PROGNOS Summary for Tesson_IgM_Ranking-Homology_5T

The rn4 genome was searched for the TALEN pair targeting:
5'- TTCCTGCCCAGCTCCAT NN..NN ACCAGAACAACACTGAA -3'

Up to 5 mismatches were allowed in each nuclease half-site.

Allowed spacing distances were: 12 13 14 15 16 17 18 19 20 21 22 23 24

Only heterodimers of the nuclease pair were included in the output

1579 total sites were located
Mismatch Types
Genomic Regions
  0 and 0: 1
  3 and 3: 1
  2 and 5: 2
  3 and 4: 6
  3 and 5: 49
  4 and 4: 19
  4 and 5: 322
  5 and 5: 1179
  Exons: 23
  Promoters: 8
  Introns: 404
  Intergenic: 1144


The top 100 sites are ranked below according to a weighted sequence homology. All sites listed contain flanking 5' thymidines.
For the full output, click here.

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Primer pairs were designed using the 400 bp of sequence on either side of the cleavage site to melt close to 60 °C

For easy visualization of cleavage on gels:
The smallest fragment will be longer than 130 bp, which is also the minimum separation distance between uncleaved and cleaved products.
The two cleavage products will be separated by a minimum size of 0 bp
To optimize assay conditions, the amplicons have a minimum length of 260 bp, a maximum length of 450 bp, and an optimal length of 270 bp.
Rank: 1	Homology Score: 72
	Genomic Region: Intergenic	Closest Gene: Adam6	Caution! This site scores very closely to the intended nuclease target. The potential for cleavage at this site is of great concern.
Match Type: R17L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-ATGAC TTCAGTGTTGTTCTGGT    AGTTCCAGGAGAAGGAA     ATGGAGCTGGGCAGGAA GTCCC-3'
	3'-TACTG AAGTCACAACAAGACCA    TCAAGGTCCTCTTCCTT     TACCTCGACCCGTCCTT CAGGG-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 006-138443970
Forward Primer: 5'-GGAATCGGCACATGCAGATCTTTG-3' Tm: 59.038
Reverse Primer: 5'-TCTGGTCCTCAGAGAGTCAGTC-3' Tm: 59.258
Expected PCR Product Length: 325
Expected CelI Cleavage Product Sizes: 194, 131
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr6:138444137-138444187

Rank: 2	Homology Score: 18
	Genomic Region: Intron	Closest Gene: Pdcd11
Match Type: R16L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-TTGGA TGCAGACTTGTTCTGGT     CCTGTTAGCAGACCTC     AGGGATCTGGGGAGGAA TTAGC-3'
	3'-AACCT ACGTCTGAACAAGACCA     GGACAATCGTCTGGAG     TCCCTAGACCCCTCCTT AATCG-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 001-252428669
Forward Primer: 5'-GTTGTGTTTCAGGACAGCACTACC-3' Tm: 59.038
Reverse Primer: 5'-GTTGGAGAGGCCACATTCTTAACC-3' Tm: 59.038
Expected PCR Product Length: 308
Expected CelI Cleavage Product Sizes: 162, 146
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr1:252428804-252428853

Rank: 3	Homology Score: 17
	Genomic Region: Intergenic	Closest Gene: RGD1559903
Match Type: R16L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-TTCCA TGCAGTGTTGTTCTGTT     GTCAGTAATGGATGCC     AGAGAGTTGGGATGGAA TGAGA-3'
	3'-AAGGT ACGTCACAACAAGACAA     CAGTCATTACCTACGG     TCTCTCAACCCTACCTT ACTCT-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 020-016460972
Forward Primer: 5'-GTCAAGGACGAAGTCATCTGGAG-3' Tm: 59.148
Reverse Primer: 5'-GACAAGCTGACTGCTGCTAGAG-3' Tm: 59.258
Expected PCR Product Length: 340
Expected CelI Cleavage Product Sizes: 144, 196
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr20:16461089-16461138

Rank: 4	Homology Score: 17
	Genomic Region: Intergenic	Closest Gene: Lrfn2
Match Type: L24R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-TCCCC TTCCTGCCCAGCTCCCA TCCCCACCCCCTTCTGAGAACTAA ACAGGAAGCACACGGAA CCTGC-3'
	3'-AGGGG AAGGACGGGTCGAGGGT AGGGGTGGGGGAAGACTCTTGATT TGTCCTTCGTGTGCCTT GGACG-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 009-007190270
Forward Primer: 5'-AGTTGGGCTGGTAGAAATTGCTGG-3' Tm: 59.038
Reverse Primer: 5'-CTGAGTGAAACAAACGGCTCCGA-3' Tm: 59.148
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 133, 137
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr9:7190372-7190429

Rank: 5	Homology Score: 13
	Genomic Region: Promoter	Closest Gene: Cln5
Match Type: R14L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-AGTGT TTTATTGTTGTTATGGT      TTGGCTGGTGGTGG      CTGCAGGTGGGCAGAAA GCACC-3'
	3'-TCACA AAATAACAACAATACCA      AACCGACCACCACC      GACGTCCACCCGTCTTT CGTGG-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 015-087079625
Forward Primer: 5'-CAATCACTCCCTGCAATCCCC-3' Tm: 59.378
Reverse Primer: 5'-TGACGCCTCCACTGTCCTAG-3' Tm: 59.508
Expected PCR Product Length: 326
Expected CelI Cleavage Product Sizes: 142, 184
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr15:87079741-87079788

Rank: 6	Homology Score: 13
	Genomic Region: Intron	Closest Gene: Ppm1l
Match Type: L12R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-ACTCT TTCCTCCCAAGCTGCTT       TTGGGCGGTTTT       ACCACAATAACAGTGAA GCAAA-3'
	3'-TGAGA AAGGAGGGTTCGACGAA       AACCCGCCAAAA       TGGTGTTATTGTCACTT CGTTT-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 002-159432579
Forward Primer: 5'-GCTAAATCCCCAACCCTGCAG-3' Tm: 59.378
Reverse Primer: 5'-GTGTTTACTTGCTCAAACACCCGG-3' Tm: 59.038
Expected PCR Product Length: 284
Expected CelI Cleavage Product Sizes: 154, 130
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr2:159432708-159432753

Rank: 7	Homology Score: 13
	Genomic Region: Intron	Closest Gene: Sars
Match Type: L22R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-GTAAG TACCTCCCCAGCTCAAT  TCCACTCACACAACCACAGATA  ACCAGGCCAAAACAGAA GTACC-3'
	3'-CATTC ATGGAGGGGTCGAGTTA  AGGTGAGTGTGTTGGTGTCTAT  TGGTCCGGTTTTGTCTT CATGG-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 002-204001007
Forward Primer: 5'-AATTTATGGGCTCTGTGCCGTGC-3' Tm: 59.148
Reverse Primer: 5'-CCCAGTGCTAAGTTGAGTGTGGC-3' Tm: 61.088
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 135, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr2:204001112-204001167

Rank: 8	Homology Score: 13
	Genomic Region: Intergenic	Closest Gene: Atpbd4
Match Type: R15L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-CATAA TTCAATGTTGTTTGGGC     TTTTTTTTTTTTTTT      TTGGAGCTGGGGACGAA CCCAG-3'
	3'-GTATT AAGTTACAACAAACCCG     AAAAAAAAAAAAAAA      AACCTCGACCCCTGCTT GGGTC-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 003-100952790
Forward Primer: 5'-GCCATGAAGAAGACATGCAGGAG-3' Tm: 59.148
Reverse Primer: 5'-GGATCACTTGAAATGTGGAGCTTCCTAC-3' Tm: 60.298
Expected PCR Product Length: 405
Expected CelI Cleavage Product Sizes: 218, 187
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr3:100952982-100953030

Rank: 9	Homology Score: 13
	Genomic Region: Intergenic	Closest Gene: Fgf13
Match Type: L18R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-CCTCG TTCCTGCTCTTCTCCAT    GGACCACTTTGATGAGAT    AAAAGAACAACACCCAA ACTGG-3'
	3'-GGAGC AAGGACGAGAAGAGGTA    CCTGGTGAAACTACTCTA    TTTTCTTGTTGTGGGTT TGACC-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 00X-144712285
Forward Primer: 5'-CCAAGCGAGACAGAGATCGG-3' Tm: 59.508
Reverse Primer: 5'-GGTACTTGGCCCTCAGAATCG-3' Tm: 59.378
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 135, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chrX:144712392-144712443

Rank: 10	Homology Score: 13
	Genomic Region: Intergenic	Closest Gene: Ifnar1
Match Type: L15R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-CCTTG TTCCTTCCCAGCTGCCT     AGGAATGTGGGACCA      ACCTGAGCAACACAGCA AGAGC-3'
	3'-GGAAC AAGGAAGGGTCGACGGA     TCCTTACACCCTGGT      TGGACTCGTTGTGTCGT TCTCG-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 011-031478392
Forward Primer: 5'-GATCTGATCCCCTTTTCTGGTGTG-3' Tm: 59.038
Reverse Primer: 5'-CTTCTTTAGCACGTCGCCCC-3' Tm: 59.508
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 134, 136
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr11:31478500-31478548

Rank: 11	Homology Score: 10
	Genomic Region: Exon	Closest Gene: Lrp1
Match Type: R20L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-GCTGT TTCAATCTTGGGGTGGT   TCCCCCAGTCTGACCAGTAC   ATGGTGCTGGGAAGGGA GAGAT-3'
	3'-CGACA AAGTTAGAACCCCACCA   AGGGGGTCAGACTGGTCATG   TACCACGACCCTTCCCT CTCTA-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 007-067524783
Forward Primer: 5'-TGATCAGAGCCTGCCCCTTC-3' Tm: 59.508
Reverse Primer: 5'-GACAGGGTGCTTCCTCACAATAC-3' Tm: 59.148
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 138, 132
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr7:67524892-67524945

Rank: 12	Homology Score: 10
	Genomic Region: Intron	Closest Gene: Pcbp3
Match Type: R21L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-AGGCC TTGAGTTTTGATCTGGT  CTCCCTTGATGGAAATAAAGC   AAGATGCTGGGCCAGAA TCCAT-3'
	3'-TCCGG AACTCAAAACTAGACCA  GAGGGAACTACCTTTATTTCG   TTCTACGACCCGGTCTT AGGTA-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 020-012161079
Forward Primer: 5'-CCGTTGCCCGAAAAGCACCT-3' Tm: 59.508
Reverse Primer: 5'-GAACCCATTTGCTCTCAGCCTTG-3' Tm: 59.148
Expected PCR Product Length: 320
Expected CelI Cleavage Product Sizes: 168, 152
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr20:12161218-12161272

Rank: 13	Homology Score: 10
	Genomic Region: Intron	Closest Gene: Tm6sf1
Match Type: R13L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-CTTTG TTCAGTTTTTTTTTTTT      TTTTTTTCTTTTT       TTGGAGCTGGGAACGAA CCCAG-3'
	3'-GAAAC AAGTCAAAAAAAAAAAA      AAAAAAAGAAAAA       AACCTCGACCCTTGCTT GGGTC-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. Warning! The sequence of this region of the genome is poorly mapped. 
Primer Index: 001-138061468
Forward Primer: 5'-CCGTGAAGCAGGTCACAAAGC-3' Tm: 59.378
Reverse Primer: 5'-CAGCAAGGGAAGTGCTACTTGATG-3' Tm: 59.038
Expected PCR Product Length: 303
Expected CelI Cleavage Product Sizes: 164, 139
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr1:138061607-138061653

Rank: 14	Homology Score: 10
	Genomic Region: Intron	Closest Gene: Tsc2
Match Type: L18R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-TTAGC TACCTGTCCAGCACCAT    GTCTGCCTGGTGCCTCCC    ACCATGACAATAATGGA CTAAA-3'
	3'-AATCG ATGGACAGGTCGTGGTA    CAGACGGACCACGGAGGG    TGGTACTGTTATTACCT GATTT-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 010-013862379
Forward Primer: 5'-CATAGGTGGGGCCTTATCAGAG-3' Tm: 59.258
Reverse Primer: 5'-GGTCCCTAGTCAAGGACATTGTCT-3' Tm: 59.038
Expected PCR Product Length: 273
Expected CelI Cleavage Product Sizes: 131, 142
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr10:13862482-13862533

Rank: 15	Homology Score: 10
	Genomic Region: Intron	Closest Gene: Cntn4
Match Type: L20R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-TTATC TACCTTCCCAGCCCCAT   TATGCATGGGTACAACATAA   ACCAACACAAAACAAAA GCAAA-3'
	3'-AATAG ATGGAAGGGTCGGGGTA   ATACGTACCCATGTTGTATT   TGGTTGTGTTTTGTTTT CGTTT-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 004-141883124
Forward Primer: 5'-AATCCGCAGTCTAAGCCAACAGAG-3' Tm: 59.038
Reverse Primer: 5'-TGCTAGGCAAGCACTCTACCAC-3' Tm: 59.258
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 134, 136
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr4:141883229-141883282

Rank: 16	Homology Score: 10
	Genomic Region: Intron	Closest Gene: Asip
Match Type: L22R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-AGGTT TCCCTGCGCTGCCTCAT  GATTAAGTTAACATTATTTTTA  GCAAGAACAACACAGAA GGGAT-3'
	3'-TCCAA AGGGACGCGACGGAGTA  CTAATTCAATTGTAATAAAAAT  CGTTCTTGTTGTGTCTT CCCTA-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 003-145456877
Forward Primer: 5'-GGCGACAGTTCCTCTGTTTTTGC-3' Tm: 59.148
Reverse Primer: 5'-CACAGTAGTGGCTGTAGGCATTTG-3' Tm: 59.038
Expected PCR Product Length: 375
Expected CelI Cleavage Product Sizes: 173, 202
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr3:145457020-145457075

Rank: 17	Homology Score: 10
	Genomic Region: Intron	Closest Gene: Mad1l1
Match Type: R13L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-GCAGC TTTAGTCTTGTTCTGTT      GTGATGTTTTGTT       TTGGAGATGCCAAGGAA CAAAC-3'
	3'-CGTCG AAATCAGAACAAGACAA      CACTACAAAACAA       AACCTCTACGGTTCCTT GTTTG-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 012-015035708
Forward Primer: 5'-AGCCTCCTGGTTACGTCCCAA-3' Tm: 59.378
Reverse Primer: 5'-TGACTGCTGTGTGTGGGGAG-3' Tm: 59.508
Expected PCR Product Length: 309
Expected CelI Cleavage Product Sizes: 137, 172
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr12:15035820-15035866

Rank: 18	Homology Score: 10
	Genomic Region: Intron	Closest Gene: Snx7
Match Type: R15L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-CAACT TTCTGTGTGGATATGGC     GATGACTAACTGGGC      ATGGAGCATGACAGGAA AAATT-3'
	3'-GTTGA AAGACACACCTATACCG     CTACTGATTGACCCG      TACCTCGTACTGTCCTT TTTAA-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 002-214232497
Forward Primer: 5'-CACGTGTGTGCCTCAGTAACAG-3' Tm: 59.258
Reverse Primer: 5'-AGCAGGCCCTGGAACAGTTCT-3' Tm: 59.378
Expected PCR Product Length: 310
Expected CelI Cleavage Product Sizes: 179, 131
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr2:214232650-214232698

Rank: 19	Homology Score: 10
	Genomic Region: Intron	Closest Gene: Fra10ac1
Match Type: L22R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-TCAAG TTCCTAGCCAGCTTCAT  TCACAGAGCAAGTTCCAGGACA  GCCAGGGCTACACAGAA AAACT-3'
	3'-AGTTC AAGGATCGGTCGAAGTA  AGTGTCTCGTTCAAGGTCCTGT  CGGTCCCGATGTGTCTT TTTGA-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 001-242536006
Forward Primer: 5'-TGGAGGTTGGGAGAGTAGAGG-3' Tm: 59.378
Reverse Primer: 5'-TGAAGGAGGGAACACTCAGCAG-3' Tm: 59.258
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 135, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr1:242536111-242536166

Rank: 20	Homology Score: 10
	Genomic Region: Intron	Closest Gene: Ube2j1
Match Type: L21R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-TTGCT TTCCTTTCCAGCTCCTT  CTGCCTCTCTTAGGCATTTCC   AGCAGAGCCACTCTGCA ATGGT-3'
	3'-AACGA AAGGAAAGGTCGAGGAA  GACGGAGAGAATCCGTAAAGG   TCGTCTCGGTGAGACGT TACCA-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 005-049320556
Forward Primer: 5'-CTGTGGTGAAGGTGTTACTGTGTC-3' Tm: 59.038
Reverse Primer: 5'-GAGAAGGCTGGACCGAATAGAAG-3' Tm: 59.148
Expected PCR Product Length: 292
Expected CelI Cleavage Product Sizes: 161, 131
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr5:49320688-49320742

Rank: 21	Homology Score: 10
	Genomic Region: Intron	Closest Gene: Ralgapa1
Match Type: L13R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-TCTCC TTCCTTTCCAGCTCCCT      TCTTTCTTTCATT       ACTAGATATGCACTGAA AGCTG-3'
	3'-AGAGG AAGGAAAGGTCGAGGGA      AGAAAGAAAGTAA       TGATCTATACGTGACTT TCGAC-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 006-075907048
Forward Primer: 5'-GGTGTCCTCATTCACCTCTGACAA-3' Tm: 59.038
Reverse Primer: 5'-GTGTTTGGAACATCCTCTGCGAC-3' Tm: 59.148
Expected PCR Product Length: 363
Expected CelI Cleavage Product Sizes: 189, 174
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr6:75907212-75907258

Rank: 22	Homology Score: 10
	Genomic Region: Intron	Closest Gene: Rnf4
Match Type: L19R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-GTAGA TCCCTCCCCAGCTCCCT   TGTCAGGACCTAGCCAGGT    ACATGATCAACATTCAA GAAAA-3'
	3'-CATCT AGGGAGGGGTCGAGGGA   ACAGTCCTGGATCGGTCCA    TGTACTAGTTGTAAGTT CTTTT-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 014-082098705
Forward Primer: 5'-AGCCAAGGAAGAAACCTGTGCAGC-3' Tm: 60.908
Reverse Primer: 5'-CTGTGGATGGCTAGAGTGTACTCA-3' Tm: 59.038
Expected PCR Product Length: 300
Expected CelI Cleavage Product Sizes: 130, 170
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr14:82098807-82098859

Rank: 23	Homology Score: 10
	Genomic Region: Intron	Closest Gene: Emid1
Match Type: L15R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-AACAT TCCCTGTCCAGCTCCAC     CTCTTGGAAACCTTG      AGGAAAACAACCCTGCA GACTT-3'
	3'-TTGTA AGGGACAGGTCGAGGTG     GAGAACCTTTGGAAC      TCCTTTTGTTGGGACGT CTGAA-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 014-085768096
Forward Primer: 5'-GGGAATGGTTAGCCCCATCTC-3' Tm: 59.378
Reverse Primer: 5'-CATGAGGAAACAGTTCAGGAGGG-3' Tm: 59.148
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 136, 134
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr14:85768206-85768254

Rank: 24	Homology Score: 10
	Genomic Region: Intergenic	Closest Gene: Tacstd2
Match Type: L24R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-TTTCC TTCATTCTCTGCTTCAT TCATTGTGTCTGCATTTCCTTTAG ACAGGAACAACTCTGAA TTATT-3'
	3'-AAAGG AAGTAAGAGACGAAGTA AGTAACACAGACGTAAAGGAAATC TGTCCTTGTTGAGACTT AATAA-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 004-100499585
Forward Primer: 5'-GGCATAGCCTCTCAGGAAACAG-3' Tm: 59.258
Reverse Primer: 5'-CTTCCAGGACAGAACAGGAAAGAC-3' Tm: 59.038
Expected PCR Product Length: 336
Expected CelI Cleavage Product Sizes: 165, 171
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr4:100499719-100499776

Rank: 25	Homology Score: 10
	Genomic Region: Intergenic	Closest Gene: Tbl1xr1
Match Type: R22L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-ACATC TCCAGTCTTATTCTGGT  CTATTATCAACAGTAAGGGAAA  ATGAAGCTGCCATGGAA AGTAC-3'
	3'-TGTAG AGGTCAGAATAAGACCA  GATAATAGTTGTCATTCCCTTT  TACTTCGACGGTACCTT TCATG-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 002-106965669
Forward Primer: 5'-ATAGTGGCCAGTGCCTGGAC-3' Tm: 59.508
Reverse Primer: 5'-CCAGGAGAAAAGGCCATCTTCCA-3' Tm: 59.148
Expected PCR Product Length: 307
Expected CelI Cleavage Product Sizes: 131, 176
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr2:106965770-106965825

Rank: 26	Homology Score: 10
	Genomic Region: Intergenic	Closest Gene: MGC125002
Match Type: R23L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-TTTGA TCCAGAATTGTTCTGGT CTAAAAGAAATGTGGTGATAAAA  ATGGAGCAGAGATTGAA GGAAA-3'
	3'-AAACT AGGTCTTAACAAGACCA GATTTTCTTTACACCACTATTTT  TACCTCGTCTCTAACTT CCTTT-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 005-113632907
Forward Primer: 5'-GGAGGAATCTCTAGAAAGTCCCAG-3' Tm: 59.038
Reverse Primer: 5'-GGCTCCTATCTGCAGGTCCA-3' Tm: 59.508
Expected PCR Product Length: 339
Expected CelI Cleavage Product Sizes: 208, 131
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr5:113633085-113633141

Rank: 27	Homology Score: 10
	Genomic Region: Intergenic	Closest Gene: LOC688539
Match Type: R18L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-CCAGT TTGGGTGTTTTTCTTTT    ATCTGGTCAAAGTGATCC    ATGGAGAAGAGCAGGAA TGAGG-3'
	3'-GGTCA AACCCACAAAAAGAAAA    TAGACCAGTTTCACTAGG    TACCTCTTCTCGTCCTT ACTCC-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 00X-117110415
Forward Primer: 5'-CTTTGCCCACAGAATAGCAGCC-3' Tm: 59.258
Reverse Primer: 5'-CTAGCCAAGCGCGACAAAGTTC-3' Tm: 59.258
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 135, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chrX:117110522-117110573

Rank: 28	Homology Score: 10
	Genomic Region: Intergenic	Closest Gene: Pde4b
Match Type: R15L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-TTTCG TCCATTATTGTCATGGT     GTCATGCAAGGTGAC      ATGGTGCTGGGGAGGCA GCTGA-3'
	3'-AAAGC AGGTAATAACAGTACCA     CAGTACGTTCCACTG      TACCACGACCCCTCCGT CGACT-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 005-123636672
Forward Primer: 5'-CTCTGAGTGACACGAATTAGTGGG-3' Tm: 59.038
Reverse Primer: 5'-GGAGTGTGTCACTAAGGCTGG-3' Tm: 59.378
Expected PCR Product Length: 350
Expected CelI Cleavage Product Sizes: 218, 132
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr5:123636864-123636912

Rank: 29	Homology Score: 10
	Genomic Region: Intergenic	Closest Gene: Mctp2
Match Type: R14L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-AATCA TTCAGTGAATTTGTTGT      GTCACATCTGCCCC      ATGGAGGTGGGAGGGAA GAATA-3'
	3'-TTAGT AAGTCACTTAAACAACA      CAGTGTAGACGGGG      TACCTCCACCCTCCCTT CTTAT-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 001-127719260
Forward Primer: 5'-CCAACATTCTCCCAAGAGATTCTGGAAAC-3' Tm: 60.178
Reverse Primer: 5'-GTTCCAGGTCAACCAGGACTAC-3' Tm: 59.258
Expected PCR Product Length: 368
Expected CelI Cleavage Product Sizes: 135, 233
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr1:127719369-127719416

Rank: 30	Homology Score: 10
	Genomic Region: Intergenic	Closest Gene: Ocrl
Match Type: R18L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-CCAGT TTGGGTGTTGTTCTTTC    AGCTGGTCAAAGTGGTCC    ATGGAGAAGAGCAGGAA TGAGG-3'
	3'-GGTCA AACCCACAACAAGAAAG    TCGACCAGTTTCACCAGG    TACCTCTTCTCGTCCTT ACTCC-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 00X-133545933
Forward Primer: 5'-CGCTCTTCAAGGTATTTTGCCCAC-3' Tm: 59.038
Reverse Primer: 5'-CTAGCCAAACACGACAAAGATCGG-3' Tm: 59.038
Expected PCR Product Length: 281
Expected CelI Cleavage Product Sizes: 149, 132
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chrX:133546054-133546105

Rank: 31	Homology Score: 10
	Genomic Region: Intergenic	Closest Gene: Zfand6
Match Type: R15L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-AGGAG TTCAGTCATGTTCAAGG     GCACTTCTTGTGTAA      ATGGGGCTGGACAGGGA GGAGT-3'
	3'-TCCTC AAGTCAGTACAAGTTCC     CGTGAAGAACACATT      TACCCCGACCTGTCCCT CCTCA-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 001-140972716
Forward Primer: 5'-GGGACCACCTCAGCTAAGGA-3' Tm: 59.508
Reverse Primer: 5'-GTGGGGTTGTTGGTTTTCAGCTC-3' Tm: 59.148
Expected PCR Product Length: 368
Expected CelI Cleavage Product Sizes: 173, 195
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr1:140972863-140972911

Rank: 32	Homology Score: 10
	Genomic Region: Intergenic	Closest Gene: Mir3589
Match Type: R22L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-CTTTG TAGACTGTGGTTCTGCT  GATTGTGGGATGACAGAAAGGA  ATGGAGATGGGCTAGAA GTCCA-3'
	3'-GAAAC ATCTGACACCAAGACGA  CTAACACCCTACTGTCTTTCCT  TACCTCTACCCGATCTT CAGGT-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 00X-146103263
Forward Primer: 5'-CTACACTCCTTTTTGTTGGGTGGG-3' Tm: 59.038
Reverse Primer: 5'-CTCTGCTGCAGAGCCTTTTTTCC-3' Tm: 59.148
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 136, 134
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chrX:146103369-146103424

Rank: 33	Homology Score: 10
	Genomic Region: Intergenic	Closest Gene: Mafb
Match Type: R16L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-CTTTC TGCTGTGTTTTTCTGGT     TTCATTTGGATGTGAG     ATGGAGCTGAACTGTGA TTTCA-3'
	3'-GAAAG ACGACACAAAAAGACCA     AAGTAAACCTACACTC     TACCTCGACTTGACACT AAAGT-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 003-150734535
Forward Primer: 5'-CACCAGCCAAGAACGGCGTCTTT-3' Tm: 61.088
Reverse Primer: 5'-CACCTCTGCACCTCCTTCTAAC-3' Tm: 59.258
Expected PCR Product Length: 323
Expected CelI Cleavage Product Sizes: 131, 192
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr3:150734639-150734688

Rank: 34	Homology Score: 10
	Genomic Region: Intergenic	Closest Gene: Uncx
Match Type: L23R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-GAGTC TTTCTGCCCAGCTCTAG CTGTCCTGGAACTCACCCTGTAG  ACCAGGCCAGCCTTGAA CTCAC-3'
	3'-CTCAG AAAGACGGGTCGAGATC GACAGGACCTTGAGTGGGACATC  TGGTCCGGTCGGAACTT GAGTG-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 012-015478690
Forward Primer: 5'-CCACTCAAGACAAGCAGAAAGGAC-3' Tm: 59.038
Reverse Primer: 5'-CAAAACAGCAGCAGCAAAAGCTGG-3' Tm: 59.038
Expected PCR Product Length: 278
Expected CelI Cleavage Product Sizes: 147, 131
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr12:15478807-15478863

Rank: 35	Homology Score: 10
	Genomic Region: Intergenic	Closest Gene: Fundc1
Match Type: L19R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-AGACC TTCCTAACCCTTTCCAT   TTGCTTAAATTAACAGAGA    AACAGAACAATACTCAA AATTG-3'
	3'-TCTGG AAGGATTGGGAAAGGTA   AACGAATTTAATTGTCTCT    TTGTCTTGTTATGAGTT TTAAC-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 00X-016509931
Forward Primer: 5'-CTACCAATGTCTATGGAGTGACCG-3' Tm: 59.038
Reverse Primer: 5'-GCCTAGAGTATCCCAAATGTGCAC-3' Tm: 59.038
Expected PCR Product Length: 365
Expected CelI Cleavage Product Sizes: 130, 235
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chrX:16510033-16510085

Rank: 36	Homology Score: 10
	Genomic Region: Intergenic	Closest Gene: LOC679825
Match Type: R16L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-TGAGG TTTACTTTTGCTGTGGT     AAAACATCATGACCAA     AGGGACTTGGGCAGGAA AGAGT-3'
	3'-ACTCC AAATGAAAACGACACCA     TTTTGTAGTACTGGTT     TCCCTGAACCCGTCCTT TCTCA-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 001-001765937
Forward Primer: 5'-TTCTAGCCAAGAGGTCCAAAGGCC-3' Tm: 60.908
Reverse Primer: 5'-TGCATGGCCTCTGCATCAGC-3' Tm: 59.508
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 135, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr1:1766045-1766094

Rank: 37	Homology Score: 10
	Genomic Region: Intergenic	Closest Gene: Mir206
Match Type: L23R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-TTTTA TTAGTGCCCAAATCTAT AGTAGTAATGGTCTAATTTGAGC  TCCAGAATAACTCTGAA TCAAA-3'
	3'-AAAAT AATCACGGGTTTAGATA TCATCATTACCAGATTAAACTCG  AGGTCTTATTGAGACTT AGTTT-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 009-019047855
Forward Primer: 5'-GTGGCTTTGGTCCTCATCTGTC-3' Tm: 59.258
Reverse Primer: 5'-CCTCTTTCGGAGATAGGCATGTTC-3' Tm: 59.038
Expected PCR Product Length: 302
Expected CelI Cleavage Product Sizes: 161, 141
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr9:19047986-19048042

Rank: 38	Homology Score: 10
	Genomic Region: Intergenic	Closest Gene: Rn45s
Match Type: L23R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-GGATC TGCCTTCTCAGATCCAA ATCCTCCATTTCAAGATGCAAGA  AGCAGAGCCACACTGAA AGCTG-3'
	3'-CCTAG ACGGAAGAGTCTAGGTT TAGGAGGTAAAGTTCTACGTTCT  TCGTCTCGGTGTGACTT TCGAC-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 0Un-021787610
Forward Primer: 5'-CCTGGGATGTGGTGTAAGTTGG-3' Tm: 59.258
Reverse Primer: 5'-GAGGAGCCAAATCTGGACCATTAG-3' Tm: 59.038
Expected PCR Product Length: 273
Expected CelI Cleavage Product Sizes: 138, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chrUn:21787718-21787774

Rank: 39	Homology Score: 10
	Genomic Region: Intergenic	Closest Gene: Cntnap5b
Match Type: L22R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-CTCTT TCTCTTCCTAGCTCCCT  GCAATTGTATCCTGCTGTGAGT  ACCAGATCAAAAGTGAA TATTT-3'
	3'-GAGAA AGAGAAGGATCGAGGGA  CGTTAACATAGGACGACACTCA  TGGTCTAGTTTTCACTT ATAAA-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 013-002441765
Forward Primer: 5'-CTGTCTCTGTCTCTCTTAGTTCCC-3' Tm: 59.038
Reverse Primer: 5'-CCCTTTTTGCCTTTCCATGCAGTG-3' Tm: 59.038
Expected PCR Product Length: 336
Expected CelI Cleavage Product Sizes: 177, 159
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr13:2441912-2441967

Rank: 40	Homology Score: 10
	Genomic Region: Intergenic	Closest Gene: Mybpc1
Match Type: R22L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-GGTCT TTCTGTGTAGTCCTGGT  TGTCTTGGAATTCTCTATGTAG  ATCAAGCTGGCCCGAAA CTCAT-3'
	3'-CCAGA AAGACACATCAGGACCA  ACAGAACCTTAAGAGATACATC  TAGTTCGACCGGGCTTT GAGTA-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 007-025308852
Forward Primer: 5'-CCAGTCTGATGGTTATACATACAGTGCAGT-3' Tm: 60.058
Reverse Primer: 5'-GGTTTTCAACCTGAGTGGGAGAAG-3' Tm: 59.038
Expected PCR Product Length: 358
Expected CelI Cleavage Product Sizes: 220, 138
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr7:25309042-25309097

Rank: 41	Homology Score: 10
	Genomic Region: Intergenic	Closest Gene: Mir532
Match Type: L21R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-TTTTT TTCCTACCCACCCCCAT  GCCTCCCCAGCCTTGGATGAA   ACAGGAACAAAAGTCAA AATTT-3'
	3'-AAAAA AAGGATGGGTGGGGGTA  CGGAGGGGTCGGAACCTACTT   TGTCCTTGTTTTCAGTT TTAAA-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 00X-027254372
Forward Primer: 5'-CCATTATCGCTAGCCTAGCTTACG-3' Tm: 59.038
Reverse Primer: 5'-GACTGGGAATAAGTGGTGTGGTAC-3' Tm: 59.038
Expected PCR Product Length: 301
Expected CelI Cleavage Product Sizes: 167, 134
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chrX:27254510-27254564

Rank: 42	Homology Score: 10
	Genomic Region: Intergenic	Closest Gene: Cldn8
Match Type: R21L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-CTCTG TTCTGTGTTCTGTTGGG  AGTTAAATCAAACGTATTTTC   ATGGAGCTTGGCATGTA GAAAA-3'
	3'-GAGAC AAGACACAAGACAACCC  TCAATTTAGTTTGCATAAAAG   TACCTCGAACCGTACAT CTTTT-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 011-028431745
Forward Primer: 5'-GCTCAGAACAGCTAATGTGAGTGC-3' Tm: 59.038
Reverse Primer: 5'-CAATGACTGTCCAGAACTGAGGC-3' Tm: 59.148
Expected PCR Product Length: 314
Expected CelI Cleavage Product Sizes: 177, 137
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr11:28431893-28431947

Rank: 43	Homology Score: 10
	Genomic Region: Intergenic	Closest Gene: Tmem132b
Match Type: R24L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-GTTGT TTTGTTGTTGTTTTGTT TTTTTTGTTTTGTTTTTTAGATTC ATTTAGCTGGACAGGAA GAGTA-3'
	3'-CAACA AAACAACAACAAAACAA AAAAAACAAAACAAAAAATCTAAG TAAATCGACCTGTCCTT CTCAT-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 012-031781347
Forward Primer: 5'-CGCGTAATACGCCTTTTGTTGACC-3' Tm: 59.038
Reverse Primer: 5'-GAGCAGTGAACAGCATTAGCTTCC-3' Tm: 59.038
Expected PCR Product Length: 368
Expected CelI Cleavage Product Sizes: 232, 136
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr12:31781548-31781605

Rank: 44	Homology Score: 10
	Genomic Region: Intergenic	Closest Gene: Cav2
Match Type: L23R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-GGATC TGCCTTCTCAGATCCAA ATCCTCCTTTTCAAGATGCAAGA  AGCAGAGCCACACTGAA AGCTG-3'
	3'-CCTAG ACGGAAGAGTCTAGGTT TAGGAGGAAAAGTTCTACGTTCT  TCGTCTCGGTGTGACTT TCGAC-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: dom-003376644
Forward Primer: 5'-CCTGGGATGTGGTGTAAGTTGG-3' Tm: 59.258
Reverse Primer: 5'-GAGGAGCCAAATCTGGACCATTAG-3' Tm: 59.038
Expected PCR Product Length: 273
Expected CelI Cleavage Product Sizes: 138, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chrUn_random:3376752-3376808

Rank: 45	Homology Score: 10
	Genomic Region: Intergenic	Closest Gene: Dpp6
Match Type: R15L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-GTCAG TTCTGTGCTGTGCTCAT     GCCATTGTGGGGAAG      AAGGAGATGGGAAGGAA GGTGC-3'
	3'-CAGTC AAGACACGACACGAGTA     CGGTAACACCCCTTC      TTCCTCTACCCTTCCTT CCACG-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 004-003843423
Forward Primer: 5'-GGTGACCAATACCAACCATCAGG-3' Tm: 59.148
Reverse Primer: 5'-CCTCCTTTCAGGAAGGCTCTTTCT-3' Tm: 59.038
Expected PCR Product Length: 319
Expected CelI Cleavage Product Sizes: 169, 150
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr4:3843566-3843614

Rank: 46	Homology Score: 10
	Genomic Region: Intergenic	Closest Gene: LOC367515
Match Type: L23R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-GGATC TGCCTTCTCAGATCCAA ATCCTCCATTTCAAGATGCAAGA  AGCAGAGCCACACTGAA AGCTG-3'
	3'-CCTAG ACGGAAGAGTCTAGGTT TAGGAGGTAAAGTTCTACGTTCT  TCGTCTCGGTGTGACTT TCGAC-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. Warning! The sequence of this region of the genome is poorly mapped. 
Primer Index: 0Un-038608458
Forward Primer: 5'-CCTGGGATGTGGTGTAAGTTGG-3' Tm: 59.258
Reverse Primer: 5'-GAGACAACATACCCTGCTCTCAC-3' Tm: 59.148
Expected PCR Product Length: 316
Expected CelI Cleavage Product Sizes: 138, 178
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chrUn:38608566-38608622

Rank: 47	Homology Score: 10
	Genomic Region: Intergenic	Closest Gene: Gabra2
Match Type: R19L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-GCTGT TGCAGTTTAGTATTGGT   ACGGAGGTCCTGGGAAGGC    ATTGCTCTGGGCAGGAA ATAAG-3'
	3'-CGACA ACGTCAAATCATAACCA   TGCCTCCAGGACCCTTCCG    TAACGAGACCCGTCCTT TATTC-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 014-039438747
Forward Primer: 5'-GCTCCATAAACTGGGCCTGAGA-3' Tm: 59.258
Reverse Primer: 5'-GCTAGCCTCTACCTCTTCCTG-3' Tm: 59.378
Expected PCR Product Length: 342
Expected CelI Cleavage Product Sizes: 144, 198
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr14:39438863-39438915

Rank: 48	Homology Score: 10
	Genomic Region: Intergenic	Closest Gene: E2f3
Match Type: R20L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-TTTTG TGCTGTGCTGTGCTGGG   CACAGAACCCAGGGCAGAGG   AGCGTGCTGGGCAGGAA CCCAA-3'
	3'-AAAAC ACGACACGACACGACCC   GTGTCTTGGGTCCCGTCTCC   TCGCACGACCCGTCCTT GGGTT-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 017-041218534
Forward Primer: 5'-CCAGGCCATGATTATTCTGCAGG-3' Tm: 59.148
Reverse Primer: 5'-TCCCAAGTCACCTGCAAGCC-3' Tm: 59.508
Expected PCR Product Length: 343
Expected CelI Cleavage Product Sizes: 212, 131
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr17:41218717-41218770

Rank: 49	Homology Score: 10
	Genomic Region: Intergenic	Closest Gene: Grxcr1
Match Type: L22R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-TACTT TTCCCGTCCACCTCCAT  GTGTTGATTCTTGCTGTTCAAA  AACAAAACAAAACAAAA CAAAA-3'
	3'-ATGAA AAGGGCAGGTGGAGGTA  CACAACTAAGAACGACAAGTTT  TTGTTTTGTTTTGTTTT GTTTT-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 014-042588777
Forward Primer: 5'-GAGACTACAGATGCCAAAAGTTTCCATACC-3' Tm: 60.058
Reverse Primer: 5'-ACACACACACACACACACACACAC-3' Tm: 59.038
Expected PCR Product Length: 411
Expected CelI Cleavage Product Sizes: 281, 130
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr14:42589028-42589083

Rank: 50	Homology Score: 10
	Genomic Region: Intergenic	Closest Gene: Plcl2
Match Type: R21L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-ATGTT TTCAGTGTTGCTCCTGT  TGGGTAAAGGAGGAATGTTCC   CTGGATCGGGGCTGGTA TAGTG-3'
	3'-TACAA AAGTCACAACGAGGACA  ACCCATTTCCTCCTTACAAGG   GACCTAGCCCCGACCAT ATCAC-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 009-004673112
Forward Primer: 5'-AATCCACCGAGCCTAGTAACTGC-3' Tm: 59.148
Reverse Primer: 5'-TACACTGCGCTCACACAGCAAG-3' Tm: 59.258
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 135, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr9:4673218-4673272

Rank: 51	Homology Score: 10
	Genomic Region: Intergenic	Closest Gene: Adrbk2
Match Type: L23R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-GGATC TGCCTTCTCAGATCCAA ATCCTCCATTTCAAGATGCAAGA  AGCAGAGCCACACTGAA AGCTG-3'
	3'-CCTAG ACGGAAGAGTCTAGGTT TAGGAGGTAAAGTTCTACGTTCT  TCGTCTCGGTGTGACTT TCGAC-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 0Un-051334472
Forward Primer: 5'-CCTGGGATGTGGTGTAAGTTGG-3' Tm: 59.258
Reverse Primer: 5'-GAGGAGCCAAATCTGGACCATTAG-3' Tm: 59.038
Expected PCR Product Length: 273
Expected CelI Cleavage Product Sizes: 138, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chrUn:51334580-51334636

Rank: 52	Homology Score: 10
	Genomic Region: Intergenic	Closest Gene: Cbx8
Match Type: R20L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-AAACA TTCAGTGTTGGACTGGC   AAGATGGCTCACTGGGTGAA   AGGGAGCTTGCCACCAA GCTTG-3'
	3'-TTTGT AAGTCACAACCTGACCG   TTCTACCGAGTGACCCACTT   TCCCTCGAACGGTGGTT CGAAC-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 0Un-051940753
Forward Primer: 5'-CAAACAGAGCCAGGTGTGGTG-3' Tm: 59.378
Reverse Primer: 5'-GCCCTCTGTGTCATGCCTTATTAC-3' Tm: 59.038
Expected PCR Product Length: 355
Expected CelI Cleavage Product Sizes: 163, 192
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chrUn:51940887-51940940

Rank: 53	Homology Score: 10
	Genomic Region: Intergenic	Closest Gene: Pard3
Match Type: R12L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-TTTGC TTTTTTATTGTTCTGGA       AGTTAAGTTTGA       ATGGGGCTTGGTAGGAA ATGAT-3'
	3'-AAACG AAAAAATAACAAGACCT       TCAATTCAAACT       TACCCCGAACCATCCTT TACTA-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 019-057896612
Forward Primer: 5'-CCCAGTGCTGGGGTTACAAACA-3' Tm: 59.258
Reverse Primer: 5'-TCTTAGCCAGGAATGAGGCTGC-3' Tm: 59.258
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 138, 132
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr19:57896725-57896770

Rank: 54	Homology Score: 10
	Genomic Region: Intergenic	Closest Gene: Zfp385b
Match Type: L19R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-TTTCC TTCCAGCCCATCTCCAC   TTCTTTATGCCAGCACTGA    TCCACAGAAACACTGTA AACCA-3'
	3'-AAAGG AAGGTCGGGTAGAGGTG   AAGAAATACGGTCGTGACT    AGGTGTCTTTGTGACAT TTGGT-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 003-060396362
Forward Primer: 5'-CCACTGGGAGTGTATTCGGTATG-3' Tm: 59.148
Reverse Primer: 5'-GTAAACAGGCCTCTACAGAACGG-3' Tm: 59.148
Expected PCR Product Length: 362
Expected CelI Cleavage Product Sizes: 230, 132
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr3:60396564-60396616

Rank: 55	Homology Score: 10
	Genomic Region: Intergenic	Closest Gene: LOC302022
Match Type: L23R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-GGATC TGCCTTCTCAGATCCAA ATCCTCCATTTCAAGATGCAAGA  AGCAGAGCCACACTGAA AGCTG-3'
	3'-CCTAG ACGGAAGAGTCTAGGTT TAGGAGGTAAAGTTCTACGTTCT  TCGTCTCGGTGTGACTT TCGAC-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 0Un-061148009
Forward Primer: 5'-CCTGGGATGTGGTGTAAGTTGG-3' Tm: 59.258
Reverse Primer: 5'-GAGACAACATACCCTGCTCTCAC-3' Tm: 59.148
Expected PCR Product Length: 316
Expected CelI Cleavage Product Sizes: 138, 178
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chrUn:61148117-61148173

Rank: 56	Homology Score: 10
	Genomic Region: Intergenic	Closest Gene: Map2
Match Type: R13L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-ATTTA TTCATTGTTGTTCTTGG      TGGCTGCGTTATC       AGTGTTCTGGTCAGGAA GTTGT-3'
	3'-TAAAT AAGTAACAACAAGAACC      ACCGACGCAATAG       TCACAAGACCAGTCCTT CAACA-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 009-065106893
Forward Primer: 5'-CCTCTGTTGGATGTGGAGTTGG-3' Tm: 59.258
Reverse Primer: 5'-CGCACACCTATGGACATCTGAG-3' Tm: 59.258
Expected PCR Product Length: 396
Expected CelI Cleavage Product Sizes: 137, 259
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr9:65107005-65107051

Rank: 57	Homology Score: 10
	Genomic Region: Intergenic	Closest Gene: Irx1
Match Type: R22L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-GGTTT TTCTGTGTAGTTCTGGC  TGTCCTGGAACTGAATCAGTAG  ATGAGGCTGGCCTTGAA CTCAA-3'
	3'-CCAAA AAGACACATCAAGACCG  ACAGGACCTTGACTTAGTCATC  TACTCCGACCGGAACTT GAGTT-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 0Un-075352159
Forward Primer: 5'-CTTGAGAGACTGTGGTGAATGAGG-3' Tm: 59.038
Reverse Primer: 5'-GGAGGAAAAAGGGGACTGTGTTCA-3' Tm: 59.038
Expected PCR Product Length: 298
Expected CelI Cleavage Product Sizes: 150, 148
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chrUn:75352279-75352334

Rank: 58	Homology Score: 10
	Genomic Region: Intergenic	Closest Gene: Grid2
Match Type: R22L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-GGTTT TTCTGTGTAGTTCTGGC  TGTCCTGGAACTGAATCAGTAG  ATGAGGCTGGCCTTGAA CTCAA-3'
	3'-CCAAA AAGACACATCAAGACCG  ACAGGACCTTGACTTAGTCATC  TACTCCGACCGGAACTT GAGTT-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 004-092024652
Forward Primer: 5'-CTTGAGAGACTGTGGTGAATGAGG-3' Tm: 59.038
Reverse Primer: 5'-GGAGGAAAAAGGGGACTGTGTTCA-3' Tm: 59.038
Expected PCR Product Length: 298
Expected CelI Cleavage Product Sizes: 150, 148
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr4:92024772-92024827

Rank: 59	Homology Score: 10
	Genomic Region: Intergenic	Closest Gene: Mir384
Match Type: L18R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-CCTCA TTCCTGCTCTTCTCCAT    GGATCACTTTGACCAGAT    AAAAGAAGAACACCCAA ATTGG-3'
	3'-GGAGT AAGGACGAGAAGAGGTA    CCTAGTGAAACTGGTCTA    TTTTCTTCTTGTGGGTT TAACC-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 00X-093585607
Forward Primer: 5'-CCAAGCGCGACAAAGATCGG-3' Tm: 59.508
Reverse Primer: 5'-GGTACTTTGCCCACAGAATAGCAG-3' Tm: 59.038
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 135, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chrX:93585714-93585765

Rank: 60	Homology Score: 8
	Genomic Region: Exon	Closest Gene: Pik3r5
Match Type: R15L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-TGACT TTCAGTGGTGCTCTGCC     CTAGTGCACCTAGGT      ATGAGGCTGGACAGCAA AGTGC-3'
	3'-ACTGA AAGTCACCACGAGACGG     GATCACGTGGATCCA      TACTCCGACCTGTCGTT TCACG-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 010-055247764
Forward Primer: 5'-TGCTACAAGCCAGAGAAGAGCAG-3' Tm: 59.148
Reverse Primer: 5'-AGACGTCCCTGGTCCATAGC-3' Tm: 59.508
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 131, 139
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr10:55247869-55247917

Rank: 61	Homology Score: 8
	Genomic Region: Intron	Closest Gene: Bai1
Match Type: R20L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-CCATG TTCTGTGTTGTCTTTGT   TTCTTTGTTGTTAAACTGGG   ATGGGGAGGGGCAGGTA CGCTT-3'
	3'-GGTAC AAGACACAACAGAAACA   AAGAAACAACAATTTGACCC   TACCCCTCCCCGTCCAT GCGAA-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 007-112677493
Forward Primer: 5'-TAGGGCAGAGCTGATGGACC-3' Tm: 59.508
Reverse Primer: 5'-CGAGTCTCTTCTTGACTGTTTCCC-3' Tm: 59.038
Expected PCR Product Length: 274
Expected CelI Cleavage Product Sizes: 137, 137
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr7:112677601-112677654

Rank: 62	Homology Score: 8
	Genomic Region: Intron	Closest Gene: Pik3c2a
Match Type: L23R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-GGCTA TTCTAGCCCAGGTCCAG ATGGGTTTATAGTGCAGAATTCT  ACCAGACCTTCAATGAA GACCT-3'
	3'-CCGAT AAGATCGGGTCCAGGTC TACCCAAATATCACGTCTTAAGA  TGGTCTGGAAGTTACTT CTGGA-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 001-174487424
Forward Primer: 5'-GGCCTTAAATGGAATGCATGCTGG-3' Tm: 59.038
Reverse Primer: 5'-CATCCCTGGGATGAAACCTACTTG-3' Tm: 59.038
Expected PCR Product Length: 412
Expected CelI Cleavage Product Sizes: 133, 279
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr1:174487527-174487583

Rank: 63	Homology Score: 8
	Genomic Region: Intron	Closest Gene: Grm8
Match Type: L13R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-ACCCT TGCCTTCCCTGCTCCTT      CTTCTCTGACTGT       TCTAGCACAGCACTGAA AAAGA-3'
	3'-TGGGA ACGGAAGGGACGAGGAA      GAAGAGACTGACA       AGATCGTGTCGTGACTT TTTCT-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 004-054569528
Forward Primer: 5'-CTGTGAGCAAGCCAACAGATAGC-3' Tm: 59.148
Reverse Primer: 5'-CCTTGGAGTTGCTACAGGCTTTG-3' Tm: 59.148
Expected PCR Product Length: 273
Expected CelI Cleavage Product Sizes: 135, 138
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr4:54569638-54569684

Rank: 64	Homology Score: 8
	Genomic Region: Intron	Closest Gene: Map2k1
Match Type: L15R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-CAGCC TTCCTTCCCATCTACAG     AAGAAATCAGCGAAG      AGCAGAACTCCCCTGAA GTCAG-3'
	3'-GTCGG AAGGAAGGGTAGATGTC     TTCTTTAGTCGCTTC      TCGTCTTGAGGGGACTT CAGTC-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 008-068387105
Forward Primer: 5'-CCGACTAGCTTCTCCCATGAC-3' Tm: 59.378
Reverse Primer: 5'-TTACTGTCGGTGTCACAGTGCC-3' Tm: 59.258
Expected PCR Product Length: 322
Expected CelI Cleavage Product Sizes: 191, 131
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr8:68387270-68387318

Rank: 65	Homology Score: 8
	Genomic Region: Intron	Closest Gene: Cdh5
Match Type: R20L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-CTTTA TTCAGGGTTGTTATGTA   GTTACTTTATACTTAGGTAA   CTCTAGGTGGGCAGGAA GGTTT-3'
	3'-GAAAT AAGTCCCAACAATACAT   CAATGAAATATGAATCCATT   GAGATCCACCCGTCCTT CCAAA-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 019-000783634
Forward Primer: 5'-CTCCCCCAAGACACTTTGTCTTAC-3' Tm: 59.038
Reverse Primer: 5'-AAGTATGTGAGAATGCTGCCCAGG-3' Tm: 59.038
Expected PCR Product Length: 273
Expected CelI Cleavage Product Sizes: 143, 130
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr19:783748-783801

Rank: 66	Homology Score: 8
	Genomic Region: Intergenic	Closest Gene: Lamp5
Match Type: L24R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-ATACC TTCCACCCCACCTCCTT CCCCTAGATTTCAAGAAGGGGGGA AGCAGAACAAAACCTAA CCTCG-3'
	3'-TATGG AAGGTGGGGTGGAGGAA GGGGATCTAAAGTTCTTCCCCCCT TCGTCTTGTTTTGGATT GGAGC-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 003-124119953
Forward Primer: 5'-CAGTAGCTGCCTTTGACCAGGA-3' Tm: 59.258
Reverse Primer: 5'-CAAACCAGACAATGGCTTCTGCTC-3' Tm: 59.038
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 140, 130
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr3:124120062-124120119

Rank: 67	Homology Score: 8
	Genomic Region: Intergenic	Closest Gene: Actrt1
Match Type: R14L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-ATTTA TTAATTGTTGGTCTGAT      TACCTATGTTATTA      ATGGTTCTGTTCAGGAA GTTTT-3'
	3'-TAAAT AATTAACAACCAGACTA      ATGGATACAATAAT      TACCAAGACAAGTCCTT CAAAA-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Warning! Primer pair was generated using suboptimal parameters. 
Primer Index: 00X-131557987
Forward Primer: 5'-TTTTGGATGTCAGCTATAGGGTTGGAGAAAATCTT-3' Tm: 59.578
Reverse Primer: 5'-CAACAAATGATGCTAATCTAACTGGATGTCTACATG-3' Tm: 59.498
Expected PCR Product Length: 337
Expected CelI Cleavage Product Sizes: 131, 206
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chrX:131558092-131558139

Rank: 68	Homology Score: 8
	Genomic Region: Intergenic	Closest Gene: Prdm2
Match Type: R19L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-TACCT TTCAGGGTTGTTCTAAA   AACTAACTGGAGAACACAA    ATGGCGCCTGGCATGAA GTAAC-3'
	3'-ATGGA AAGTCCCAACAAGATTT   TTGATTGACCTCTTGTGTT    TACCGCGGACCGTACTT CATTG-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 005-161756246
Forward Primer: 5'-CTCTAGGGGCTAAGTATCTCAGCT-3' Tm: 59.038
Reverse Primer: 5'-GGTGGCAAAATCTAAACCCTGTGG-3' Tm: 59.038
Expected PCR Product Length: 280
Expected CelI Cleavage Product Sizes: 130, 150
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr5:161756348-161756400

Rank: 69	Homology Score: 8
	Genomic Region: Intergenic	Closest Gene: Far1
Match Type: R13L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-CTGTC TTAATTGTTGTTGTAGT      ACGGAGACTAACC       CTGAATGTGGGCAGGAA TATTC-3'
	3'-GACAG AATTAACAACAACATCA      TGCCTCTGATTGG       GACTTACACCCGTCCTT ATAAG-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 001-171517384
Forward Primer: 5'-CACCTTGTGTTTCCAGAACTCTGC-3' Tm: 59.038
Reverse Primer: 5'-CTAGTCCTATCACATCCACGGTC-3' Tm: 59.148
Expected PCR Product Length: 292
Expected CelI Cleavage Product Sizes: 157, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr1:171517516-171517562

Rank: 70	Homology Score: 8
	Genomic Region: Intergenic	Closest Gene: Sfrp2
Match Type: L13R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-TCATG TACCAGCCCAGCTGCAG      TCCAGCTTCTATG       TCCAGCACTGCACTGAA ACTGT-3'
	3'-AGTAC ATGGTCGGGTCGACGTC      AGGTCGAAGATAC       AGGTCGTGACGTGACTT TGACA-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 002-175409656
Forward Primer: 5'-CATCGGGCAGGGTTTCAAAGC-3' Tm: 59.378
Reverse Primer: 5'-GCCCAAGCTGACATTTGAGAGAC-3' Tm: 59.148
Expected PCR Product Length: 303
Expected CelI Cleavage Product Sizes: 159, 144
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr2:175409790-175409836

Rank: 71	Homology Score: 8
	Genomic Region: Intergenic	Closest Gene: Rgs10
Match Type: L22R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-AATTT TCTCTGCCCACCTCCGT  TGAAAGCATCCCCCCAAGGCTG  ATCACAAATACACTGAA GCTAA-3'
	3'-TTAAA AGAGACGGGTGGAGGCA  ACTTTCGTAGGGGGGTTCCGAC  TAGTGTTTATGTGACTT CGATT-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 001-187665749
Forward Primer: 5'-GGAGGGAAGGAGGGAAGGAA-3' Tm: 59.508
Reverse Primer: 5'-ACACCAGTCTTGCTGAGGGC-3' Tm: 59.508
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 132, 138
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr1:187665851-187665906

Rank: 72	Homology Score: 8
	Genomic Region: Intergenic	Closest Gene: Pik3ap1
Match Type: R16L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-GGCTT TTTTGTGTTGTACTAGT     TCATATCTGCAAACCA     CTGGAGCACAGCAGGAA ACAGA-3'
	3'-CCGAA AAAACACAACATGATCA     AGTATAGACGTTTGGT     GACCTCGTGTCGTCCTT TGTCT-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 001-246639403
Forward Primer: 5'-GCCCATGCGAATGGGTGGAA-3' Tm: 59.508
Reverse Primer: 5'-TCAAAAGCAGCCTCCTGATGTGTC-3' Tm: 59.038
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 134, 136
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr1:246639510-246639559

Rank: 73	Homology Score: 8
	Genomic Region: Intergenic	Closest Gene: Mir100
Match Type: L12R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-AGTAC TTCAAGACCAGCTCCTT       TGAATCGTTCTC       CCCAGAACAAGGCTGCA CCTGA-3'
	3'-TCATG AAGTTCTGGTCGAGGAA       ACTTAGCAAGAG       GGGTCTTGTTCCGACGT GGACT-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 008-044521883
Forward Primer: 5'-CAGAGGACCTGTGAGAGACAGT-3' Tm: 59.258
Reverse Primer: 5'-GAAGCAAATGTGAGAGGAAAAGCCTTGATG-3' Tm: 60.058
Expected PCR Product Length: 439
Expected CelI Cleavage Product Sizes: 308, 131
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr8:44522166-44522211

Rank: 74	Homology Score: 8
	Genomic Region: Intergenic	Closest Gene: Epha7
Match Type: R18L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-AAAAG TTCAGTGTGCTCCTGGG    TATATGGTTGGCAGTACA    ATGGAGCTCTGCAGACA GTATG-3'
	3'-TTTTC AAGTCACACGAGGACCC    ATATACCAACCGTCATGT    TACCTCGAGACGTCTGT CATAC-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 005-045632950
Forward Primer: 5'-CCAGAGACTGCCACACTTGGA-3' Tm: 59.378
Reverse Primer: 5'-CCTGCCATCTCTCTGCATATCTTC-3' Tm: 59.038
Expected PCR Product Length: 322
Expected CelI Cleavage Product Sizes: 172, 150
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr5:45633094-45633145

Rank: 75	Homology Score: 8
	Genomic Region: Intergenic	Closest Gene: Stk17b
Match Type: R21L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-TGGCA TTCCGTTTGGTCCTGGT  TACTCCTGCTGACTCATTAGA   ATTGAGCTGGCTGGGAA GGTAA-3'
	3'-ACCGT AAGGCAAACCAGGACCA  ATGAGGACGACTGAGTAATCT   TAACTCGACCGACCCTT CCATT-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 009-052013154
Forward Primer: 5'-GAGGCTGAGCTTGGCTTGCTT-3' Tm: 59.378
Reverse Primer: 5'-GCAAGGTCACATAGTGTCACTTGG-3' Tm: 59.038
Expected PCR Product Length: 311
Expected CelI Cleavage Product Sizes: 157, 154
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr9:52013282-52013336

Rank: 76	Homology Score: 8
	Genomic Region: Intergenic	Closest Gene: Tcf12
Match Type: L24R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-TGGAG TGCCTGCCCTGCTCTAG ATCAGTAAGGCTCTAGGATCAGTC TCCAGCACCACACAGAA CTTGA-3'
	3'-ACCTC ACGGACGGGACGAGATC TAGTCATTCCGAGATCCTAGTCAG AGGTCGTGGTGTGTCTT GAACT-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. Warning! The sequence of this region of the genome is poorly mapped. 
Primer Index: 008-076531196
Forward Primer: 5'-GGCTAGAAGAGGAGATATGGGG-3' Tm: 59.258
Reverse Primer: 5'-CCTAGGCTGACCTTGAACTCATTG-3' Tm: 59.038
Expected PCR Product Length: 353
Expected CelI Cleavage Product Sizes: 217, 136
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr8:76531382-76531439

Rank: 77	Homology Score: 8
	Genomic Region: Intergenic	Closest Gene: Clec14a
Match Type: L17R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-AGGTC TTGCTGCCCATCTTCAA    TAAGCCAAGAGAAAAGA     ACCAGCTTAACAGTGAA AAGTA-3'
	3'-TCCAG AACGACGGGTAGAAGTT    ATTCGGTTCTCTTTTCT     TGGTCGAATTGTCACTT TTCAT-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 006-079162173
Forward Primer: 5'-ACACAGAAGGCTCTCCAGAGAC-3' Tm: 59.258
Reverse Primer: 5'-ACTTTGATCTCACTTCAGGTCCCC-3' Tm: 59.038
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 138, 132
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr6:79162284-79162334

Rank: 78	Homology Score: 8
	Genomic Region: Intergenic	Closest Gene: Rps29
Match Type: L16R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-GCACA TTCCTCCACATTTCCAT     CTCGAGGAAAGATTGG     ATCAGATCAAGAGTGAA ACTCA-3'
	3'-CGTGT AAGGAGGTGTAAAGGTA     GAGCTCCTTTCTAACC     TAGTCTAGTTCTCACTT TGAGT-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 006-090557385
Forward Primer: 5'-GTCCCCACAGGCATATGATCG-3' Tm: 59.378
Reverse Primer: 5'-GTGCTTCGGGCAGTAGAGCT-3' Tm: 59.508
Expected PCR Product Length: 348
Expected CelI Cleavage Product Sizes: 217, 131
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr6:90557575-90557624

Rank: 79	Homology Score: 5
	Genomic Region: Exon	Closest Gene: Hsd17b6
Match Type: R14L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-ACTTC TTCAGTTCTCGTCTGGT      GGTGTTGCAAACTG      AAGGAGCAGAGGAGGGA GGTCA-3'
	3'-TGAAG AAGTCAAGAGCAGACCA      CCACAACGTTTGAC      TTCCTCGTCTCCTCCCT CCAGT-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 007-001118581
Forward Primer: 5'-GAAGAAAAGTCTGGCATCCCAGC-3' Tm: 59.148
Reverse Primer: 5'-GCCTGGACTCTTGGTCTTCCT-3' Tm: 59.378
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 135, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr7:1118690-1118737

Rank: 80	Homology Score: 5
	Genomic Region: Exon	Closest Gene: Atrn
Match Type: L24R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-CACCT TTCCTCTCCTGCTTCAT ACCCTGCAGTTCTCCTATTATCTG ACTAGAACAGCCCTCTA GGCAA-3'
	3'-GTGGA AAGGAGAGGACGAAGTA TGGGACGTCAAGAGGATAATAGAC TGATCTTGTCGGGAGAT CCGTT-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 003-118698225
Forward Primer: 5'-GTTAAGAGGGGTGAGCAGTCC-3' Tm: 59.378
Reverse Primer: 5'-CTCAGAGAAAGGCTCCCTCTTCA-3' Tm: 59.148
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 137, 133
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr3:118698331-118698388

Rank: 81	Homology Score: 5
	Genomic Region: Exon	Closest Gene: Hsd17b6
Match Type: R14L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-ACTTC TTCAGTTCTCGTCTGGT      GGTGTTGCAAACTG      AAGGAGCAGAGGAGGGA GGTCA-3'
	3'-TGAAG AAGTCAAGAGCAGACCA      CCACAACGTTTGAC      TTCCTCGTCTCCTCCCT CCAGT-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 007-001268176
Forward Primer: 5'-GAAGAAAAGTCTGGCATCCCAGC-3' Tm: 59.148
Reverse Primer: 5'-GCCTGGACTCTTGGTCTTCCT-3' Tm: 59.378
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 135, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr7:1268285-1268332

Rank: 82	Homology Score: 5
	Genomic Region: Exon	Closest Gene: Cxcl10
Match Type: L13R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-CTACA TTCGAGCCCAGCCACAT      CCCGAGCCAACCT       TCCAGAAGCACCATGAA CCCAA-3'
	3'-GATGT AAGCTCGGGTCGGTGTA      GGGCTCGGTTGGA       AGGTCTTCGTGGTACTT GGGTT-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 014-017265922
Forward Primer: 5'-CAGGGAGGGAAACTCTGTGC-3' Tm: 59.508
Reverse Primer: 5'-GAGCTCAGTCCTCTTTCTGACTCT-3' Tm: 59.038
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 139, 131
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr14:17266036-17266082

Rank: 83	Homology Score: 5
	Genomic Region: Exon	Closest Gene: Ube2q1
Match Type: R16L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-TCTTC TACAGGTATGTTCTGGG     CGGAGGTGCCATCTGC     ATGGAGCTGCTCACCAA GCAGG-3'
	3'-AGAAG ATGTCCATACAAGACCC     GCCTCCACGGTAGACG     TACCTCGACGAGTGGTT CGTCC-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 002-181986166
Forward Primer: 5'-AGTCCTCTCTGGAGGGTGAGT-3' Tm: 59.378
Reverse Primer: 5'-GAAAGGGCCATATTATGGCTGCAG-3' Tm: 59.038
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 135, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr2:181986274-181986323

Rank: 84	Homology Score: 5
	Genomic Region: Exon	Closest Gene: Rap1gds1
Match Type: L14R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-CACCG TGCTTTCCCAGCACCAA      AAAATAAACCAGAA      ACCAAAACAACAACAAA AACAA-3'
	3'-GTGGC ACGAAAGGGTCGTGGTT      TTTTATTTGGTCTT      TGGTTTTGTTGTTGTTT TTGTT-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 002-236523497
Forward Primer: 5'-GGGAGAAATCGCAGGGAAAGAATG-3' Tm: 59.038
Reverse Primer: 5'-CTCCTCACACGCAGTAACCTACA-3' Tm: 59.148
Expected PCR Product Length: 287
Expected CelI Cleavage Product Sizes: 141, 146
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr2:236523612-236523659

Rank: 85	Homology Score: 5
	Genomic Region: Exon	Closest Gene: Pcdhb8
Match Type: R20L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-GTGTC TTCACTTTTTCTCTTGT   CTGTGCTGCTGTTCGTGGGC   GTGAGGCTGTGCAGGAA GGCCA-3'
	3'-CACAG AAGTGAAAAAGAGAACA   GACACGACGACAAGCACCCG   CACTCCGACACGTCCTT CCGGT-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 018-030171759
Forward Primer: 5'-CTTCTCTCAGCCCTACCTGC-3' Tm: 59.508
Reverse Primer: 5'-GAGTCTTCAGTCAGACAGACCTCA-3' Tm: 59.038
Expected PCR Product Length: 285
Expected CelI Cleavage Product Sizes: 131, 154
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr18:30171861-30171914

Rank: 86	Homology Score: 5
	Genomic Region: Exon	Closest Gene: LOC499573
Match Type: R18L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-GCTGC TCCAGCGTTGTTCTCAT    TGGCTCTGGGAGCACTCG    ATGGACCAGAGAAGTAA CTTCC-3'
	3'-CGACG AGGTCGCAACAAGAGTA    ACCGAGACCCTCGTGAGC    TACCTGGTCTCTTCATT GAAGG-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 002-094928649
Forward Primer: 5'-GCTGAAGCCTGAGATGTTGTGC-3' Tm: 59.258
Reverse Primer: 5'-ACCTTGCCTGGTTCGACAGAC-3' Tm: 59.378
Expected PCR Product Length: 282
Expected CelI Cleavage Product Sizes: 152, 130
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr2:94928773-94928824

Rank: 87	Homology Score: 5
	Genomic Region: Promoter	Closest Gene: Mettl4
Match Type: L23R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-TGATC TACCTGTCGAGCTCCAG AGCAACCTAGTGAGACCTTGTCT  ACAAAAACAAAACAAAA CAAAA-3'
	3'-ACTAG ATGGACAGCTCGAGGTC TCGTTGGATCACTCTGGAACAGA  TGTTTTTGTTTTGTTTT GTTTT-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 009-110735185
Forward Primer: 5'-CCTCCCCCCCAAAAAAACCAAAC-3' Tm: 59.148
Reverse Primer: 5'-GTGGAAACCTTAAGAGGGCACAG-3' Tm: 59.148
Expected PCR Product Length: 349
Expected CelI Cleavage Product Sizes: 197, 152
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr9:110735352-110735408

Rank: 88	Homology Score: 5
	Genomic Region: Promoter	Closest Gene: Mir153
Match Type: L20R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-GCTCC TTCCTGCCCACCCTCAG   GGTCTAGCCACTAGACTCTT   CCCAGTACAGACCTGAA GATAC-3'
	3'-CGAGG AAGGACGGGTGGGAGTC   CCAGATCGGTGATCTGAGAA   GGGTCATGTCTGGACTT CTATG-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 006-144519986
Forward Primer: 5'-CTCTGCCTTTCAAGTGCTGGG-3' Tm: 59.378
Reverse Primer: 5'-TGCAAACCCCTTGGATGCGG-3' Tm: 59.508
Expected PCR Product Length: 328
Expected CelI Cleavage Product Sizes: 198, 130
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr6:144520155-144520208

Rank: 89	Homology Score: 5
	Genomic Region: Intron	Closest Gene: Gas2
Match Type: R21L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-GGGTA TATCTTGTTGTTCTGGC  CATTGTTATGGGTCATAGGTT   TTATAGCTGGGTAGGAA ATGTA-3'
	3'-CCCAT ATAGAACAACAAGACCG  GTAACAATACCCAGTATCCAA   AATATCGACCCATCCTT TACAT-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 001-101789928
Forward Primer: 5'-GTTTCTCCTCACCAGGATGGATAG-3' Tm: 59.038
Reverse Primer: 5'-CCAAGACCAGTATTCCACTTCTCC-3' Tm: 59.038
Expected PCR Product Length: 444
Expected CelI Cleavage Product Sizes: 146, 298
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr1:101790045-101790099

Rank: 90	Homology Score: 5
	Genomic Region: Intron	Closest Gene: Abcc4
Match Type: L12R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-AAACG TTCCTTGCTAACTCCTT       TCTTTTCACGGG       GCCAGAATAACTCTTAA ATGTT-3'
	3'-TTTGC AAGGAACGATTGAGGAA       AGAAAAGTGCCC       CGGTCTTATTGAGAATT TACAA-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 015-103441033
Forward Primer: 5'-GCTGGGATGACCTGATATGATCTC-3' Tm: 59.038
Reverse Primer: 5'-CCCTCAGCATAGAATATCACCAGC-3' Tm: 59.038
Expected PCR Product Length: 334
Expected CelI Cleavage Product Sizes: 200, 134
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr15:103441208-103441253

Rank: 91	Homology Score: 5
	Genomic Region: Intron	Closest Gene: Commd1
Match Type: L15R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-TCGGC TACCTCTCCAGCACCAT     GTCTGCCTACATCCC      ACCATACTAACAATGGA CTAAA-3'
	3'-AGCCG ATGGAGAGGTCGTGGTA     CAGACGGATGTAGGG      TGGTATGATTGTTACCT GATTT-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 014-103592966
Forward Primer: 5'-TACAGGAGGAGTGGCCTTGTG-3' Tm: 59.378
Reverse Primer: 5'-GCGTCAAGGTAAAATGACCAAGGG-3' Tm: 59.038
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 133, 137
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr14:103593073-103593121

Rank: 92	Homology Score: 5
	Genomic Region: Intron	Closest Gene: Elmod3
Match Type: L17R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-GTACT TCCATGTCCAGCTCCCC    AGCAGGCATGGTTCTAA     ACCAGCAACAGACTGAA TCGCT-3'
	3'-CATGA AGGTACAGGTCGAGGGG    TCGTCCGTACCAAGATT     TGGTCGTTGTCTGACTT AGCGA-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. Warning! The sequence of this region of the genome is poorly mapped. 
Primer Index: 004-105993532
Forward Primer: 5'-GGGGCGGGCAGTAAGAACAT-3' Tm: 59.508
Reverse Primer: 5'-GGTTCAGTGTCAGGCTATGATGG-3' Tm: 59.148
Expected PCR Product Length: 308
Expected CelI Cleavage Product Sizes: 164, 144
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr4:105993669-105993719

Rank: 93	Homology Score: 5
	Genomic Region: Intron	Closest Gene: Ephb1
Match Type: L14R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-GAGCC TTCCAGCCATGCTCCAG      TTCTGTCATTTTCC      ATCAGAAAAACATCCAA TGCCT-3'
	3'-CTCGG AAGGTCGGTACGAGGTC      AAGACAGTAAAAGG      TAGTCTTTTTGTAGGTT ACGGA-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 008-106942889
Forward Primer: 5'-GATCAGCAGGAGCAGGTCATCT-3' Tm: 59.258
Reverse Primer: 5'-CTTATTCCCTCCTCCACCCC-3' Tm: 59.508
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 135, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr8:106942998-106943045

Rank: 94	Homology Score: 5
	Genomic Region: Intron	Closest Gene: Dlgap1
Match Type: L12R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-TTACT TTCCCAAACAGCTCCGT       GGAGACAAAGTA       ACCAGAACAGCAGAAAA CAAAA-3'
	3'-AATGA AAGGGTTTGTCGAGGCA       CCTCTGTTTCAT       TGGTCTTGTCGTCTTTT GTTTT-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 009-109595283
Forward Primer: 5'-GTGAAGCAGGAGCGCAGATG-3' Tm: 59.508
Reverse Primer: 5'-CCCTCAGACTGAACGTCTAAGC-3' Tm: 59.258
Expected PCR Product Length: 368
Expected CelI Cleavage Product Sizes: 131, 237
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr9:109595389-109595434

Rank: 95	Homology Score: 5
	Genomic Region: Intron	Closest Gene: Atp10a
Match Type: R18L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-TCTTC TTAAGTCTAGTGGTGGT    TGGTATACTGTGGCCATG    AAGGGGGTGGGCAGGCA GGTCA-3'
	3'-AGAAG AATTCAGATCACCACCA    ACCATATGACACCGGTAC    TTCCCCCACCCGTCCGT CCAGT-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 001-110232556
Forward Primer: 5'-GACGTTGATCCATAATCCTCACCATAGTAC-3' Tm: 60.058
Reverse Primer: 5'-CACGCATGCACACACACACAAAC-3' Tm: 59.148
Expected PCR Product Length: 387
Expected CelI Cleavage Product Sizes: 191, 196
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr1:110232719-110232770

Rank: 96	Homology Score: 5
	Genomic Region: Intron	Closest Gene: Trappc9
Match Type: R20L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-AGACT TTCATTGTTCACCTGGA   ACCACATCTCTTCTCTAACC   CAGGAGCAGGACAGGAA AAGTC-3'
	3'-TCTGA AAGTAACAAGTGGACCT   TGGTGTAGAGAAGAGATTGG   GTCCTCGTCCTGTCCTT TTCAG-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 007-110768688
Forward Primer: 5'-TGACCAGGAGTCTTGCTGAAGCT-3' Tm: 59.148
Reverse Primer: 5'-GGCTCTGGTCCCTTATAGATGATG-3' Tm: 59.038
Expected PCR Product Length: 285
Expected CelI Cleavage Product Sizes: 131, 154
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr7:110768790-110768843

Rank: 97	Homology Score: 5
	Genomic Region: Intron	Closest Gene: Nlgn1
Match Type: R23L
	          Right Nuclease
	         TTCAGTGTTGTTCTGGT
	5'-TTGTT TTCATTTTTGTTTTCTT ATTTTTGCTGTTGAGGAAAGGCC  ATGAAGCTGGGTGAGAA GGGAG-3'
	3'-AACAA AAGTAAAAACAAAAGAA TAAAAACGACAACTCCTTTCCGG  TACTTCGACCCACTCTT CCCTC-5'
	                                                    TACCTCGACCCGTCCTT
	                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 002-111467723
Forward Primer: 5'-CCTGAGTCCAGGAAGAGTTGGT-3' Tm: 59.258
Reverse Primer: 5'-CCTCACGGACATGGTATTTGCATC-3' Tm: 59.038
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 131, 139
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr2:111467824-111467880

Rank: 98	Homology Score: 5
	Genomic Region: Intron	Closest Gene: Adck1
Match Type: L13R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-TGGTG TTCCTGGCCAGCAAGAC      AACCCCGTGGGGT       ACCAGGACCAGACAGAA CACAG-3'
	3'-ACCAC AAGGACCGGTCGTTCTG      TTGGGGCACCCCA       TGGTCCTGGTCTGTCTT GTGTC-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 006-111938978
Forward Primer: 5'-AGGGGCACGTGGAGGATGAT-3' Tm: 59.508
Reverse Primer: 5'-TGCAGGGGACACTTTGTCCTAC-3' Tm: 59.258
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 134, 136
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr6:111939087-111939133

Rank: 99	Homology Score: 5
	Genomic Region: Intron	Closest Gene: Tmem103
Match Type: L22R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-CTGAG TTCCAGGCCAGCCTGAT  CTACAGAGTAAGTTCCAAGATA  ACCAGGGCTACACAGAA AAACC-3'
	3'-GACTC AAGGTCCGGTCGGACTA  GATGTCTCATTCAAGGTTCTAT  TGGTCCCGATGTGTCTT TTTGG-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 008-114686715
Forward Primer: 5'-CTGGTGTGTGTGAAGACAGCAAG-3' Tm: 59.148
Reverse Primer: 5'-GAGATAGCCTCAGGGAGCAC-3' Tm: 59.508
Expected PCR Product Length: 295
Expected CelI Cleavage Product Sizes: 165, 130
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr8:114686850-114686905

Rank: 100	Homology Score: 5
	Genomic Region: Intron	Closest Gene: RGD1305733
Match Type: L21R
	           Left Nuclease
	         TTCCTGCCCAGCTCCAT
	5'-GTCAG TCCCTGCCTAGCCCCAA  CCCTTTGGTTTTGCCTCTTTG   ACCATGACAACTTTGCA TACTC-3'
	3'-CAGTC AGGGACGGATCGGGGTT  GGGAAACCAAAACGGAGAAAC   TGGTACTGTTGAAACGT ATGAG-5'
	                                                    TGGTCTTGTTGTGACTT
	                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 010-000118878
Forward Primer: 5'-CCCAGGTATTCTGCCATAGTAGC-3' Tm: 59.148
Reverse Primer: 5'-CCACTGGTGTCCTCTGAATTTCC-3' Tm: 59.148
Expected PCR Product Length: 296
Expected CelI Cleavage Product Sizes: 134, 162
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr10:118983-119037

Plate format Oligo Order Form
Well
Name
Sequence
5' mods
Internal
Notes
Primer Plate #1
A1
006-138443970F
GGAATCGGCACATGCAGATCTTTG
Tesson_IgM_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
A2
006-138443970R
TCTGGTCCTCAGAGAGTCAGTC
Tesson_IgM_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
B1
001-252428669F
GTTGTGTTTCAGGACAGCACTACC
Tesson_IgM_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
B2
001-252428669R
GTTGGAGAGGCCACATTCTTAACC
Tesson_IgM_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
C1
020-016460972F
GTCAAGGACGAAGTCATCTGGAG
Tesson_IgM_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
C2
020-016460972R
GACAAGCTGACTGCTGCTAGAG
Tesson_IgM_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
D1
009-007190270F
AGTTGGGCTGGTAGAAATTGCTGG
Tesson_IgM_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
D2
009-007190270R
CTGAGTGAAACAAACGGCTCCGA
Tesson_IgM_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
E1
015-087079625F
CAATCACTCCCTGCAATCCCC
Tesson_IgM_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
E2
015-087079625R
TGACGCCTCCACTGTCCTAG
Tesson_IgM_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
F1
002-159432579F
GCTAAATCCCCAACCCTGCAG
Tesson_IgM_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
F2
002-159432579R
GTGTTTACTTGCTCAAACACCCGG
Tesson_IgM_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
G1
002-204001007F
AATTTATGGGCTCTGTGCCGTGC
Tesson_IgM_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
G2
002-204001007R
CCCAGTGCTAAGTTGAGTGTGGC
Tesson_IgM_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
H1
003-100952790F
GCCATGAAGAAGACATGCAGGAG
Tesson_IgM_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
H2
003-100952790R
GGATCACTTGAAATGTGGAGCTTCCTAC
Tesson_IgM_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
A3
00X-144712285F
CCAAGCGAGACAGAGATCGG
Tesson_IgM_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
A4
00X-144712285R
GGTACTTGGCCCTCAGAATCG
Tesson_IgM_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
B3
011-031478392F
GATCTGATCCCCTTTTCTGGTGTG
Tesson_IgM_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
B4
011-031478392R
CTTCTTTAGCACGTCGCCCC
Tesson_IgM_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
C3
007-067524783F
TGATCAGAGCCTGCCCCTTC
Tesson_IgM_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
C4
007-067524783R
GACAGGGTGCTTCCTCACAATAC
Tesson_IgM_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
D3
020-012161079F
CCGTTGCCCGAAAAGCACCT
Tesson_IgM_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
D4
020-012161079R
GAACCCATTTGCTCTCAGCCTTG
Tesson_IgM_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
E3
001-138061468F
CCGTGAAGCAGGTCACAAAGC
Tesson_IgM_Ranking-Homology_5T	Primer pair generated using optimal parameters. Warning! The sequence of this region of the genome is poorly mapped. 
E4
001-138061468R
CAGCAAGGGAAGTGCTACTTGATG
Tesson_IgM_Ranking-Homology_5T	Primer pair generated using optimal parameters. Warning! The sequence of this region of the genome is poorly mapped. 
F3
010-013862379F
CATAGGTGGGGCCTTATCAGAG
Tesson_IgM_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
F4
010-013862379R
GGTCCCTAGTCAAGGACATTGTCT
Tesson_IgM_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
G3
004-141883124F
AATCCGCAGTCTAAGCCAACAGAG
Tesson_IgM_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
G4
004-141883124R
TGCTAGGCAAGCACTCTACCAC
Tesson_IgM_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
H3
003-145456877F
GGCGACAGTTCCTCTGTTTTTGC
Tesson_IgM_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
H4
003-145456877R
CACAGTAGTGGCTGTAGGCATTTG
Tesson_IgM_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
A5
012-015035708F
AGCCTCCTGGTTACGTCCCAA
Tesson_IgM_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
A6
012-015035708R
TGACTGCTGTGTGTGGGGAG
Tesson_IgM_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
B5
002-214232497F
CACGTGTGTGCCTCAGTAACAG
Tesson_IgM_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
B6
002-214232497R
AGCAGGCCCTGGAACAGTTCT
Tesson_IgM_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
C5
001-242536006F
TGGAGGTTGGGAGAGTAGAGG
Tesson_IgM_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
C6
001-242536006R
TGAAGGAGGGAACACTCAGCAG
Tesson_IgM_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
D5
005-049320556F
CTGTGGTGAAGGTGTTACTGTGTC
Tesson_IgM_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
D6
005-049320556R
GAGAAGGCTGGACCGAATAGAAG
Tesson_IgM_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
E5
006-075907048F
GGTGTCCTCATTCACCTCTGACAA
Tesson_IgM_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
E6
006-075907048R
GTGTTTGGAACATCCTCTGCGAC
Tesson_IgM_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
F5
014-082098705F
AGCCAAGGAAGAAACCTGTGCAGC
Tesson_IgM_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
F6
014-082098705R
CTGTGGATGGCTAGAGTGTACTCA
Tesson_IgM_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
G5
014-085768096F
GGGAATGGTTAGCCCCATCTC
Tesson_IgM_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
G6
014-085768096R
CATGAGGAAACAGTTCAGGAGGG
Tesson_IgM_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
H5
004-100499585F
GGCATAGCCTCTCAGGAAACAG
Tesson_IgM_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
H6
004-100499585R
CTTCCAGGACAGAACAGGAAAGAC
Tesson_IgM_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
A7
002-106965669F
ATAGTGGCCAGTGCCTGGAC
Tesson_IgM_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
A8
002-106965669R
CCAGGAGAAAAGGCCATCTTCCA
Tesson_IgM_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
B7
005-113632907F
GGAGGAATCTCTAGAAAGTCCCAG
Tesson_IgM_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
B8
005-113632907R
GGCTCCTATCTGCAGGTCCA
Tesson_IgM_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
C7
00X-117110415F
CTTTGCCCACAGAATAGCAGCC
Tesson_IgM_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
C8
00X-117110415R
CTAGCCAAGCGCGACAAAGTTC
Tesson_IgM_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
D7
005-123636672F
CTCTGAGTGACACGAATTAGTGGG
Tesson_IgM_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
D8
005-123636672R
GGAGTGTGTCACTAAGGCTGG
Tesson_IgM_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
E7
001-127719260F
CCAACATTCTCCCAAGAGATTCTGGAAAC
Tesson_IgM_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
E8
001-127719260R
GTTCCAGGTCAACCAGGACTAC
Tesson_IgM_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
F7
00X-133545933F
CGCTCTTCAAGGTATTTTGCCCAC
Tesson_IgM_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
F8
00X-133545933R
CTAGCCAAACACGACAAAGATCGG
Tesson_IgM_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
G7
001-140972716F
GGGACCACCTCAGCTAAGGA
Tesson_IgM_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
G8
001-140972716R
GTGGGGTTGTTGGTTTTCAGCTC
Tesson_IgM_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
H7
00X-146103263F
CTACACTCCTTTTTGTTGGGTGGG
Tesson_IgM_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
H8
00X-146103263R
CTCTGCTGCAGAGCCTTTTTTCC
Tesson_IgM_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
A9
003-150734535F
CACCAGCCAAGAACGGCGTCTTT
Tesson_IgM_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
A10
003-150734535R
CACCTCTGCACCTCCTTCTAAC
Tesson_IgM_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
B9
012-015478690F
CCACTCAAGACAAGCAGAAAGGAC
Tesson_IgM_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
B10
012-015478690R
CAAAACAGCAGCAGCAAAAGCTGG
Tesson_IgM_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
C9
00X-016509931F
CTACCAATGTCTATGGAGTGACCG
Tesson_IgM_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
C10
00X-016509931R
GCCTAGAGTATCCCAAATGTGCAC
Tesson_IgM_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
D9
001-001765937F
TTCTAGCCAAGAGGTCCAAAGGCC
Tesson_IgM_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
D10
001-001765937R
TGCATGGCCTCTGCATCAGC
Tesson_IgM_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
E9
009-019047855F
GTGGCTTTGGTCCTCATCTGTC
Tesson_IgM_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
E10
009-019047855R
CCTCTTTCGGAGATAGGCATGTTC
Tesson_IgM_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
F9
0Un-021787610F
CCTGGGATGTGGTGTAAGTTGG
Tesson_IgM_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
F10
0Un-021787610R
GAGGAGCCAAATCTGGACCATTAG
Tesson_IgM_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
G9
013-002441765F
CTGTCTCTGTCTCTCTTAGTTCCC
Tesson_IgM_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
G10
013-002441765R
CCCTTTTTGCCTTTCCATGCAGTG
Tesson_IgM_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
H9
007-025308852F
CCAGTCTGATGGTTATACATACAGTGCAGT
Tesson_IgM_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
H10
007-025308852R
GGTTTTCAACCTGAGTGGGAGAAG
Tesson_IgM_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
A11
00X-027254372F
CCATTATCGCTAGCCTAGCTTACG
Tesson_IgM_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
A12
00X-027254372R
GACTGGGAATAAGTGGTGTGGTAC
Tesson_IgM_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
B11
011-028431745F
GCTCAGAACAGCTAATGTGAGTGC
Tesson_IgM_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
B12
011-028431745R
CAATGACTGTCCAGAACTGAGGC
Tesson_IgM_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
C11
012-031781347F
CGCGTAATACGCCTTTTGTTGACC
Tesson_IgM_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
C12
012-031781347R
GAGCAGTGAACAGCATTAGCTTCC
Tesson_IgM_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
D11
dom-003376644F
CCTGGGATGTGGTGTAAGTTGG
Tesson_IgM_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
D12
dom-003376644R
GAGGAGCCAAATCTGGACCATTAG
Tesson_IgM_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
E11
004-003843423F
GGTGACCAATACCAACCATCAGG
Tesson_IgM_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
E12
004-003843423R
CCTCCTTTCAGGAAGGCTCTTTCT
Tesson_IgM_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
F11
0Un-038608458F
CCTGGGATGTGGTGTAAGTTGG
Tesson_IgM_Ranking-Homology_5T	Primer pair generated using optimal parameters. Warning! The sequence of this region of the genome is poorly mapped. 
F12
0Un-038608458R
GAGACAACATACCCTGCTCTCAC
Tesson_IgM_Ranking-Homology_5T	Primer pair generated using optimal parameters. Warning! The sequence of this region of the genome is poorly mapped. 
G11
014-039438747F
GCTCCATAAACTGGGCCTGAGA
Tesson_IgM_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
G12
014-039438747R
GCTAGCCTCTACCTCTTCCTG
Tesson_IgM_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
H11
017-041218534F
CCAGGCCATGATTATTCTGCAGG
Tesson_IgM_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
H12
017-041218534R
TCCCAAGTCACCTGCAAGCC
Tesson_IgM_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
Primer Plate #2
A1
014-042588777F
GAGACTACAGATGCCAAAAGTTTCCATACC
Tesson_IgM_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
A2
014-042588777R
ACACACACACACACACACACACAC
Tesson_IgM_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
B1
009-004673112F
AATCCACCGAGCCTAGTAACTGC
Tesson_IgM_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
B2
009-004673112R
TACACTGCGCTCACACAGCAAG
Tesson_IgM_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
C1
0Un-051334472F
CCTGGGATGTGGTGTAAGTTGG
Tesson_IgM_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
C2
0Un-051334472R
GAGGAGCCAAATCTGGACCATTAG
Tesson_IgM_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
D1
0Un-051940753F
CAAACAGAGCCAGGTGTGGTG
Tesson_IgM_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
D2
0Un-051940753R
GCCCTCTGTGTCATGCCTTATTAC
Tesson_IgM_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
E1
019-057896612F
CCCAGTGCTGGGGTTACAAACA
Tesson_IgM_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
E2
019-057896612R
TCTTAGCCAGGAATGAGGCTGC
Tesson_IgM_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
F1
003-060396362F
CCACTGGGAGTGTATTCGGTATG
Tesson_IgM_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
F2
003-060396362R
GTAAACAGGCCTCTACAGAACGG
Tesson_IgM_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
G1
0Un-061148009F
CCTGGGATGTGGTGTAAGTTGG
Tesson_IgM_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
G2
0Un-061148009R
GAGACAACATACCCTGCTCTCAC
Tesson_IgM_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
H1
009-065106893F
CCTCTGTTGGATGTGGAGTTGG
Tesson_IgM_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
H2
009-065106893R
CGCACACCTATGGACATCTGAG
Tesson_IgM_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
A3
0Un-075352159F
CTTGAGAGACTGTGGTGAATGAGG
Tesson_IgM_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
A4
0Un-075352159R
GGAGGAAAAAGGGGACTGTGTTCA
Tesson_IgM_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
B3
004-092024652F
CTTGAGAGACTGTGGTGAATGAGG
Tesson_IgM_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
B4
004-092024652R
GGAGGAAAAAGGGGACTGTGTTCA
Tesson_IgM_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
C3
00X-093585607F
CCAAGCGCGACAAAGATCGG
Tesson_IgM_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
C4
00X-093585607R
GGTACTTTGCCCACAGAATAGCAG
Tesson_IgM_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
D3
010-055247764F
TGCTACAAGCCAGAGAAGAGCAG
Tesson_IgM_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
D4
010-055247764R
AGACGTCCCTGGTCCATAGC
Tesson_IgM_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
E3
007-112677493F
TAGGGCAGAGCTGATGGACC
Tesson_IgM_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
E4
007-112677493R
CGAGTCTCTTCTTGACTGTTTCCC
Tesson_IgM_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
F3
001-174487424F
GGCCTTAAATGGAATGCATGCTGG
Tesson_IgM_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
F4
001-174487424R
CATCCCTGGGATGAAACCTACTTG
Tesson_IgM_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
G3
004-054569528F
CTGTGAGCAAGCCAACAGATAGC
Tesson_IgM_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
G4
004-054569528R
CCTTGGAGTTGCTACAGGCTTTG
Tesson_IgM_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
H3
008-068387105F
CCGACTAGCTTCTCCCATGAC
Tesson_IgM_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
H4
008-068387105R
TTACTGTCGGTGTCACAGTGCC
Tesson_IgM_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
A5
019-000783634F
CTCCCCCAAGACACTTTGTCTTAC
Tesson_IgM_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
A6
019-000783634R
AAGTATGTGAGAATGCTGCCCAGG
Tesson_IgM_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
B5
003-124119953F
CAGTAGCTGCCTTTGACCAGGA
Tesson_IgM_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
B6
003-124119953R
CAAACCAGACAATGGCTTCTGCTC
Tesson_IgM_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
C5
00X-131557987F
TTTTGGATGTCAGCTATAGGGTTGGAGAAAATCTT
Tesson_IgM_Ranking-Homology_5T	Warning! Primer pair was generated using suboptimal parameters. 
C6
00X-131557987R
CAACAAATGATGCTAATCTAACTGGATGTCTACATG
Tesson_IgM_Ranking-Homology_5T	Warning! Primer pair was generated using suboptimal parameters. 
D5
005-161756246F
CTCTAGGGGCTAAGTATCTCAGCT
Tesson_IgM_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
D6
005-161756246R
GGTGGCAAAATCTAAACCCTGTGG
Tesson_IgM_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
E5
001-171517384F
CACCTTGTGTTTCCAGAACTCTGC
Tesson_IgM_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
E6
001-171517384R
CTAGTCCTATCACATCCACGGTC
Tesson_IgM_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
F5
002-175409656F
CATCGGGCAGGGTTTCAAAGC
Tesson_IgM_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
F6
002-175409656R
GCCCAAGCTGACATTTGAGAGAC
Tesson_IgM_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
G5
001-187665749F
GGAGGGAAGGAGGGAAGGAA
Tesson_IgM_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
G6
001-187665749R
ACACCAGTCTTGCTGAGGGC
Tesson_IgM_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
H5
001-246639403F
GCCCATGCGAATGGGTGGAA
Tesson_IgM_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
H6
001-246639403R
TCAAAAGCAGCCTCCTGATGTGTC
Tesson_IgM_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
A7
008-044521883F
CAGAGGACCTGTGAGAGACAGT
Tesson_IgM_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
A8
008-044521883R
GAAGCAAATGTGAGAGGAAAAGCCTTGATG
Tesson_IgM_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
B7
005-045632950F
CCAGAGACTGCCACACTTGGA
Tesson_IgM_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
B8
005-045632950R
CCTGCCATCTCTCTGCATATCTTC
Tesson_IgM_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
C7
009-052013154F
GAGGCTGAGCTTGGCTTGCTT
Tesson_IgM_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
C8
009-052013154R
GCAAGGTCACATAGTGTCACTTGG
Tesson_IgM_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
D7
008-076531196F
GGCTAGAAGAGGAGATATGGGG
Tesson_IgM_Ranking-Homology_5T	Primer pair generated using optimal parameters. Warning! The sequence of this region of the genome is poorly mapped. 
D8
008-076531196R
CCTAGGCTGACCTTGAACTCATTG
Tesson_IgM_Ranking-Homology_5T	Primer pair generated using optimal parameters. Warning! The sequence of this region of the genome is poorly mapped. 
E7
006-079162173F
ACACAGAAGGCTCTCCAGAGAC
Tesson_IgM_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
E8
006-079162173R
ACTTTGATCTCACTTCAGGTCCCC
Tesson_IgM_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
F7
006-090557385F
GTCCCCACAGGCATATGATCG
Tesson_IgM_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
F8
006-090557385R
GTGCTTCGGGCAGTAGAGCT
Tesson_IgM_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
G7
007-001118581F
GAAGAAAAGTCTGGCATCCCAGC
Tesson_IgM_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
G8
007-001118581R
GCCTGGACTCTTGGTCTTCCT
Tesson_IgM_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
H7
003-118698225F
GTTAAGAGGGGTGAGCAGTCC
Tesson_IgM_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
H8
003-118698225R
CTCAGAGAAAGGCTCCCTCTTCA
Tesson_IgM_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
A9
007-001268176F
GAAGAAAAGTCTGGCATCCCAGC
Tesson_IgM_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
A10
007-001268176R
GCCTGGACTCTTGGTCTTCCT
Tesson_IgM_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
B9
014-017265922F
CAGGGAGGGAAACTCTGTGC
Tesson_IgM_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
B10
014-017265922R
GAGCTCAGTCCTCTTTCTGACTCT
Tesson_IgM_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
C9
002-181986166F
AGTCCTCTCTGGAGGGTGAGT
Tesson_IgM_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
C10
002-181986166R
GAAAGGGCCATATTATGGCTGCAG
Tesson_IgM_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
D9
002-236523497F
GGGAGAAATCGCAGGGAAAGAATG
Tesson_IgM_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
D10
002-236523497R
CTCCTCACACGCAGTAACCTACA
Tesson_IgM_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
E9
018-030171759F
CTTCTCTCAGCCCTACCTGC
Tesson_IgM_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
E10
018-030171759R
GAGTCTTCAGTCAGACAGACCTCA
Tesson_IgM_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
F9
002-094928649F
GCTGAAGCCTGAGATGTTGTGC
Tesson_IgM_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
F10
002-094928649R
ACCTTGCCTGGTTCGACAGAC
Tesson_IgM_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
G9
009-110735185F
CCTCCCCCCCAAAAAAACCAAAC
Tesson_IgM_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
G10
009-110735185R
GTGGAAACCTTAAGAGGGCACAG
Tesson_IgM_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
H9
006-144519986F
CTCTGCCTTTCAAGTGCTGGG
Tesson_IgM_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
H10
006-144519986R
TGCAAACCCCTTGGATGCGG
Tesson_IgM_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
A11
001-101789928F
GTTTCTCCTCACCAGGATGGATAG
Tesson_IgM_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
A12
001-101789928R
CCAAGACCAGTATTCCACTTCTCC
Tesson_IgM_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
B11
015-103441033F
GCTGGGATGACCTGATATGATCTC
Tesson_IgM_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
B12
015-103441033R
CCCTCAGCATAGAATATCACCAGC
Tesson_IgM_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
C11
014-103592966F
TACAGGAGGAGTGGCCTTGTG
Tesson_IgM_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
C12
014-103592966R
GCGTCAAGGTAAAATGACCAAGGG
Tesson_IgM_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
D11
004-105993532F
GGGGCGGGCAGTAAGAACAT
Tesson_IgM_Ranking-Homology_5T	Primer pair generated using optimal parameters. Warning! The sequence of this region of the genome is poorly mapped. 
D12
004-105993532R
GGTTCAGTGTCAGGCTATGATGG
Tesson_IgM_Ranking-Homology_5T	Primer pair generated using optimal parameters. Warning! The sequence of this region of the genome is poorly mapped. 
E11
008-106942889F
GATCAGCAGGAGCAGGTCATCT
Tesson_IgM_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
E12
008-106942889R
CTTATTCCCTCCTCCACCCC
Tesson_IgM_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
F11
009-109595283F
GTGAAGCAGGAGCGCAGATG
Tesson_IgM_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
F12
009-109595283R
CCCTCAGACTGAACGTCTAAGC
Tesson_IgM_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
G11
001-110232556F
GACGTTGATCCATAATCCTCACCATAGTAC
Tesson_IgM_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
G12
001-110232556R
CACGCATGCACACACACACAAAC
Tesson_IgM_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
H11
007-110768688F
TGACCAGGAGTCTTGCTGAAGCT
Tesson_IgM_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
H12
007-110768688R
GGCTCTGGTCCCTTATAGATGATG
Tesson_IgM_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
Primer Plate #3
A1
002-111467723F
CCTGAGTCCAGGAAGAGTTGGT
Tesson_IgM_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
A2
002-111467723R
CCTCACGGACATGGTATTTGCATC
Tesson_IgM_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
B1
006-111938978F
AGGGGCACGTGGAGGATGAT
Tesson_IgM_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
B2
006-111938978R
TGCAGGGGACACTTTGTCCTAC
Tesson_IgM_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
C1
008-114686715F
CTGGTGTGTGTGAAGACAGCAAG
Tesson_IgM_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
C2
008-114686715R
GAGATAGCCTCAGGGAGCAC
Tesson_IgM_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
D1
010-000118878F
CCCAGGTATTCTGCCATAGTAGC
Tesson_IgM_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
D2
010-000118878R
CCACTGGTGTCCTCTGAATTTCC
Tesson_IgM_Ranking-Homology_5T	Primer pair generated using optimal parameters.