_5TC	Primer pair generated using optimal parameters. 
F3 014-008802083F TGCCAGGCTCCTGTCTGCAT Tesson_IgM_Ranking-Homology_5TC	Primer pair generated using optimal parameters. 
F4 014-008802083R GCTACCAGGACTCAATGGGG Tesson_IgM_Ranking-Homology_5TC	Primer pair generated using optimal parameters. 
G3 007-014531040F TCTCTGTATCAGGTGGCCCC Tesson_IgM_Ranking-Homology_5TC	Primer pair generated using optimal parameters. 
G4 007-014531040R CAAGAATCTCCCCATGATGAGGTG Tesson_IgM_Ranking-Homology_5TC	Primer pair generated using optimal parameters. 
H3 007-067524783F TGATCAGAGCCTGCCCCTTC Tesson_IgM_Ranking-Homology_5TC	Primer pair generated using optimal parameters. 
H4 007-067524783R GACAGGGTGCTTCCTCACAATAC Tesson_IgM_Ranking-Homology_5TC	Primer pair generated using optimal parameters. 
A5 020-012161079F CCGTTGCCCGAAAAGCACCT Tesson_IgM_Ranking-Homology_5TC	Primer pair generated using optimal parameters. 
A6 020-012161079R GAACCCATTTGCTCTCAGCCTTG Tesson_IgM_Ranking-Homology_5TC	Primer pair generated using optimal parameters. 
B5 001-138061468F CCGTGAAGCAGGTCACAAAGC Tesson_IgM_Ranking-Homology_5TC	Primer pair generated using optimal parameters. Warning! The sequence of this region of the genome is poorly mapped. 
B6 001-138061468R CAGCAAGGGAAGTGCTACTTGATG Tesson_IgM_Ranking-Homology_5TC	Primer pair generated using optimal parameters. Warning! The sequence of this region of the genome is poorly mapped. 
C5 010-013862379F CATAGGTGGGGCCTTATCAGAG Tesson_IgM_Ranking-Homology_5TC	Primer pair generated using optimal parameters. 
C6 010-013862379R GGTCCCTAGTCAAGGACATTGTCT Tesson_IgM_Ranking-Homology_5TC	Primer pair generated using optimal parameters. 
D5 004-141883124F AATCCGCAGTCTAAGCCAACAGAG Tesson_IgM_Ranking-Homology_5TC	Primer pair generated using optimal parameters. 
D6 004-141883124R TGCTAGGCAAGCACTCTACCAC Tesson_IgM_Ranking-Homology_5TC	Primer pair generated using optimal parameters. 
E5 003-145456877F GGCGACAGTTCCTCTGTTTTTGC Tesson_IgM_Ranking-Homology_5TC	Primer pair generated using optimal parameters. 
E6 003-145456877R CACAGTAGTGGCTGTAGGCATTTG Tesson_IgM_Ranking-Homology_5TC	Primer pair generated using optimal parameters. 
F5 012-015035708F AGCCTCCTGGTTACGTCCCAA Tesson_IgM_Ranking-Homology_5TC	Primer pair generated using optimal parameters. 
F6 012-015035708R TGACTGCTGTGTGTGGGGAG Tesson_IgM_Ranking-Homology_5TC	Primer pair generated using optimal parameters. 
G5 002-214232497F CACGTGTGTGCCTCAGTAACAG Tesson_IgM_Ranking-Homology_5TC	Primer pair generated using optimal parameters. 
G6 002-214232497R AGCAGGCCCTGGAACAGTTCT Tesson_IgM_Ranking-Homology_5TC	Primer pair generated using optimal parameters. 
H5 001-242536006F TGGAGGTTGGGAGAGTAGAGG Tesson_IgM_Ranking-Homology_5TC	Primer pair generated using optimal parameters. 
H6 001-242536006R TGAAGGAGGGAACACTCAGCAG Tesson_IgM_Ranking-Homology_5TC	Primer pair generated using optimal parameters. 
A7 001-256112389F CCTGGATAAGTCAGTAAAGGGTGG Tesson_IgM_Ranking-Homology_5TC	Primer pair generated using optimal parameters. 
A8 001-256112389R CCTTGGTGGGACCTGATACAGT Tesson_IgM_Ranking-Homology_5TC	Primer pair generated using optimal parameters. 
B7 005-049320556F CTGTGGTGAAGGTGTTACTGTGTC Tesson_IgM_Ranking-Homology_5TC	Primer pair generated using optimal parameters. 
B8 005-049320556R GAGAAGGCTGGACCGAATAGAAG Tesson_IgM_Ranking-Homology_5TC	Primer pair generated using optimal parameters. 
C7 006-075907048F GGTGTCCTCATTCACCTCTGACAA Tesson_IgM_Ranking-Homology_5TC	Primer pair generated using optimal parameters. 
C8 006-075907048R GTGTTTGGAACATCCTCTGCGAC Tesson_IgM_Ranking-Homology_5TC	Primer pair generated using optimal parameters. 
D7 014-082098705F AGCCAAGGAAGAAACCTGTGCAGC Tesson_IgM_Ranking-Homology_5TC	Primer pair generated using optimal parameters. 
D8 014-082098705R CTGTGGATGGCTAGAGTGTACTCA Tesson_IgM_Ranking-Homology_5TC	Primer pair generated using optimal parameters. 
E7 014-085768096F GGGAATGGTTAGCCCCATCTC Tesson_IgM_Ranking-Homology_5TC	Primer pair generated using optimal parameters. 
E8 014-085768096R CATGAGGAAACAGTTCAGGAGGG Tesson_IgM_Ranking-Homology_5TC	Primer pair generated using optimal parameters. 
F7 004-100499585F GGCATAGCCTCTCAGGAAACAG Tesson_IgM_Ranking-Homology_5TC	Primer pair generated using optimal parameters. 
F8 004-100499585R CTTCCAGGACAGAACAGGAAAGAC Tesson_IgM_Ranking-Homology_5TC	Primer pair generated using optimal parameters. 
G7 002-106965669F ATAGTGGCCAGTGCCTGGAC Tesson_IgM_Ranking-Homology_5TC	Primer pair generated using optimal parameters. 
G8 002-106965669R CCAGGAGAAAAGGCCATCTTCCA Tesson_IgM_Ranking-Homology_5TC	Primer pair generated using optimal parameters. 
H7 005-113632907F GGAGGAATCTCTAGAAAGTCCCAG Tesson_IgM_Ranking-Homology_5TC	Primer pair generated using optimal parameters. 
H8 005-113632907R GGCTCCTATCTGCAGGTCCA Tesson_IgM_Ranking-Homology_5TC	Primer pair generated using optimal parameters. 
A9 00X-117110415F CTTTGCCCACAGAATAGCAGCC Tesson_IgM_Ranking-Homology_5TC	Primer pair generated using optimal parameters. 
A10 00X-117110415R CTAGCCAAGCGCGACAAAGTTC Tesson_IgM_Ranking-Homology_5TC	Primer pair generated using optimal parameters. 
B9 006-122127331F GATATTGGGAGACCCCCTAACTCA Tesson_IgM_Ranking-Homology_5TC	Primer pair generated using optimal parameters. 
B10 006-122127331R GGGCATGACCCAACTACAATTACC Tesson_IgM_Ranking-Homology_5TC	Primer pair generated using optimal parameters. 
C9 005-123636672F CTCTGAGTGACACGAATTAGTGGG Tesson_IgM_Ranking-Homology_5TC	Primer pair generated using optimal parameters. 
C10 005-123636672R GGAGTGTGTCACTAAGGCTGG Tesson_IgM_Ranking-Homology_5TC	Primer pair generated using optimal parameters. 
D9 001-127719260F CCAACATTCTCCCAAGAGATTCTGGAAAC Tesson_IgM_Ranking-Homology_5TC	Primer pair generated using optimal parameters. 
D10 001-127719260R GTTCCAGGTCAACCAGGACTAC Tesson_IgM_Ranking-Homology_5TC	Primer pair generated using optimal parameters. 
E9 00X-133545933F CGCTCTTCAAGGTATTTTGCCCAC Tesson_IgM_Ranking-Homology_5TC	Primer pair generated using optimal parameters. 
E10 00X-133545933R CTAGCCAAACACGACAAAGATCGG Tesson_IgM_Ranking-Homology_5TC	Primer pair generated using optimal parameters. 
F9 001-140972716F GGGACCACCTCAGCTAAGGA Tesson_IgM_Ranking-Homology_5TC	Primer pair generated using optimal parameters. 
F10 001-140972716R GTGGGGTTGTTGGTTTTCAGCTC Tesson_IgM_Ranking-Homology_5TC	Primer pair generated using optimal parameters. 
G9 00X-146103263F CTACACTCCTTTTTGTTGGGTGGG Tesson_IgM_Ranking-Homology_5TC	Primer pair generated using optimal parameters. 
G10 00X-146103263R CTCTGCTGCAGAGCCTTTTTTCC Tesson_IgM_Ranking-Homology_5TC	Primer pair generated using optimal parameters. 
H9 003-150734535F CACCAGCCAAGAACGGCGTCTTT Tesson_IgM_Ranking-Homology_5TC	Primer pair generated using optimal parameters. 
H10 003-150734535R CACCTCTGCACCTCCTTCTAAC Tesson_IgM_Ranking-Homology_5TC	Primer pair generated using optimal parameters. 
A11 012-015478690F CCACTCAAGACAAGCAGAAAGGAC Tesson_IgM_Ranking-Homology_5TC	Primer pair generated using optimal parameters. 
A12 012-015478690R CAAAACAGCAGCAGCAAAAGCTGG Tesson_IgM_Ranking-Homology_5TC	Primer pair generated using optimal parameters. 
B11 001-161161633F CTGCTGAGGAATCTCAAATGGCTG Tesson_IgM_Ranking-Homology_5TC	Primer pair generated using optimal parameters. 
B12 001-161161633R CATTTCCACCAGCAATGGAGGAG Tesson_IgM_Ranking-Homology_5TC	Primer pair generated using optimal parameters. 
C11 00X-016509931F CTACCAATGTCTATGGAGTGACCG Tesson_IgM_Ranking-Homology_5TC	Primer pair generated using optimal parameters. 
C12 00X-016509931R GCCTAGAGTATCCCAAATGTGCAC Tesson_IgM_Ranking-Homology_5TC	Primer pair generated using optimal parameters. 
D11 001-001765937F TTCTAGCCAAGAGGTCCAAAGGCC Tesson_IgM_Ranking-Homology_5TC	Primer pair generated using optimal parameters. 
D12 001-001765937R TGCATGGCCTCTGCATCAGC Tesson_IgM_Ranking-Homology_5TC	Primer pair generated using optimal parameters. 
E11 009-019047855F GTGGCTTTGGTCCTCATCTGTC Tesson_IgM_Ranking-Homology_5TC	Primer pair generated using optimal parameters. 
E12 009-019047855R CCTCTTTCGGAGATAGGCATGTTC Tesson_IgM_Ranking-Homology_5TC	Primer pair generated using optimal parameters. 
F11 0Un-021787610F CCTGGGATGTGGTGTAAGTTGG Tesson_IgM_Ranking-Homology_5TC	Primer pair generated using optimal parameters. 
F12 0Un-021787610R GAGGAGCCAAATCTGGACCATTAG Tesson_IgM_Ranking-Homology_5TC	Primer pair generated using optimal parameters. 
G11 013-002441765F CTGTCTCTGTCTCTCTTAGTTCCC Tesson_IgM_Ranking-Homology_5TC	Primer pair generated using optimal parameters. 
G12 013-002441765R CCCTTTTTGCCTTTCCATGCAGTG Tesson_IgM_Ranking-Homology_5TC	Primer pair generated using optimal parameters. 
H11 007-025308852F CCAGTCTGATGGTTATACATACAGTGCAGT Tesson_IgM_Ranking-Homology_5TC	Primer pair generated using optimal parameters. 
H12 007-025308852R GGTTTTCAACCTGAGTGGGAGAAG Tesson_IgM_Ranking-Homology_5TC	Primer pair generated using optimal parameters. 
Primer Plate #2
A1 00X-027254372F CCATTATCGCTAGCCTAGCTTACG Tesson_IgM_Ranking-Homology_5TC	Primer pair generated using optimal parameters. 
A2 00X-027254372R GACTGGGAATAAGTGGTGTGGTAC Tesson_IgM_Ranking-Homology_5TC	Primer pair generated using optimal parameters. 
B1 011-028431745F GCTCAGAACAGCTAATGTGAGTGC Tesson_IgM_Ranking-Homology_5TC	Primer pair generated using optimal parameters. 
B2 011-028431745R CAATGACTGTCCAGAACTGAGGC Tesson_IgM_Ranking-Homology_5TC	Primer pair generated using optimal parameters. 
C1 012-031781347F CGCGTAATACGCCTTTTGTTGACC Tesson_IgM_Ranking-Homology_5TC	Primer pair generated using optimal parameters. 
C2 012-031781347R GAGCAGTGAACAGCATTAGCTTCC Tesson_IgM_Ranking-Homology_5TC	Primer pair generated using optimal parameters. 
D1 dom-003376644F CCTGGGATGTGGTGTAAGTTGG Tesson_IgM_Ranking-Homology_5TC	Primer pair generated using optimal parameters. 
D2 dom-003376644R GAGGAGCCAAATCTGGACCATTAG Tesson_IgM_Ranking-Homology_5TC	Primer pair generated using optimal parameters. 
E1 004-003843423F GGTGACCAATACCAACCATCAGG Tesson_IgM_Ranking-Homology_5TC	Primer pair generated using optimal parameters. 
E2 004-003843423R CCTCCTTTCAGGAAGGCTCTTTCT Tesson_IgM_Ranking-Homology_5TC	Primer pair generated using optimal parameters. 
F1 0Un-038608458F CCTGGGATGTGGTGTAAGTTGG Tesson_IgM_Ranking-Homology_5TC	Primer pair generated using optimal parameters. Warning! The sequence of this region of the genome is poorly mapped. 
F2 0Un-038608458R GAGACAACATACCCTGCTCTCAC Tesson_IgM_Ranking-Homology_5TC	Primer pair generated using optimal parameters. Warning! The sequence of this region of the genome is poorly mapped. 
G1 014-039438747F GCTCCATAAACTGGGCCTGAGA Tesson_IgM_Ranking-Homology_5TC	Primer pair generated using optimal parameters. 
G2 014-039438747R GCTAGCCTCTACCTCTTCCTG Tesson_IgM_Ranking-Homology_5TC	Primer pair generated using optimal parameters. 
H1 017-041218534F CCAGGCCATGATTATTCTGCAGG Tesson_IgM_Ranking-Homology_5TC	Primer pair generated using optimal parameters. 
H2 017-041218534R TCCCAAGTCACCTGCAAGCC Tesson_IgM_Ranking-Homology_5TC	Primer pair generated using optimal parameters. 
A3 014-042588777F GAGACTACAGATGCCAAAAGTTTCCATACC Tesson_IgM_Ranking-Homology_5TC	Primer pair generated using optimal parameters. 
A4 014-042588777R ACACACACACACACACACACACAC Tesson_IgM_Ranking-Homology_5TC	Primer pair generated using optimal parameters. 
B3 009-004673112F AATCCACCGAGCCTAGTAACTGC Tesson_IgM_Ranking-Homology_5TC	Primer pair generated using optimal parameters. 
B4 009-004673112R TACACTGCGCTCACACAGCAAG Tesson_IgM_Ranking-Homology_5TC	Primer pair generated using optimal parameters. 
C3 0Un-051334472F CCTGGGATGTGGTGTAAGTTGG Tesson_IgM_Ranking-Homology_5TC	Primer pair generated using optimal parameters. 
C4 0Un-051334472R GAGGAGCCAAATCTGGACCATTAG Tesson_IgM_Ranking-Homology_5TC	Primer pair generated using optimal parameters. 
D3 0Un-051940753F CAAACAGAGCCAGGTGTGGTG Tesson_IgM_Ranking-Homology_5TC	Primer pair generated using optimal parameters. 
D4 0Un-051940753R GCCCTCTGTGTCATGCCTTATTAC Tesson_IgM_Ranking-Homology_5TC	Primer pair generated using optimal parameters. 
E3 001-053325789F ACATGGCTTCCATACCGAGGC Tesson_IgM_Ranking-Homology_5TC	Primer pair generated using optimal parameters. 
E4 001-053325789R GAAGGGGTTCTTTGCGCCAAATAG Tesson_IgM_Ranking-Homology_5TC	Primer pair generated using optimal parameters. 
F3 019-057896612F CCCAGTGCTGGGGTTACAAACA Tesson_IgM_Ranking-Homology_5TC	Primer pair generated using optimal parameters. 
F4 019-057896612R TCTTAGCCAGGAATGAGGCTGC Tesson_IgM_Ranking-Homology_5TC	Primer pair generated using optimal parameters. 
G3 003-060396362F CCACTGGGAGTGTATTCGGTATG Tesson_IgM_Ranking-Homology_5TC	Primer pair generated using optimal parameters. 
G4 003-060396362R GTAAACAGGCCTCTACAGAACGG Tesson_IgM_Ranking-Homology_5TC	Primer pair generated using optimal parameters. 
H3 0Un-061148009F CCTGGGATGTGGTGTAAGTTGG Tesson_IgM_Ranking-Homology_5TC	Primer pair generated using optimal parameters. 
H4 0Un-061148009R GAGACAACATACCCTGCTCTCAC Tesson_IgM_Ranking-Homology_5TC	Primer pair generated using optimal parameters. 
A5 009-065106893F CCTCTGTTGGATGTGGAGTTGG Tesson_IgM_Ranking-Homology_5TC	Primer pair generated using optimal parameters. 
A6 009-065106893R CGCACACCTATGGACATCTGAG Tesson_IgM_Ranking-Homology_5TC	Primer pair generated using optimal parameters. 
B5 0Un-075352159F CTTGAGAGACTGTGGTGAATGAGG Tesson_IgM_Ranking-Homology_5TC	Primer pair generated using optimal parameters. 
B6 0Un-075352159R GGAGGAAAAAGGGGACTGTGTTCA Tesson_IgM_Ranking-Homology_5TC	Primer pair generated using optimal parameters. 
C5 004-092024652F CTTGAGAGACTGTGGTGAATGAGG Tesson_IgM_Ranking-Homology_5TC	Primer pair generated using optimal parameters. 
C6 004-092024652R GGAGGAAAAAGGGGACTGTGTTCA Tesson_IgM_Ranking-Homology_5TC	Primer pair generated using optimal parameters. 
D5 00X-093585607F CCAAGCGCGACAAAGATCGG Tesson_IgM_Ranking-Homology_5TC	Primer pair generated using optimal parameters. 
D6 00X-093585607R GGTACTTTGCCCACAGAATAGCAG Tesson_IgM_Ranking-Homology_5TC	Primer pair generated using optimal parameters. 
E5 009-094128198F GTGACACTGGGAAAATCAGTAGGC Tesson_IgM_Ranking-Homology_5TC	Primer pair generated using optimal parameters. 
E6 009-094128198R GGTGTGTCTCTGGCTCTTTTGC Tesson_IgM_Ranking-Homology_5TC	Primer pair generated using optimal parameters. 
F5 010-055247764F TGCTACAAGCCAGAGAAGAGCAG Tesson_IgM_Ranking-Homology_5TC	Primer pair generated using optimal parameters. 
F6 010-055247764R AGACGTCCCTGGTCCATAGC Tesson_IgM_Ranking-Homology_5TC	Primer pair generated using optimal parameters. 
G5 007-112677493F TAGGGCAGAGCTGATGGACC Tesson_IgM_Ranking-Homology_5TC	Primer pair generated using optimal parameters. 
G6 007-112677493R CGAGTCTCTTCTTGACTGTTTCCC Tesson_IgM_Ranking-Homology_5TC	Primer pair generated using optimal parameters. 
H5 001-174487424F GGCCTTAAATGGAATGCATGCTGG Tesson_IgM_Ranking-Homology_5TC	Primer pair generated using optimal parameters. 
H6 001-174487424R CATCCCTGGGATGAAACCTACTTG Tesson_IgM_Ranking-Homology_5TC	Primer pair generated using optimal parameters. 
A7 004-054569528F CTGTGAGCAAGCCAACAGATAGC Tesson_IgM_Ranking-Homology_5TC	Primer pair generated using optimal parameters. 
A8 004-054569528R CCTTGGAGTTGCTACAGGCTTTG Tesson_IgM_Ranking-Homology_5TC	Primer pair generated using optimal parameters. 
B7 008-068387105F CCGACTAGCTTCTCCCATGAC Tesson_IgM_Ranking-Homology_5TC	Primer pair generated using optimal parameters. 
B8 008-068387105R TTACTGTCGGTGTCACAGTGCC Tesson_IgM_Ranking-Homology_5TC	Primer pair generated using optimal parameters. 
C7 019-000783634F CTCCCCCAAGACACTTTGTCTTAC Tesson_IgM_Ranking-Homology_5TC	Primer pair generated using optimal parameters. 
C8 019-000783634R AAGTATGTGAGAATGCTGCCCAGG Tesson_IgM_Ranking-Homology_5TC	Primer pair generated using optimal parameters. 
D7 005-080218256F TCTCCCTAAGAGAAGGGGTGG Tesson_IgM_Ranking-Homology_5TC	Primer pair generated using optimal parameters. 
D8 005-080218256R GTCACTGCTTACACTGAATGACCC Tesson_IgM_Ranking-Homology_5TC	Primer pair generated using optimal parameters. 
E7 003-112574818F GACCTCTCCAAGTCCTTTAGAGC Tesson_IgM_Ranking-Homology_5TC	Primer pair generated using optimal parameters. 
E8 003-112574818R CACTCTCCATGATCTCTGCGTC Tesson_IgM_Ranking-Homology_5TC	Primer pair generated using optimal parameters. 
F7 003-124119953F CAGTAGCTGCCTTTGACCAGGA Tesson_IgM_Ranking-Homology_5TC	Primer pair generated using optimal parameters. 
F8 003-124119953R CAAACCAGACAATGGCTTCTGCTC Tesson_IgM_Ranking-Homology_5TC	Primer pair generated using optimal parameters. 
G7 00X-131557987F TTTTGGATGTCAGCTATAGGGTTGGAGAAAATCTT Tesson_IgM_Ranking-Homology_5TC	Warning! Primer pair was generated using suboptimal parameters. 
G8 00X-131557987R CAACAAATGATGCTAATCTAACTGGATGTCTACATG Tesson_IgM_Ranking-Homology_5TC	Warning! Primer pair was generated using suboptimal parameters. 
H7 007-135956125F CACTCCTACGTAGGTATCTTGGG Tesson_IgM_Ranking-Homology_5TC	Primer pair generated using optimal parameters. 
H8 007-135956125R CTCACCAGAAGAGGGCATCAAATC Tesson_IgM_Ranking-Homology_5TC	Primer pair generated using optimal parameters. 
A9 005-161756246F CTCTAGGGGCTAAGTATCTCAGCT Tesson_IgM_Ranking-Homology_5TC	Primer pair generated using optimal parameters. 
A10 005-161756246R GGTGGCAAAATCTAAACCCTGTGG Tesson_IgM_Ranking-Homology_5TC	Primer pair generated using optimal parameters. 
B9 001-171517384F CACCTTGTGTTTCCAGAACTCTGC Tesson_IgM_Ranking-Homology_5TC	Primer pair generated using optimal parameters. 
B10 001-171517384R CTAGTCCTATCACATCCACGGTC Tesson_IgM_Ranking-Homology_5TC	Primer pair generated using optimal parameters. 
C9 002-175409656F CATCGGGCAGGGTTTCAAAGC Tesson_IgM_Ranking-Homology_5TC	Primer pair generated using optimal parameters. 
C10 002-175409656R GCCCAAGCTGACATTTGAGAGAC Tesson_IgM_Ranking-Homology_5TC	Primer pair generated using optimal parameters. 
D9 001-187665749F GGAGGGAAGGAGGGAAGGAA Tesson_IgM_Ranking-Homology_5TC	Primer pair generated using optimal parameters. 
D10 001-187665749R ACACCAGTCTTGCTGAGGGC Tesson_IgM_Ranking-Homology_5TC	Primer pair generated using optimal parameters. 
E9 001-246639403F GCCCATGCGAATGGGTGGAA Tesson_IgM_Ranking-Homology_5TC	Primer pair generated using optimal parameters. 
E10 001-246639403R TCAAAAGCAGCCTCCTGATGTGTC Tesson_IgM_Ranking-Homology_5TC	Primer pair generated using optimal parameters. 
F9 008-044521883F CAGAGGACCTGTGAGAGACAGT Tesson_IgM_Ranking-Homology_5TC	Primer pair generated using optimal parameters. 
F10 008-044521883R GAAGCAAATGTGAGAGGAAAAGCCTTGATG Tesson_IgM_Ranking-Homology_5TC	Primer pair generated using optimal parameters. 
G9 005-045632950F CCAGAGACTGCCACACTTGGA Tesson_IgM_Ranking-Homology_5TC	Primer pair generated using optimal parameters. 
G10 005-045632950R CCTGCCATCTCTCTGCATATCTTC Tesson_IgM_Ranking-Homology_5TC	Primer pair generated using optimal parameters. 
H9 009-052013154F GAGGCTGAGCTTGGCTTGCTT Tesson_IgM_Ranking-Homology_5TC	Primer pair generated using optimal parameters. 
H10 009-052013154R GCAAGGTCACATAGTGTCACTTGG Tesson_IgM_Ranking-Homology_5TC	Primer pair generated using optimal parameters. 
A11 008-076531196F GGCTAGAAGAGGAGATATGGGG Tesson_IgM_Ranking-Homology_5TC	Primer pair generated using optimal parameters. Warning! The sequence of this region of the genome is poorly mapped. 
A12 008-076531196R CCTAGGCTGACCTTGAACTCATTG Tesson_IgM_Ranking-Homology_5TC	Primer pair generated using optimal parameters. Warning! The sequence of this region of the genome is poorly mapped. 
B11 006-079162173F ACACAGAAGGCTCTCCAGAGAC Tesson_IgM_Ranking-Homology_5TC	Primer pair generated using optimal parameters. 
B12 006-079162173R ACTTTGATCTCACTTCAGGTCCCC Tesson_IgM_Ranking-Homology_5TC	Primer pair generated using optimal parameters. 
C11 006-090557385F GTCCCCACAGGCATATGATCG Tesson_IgM_Ranking-Homology_5TC	Primer pair generated using optimal parameters. 
C12 006-090557385R GTGCTTCGGGCAGTAGAGCT Tesson_IgM_Ranking-Homology_5TC	Primer pair generated using optimal parameters. 
D11 007-001118581F GAAGAAAAGTCTGGCATCCCAGC Tesson_IgM_Ranking-Homology_5TC	Primer pair generated using optimal parameters. 
D12 007-001118581R GCCTGGACTCTTGGTCTTCCT Tesson_IgM_Ranking-Homology_5TC	Primer pair generated using optimal parameters. 
E11 N/A Primers Not Generated 
E12 N/A Primers Not Generated! 
F11 003-118698225F GTTAAGAGGGGTGAGCAGTCC Tesson_IgM_Ranking-Homology_5TC	Primer pair generated using optimal parameters. 
F12 003-118698225R CTCAGAGAAAGGCTCCCTCTTCA Tesson_IgM_Ranking-Homology_5TC	Primer pair generated using optimal parameters. 
G11 007-001268176F GAAGAAAAGTCTGGCATCCCAGC Tesson_IgM_Ranking-Homology_5TC	Primer pair generated using optimal parameters. 
G12 007-001268176R GCCTGGACTCTTGGTCTTCCT Tesson_IgM_Ranking-Homology_5TC	Primer pair generated using optimal parameters. 
H11 014-017265922F CAGGGAGGGAAACTCTGTGC Tesson_IgM_Ranking-Homology_5TC	Primer pair generated using optimal parameters. 
H12 014-017265922R GAGCTCAGTCCTCTTTCTGACTCT Tesson_IgM_Ranking-Homology_5TC	Primer pair generated using optimal parameters. 
Primer Plate #3
A1 002-181986166F AGTCCTCTCTGGAGGGTGAGT Tesson_IgM_Ranking-Homology_5TC	Primer pair generated using optimal parameters. 
A2 002-181986166R GAAAGGGCCATATTATGGCTGCAG Tesson_IgM_Ranking-Homology_5TC	Primer pair generated using optimal parameters. 
B1 006-020685813F GTGTAGGTATCGACACAGGTCTTC Tesson_IgM_Ranking-Homology_5TC	Primer pair generated using optimal parameters. 
B2 006-020685813R CTGCCAGAGTGGCCTACTTAATC Tesson_IgM_Ranking-Homology_5TC	Primer pair generated using optimal parameters. 
C1 002-236523497F GGGAGAAATCGCAGGGAAAGAATG Tesson_IgM_Ranking-Homology_5TC	Primer pair generated using optimal parameters. 
C2 002-236523497R CTCCTCACACGCAGTAACCTACA Tesson_IgM_Ranking-Homology_5TC	Primer pair generated using optimal parameters. 
D1 019-024711411F TGAGTGGTTCCAGCAAGAGAAGG Tesson_IgM_Ranking-Homology_5TC	Primer pair generated using optimal parameters. 
D2 019-024711411R GGTCTTCAGGCATAGAGAAGTGG Tesson_IgM_Ranking-Homology_5TC	Primer pair generated using optimal parameters.
1973 total sites were located
Mismatch Types	Genomic Regions
0 and 0: 1 3 and 3: 1 2 and 5: 2 3 and 4: 10 3 and 5: 55 4 and 4: 22 4 and 5: 408 5 and 5: 1474	Exons: 35 Promoters: 8 Introns: 485 Intergenic: 1445