PROGNOS Summary for S2_S5_NN_Ranking-TALENv2.0

The hg19 genome was searched for the TALEN pair targeting:
5'- TCACCTTGCCCCACAGGGCAGT NN..NN ACAGGAGTCAGGTGCA -3'

Using these RVDs:
Left:  01HD02NI03HD04HD05NG06NG07NN08HD09HD10HD11HD12NI13HD14NI15NN16NN17NN18HD19NI20NN21NG
Right: 01NN02HD03NI04HD05HD06NG07NN08NI09HD10NG11HD12HD13NG14NN15NG

Up to 5 mismatches were allowed in each nuclease half-site.

Allowed spacing distances were: 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30

Homodimers and heterodimers of the nucleases were included in the output

7329 total sites were located
Mismatch Types
Genomic Regions
  0 and 2: 1
  2 and 2: 1
  3 and 3: 5
  2 and 5: 18
  3 and 4: 26
  3 and 5: 182
  4 and 4: 102
  4 and 5: 1568
  5 and 5: 5426
  Exons: 358
  Promoters: 77
  Introns: 3048
  Intergenic: 3846


The top 200 sites are ranked below according to parameters optimized for TALEN off-target prediction.
For the full output, click here.

This output will be deleted from the server one week after it is generated.  Please save your results.

Primer pairs were designed using the 400 bp of sequence on either side of the cleavage site to melt close to 60 °C

For easy visualization of cleavage on gels:
The smallest fragment will be longer than 130 bp, which is also the minimum separation distance between uncleaved and cleaved products.
The two cleavage products will be separated by a minimum size of 0 bp
To optimize assay conditions, the amplicons have a minimum length of 260 bp, a maximum length of 450 bp, and an optimal length of 270 bp.
Rank: 1	Homology Score: 52	TALEN Score: 83.91
	Genomic Region: Exon	Closest Gene: HBB	Caution! This site scores very closely to the intended nuclease target. The potential for cleavage at this site is of great concern.
Match Type: L16R
	           Left Nuclease
	         TCACCTTGCCCCACAGGGCAGT
	5'-CACGT TCACCTTGCCCCACAGGGCAGT        AACGGCAGACTTCTCC              TCAGGAGTCAGATGCA CCATG-3'
	3'-GTGCA AGTGGAACGGGGTGTCCCGTCA        TTGCCGTCTGAAGAGG              AGTCCTCAGTCTACGT GGTAC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 011-005248069
Forward Primer: 5'-GTGCCTATCAGAAACCCAAGAGTC-3' Tm: 59.038
Reverse Primer: 5'-CAATCTACTCCCAGGAGCAGG-3' Tm: 59.378
Expected PCR Product Length: 308
Expected CelI Cleavage Product Sizes: 154, 154
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr11:5248194-5248247

Rank: 2	Homology Score: 32	TALEN Score: 70.19
	Genomic Region: Exon	Closest Gene: HBD	Caution! This site scores very closely to the intended nuclease target. The potential for cleavage at this site is of great concern.
Match Type: L16R
	           Left Nuclease
	         TCACCTTGCCCCACAGGGCAGT
	5'-CACGT TCACTTTGCCCCACAGGGCATT        GACAGCAGTCTTCTCC              TCAGGAGTCAGATGCA CCATG-3'
	3'-GTGCA AGTGAAACGGGGTGTCCCGTAA        CTGTCGTCAGAAGAGG              AGTCCTCAGTCTACGT GGTAC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 011-005255477
Forward Primer: 5'-GTCAGTGCCTATCAGAAACCCAAG-3' Tm: 59.038
Reverse Primer: 5'-TCACTGGAGCAGGGAGGACA-3' Tm: 59.508
Expected PCR Product Length: 296
Expected CelI Cleavage Product Sizes: 158, 138
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr11:5255606-5255659

Rank: 3	Homology Score: 18	TALEN Score: 61.86
	Genomic Region: Intron	Closest Gene: FAM3D
Match Type: R17R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CCCTG TGCCCCTGACTCCTTA             CAGCTGTTCTAGAATGT              AAATGAGGCAGGTGCA TTTCG-3'
	3'-GGGAC ACGGGGACTGAGGAAT             GTCGACAAGATCTTACA              TTTACTCCGTCCACGT AAAGC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 003-058640715
Forward Primer: 5'-CATATGAGGAAACTGAGAGGCGG-3' Tm: 59.148
Reverse Primer: 5'-CCTCGAGGAGGGCTATTCTAGA-3' Tm: 59.258
Expected PCR Product Length: 283
Expected CelI Cleavage Product Sizes: 130, 153
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr3:58640819-58640867

Rank: 4	Homology Score: 17	TALEN Score: 61.29
	Genomic Region: Intergenic	Closest Gene: GPR6
Match Type: R30R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CCATC TCCACCTGGCTCCTGT       GTGGTCCATGTTGCAAGTTTTCAGGAAGGA       GCAGGAGTTAAGGGTA GAGTT-3'
	3'-GGTAG AGGTGGACCGAGGACA       CACCAGGTACAACGTTCAAAAGTCCTTCCT       CGTCCTCAATTCCCAT CTCAA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 006-110284189
Forward Primer: 5'-GCAATGTTGCTCCAAGAGGCC-3' Tm: 59.378
Reverse Primer: 5'-GAACTTATTCCTCCCACCTAGCTG-3' Tm: 59.038
Expected PCR Product Length: 297
Expected CelI Cleavage Product Sizes: 131, 166
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr6:110284287-110284348

Rank: 5	Homology Score: 17	TALEN Score: 61.17
	Genomic Region: Intron	Closest Gene: PARD3B
Match Type: R30R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GGAGT AGAAACTGATTCCTAT       GCAAGCAAAAATAACATAGGGGAACTGACC       ACAGGAGTTAGGTGGA GTTAG-3'
	3'-CCTCA TCTTTGACTAAGGATA       CGTTCGTTTTTATTGTATCCCCTTGACTGG       TGTCCTCAATCCACCT CAATC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 002-205984816
Forward Primer: 5'-GTGGGTACTGGCTGCTAAGTATG-3' Tm: 59.148
Reverse Primer: 5'-GTTCCTAGCAGCCACAGCAC-3' Tm: 59.508
Expected PCR Product Length: 428
Expected CelI Cleavage Product Sizes: 256, 172
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr2:205985039-205985100

Rank: 6	Homology Score: 18	TALEN Score: 60.84
	Genomic Region: Intron	Closest Gene: PHF21B
Match Type: R12R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CATTC TCCACCTGTCTCCTGC                CTCTCAACTGGG                TCTGGAGTCAGGTGGA AAAAT-3'
	3'-GTAAG AGGTGGACAGAGGACG                GAGAGTTGACCC                AGACCTCAGTCCACCT TTTTA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 022-045287459
Forward Primer: 5'-CAAAGGCTCTGCCTGCAGGA-3' Tm: 59.508
Reverse Primer: 5'-CAGGACTAAGGCCGCTCTTC-3' Tm: 59.508
Expected PCR Product Length: 269
Expected CelI Cleavage Product Sizes: 136, 133
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr22:45287571-45287614

Rank: 7	Homology Score: 17	TALEN Score: 60.33
	Genomic Region: Intron	Closest Gene: ATRN
Match Type: R12R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GCCAC TGCACCTGACCCCTAT                TATCTTCTTTTG                AAATGAGTCATCTGCT AATTC-3'
	3'-CGGTG ACGTGGACTGGGGATA                ATAGAAGAAAAC                TTTACTCAGTAGACGA TTAAG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 020-003459687
Forward Primer: 5'-GCCAGTGTGCCCAGCCAATT-3' Tm: 59.508
Reverse Primer: 5'-GTGTACGTAGCTGTCTTCCCTTC-3' Tm: 59.148
Expected PCR Product Length: 323
Expected CelI Cleavage Product Sizes: 164, 159
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr20:3459827-3459870

Rank: 8	Homology Score: 18	TALEN Score: 60.15
	Genomic Region: Intron	Closest Gene: PDE4D
Match Type: R29R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CACAC TGCACCTAACATCTGT       CCTTATAATAAACTAGGAGCATCTCTGTT        ACAGGAATCATGTTCA GTAGT-3'
	3'-GTGTG ACGTGGATTGTAGACA       GGAATATTATTTGATCCTCGTAGAGACAA        TGTCCTTAGTACAAGT CATCA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 005-059619945
Forward Primer: 5'-CCAAGACTTAGCTTAGCACCCAAC-3' Tm: 59.038
Reverse Primer: 5'-CATGCCTGGAGTTAGCCTCTCT-3' Tm: 59.258
Expected PCR Product Length: 384
Expected CelI Cleavage Product Sizes: 219, 165
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr5:59620132-59620192

Rank: 9	Homology Score: 13	TALEN Score: 58.72
	Genomic Region: Intron	Closest Gene: C14orf166B
Match Type: R24R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TAACT TGCCCCCGACTCATGT          TTTCTTAGATTATCTCTGGCAATC          ACAGGAGGCAGAGGTA AAGCT-3'
	3'-ATTGA ACGGGGGCTGAGTACA          AAAGAATCTAATAGAGACCGTTAG          TGTCCTCCGTCTCCAT TTCGA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 014-077327606
Forward Primer: 5'-GCCTGGTATAGAACAAGCACCTG-3' Tm: 59.148
Reverse Primer: 5'-CCTCCTGTGATTGCCAGAGC-3' Tm: 59.508
Expected PCR Product Length: 368
Expected CelI Cleavage Product Sizes: 179, 189
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr14:77327755-77327810

Rank: 10	Homology Score: 17	TALEN Score: 58.71
	Genomic Region: Intron	Closest Gene: CARD11
Match Type: R17R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GGGGG TGCACCTGCCTCCTGC             CTGCACCCTGGGAGCTT              GCAGGATGCAGAAGCA TGGGC-3'
	3'-CCCCC ACGTGGACGGAGGACG             GACGTGGGACCCTCGAA              CGTCCTACGTCTTCGT ACCCG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 007-002962408
Forward Primer: 5'-ACAGCGTTTGTCTCCTGGCC-3' Tm: 59.508
Reverse Primer: 5'-GCCCCTAAGCCTTGCATTCAG-3' Tm: 59.378
Expected PCR Product Length: 298
Expected CelI Cleavage Product Sizes: 167, 131
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr7:2962549-2962597

Rank: 11	Homology Score: 17	TALEN Score: 58.68
	Genomic Region: Exon	Closest Gene: LOC390660
Match Type: R27R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CTGCC CGCCGCTGCCACCTGT        GGCCCTGTGCCACCTGGCACTCAGGCA         ACTGGAGTAAGGTGCA TGAGG-3'
	3'-GACGG GCGGCGACGGTGGACA        CCGGGACACGGTGGACCGTGAGTCCGT         TGACCTCATTCCACGT ACTCC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 015-082621505
Forward Primer: 5'-TGGCTTTCACGGGTCTGTGC-3' Tm: 59.508
Reverse Primer: 5'-TAGACATGCAGCCTGGAGCC-3' Tm: 59.508
Expected PCR Product Length: 296
Expected CelI Cleavage Product Sizes: 157, 139
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr15:82621631-82621689

Rank: 12	Homology Score: 17	TALEN Score: 58.68
	Genomic Region: Exon	Closest Gene: LOC390660
Match Type: R27R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CTGCC CGCCGCTGCCACCTGT        GGCCCTGTGCCACCTGGCACTCAGGCA         ACTGGAGTAAGGTGCA TGAGG-3'
	3'-GACGG GCGGCGACGGTGGACA        CCGGGACACGGTGGACCGTGAGTCCGT         TGACCTCATTCCACGT ACTCC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 015-082918832
Forward Primer: 5'-TGGCTTTCACGGGTCTGTGC-3' Tm: 59.508
Reverse Primer: 5'-TAGACATGCAGCCTGGAGCC-3' Tm: 59.508
Expected PCR Product Length: 296
Expected CelI Cleavage Product Sizes: 157, 139
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr15:82918958-82919016

Rank: 13	Homology Score: 17	TALEN Score: 58.68
	Genomic Region: Intergenic	Closest Gene: LOC642423
Match Type: R27R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CCTCA TGCACCTTACTCCAGT        TGCCTGAGTGCCAGGTGGCACAGGGCC         ACAGGTGGCAGCGGCG GGCAG-3'
	3'-GGAGT ACGTGGAATGAGGTCA        ACGGACTCACGGTCCACCGTGTCCCGG         TGTCCACCGTCGCCGC CCGTC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 015-085803797
Forward Primer: 5'-TAGACATGCAGCCTGGAGCC-3' Tm: 59.508
Reverse Primer: 5'-TGGCTTTCACGGGTCTGTGC-3' Tm: 59.508
Expected PCR Product Length: 296
Expected CelI Cleavage Product Sizes: 142, 154
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr15:85803908-85803966

Rank: 14	Homology Score: 10	TALEN Score: 58.57
	Genomic Region: Intergenic	Closest Gene: FAM47A
Match Type: R10R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-AGTCT TCACCCTTACTCCTAT                 GCTTATTCTA                 ACAGGAGTTAGATGTA CTTCT-3'
	3'-TCAGA AGTGGGAATGAGGATA                 CGAATAAGAT                 TGTCCTCAATCTACAT GAAGA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 00X-034076224
Forward Primer: 5'-AGTATTGCCCTTTCTGCTAGGGAC-3' Tm: 59.038
Reverse Primer: 5'-CACTGCACACTTCTGTGGCTC-3' Tm: 59.378
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 134, 136
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chrX:34076335-34076376

Rank: 15	Homology Score: 18	TALEN Score: 58.44
	Genomic Region: Intergenic	Closest Gene: PKIB
Match Type: R29R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TTTAG CACACCTGACTGCTGT       GGAGTGTCCCAGCAGAAGGTTGTCCACGT        ACAGGAGCAAGATGCA GCCTA-3'
	3'-AAATC GTGTGGACTGACGACA       CCTCACAGGGTCGTCTTCCAACAGGTGCA        TGTCCTCGTTCTACGT CGGAT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 006-123066634
Forward Primer: 5'-GTAGTGGGATCGTGCCTGTG-3' Tm: 59.508
Reverse Primer: 5'-TCAGTACACTTGGACCCGGC-3' Tm: 59.508
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 135, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr6:123066737-123066797

Rank: 16	Homology Score: 13	TALEN Score: 58.4
	Genomic Region: Intergenic	Closest Gene: HYDIN2
Match Type: R26R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CCCTC TGCACACAACTCCTAT         GCACTCTGCACGCACCACCCTCCTGC         ACAGGTGTCCCGTGCA GGCTG-3'
	3'-GGGAG ACGTGTGTTGAGGATA         CGTGAGACGTGCGTGGTGGGAGGACG         TGTCCACAGGGCACGT CCGAC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: dom-000216374
Forward Primer: 5'-TCTCCTTTCTGTACCTCAGAGACC-3' Tm: 59.038
Reverse Primer: 5'-CCCACCCTGGTACAGCAAATG-3' Tm: 59.378
Expected PCR Product Length: 318
Expected CelI Cleavage Product Sizes: 131, 187
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr1_gl000192_random:216474-216531

Rank: 17	Homology Score: 13	TALEN Score: 58.34
	Genomic Region: Intergenic	Closest Gene: PLCL1
Match Type: R13R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GCAGA TACACCTGACTTCTAT               TTGAGGCCCAGAT                ACTGGAGACAGGAGCC TTATT-3'
	3'-CGTCT ATGTGGACTGAAGATA               AACTCCGGGTCTA                TGACCTCTGTCCTCGG AATAA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 002-199208411
Forward Primer: 5'-GCCTTACACATAACCTCACTGCC-3' Tm: 59.148
Reverse Primer: 5'-CTCCAACATAACTCAGCCTCGAAG-3' Tm: 59.038
Expected PCR Product Length: 393
Expected CelI Cleavage Product Sizes: 166, 227
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr2:199208553-199208597

Rank: 18	Homology Score: 13	TALEN Score: 58.12
	Genomic Region: Intron	Closest Gene: CACNA2D3
Match Type: R11R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CAAGG TGCCCCACACTCCTGT                GGTCTGGAAGG                 AATGGAGCCATGTGCA CACTT-3'
	3'-GTTCC ACGGGGTGTGAGGACA                CCAGACCTTCC                 TTACCTCGGTACACGT GTGAA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 003-054731501
Forward Primer: 5'-CCTACAGTTTCCAAGGACAAGCAG-3' Tm: 59.038
Reverse Primer: 5'-CCCAGCAGTAGCTTTAGCATCC-3' Tm: 59.258
Expected PCR Product Length: 320
Expected CelI Cleavage Product Sizes: 160, 160
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr3:54731638-54731680

Rank: 19	Homology Score: 13	TALEN Score: 57.73
	Genomic Region: Intron	Closest Gene: HYDIN
Match Type: R26R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CCCTC TGCACACAACTCCTAT         GCACTCTGCACGCACCACCCTCCTGC         ACAGGCGTCCCGTGCA GGCTG-3'
	3'-GGGAG ACGTGTGTTGAGGATA         CGTGAGACGTGCGTGGTGGGAGGACG         TGTCCGCAGGGCACGT CCGAC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 016-071012605
Forward Primer: 5'-CTCCTAGCTTCCTGATTCTTTGCC-3' Tm: 59.038
Reverse Primer: 5'-CCCACCCTGGTACAGCAAATG-3' Tm: 59.378
Expected PCR Product Length: 355
Expected CelI Cleavage Product Sizes: 168, 187
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr16:71012742-71012799

Rank: 20	Homology Score: 17	TALEN Score: 57.71
	Genomic Region: Intergenic	Closest Gene: TRHDE-AS1
Match Type: R28R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CCTCC TAGACCTGACTCCTGT        ATTTGTTTCCTCACACTTAAAGGGAACT        CAATGAGTCAGGTGAG TACTG-3'
	3'-GGAGG ATCTGGACTGAGGACA        TAAACAAAGGAGTGTGAATTTCCCTTGA        GTTACTCAGTCCACTC ATGAC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Warning! Primer pair was generated using suboptimal parameters. 
Primer Index: 012-072604585
Forward Primer: 5'-GTTGAGAGTTGACACCAACTTCTTTTCTTTTTCC-3' Tm: 59.668
Reverse Primer: 5'-CCACTCCTAGTGTGGAACCTC-3' Tm: 59.378
Expected PCR Product Length: 393
Expected CelI Cleavage Product Sizes: 263, 130
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr12:72604816-72604875

Rank: 21	Homology Score: 8	TALEN Score: 57.53
	Genomic Region: Intron	Closest Gene: HOMER1
Match Type: R15R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-AAGGA TACCCCTAAATCCTGT              CATTCTCCCACTTAA               CCTGGATTCAGGTGAA GACCT-3'
	3'-TTCCT ATGGGGATTTAGGACA              GTAAGAGGGTGAATT               GGACCTAAGTCCACTT CTGGA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 005-078702362
Forward Primer: 5'-GAAAGGATCCTGGATGAGGGG-3' Tm: 59.378
Reverse Primer: 5'-GTAGGAGTTTCCTGCCTCTGTC-3' Tm: 59.258
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 132, 138
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr5:78702469-78702515

Rank: 22	Homology Score: 17	TALEN Score: 57.47
	Genomic Region: Intergenic	Closest Gene: SLC2A10
Match Type: R24R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CCCCT TCCTCCAGCTTCCTGT          AGGTTCAACTAGGGGGAAGCCCCA          GCAGGAGACAGGTGCA CAGTG-3'
	3'-GGGGA AGGAGGTCGAAGGACA          TCCAAGTTGATCCCCCTTCGGGGT          CGTCCTCTGTCCACGT GTCAC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 020-045428486
Forward Primer: 5'-GTCTCACTGAGACCTAGGCG-3' Tm: 59.508
Reverse Primer: 5'-TGGGCCTGGGGAACCAACAA-3' Tm: 59.508
Expected PCR Product Length: 268
Expected CelI Cleavage Product Sizes: 133, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr20:45428589-45428644

Rank: 23	Homology Score: 17	TALEN Score: 57.42
	Genomic Region: Intron	Closest Gene: SDK1
Match Type: R13R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TGTTT TGAACCTGACTCCTTT               CACATGGCATAGC                ACATGTGACACATGCA TATCA-3'
	3'-ACAAA ACTTGGACTGAGGAAA               GTGTACCGTATCG                TGTACACTGTGTACGT ATAGT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 007-003795640
Forward Primer: 5'-CCTGACTCCTTTCACATGGCATAG-3' Tm: 59.038
Reverse Primer: 5'-TGTGTCACACGTGCTATGCCATG-3' Tm: 59.148
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 135, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr7:3795751-3795795

Rank: 24	Homology Score: 17	TALEN Score: 57.42
	Genomic Region: Intron	Closest Gene: SDK1
Match Type: R13R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TGTTT TGAACCTGACTCCTTT               CACATGGCATAGC                ACATGTGACACATGCA TATCA-3'
	3'-ACAAA ACTTGGACTGAGGAAA               GTGTACCGTATCG                TGTACACTGTGTACGT ATAGT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 007-003795876
Forward Primer: 5'-GAGTCCTTTCACATGGCATAGCAC-3' Tm: 59.038
Reverse Primer: 5'-ACATGTCTCACACGCACAATGCC-3' Tm: 59.148
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 135, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr7:3795987-3796031

Rank: 25	Homology Score: 10	TALEN Score: 57.42
	Genomic Region: Intron	Closest Gene: POTEF
Match Type: R29R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-AGGAT TGCAGCCAGCTCCTGC       CCCGGTGCAGTGTATACAGTGTATACTGC        ACAGGTGTTGGGTGCA CCAAA-3'
	3'-TCCTA ACGTCGGTCGAGGACG       GGGCCACGTCACATATGTCACATATGACG        TGTCCACAACCCACGT GGTTT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Warning! Primer pair was generated using suboptimal parameters. 
Primer Index: 002-130879063
Forward Primer: 5'-AAGACGCCCACCGAGCCCA-3' Tm: 62.008
Reverse Primer: 5'-GCTAATGGGTGCAAAGTTTCTTTTAAGGAGCATA-3' Tm: 59.668
Expected PCR Product Length: 450
Expected CelI Cleavage Product Sizes: 144, 306
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr2:130879175-130879235

Rank: 26	Homology Score: 10	TALEN Score: 57.42
	Genomic Region: Intergenic	Closest Gene: POTEE
Match Type: R29R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TTTGG TGCACCCAACACCTGT       GCAGTATACACTGTATACACTGCACCGGG        GCAGGAGCTGGCTGCA ATCCT-3'
	3'-AAACC ACGTGGGTTGTGGACA       CGTCATATGTGACATATGTGACGTGGCCC        CGTCCTCGACCGACGT TAGGA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Warning! Primer pair was generated using suboptimal parameters. 
Primer Index: 002-131974522
Forward Primer: 5'-GCTAATGGGTGCAAAGTTTCTTTTAAGGAGCATA-3' Tm: 59.668
Reverse Primer: 5'-AAGACGCCCACCGAGCCCA-3' Tm: 62.008
Expected PCR Product Length: 450
Expected CelI Cleavage Product Sizes: 309, 141
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr2:131974799-131974859

Rank: 27	Homology Score: 13	TALEN Score: 57.41
	Genomic Region: Intergenic	Closest Gene: SGCZ
Match Type: R10R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-ATGGA TGCTCCTGCCACCTGT                 GGAGCCAGCC                 TCAGGAGGCAGGTGAG GGAGG-3'
	3'-TACCT ACGAGGACGGTGGACA                 CCTCGGTCGG                 AGTCCTCCGTCCACTC CCTCC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 008-015147764
Forward Primer: 5'-CCCCACTTCATCTGCTAAACTTCC-3' Tm: 59.038
Reverse Primer: 5'-GAGGGGGAAAATGTGGGAAGTTCT-3' Tm: 59.038
Expected PCR Product Length: 313
Expected CelI Cleavage Product Sizes: 168, 145
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr8:15147909-15147950

Rank: 28	Homology Score: 10	TALEN Score: 57.38
	Genomic Region: Intron	Closest Gene: C14orf166B
Match Type: R24R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TAACT TGCCCCCAACTCATCT          TTTCTTAGATTAGCTCTGGCAATC          ACAGGAGGCAGATGTA ACAGT-3'
	3'-ATTGA ACGGGGGTTGAGTAGA          AAAGAATCTAATCGAGACCGTTAG          TGTCCTCCGTCTACAT TGTCA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 014-077327823
Forward Primer: 5'-CCAACAGCTGGTAAGGATGTCAAG-3' Tm: 59.038
Reverse Primer: 5'-CTGTGGAAACACCAAAGCAGGG-3' Tm: 59.258
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 133, 137
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr14:77327926-77327981

Rank: 29	Homology Score: 10	TALEN Score: 57.37
	Genomic Region: Intergenic	Closest Gene: NDST3
Match Type: R26R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TTACA TGCATCTATCTCATTT         AATGCTTTTAACAATGCTACAACTAA         AAAGTAGTCAGGTGTA GACTG-3'
	3'-AATGT ACGTAGATAGAGTAAA         TTACGAAAATTGTTACGATGTTGATT         TTTCATCAGTCCACAT CTGAC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Warning! Primer pair was generated using suboptimal parameters. 
Primer Index: 004-118702135
Forward Primer: 5'-GCAGGGACACTGGTGTTATGTCT-3' Tm: 59.148
Reverse Primer: 5'-GATAGTAACTTATTTAGAATTGAGGTTGTGTGATGGAG-3' Tm: 59.358
Expected PCR Product Length: 429
Expected CelI Cleavage Product Sizes: 293, 136
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr4:118702397-118702454

Rank: 30	Homology Score: 17	TALEN Score: 57.25
	Genomic Region: Intergenic	Closest Gene: SH3BP4
Match Type: R23R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GCAAT TGCACCTCACTTCTGT          GGAGTGCTTCCTGTGTATCAGGC           ACAGAAGCCCGATGTA TTCTC-3'
	3'-CGTTA ACGTGGAGTGAAGACA          CCTCACGAAGGACACATAGTCCG           TGTCTTCGGGCTACAT AAGAG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 002-235805535
Forward Primer: 5'-ACCTCCACCAGACCACGAATG-3' Tm: 59.378
Reverse Primer: 5'-GACCCTTAGCAGGTCACACTC-3' Tm: 59.378
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 135, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr2:235805641-235805695

Rank: 31	Homology Score: 17	TALEN Score: 57.21
	Genomic Region: Exon	Closest Gene: XPNPEP1
Match Type: R20R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-AGGGA TGCACCTGGATCCTGT            ATTCGGCTTCCAGACCCAAG            TCAAGAAGCAGGTGCT CCTTC-3'
	3'-TCCCT ACGTGGACCTAGGACA            TAAGCCGAAGGTCTGGGTTC            AGTTCTTCGTCCACGA GGAAG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 010-111642202
Forward Primer: 5'-CTCGCTCACAGCATAGCTGG-3' Tm: 59.508
Reverse Primer: 5'-TATGTGTGTCTGTGTTGCCACCTC-3' Tm: 59.038
Expected PCR Product Length: 273
Expected CelI Cleavage Product Sizes: 131, 142
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr10:111642305-111642356

Rank: 32	Homology Score: 13	TALEN Score: 57.06
	Genomic Region: Intergenic	Closest Gene: GPC1
Match Type: R29R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GAGCC TTCACCTGATTCCAGC       CGCAAAGCCCTTTCCAGTACGGCCCATTC        ACAGGTTCCAGGTGCA AAGCC-3'
	3'-CTCGG AAGTGGACTAAGGTCG       GCGTTTCGGGAAAGGTCATGCCGGGTAAG        TGTCCAAGGTCCACGT TTCGG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 002-241334168
Forward Primer: 5'-CATGCACCCCTGGCTTGTGT-3' Tm: 59.508
Reverse Primer: 5'-AGCTTTGCACCTGGAACCTGTGA-3' Tm: 59.148
Expected PCR Product Length: 297
Expected CelI Cleavage Product Sizes: 138, 159
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr2:241334274-241334334

Rank: 33	Homology Score: 18	TALEN Score: 56.93
	Genomic Region: Intergenic	Closest Gene: FLJ11235
Match Type: R29R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TAGGC TGCATCTTGCTCCTGT       ACATGGATGACCTTCTGCTGGGACACTCC        ACGGCAGTCAGGTGCG CAAAA-3'
	3'-ATCCG ACGTAGAACGAGGACA       TGTACCTACTGGAAGACGACCCTGTGAGG        TGCCGTCAGTCCACGC GTTTT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 005-111863094
Forward Primer: 5'-AGTACACTTGGACCCAGCTTACC-3' Tm: 59.148
Reverse Primer: 5'-TGAATCCCAGGTAGCGTACCTGC-3' Tm: 61.088
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 136, 134
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr5:111863198-111863258

Rank: 34	Homology Score: 13	TALEN Score: 56.58
	Genomic Region: Intergenic	Closest Gene: ZNF812
Match Type: R29R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TTTTG TGCACCCAACTGCTGT       GGAGTGTCCCAGCAGAACGTCGTCCATGT        ACAGGAGCAAGACGCA GCCTA-3'
	3'-AAAAC ACGTGGGTTGACGACA       CCTCACAGGGTCGTCTTGCAGCAGGTACA        TGTCCTCGTTCTGCGT CGGAT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 019-009837811
Forward Primer: 5'-AATCCCAGGTAGCGTACGTGC-3' Tm: 59.378
Reverse Primer: 5'-TCAGTACACATGGACCTGGCTTC-3' Tm: 59.148
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 135, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr19:9837914-9837974

Rank: 35	Homology Score: 10	TALEN Score: 56.55
	Genomic Region: Intron	Closest Gene: LOC100507605
Match Type: R27R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TCTGG CGCAACTCACCCCTGG        CTTGCACCAAACATTCTTAAAATGTTT         ATTGGGGTCAGGTGCA ATGGC-3'
	3'-AGACC GCGTTGAGTGGGGACC        GAACGTGGTTTGTAAGAATTTTACAAA         TAACCCCAGTCCACGT TACCG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 010-029082810
Forward Primer: 5'-CCTCTGAGCCCCATTCTCCT-3' Tm: 59.508
Reverse Primer: 5'-TAGAGATGGGGTCTCACCTTGTTG-3' Tm: 59.038
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 135, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr10:29082914-29082972

Rank: 36	Homology Score: 5	TALEN Score: 56.53
	Genomic Region: Intergenic	Closest Gene: LYSMD4
Match Type: R30L
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GAGCG TGCCCCTGGCTCCCTT       CTGCCTCCTGACCAACACTGGTGCATCCCT ATGGCCCTGTGGGGCCTGGTGT GCTCC-3'
	3'-CTCGC ACGGGGACCGAGGGAA       GACGGAGGACTGGTTGTGACCACGTAGGGA TACCGGGACACCCCGGACCACA CGAGG-5'
	                                                               TGACGGGACACCCCGTTCCACT
	                                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 015-100263695
Forward Primer: 5'-GACTGAGGAGAGACTGCTGTTC-3' Tm: 59.258
Reverse Primer: 5'-GTGGAGACCTTAAGAGATGTCTCG-3' Tm: 59.038
Expected PCR Product Length: 304
Expected CelI Cleavage Product Sizes: 131, 173
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr15:100263790-100263857

Rank: 37	Homology Score: 10	TALEN Score: 56.47
	Genomic Region: Intron	Closest Gene: SLC2A13
Match Type: R19R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TAAGA TTCAACTGACTCCTGA            GTTAGATGCAAAAAATTTT             ATAGTAGTGAGGTCTA CTGTT-3'
	3'-ATTCT AAGTTGACTGAGGACT            CAATCTACGTTTTTTAAAA             TATCATCACTCCAGAT GACAA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 012-040234817
Forward Primer: 5'-CCTTACAATTCACGGCTCTCAGAC-3' Tm: 59.038
Reverse Primer: 5'-CCACTGGAGTTGACAGATTTGGC-3' Tm: 59.148
Expected PCR Product Length: 436
Expected CelI Cleavage Product Sizes: 130, 306
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr12:40234920-40234970

Rank: 38	Homology Score: 10	TALEN Score: 56.42
	Genomic Region: Intron	Closest Gene: COL23A1
Match Type: R29R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-ATCCC TGCACCTCACACCTAT       CCTGAAGCAAACTAATCAGTAGTTAAATA        AGAGGAGGAGGGTACA TTACG-3'
	3'-TAGGG ACGTGGAGTGTGGATA       GGACTTCGTTTGATTAGTCATCAATTTAT        TCTCCTCCTCCCATGT AATGC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 005-177943719
Forward Primer: 5'-AAGCTTTCTGCCTCCTCAGGCC-3' Tm: 61.288
Reverse Primer: 5'-CACCTCAGTGACAGGCGCTT-3' Tm: 59.508
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 137, 133
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr5:177943824-177943884

Rank: 39	Homology Score: 17	TALEN Score: 56.4
	Genomic Region: Intron	Closest Gene: PAK4
Match Type: R27R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TTGTC CTCCCCTTGCTCCTGT        CCATTCCGACTCTCACCCATTCTGTGG         ACAGGAGTCAAGTGCT TATTC-3'
	3'-AACAG GAGGGGAACGAGGACA        GGTAAGGCTGAGAGTGGGTAAGACACC         TGTCCTCAGTTCACGA ATAAG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 019-039625781
Forward Primer: 5'-CCTCACCAACATTTGGTACCGTC-3' Tm: 59.148
Reverse Primer: 5'-CGGAGGTTGCAGTGAGCTGA-3' Tm: 59.508
Expected PCR Product Length: 282
Expected CelI Cleavage Product Sizes: 151, 131
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr19:39625901-39625959

Rank: 40	Homology Score: 10	TALEN Score: 56.4
	Genomic Region: Intron	Closest Gene: WNT10A
Match Type: R26R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GTGTC TGCCCCAGACAGCTGG         GGCCAGAACCGAAACTGGGGCCCTGA         ACAGGAGCTGGGTGCA GACCC-3'
	3'-CACAG ACGGGGTCTGTCGACC         CCGGTCTTGGCTTTGACCCCGGGACT         TGTCCTCGACCCACGT CTGGG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 002-219751327
Forward Primer: 5'-TGTGTCTTAACGAGCCGCCC-3' Tm: 59.508
Reverse Primer: 5'-CTGACAGCTGTCATAGCCACG-3' Tm: 59.378
Expected PCR Product Length: 284
Expected CelI Cleavage Product Sizes: 154, 130
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr2:219751450-219751507

Rank: 41	Homology Score: 10	TALEN Score: 56.32
	Genomic Region: Intergenic	Closest Gene: RPS29
Match Type: R29R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TGTTT TACACATGACTACTGT       TTTATTCCCTCTGTAAGTGGCAGTAATGC        TCAGGAGACAGGTTTC AATTA-3'
	3'-ACAAA ATGTGTACTGATGACA       AAATAAGGGAGACATTCACCGTCATTACG        AGTCCTCTGTCCAAAG TTAAT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 014-049949953
Forward Primer: 5'-GTTGTTCTTTCACCCACTGGAGC-3' Tm: 59.148
Reverse Primer: 5'-GACACATCAGAAAGACCGCTGAC-3' Tm: 59.148
Expected PCR Product Length: 377
Expected CelI Cleavage Product Sizes: 240, 137
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr14:49950161-49950221

Rank: 42	Homology Score: 13	TALEN Score: 56.31
	Genomic Region: Intergenic	Closest Gene: FRG1
Match Type: R14R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GTTTC TACCCCTGACCCCTGC               AGTCTACTCTGAGC               ACAGCAGTCAGAAGCA TTCTT-3'
	3'-CAAAG ATGGGGACTGGGGACG               TCAGATGAGACTCG               TGTCGTCAGTCTTCGT AAGAA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 004-190659833
Forward Primer: 5'-CTGCCTCCAATCCCTCAGAAAATG-3' Tm: 59.038
Reverse Primer: 5'-CTGGTATTGGGTCCAGACCAC-3' Tm: 59.378
Expected PCR Product Length: 308
Expected CelI Cleavage Product Sizes: 178, 130
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr4:190659986-190660031

Rank: 43	Homology Score: 13	TALEN Score: 56.28
	Genomic Region: Intergenic	Closest Gene: GAS2L3
Match Type: R29R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TTTTG CGCACCCGACTGCCGT       GGAGTGTTCCAGCAGAAGGTCGTCCACGT        ACAGGAGCTAGATGCA TCCTG-3'
	3'-AAAAC GCGTGGGCTGACGGCA       CCTCACAAGGTCGTCTTCCAGCAGGTGCA        TGTCCTCGATCTACGT AGGAC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 012-101038644
Forward Primer: 5'-CTGTGCTCCCCTTTCTGAATAGTG-3' Tm: 59.038
Reverse Primer: 5'-TACACTTGGACCCGACTTCCC-3' Tm: 59.378
Expected PCR Product Length: 272
Expected CelI Cleavage Product Sizes: 141, 131
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr12:101038753-101038813

Rank: 44	Homology Score: 13	TALEN Score: 56.23
	Genomic Region: Intergenic	Closest Gene: FAM41AY1
Match Type: R29R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TAGGC TGCATCTTACTCCAGT       ACATAGACAACCTTCTGCTAGGATACTCC        ACAGCAGTCGGGTGTG CAAGA-3'
	3'-ATCCG ACGTAGAATGAGGTCA       TGTATCTGTTGGAAGACGATCCTATGAGG        TGTCGTCAGCCCACAC GTTCT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 00Y-018946879
Forward Primer: 5'-ATACACTTGGACTCGGCTTCCC-3' Tm: 59.258
Reverse Primer: 5'-GTGAATCTCAGGTAGTGTACCTGC-3' Tm: 59.038
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 135, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chrY:18946982-18947042

Rank: 45	Homology Score: 5	TALEN Score: 56.22
	Genomic Region: Intron	Closest Gene: EXOC3L2
Match Type: R16R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-ACCTT TGCACCGAACCCCTAT              AGGGCGGGCACGAGGA              CCAGGAGATGGGTGTA TGGGG-3'
	3'-TGGAA ACGTGGCTTGGGGATA              TCCCGCCCGTGCTCCT              GGTCCTCTACCCACAT ACCCC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 019-045727578
Forward Primer: 5'-GAGAAGGGGAGTGTTCCACTG-3' Tm: 59.378
Reverse Primer: 5'-GGATAACAGAGGGCCATCAGC-3' Tm: 59.378
Expected PCR Product Length: 278
Expected CelI Cleavage Product Sizes: 148, 130
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr19:45727700-45727747

Rank: 46	Homology Score: 17	TALEN Score: 56.14
	Genomic Region: Intron	Closest Gene: CABIN1
Match Type: R10R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GGGGT TGCATCTGAATCCTGT                 GGGTGTGGCA                 GCAGGGGACAGCTGGA AAAGG-3'
	3'-CCCCA ACGTAGACTTAGGACA                 CCCACACCGT                 CGTCCCCTGTCGACCT TTTCC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 022-024555541
Forward Primer: 5'-CACAGCCATTCTGTCTACTCCC-3' Tm: 59.258
Reverse Primer: 5'-TGGCAGGTGGCCAGTGTCTT-3' Tm: 59.508
Expected PCR Product Length: 273
Expected CelI Cleavage Product Sizes: 140, 133
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr22:24555658-24555699

Rank: 47	Homology Score: 17	TALEN Score: 56.13
	Genomic Region: Intergenic	Closest Gene: GSTM3
Match Type: R15R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CCTAC TGCTGCTGTCTCCTTC              TAATTCTGTCGGACA               ACAGGAGTCAGGTTCT GAGTC-3'
	3'-GGATG ACGACGACAGAGGAAG              ATTAAGACAGCCTGT               TGTCCTCAGTCCAAGA CTCAG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 001-110268884
Forward Primer: 5'-CAGGCAGGCAATCTTGATGTGG-3' Tm: 59.258
Reverse Primer: 5'-CTCCTTTTTCCTGCGTAGCAAGTG-3' Tm: 59.038
Expected PCR Product Length: 283
Expected CelI Cleavage Product Sizes: 152, 131
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr1:110269011-110269057

Rank: 48	Homology Score: 10	TALEN Score: 56.11
	Genomic Region: Intergenic	Closest Gene: DKFZp686K1684
Match Type: R11R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GAACT TTCACCTGACTCCTTA                TGCTTAGCTCA                 AACGGGGGCAGGTTCA AGCAG-3'
	3'-CTTGA AAGTGGACTGAGGAAT                ACGAATCGAGT                 TTGCCCCCGTCCAAGT TCGTC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 011-031965225
Forward Primer: 5'-CCAGCCTGAGACAACCATCTTTAC-3' Tm: 59.038
Reverse Primer: 5'-GAAGGGCCATAACTTGGGCATTAG-3' Tm: 59.038
Expected PCR Product Length: 355
Expected CelI Cleavage Product Sizes: 192, 163
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr11:31965394-31965436

Rank: 49	Homology Score: 10	TALEN Score: 56.11
	Genomic Region: Intergenic	Closest Gene: MIR4686
Match Type: R25R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TCGGC TCCACCTGACTCCTAC         ACTGGCTCTGCAACCATTCCCAGCC          ACAGAGGTTGGCTGCA AGTAC-3'
	3'-AGCCG AGGTGGACTGAGGATG         TGACCGAGACGTTGGTAAGGGTCGG          TGTCTCCAACCGACGT TCATG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 011-002239013
Forward Primer: 5'-AGCTGTCCAGCCTGCAGCAT-3' Tm: 59.508
Reverse Primer: 5'-TCATATTCCTGGGTGGTCACTCC-3' Tm: 59.148
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 138, 132
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr11:2239121-2239177

Rank: 50	Homology Score: 10	TALEN Score: 56.1
	Genomic Region: Intron	Closest Gene: USP43
Match Type: R21R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TCATG TGAAACTGACTCCTGA           GAAAATGTGGGCAGGAATGAT            TTAGCAGGCAGGTGGA AGGTG-3'
	3'-AGTAC ACTTTGACTGAGGACT           CTTTTACACCCGTCCTTACTA            AATCGTCCGTCCACCT TCCAC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 017-009569404
Forward Primer: 5'-GGGAATGAAAAGGACAGCAGGAC-3' Tm: 59.148
Reverse Primer: 5'-CGGCCCTGCGCGTTATTTCT-3' Tm: 59.508
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 135, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr17:9569507-9569559

Rank: 51	Homology Score: 13	TALEN Score: 56.09
	Genomic Region: Intron	Closest Gene: GRIA2
Match Type: R19R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-ATCCA TACACCTGTCTGCTGT            AACCTTTTCCAGATTGGCT             ACTGGAGTGAAGTGGA CAAAA-3'
	3'-TAGGT ATGTGGACAGACGACA            TTGGAAAAGGTCTAACCGA             TGACCTCACTTCACCT GTTTT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
No Primers
Primer Index: No Primers
Forward Primer: 5'-No Primers-3' Tm: No Primers
Reverse Primer: 5'-No Primers-3' Tm: No Primers
Expected PCR Product Length: No Primers
Expected CelI Cleavage Product Sizes: No Primers, No Primers
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr4:158255134-158255184

Rank: 52	Homology Score: 10	TALEN Score: 56.07
	Genomic Region: Intron	Closest Gene: USP43
Match Type: R28R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TCATG TGAAACTGACTCCTGA        GAAAATGTGGGCAGGAATGATTTAGCAG        GCAGGTGGAAGGTGGA GAGAT-3'
	3'-AGTAC ACTTTGACTGAGGACT        CTTTTACACCCGTCCTTACTAAATCGTC        CGTCCACCTTCCACCT CTCTA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 017-009569404
Forward Primer: 5'-GGGAATGAAAAGGACAGCAGGAC-3' Tm: 59.148
Reverse Primer: 5'-CGGCCCTGCGCGTTATTTCT-3' Tm: 59.508
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 135, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr17:9569507-9569566

Rank: 53	Homology Score: 10	TALEN Score: 56.06
	Genomic Region: Intron	Closest Gene: COL25A1
Match Type: R11R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TGCAA TGCAGCGGGCTCCCCT                TACACACCTTT                 ACAGGAGTTAGTTGAA TATCA-3'
	3'-ACGTT ACGTCGCCCGAGGGGA                ATGTGTGGAAA                 TGTCCTCAATCAACTT ATAGT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 004-110007835
Forward Primer: 5'-GACTGCTGGGAATTGCTCTTTGAC-3' Tm: 59.038
Reverse Primer: 5'-TCCCTCAACTTCCACTGGGC-3' Tm: 59.508
Expected PCR Product Length: 290
Expected CelI Cleavage Product Sizes: 157, 133
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr4:110007969-110008011

Rank: 54	Homology Score: 10	TALEN Score: 56
	Genomic Region: Intergenic	Closest Gene: FOXP1
Match Type: R22R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GAGCT ATCACCTGACCCCTGT           TAGGATGGCTATTATAAAAAAG           TCAGAAGATAGGTGTA GGAGA-3'
	3'-CTCGA TAGTGGACTGGGGACA           ATCCTACCGATAATATTTTTTC           AGTCTTCTATCCACAT CCTCT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 003-070683485
Forward Primer: 5'-GGGCAAAGGATCTGAATAGACAATTTCCCA-3' Tm: 60.058
Reverse Primer: 5'-CACTGATGGACACTTCTGTTGCC-3' Tm: 59.148
Expected PCR Product Length: 384
Expected CelI Cleavage Product Sizes: 149, 235
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr3:70683605-70683658

Rank: 55	Homology Score: 13	TALEN Score: 55.98
	Genomic Region: Intron	Closest Gene: ERGIC1
Match Type: R10R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-AGGTC TGCTCCTGCCTCCAGT                 AGAAGCAGCC                 ACAGGAGTCCTGGCCA CAGCC-3'
	3'-TCCAG ACGAGGACGGAGGTCA                 TCTTCGTCGG                 TGTCCTCAGGACCGGT GTCGG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 005-172280139
Forward Primer: 5'-TGGTTTACCACGTGCTGTTGTTGC-3' Tm: 59.038
Reverse Primer: 5'-AAGATGCCTCTGGGTGGCCT-3' Tm: 59.508
Expected PCR Product Length: 272
Expected CelI Cleavage Product Sizes: 131, 141
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr5:172280247-172280288

Rank: 56	Homology Score: 13	TALEN Score: 55.92
	Genomic Region: Intron	Closest Gene: SORBS2
Match Type: R12R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-AATGT TTTTCCTGACTTCTGT                GATGTATAGGGC                ACTGTAGTCAGGTGAA ATAAC-3'
	3'-TTACA AAAAGGACTGAAGACA                CTACATATCCCG                TGACATCAGTCCACTT TATTG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 004-186747672
Forward Primer: 5'-CTGTGAGCTACTGAGGATATGCG-3' Tm: 59.148
Reverse Primer: 5'-GGGCAGATGCTTAAATCACTCCCA-3' Tm: 59.038
Expected PCR Product Length: 374
Expected CelI Cleavage Product Sizes: 218, 156
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr4:186747866-186747909

Rank: 57	Homology Score: 10	TALEN Score: 55.83
	Genomic Region: Intergenic	Closest Gene: DHRS2
Match Type: R10R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TCTCC TTCACCTGACTCTAGT                 TGTTGCTGGA                 ACAGGACTTTGTTGTA TATTC-3'
	3'-AGAGG AAGTGGACTGAGATCA                 ACAACGACCT                 TGTCCTGAAACAACAT ATAAG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 014-024242484
Forward Primer: 5'-CAAGTCTTCCTCAGCCATCAGACA-3' Tm: 59.038
Reverse Primer: 5'-CCAGACACATCAACAGAGTGGAAG-3' Tm: 59.038
Expected PCR Product Length: 298
Expected CelI Cleavage Product Sizes: 138, 160
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr14:24242599-24242640

Rank: 58	Homology Score: 13	TALEN Score: 55.82
	Genomic Region: Intron	Closest Gene: PBRM1
Match Type: R22R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TCTCC TGCAACTGAAGCCTGT           ATTTAATTTAAGCAGGAATGGC           ACTGGAATCAGGCTCA GCAGT-3'
	3'-AGAGG ACGTTGACTTCGGACA           TAAATTAAATTCGTCCTTACCG           TGACCTTAGTCCGAGT CGTCA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 003-052584959
Forward Primer: 5'-GGCTAGGAATCTTAAGCACCCC-3' Tm: 59.258
Reverse Primer: 5'-GAGGGGATAGCAAGGCTGTG-3' Tm: 59.508
Expected PCR Product Length: 378
Expected CelI Cleavage Product Sizes: 246, 132
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr3:52585176-52585229

Rank: 59	Homology Score: 10	TALEN Score: 55.79
	Genomic Region: Intron	Closest Gene: ZZEF1
Match Type: R29R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-ACTAC TGCCACAGACTCCTAA       CTGCTTTTCTTATTTCCACCTCTTTTCAC        ACAGCAGCCAGGGGCA CCTCT-3'
	3'-TGATG ACGGTGTCTGAGGATT       GACGAAAAGAATAAAGGTGGAGAAAAGTG        TGTCGTCGGTCCCCGT GGAGA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 017-003965044
Forward Primer: 5'-CGTAAGTCCTGACAACTCTACCTC-3' Tm: 59.038
Reverse Primer: 5'-GAAAGGCAGGACCCTTATCACTGT-3' Tm: 59.038
Expected PCR Product Length: 290
Expected CelI Cleavage Product Sizes: 143, 147
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr17:3965155-3965215

Rank: 60	Homology Score: 13	TALEN Score: 55.79
	Genomic Region: Intergenic	Closest Gene: RNU6-78
Match Type: R22R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TGAGT ATCACCTCACACCTGT           TAGGATGGCTATTATAAAAAAG           GCAGCAGCCAGGTGCA GTGGC-3'
	3'-ACTCA TAGTGGAGTGTGGACA           ATCCTACCGATAATATTTTTTC           CGTCGTCGGTCCACGT CACCG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 012-032161181
Forward Primer: 5'-GCTCAATGGCAGAAAACCAAATAACCCAAC-3' Tm: 60.058
Reverse Primer: 5'-GAGACGGGTTTTTGCCATGTTGC-3' Tm: 59.148
Expected PCR Product Length: 315
Expected CelI Cleavage Product Sizes: 185, 130
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr12:32161337-32161390

Rank: 61	Homology Score: 8	TALEN Score: 55.77
	Genomic Region: Intergenic	Closest Gene: RGS17
Match Type: R11R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CCTTG TGCAGCTGGCTCCCAT                GATGGCAGCCA                 AATGGAGGCTGGTGCA CTGTG-3'
	3'-GGAAC ACGTCGACCGAGGGTA                CTACCGTCGGT                 TTACCTCCGACCACGT GACAC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 006-153846994
Forward Primer: 5'-GCAAGCTGTCTCATGCTCACTG-3' Tm: 59.258
Reverse Primer: 5'-CAGGAAGGAACTTGTCAGATTCCC-3' Tm: 59.038
Expected PCR Product Length: 366
Expected CelI Cleavage Product Sizes: 229, 137
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr6:153847200-153847242

Rank: 62	Homology Score: 10	TALEN Score: 55.63
	Genomic Region: Exon	Closest Gene: DCHS1
Match Type: R23R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GCTCC TGCACCTGGCAGCAGC          AGCCCTGCCTTTCGGCCCTTATA           CCAGGTGTCAGGGGCA GGTGG-3'
	3'-CGAGG ACGTGGACCGTCGTCG          TCGGGACGGAAAGCCGGGAATAT           GGTCCACAGTCCCCGT CCACC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 011-006643499
Forward Primer: 5'-TGGGAAGCCATAGTCCCCAG-3' Tm: 59.508
Reverse Primer: 5'-ATCAATGAGTTCCCCCGTGTGG-3' Tm: 59.258
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 131, 139
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr11:6643601-6643655

Rank: 63	Homology Score: 10	TALEN Score: 55.61
	Genomic Region: Intron	Closest Gene: C1orf198
Match Type: R20R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GGGCT TGCCCTTGACACCTGT            CTCAGGCCCAGTTTGGCTAC            ATGTGAGACTGGTGCA GAGTG-3'
	3'-CCCGA ACGGGAACTGTGGACA            GAGTCCGGGTCAAACCGATG            TACACTCTGACCACGT CTCAC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 001-230976433
Forward Primer: 5'-GCGCCAAGGTCTTCTTGAGC-3' Tm: 59.508
Reverse Primer: 5'-GGAGAGTAGGAGACAGCACATG-3' Tm: 59.258
Expected PCR Product Length: 312
Expected CelI Cleavage Product Sizes: 136, 176
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr1:230976541-230976592

Rank: 64	Homology Score: 10	TALEN Score: 55.56
	Genomic Region: Intergenic	Closest Gene: MALAT1
Match Type: R14R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GAGCT TGCATCATCCTCCTGA               CCAGGGTGGAATAA               CCAGGAGGCAGGCGCA CGTGA-3'
	3'-CTCGA ACGTAGTAGGAGGACT               GGTCCCACCTTATT               GGTCCTCCGTCCGCGT GCACT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 011-065255104
Forward Primer: 5'-TGGGGGAGAAAGGGAAAGGC-3' Tm: 59.508
Reverse Primer: 5'-AGGCGGAGGCAGAGTAGGTA-3' Tm: 59.508
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 135, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr11:65255214-65255259

Rank: 65	Homology Score: 13	TALEN Score: 55.53
	Genomic Region: Intergenic	Closest Gene: ATP8B1
Match Type: R11R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-AGGGC TGCACGTGACTTCTGG                GGCAAAGGTAA                 GTAGGAGAAAGGTGCA CCTTC-3'
	3'-TCCCG ACGTGCACTGAAGACC                CCGTTTCCATT                 CATCCTCTTTCCACGT GGAAG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 018-055555293
Forward Primer: 5'-TCTGGGAAGAAGCTGCCAGG-3' Tm: 59.508
Reverse Primer: 5'-GGCTATGCTACCTGCTGTAAAACC-3' Tm: 59.038
Expected PCR Product Length: 342
Expected CelI Cleavage Product Sizes: 134, 208
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr18:55555404-55555446

Rank: 66	Homology Score: 8	TALEN Score: 55.51
	Genomic Region: Intergenic	Closest Gene: TEAD1
Match Type: R20R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-ATAAC TCCTCCCGACCCCTGT            CCTCAGCATCACCATGGGGT            TCATGATTCAGGTGTA TGCAG-3'
	3'-TATTG AGGAGGGCTGGGGACA            GGAGTCGTAGTGGTACCCCA            AGTACTAAGTCCACAT ACGTC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 011-012966658
Forward Primer: 5'-TGAGGGATAGAGCAGAGGATGG-3' Tm: 59.258
Reverse Primer: 5'-GGGGGAATGATGTCACTGGAAC-3' Tm: 59.258
Expected PCR Product Length: 295
Expected CelI Cleavage Product Sizes: 131, 164
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr11:12966761-12966812

Rank: 67	Homology Score: 5	TALEN Score: 55.5
	Genomic Region: Intergenic	Closest Gene: GPR20
Match Type: R17R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-AGAAG TGTGCCGGACACCTGA             GGAAGGTCCTTCTGCAG              ATGGGAGGCAGGTGTA TCCGC-3'
	3'-TCTTC ACACGGCCTGTGGACT             CCTTCCAGGAAGACGTC              TACCCTCCGTCCACAT AGGCG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 008-142398434
Forward Primer: 5'-GCCTGAGATAGGGACATTGGTG-3' Tm: 59.258
Reverse Primer: 5'-CCCATTCTGAGCGAGCTTGGA-3' Tm: 59.378
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 135, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr8:142398543-142398591

Rank: 68	Homology Score: 5	TALEN Score: 55.49
	Genomic Region: Intergenic	Closest Gene: SH3BP2
Match Type: R21L
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CCCTC TGCCCCTCCCTCCAGT           GCTGGGCTGTGTGGCCAGGGG      ACTGGACTGTGTGGCAGGATGA CTCCA-3'
	3'-GGGAG ACGGGGAGGGAGGTCA           CGACCCGACACACCGGTCCCC      TGACCTGACACACCGTCCTACT GAGGT-5'
	                                                               TGACGGGACACCCCGTTCCACT
	                                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 004-002790263
Forward Primer: 5'-ATGCAGAGATGGCCCAGAGC-3' Tm: 59.508
Reverse Primer: 5'-TGAGTAGGGCCAAGGCCACA-3' Tm: 59.508
Expected PCR Product Length: 375
Expected CelI Cleavage Product Sizes: 237, 138
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr4:2790469-2790527

Rank: 69	Homology Score: 13	TALEN Score: 55.49
	Genomic Region: Intergenic	Closest Gene: CTSB
Match Type: R29R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CTTGG TGCACCTGACTGCCAT       GGGGTGTCCCAGCAAAAGGGCATCAATCT        ACTGGAGCCACATGCA GTCTA-3'
	3'-GAACC ACGTGGACTGACGGTA       CCCCACAGGGTCGTTTTCCCGTAGTTAGA        TGACCTCGGTGTACGT CAGAT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 008-011765812
Forward Primer: 5'-ATCCCCAGTAATGTACCTGCTGTC-3' Tm: 59.038
Reverse Primer: 5'-CTCAGTACACTTGCACCCGG-3' Tm: 59.508
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 134, 136
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr8:11765914-11765974

Rank: 70	Homology Score: 10	TALEN Score: 55.47
	Genomic Region: Intron	Closest Gene: FAM134B
Match Type: R20R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-AAGAT TGCCCCTGACTCAGGT            CCTTGGAAACCCAGAGTTGC            TCAGGATACAGTTGTA AGCAG-3'
	3'-TTCTA ACGGGGACTGAGTCCA            GGAACCTTTGGGTCTCAACG            AGTCCTATGTCAACAT TCGTC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 005-016519335
Forward Primer: 5'-CCTAGATCAGCGGCTTTCAAAGCA-3' Tm: 59.038
Reverse Primer: 5'-GGGCTGAAAGGAGCTTTGAGG-3' Tm: 59.378
Expected PCR Product Length: 374
Expected CelI Cleavage Product Sizes: 189, 185
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr5:16519496-16519547

Rank: 71	Homology Score: 17	TALEN Score: 55.44
	Genomic Region: Intron	Closest Gene: MYLK
Match Type: R12R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-ACTAC TGCACCTGGCTTCTGT                GTTGTGTTTCAA                GCAGCAGACAGATGCC CCGAC-3'
	3'-TGATG ACGTGGACCGAAGACA                CAACACAAAGTT                CGTCGTCTGTCTACGG GGCTG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 003-123352705
Forward Primer: 5'-GGTTTTAAGAAATGGGGGTCTCGC-3' Tm: 59.038
Reverse Primer: 5'-TCCCACCAGAGTATCAGATGGC-3' Tm: 59.258
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 138, 132
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr3:123352819-123352862

Rank: 72	Homology Score: 10	TALEN Score: 55.43
	Genomic Region: Intergenic	Closest Gene: GRXCR1
Match Type: R25R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TTGTA TCCACCTGGCAGCTGG         TATCATGTAGCACCGCTTATCTTGC          AAAAGAGTTAGGTGCA TGGGT-3'
	3'-AACAT AGGTGGACCGTCGACC         ATAGTACATCGTGGCGAATAGAACG          TTTTCTCAATCCACGT ACCCA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 004-043460367
Forward Primer: 5'-GCTAAGAGCCTCTTGGGCTTTTG-3' Tm: 59.148
Reverse Primer: 5'-AGCAGAGGCAGAAAGGACCAG-3' Tm: 59.378
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 136, 134
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr4:43460473-43460529

Rank: 73	Homology Score: 5	TALEN Score: 55.43
	Genomic Region: Intron	Closest Gene: FHIT
Match Type: R20R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CTGCT TACACCTGTCTCCTAA            TAAAATCAACTGCCAATCAC            AGGGGAGGCTGGGGCA TTTTA-3'
	3'-GACGA ATGTGGACAGAGGATT            ATTTTAGTTGACGGTTAGTG            TCCCCTCCGACCCCGT AAAAT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 003-060011649
Forward Primer: 5'-GTACTGCCAGAGTGTCAAGAAAGC-3' Tm: 59.038
Reverse Primer: 5'-TAGGAAGAGGACAGTAGGCTAGG-3' Tm: 59.148
Expected PCR Product Length: 286
Expected CelI Cleavage Product Sizes: 156, 130
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr3:60011777-60011828

Rank: 74	Homology Score: 10	TALEN Score: 55.41
	Genomic Region: Exon	Closest Gene: KIF20A
Match Type: R14R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CATCT TACACCCCTCTCCTTT               GGCCCCAGATCTCA               ACTGGATTCATGTGCA AGATG-3'
	3'-GTAGA ATGTGGGGAGAGGAAA               CCGGGGTCTAGAGT               TGACCTAAGTACACGT TCTAC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 005-137519020
Forward Primer: 5'-GCGAGGATCAAAATGGCAATCCC-3' Tm: 59.148
Reverse Primer: 5'-GGCAGAGGACTAATGTTTCCTGG-3' Tm: 59.148
Expected PCR Product Length: 310
Expected CelI Cleavage Product Sizes: 140, 170
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr5:137519135-137519180

Rank: 75	Homology Score: 17	TALEN Score: 55.41
	Genomic Region: Intergenic	Closest Gene: GRIK2
Match Type: R29R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CTTGG TGCACCCGACTGCTGT       GGGGTGTCCCAGCAGGAGGTTGTCAATGT        ACTGGAGCAACATGCA ACCTA-3'
	3'-GAACC ACGTGGGCTGACGACA       CCCCACAGGGTCGTCCTCCAACAGTTACA        TGACCTCGTTGTACGT TGGAT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 006-101676033
Forward Primer: 5'-CCAGGTAACATACTTGCTGTCTGC-3' Tm: 59.038
Reverse Primer: 5'-GTCACTACTCAGTACACTTGGACC-3' Tm: 59.038
Expected PCR Product Length: 275
Expected CelI Cleavage Product Sizes: 131, 144
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr6:101676132-101676192

Rank: 76	Homology Score: 2	TALEN Score: 55.25
	Genomic Region: Intergenic	Closest Gene: TICRR
Match Type: L30R
	           Left Nuclease
	         TCACCTTGCCCCACAGGGCAGT
	5'-GCCCG TCACCTTGCCCAACCCAACAGT CCATTCTCAGCCTTCATTTTATCCCACCTC       ACAGCAGCCTGTGGCA GAGGG-3'
	3'-CGGGC AGTGGAACGGGTTGGGTTGTCA GGTAAGAGTCGGAAGTAAAATAGGGTGGAG       TGTCGTCGGACACCGT CTCCC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 015-090111045
Forward Primer: 5'-GCCTTCCTCTCCTCATACCC-3' Tm: 59.508
Reverse Primer: 5'-TACAGGTGTGAGCCACCACG-3' Tm: 59.508
Expected PCR Product Length: 273
Expected CelI Cleavage Product Sizes: 131, 142
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr15:90111140-90111207

Rank: 77	Homology Score: 13	TALEN Score: 55.23
	Genomic Region: Intron	Closest Gene: RALGPS1
Match Type: R22R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TGAGC TGCGCCTGAGTCCAGG           CCTTGCTATTCCTGAGATAGAA           AACGGAGTCAGATGCA GAGCT-3'
	3'-ACTCG ACGCGGACTCAGGTCC           GGAACGATAAGGACTCTATCTT           TTGCCTCAGTCTACGT CTCGA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 009-129960176
Forward Primer: 5'-CTTCCTTGGAGTGTCCAATGGG-3' Tm: 59.258
Reverse Primer: 5'-CATCAGGGCTTTTGGCCTCAAAC-3' Tm: 59.148
Expected PCR Product Length: 278
Expected CelI Cleavage Product Sizes: 148, 130
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr9:129960295-129960348

Rank: 78	Homology Score: 10	TALEN Score: 55.11
	Genomic Region: Intergenic	Closest Gene: AFF2
Match Type: R21R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GGATT TGAGCCTGACTTCTCA           GCCCTAATGCTCCTTTACAGG            ACAGGAGCCATGTGAA ATTAA-3'
	3'-CCTAA ACTCGGACTGAAGAGT           CGGGATTACGAGGAAATGTCC            TGTCCTCGGTACACTT TAATT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 00X-148285072
Forward Primer: 5'-GGAGCAAAAAGGACTATGTCACTGAGTCAA-3' Tm: 60.058
Reverse Primer: 5'-CCACGTAAGTCCTACAGGTGACTA-3' Tm: 59.038
Expected PCR Product Length: 450
Expected CelI Cleavage Product Sizes: 307, 143
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chrX:148285351-148285403

Rank: 79	Homology Score: 10	TALEN Score: 55.09
	Genomic Region: Intergenic	Closest Gene: PCDH10
Match Type: R26R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CCAAT TGCATCAGATACCTGA         TTTAGCACACTGATTAAAAGGAATGA         ACTGTAGTCAGGTTCA GTGGC-3'
	3'-GGTTA ACGTAGTCTATGGACT         AAATCGTGTGACTAATTTTCCTTACT         TGACATCAGTCCAAGT CACCG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 004-132113714
Forward Primer: 5'-GCTGTTCTGAGGTTGTTCCTGTG-3' Tm: 59.148
Reverse Primer: 5'-CCAGACTGAAGCATAGTCCCATGA-3' Tm: 59.038
Expected PCR Product Length: 291
Expected CelI Cleavage Product Sizes: 151, 140
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr4:132113834-132113891

Rank: 80	Homology Score: 10	TALEN Score: 55.05
	Genomic Region: Intron	Closest Gene: TBC1D23
Match Type: R23R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TGGAT GGCACATGGCTTCTGA          AAGATCGTGGGGAGTGGGTGGAG           ATAAGAGTCAGGTGAA GTTAC-3'
	3'-ACCTA CCGTGTACCGAAGACT          TTCTAGCACCCCTCACCCACCTC           TATTCTCAGTCCACTT CAATG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 003-099990183
Forward Primer: 5'-GTGGAGACGTTTTTGTGGTGGAC-3' Tm: 59.148
Reverse Primer: 5'-CTCAGCCCCCTCCAAAAACAAAC-3' Tm: 59.148
Expected PCR Product Length: 287
Expected CelI Cleavage Product Sizes: 147, 140
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr3:99990301-99990355

Rank: 81	Homology Score: 8	TALEN Score: 55
	Genomic Region: Intergenic	Closest Gene: PISRT1
Match Type: R11R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GTGAT TACCCCTGACTCCACT                CCTGGATGAGG                 TCAGGGGTCATCTGCA CAGAC-3'
	3'-CACTA ATGGGGACTGAGGTGA                GGACCTACTCC                 AGTCCCCAGTAGACGT GTCTG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 003-138953648
Forward Primer: 5'-CTTGGTGTCTGAGTGGCAAAACTG-3' Tm: 59.038
Reverse Primer: 5'-TATGGGCCTGTTTTACACGGGCCT-3' Tm: 60.908
Expected PCR Product Length: 281
Expected CelI Cleavage Product Sizes: 148, 133
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr3:138953773-138953815

Rank: 82	Homology Score: 10	TALEN Score: 54.93
	Genomic Region: Intergenic	Closest Gene: HAS2
Match Type: R18R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CCTCT TGGACCTGACACCTCT             TCCCCAAACCAGAGGAAC             TCTGTAGCCAGGGGCA TCAAC-3'
	3'-GGAGA ACCTGGACTGTGGAGA             AGGGGTTTGGTCTCCTTG             AGACATCGGTCCCCGT AGTTG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 008-122428182
Forward Primer: 5'-ACAAGTTCTGCCAACTACACCAGG-3' Tm: 59.038
Reverse Primer: 5'-AGAGCCATTCCCAAGTGGGGT-3' Tm: 59.378
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 134, 136
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr8:122428289-122428338

Rank: 83	Homology Score: 10	TALEN Score: 54.88
	Genomic Region: Intergenic	Closest Gene: PTPRT
Match Type: R27R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GGGTC TGCACCAGGCCACTCT        CCTTTCTGAGCACCGAGTGAAGGCATC         ACAGGATCCAGTTGCA GAGCA-3'
	3'-CCCAG ACGTGGTCCGGTGAGA        GGAAAGACTCGTGGCTCACTTCCGTAG         TGTCCTAGGTCAACGT CTCGT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 020-040489952
Forward Primer: 5'-GTGCCACCCACACAGCATTC-3' Tm: 59.508
Reverse Primer: 5'-GCCAGCAATGTAGAGGGACAATG-3' Tm: 59.148
Expected PCR Product Length: 313
Expected CelI Cleavage Product Sizes: 182, 131
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr20:40490103-40490161

Rank: 84	Homology Score: 5	TALEN Score: 54.86
	Genomic Region: Intergenic	Closest Gene: TBX22
Match Type: R28R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TCATC TGCACATAACTACTCT        CCTTTTGAGAAACAGTTCTTGGCCTGTT        ACTGGGGTTTGGTGGA AACTG-3'
	3'-AGTAG ACGTGTATTGATGAGA        GGAAAACTCTTTGTCAAGAACCGGACAA        TGACCCCAAACCACCT TTGAC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 00X-079108050
Forward Primer: 5'-GGTGTCAGTAGCAGATAGGGATG-3' Tm: 59.148
Reverse Primer: 5'-CTGTACCCAACCCACTTTATGGC-3' Tm: 59.148
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 136, 134
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chrX:79108154-79108213

Rank: 85	Homology Score: 10	TALEN Score: 54.85
	Genomic Region: Intergenic	Closest Gene: AIM1
Match Type: R24R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CTCAG TGTCCCAGACTGCAGT          CTATGACTGTGGTGGTATGGTTTT          GCAGGGGTCAGGGGCA CTAAG-3'
	3'-GAGTC ACAGGGTCTGACGTCA          GATACTGACACCACCATACCAAAA          CGTCCCCAGTCCCCGT GATTC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 006-106935880
Forward Primer: 5'-TGGTGCATTGACTTTGGTTCTGGG-3' Tm: 59.038
Reverse Primer: 5'-TCTGACCCAGACCTGACAGATAG-3' Tm: 59.148
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 132, 138
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr6:106935982-106936037

Rank: 86	Homology Score: 10	TALEN Score: 54.85
	Genomic Region: Intron	Closest Gene: TGFBR2
Match Type: R17R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-ATTCC TCCACCTGAGTCCTAT             TGAGGTGCTCCCCATTA              TAAGGAGTCCTGTGAA TCAAA-3'
	3'-TAAGG AGGTGGACTCAGGATA             ACTCCACGAGGGGTAAT              ATTCCTCAGGACACTT AGTTT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 003-030699112
Forward Primer: 5'-CTGGCACAGAGTGAGCACTTAATG-3' Tm: 59.038
Reverse Primer: 5'-GGTCGTCATGAGGAGAACACCTT-3' Tm: 59.148
Expected PCR Product Length: 327
Expected CelI Cleavage Product Sizes: 172, 155
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr3:30699258-30699306

Rank: 87	Homology Score: 10	TALEN Score: 54.83
	Genomic Region: Intron	Closest Gene: GOLGA6B
Match Type: R15R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CTGCC TGCACCTGGCTCATCT              GTTGGCCTTGGCCTG               GAAGGAGCCAGAGGCA GAGGC-3'
	3'-GACGG ACGTGGACCGAGTAGA              CAACCGGAACCGGAC               CTTCCTCGGTCTCCGT CTCCG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 015-072957610
Forward Primer: 5'-TACTCACCTCTTGCCTTCAAGTGG-3' Tm: 59.038
Reverse Primer: 5'-CTTTTCTTGGCCCATGCCAGG-3' Tm: 59.378
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 133, 137
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr15:72957718-72957764

Rank: 88	Homology Score: 10	TALEN Score: 54.83
	Genomic Region: Intron	Closest Gene: GOLGA6A
Match Type: R15R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GCCTC TGCCTCTGGCTCCTTC              CAGGCGAAGGCCAAC               AGATGAGCCAGGTGCA GGCAG-3'
	3'-CGGAG ACGGAGACCGAGGAAG              GTCCGCTTCCGGTTG               TCTACTCGGTCCACGT CCGTC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 015-074364061
Forward Primer: 5'-TTGGCCCATGCCAGGACTCA-3' Tm: 59.508
Reverse Primer: 5'-GCCCCTACAAGAATACTCACCTC-3' Tm: 59.148
Expected PCR Product Length: 277
Expected CelI Cleavage Product Sizes: 134, 143
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr15:74364170-74364216

Rank: 89	Homology Score: 10	TALEN Score: 54.83
	Genomic Region: Intron	Closest Gene: GOLGA6C
Match Type: R15R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CCGCC TGCACCTGGCTCATCT              GGTGGCCTTGGCCTG               GAAGGAGCCAGAGGCA GAGGC-3'
	3'-GGCGG ACGTGGACCGAGTAGA              CCACCGGAACCGGAC               CTTCCTCGGTCTCCGT CTCCG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 015-075561464
Forward Primer: 5'-TACTCACCTCTTGCCTTCAAGTGG-3' Tm: 59.038
Reverse Primer: 5'-TTCTTGGCCCATGCCAGGAC-3' Tm: 59.508
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 133, 137
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr15:75561572-75561618

Rank: 90	Homology Score: 10	TALEN Score: 54.83
	Genomic Region: Intron	Closest Gene: GOLGA6D
Match Type: R15R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CTGCC TGCACCTGGCTCATCT              GGTGGCCTTGGCCTG               GAAGGAGCCAGAGGCA GAGGC-3'
	3'-GACGG ACGTGGACCGAGTAGA              CCACCGGAACCGGAC               CTTCCTCGGTCTCCGT CTCCG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 015-075585740
Forward Primer: 5'-TACTCACCTCTTGCCTTCAAGTGG-3' Tm: 59.038
Reverse Primer: 5'-TTCTTGGCCCATGCCAGGAC-3' Tm: 59.508
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 133, 137
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr15:75585848-75585894

Rank: 91	Homology Score: 10	TALEN Score: 54.83
	Genomic Region: Intergenic	Closest Gene: MIR4313
Match Type: R15R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CCACC TGCACCTGGCTCATCT              GGTGGCCTTGGCCTG               GAAGGAGCCAGAGGCA GAGGC-3'
	3'-GGTGG ACGTGGACCGAGTAGA              CCACCGGAACCGGAC               CTTCCTCGGTCTCCGT CTCCG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 015-076078165
Forward Primer: 5'-TACTCACCTCTTGCCTTCAAGTGG-3' Tm: 59.038
Reverse Primer: 5'-CTTTTCTTGGCCCTTGCCAGGA-3' Tm: 59.258
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 133, 137
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr15:76078273-76078319

Rank: 92	Homology Score: 10	TALEN Score: 54.79
	Genomic Region: Intergenic	Closest Gene: DYM
Match Type: R25R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GGTCA AACTCTTGCCTCCTGT         ATGTAGGAAGGAAGGCCACAGCCAG          ACATGAGGCCGGTGCA GTCAC-3'
	3'-CCAGT TTGAGAACGGAGGACA         TACATCCTTCCTTCCGGTGTCGGTC          TGTACTCCGGCCACGT CAGTG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 018-046555916
Forward Primer: 5'-AAATGCACTTACGGGAGATGGCTG-3' Tm: 59.038
Reverse Primer: 5'-GATGTCTCCCTTTCCCTATCCAAC-3' Tm: 59.038
Expected PCR Product Length: 269
Expected CelI Cleavage Product Sizes: 132, 137
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr18:46556018-46556074

Rank: 93	Homology Score: 10	TALEN Score: 54.79
	Genomic Region: Intergenic	Closest Gene: LRP2
Match Type: R13R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GAACG TGCACCAGAATTCAGC               CAAGCTCCCTATC                AAAGGAGTCAGGTCTA TCTTT-3'
	3'-CTTGC ACGTGGTCTTAAGTCG               GTTCGAGGGATAG                TTTCCTCAGTCCAGAT AGAAA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 002-170227380
Forward Primer: 5'-GGCCAGAAGACCAAGATGATTCC-3' Tm: 59.148
Reverse Primer: 5'-GCCAAAAGCCAAATGTAGACTTTGTGCTG-3' Tm: 60.178
Expected PCR Product Length: 403
Expected CelI Cleavage Product Sizes: 259, 144
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr2:170227615-170227659

Rank: 94	Homology Score: 10	TALEN Score: 54.77
	Genomic Region: Intron	Closest Gene: CDC42BPA
Match Type: R26R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-ACTTC TGCAGCTGACTCAACA         ATCTGGGTTTCAACCACCCTTCCTCC         ATAGGATTCAGATGCA TGCCA-3'
	3'-TGAAG ACGTCGACTGAGTTGT         TAGACCCAAAGTTGGTGGGAAGGAGG         TATCCTAAGTCTACGT ACGGT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 001-227379954
Forward Primer: 5'-GAGATCACTCTCACCACCCAAAAC-3' Tm: 59.038
Reverse Primer: 5'-GGAAACAGCAGAACGTTGTTGGTG-3' Tm: 59.038
Expected PCR Product Length: 383
Expected CelI Cleavage Product Sizes: 190, 193
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr1:227380113-227380170

Rank: 95	Homology Score: 10	TALEN Score: 54.71
	Genomic Region: Intergenic	Closest Gene: PPARGC1A
Match Type: R13R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-ACAAA TGCACCCTACTCATGT               AAGATGTTATAAC                AGGGGAGACTGGGGCA GGGCA-3'
	3'-TGTTT ACGTGGGATGAGTACA               TTCTACAATATTG                TCCCCTCTGACCCCGT CCCGT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 004-024170953
Forward Primer: 5'-CTGGATGGGATCCCAGAACAGA-3' Tm: 59.258
Reverse Primer: 5'-GAGCCAACATGCTTGGCTGAG-3' Tm: 59.378
Expected PCR Product Length: 276
Expected CelI Cleavage Product Sizes: 140, 136
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr4:24171069-24171113

Rank: 96	Homology Score: 10	TALEN Score: 54.7
	Genomic Region: Intron	Closest Gene: LINC00299
Match Type: R25R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GCTTT TGCACCTGCCCCCAGT         GCTGTAAGTTCTATGGAAATTCCAC          CCTGGTGTCAGCTCCA CACTG-3'
	3'-CGAAA ACGTGGACGGGGGTCA         CGACATTCAAGATACCTTTAAGGTG          GGACCACAGTCGAGGT GTGAC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 002-008374518
Forward Primer: 5'-TCCCGGAAACATGTCAGCCC-3' Tm: 59.508
Reverse Primer: 5'-CATCCTACATCCCCTGAACATGG-3' Tm: 59.148
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 139, 131
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr2:8374627-8374683

Rank: 97	Homology Score: 10	TALEN Score: 54.7
	Genomic Region: Intergenic	Closest Gene: IARS
Match Type: R23R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-ACCCC CGCACCTCACTCCAGT          ATCCCAGGGAGAGAGATTCTCAG           GCAGGAGTCTGTTGTG CTGTG-3'
	3'-TGGGG GCGTGGAGTGAGGTCA          TAGGGTCCCTCTCTCTAAGAGTC           CGTCCTCAGACAACAC GACAC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 009-094951264
Forward Primer: 5'-ATCCAGCCCTGGGACTTTACTTC-3' Tm: 59.148
Reverse Primer: 5'-TGTGAGTCTCAGAACCAAGGCTGT-3' Tm: 59.038
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 135, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr9:94951370-94951424

Rank: 98	Homology Score: 10	TALEN Score: 54.68
	Genomic Region: Intron	Closest Gene: BDNF-AS
Match Type: R12R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TCTGA TACCTCTGACTCCTGT                TTACCTAGAGGA                AGAAGAATCTGGAGCA GCAGT-3'
	3'-AGACT ATGGAGACTGAGGACA                AATGGATCTCCT                TCTTCTTAGACCTCGT CGTCA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 011-027668392
Forward Primer: 5'-GGTGGGTCCATTCTGACTCTG-3' Tm: 59.378
Reverse Primer: 5'-TCATGATCCACCCACCTCGG-3' Tm: 59.508
Expected PCR Product Length: 362
Expected CelI Cleavage Product Sizes: 219, 143
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr11:27668587-27668630

Rank: 99	Homology Score: 10	TALEN Score: 54.68
	Genomic Region: Intergenic	Closest Gene: SALL3
Match Type: R16R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CACTC TGCACCTGCAGCCTCC              TCCTGATGGATGACCA              ACAGGAGCAAGGGGCA AGGCA-3'
	3'-GTGAG ACGTGGACGTCGGAGG              AGGACTACCTACTGGT              TGTCCTCGTTCCCCGT TCCGT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 018-076246512
Forward Primer: 5'-AGCCTCCTACTGATGGGTGG-3' Tm: 59.508
Reverse Primer: 5'-GCTGATGAAGAAGAGGATGGGG-3' Tm: 59.258
Expected PCR Product Length: 286
Expected CelI Cleavage Product Sizes: 151, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr18:76246637-76246684

Rank: 100	Homology Score: 10	TALEN Score: 54.68
	Genomic Region: Intergenic	Closest Gene: SALL3
Match Type: R10R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GCACC TGCAGCCTCCTCCTGA                 TGGATGACCA                 ACAGGAGCAAGGGGCA AGGCA-3'
	3'-CGTGG ACGTCGGAGGAGGACT                 ACCTACTGGT                 TGTCCTCGTTCCCCGT TCCGT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 018-076246512
Forward Primer: 5'-AGCCTCCTACTGATGGGTGG-3' Tm: 59.508
Reverse Primer: 5'-GCTGATGAAGAAGAGGATGGGG-3' Tm: 59.258
Expected PCR Product Length: 286
Expected CelI Cleavage Product Sizes: 154, 132
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr18:76246643-76246684

Rank: 101	Homology Score: 8	TALEN Score: 54.67
	Genomic Region: Intergenic	Closest Gene: MIR4790
Match Type: R19R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GGATT TGAACCTGGCTTCTCT            GCCACCTGCATTTTTGACC             ATGGGAGCCAGGAGCA GGGCG-3'
	3'-CCTAA ACTTGGACCGAAGAGA            CGGTGGACGTAAAAACTGG             TACCCTCGGTCCTCGT CCCGC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 003-005393709
Forward Primer: 5'-CATCTACAGAATAACCCCAGGAGG-3' Tm: 59.038
Reverse Primer: 5'-CCGTGTCACAGACAGTGTCTGAA-3' Tm: 59.148
Expected PCR Product Length: 310
Expected CelI Cleavage Product Sizes: 140, 170
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr3:5393822-5393872

Rank: 102	Homology Score: 10	TALEN Score: 54.62
	Genomic Region: Intergenic	Closest Gene: TNFSF15
Match Type: R20R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-ATCAC CCCAGCAGACCCCTGT            GGGTTTGCTCACATAAGGGA            ACAGGAGTTATGAGCA ATGAA-3'
	3'-TAGTG GGGTCGTCTGGGGACA            CCCAAACGAGTGTATTCCCT            TGTCCTCAATACTCGT TACTT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 009-117519299
Forward Primer: 5'-CAAAAGGACCTGCATCTAAGCACC-3' Tm: 59.038
Reverse Primer: 5'-ATTGTTCTTCCCTGGCCACCC-3' Tm: 59.378
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 135, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr9:117519406-117519457

Rank: 103	Homology Score: 10	TALEN Score: 54.61
	Genomic Region: Intergenic	Closest Gene: COG2
Match Type: R28R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TTATT TGAACATGACTTCTGT        GCATTCCATTGCACCATGTGGTGGGGAG        AAAGGTGTCTGGTCTA TTTTT-3'
	3'-AATAA ACTTGTACTGAAGACA        CGTAAGGTAACGTGGTACACCACCCCTC        TTTCCACAGACCAGAT AAAAA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 001-230749531
Forward Primer: 5'-CAAAGATGCAAAGGCTGATTTAGAGCACC-3' Tm: 60.178
Reverse Primer: 5'-CCCCAAATTTAGCAATACTGTCTGCTGGA-3' Tm: 60.178
Expected PCR Product Length: 441
Expected CelI Cleavage Product Sizes: 131, 310
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr1:230749630-230749689

Rank: 104	Homology Score: 5	TALEN Score: 54.61
	Genomic Region: Intron	Closest Gene: ROCK2
Match Type: R10R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-ACTAC TGCACCTAACTCACAC                 AGAAAAGAGC                 AGAGGAGGTAGGAGCA ATTTA-3'
	3'-TGATG ACGTGGATTGAGTGTG                 TCTTTTCTCG                 TCTCCTCCATCCTCGT TAAAT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Warning! Primer pair was generated using suboptimal parameters. 
Primer Index: 002-011376315
Forward Primer: 5'-CCCCTCATGGAGAACACAATACAAAAGAAAC-3' Tm: 59.958
Reverse Primer: 5'-GAGAGAAGAGTTACGCGTCTTCC-3' Tm: 59.148
Expected PCR Product Length: 307
Expected CelI Cleavage Product Sizes: 169, 138
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr2:11376461-11376502

Rank: 105	Homology Score: 10	TALEN Score: 54.59
	Genomic Region: Intron	Closest Gene: EIF2B3
Match Type: R12R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-AAAAA TGCAGCTCACACCTGT                GATCCCAGTACT                TTAGGAGGCAGAGGCA GGCAG-3'
	3'-TTTTT ACGTCGAGTGTGGACA                CTAGGGTCATGA                AATCCTCCGTCTCCGT CCGTC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 001-045422945
Forward Primer: 5'-GCCCCCAAACACCACAAAGTC-3' Tm: 59.378
Reverse Primer: 5'-ATTACAGGCACATGCCACCACG-3' Tm: 59.258
Expected PCR Product Length: 370
Expected CelI Cleavage Product Sizes: 240, 130
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr1:45423161-45423204

Rank: 106	Homology Score: 10	TALEN Score: 54.56
	Genomic Region: Intron	Closest Gene: JAKMIP3
Match Type: R16R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-AGGGG TGCACCTCACAACTGT              GGGAGCAGGTGATGTG              GCATGAGGCAGGGGCG GGGCT-3'
	3'-TCCCC ACGTGGAGTGTTGACA              CCCTCGTCCACTACAC              CGTACTCCGTCCCCGC CCCGA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 010-133959783
Forward Primer: 5'-TTTCTCTCGGCACCTCCCAC-3' Tm: 59.508
Reverse Primer: 5'-GTCCCTGCCCATGGACAAGT-3' Tm: 59.508
Expected PCR Product Length: 287
Expected CelI Cleavage Product Sizes: 157, 130
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr10:133959914-133959961

Rank: 107	Homology Score: 5	TALEN Score: 54.54
	Genomic Region: Intron	Closest Gene: ROBO2
Match Type: R21R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-ACAGA AACACCTGAAACCGGT           GATCAGAAACTTCTGAATATC            TCAGGAGTTGGGTGAA TGGGC-3'
	3'-TGTCT TTGTGGACTTTGGCCA           CTAGTCTTTGAAGACTTATAG            AGTCCTCAACCCACTT ACCCG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 003-077451553
Forward Primer: 5'-CTGCACGTGTGTCCCAGAAC-3' Tm: 59.508
Reverse Primer: 5'-ACACACGCTCACTTGAGGCATTC-3' Tm: 59.148
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 137, 133
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr3:77451662-77451714

Rank: 108	Homology Score: 5	TALEN Score: 54.44
	Genomic Region: Intron	Closest Gene: GRM8
Match Type: R25R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GCCAC TGCACCTGGTCCAGGT         TTGTTATACAGGTAAAGGGTATGTC          ACAGGGGTTTGGTGTA CATAT-3'
	3'-CGGTG ACGTGGACCAGGTCCA         AACAATATGTCCATTTCCCATACAG          TGTCCCCAAACCACAT GTATA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 007-126235510
Forward Primer: 5'-GGGGTTTTGTCATGTTGCCCG-3' Tm: 59.378
Reverse Primer: 5'-GCGACCAGCCGGACTATAAATC-3' Tm: 59.258
Expected PCR Product Length: 410
Expected CelI Cleavage Product Sizes: 130, 280
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr7:126235610-126235666

Rank: 109	Homology Score: 10	TALEN Score: 54.42
	Genomic Region: Intron	Closest Gene: MS4A4A
Match Type: R22R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TGACC AGAACCTGACCTATGT           TACCAAAGAATTATGGGAAAGC           ATAGGAGTCAGTTACA AGCTT-3'
	3'-ACTGG TCTTGGACTGGATACA           ATGGTTTCTTAATACCCTTTCG           TATCCTCAGTCAATGT TCGAA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 011-060049460
Forward Primer: 5'-GGGGACTTCATGTGCCGTAG-3' Tm: 59.508
Reverse Primer: 5'-GGTCTGTCTTATCAAACGCACTGG-3' Tm: 59.038
Expected PCR Product Length: 281
Expected CelI Cleavage Product Sizes: 150, 131
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr11:60049581-60049634

Rank: 110	Homology Score: 10	TALEN Score: 54.4
	Genomic Region: Intergenic	Closest Gene: LINC00290
Match Type: R30R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GCTTC TGCTCCGAAGTCCTGC       ATGGACATGCTTGTATATATTTATACCATT       AAAGGAGTCAGTTTCA AAAAT-3'
	3'-CGAAG ACGAGGCTTCAGGACG       TACCTGTACGAACATATATAAATATGGTAA       TTTCCTCAGTCAAAGT TTTTA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 004-182299969
Forward Primer: 5'-GACAAGGAAACTGAGGCACAGAAG-3' Tm: 59.038
Reverse Primer: 5'-ACACTGATTCCTGACCTCAGCG-3' Tm: 59.258
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 138, 132
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr4:182300074-182300135

Rank: 111	Homology Score: 5	TALEN Score: 54.39
	Genomic Region: Intron	Closest Gene: OR8U8
Match Type: L17R
	           Left Nuclease
	         TCACCTTGCCCCACAGGGCAGT
	5'-ATGTC TCGCCTCCCGCCACAGGGCGGT       TTTTCTCCTATCTCAGA              ATAGAAGAAAGGTGCA ATCAG-3'
	3'-TACAG AGCGGAGGGCGGTGTCCCGCCA       AAAAGAGGATAGAGTCT              TATCTTCTTTCCACGT TAGTC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 011-056386094
Forward Primer: 5'-GGGAGGTACTATGCCTGGATG-3' Tm: 59.378
Reverse Primer: 5'-GCTCGGGAGGATTAGTAAAAGAGG-3' Tm: 59.038
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 136, 134
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr11:56386201-56386255

Rank: 112	Homology Score: 5	TALEN Score: 54.37
	Genomic Region: Intron	Closest Gene: TEX11
Match Type: R28R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CCTGG GTCTCCTGCCTCCTGA        GGAGATGGATAAAGATGGGCTTCCTCTC        ATGGGAGTCAGGCGTA GACTT-3'
	3'-GGACC CAGAGGACGGAGGACT        CCTCTACCTATTTCTACCCGAAGGAGAG        TACCCTCAGTCCGCAT CTGAA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 00X-070076863
Forward Primer: 5'-CAGGTGTGGTAAAGTGAACTGGG-3' Tm: 59.148
Reverse Primer: 5'-GACACTAGTGGCACTTGAAGGAG-3' Tm: 59.148
Expected PCR Product Length: 434
Expected CelI Cleavage Product Sizes: 163, 271
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chrX:70076994-70077053

Rank: 113	Homology Score: 10	TALEN Score: 54.35
	Genomic Region: Intron	Closest Gene: ANK1
Match Type: R15R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TATAA TGAACCTGGCTCCCGT              TCCTTTTATATAAGT               AGGGGAGTGAGGAGGA GGGGA-3'
	3'-ATATT ACTTGGACCGAGGGCA              AGGAAAATATATTCA               TCCCCTCACTCCTCCT CCCCT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 008-041570215
Forward Primer: 5'-GGGTGCAGAGAGATTTGCATGTG-3' Tm: 59.148
Reverse Primer: 5'-TGTCTCCTCCTGTCCCTGCT-3' Tm: 59.508
Expected PCR Product Length: 289
Expected CelI Cleavage Product Sizes: 153, 136
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr8:41570343-41570389

Rank: 114	Homology Score: 10	TALEN Score: 54.34
	Genomic Region: Intergenic	Closest Gene: MAN1A2
Match Type: R11R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-ATCTT TGACCCTGCCTCCTGT                CTTTATTTCAA                 AAAGGATGCAATTGCA AAGAT-3'
	3'-TAGAA ACTGGGACGGAGGACA                GAAATAAAGTT                 TTTCCTACGTTAACGT TTCTA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Warning! Primer pair was generated using suboptimal parameters. 
Primer Index: 001-118086389
Forward Primer: 5'-CTGTTCATTTTCTTAAGGTGGAGACTTACCTG-3' Tm: 59.848
Reverse Primer: 5'-GCACTCTGGAAACATTCTCTTTCAATGTGTC-3' Tm: 59.958
Expected PCR Product Length: 448
Expected CelI Cleavage Product Sizes: 270, 178
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr1:118086636-118086678

Rank: 115	Homology Score: 5	TALEN Score: 54.33
	Genomic Region: Intergenic	Closest Gene: AKIRIN2
Match Type: R25R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CTGTG TGCACAGAACTCCTGG         GGGGGAGATGGAATAAGGAAAGCCA          ATGTGATTCAGGTGTA GTGAG-3'
	3'-GACAC ACGTGTCTTGAGGACC         CCCCCTCTACCTTATTCCTTTCGGT          TACACTAAGTCCACAT CACTC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 006-088451104
Forward Primer: 5'-GATCTCCTTGAAGGCACATGCAG-3' Tm: 59.148
Reverse Primer: 5'-GGCCTGGGAAACATAATGACACC-3' Tm: 59.148
Expected PCR Product Length: 325
Expected CelI Cleavage Product Sizes: 130, 195
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr6:88451204-88451260

Rank: 116	Homology Score: 10	TALEN Score: 54.33
	Genomic Region: Intergenic	Closest Gene: GOT2
Match Type: R11R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CTGCC TGCACCCCACTCCTGC                TTGTCCAAATC                 AAATGAGACAGGCTCA ATTAA-3'
	3'-GACGG ACGTGGGGTGAGGACG                AACAGGTTTAG                 TTTACTCTGTCCGAGT TAATT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 016-059193695
Forward Primer: 5'-GTAGCCTCTCCCAATACTTAACGG-3' Tm: 59.038
Reverse Primer: 5'-CCTGTGTTGTTTCTGTCCAAGGC-3' Tm: 59.148
Expected PCR Product Length: 332
Expected CelI Cleavage Product Sizes: 130, 202
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr16:59193802-59193844

Rank: 117	Homology Score: 10	TALEN Score: 54.31
	Genomic Region: Intron	Closest Gene: RBFOX1
Match Type: R12R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GTGGT GGCACCAGACACCTGT                AATCTCAAGTAC                TCAGGAGGCTGAGGCA GGGGA-3'
	3'-CACCA CCGTGGTCTGTGGACA                TTAGAGTTCATG                AGTCCTCCGACTCCGT CCCCT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 016-007614298
Forward Primer: 5'-TTGGGACGCAGAGGTGGGTA-3' Tm: 59.508
Reverse Primer: 5'-CTGGCTTGCAGATGAGTGCCT-3' Tm: 59.378
Expected PCR Product Length: 293
Expected CelI Cleavage Product Sizes: 132, 161
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr16:7614406-7614449

Rank: 118	Homology Score: 10	TALEN Score: 54.26
	Genomic Region: Intron	Closest Gene: LOC100507254
Match Type: R28R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-ATCAC TGGAACTGGTTCCAGT        GCGCATATATACTCTGATCAGCTTTTAC        ACAGGAGTCTGTGGCA GAGAA-3'
	3'-TAGTG ACCTTGACCAAGGTCA        CGCGTATATATGAGACTAGTCGAAAATG        TGTCCTCAGACACCGT CTCTT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 006-132465014
Forward Primer: 5'-GTTGTGTTGTGGCTGCTCAGAGT-3' Tm: 59.148
Reverse Primer: 5'-CCTTCTATCCCCATCCTCTCTTTC-3' Tm: 59.038
Expected PCR Product Length: 293
Expected CelI Cleavage Product Sizes: 136, 157
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr6:132465118-132465177

Rank: 119	Homology Score: 10	TALEN Score: 54.25
	Genomic Region: Intergenic	Closest Gene: FOXK1
Match Type: R20R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GCATC AGAACTTCAATCCTGT            TAATTGCCAAATAATATGCC            ACTGTAGCCAGGTGCA GTGGC-3'
	3'-CGTAG TCTTGAAGTTAGGACA            ATTAACGGTTTATTATACGG            TGACATCGGTCCACGT CACCG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 007-004703855
Forward Primer: 5'-CTGCTGTCTCTATGGATTTGCCTG-3' Tm: 59.038
Reverse Primer: 5'-CCACCTCCACACTTGGCCAAT-3' Tm: 59.378
Expected PCR Product Length: 326
Expected CelI Cleavage Product Sizes: 160, 166
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr7:4703987-4704038

Rank: 120	Homology Score: 10	TALEN Score: 54.22
	Genomic Region: Intron	Closest Gene: ZNF676
Match Type: R15R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CCTAC TGCCGCTGTCTCCTTC              TTATTTTGTCAGACA               ACAGGAGTCAGGTTTT GAGTC-3'
	3'-GGATG ACGGCGACAGAGGAAG              AATAAAACAGTCTGT               TGTCCTCAGTCCAAAA CTCAG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 019-022376173
Forward Primer: 5'-GGTACTCTCACTGTGGAAAACTGG-3' Tm: 59.038
Reverse Primer: 5'-GGCCAATTCTCCTCTCCTTTTTCC-3' Tm: 59.038
Expected PCR Product Length: 287
Expected CelI Cleavage Product Sizes: 146, 141
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr19:22376294-22376340

Rank: 121	Homology Score: 8	TALEN Score: 54.22
	Genomic Region: Intergenic	Closest Gene: CA5A
Match Type: R11R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TGTCC TTCAGCAGACTCCTCT                TGATCGATGGA                 ATCGAAGTCAGGCGCA GCAGC-3'
	3'-ACAGG AAGTCGTCTGAGGAGA                ACTAGCTACCT                 TAGCTTCAGTCCGCGT CGTCG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 016-087916217
Forward Primer: 5'-TGGGCCCAACATGTCAGTGC-3' Tm: 59.508
Reverse Primer: 5'-GAGATCACACTTGGTGGCGATG-3' Tm: 59.258
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 135, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr16:87916329-87916371

Rank: 122	Homology Score: 5	TALEN Score: 54.21
	Genomic Region: Intergenic	Closest Gene: PTCHD4
Match Type: R11R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TCAAA TACCCCTCACTCCTAT                TATTAACATTG                 ACAAGAGCATGGTACA TGTAT-3'
	3'-AGTTT ATGGGGAGTGAGGATA                ATAATTGTAAC                 TGTTCTCGTACCATGT ACATA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Warning! Primer pair was generated using suboptimal parameters. 
Primer Index: 006-048618042
Forward Primer: 5'-CCTCCTGTCTTTATTAGTCTAGCAAGAAGTTTGT-3' Tm: 59.668
Reverse Primer: 5'-GTGGTGTCCTGGATAAAATCCTGAAACAGA-3' Tm: 60.058
Expected PCR Product Length: 430
Expected CelI Cleavage Product Sizes: 280, 150
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr6:48618299-48618341

Rank: 123	Homology Score: 13	TALEN Score: 54.19
	Genomic Region: Intron	Closest Gene: RNF103-CHMP3
Match Type: R19R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-AGTGA TCCTCCTGCCTCCTGT            CCCCCAAAATGCTGGGATT             ACAGGTGTGAGCTGCC ATGAC-3'
	3'-TCACT AGGAGGACGGAGGACA            GGGGGTTTTACGACCCTAA             TGTCCACACTCGACGG TACTG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Warning! Primer pair was generated using suboptimal parameters. 
Primer Index: 002-086899302
Forward Primer: 5'-GGCTCTGTCTTTTGTTATGTCAAATGAGAAGCA-3' Tm: 59.758
Reverse Primer: 5'-CTCCATCATTAATTCAAACAGCCTTCAATACCC-3' Tm: 59.758
Expected PCR Product Length: 373
Expected CelI Cleavage Product Sizes: 235, 138
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr2:86899510-86899560

Rank: 124	Homology Score: 10	TALEN Score: 54.19
	Genomic Region: Intron	Closest Gene: RERE
Match Type: R16R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GGTGC TGGACCTGACCCCTAT              CTGCAGTAGCAAGAGA              GCAGAAGACAGGGGCT AAGCT-3'
	3'-CCACG ACCTGGACTGGGGATA              GACGTCATCGTTCTCT              CGTCTTCTGTCCCCGA TTCGA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 001-008489463
Forward Primer: 5'-GGATAGAGAATACTGGGCTGAGAG-3' Tm: 59.038
Reverse Primer: 5'-GTAAGAGGACTGAGTAGATGTCCC-3' Tm: 59.038
Expected PCR Product Length: 308
Expected CelI Cleavage Product Sizes: 137, 171
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr1:8489574-8489621

Rank: 125	Homology Score: 13	TALEN Score: 54.15
	Genomic Region: Intergenic	Closest Gene: FAM69C
Match Type: R26R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CCTAG TTCACTTCACTCCTGT         TATCCACTAAGCTCATTATCACATCG         CCAGAAGTGAGGTCCA TTTGT-3'
	3'-GGATC AAGTGAAGTGAGGACA         ATAGGTGATTCGAGTAATAGTGTAGC         GGTCTTCACTCCAGGT AAACA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 018-072086920
Forward Primer: 5'-GCTGTCACCAGGAAAAACATAAGAGCC-3' Tm: 60.438
Reverse Primer: 5'-AAGCTGCTGAAATGGGCAGAGG-3' Tm: 59.258
Expected PCR Product Length: 289
Expected CelI Cleavage Product Sizes: 159, 130
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr18:72087048-72087105

Rank: 126	Homology Score: 8	TALEN Score: 54.14
	Genomic Region: Intron	Closest Gene: VASH2
Match Type: R12R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CATTT TTCACCAGACTCCCGG                CTCTTCAGCCTG                AGAGGAATATGGTGCA GAGAA-3'
	3'-GTAAA AAGTGGTCTGAGGGCC                GAGAAGTCGGAC                TCTCCTTATACCACGT CTCTT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 001-213140398
Forward Primer: 5'-ATTCTAAGAGTGGCGTGTGGAGG-3' Tm: 59.148
Reverse Primer: 5'-GTTGTGGGTGATTCCAATGGCATG-3' Tm: 59.038
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 134, 136
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr1:213140508-213140551

Rank: 127	Homology Score: 10	TALEN Score: 54.13
	Genomic Region: Exon	Closest Gene: AMIGO2
Match Type: R15R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GCAGA AGTACCTGACGCGAGT              GCCTGGAGTCAGACC               ACAGGCGACAGGTGTA ATCGT-3'
	3'-CGTCT TCATGGACTGCGCTCA              CGGACCTCAGTCTGG               TGTCCGCTGTCCACAT TAGCA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 012-047471859
Forward Primer: 5'-GTGGACCATCAGTCTTTCCCC-3' Tm: 59.378
Reverse Primer: 5'-CTGAGAGGCATCTACCTTCATGG-3' Tm: 59.148
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 134, 136
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr12:47471968-47472014

Rank: 128	Homology Score: 8	TALEN Score: 54.13
	Genomic Region: Intron	Closest Gene: MYBPHL
Match Type: R30R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-ATGTT TTCACATGCTTCCTGT       GATACTCAGTCCCAGAGCTCTCTGTAAGGA       AGAGGAGTTAGGAGGA AGAAA-3'
	3'-TACAA AAGTGTACGAAGGACA       CTATGAGTCAGGGTCTCGAGAGACATTCCT       TCTCCTCAATCCTCCT TCTTT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 001-109847447
Forward Primer: 5'-GCAGGCTTGCCAAACTAAAAGCTC-3' Tm: 59.038
Reverse Primer: 5'-TCAGAGAGCCAACAGAGCCC-3' Tm: 59.508
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 133, 137
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr1:109847547-109847608

Rank: 129	Homology Score: 5	TALEN Score: 54.13
	Genomic Region: Intergenic	Closest Gene: SORCS1
Match Type: R15R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TAATA TGCACCAGACTTCTAG              TATTAAATATTGGGT               AAATGAGTAATGTGTA CCCAT-3'
	3'-ATTAT ACGTGGTCTGAAGATC              ATAATTTATAACCCA               TTTACTCATTACACAT GGGTA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 010-107873570
Forward Primer: 5'-GACACCTTCTGCTAAGCACACATC-3' Tm: 59.038
Reverse Primer: 5'-GAGCTCATTCAAAGCCCCAGTAG-3' Tm: 59.148
Expected PCR Product Length: 358
Expected CelI Cleavage Product Sizes: 183, 175
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr10:107873728-107873774

Rank: 130	Homology Score: 8	TALEN Score: 54.08
	Genomic Region: Intron	Closest Gene: C1orf127
Match Type: R23R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GCATC TGCAGCTACCACCTGT          CTCCCTGGGCCACGTCCTGTGGT           GCAGGAGCCAGGGCCA CCTGC-3'
	3'-CGTAG ACGTCGATGGTGGACA          GAGGGACCCGGTGCAGGACACCA           CGTCCTCGGTCCCGGT GGACG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 001-011023947
Forward Primer: 5'-TTTAATCCCCCCTCCCCTCC-3' Tm: 59.508
Reverse Primer: 5'-AAAGCGTCCACTGTGGGCCCAT-3' Tm: 61.288
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 137, 133
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr1:11024055-11024109

Rank: 131	Homology Score: 8	TALEN Score: 54.06
	Genomic Region: Intergenic	Closest Gene: HSBP1
Match Type: R14R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CGGTA CACACCACACTCCTGT               CTCTCGATGCCATT               GGAGGAGGCAGATGCA TCCGG-3'
	3'-GCCAT GTGTGGTGTGAGGACA               GAGAGCTACGGTAA               CCTCCTCCGTCTACGT AGGCC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 016-083864892
Forward Primer: 5'-GGAAACTGTGGCCCAGAGAG-3' Tm: 59.508
Reverse Primer: 5'-CTCAGCATCCGTTCCTGTTCTTC-3' Tm: 59.148
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 135, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr16:83865002-83865047

Rank: 132	Homology Score: 10	TALEN Score: 54.06
	Genomic Region: Intergenic	Closest Gene: EDIL3
Match Type: R12R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GTCCA TGTACCTAACTCCTTT                GTCTTCAGCATG                AGAGAACACAGGGGCA TACTG-3'
	3'-CAGGT ACATGGATTGAGGAAA                CAGAAGTCGTAC                TCTCTTGTGTCCCCGT ATGAC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 005-083704636
Forward Primer: 5'-GGAACGGATTAAAGGGAAGCCAG-3' Tm: 59.148
Reverse Primer: 5'-GGTCTGTTTCTGTAACATCAGAGGCAAAG-3' Tm: 60.178
Expected PCR Product Length: 361
Expected CelI Cleavage Product Sizes: 133, 228
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr5:83704745-83704788

Rank: 133	Homology Score: 5	TALEN Score: 54.03
	Genomic Region: Intron	Closest Gene: PRKG1
Match Type: R10R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GGATC TGTACCTTCCTCATAT                 GAACATAATG                 ATAGTAGTCATGTTCA TTAAG-3'
	3'-CCTAG ACATGGAAGGAGTATA                 CTTGTATTAC                 TATCATCAGTACAAGT AATTC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 010-052784032
Forward Primer: 5'-ACTGAGGCCCAAATCATAGCCAATGATGGA-3' Tm: 61.548
Reverse Primer: 5'-AGGCCTCCACAGCCATGCTT-3' Tm: 59.508
Expected PCR Product Length: 416
Expected CelI Cleavage Product Sizes: 131, 285
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr10:52784140-52784181

Rank: 134	Homology Score: 10	TALEN Score: 54.02
	Genomic Region: Intron	Closest Gene: PRMT7
Match Type: R22R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GGAAA TGCACGTGGCTCCTGC           TTGTCATCCCAGCACTCTGGGA           ACCTGAGGCTGGTGGA TTGCT-3'
	3'-CCTTT ACGTGCACCGAGGACG           AACAGTAGGGTCGTGAGACCCT           TGGACTCCGACCACCT AACGA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 016-068387900
Forward Primer: 5'-GCGCCAGTGGTCCTTGGATT-3' Tm: 59.508
Reverse Primer: 5'-CTGGGAATACAGGTGTGCACTAC-3' Tm: 59.148
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 134, 136
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr16:68388005-68388058

Rank: 135	Homology Score: 10	TALEN Score: 54.01
	Genomic Region: Intergenic	Closest Gene: ARSB
Match Type: R11R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CTGGC TCCACCTGGCTCCTGC                TTTCACAGCTC                 TCGAGAGCCAGGTGCC AGTCA-3'
	3'-GACCG AGGTGGACCGAGGACG                AAAGTGTCGAG                 AGCTCTCGGTCCACGG TCAGT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 005-078059903
Forward Primer: 5'-CTGCTCAGCTCATCAGAACACTC-3' Tm: 59.148
Reverse Primer: 5'-GGACAGAAGCTTGTTGAGTGGTG-3' Tm: 59.148
Expected PCR Product Length: 346
Expected CelI Cleavage Product Sizes: 202, 144
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr5:78060082-78060124

Rank: 136	Homology Score: 8	TALEN Score: 53.97
	Genomic Region: Intergenic	Closest Gene: CDH18
Match Type: R16R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GCAGT TTCCCCTGCCTCTTGT              ATCACTCCTTGGTCCT              ACAGGATACAAGTGTA GCATA-3'
	3'-CGTCA AAGGGGACGGAGAACA              TAGTGAGGAACCAGGA              TGTCCTATGTTCACAT CGTAT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 005-019467940
Forward Primer: 5'-GAGATCAGGCCACAGTCATATTCC-3' Tm: 59.038
Reverse Primer: 5'-CCTGGCGACAGTTGTCATCATC-3' Tm: 59.258
Expected PCR Product Length: 435
Expected CelI Cleavage Product Sizes: 234, 201
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr5:19468148-19468195

Rank: 137	Homology Score: 10	TALEN Score: 53.97
	Genomic Region: Intron	Closest Gene: LDB2
Match Type: R18R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CTACG AGCACGTGACTCCTAT             AGGTGCAAGCTTTAGAAG             GTAGGAGTCAAGGCCA GGCAC-3'
	3'-GATGC TCGTGCACTGAGGATA             TCCACGTTCGAAATCTTC             CATCCTCAGTTCCGGT CCGTG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 004-016836013
Forward Primer: 5'-CACCTATGTGGCGTGATTGAGTGA-3' Tm: 59.038
Reverse Primer: 5'-AGACAGGGTTTCAGCATGTTGGC-3' Tm: 59.148
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 139, 131
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr4:16836125-16836174

Rank: 138	Homology Score: 8	TALEN Score: 53.9
	Genomic Region: Intron	Closest Gene: TPD52L2
Match Type: R15R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GGAAG TGCACACGGCTCCAGT              CCTATGGGACATTTG               AAGGGAGTGAGATGCA GCCAG-3'
	3'-CCTTC ACGTGTGCCGAGGTCA              GGATACCCTGTAAAC               TTCCCTCACTCTACGT CGGTC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 020-062498891
Forward Primer: 5'-CCTGGGTGACAGAGTAAGACTCG-3' Tm: 61.088
Reverse Primer: 5'-CCAATATGGTGAAACCCCGTCTC-3' Tm: 59.148
Expected PCR Product Length: 307
Expected CelI Cleavage Product Sizes: 130, 177
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr20:62498996-62499042

Rank: 139	Homology Score: 5	TALEN Score: 53.88
	Genomic Region: Intergenic	Closest Gene: NKX6-1
Match Type: R29R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CAATT TTAACCTGACTCCATT       TTTCACTTAGCATTGTTCTAAACAATAAA        ACATGAGTTTAGTGTA TTTAT-3'
	3'-GTTAA AATTGGACTGAGGTAA       AAAGTGAATCGTAACAAGATTTGTTATTT        TGTACTCAAATCACAT AAATA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 004-085199195
Forward Primer: 5'-CAGTTCTACTTCAGGGAAGGGG-3' Tm: 59.258
Reverse Primer: 5'-GGATACAAGTGGAAAAGCACAGCC-3' Tm: 59.038
Expected PCR Product Length: 364
Expected CelI Cleavage Product Sizes: 196, 168
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr4:85199359-85199419

Rank: 140	Homology Score: 8	TALEN Score: 53.87
	Genomic Region: Intergenic	Closest Gene: YIPF5
Match Type: R12R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-ACTGC TTCACCTGTCTCCTCC                CATTATAAATCT                TCAGGAGTCCTGTGGA GGTGG-3'
	3'-TGACG AAGTGGACAGAGGAGG                GTAATATTTAGA                AGTCCTCAGGACACCT CCACC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 005-143474515
Forward Primer: 5'-CCACTCTCCCTCAAATCAACTGC-3' Tm: 59.148
Reverse Primer: 5'-CTGCATTTCTGAGACAGACATGGC-3' Tm: 59.038
Expected PCR Product Length: 351
Expected CelI Cleavage Product Sizes: 202, 149
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr5:143474693-143474736

Rank: 141	Homology Score: 13	TALEN Score: 53.85
	Genomic Region: Exon	Closest Gene: CD300LD
Match Type: R16R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CCACA TGGTCCTGTCTCCTCT              CCTCTCCTTGGTGATC              ACAGGTGTCTGGTGCC CTTCA-3'
	3'-GGTGT ACCAGGACAGAGGAGA              GGAGAGGAACCACTAG              TGTCCACAGACCACGG GAAGT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 017-072588236
Forward Primer: 5'-CAGAGCGTCTCTGTCTCCAAG-3' Tm: 59.378
Reverse Primer: 5'-GGCACACCTTGCAACATACACTG-3' Tm: 59.148
Expected PCR Product Length: 329
Expected CelI Cleavage Product Sizes: 131, 198
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr17:72588341-72588388

Rank: 142	Homology Score: 10	TALEN Score: 53.85
	Genomic Region: Intergenic	Closest Gene: DCLK2
Match Type: R24R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GCAGG TTGACTTGACTCCTTG          TGGAATCACCACAGCATGACCTGG          ACAGGTGTCAGTGGCA ACAGC-3'
	3'-CGTCC AACTGAACTGAGGAAC          ACCTTAGTGGTGTCGTACTGGACC          TGTCCACAGTCACCGT TGTCG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 004-150284205
Forward Primer: 5'-GAGGAAGTCCCCAGTGAGAAAATG-3' Tm: 59.038
Reverse Primer: 5'-TATTCCTGGACCCACCCTACTTC-3' Tm: 59.148
Expected PCR Product Length: 309
Expected CelI Cleavage Product Sizes: 179, 130
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr4:150284354-150284409

Rank: 143	Homology Score: 8	TALEN Score: 53.85
	Genomic Region: Intron	Closest Gene: ATP2B2
Match Type: R19R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GGACA TGCAGCTGACACTGGT            ACTTTCTCCTCTTCTGGAC             AAGGGAGTGAGGTGTA GCCAG-3'
	3'-CCTGT ACGTCGACTGTGACCA            TGAAAGAGGAGAAGACCTG             TTCCCTCACTCCACAT CGGTC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 003-010453381
Forward Primer: 5'-TGTTGCAGACACTGGAACAGAGC-3' Tm: 59.148
Reverse Primer: 5'-CGTGAGAGTGGACTTGCAAGTCTG-3' Tm: 60.908
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 135, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr3:10453489-10453539

Rank: 144	Homology Score: 10	TALEN Score: 53.83
	Genomic Region: Intron	Closest Gene: PCDH9
Match Type: R17R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-ATGCT TGCACCACAGTTCTTT             GTGAATGGGGGATGTGG              ACTGGATTCAGGTGAA AGAAA-3'
	3'-TACGA ACGTGGTGTCAAGAAA             CACTTACCCCCTACACC              TGACCTAAGTCCACTT TCTTT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 013-067512369
Forward Primer: 5'-GGCATCAACAGGGCTTCTTAAACG-3' Tm: 59.038
Reverse Primer: 5'-CAATGTCAGTCGTTTCTGCATGAAGATAGG-3' Tm: 60.058
Expected PCR Product Length: 388
Expected CelI Cleavage Product Sizes: 227, 161
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr13:67512570-67512618

Rank: 145	Homology Score: 10	TALEN Score: 53.83
	Genomic Region: Intergenic	Closest Gene: CPXCR1
Match Type: R16R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TGATG TGTGCATGACTCTTGG              CCCAATGTTGAGTAAC              ACAGGAGTTAATTGCA TGGAC-3'
	3'-ACTAC ACACGTACTGAGAACC              GGGTTACAACTCATTG              TGTCCTCAATTAACGT ACCTG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Warning! Primer pair was generated using suboptimal parameters. 
Primer Index: 00X-088338909
Forward Primer: 5'-CCCCCCCAGAAAAGTGGTTTATG-3' Tm: 59.148
Reverse Primer: 5'-CAGATTTGGCTCACAGTAGCATACTTTACTC-3' Tm: 59.958
Expected PCR Product Length: 415
Expected CelI Cleavage Product Sizes: 197, 218
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chrX:88339080-88339127

Rank: 146	Homology Score: 10	TALEN Score: 53.83
	Genomic Region: Intron	Closest Gene: CDH4
Match Type: R13R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TGCAT TTCTCCTGACTTGTAT               TACTCATTTTACG                GCTGGAGACAGGTGCA CATGG-3'
	3'-ACGTA AAGAGGACTGAACATA               ATGAGTAAAATGC                CGACCTCTGTCCACGT GTACC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 020-059992627
Forward Primer: 5'-TGGCCCTGTTTCTGGCTGGA-3' Tm: 59.508
Reverse Primer: 5'-AGTACACTGAAGTGCAGTCTCTGG-3' Tm: 59.038
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 136, 134
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr20:59992739-59992783

Rank: 147	Homology Score: 8	TALEN Score: 53.81
	Genomic Region: Intron	Closest Gene: ASAP2
Match Type: R17R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CAGCT TCCACCTGGCCCCTGA             GAATTAATGAGGTCTGG              CCAGGCGGCAGCTGCA GTGCA-3'
	3'-GTCGA AGGTGGACCGGGGACT             CTTAATTACTCCAGACC              GGTCCGCCGTCGACGT CACGT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 002-009410094
Forward Primer: 5'-CCTCGGTAACTTGAGAGGAACCTA-3' Tm: 59.038
Reverse Primer: 5'-AAAACTCCACTCCAGCTCAGAGCG-3' Tm: 60.908
Expected PCR Product Length: 335
Expected CelI Cleavage Product Sizes: 196, 139
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr2:9410264-9410312

Rank: 148	Homology Score: 8	TALEN Score: 53.81
	Genomic Region: Intron	Closest Gene: KIAA0317
Match Type: R19R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TTCTC TACTCCTGACACCTTT            GTCTAGACAAAGATCTAGT             ACAGGAATCATTTGCT TCAGG-3'
	3'-AAGAG ATGAGGACTGTGGAAA            CAGATCTGTTTCTAGATCA             TGTCCTTAGTAAACGA AGTCC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 014-075136937
Forward Primer: 5'-GAGGATCAGAGCCCATCCAAATGT-3' Tm: 59.038
Reverse Primer: 5'-GGAACCTGACAACCTGTGTCCT-3' Tm: 59.258
Expected PCR Product Length: 443
Expected CelI Cleavage Product Sizes: 212, 231
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr14:75137122-75137172

Rank: 149	Homology Score: 10	TALEN Score: 53.76
	Genomic Region: Intron	Closest Gene: SLC16A2
Match Type: R21R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GCATG TTTACCTGATCCCTGA           TGTTCCAATCTCATAGTGACC            ACAGGAGCCTGGAGCA TAGTA-3'
	3'-CGTAC AAATGGACTAGGGACT           ACAAGGTTAGAGTATCACTGG            TGTCCTCGGACCTCGT ATCAT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 00X-073723246
Forward Primer: 5'-GTACAGTGGGGAGTCTGGAG-3' Tm: 59.508
Reverse Primer: 5'-CAGAAGACCAGAAGTAGGCCTAAC-3' Tm: 59.038
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 135, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chrX:73723353-73723405

Rank: 150	Homology Score: 10	TALEN Score: 53.75
	Genomic Region: Intron	Closest Gene: MED27
Match Type: R27R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TTGTT TACAAATCACTCCTGC        CATCAACCTCATTTTACAGATCAGGAA         ACAGGAGTGAGCTGAA CAACG-3'
	3'-AACAA ATGTTTAGTGAGGACG        GTAGTTGGAGTAAAATGTCTAGTCCTT         TGTCCTCACTCGACTT GTTGC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 009-134936820
Forward Primer: 5'-GCTGGTAAGTGAAGTTGATGTGGC-3' Tm: 59.038
Reverse Primer: 5'-CCAGGAGCAGCTGCGATGAA-3' Tm: 59.508
Expected PCR Product Length: 318
Expected CelI Cleavage Product Sizes: 187, 131
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr9:134936976-134937034

Rank: 151	Homology Score: 8	TALEN Score: 53.74
	Genomic Region: Intron	Closest Gene: APOL2
Match Type: R25R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-AGGCC TTCACCACACTCCTAT         GACATTGAATTGCTATTCCTATTGG          ACAGAAGACAGTTCCA AGTCT-3'
	3'-TCCGG AAGTGGTGTGAGGATA         CTGTAACTTAACGATAAGGATAACC          TGTCTTCTGTCAAGGT TCAGA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 022-036626609
Forward Primer: 5'-CTGACTCCAGGCTCTGACAC-3' Tm: 59.508
Reverse Primer: 5'-GCTGGGGCCTTTTTTCTCATCTG-3' Tm: 59.148
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 130, 140
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr22:36626709-36626765

Rank: 152	Homology Score: 10	TALEN Score: 53.67
	Genomic Region: Intron	Closest Gene: MLXIPL
Match Type: R17R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CACCC TTCACCCCATCCCTGT             CTGCTCAGCGCAAGGCT              ACAGGAGGCAGGGGCT GGTCT-3'
	3'-GTGGG AAGTGGGGTAGGGACA             GACGAGTCGCGTTCCGA              TGTCCTCCGTCCCCGA CCAGA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 007-073021712
Forward Primer: 5'-GATGCGCCGCTTCCAGTAGT-3' Tm: 59.508
Reverse Primer: 5'-TGTGAAGCGGAGGAAGAGCC-3' Tm: 59.508
Expected PCR Product Length: 290
Expected CelI Cleavage Product Sizes: 130, 160
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr7:73021816-73021864

Rank: 153	Homology Score: 5	TALEN Score: 53.66
	Genomic Region: Intergenic	Closest Gene: CNR2
Match Type: R19R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CAGCC TACATCAGACTCCTAT            CTGGGCTGGAATTGGAGAC             CCAGGGGTGAGCTGGA AGAGA-3'
	3'-GTCGG ATGTAGTCTGAGGATA            GACCCGACCTTAACCTCTG             GGTCCCCACTCGACCT TCTCT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 001-024242432
Forward Primer: 5'-TCCCAAGAGCAAGACATTGGCAG-3' Tm: 59.148
Reverse Primer: 5'-GCAATGGAGGACCAGGCGTT-3' Tm: 59.508
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 135, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr1:24242540-24242590

Rank: 154	Homology Score: 8	TALEN Score: 53.65
	Genomic Region: Intergenic	Closest Gene: N/A
Match Type: R20R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CTCAA TGAACTTTACTCCTCT            GAGGAAGGTTAGCAAGAGGG            ATAGGAGTCAGGAGAG GCACC-3'
	3'-GAGTT ACTTGAAATGAGGAGA            CTCCTTCCAATCGTTCTCCC            TATCCTCAGTCCTCTC CGTGG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 215-000126245
Forward Primer: 5'-GTCCCCTCTCCTTGCACTTTTC-3' Tm: 59.258
Reverse Primer: 5'-CCTTGGGAACCTTGGACAATAAGC-3' Tm: 59.038
Expected PCR Product Length: 341
Expected CelI Cleavage Product Sizes: 169, 172
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chrUn_gl000215:126386-126437

Rank: 155	Homology Score: 8	TALEN Score: 53.65
	Genomic Region: Intergenic	Closest Gene: C21orf15
Match Type: R20R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CTCAA TGAACTTTACTCCTCT            GAGGAAGGTTAGCAAGAGGG            ATAGGAGTCAGGAGAG GCACC-3'
	3'-GAGTT ACTTGAAATGAGGAGA            CTCCTTCCAATCGTTCTCCC            TATCCTCAGTCCTCTC CGTGG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 021-015154271
Forward Primer: 5'-GTCCCCTCTCCTTGCACTTTTC-3' Tm: 59.258
Reverse Primer: 5'-CCTTGGGAACCTTGGACAATAAGC-3' Tm: 59.038
Expected PCR Product Length: 341
Expected CelI Cleavage Product Sizes: 169, 172
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr21:15154412-15154463

Rank: 156	Homology Score: 5	TALEN Score: 53.65
	Genomic Region: Intergenic	Closest Gene: FAM65B
Match Type: R15R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CAGTT GGCCCCTCACACCTAT              ATGGTAAAGTGTGAA               TGAGGAGTCATGTGTA CACAG-3'
	3'-GTCAA CCGGGGAGTGTGGATA              TACCATTTCACACTT               ACTCCTCAGTACACAT GTGTC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 006-024980600
Forward Primer: 5'-GGGAGTGAAATCAAGACACCTGG-3' Tm: 59.148
Reverse Primer: 5'-AATGCCCAAAAGCCCCCTGTG-3' Tm: 59.378
Expected PCR Product Length: 369
Expected CelI Cleavage Product Sizes: 238, 131
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr6:24980813-24980859

Rank: 157	Homology Score: 5	TALEN Score: 53.65
	Genomic Region: Intergenic	Closest Gene: KIF4B
Match Type: R25R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CTAAA TGCACCTGACAACAAT         AATCTAAGCATACCCTTAGAGTCAC          CCTGTATTCAGATGCA CCTGA-3'
	3'-GATTT ACGTGGACTGTTGTTA         TTAGATTCGTATGGGAATCTCAGTG          GGACATAAGTCTACGT GGACT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 005-154638956
Forward Primer: 5'-GGGCAACAAGAGTGAGACTCTG-3' Tm: 59.258
Reverse Primer: 5'-CGTGTTCCACAAGATGGGCTG-3' Tm: 59.378
Expected PCR Product Length: 304
Expected CelI Cleavage Product Sizes: 172, 132
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr5:154639098-154639154

Rank: 158	Homology Score: 10	TALEN Score: 53.63
	Genomic Region: Intron	Closest Gene: PIBF1
Match Type: R23R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-AAATT TACCCCTCTCTCCTCT          GAATTTAGAACTATTAAGGAGAA           ACAAGAGTCAGTTACA TATCT-3'
	3'-TTTAA ATGGGGAGAGAGGAGA          CTTAAATCTTGATAATTCCTCTT           TGTTCTCAGTCAATGT ATAGA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 013-073489472
Forward Primer: 5'-GCGCTCTGCTTCTAAGACACATTG-3' Tm: 59.038
Reverse Primer: 5'-CACATGGAAGAGATGATACATGCCATTACC-3' Tm: 60.058
Expected PCR Product Length: 386
Expected CelI Cleavage Product Sizes: 243, 143
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr13:73489686-73489740

Rank: 159	Homology Score: 10	TALEN Score: 53.62
	Genomic Region: Intergenic	Closest Gene: KCNJ2
Match Type: R26R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GCCAC CGCACCTGGCTCCTCT         GGCTATTTTTGCCAGGATTTTAAACT         AAAAGTGTCAGGAGAA TGCTT-3'
	3'-CGGTG GCGTGGACCGAGGAGA         CCGATAAAAACGGTCCTAAAATTTGA         TTTTCACAGTCCTCTT ACGAA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 017-068378451
Forward Primer: 5'-TGGGCTTCCTCCATGTTGGTC-3' Tm: 59.378
Reverse Primer: 5'-ATGACAGGACCTGGCTCACC-3' Tm: 59.508
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 132, 138
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr17:68378552-68378609

Rank: 160	Homology Score: 13	TALEN Score: 53.62
	Genomic Region: Intron	Closest Gene: SCRN1
Match Type: R10R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GTCAC TGTACCTGACTGATGT                 ATACTTTTTA                 TCATAAGCCAGGTGCA GTGGC-3'
	3'-CAGTG ACATGGACTGACTACA                 TATGAAAAAT                 AGTATTCGGTCCACGT CACCG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 007-030006854
Forward Primer: 5'-CCAAGAAGGTTATGCCCTGAATGC-3' Tm: 59.038
Reverse Primer: 5'-TAGTGGGTGTCCTTGGCTCTG-3' Tm: 59.378
Expected PCR Product Length: 433
Expected CelI Cleavage Product Sizes: 303, 130
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr7:30007134-30007175

Rank: 161	Homology Score: 10	TALEN Score: 53.62
	Genomic Region: Intergenic	Closest Gene: GHITM
Match Type: R29R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TAAGC TGCATCTTGCTCCTGT       ATGTGGACAAACTTCTGCTGGGACACTCC        ACGGCAGTCGGGTGTG CAAAA-3'
	3'-ATTCG ACGTAGAACGAGGACA       TACACCTGTTTGAAGACGACCCTGTGAGG        TGCCGTCAGCCCACAC GTTTT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 010-085871707
Forward Primer: 5'-AGTACACTTGGACCCGGCTTC-3' Tm: 59.378
Reverse Primer: 5'-TGAATCCCAGGTAGCGTACCTGC-3' Tm: 61.088
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 136, 134
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr10:85871811-85871871

Rank: 162	Homology Score: 8	TALEN Score: 53.61
	Genomic Region: Intergenic	Closest Gene: OGFRL1
Match Type: R12R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CCTCC TGCCTCTGCCTCCTGA                GTAGCTGGGATT                ATAGGAGTGAGCTGCT GTGCC-3'
	3'-GGAGG ACGGAGACGGAGGACT                CATCGACCCTAA                TATCCTCACTCGACGA CACGG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 006-071979511
Forward Primer: 5'-GACATTCTAGAGCAGTTTGGCAGG-3' Tm: 59.038
Reverse Primer: 5'-GTGACTGTGGGTGAAGATGCTG-3' Tm: 59.258
Expected PCR Product Length: 334
Expected CelI Cleavage Product Sizes: 174, 160
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr6:71979661-71979704

Rank: 163	Homology Score: 13	TALEN Score: 53.61
	Genomic Region: Intron	Closest Gene: HPSE2
Match Type: R14R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CAGGC TCCACATGCCTCCTGT               GTGAAATCCCCCCA               ACAAGACTCAGATGCC TCAGG-3'
	3'-GTCCG AGGTGTACGGAGGACA               CACTTTAGGGGGGT               TGTTCTGAGTCTACGG AGTCC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 010-100332368
Forward Primer: 5'-GAGAGTGAACTCACCTTCCATGAC-3' Tm: 59.038
Reverse Primer: 5'-TGGGCACACAGTCAGTTCCTAG-3' Tm: 59.258
Expected PCR Product Length: 304
Expected CelI Cleavage Product Sizes: 174, 130
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr10:100332517-100332562

Rank: 164	Homology Score: 10	TALEN Score: 53.59
	Genomic Region: Intergenic	Closest Gene: KCNJ2
Match Type: R21R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TATGC TACATGTGACTCCTAA           TTAAGTGATCTTTCCAGAACA            CCAAGAGTCAAGTGCA GTCGG-3'
	3'-ATACG ATGTACACTGAGGATT           AATTCACTAGAAAGGTCTTGT            GGTTCTCAGTTCACGT CAGCC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Warning! Primer pair was generated using suboptimal parameters. 
Primer Index: 017-068268613
Forward Primer: 5'-AGGAAGGGATTCTCCTAATAAATTGGCAGATG-3' Tm: 59.848
Reverse Primer: 5'-CTATGGTCCATGTCTAACGTTAATACTACATGC-3' Tm: 59.758
Expected PCR Product Length: 391
Expected CelI Cleavage Product Sizes: 130, 261
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr17:68268715-68268767

Rank: 165	Homology Score: 5	TALEN Score: 53.55
	Genomic Region: Intergenic	Closest Gene: MIR548AE2
Match Type: R25L
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TGCCA TGTACCAGACACCTCT         GGAGATATTACACTGAGTTTCCACA    ACCACCCTGTGGGGCAAGCTTT AGGAG-3'
	3'-ACGGT ACATGGTCTGTGGAGA         CCTCTATAATGTGACTCAAAGGTGT    TGGTGGGACACCCCGTTCGAAA TCCTC-5'
	                                                               TGACGGGACACCCCGTTCCACT
	                                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 016-048484957
Forward Primer: 5'-CCAGGGAAGCATCATGCCCTTT-3' Tm: 59.258
Reverse Primer: 5'-CAGCCTGCAGAATGGAAGGG-3' Tm: 59.508
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 136, 134
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr16:48485060-48485122

Rank: 166	Homology Score: 8	TALEN Score: 53.54
	Genomic Region: Intergenic	Closest Gene: ZIC3
Match Type: R25R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-AAGAT ATCACCTCACACCTGT         TAGAAGAGCTTCTGTCAAAAAGACA          AAAGAGGCCAGGTGCA GTGGC-3'
	3'-TTCTA TAGTGGAGTGTGGACA         ATCTTCTCGAAGACAGTTTTTCTGT          TTTCTCCGGTCCACGT CACCG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 00X-136731121
Forward Primer: 5'-GTCACTTTACCTCCCTGAACCTC-3' Tm: 59.148
Reverse Primer: 5'-AGACAGCATTTCGCCATGTTGACC-3' Tm: 59.038
Expected PCR Product Length: 442
Expected CelI Cleavage Product Sizes: 311, 131
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chrX:136731402-136731458

Rank: 167	Homology Score: 10	TALEN Score: 53.52
	Genomic Region: Intergenic	Closest Gene: LOC100507173
Match Type: R12R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GGCCT GGCAACTGACACCTGT                AATCCCAGCTAC                TCGGGAGGCAGAGGCA GAGGC-3'
	3'-CCGGA CCGTTGACTGTGGACA                TTAGGGTCGATG                AGCCCTCCGTCTCCGT CTCCG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 006-027625538
Forward Primer: 5'-ATCCGAGAACTGTGGGAGGC-3' Tm: 59.508
Reverse Primer: 5'-CTCCCTGTTAATGCCACCCC-3' Tm: 59.508
Expected PCR Product Length: 334
Expected CelI Cleavage Product Sizes: 142, 192
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr6:27625656-27625699

Rank: 168	Homology Score: 10	TALEN Score: 53.49
	Genomic Region: Intron	Closest Gene: DSCAML1
Match Type: R23R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-ACGGT GCCATCTGACACCAGT          CCGTGGGTGAGGAGGCGTCTGGG           ACAGGAGTAGGGTGGA TGAAG-3'
	3'-TGCCA CGGTAGACTGTGGTCA          GGCACCCACTCCTCCGCAGACCC           TGTCCTCATCCCACCT ACTTC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 011-117354866
Forward Primer: 5'-GCTCAGGCAAAGTTGTTCCCAG-3' Tm: 59.258
Reverse Primer: 5'-ATGTGCTGAGAGGCTGGTGC-3' Tm: 59.508
Expected PCR Product Length: 286
Expected CelI Cleavage Product Sizes: 130, 156
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr11:117354967-117355021

Rank: 169	Homology Score: 8	TALEN Score: 53.48
	Genomic Region: Intergenic	Closest Gene: ALAS1
Match Type: R15R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TGAGC TGCAGTTGCCTCATGT              GAAATGAAGATTGTA               ACTGAAGCCAGGTGTA TCATT-3'
	3'-ACTCG ACGTCAACGGAGTACA              CTTTACTTCTAACAT               TGACTTCGGTCCACAT AGTAA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 003-052213069
Forward Primer: 5'-CATAGGGGTGAGCTGAGCTCAT-3' Tm: 59.258
Reverse Primer: 5'-GGCCTTTGGAAGGAGAATGGCT-3' Tm: 59.258
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 135, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr3:52213179-52213225

Rank: 170	Homology Score: 2	TALEN Score: 53.45
	Genomic Region: Intergenic	Closest Gene: BARX1
Match Type: R16L
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-AGGGA TGCCCCTGGCACCAGC              CTCAGCAGGCCGGGCA        CCTGCACTGTGAGGGCAGGTGA GCCAA-3'
	3'-TCCCT ACGGGGACCGTGGTCG              GAGTCGTCCGGCCCGT        GGACGTGACACTCCCGTCCACT CGGTT-5'
	                                                               TGACGGGACACCCCGTTCCACT
	                                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 009-096658949
Forward Primer: 5'-AGAGAGAGGCCCTGGAAGGT-3' Tm: 59.508
Reverse Primer: 5'-TTCCTAGGCCTTGGCCCTCT-3' Tm: 59.508
Expected PCR Product Length: 333
Expected CelI Cleavage Product Sizes: 197, 136
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr9:96659117-96659170

Rank: 171	Homology Score: 5	TALEN Score: 53.45
	Genomic Region: Intergenic	Closest Gene: PLD5
Match Type: R18R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TCCAT AACACCGGGCTCCTGG             TAGCTCTGCCCATAAACT             AAAGGATTCAGGTTTA ATTCA-3'
	3'-AGGTA TTGTGGCCCGAGGACC             ATCGAGACGGGTATTTGA             TTTCCTAAGTCCAAAT TAAGT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 001-242741404
Forward Primer: 5'-CTTGAAGGCTCCAACTTCATGTCC-3' Tm: 59.038
Reverse Primer: 5'-CATTCTGGGCTCTTCATAAGGCTC-3' Tm: 59.038
Expected PCR Product Length: 378
Expected CelI Cleavage Product Sizes: 147, 231
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr1:242741524-242741573

Rank: 172	Homology Score: 10	TALEN Score: 53.44
	Genomic Region: Intron	Closest Gene: TMED3
Match Type: R17R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TCCTG TATTCCTGTCTCCTTT             GAGAGATCCCGTGGCCC              ACAGGTGACAGGAGCA GGTCT-3'
	3'-AGGAC ATAAGGACAGAGGAAA             CTCTCTAGGGCACCGGG              TGTCCACTGTCCTCGT CCAGA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 015-079604259
Forward Primer: 5'-TTGGAAAGGAGGGATCAGTGCAG-3' Tm: 59.148
Reverse Primer: 5'-GTGCCTATCAGCATGCGTCC-3' Tm: 59.508
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 135, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr15:79604368-79604416

Rank: 173	Homology Score: 5	TALEN Score: 53.43
	Genomic Region: Intergenic	Closest Gene: PKIA
Match Type: R24R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TAGAA AACATCTGAAACCTGT          GGTTACCAGCTTCCAGATCAGATC          TCAGGAGTCTGGCGAA GTGGG-3'
	3'-ATCTT TTGTAGACTTTGGACA          CCAATGGTCGAAGGTCTAGTCTAG          AGTCCTCAGACCGCTT CACCC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 008-078830967
Forward Primer: 5'-CAGGAATGTCAGGTGACTCTCAG-3' Tm: 59.148
Reverse Primer: 5'-CACTGGAACTATGCACATGCTCAC-3' Tm: 59.038
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 139, 131
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr8:78831076-78831131

Rank: 174	Homology Score: 5	TALEN Score: 53.42
	Genomic Region: Intergenic	Closest Gene: LSAMP-AS3
Match Type: R12R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-AGTTT TGCACCAAAATACTGT                GAAAGAGAAGAA                AGTGGTGGCAGGTGCT GGTGG-3'
	3'-TCAAA ACGTGGTTTTATGACA                CTTTCTCTTCTT                TCACCACCGTCCACGA CCACC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 003-116802161
Forward Primer: 5'-GGGATGGTCCAAAACTCAAACCTC-3' Tm: 59.038
Reverse Primer: 5'-GAAGGCATTGGGGCTAGACAAG-3' Tm: 59.258
Expected PCR Product Length: 362
Expected CelI Cleavage Product Sizes: 177, 185
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr3:116802314-116802357

Rank: 175	Homology Score: 8	TALEN Score: 53.4
	Genomic Region: Intron	Closest Gene: CAMK2G
Match Type: R11R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CCCAG CCCACCTGTCTCCTGC                TGACCTGGAAA                 GCAGGAGGCAGGCTCA GGAGC-3'
	3'-GGGTC GGGTGGACAGAGGACG                ACTGGACCTTT                 CGTCCTCCGTCCGAGT CCTCG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 010-075583632
Forward Primer: 5'-GGTATAATGCCCTTCCTCAGCATG-3' Tm: 59.038
Reverse Primer: 5'-CAGGGAAGAGCCATAGTCTGTC-3' Tm: 59.258
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 134, 136
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr10:75583743-75583785

Rank: 176	Homology Score: 5	TALEN Score: 53.4
	Genomic Region: Intron	Closest Gene: C1orf21
Match Type: R18R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-ATTCA TCCACCTCCCTCCTAT             GTGGTGTAATAAACATGT             TCAGGAGTTAGATTCT TGTGA-3'
	3'-TAAGT AGGTGGAGGGAGGATA             CACCACATTATTTGTACA             AGTCCTCAATCTAAGA ACACT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 001-184575055
Forward Primer: 5'-GGTGGTGCTAAACCGAACCTG-3' Tm: 59.378
Reverse Primer: 5'-GCCACTACACCTGGCCAATG-3' Tm: 59.508
Expected PCR Product Length: 389
Expected CelI Cleavage Product Sizes: 157, 232
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr1:184575185-184575234

Rank: 177	Homology Score: 5	TALEN Score: 53.39
	Genomic Region: Intergenic	Closest Gene: PRELID2
Match Type: R19R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GACAG AGCCCCTCAATCCTGT            GATTGTAGCATGTTCTTTT             ATTTGAGTAAGGTGTA CAGTT-3'
	3'-CTGTC TCGGGGAGTTAGGACA            CTAACATCGTACAAGAAAA             TAAACTCATTCCACAT GTCAA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 005-144614657
Forward Primer: 5'-CAGTGACCTTAGCAGACTATCAGC-3' Tm: 59.038
Reverse Primer: 5'-CTCTGGGCTTGACAGCAACTGA-3' Tm: 59.258
Expected PCR Product Length: 340
Expected CelI Cleavage Product Sizes: 209, 131
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr5:144614839-144614889

Rank: 178	Homology Score: 8	TALEN Score: 53.39
	Genomic Region: Intron	Closest Gene: NPC1L1
Match Type: R21R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CCCTC TTCACCTCAGCCCTGT           GACATAGCCCCCAGGGCTTCT            GCAGGTGGCAGGTTCA TTCTG-3'
	3'-GGGAG AAGTGGAGTCGGGACA           CTGTATCGGGGGTCCCGAAGA            CGTCCACCGTCCAAGT AAGAC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 007-044570049
Forward Primer: 5'-GGAGATGGATGGTGGTGATAGTTG-3' Tm: 59.038
Reverse Primer: 5'-TTAGGCAGTACTGGCCTGGG-3' Tm: 59.508
Expected PCR Product Length: 434
Expected CelI Cleavage Product Sizes: 294, 140
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr7:44570315-44570367

Rank: 179	Homology Score: 10	TALEN Score: 53.39
	Genomic Region: Exon	Closest Gene: TMEM132C
Match Type: R27R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TCGTG ATCACCTGACTCCAGT        TCTCCGTGTGCTCCATTGGCTGCGGCT         GCAGGAGGAAGATGCC TGACA-3'
	3'-AGCAC TAGTGGACTGAGGTCA        AGAGGCACACGAGGTAACCGACGCCGA         CGTCCTCCTTCTACGG ACTGT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 012-129191997
Forward Primer: 5'-GGTGGGGTATACCAGCATAAACTC-3' Tm: 59.038
Reverse Primer: 5'-GGCTTCAAGGACCCTTTCTTAGCT-3' Tm: 59.038
Expected PCR Product Length: 337
Expected CelI Cleavage Product Sizes: 197, 140
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr12:129192163-129192221

Rank: 180	Homology Score: 8	TALEN Score: 53.36
	Genomic Region: Intron	Closest Gene: HTR3D
Match Type: R17R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TTCTC TGCTCCTGCCTCCTTC             CCTGTCTCCCTCCCTCC              ACAGGTGACATTTGCA GCCCA-3'
	3'-AAGAG ACGAGGACGGAGGAAG             GGACAGAGGGAGGGAGG              TGTCCACTGTAAACGT CGGGT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 003-183756342
Forward Primer: 5'-AGGGAGATGCTGTCCCACTG-3' Tm: 59.508
Reverse Primer: 5'-TCCATTCTGAGCCCCCTGTC-3' Tm: 59.508
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 139, 131
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr3:183756455-183756503

Rank: 181	Homology Score: 8	TALEN Score: 53.36
	Genomic Region: Intron	Closest Gene: HTR3E
Match Type: R17R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TTCTC TGCTCCTGCCTCCTTC             CCTGTCTCCCTCCCTCC              ACAGGTGACATTTGCA GCCCA-3'
	3'-AAGAG ACGAGGACGGAGGAAG             GGACAGAGGGAGGGAGG              TGTCCACTGTAAACGT CGGGT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 003-183824037
Forward Primer: 5'-CCTGCTGCTCCACTGCAACA-3' Tm: 59.508
Reverse Primer: 5'-TCCATTCTGAGCCCCCTGTC-3' Tm: 59.508
Expected PCR Product Length: 293
Expected CelI Cleavage Product Sizes: 162, 131
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr3:183824173-183824221

Rank: 182	Homology Score: 10	TALEN Score: 53.36
	Genomic Region: Intergenic	Closest Gene: SLC16A7
Match Type: R15R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TCTCT CACATCTGCCTCCAGT              GTACCAGCAAAGATC               ACAGGGGTCAGGTCAA GGTGG-3'
	3'-AGAGA GTGTAGACGGAGGTCA              CATGGTCGTTTCTAG               TGTCCCCAGTCCAGTT CCACC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Warning! Primer pair was generated using suboptimal parameters. 
Primer Index: 012-059855477
Forward Primer: 5'-GAGAAAGGATTGAGTATTTTTCTCAGGACTTGG-3' Tm: 59.758
Reverse Primer: 5'-CAGAATTTCAGAGGCCCTCCATC-3' Tm: 59.148
Expected PCR Product Length: 404
Expected CelI Cleavage Product Sizes: 130, 274
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr12:59855582-59855628

Rank: 183	Homology Score: 10	TALEN Score: 53.34
	Genomic Region: Intron	Closest Gene: PFKFB3
Match Type: R20R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TCCTG TGCACCTGTCTCATTT            CCTCAACAAGGAATAGAATG            CTTGCAGTCAGGTGCC CCAGC-3'
	3'-AGGAC ACGTGGACAGAGTAAA            GGAGTTGTTCCTTATCTTAC            GAACGTCAGTCCACGG GGTCG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 010-006225239
Forward Primer: 5'-CGCCAACTTTGGGGTTGGCT-3' Tm: 59.508
Reverse Primer: 5'-AAGTGCTGGGATTACAGGCATGAG-3' Tm: 59.038
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 138, 132
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr10:6225349-6225400

Rank: 184	Homology Score: 10	TALEN Score: 53.34
	Genomic Region: Intergenic	Closest Gene: GOSR2
Match Type: R16R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GAGGC TGCAACTGACTGCTGC              CCCTGGGGAAGGTGGG              ACGGGGGCCAGATTCA CAGGG-3'
	3'-CTCCG ACGTTGACTGACGACG              GGGACCCCTTCCACCC              TGCCCCCGGTCTAAGT GTCCC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 017-045024202
Forward Primer: 5'-CTGGGTGACGAAATGAGACTCTG-3' Tm: 59.148
Reverse Primer: 5'-TTCATGTCAGGGTCCCCTGG-3' Tm: 59.508
Expected PCR Product Length: 308
Expected CelI Cleavage Product Sizes: 161, 147
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr17:45024337-45024384

Rank: 185	Homology Score: 5	TALEN Score: 53.34
	Genomic Region: Intergenic	Closest Gene: FLRT2
Match Type: R26R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-AGTGT TGTCCCTGACTCCGAT         TCTCATTGTCCTATTCTCAATCGGGA         AATGGAGGCTGGTGGA ACCAA-3'
	3'-TCACA ACAGGGACTGAGGCTA         AGAGTAACAGGATAAGAGTTAGCCCT         TTACCTCCGACCACCT TGGTT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 014-086334283
Forward Primer: 5'-CACACACACACACACCCTTTGATC-3' Tm: 59.038
Reverse Primer: 5'-CAGCAACGCTGACCTAGAAACTC-3' Tm: 59.148
Expected PCR Product Length: 419
Expected CelI Cleavage Product Sizes: 255, 164
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr14:86334507-86334564

Rank: 186	Homology Score: 5	TALEN Score: 53.32
	Genomic Region: Intron	Closest Gene: VWF
Match Type: R26R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TCGGG TACTGCTGACTCCAGG         TTGCTGACATGGGGGTTTCCTTTCAC         ACAGGGGTTAGTGGCA TTCAG-3'
	3'-AGCCC ATGACGACTGAGGTCC         AACGACTGTACCCCCAAAGGAAAGTG         TGTCCCCAATCACCGT AAGTC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 012-006059468
Forward Primer: 5'-GGTAAGAGGAAAGGCGAAGTCATG-3' Tm: 59.038
Reverse Primer: 5'-CAGGGCCTTAAATCCACAGCAAC-3' Tm: 59.148
Expected PCR Product Length: 421
Expected CelI Cleavage Product Sizes: 131, 290
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr12:6059568-6059625

Rank: 187	Homology Score: 13	TALEN Score: 53.32
	Genomic Region: Intergenic	Closest Gene: SLC25A47
Match Type: R28R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CCTGC TGCAGCTGGCTGTTGT        CCCCGTTCACAGATAGGGAAACCGAGGC        ACAGGAGTTAAGTCCA TTGGC-3'
	3'-GGACG ACGTCGACCGACAACA        GGGGCAAGTGTCTATCCCTTTGGCTCCG        TGTCCTCAATTCAGGT AACCG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 014-100782711
Forward Primer: 5'-TGAAGGGACAGAGCCAGGGT-3' Tm: 59.508
Reverse Primer: 5'-ACACCAGCCTGGGCAACAGA-3' Tm: 59.508
Expected PCR Product Length: 286
Expected CelI Cleavage Product Sizes: 156, 130
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr14:100782835-100782894

Rank: 188	Homology Score: 8	TALEN Score: 53.31
	Genomic Region: Intron	Closest Gene: C3orf55
Match Type: R29R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TTTTG CACACCAGACTGCTGT       GGAGTGTCCCAGCAGAAGGTCATCCACGT        ACAGGAGCAAGACGCA GCCTA-3'
	3'-AAAAC GTGTGGTCTGACGACA       CCTCACAGGGTCGTCTTCCAGTAGGTGCA        TGTCCTCGTTCTGCGT CGGAT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 003-157302016
Forward Primer: 5'-ATCCCAGGTAGCGTACCTGC-3' Tm: 59.508
Reverse Primer: 5'-CTTAGTACACTTGGACCTGGCTTC-3' Tm: 59.038
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 134, 136
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr3:157302118-157302178

Rank: 189	Homology Score: 10	TALEN Score: 53.31
	Genomic Region: Intron	Closest Gene: PLCG1
Match Type: R20R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-AGCTT CTCTCCAGACTCTTGT            GTGTGTGTGTACACAGATGC            ACAGGATTCAGTTGGA GTTTA-3'
	3'-TCGAA GAGAGGTCTGAGAACA            CACACACACATGTGTCTACG            TGTCCTAAGTCAACCT CAAAT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 020-039770399
Forward Primer: 5'-CCAGCTGGGATCCTCTGTTTC-3' Tm: 59.378
Reverse Primer: 5'-CCTAGCACTGGGCAAATGGG-3' Tm: 59.508
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 135, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr20:39770506-39770557

Rank: 190	Homology Score: 10	TALEN Score: 53.3
	Genomic Region: Exon	Closest Gene: KIAA1244
Match Type: R19R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CATTG TGTAGCCGACTCCTGT            TCTACTCTCCCACCAAATA             ACAGTAGTGAGGGTTA GAGTC-3'
	3'-GTAAC ACATCGGCTGAGGACA            AGATGAGAGGGTGGTTTAT             TGTCATCACTCCCAAT CTCAG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 006-138657515
Forward Primer: 5'-GTTCCCGTGCATCAGTCAGC-3' Tm: 59.508
Reverse Primer: 5'-GCTGATCAGTGGTTGGAAAGGC-3' Tm: 59.258
Expected PCR Product Length: 272
Expected CelI Cleavage Product Sizes: 131, 141
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr6:138657619-138657669

Rank: 191	Homology Score: 10	TALEN Score: 53.3
	Genomic Region: Intergenic	Closest Gene: SSBP3
Match Type: R10R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TCGTC TGTGCCTGACTCCTGC                 TCCAAGGAGC                 ACAGGTATCGGATGGA TATCC-3'
	3'-AGCAG ACACGGACTGAGGACG                 AGGTTCCTCG                 TGTCCATAGCCTACCT ATAGG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 001-054689628
Forward Primer: 5'-GTGAAGGCAACTGCCAGGCT-3' Tm: 59.508
Reverse Primer: 5'-GGCTTTTGCTGAGTACAGACACC-3' Tm: 59.148
Expected PCR Product Length: 313
Expected CelI Cleavage Product Sizes: 156, 157
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr1:54689761-54689802

Rank: 192	Homology Score: 5	TALEN Score: 53.29
	Genomic Region: Intron	Closest Gene: CNNM3
Match Type: R15R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GCCAC CGCACCTGGCCCCTGC              CTAGTTCTCTTGTGT               TGAGGTGTTAGGGGCA GCTGC-3'
	3'-CGGTG GCGTGGACCGGGGACG              GATCAAGAGAACACA               ACTCCACAATCCCCGT CGACG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 002-097493184
Forward Primer: 5'-TAGTAGAGGCGGGGTTTTGCCA-3' Tm: 59.258
Reverse Primer: 5'-AAAAAGGGACAGTGTCCACTGGG-3' Tm: 59.148
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 135, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr2:97493294-97493340

Rank: 193	Homology Score: 8	TALEN Score: 53.27
	Genomic Region: Intron	Closest Gene: USP34
Match Type: R30R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TTTCT TACACCTGATTACTGC       CAATGATGGGTTTTGGCCTTTTACCAAGTA       CCATGACCCAGGTGCA TAATC-3'
	3'-AAAGA ATGTGGACTAATGACG       GTTACTACCCAAAACCGGAAAATGGTTCAT       GGTACTGGGTCCACGT ATTAG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 002-061428882
Forward Primer: 5'-GGTTAAAGCTGTCCGAGTAGCTAG-3' Tm: 59.038
Reverse Primer: 5'-GCTGGTTCCTGGTAACCTGG-3' Tm: 59.508
Expected PCR Product Length: 326
Expected CelI Cleavage Product Sizes: 190, 136
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr2:61429039-61429100

Rank: 194	Homology Score: 10	TALEN Score: 53.27
	Genomic Region: Intron	Closest Gene: MBP
Match Type: R20R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GGCTC TGCTCCTCTCTCCTGT            CTTGGCTGGCAGGGGCTGCC            CCTGGGGTCGGGTGGA CTCCA-3'
	3'-CCGAG ACGAGGAGAGAGGACA            GAACCGACCGTCCCCGACGG            GGACCCCAGCCCACCT GAGGT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 018-074790610
Forward Primer: 5'-GAGCTGTGGACTGAAGAGCAAC-3' Tm: 59.258
Reverse Primer: 5'-CACAGTCTTGGGAGGGTGCT-3' Tm: 59.508
Expected PCR Product Length: 304
Expected CelI Cleavage Product Sizes: 133, 171
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr18:74790715-74790766

Rank: 195	Homology Score: 8	TALEN Score: 53.27
	Genomic Region: Intron	Closest Gene: FAM205A
Match Type: R19R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GGCAG AGCACCTGGCTCTTGG            CTCCAGAGGTCACAGAGGG             ACAGGATTCATGGGAA ACACC-3'
	3'-CCGTC TCGTGGACCGAGAACC            GAGGTCTCCAGTGTCTCCC             TGTCCTAAGTACCCTT TGTGG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 009-034728408
Forward Primer: 5'-TTCATTTCCAGGGCATTGAGGTACAGG-3' Tm: 60.438
Reverse Primer: 5'-GCAAAGGAAGTCCAGGAGAGTG-3' Tm: 59.258
Expected PCR Product Length: 387
Expected CelI Cleavage Product Sizes: 130, 257
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr9:34728511-34728561

Rank: 196	Homology Score: 8	TALEN Score: 53.26
	Genomic Region: Intron	Closest Gene: RPL18A
Match Type: R30R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TAGGG TGGCCCTGCCTCCTGC       TTCCCGAATCTGTGGTCACTAGATGTAGGA       AAGGGAGTGAGGTGGA TCTCC-3'
	3'-ATCCC ACCGGGACGGAGGACG       AAGGGCTTAGACACCAGTGATCTACATCCT       TTCCCTCACTCCACCT AGAGG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 019-017971876
Forward Primer: 5'-CTCAATTAATCCTCCCTGGAGACC-3' Tm: 59.038
Reverse Primer: 5'-ATTCGCATGCGGTAGAGGGG-3' Tm: 59.508
Expected PCR Product Length: 303
Expected CelI Cleavage Product Sizes: 143, 160
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr19:17971986-17972047

Rank: 197	Homology Score: 8	TALEN Score: 53.25
	Genomic Region: Intron	Closest Gene: ZFPM1
Match Type: R29R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-ACGCC TTCACCTGCCTCCTCA       AGTCAGAGCTGTGAGCCAGCCTGCCAGGG        CCAGGGCTCAGGTGGA GAAAC-3'
	3'-TGCGG AAGTGGACGGAGGAGT       TCAGTCTCGACACTCGGTCGGACGGTCCC        GGTCCCGAGTCCACCT CTTTG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 016-088575852
Forward Primer: 5'-CCATTAGGCACACAGGGAGG-3' Tm: 59.508
Reverse Primer: 5'-CTGCCTCCTGAACCCACTCT-3' Tm: 59.508
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 135, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr16:88575955-88576015

Rank: 198	Homology Score: 10	TALEN Score: 53.24
	Genomic Region: Intergenic	Closest Gene: LOC200772
Match Type: R29R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CCCAC TGCACCTGTCACCTCT       GGCCCAGCACAAGGGCTGCATGTGGCCAC        ACCTTAGGCAGGTCCA ACCGC-3'
	3'-GGGTG ACGTGGACAGTGGAGA       CCGGGTCGTGTTCCCGACGTACACCGGTG        TGGAATCCGTCCAGGT TGGCG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 002-241889867
Forward Primer: 5'-TCAGAGCCTGAAGCAGGAGC-3' Tm: 59.508
Reverse Primer: 5'-GGGCAAGTGGATGGGTGGAT-3' Tm: 59.508
Expected PCR Product Length: 287
Expected CelI Cleavage Product Sizes: 135, 152
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr2:241889970-241890030

Rank: 199	Homology Score: 10	TALEN Score: 53.22
	Genomic Region: Intron	Closest Gene: ACSL6
Match Type: R28R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TCCCA AATACCTGACTCCTGT        TCCAGGTCCTTCCCTGGGCCCTCGGCCC        ACAGAAGTCCTGGACA CTCAA-3'
	3'-AGGGT TTATGGACTGAGGACA        AGGTCCAGGAAGGGACCCGGGAGCCGGG        TGTCTTCAGGACCTGT GAGTT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 005-131322201
Forward Primer: 5'-CTGCATCTTCTGGAGAGTTTGCC-3' Tm: 59.148
Reverse Primer: 5'-TTGAGCGAGATGGCGTGCAG-3' Tm: 59.508
Expected PCR Product Length: 287
Expected CelI Cleavage Product Sizes: 145, 142
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr5:131322314-131322373

Rank: 200	Homology Score: 10	TALEN Score: 53.22
	Genomic Region: Intergenic	Closest Gene: BTG2
Match Type: R22R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TTCTG TAGGCCTGACTCCTGT           AAGGAGCCTTTGGTAAGGAAGG           TGAGGACTCAGGTGTC CTGGC-3'
	3'-AAGAC ATCCGGACTGAGGACA           TTCCTCGGAAACCATTCCTTCC           ACTCCTGAGTCCACAG GACCG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 001-203281134
Forward Primer: 5'-TGCATGCTGCAGATTAAGAGCCC-3' Tm: 59.148
Reverse Primer: 5'-TAAGAAGTACCAGGACCACAACCC-3' Tm: 59.038
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 137, 133
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr1:203281242-203281295

Plate format Oligo Order Form
Well
Name
Sequence
5' mods
Internal
Notes
Primer Plate #1
A1
011-005248069F
GTGCCTATCAGAAACCCAAGAGTC
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
A2
011-005248069R
CAATCTACTCCCAGGAGCAGG
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
B1
011-005255477F
GTCAGTGCCTATCAGAAACCCAAG
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
B2
011-005255477R
TCACTGGAGCAGGGAGGACA
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
C1
003-058640715F
CATATGAGGAAACTGAGAGGCGG
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
C2
003-058640715R
CCTCGAGGAGGGCTATTCTAGA
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
D1
006-110284189F
GCAATGTTGCTCCAAGAGGCC
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
D2
006-110284189R
GAACTTATTCCTCCCACCTAGCTG
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
E1
002-205984816F
GTGGGTACTGGCTGCTAAGTATG
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
E2
002-205984816R
GTTCCTAGCAGCCACAGCAC
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
F1
022-045287459F
CAAAGGCTCTGCCTGCAGGA
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
F2
022-045287459R
CAGGACTAAGGCCGCTCTTC
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
G1
020-003459687F
GCCAGTGTGCCCAGCCAATT
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
G2
020-003459687R
GTGTACGTAGCTGTCTTCCCTTC
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
H1
005-059619945F
CCAAGACTTAGCTTAGCACCCAAC
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
H2
005-059619945R
CATGCCTGGAGTTAGCCTCTCT
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
A3
014-077327606F
GCCTGGTATAGAACAAGCACCTG
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
A4
014-077327606R
CCTCCTGTGATTGCCAGAGC
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
B3
007-002962408F
ACAGCGTTTGTCTCCTGGCC
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
B4
007-002962408R
GCCCCTAAGCCTTGCATTCAG
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
C3
015-082621505F
TGGCTTTCACGGGTCTGTGC
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
C4
015-082621505R
TAGACATGCAGCCTGGAGCC
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
D3
015-082918832F
TGGCTTTCACGGGTCTGTGC
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
D4
015-082918832R
TAGACATGCAGCCTGGAGCC
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
E3
015-085803797F
TAGACATGCAGCCTGGAGCC
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
E4
015-085803797R
TGGCTTTCACGGGTCTGTGC
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
F3
00X-034076224F
AGTATTGCCCTTTCTGCTAGGGAC
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
F4
00X-034076224R
CACTGCACACTTCTGTGGCTC
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
G3
006-123066634F
GTAGTGGGATCGTGCCTGTG
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
G4
006-123066634R
TCAGTACACTTGGACCCGGC
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
H3
dom-000216374F
TCTCCTTTCTGTACCTCAGAGACC
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
H4
dom-000216374R
CCCACCCTGGTACAGCAAATG
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
A5
002-199208411F
GCCTTACACATAACCTCACTGCC
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
A6
002-199208411R
CTCCAACATAACTCAGCCTCGAAG
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
B5
003-054731501F
CCTACAGTTTCCAAGGACAAGCAG
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
B6
003-054731501R
CCCAGCAGTAGCTTTAGCATCC
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
C5
016-071012605F
CTCCTAGCTTCCTGATTCTTTGCC
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
C6
016-071012605R
CCCACCCTGGTACAGCAAATG
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
D5
012-072604585F
GTTGAGAGTTGACACCAACTTCTTTTCTTTTTCC
S2_S5_NN_Ranking-TALENv2.0	Warning! Primer pair was generated using suboptimal parameters. 
D6
012-072604585R
CCACTCCTAGTGTGGAACCTC
S2_S5_NN_Ranking-TALENv2.0	Warning! Primer pair was generated using suboptimal parameters. 
E5
005-078702362F
GAAAGGATCCTGGATGAGGGG
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
E6
005-078702362R
GTAGGAGTTTCCTGCCTCTGTC
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
F5
020-045428486F
GTCTCACTGAGACCTAGGCG
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
F6
020-045428486R
TGGGCCTGGGGAACCAACAA
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
G5
007-003795640F
CCTGACTCCTTTCACATGGCATAG
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
G6
007-003795640R
TGTGTCACACGTGCTATGCCATG
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
H5
007-003795876F
GAGTCCTTTCACATGGCATAGCAC
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
H6
007-003795876R
ACATGTCTCACACGCACAATGCC
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
A7
002-130879063F
AAGACGCCCACCGAGCCCA
S2_S5_NN_Ranking-TALENv2.0	Warning! Primer pair was generated using suboptimal parameters. 
A8
002-130879063R
GCTAATGGGTGCAAAGTTTCTTTTAAGGAGCATA
S2_S5_NN_Ranking-TALENv2.0	Warning! Primer pair was generated using suboptimal parameters. 
B7
002-131974522F
GCTAATGGGTGCAAAGTTTCTTTTAAGGAGCATA
S2_S5_NN_Ranking-TALENv2.0	Warning! Primer pair was generated using suboptimal parameters. 
B8
002-131974522R
AAGACGCCCACCGAGCCCA
S2_S5_NN_Ranking-TALENv2.0	Warning! Primer pair was generated using suboptimal parameters. 
C7
008-015147764F
CCCCACTTCATCTGCTAAACTTCC
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
C8
008-015147764R
GAGGGGGAAAATGTGGGAAGTTCT
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
D7
014-077327823F
CCAACAGCTGGTAAGGATGTCAAG
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
D8
014-077327823R
CTGTGGAAACACCAAAGCAGGG
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
E7
004-118702135F
GCAGGGACACTGGTGTTATGTCT
S2_S5_NN_Ranking-TALENv2.0	Warning! Primer pair was generated using suboptimal parameters. 
E8
004-118702135R
GATAGTAACTTATTTAGAATTGAGGTTGTGTGATGGAG
S2_S5_NN_Ranking-TALENv2.0	Warning! Primer pair was generated using suboptimal parameters. 
F7
002-235805535F
ACCTCCACCAGACCACGAATG
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
F8
002-235805535R
GACCCTTAGCAGGTCACACTC
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
G7
010-111642202F
CTCGCTCACAGCATAGCTGG
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
G8
010-111642202R
TATGTGTGTCTGTGTTGCCACCTC
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
H7
002-241334168F
CATGCACCCCTGGCTTGTGT
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
H8
002-241334168R
AGCTTTGCACCTGGAACCTGTGA
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
A9
005-111863094F
AGTACACTTGGACCCAGCTTACC
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
A10
005-111863094R
TGAATCCCAGGTAGCGTACCTGC
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
B9
019-009837811F
AATCCCAGGTAGCGTACGTGC
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
B10
019-009837811R
TCAGTACACATGGACCTGGCTTC
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
C9
010-029082810F
CCTCTGAGCCCCATTCTCCT
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
C10
010-029082810R
TAGAGATGGGGTCTCACCTTGTTG
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
D9
015-100263695F
GACTGAGGAGAGACTGCTGTTC
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
D10
015-100263695R
GTGGAGACCTTAAGAGATGTCTCG
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
E9
012-040234817F
CCTTACAATTCACGGCTCTCAGAC
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
E10
012-040234817R
CCACTGGAGTTGACAGATTTGGC
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
F9
005-177943719F
AAGCTTTCTGCCTCCTCAGGCC
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
F10
005-177943719R
CACCTCAGTGACAGGCGCTT
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
G9
019-039625781F
CCTCACCAACATTTGGTACCGTC
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
G10
019-039625781R
CGGAGGTTGCAGTGAGCTGA
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
H9
002-219751327F
TGTGTCTTAACGAGCCGCCC
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
H10
002-219751327R
CTGACAGCTGTCATAGCCACG
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
A11
014-049949953F
GTTGTTCTTTCACCCACTGGAGC
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
A12
014-049949953R
GACACATCAGAAAGACCGCTGAC
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
B11
004-190659833F
CTGCCTCCAATCCCTCAGAAAATG
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
B12
004-190659833R
CTGGTATTGGGTCCAGACCAC
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
C11
012-101038644F
CTGTGCTCCCCTTTCTGAATAGTG
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
C12
012-101038644R
TACACTTGGACCCGACTTCCC
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
D11
00Y-018946879F
ATACACTTGGACTCGGCTTCCC
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
D12
00Y-018946879R
GTGAATCTCAGGTAGTGTACCTGC
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
E11
019-045727578F
GAGAAGGGGAGTGTTCCACTG
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
E12
019-045727578R
GGATAACAGAGGGCCATCAGC
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
F11
022-024555541F
CACAGCCATTCTGTCTACTCCC
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
F12
022-024555541R
TGGCAGGTGGCCAGTGTCTT
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
G11
001-110268884F
CAGGCAGGCAATCTTGATGTGG
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
G12
001-110268884R
CTCCTTTTTCCTGCGTAGCAAGTG
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
H11
011-031965225F
CCAGCCTGAGACAACCATCTTTAC
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
H12
011-031965225R
GAAGGGCCATAACTTGGGCATTAG
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
Primer Plate #2
A1
011-002239013F
AGCTGTCCAGCCTGCAGCAT
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
A2
011-002239013R
TCATATTCCTGGGTGGTCACTCC
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
B1
017-009569404F
GGGAATGAAAAGGACAGCAGGAC
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
B2
017-009569404R
CGGCCCTGCGCGTTATTTCT
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
C1
No PrimersF
No Primers
S2_S5_NN_Ranking-TALENv2.0	No Primers
C2
No PrimersR
No Primers
S2_S5_NN_Ranking-TALENv2.0	No Primers
D1
017-009569404F
GGGAATGAAAAGGACAGCAGGAC
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
D2
017-009569404R
CGGCCCTGCGCGTTATTTCT
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
E1
004-110007835F
GACTGCTGGGAATTGCTCTTTGAC
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
E2
004-110007835R
TCCCTCAACTTCCACTGGGC
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
F1
003-070683485F
GGGCAAAGGATCTGAATAGACAATTTCCCA
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
F2
003-070683485R
CACTGATGGACACTTCTGTTGCC
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
G1
005-172280139F
TGGTTTACCACGTGCTGTTGTTGC
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
G2
005-172280139R
AAGATGCCTCTGGGTGGCCT
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
H1
004-186747672F
CTGTGAGCTACTGAGGATATGCG
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
H2
004-186747672R
GGGCAGATGCTTAAATCACTCCCA
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
A3
014-024242484F
CAAGTCTTCCTCAGCCATCAGACA
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
A4
014-024242484R
CCAGACACATCAACAGAGTGGAAG
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
B3
003-052584959F
GGCTAGGAATCTTAAGCACCCC
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
B4
003-052584959R
GAGGGGATAGCAAGGCTGTG
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
C3
017-003965044F
CGTAAGTCCTGACAACTCTACCTC
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
C4
017-003965044R
GAAAGGCAGGACCCTTATCACTGT
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
D3
012-032161181F
GCTCAATGGCAGAAAACCAAATAACCCAAC
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
D4
012-032161181R
GAGACGGGTTTTTGCCATGTTGC
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
E3
006-153846994F
GCAAGCTGTCTCATGCTCACTG
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
E4
006-153846994R
CAGGAAGGAACTTGTCAGATTCCC
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
F3
011-006643499F
TGGGAAGCCATAGTCCCCAG
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
F4
011-006643499R
ATCAATGAGTTCCCCCGTGTGG
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
G3
001-230976433F
GCGCCAAGGTCTTCTTGAGC
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
G4
001-230976433R
GGAGAGTAGGAGACAGCACATG
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
H3
011-065255104F
TGGGGGAGAAAGGGAAAGGC
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
H4
011-065255104R
AGGCGGAGGCAGAGTAGGTA
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
A5
018-055555293F
TCTGGGAAGAAGCTGCCAGG
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
A6
018-055555293R
GGCTATGCTACCTGCTGTAAAACC
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
B5
011-012966658F
TGAGGGATAGAGCAGAGGATGG
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
B6
011-012966658R
GGGGGAATGATGTCACTGGAAC
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
C5
008-142398434F
GCCTGAGATAGGGACATTGGTG
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
C6
008-142398434R
CCCATTCTGAGCGAGCTTGGA
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
D5
004-002790263F
ATGCAGAGATGGCCCAGAGC
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
D6
004-002790263R
TGAGTAGGGCCAAGGCCACA
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
E5
008-011765812F
ATCCCCAGTAATGTACCTGCTGTC
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
E6
008-011765812R
CTCAGTACACTTGCACCCGG
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
F5
005-016519335F
CCTAGATCAGCGGCTTTCAAAGCA
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
F6
005-016519335R
GGGCTGAAAGGAGCTTTGAGG
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
G5
003-123352705F
GGTTTTAAGAAATGGGGGTCTCGC
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
G6
003-123352705R
TCCCACCAGAGTATCAGATGGC
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
H5
004-043460367F
GCTAAGAGCCTCTTGGGCTTTTG
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
H6
004-043460367R
AGCAGAGGCAGAAAGGACCAG
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
A7
003-060011649F
GTACTGCCAGAGTGTCAAGAAAGC
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
A8
003-060011649R
TAGGAAGAGGACAGTAGGCTAGG
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
B7
005-137519020F
GCGAGGATCAAAATGGCAATCCC
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
B8
005-137519020R
GGCAGAGGACTAATGTTTCCTGG
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
C7
006-101676033F
CCAGGTAACATACTTGCTGTCTGC
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
C8
006-101676033R
GTCACTACTCAGTACACTTGGACC
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
D7
015-090111045F
GCCTTCCTCTCCTCATACCC
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
D8
015-090111045R
TACAGGTGTGAGCCACCACG
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
E7
009-129960176F
CTTCCTTGGAGTGTCCAATGGG
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
E8
009-129960176R
CATCAGGGCTTTTGGCCTCAAAC
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
F7
00X-148285072F
GGAGCAAAAAGGACTATGTCACTGAGTCAA
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
F8
00X-148285072R
CCACGTAAGTCCTACAGGTGACTA
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
G7
004-132113714F
GCTGTTCTGAGGTTGTTCCTGTG
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
G8
004-132113714R
CCAGACTGAAGCATAGTCCCATGA
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
H7
003-099990183F
GTGGAGACGTTTTTGTGGTGGAC
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
H8
003-099990183R
CTCAGCCCCCTCCAAAAACAAAC
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
A9
003-138953648F
CTTGGTGTCTGAGTGGCAAAACTG
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
A10
003-138953648R
TATGGGCCTGTTTTACACGGGCCT
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
B9
008-122428182F
ACAAGTTCTGCCAACTACACCAGG
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
B10
008-122428182R
AGAGCCATTCCCAAGTGGGGT
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
C9
020-040489952F
GTGCCACCCACACAGCATTC
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
C10
020-040489952R
GCCAGCAATGTAGAGGGACAATG
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
D9
00X-079108050F
GGTGTCAGTAGCAGATAGGGATG
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
D10
00X-079108050R
CTGTACCCAACCCACTTTATGGC
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
E9
006-106935880F
TGGTGCATTGACTTTGGTTCTGGG
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
E10
006-106935880R
TCTGACCCAGACCTGACAGATAG
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
F9
003-030699112F
CTGGCACAGAGTGAGCACTTAATG
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
F10
003-030699112R
GGTCGTCATGAGGAGAACACCTT
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
G9
015-072957610F
TACTCACCTCTTGCCTTCAAGTGG
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
G10
015-072957610R
CTTTTCTTGGCCCATGCCAGG
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
H9
015-074364061F
TTGGCCCATGCCAGGACTCA
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
H10
015-074364061R
GCCCCTACAAGAATACTCACCTC
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
A11
015-075561464F
TACTCACCTCTTGCCTTCAAGTGG
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
A12
015-075561464R
TTCTTGGCCCATGCCAGGAC
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
B11
015-075585740F
TACTCACCTCTTGCCTTCAAGTGG
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
B12
015-075585740R
TTCTTGGCCCATGCCAGGAC
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
C11
015-076078165F
TACTCACCTCTTGCCTTCAAGTGG
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
C12
015-076078165R
CTTTTCTTGGCCCTTGCCAGGA
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
D11
018-046555916F
AAATGCACTTACGGGAGATGGCTG
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
D12
018-046555916R
GATGTCTCCCTTTCCCTATCCAAC
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
E11
002-170227380F
GGCCAGAAGACCAAGATGATTCC
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
E12
002-170227380R
GCCAAAAGCCAAATGTAGACTTTGTGCTG
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
F11
001-227379954F
GAGATCACTCTCACCACCCAAAAC
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
F12
001-227379954R
GGAAACAGCAGAACGTTGTTGGTG
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
G11
004-024170953F
CTGGATGGGATCCCAGAACAGA
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
G12
004-024170953R
GAGCCAACATGCTTGGCTGAG
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
H11
002-008374518F
TCCCGGAAACATGTCAGCCC
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
H12
002-008374518R
CATCCTACATCCCCTGAACATGG
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
Primer Plate #3
A1
009-094951264F
ATCCAGCCCTGGGACTTTACTTC
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
A2
009-094951264R
TGTGAGTCTCAGAACCAAGGCTGT
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
B1
011-027668392F
GGTGGGTCCATTCTGACTCTG
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
B2
011-027668392R
TCATGATCCACCCACCTCGG
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
C1
018-076246512F
AGCCTCCTACTGATGGGTGG
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
C2
018-076246512R
GCTGATGAAGAAGAGGATGGGG
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
D1
018-076246512F
AGCCTCCTACTGATGGGTGG
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
D2
018-076246512R
GCTGATGAAGAAGAGGATGGGG
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
E1
003-005393709F
CATCTACAGAATAACCCCAGGAGG
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
E2
003-005393709R
CCGTGTCACAGACAGTGTCTGAA
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
F1
009-117519299F
CAAAAGGACCTGCATCTAAGCACC
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
F2
009-117519299R
ATTGTTCTTCCCTGGCCACCC
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
G1
001-230749531F
CAAAGATGCAAAGGCTGATTTAGAGCACC
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
G2
001-230749531R
CCCCAAATTTAGCAATACTGTCTGCTGGA
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
H1
002-011376315F
CCCCTCATGGAGAACACAATACAAAAGAAAC
S2_S5_NN_Ranking-TALENv2.0	Warning! Primer pair was generated using suboptimal parameters. 
H2
002-011376315R
GAGAGAAGAGTTACGCGTCTTCC
S2_S5_NN_Ranking-TALENv2.0	Warning! Primer pair was generated using suboptimal parameters. 
A3
001-045422945F
GCCCCCAAACACCACAAAGTC
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
A4
001-045422945R
ATTACAGGCACATGCCACCACG
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
B3
010-133959783F
TTTCTCTCGGCACCTCCCAC
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
B4
010-133959783R
GTCCCTGCCCATGGACAAGT
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
C3
003-077451553F
CTGCACGTGTGTCCCAGAAC
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
C4
003-077451553R
ACACACGCTCACTTGAGGCATTC
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
D3
007-126235510F
GGGGTTTTGTCATGTTGCCCG
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
D4
007-126235510R
GCGACCAGCCGGACTATAAATC
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
E3
011-060049460F
GGGGACTTCATGTGCCGTAG
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
E4
011-060049460R
GGTCTGTCTTATCAAACGCACTGG
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
F3
004-182299969F
GACAAGGAAACTGAGGCACAGAAG
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
F4
004-182299969R
ACACTGATTCCTGACCTCAGCG
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
G3
011-056386094F
GGGAGGTACTATGCCTGGATG
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
G4
011-056386094R
GCTCGGGAGGATTAGTAAAAGAGG
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
H3
00X-070076863F
CAGGTGTGGTAAAGTGAACTGGG
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
H4
00X-070076863R
GACACTAGTGGCACTTGAAGGAG
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
A5
008-041570215F
GGGTGCAGAGAGATTTGCATGTG
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
A6
008-041570215R
TGTCTCCTCCTGTCCCTGCT
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
B5
001-118086389F
CTGTTCATTTTCTTAAGGTGGAGACTTACCTG
S2_S5_NN_Ranking-TALENv2.0	Warning! Primer pair was generated using suboptimal parameters. 
B6
001-118086389R
GCACTCTGGAAACATTCTCTTTCAATGTGTC
S2_S5_NN_Ranking-TALENv2.0	Warning! Primer pair was generated using suboptimal parameters. 
C5
006-088451104F
GATCTCCTTGAAGGCACATGCAG
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
C6
006-088451104R
GGCCTGGGAAACATAATGACACC
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
D5
016-059193695F
GTAGCCTCTCCCAATACTTAACGG
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
D6
016-059193695R
CCTGTGTTGTTTCTGTCCAAGGC
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
E5
016-007614298F
TTGGGACGCAGAGGTGGGTA
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
E6
016-007614298R
CTGGCTTGCAGATGAGTGCCT
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
F5
006-132465014F
GTTGTGTTGTGGCTGCTCAGAGT
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
F6
006-132465014R
CCTTCTATCCCCATCCTCTCTTTC
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
G5
007-004703855F
CTGCTGTCTCTATGGATTTGCCTG
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
G6
007-004703855R
CCACCTCCACACTTGGCCAAT
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
H5
019-022376173F
GGTACTCTCACTGTGGAAAACTGG
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
H6
019-022376173R
GGCCAATTCTCCTCTCCTTTTTCC
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
A7
016-087916217F
TGGGCCCAACATGTCAGTGC
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
A8
016-087916217R
GAGATCACACTTGGTGGCGATG
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
B7
006-048618042F
CCTCCTGTCTTTATTAGTCTAGCAAGAAGTTTGT
S2_S5_NN_Ranking-TALENv2.0	Warning! Primer pair was generated using suboptimal parameters. 
B8
006-048618042R
GTGGTGTCCTGGATAAAATCCTGAAACAGA
S2_S5_NN_Ranking-TALENv2.0	Warning! Primer pair was generated using suboptimal parameters. 
C7
002-086899302F
GGCTCTGTCTTTTGTTATGTCAAATGAGAAGCA
S2_S5_NN_Ranking-TALENv2.0	Warning! Primer pair was generated using suboptimal parameters. 
C8
002-086899302R
CTCCATCATTAATTCAAACAGCCTTCAATACCC
S2_S5_NN_Ranking-TALENv2.0	Warning! Primer pair was generated using suboptimal parameters. 
D7
001-008489463F
GGATAGAGAATACTGGGCTGAGAG
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
D8
001-008489463R
GTAAGAGGACTGAGTAGATGTCCC
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
E7
018-072086920F
GCTGTCACCAGGAAAAACATAAGAGCC
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
E8
018-072086920R
AAGCTGCTGAAATGGGCAGAGG
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
F7
001-213140398F
ATTCTAAGAGTGGCGTGTGGAGG
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
F8
001-213140398R
GTTGTGGGTGATTCCAATGGCATG
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
G7
012-047471859F
GTGGACCATCAGTCTTTCCCC
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
G8
012-047471859R
CTGAGAGGCATCTACCTTCATGG
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
H7
001-109847447F
GCAGGCTTGCCAAACTAAAAGCTC
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
H8
001-109847447R
TCAGAGAGCCAACAGAGCCC
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
A9
010-107873570F
GACACCTTCTGCTAAGCACACATC
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
A10
010-107873570R
GAGCTCATTCAAAGCCCCAGTAG
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
B9
001-011023947F
TTTAATCCCCCCTCCCCTCC
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
B10
001-011023947R
AAAGCGTCCACTGTGGGCCCAT
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
C9
016-083864892F
GGAAACTGTGGCCCAGAGAG
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
C10
016-083864892R
CTCAGCATCCGTTCCTGTTCTTC
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
D9
005-083704636F
GGAACGGATTAAAGGGAAGCCAG
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
D10
005-083704636R
GGTCTGTTTCTGTAACATCAGAGGCAAAG
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
E9
010-052784032F
ACTGAGGCCCAAATCATAGCCAATGATGGA
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
E10
010-052784032R
AGGCCTCCACAGCCATGCTT
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
F9
016-068387900F
GCGCCAGTGGTCCTTGGATT
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
F10
016-068387900R
CTGGGAATACAGGTGTGCACTAC
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
G9
005-078059903F
CTGCTCAGCTCATCAGAACACTC
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
G10
005-078059903R
GGACAGAAGCTTGTTGAGTGGTG
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
H9
005-019467940F
GAGATCAGGCCACAGTCATATTCC
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
H10
005-019467940R
CCTGGCGACAGTTGTCATCATC
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
A11
004-016836013F
CACCTATGTGGCGTGATTGAGTGA
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
A12
004-016836013R
AGACAGGGTTTCAGCATGTTGGC
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
B11
020-062498891F
CCTGGGTGACAGAGTAAGACTCG
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
B12
020-062498891R
CCAATATGGTGAAACCCCGTCTC
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
C11
004-085199195F
CAGTTCTACTTCAGGGAAGGGG
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
C12
004-085199195R
GGATACAAGTGGAAAAGCACAGCC
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
D11
005-143474515F
CCACTCTCCCTCAAATCAACTGC
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
D12
005-143474515R
CTGCATTTCTGAGACAGACATGGC
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
E11
017-072588236F
CAGAGCGTCTCTGTCTCCAAG
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
E12
017-072588236R
GGCACACCTTGCAACATACACTG
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
F11
004-150284205F
GAGGAAGTCCCCAGTGAGAAAATG
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
F12
004-150284205R
TATTCCTGGACCCACCCTACTTC
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
G11
003-010453381F
TGTTGCAGACACTGGAACAGAGC
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
G12
003-010453381R
CGTGAGAGTGGACTTGCAAGTCTG
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
H11
013-067512369F
GGCATCAACAGGGCTTCTTAAACG
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
H12
013-067512369R
CAATGTCAGTCGTTTCTGCATGAAGATAGG
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
Primer Plate #4
A1
00X-088338909F
CCCCCCCAGAAAAGTGGTTTATG
S2_S5_NN_Ranking-TALENv2.0	Warning! Primer pair was generated using suboptimal parameters. 
A2
00X-088338909R
CAGATTTGGCTCACAGTAGCATACTTTACTC
S2_S5_NN_Ranking-TALENv2.0	Warning! Primer pair was generated using suboptimal parameters. 
B1
020-059992627F
TGGCCCTGTTTCTGGCTGGA
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
B2
020-059992627R
AGTACACTGAAGTGCAGTCTCTGG
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
C1
002-009410094F
CCTCGGTAACTTGAGAGGAACCTA
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
C2
002-009410094R
AAAACTCCACTCCAGCTCAGAGCG
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
D1
014-075136937F
GAGGATCAGAGCCCATCCAAATGT
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
D2
014-075136937R
GGAACCTGACAACCTGTGTCCT
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
E1
00X-073723246F
GTACAGTGGGGAGTCTGGAG
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
E2
00X-073723246R
CAGAAGACCAGAAGTAGGCCTAAC
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
F1
009-134936820F
GCTGGTAAGTGAAGTTGATGTGGC
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
F2
009-134936820R
CCAGGAGCAGCTGCGATGAA
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
G1
022-036626609F
CTGACTCCAGGCTCTGACAC
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
G2
022-036626609R
GCTGGGGCCTTTTTTCTCATCTG
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
H1
007-073021712F
GATGCGCCGCTTCCAGTAGT
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
H2
007-073021712R
TGTGAAGCGGAGGAAGAGCC
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
A3
001-024242432F
TCCCAAGAGCAAGACATTGGCAG
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
A4
001-024242432R
GCAATGGAGGACCAGGCGTT
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
B3
215-000126245F
GTCCCCTCTCCTTGCACTTTTC
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
B4
215-000126245R
CCTTGGGAACCTTGGACAATAAGC
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
C3
021-015154271F
GTCCCCTCTCCTTGCACTTTTC
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
C4
021-015154271R
CCTTGGGAACCTTGGACAATAAGC
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
D3
006-024980600F
GGGAGTGAAATCAAGACACCTGG
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
D4
006-024980600R
AATGCCCAAAAGCCCCCTGTG
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
E3
005-154638956F
GGGCAACAAGAGTGAGACTCTG
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
E4
005-154638956R
CGTGTTCCACAAGATGGGCTG
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
F3
013-073489472F
GCGCTCTGCTTCTAAGACACATTG
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
F4
013-073489472R
CACATGGAAGAGATGATACATGCCATTACC
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
G3
017-068378451F
TGGGCTTCCTCCATGTTGGTC
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
G4
017-068378451R
ATGACAGGACCTGGCTCACC
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
H3
007-030006854F
CCAAGAAGGTTATGCCCTGAATGC
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
H4
007-030006854R
TAGTGGGTGTCCTTGGCTCTG
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
A5
010-085871707F
AGTACACTTGGACCCGGCTTC
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
A6
010-085871707R
TGAATCCCAGGTAGCGTACCTGC
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
B5
006-071979511F
GACATTCTAGAGCAGTTTGGCAGG
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
B6
006-071979511R
GTGACTGTGGGTGAAGATGCTG
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
C5
010-100332368F
GAGAGTGAACTCACCTTCCATGAC
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
C6
010-100332368R
TGGGCACACAGTCAGTTCCTAG
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
D5
017-068268613F
AGGAAGGGATTCTCCTAATAAATTGGCAGATG
S2_S5_NN_Ranking-TALENv2.0	Warning! Primer pair was generated using suboptimal parameters. 
D6
017-068268613R
CTATGGTCCATGTCTAACGTTAATACTACATGC
S2_S5_NN_Ranking-TALENv2.0	Warning! Primer pair was generated using suboptimal parameters. 
E5
016-048484957F
CCAGGGAAGCATCATGCCCTTT
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
E6
016-048484957R
CAGCCTGCAGAATGGAAGGG
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
F5
00X-136731121F
GTCACTTTACCTCCCTGAACCTC
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
F6
00X-136731121R
AGACAGCATTTCGCCATGTTGACC
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
G5
006-027625538F
ATCCGAGAACTGTGGGAGGC
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
G6
006-027625538R
CTCCCTGTTAATGCCACCCC
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
H5
011-117354866F
GCTCAGGCAAAGTTGTTCCCAG
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
H6
011-117354866R
ATGTGCTGAGAGGCTGGTGC
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
A7
003-052213069F
CATAGGGGTGAGCTGAGCTCAT
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
A8
003-052213069R
GGCCTTTGGAAGGAGAATGGCT
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
B7
009-096658949F
AGAGAGAGGCCCTGGAAGGT
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
B8
009-096658949R
TTCCTAGGCCTTGGCCCTCT
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
C7
001-242741404F
CTTGAAGGCTCCAACTTCATGTCC
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
C8
001-242741404R
CATTCTGGGCTCTTCATAAGGCTC
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
D7
015-079604259F
TTGGAAAGGAGGGATCAGTGCAG
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
D8
015-079604259R
GTGCCTATCAGCATGCGTCC
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
E7
008-078830967F
CAGGAATGTCAGGTGACTCTCAG
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
E8
008-078830967R
CACTGGAACTATGCACATGCTCAC
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
F7
003-116802161F
GGGATGGTCCAAAACTCAAACCTC
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
F8
003-116802161R
GAAGGCATTGGGGCTAGACAAG
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
G7
010-075583632F
GGTATAATGCCCTTCCTCAGCATG
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
G8
010-075583632R
CAGGGAAGAGCCATAGTCTGTC
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
H7
001-184575055F
GGTGGTGCTAAACCGAACCTG
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
H8
001-184575055R
GCCACTACACCTGGCCAATG
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
A9
005-144614657F
CAGTGACCTTAGCAGACTATCAGC
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
A10
005-144614657R
CTCTGGGCTTGACAGCAACTGA
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
B9
007-044570049F
GGAGATGGATGGTGGTGATAGTTG
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
B10
007-044570049R
TTAGGCAGTACTGGCCTGGG
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
C9
012-129191997F
GGTGGGGTATACCAGCATAAACTC
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
C10
012-129191997R
GGCTTCAAGGACCCTTTCTTAGCT
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
D9
003-183756342F
AGGGAGATGCTGTCCCACTG
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
D10
003-183756342R
TCCATTCTGAGCCCCCTGTC
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
E9
003-183824037F
CCTGCTGCTCCACTGCAACA
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
E10
003-183824037R
TCCATTCTGAGCCCCCTGTC
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
F9
012-059855477F
GAGAAAGGATTGAGTATTTTTCTCAGGACTTGG
S2_S5_NN_Ranking-TALENv2.0	Warning! Primer pair was generated using suboptimal parameters. 
F10
012-059855477R
CAGAATTTCAGAGGCCCTCCATC
S2_S5_NN_Ranking-TALENv2.0	Warning! Primer pair was generated using suboptimal parameters. 
G9
010-006225239F
CGCCAACTTTGGGGTTGGCT
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
G10
010-006225239R
AAGTGCTGGGATTACAGGCATGAG
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
H9
017-045024202F
CTGGGTGACGAAATGAGACTCTG
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
H10
017-045024202R
TTCATGTCAGGGTCCCCTGG
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
A11
014-086334283F
CACACACACACACACCCTTTGATC
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
A12
014-086334283R
CAGCAACGCTGACCTAGAAACTC
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
B11
012-006059468F
GGTAAGAGGAAAGGCGAAGTCATG
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
B12
012-006059468R
CAGGGCCTTAAATCCACAGCAAC
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
C11
014-100782711F
TGAAGGGACAGAGCCAGGGT
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
C12
014-100782711R
ACACCAGCCTGGGCAACAGA
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
D11
003-157302016F
ATCCCAGGTAGCGTACCTGC
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
D12
003-157302016R
CTTAGTACACTTGGACCTGGCTTC
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
E11
020-039770399F
CCAGCTGGGATCCTCTGTTTC
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
E12
020-039770399R
CCTAGCACTGGGCAAATGGG
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
F11
006-138657515F
GTTCCCGTGCATCAGTCAGC
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
F12
006-138657515R
GCTGATCAGTGGTTGGAAAGGC
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
G11
001-054689628F
GTGAAGGCAACTGCCAGGCT
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
G12
001-054689628R
GGCTTTTGCTGAGTACAGACACC
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
H11
002-097493184F
TAGTAGAGGCGGGGTTTTGCCA
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
H12
002-097493184R
AAAAAGGGACAGTGTCCACTGGG
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
Primer Plate #5
A1
002-061428882F
GGTTAAAGCTGTCCGAGTAGCTAG
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
A2
002-061428882R
GCTGGTTCCTGGTAACCTGG
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
B1
018-074790610F
GAGCTGTGGACTGAAGAGCAAC
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
B2
018-074790610R
CACAGTCTTGGGAGGGTGCT
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
C1
009-034728408F
TTCATTTCCAGGGCATTGAGGTACAGG
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
C2
009-034728408R
GCAAAGGAAGTCCAGGAGAGTG
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
D1
019-017971876F
CTCAATTAATCCTCCCTGGAGACC
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
D2
019-017971876R
ATTCGCATGCGGTAGAGGGG
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
E1
016-088575852F
CCATTAGGCACACAGGGAGG
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
E2
016-088575852R
CTGCCTCCTGAACCCACTCT
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
F1
002-241889867F
TCAGAGCCTGAAGCAGGAGC
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
F2
002-241889867R
GGGCAAGTGGATGGGTGGAT
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
G1
005-131322201F
CTGCATCTTCTGGAGAGTTTGCC
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
G2
005-131322201R
TTGAGCGAGATGGCGTGCAG
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
H1
001-203281134F
TGCATGCTGCAGATTAAGAGCCC
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
H2
001-203281134R
TAAGAAGTACCAGGACCACAACCC
S2_S5_NN_Ranking-TALENv2.0	Primer pair generated using optimal parameters.