PROGNOS Summary for S2_S5_NN_Ranking-RVDs_5TC

The hg19 genome was searched for the TALEN pair targeting:
5'- TCACCTTGCCCCACAGGGCAGT NN..NN ACAGGAGTCAGGTGCA -3'

Using these RVDs:
Left:  01HD02NI03HD04HD05NG06NG07NN08HD09HD10HD11HD12NI13HD14NI15NN16NN17NN18HD19NI20NN21NG
Right: 01NN02HD03NI04HD05HD06NG07NN08NI09HD10NG11HD12HD13NG14NN15NG

Up to 5 mismatches were allowed in each nuclease half-site.

Allowed spacing distances were: 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30

Homodimers and heterodimers of the nucleases were included in the output

4067 total sites were located
Mismatch Types
Genomic Regions
  0 and 2: 1
  2 and 2: 1
  3 and 3: 5
  2 and 5: 12
  3 and 4: 21
  3 and 5: 126
  4 and 4: 69
  4 and 5: 937
  5 and 5: 2895
  Exons: 190
  Promoters: 33
  Introns: 1633
  Intergenic: 2211


The top 200 sites are ranked below according to a weighting of the RVD base preferences. All sites listed contain flanking 5' pyrimidines.
For the full output, click here.

This output will be deleted from the server one week after it is generated.  Please save your results.

Primer pairs were designed using the 400 bp of sequence on either side of the cleavage site to melt close to 60 °C

For easy visualization of cleavage on gels:
The smallest fragment will be longer than 130 bp, which is also the minimum separation distance between uncleaved and cleaved products.
The two cleavage products will be separated by a minimum size of 0 bp
To optimize assay conditions, the amplicons have a minimum length of 260 bp, a maximum length of 450 bp, and an optimal length of 270 bp.
Rank: 1	Homology Score: 52	RVD Match Score: 2.328	Left Half-Site RVD Score: 3.661	Right Half-Site RVD Score: 4.49
	Genomic Region: Exon	Closest Gene: HBB	Caution! This site scores very closely to the intended nuclease target. The potential for cleavage at this site is of great concern.
Match Type: L16R
	           Left Nuclease
	         TCACCTTGCCCCACAGGGCAGT
	5'-CACGT TCACCTTGCCCCACAGGGCAGT        AACGGCAGACTTCTCC              TCAGGAGTCAGATGCA CCATG-3'
	3'-GTGCA AGTGGAACGGGGTGTCCCGTCA        TTGCCGTCTGAAGAGG              AGTCCTCAGTCTACGT GGTAC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 011-005248069
Forward Primer: 5'-GTGCCTATCAGAAACCCAAGAGTC-3' Tm: 59.038
Reverse Primer: 5'-CAATCTACTCCCAGGAGCAGG-3' Tm: 59.378
Expected PCR Product Length: 308
Expected CelI Cleavage Product Sizes: 154, 154
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr11:5248194-5248247

Rank: 2	Homology Score: 32	RVD Match Score: 2.561	Left Half-Site RVD Score: 5.563	Right Half-Site RVD Score: 4.49
	Genomic Region: Exon	Closest Gene: HBD
Match Type: L16R
	           Left Nuclease
	         TCACCTTGCCCCACAGGGCAGT
	5'-CACGT TCACTTTGCCCCACAGGGCATT        GACAGCAGTCTTCTCC              TCAGGAGTCAGATGCA CCATG-3'
	3'-GTGCA AGTGAAACGGGGTGTCCCGTAA        CTGTCGTCAGAAGAGG              AGTCCTCAGTCTACGT GGTAC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 011-005255477
Forward Primer: 5'-GTCAGTGCCTATCAGAAACCCAAG-3' Tm: 59.038
Reverse Primer: 5'-TCACTGGAGCAGGGAGGACA-3' Tm: 59.508
Expected PCR Product Length: 296
Expected CelI Cleavage Product Sizes: 158, 138
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr11:5255606-5255659

Rank: 3	Homology Score: 10	RVD Match Score: 2.626	Left Half-Site RVD Score: 3.74	Right Half-Site RVD Score: 5.088
	Genomic Region: Intergenic	Closest Gene: IARS
Match Type: R23R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-ACCCC CGCACCTCACTCCAGT          ATCCCAGGGAGAGAGATTCTCAG           GCAGGAGTCTGTTGTG CTGTG-3'
	3'-TGGGG GCGTGGAGTGAGGTCA          TAGGGTCCCTCTCTCTAAGAGTC           CGTCCTCAGACAACAC GACAC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 009-094951264
Forward Primer: 5'-ATCCAGCCCTGGGACTTTACTTC-3' Tm: 59.148
Reverse Primer: 5'-TGTGAGTCTCAGAACCAAGGCTGT-3' Tm: 59.038
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 135, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr9:94951370-94951424

Rank: 4	Homology Score: 10	RVD Match Score: 2.7	Left Half-Site RVD Score: 5.479	Right Half-Site RVD Score: 3.87
	Genomic Region: Intergenic	Closest Gene: FAM47A
Match Type: R10R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-AGTCT TCACCCTTACTCCTAT                 GCTTATTCTA                 ACAGGAGTTAGATGTA CTTCT-3'
	3'-TCAGA AGTGGGAATGAGGATA                 CGAATAAGAT                 TGTCCTCAATCTACAT GAAGA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 00X-034076224
Forward Primer: 5'-AGTATTGCCCTTTCTGCTAGGGAC-3' Tm: 59.038
Reverse Primer: 5'-CACTGCACACTTCTGTGGCTC-3' Tm: 59.378
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 134, 136
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chrX:34076335-34076376

Rank: 5	Homology Score: 10	RVD Match Score: 2.706	Left Half-Site RVD Score: 4.597	Right Half-Site RVD Score: 4.72
	Genomic Region: Intergenic	Closest Gene: PLXNA1
Match Type: R28R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TGACT TGCAGCTCACTCCTCT        GTTCCTCTGCCACCCTGGACAGGACGGC        AGAGTGGTCAGGTGTG CCTCT-3'
	3'-ACTGA ACGTCGAGTGAGGAGA        CAAGGAGACGGTGGGACCTGTCCTGCCG        TCTCACCAGTCCACAC GGAGA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 003-126892267
Forward Primer: 5'-ACCCAGACCCAGACATCCAC-3' Tm: 59.508
Reverse Primer: 5'-CTATGTGCAGCTGGAGAAACTGAG-3' Tm: 59.038
Expected PCR Product Length: 282
Expected CelI Cleavage Product Sizes: 152, 130
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr3:126892387-126892446

Rank: 6	Homology Score: 2	RVD Match Score: 2.712	Left Half-Site RVD Score: 4.713	Right Half-Site RVD Score: 4.645
	Genomic Region: Intergenic	Closest Gene: NEK10
Match Type: R11R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-AGAGC CACACCTAACTGCTGA                CTGGGATAGGA                 AGAGGAGGCAGGGGTG TGGTC-3'
	3'-TCTCG GTGTGGATTGACGACT                GACCCTATCCT                 TCTCCTCCGTCCCCAC ACCAG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 003-027142836
Forward Primer: 5'-AGGACAGGAAGGAAGAGCCC-3' Tm: 59.508
Reverse Primer: 5'-GTGTGGATTGGCAACCTCTTCATG-3' Tm: 59.038
Expected PCR Product Length: 289
Expected CelI Cleavage Product Sizes: 132, 157
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr3:27142945-27142987

Rank: 7	Homology Score: 18	RVD Match Score: 2.721	Left Half-Site RVD Score: 3.85	Right Half-Site RVD Score: 5.662
	Genomic Region: Intergenic	Closest Gene: PKIB
Match Type: R29R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TTTAG CACACCTGACTGCTGT       GGAGTGTCCCAGCAGAAGGTTGTCCACGT        ACAGGAGCAAGATGCA GCCTA-3'
	3'-AAATC GTGTGGACTGACGACA       CCTCACAGGGTCGTCTTCCAACAGGTGCA        TGTCCTCGTTCTACGT CGGAT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 006-123066634
Forward Primer: 5'-GTAGTGGGATCGTGCCTGTG-3' Tm: 59.508
Reverse Primer: 5'-TCAGTACACTTGGACCCGGC-3' Tm: 59.508
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 135, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr6:123066737-123066797

Rank: 8	Homology Score: 8	RVD Match Score: 2.723	Left Half-Site RVD Score: 3.821	Right Half-Site RVD Score: 5.707
	Genomic Region: Intergenic	Closest Gene: HSBP1
Match Type: R14R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CGGTA CACACCACACTCCTGT               CTCTCGATGCCATT               GGAGGAGGCAGATGCA TCCGG-3'
	3'-GCCAT GTGTGGTGTGAGGACA               GAGAGCTACGGTAA               CCTCCTCCGTCTACGT AGGCC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 016-083864892
Forward Primer: 5'-GGAAACTGTGGCCCAGAGAG-3' Tm: 59.508
Reverse Primer: 5'-CTCAGCATCCGTTCCTGTTCTTC-3' Tm: 59.148
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 135, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr16:83865002-83865047

Rank: 9	Homology Score: 18	RVD Match Score: 2.728	Left Half-Site RVD Score: 4.957	Right Half-Site RVD Score: 4.522
	Genomic Region: Intron	Closest Gene: PHF21B
Match Type: R12R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CATTC TCCACCTGTCTCCTGC                CTCTCAACTGGG                TCTGGAGTCAGGTGGA AAAAT-3'
	3'-GTAAG AGGTGGACAGAGGACG                GAGAGTTGACCC                AGACCTCAGTCCACCT TTTTA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 022-045287459
Forward Primer: 5'-CAAAGGCTCTGCCTGCAGGA-3' Tm: 59.508
Reverse Primer: 5'-CAGGACTAAGGCCGCTCTTC-3' Tm: 59.508
Expected PCR Product Length: 269
Expected CelI Cleavage Product Sizes: 136, 133
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr22:45287571-45287614

Rank: 10	Homology Score: 5	RVD Match Score: 2.737	Left Half-Site RVD Score: 4.189	Right Half-Site RVD Score: 5.379
	Genomic Region: Intron	Closest Gene: ZC3H3
Match Type: R23R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-AGGCA CACACCTGACACCTGC          ACTGGGCAGCACCTTCGAGGCAA           AGGGGAGTGAGCTGCG CTGCG-3'
	3'-TCCGT GTGTGGACTGTGGACG          TGACCCGTCGTGGAAGCTCCGTT           TCCCCTCACTCGACGC GACGC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 008-144606723
Forward Primer: 5'-CTAGCAGCAGGCAAGTCGCA-3' Tm: 59.508
Reverse Primer: 5'-ACACAGAGCATGGGCTTGGG-3' Tm: 59.508
Expected PCR Product Length: 303
Expected CelI Cleavage Product Sizes: 130, 173
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr8:144606824-144606878

Rank: 11	Homology Score: 17	RVD Match Score: 2.745	Left Half-Site RVD Score: 4.174	Right Half-Site RVD Score: 5.455
	Genomic Region: Intergenic	Closest Gene: GPR6
Match Type: R30R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CCATC TCCACCTGGCTCCTGT       GTGGTCCATGTTGCAAGTTTTCAGGAAGGA       GCAGGAGTTAAGGGTA GAGTT-3'
	3'-GGTAG AGGTGGACCGAGGACA       CACCAGGTACAACGTTCAAAAGTCCTTCCT       CGTCCTCAATTCCCAT CTCAA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 006-110284189
Forward Primer: 5'-GCAATGTTGCTCCAAGAGGCC-3' Tm: 59.378
Reverse Primer: 5'-GAACTTATTCCTCCCACCTAGCTG-3' Tm: 59.038
Expected PCR Product Length: 297
Expected CelI Cleavage Product Sizes: 131, 166
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr6:110284287-110284348

Rank: 12	Homology Score: 17	RVD Match Score: 2.765	Left Half-Site RVD Score: 3.85	Right Half-Site RVD Score: 5.996
	Genomic Region: Intergenic	Closest Gene: TRHDE-AS1
Match Type: R28R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CCTCC TAGACCTGACTCCTGT        ATTTGTTTCCTCACACTTAAAGGGAACT        CAATGAGTCAGGTGAG TACTG-3'
	3'-GGAGG ATCTGGACTGAGGACA        TAAACAAAGGAGTGTGAATTTCCCTTGA        GTTACTCAGTCCACTC ATGAC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Warning! Primer pair was generated using suboptimal parameters. 
Primer Index: 012-072604585
Forward Primer: 5'-GTTGAGAGTTGACACCAACTTCTTTTCTTTTTCC-3' Tm: 59.668
Reverse Primer: 5'-CCACTCCTAGTGTGGAACCTC-3' Tm: 59.378
Expected PCR Product Length: 393
Expected CelI Cleavage Product Sizes: 263, 130
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr12:72604816-72604875

Rank: 13	Homology Score: 10	RVD Match Score: 2.782	Left Half-Site RVD Score: 5.716	Right Half-Site RVD Score: 4.189
	Genomic Region: Intron	Closest Gene: LOC100507605
Match Type: R27R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TCTGG CGCAACTCACCCCTGG        CTTGCACCAAACATTCTTAAAATGTTT         ATTGGGGTCAGGTGCA ATGGC-3'
	3'-AGACC GCGTTGAGTGGGGACC        GAACGTGGTTTGTAAGAATTTTACAAA         TAACCCCAGTCCACGT TACCG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 010-029082810
Forward Primer: 5'-CCTCTGAGCCCCATTCTCCT-3' Tm: 59.508
Reverse Primer: 5'-TAGAGATGGGGTCTCACCTTGTTG-3' Tm: 59.038
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 135, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr10:29082914-29082972

Rank: 14	Homology Score: 18	RVD Match Score: 2.782	Left Half-Site RVD Score: 4.867	Right Half-Site RVD Score: 4.984
	Genomic Region: Intron	Closest Gene: FAM3D
Match Type: R17R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CCCTG TGCCCCTGACTCCTTA             CAGCTGTTCTAGAATGT              AAATGAGGCAGGTGCA TTTCG-3'
	3'-GGGAC ACGGGGACTGAGGAAT             GTCGACAAGATCTTACA              TTTACTCCGTCCACGT AAAGC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 003-058640715
Forward Primer: 5'-CATATGAGGAAACTGAGAGGCGG-3' Tm: 59.148
Reverse Primer: 5'-CCTCGAGGAGGGCTATTCTAGA-3' Tm: 59.258
Expected PCR Product Length: 283
Expected CelI Cleavage Product Sizes: 130, 153
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr3:58640819-58640867

Rank: 15	Homology Score: 13	RVD Match Score: 2.782	Left Half-Site RVD Score: 5.228	Right Half-Site RVD Score: 4.632
	Genomic Region: Intergenic	Closest Gene: FAM41AY1
Match Type: R29R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TAGGC TGCATCTTACTCCAGT       ACATAGACAACCTTCTGCTAGGATACTCC        ACAGCAGTCGGGTGTG CAAGA-3'
	3'-ATCCG ACGTAGAATGAGGTCA       TGTATCTGTTGGAAGACGATCCTATGAGG        TGTCGTCAGCCCACAC GTTCT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 00Y-018946879
Forward Primer: 5'-ATACACTTGGACTCGGCTTCCC-3' Tm: 59.258
Reverse Primer: 5'-GTGAATCTCAGGTAGTGTACCTGC-3' Tm: 59.038
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 135, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chrY:18946982-18947042

Rank: 16	Homology Score: 13	RVD Match Score: 2.8	Left Half-Site RVD Score: 4.387	Right Half-Site RVD Score: 5.628
	Genomic Region: Intergenic	Closest Gene: HYDIN2
Match Type: R26R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CCCTC TGCACACAACTCCTAT         GCACTCTGCACGCACCACCCTCCTGC         ACAGGTGTCCCGTGCA GGCTG-3'
	3'-GGGAG ACGTGTGTTGAGGATA         CGTGAGACGTGCGTGGTGGGAGGACG         TGTCCACAGGGCACGT CCGAC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: dom-000216374
Forward Primer: 5'-TCTCCTTTCTGTACCTCAGAGACC-3' Tm: 59.038
Reverse Primer: 5'-CCCACCCTGGTACAGCAAATG-3' Tm: 59.378
Expected PCR Product Length: 318
Expected CelI Cleavage Product Sizes: 131, 187
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr1_gl000192_random:216474-216531

Rank: 17	Homology Score: 18	RVD Match Score: 2.811	Left Half-Site RVD Score: 4.571	Right Half-Site RVD Score: 5.505
	Genomic Region: Intron	Closest Gene: PDE4D
Match Type: R29R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CACAC TGCACCTAACATCTGT       CCTTATAATAAACTAGGAGCATCTCTGTT        ACAGGAATCATGTTCA GTAGT-3'
	3'-GTGTG ACGTGGATTGTAGACA       GGAATATTATTTGATCCTCGTAGAGACAA        TGTCCTTAGTACAAGT CATCA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 005-059619945
Forward Primer: 5'-CCAAGACTTAGCTTAGCACCCAAC-3' Tm: 59.038
Reverse Primer: 5'-CATGCCTGGAGTTAGCCTCTCT-3' Tm: 59.258
Expected PCR Product Length: 384
Expected CelI Cleavage Product Sizes: 219, 165
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr5:59620132-59620192

Rank: 18	Homology Score: 10	RVD Match Score: 2.816	Left Half-Site RVD Score: 5.523	Right Half-Site RVD Score: 4.592
	Genomic Region: Intron	Closest Gene: C14orf166B
Match Type: R24R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TAACT TGCCCCCAACTCATCT          TTTCTTAGATTAGCTCTGGCAATC          ACAGGAGGCAGATGTA ACAGT-3'
	3'-ATTGA ACGGGGGTTGAGTAGA          AAAGAATCTAATCGAGACCGTTAG          TGTCCTCCGTCTACAT TGTCA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 014-077327823
Forward Primer: 5'-CCAACAGCTGGTAAGGATGTCAAG-3' Tm: 59.038
Reverse Primer: 5'-CTGTGGAAACACCAAAGCAGGG-3' Tm: 59.258
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 133, 137
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr14:77327926-77327981

Rank: 19	Homology Score: 10	RVD Match Score: 2.817	Left Half-Site RVD Score: 3.821	Right Half-Site RVD Score: 6.445
	Genomic Region: Intron	Closest Gene: COL23A1
Match Type: R29R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-ATCCC TGCACCTCACACCTAT       CCTGAAGCAAACTAATCAGTAGTTAAATA        AGAGGAGGAGGGTACA TTACG-3'
	3'-TAGGG ACGTGGAGTGTGGATA       GGACTTCGTTTGATTAGTCATCAATTTAT        TCTCCTCCTCCCATGT AATGC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 005-177943719
Forward Primer: 5'-AAGCTTTCTGCCTCCTCAGGCC-3' Tm: 61.288
Reverse Primer: 5'-CACCTCAGTGACAGGCGCTT-3' Tm: 59.508
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 137, 133
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr5:177943824-177943884

Rank: 20	Homology Score: 17	RVD Match Score: 2.819	Left Half-Site RVD Score: 6.157	Right Half-Site RVD Score: 4.064
	Genomic Region: Exon	Closest Gene: LOC390660
Match Type: R27R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CTGCC CGCCGCTGCCACCTGT        GGCCCTGTGCCACCTGGCACTCAGGCA         ACTGGAGTAAGGTGCA TGAGG-3'
	3'-GACGG GCGGCGACGGTGGACA        CCGGGACACGGTGGACCGTGAGTCCGT         TGACCTCATTCCACGT ACTCC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 015-082621505
Forward Primer: 5'-TGGCTTTCACGGGTCTGTGC-3' Tm: 59.508
Reverse Primer: 5'-TAGACATGCAGCCTGGAGCC-3' Tm: 59.508
Expected PCR Product Length: 296
Expected CelI Cleavage Product Sizes: 157, 139
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr15:82621631-82621689

Rank: 21	Homology Score: 17	RVD Match Score: 2.819	Left Half-Site RVD Score: 6.157	Right Half-Site RVD Score: 4.064
	Genomic Region: Exon	Closest Gene: LOC390660
Match Type: R27R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CTGCC CGCCGCTGCCACCTGT        GGCCCTGTGCCACCTGGCACTCAGGCA         ACTGGAGTAAGGTGCA TGAGG-3'
	3'-GACGG GCGGCGACGGTGGACA        CCGGGACACGGTGGACCGTGAGTCCGT         TGACCTCATTCCACGT ACTCC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 015-082918832
Forward Primer: 5'-TGGCTTTCACGGGTCTGTGC-3' Tm: 59.508
Reverse Primer: 5'-TAGACATGCAGCCTGGAGCC-3' Tm: 59.508
Expected PCR Product Length: 296
Expected CelI Cleavage Product Sizes: 157, 139
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr15:82918958-82919016

Rank: 22	Homology Score: 17	RVD Match Score: 2.819	Left Half-Site RVD Score: 4.064	Right Half-Site RVD Score: 6.157
	Genomic Region: Intergenic	Closest Gene: LOC642423
Match Type: R27R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CCTCA TGCACCTTACTCCAGT        TGCCTGAGTGCCAGGTGGCACAGGGCC         ACAGGTGGCAGCGGCG GGCAG-3'
	3'-GGAGT ACGTGGAATGAGGTCA        ACGGACTCACGGTCCACCGTGTCCCGG         TGTCCACCGTCGCCGC CCGTC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 015-085803797
Forward Primer: 5'-TAGACATGCAGCCTGGAGCC-3' Tm: 59.508
Reverse Primer: 5'-TGGCTTTCACGGGTCTGTGC-3' Tm: 59.508
Expected PCR Product Length: 296
Expected CelI Cleavage Product Sizes: 142, 154
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr15:85803908-85803966

Rank: 23	Homology Score: 18	RVD Match Score: 2.829	Left Half-Site RVD Score: 5.662	Right Half-Site RVD Score: 4.551
	Genomic Region: Intergenic	Closest Gene: FLJ11235
Match Type: R29R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TAGGC TGCATCTTGCTCCTGT       ACATGGATGACCTTCTGCTGGGACACTCC        ACGGCAGTCAGGTGCG CAAAA-3'
	3'-ATCCG ACGTAGAACGAGGACA       TGTACCTACTGGAAGACGACCCTGTGAGG        TGCCGTCAGTCCACGC GTTTT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 005-111863094
Forward Primer: 5'-AGTACACTTGGACCCAGCTTACC-3' Tm: 59.148
Reverse Primer: 5'-TGAATCCCAGGTAGCGTACCTGC-3' Tm: 61.088
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 136, 134
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr5:111863198-111863258

Rank: 24	Homology Score: 10	RVD Match Score: 2.832	Left Half-Site RVD Score: 4.963	Right Half-Site RVD Score: 5.244
	Genomic Region: Intron	Closest Gene: SLC2A13
Match Type: R19R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TAAGA TTCAACTGACTCCTGA            GTTAGATGCAAAAAATTTT             ATAGTAGTGAGGTCTA CTGTT-3'
	3'-ATTCT AAGTTGACTGAGGACT            CAATCTACGTTTTTTAAAA             TATCATCACTCCAGAT GACAA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 012-040234817
Forward Primer: 5'-CCTTACAATTCACGGCTCTCAGAC-3' Tm: 59.038
Reverse Primer: 5'-CCACTGGAGTTGACAGATTTGGC-3' Tm: 59.148
Expected PCR Product Length: 436
Expected CelI Cleavage Product Sizes: 130, 306
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr12:40234920-40234970

Rank: 25	Homology Score: 13	RVD Match Score: 2.832	Left Half-Site RVD Score: 5.345	Right Half-Site RVD Score: 4.867
	Genomic Region: Intergenic	Closest Gene: SGCZ
Match Type: R10R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-ATGGA TGCTCCTGCCACCTGT                 GGAGCCAGCC                 TCAGGAGGCAGGTGAG GGAGG-3'
	3'-TACCT ACGAGGACGGTGGACA                 CCTCGGTCGG                 AGTCCTCCGTCCACTC CCTCC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 008-015147764
Forward Primer: 5'-CCCCACTTCATCTGCTAAACTTCC-3' Tm: 59.038
Reverse Primer: 5'-GAGGGGGAAAATGTGGGAAGTTCT-3' Tm: 59.038
Expected PCR Product Length: 313
Expected CelI Cleavage Product Sizes: 168, 145
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr8:15147909-15147950

Rank: 26	Homology Score: 10	RVD Match Score: 2.833	Left Half-Site RVD Score: 3.821	Right Half-Site RVD Score: 6.578
	Genomic Region: Intergenic	Closest Gene: MIR4686
Match Type: R25R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TCGGC TCCACCTGACTCCTAC         ACTGGCTCTGCAACCATTCCCAGCC          ACAGAGGTTGGCTGCA AGTAC-3'
	3'-AGCCG AGGTGGACTGAGGATG         TGACCGAGACGTTGGTAAGGGTCGG          TGTCTCCAACCGACGT TCATG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 011-002239013
Forward Primer: 5'-AGCTGTCCAGCCTGCAGCAT-3' Tm: 59.508
Reverse Primer: 5'-TCATATTCCTGGGTGGTCACTCC-3' Tm: 59.148
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 138, 132
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr11:2239121-2239177

Rank: 27	Homology Score: 5	RVD Match Score: 2.833	Left Half-Site RVD Score: 4.837	Right Half-Site RVD Score: 5.385
	Genomic Region: Intergenic	Closest Gene: TBX22
Match Type: R28R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TCATC TGCACATAACTACTCT        CCTTTTGAGAAACAGTTCTTGGCCTGTT        ACTGGGGTTTGGTGGA AACTG-3'
	3'-AGTAG ACGTGTATTGATGAGA        GGAAAACTCTTTGTCAAGAACCGGACAA        TGACCCCAAACCACCT TTGAC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 00X-079108050
Forward Primer: 5'-GGTGTCAGTAGCAGATAGGGATG-3' Tm: 59.148
Reverse Primer: 5'-CTGTACCCAACCCACTTTATGGC-3' Tm: 59.148
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 136, 134
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chrX:79108154-79108213

Rank: 28	Homology Score: 5	RVD Match Score: 2.835	Left Half-Site RVD Score: 5.169	Right Half-Site RVD Score: 5.058
	Genomic Region: Intron	Closest Gene: ROCK2
Match Type: R10R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-ACTAC TGCACCTAACTCACAC                 AGAAAAGAGC                 AGAGGAGGTAGGAGCA ATTTA-3'
	3'-TGATG ACGTGGATTGAGTGTG                 TCTTTTCTCG                 TCTCCTCCATCCTCGT TAAAT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Warning! Primer pair was generated using suboptimal parameters. 
Primer Index: 002-011376315
Forward Primer: 5'-CCCCTCATGGAGAACACAATACAAAAGAAAC-3' Tm: 59.958
Reverse Primer: 5'-GAGAGAAGAGTTACGCGTCTTCC-3' Tm: 59.148
Expected PCR Product Length: 307
Expected CelI Cleavage Product Sizes: 169, 138
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr2:11376461-11376502

Rank: 29	Homology Score: 13	RVD Match Score: 2.838	Left Half-Site RVD Score: 4.387	Right Half-Site RVD Score: 5.923
	Genomic Region: Intron	Closest Gene: HYDIN
Match Type: R26R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CCCTC TGCACACAACTCCTAT         GCACTCTGCACGCACCACCCTCCTGC         ACAGGCGTCCCGTGCA GGCTG-3'
	3'-GGGAG ACGTGTGTTGAGGATA         CGTGAGACGTGCGTGGTGGGAGGACG         TGTCCGCAGGGCACGT CCGAC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 016-071012605
Forward Primer: 5'-CTCCTAGCTTCCTGATTCTTTGCC-3' Tm: 59.038
Reverse Primer: 5'-CCCACCCTGGTACAGCAAATG-3' Tm: 59.378
Expected PCR Product Length: 355
Expected CelI Cleavage Product Sizes: 168, 187
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr16:71012742-71012799

Rank: 30	Homology Score: 8	RVD Match Score: 2.846	Left Half-Site RVD Score: 5.759	Right Half-Site RVD Score: 4.58
	Genomic Region: Intergenic	Closest Gene: N/A
Match Type: R20R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CTCAA TGAACTTTACTCCTCT            GAGGAAGGTTAGCAAGAGGG            ATAGGAGTCAGGAGAG GCACC-3'
	3'-GAGTT ACTTGAAATGAGGAGA            CTCCTTCCAATCGTTCTCCC            TATCCTCAGTCCTCTC CGTGG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 215-000126245
Forward Primer: 5'-GTCCCCTCTCCTTGCACTTTTC-3' Tm: 59.258
Reverse Primer: 5'-CCTTGGGAACCTTGGACAATAAGC-3' Tm: 59.038
Expected PCR Product Length: 341
Expected CelI Cleavage Product Sizes: 169, 172
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chrUn_gl000215:126386-126437

Rank: 31	Homology Score: 8	RVD Match Score: 2.846	Left Half-Site RVD Score: 5.759	Right Half-Site RVD Score: 4.58
	Genomic Region: Intergenic	Closest Gene: C21orf15
Match Type: R20R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CTCAA TGAACTTTACTCCTCT            GAGGAAGGTTAGCAAGAGGG            ATAGGAGTCAGGAGAG GCACC-3'
	3'-GAGTT ACTTGAAATGAGGAGA            CTCCTTCCAATCGTTCTCCC            TATCCTCAGTCCTCTC CGTGG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 021-015154271
Forward Primer: 5'-GTCCCCTCTCCTTGCACTTTTC-3' Tm: 59.258
Reverse Primer: 5'-CCTTGGGAACCTTGGACAATAAGC-3' Tm: 59.038
Expected PCR Product Length: 341
Expected CelI Cleavage Product Sizes: 169, 172
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr21:15154412-15154463

Rank: 32	Homology Score: 5	RVD Match Score: 2.847	Left Half-Site RVD Score: 4.565	Right Half-Site RVD Score: 5.782
	Genomic Region: Intron	Closest Gene: EXOC3L2
Match Type: R16R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-ACCTT TGCACCGAACCCCTAT              AGGGCGGGCACGAGGA              CCAGGAGATGGGTGTA TGGGG-3'
	3'-TGGAA ACGTGGCTTGGGGATA              TCCCGCCCGTGCTCCT              GGTCCTCTACCCACAT ACCCC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 019-045727578
Forward Primer: 5'-GAGAAGGGGAGTGTTCCACTG-3' Tm: 59.378
Reverse Primer: 5'-GGATAACAGAGGGCCATCAGC-3' Tm: 59.378
Expected PCR Product Length: 278
Expected CelI Cleavage Product Sizes: 148, 130
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr19:45727700-45727747

Rank: 33	Homology Score: 13	RVD Match Score: 2.85	Left Half-Site RVD Score: 5.333	Right Half-Site RVD Score: 5.005
	Genomic Region: Intergenic	Closest Gene: GAS2L3
Match Type: R29R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TTTTG CGCACCCGACTGCCGT       GGAGTGTTCCAGCAGAAGGTCGTCCACGT        ACAGGAGCTAGATGCA TCCTG-3'
	3'-AAAAC GCGTGGGCTGACGGCA       CCTCACAAGGTCGTCTTCCAGCAGGTGCA        TGTCCTCGATCTACGT AGGAC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 012-101038644
Forward Primer: 5'-CTGTGCTCCCCTTTCTGAATAGTG-3' Tm: 59.038
Reverse Primer: 5'-TACACTTGGACCCGACTTCCC-3' Tm: 59.378
Expected PCR Product Length: 272
Expected CelI Cleavage Product Sizes: 141, 131
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr12:101038753-101038813

Rank: 34	Homology Score: 2	RVD Match Score: 2.851	Left Half-Site RVD Score: 4.351	Right Half-Site RVD Score: 6.062
	Genomic Region: Intron	Closest Gene: CEP55
Match Type: R21R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-ATATA TACACCCAACTCCCAT           ATCTGGGTAATTTGGATAATT            ACAGTAGTTATCTGGA TAAAT-3'
	3'-TATAT ATGTGGGTTGAGGGTA           TAGACCCATTAAACCTATTAA            TGTCATCAATAGACCT ATTTA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 010-095287016
Forward Primer: 5'-GAGCTGCTAAAATTGCTTGCTGCC-3' Tm: 59.038
Reverse Primer: 5'-GCACCCAGCCTGTTCAGCAA-3' Tm: 59.508
Expected PCR Product Length: 335
Expected CelI Cleavage Product Sizes: 179, 156
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr10:95287167-95287219

Rank: 35	Homology Score: 13	RVD Match Score: 2.856	Left Half-Site RVD Score: 5.066	Right Half-Site RVD Score: 5.318
	Genomic Region: Intron	Closest Gene: GRIA2
Match Type: R19R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-ATCCA TACACCTGTCTGCTGT            AACCTTTTCCAGATTGGCT             ACTGGAGTGAAGTGGA CAAAA-3'
	3'-TAGGT ATGTGGACAGACGACA            TTGGAAAAGGTCTAACCGA             TGACCTCACTTCACCT GTTTT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
No Primers
Primer Index: No Primers
Forward Primer: 5'-No Primers-3' Tm: No Primers
Reverse Primer: 5'-No Primers-3' Tm: No Primers
Expected PCR Product Length: No Primers
Expected CelI Cleavage Product Sizes: No Primers, No Primers
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr4:158255134-158255184

Rank: 36	Homology Score: 8	RVD Match Score: 2.857	Left Half-Site RVD Score: 4.408	Right Half-Site RVD Score: 6.04
	Genomic Region: Intron	Closest Gene: HOMER1
Match Type: R15R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-AAGGA TACCCCTAAATCCTGT              CATTCTCCCACTTAA               CCTGGATTCAGGTGAA GACCT-3'
	3'-TTCCT ATGGGGATTTAGGACA              GTAAGAGGGTGAATT               GGACCTAAGTCCACTT CTGGA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 005-078702362
Forward Primer: 5'-GAAAGGATCCTGGATGAGGGG-3' Tm: 59.378
Reverse Primer: 5'-GTAGGAGTTTCCTGCCTCTGTC-3' Tm: 59.258
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 132, 138
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr5:78702469-78702515

Rank: 37	Homology Score: 13	RVD Match Score: 2.861	Left Half-Site RVD Score: 5.028	Right Half-Site RVD Score: 5.394
	Genomic Region: Intron	Closest Gene: C14orf166B
Match Type: R24R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TAACT TGCCCCCGACTCATGT          TTTCTTAGATTATCTCTGGCAATC          ACAGGAGGCAGAGGTA AAGCT-3'
	3'-ATTGA ACGGGGGCTGAGTACA          AAAGAATCTAATAGAGACCGTTAG          TGTCCTCCGTCTCCAT TTCGA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 014-077327606
Forward Primer: 5'-GCCTGGTATAGAACAAGCACCTG-3' Tm: 59.148
Reverse Primer: 5'-CCTCCTGTGATTGCCAGAGC-3' Tm: 59.508
Expected PCR Product Length: 368
Expected CelI Cleavage Product Sizes: 179, 189
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr14:77327755-77327810

Rank: 38	Homology Score: 10	RVD Match Score: 2.864	Left Half-Site RVD Score: 4.639	Right Half-Site RVD Score: 5.831
	Genomic Region: Intron	Closest Gene: JAKMIP3
Match Type: R16R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-AGGGG TGCACCTCACAACTGT              GGGAGCAGGTGATGTG              GCATGAGGCAGGGGCG GGGCT-3'
	3'-TCCCC ACGTGGAGTGTTGACA              CCCTCGTCCACTACAC              CGTACTCCGTCCCCGC CCCGA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 010-133959783
Forward Primer: 5'-TTTCTCTCGGCACCTCCCAC-3' Tm: 59.508
Reverse Primer: 5'-GTCCCTGCCCATGGACAAGT-3' Tm: 59.508
Expected PCR Product Length: 287
Expected CelI Cleavage Product Sizes: 157, 130
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr10:133959914-133959961

Rank: 39	Homology Score: 10	RVD Match Score: 2.867	Left Half-Site RVD Score: 5.747	Right Half-Site RVD Score: 4.74
	Genomic Region: Intergenic	Closest Gene: TNFSF15
Match Type: R20R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-ATCAC CCCAGCAGACCCCTGT            GGGTTTGCTCACATAAGGGA            ACAGGAGTTATGAGCA ATGAA-3'
	3'-TAGTG GGGTCGTCTGGGGACA            CCCAAACGAGTGTATTCCCT            TGTCCTCAATACTCGT TACTT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 009-117519299
Forward Primer: 5'-CAAAAGGACCTGCATCTAAGCACC-3' Tm: 59.038
Reverse Primer: 5'-ATTGTTCTTCCCTGGCCACCC-3' Tm: 59.378
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 135, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr9:117519406-117519457

Rank: 40	Homology Score: 10	RVD Match Score: 2.876	Left Half-Site RVD Score: 5.619	Right Half-Site RVD Score: 4.921
	Genomic Region: Intron	Closest Gene: MED27
Match Type: R27R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TTGTT TACAAATCACTCCTGC        CATCAACCTCATTTTACAGATCAGGAA         ACAGGAGTGAGCTGAA CAACG-3'
	3'-AACAA ATGTTTAGTGAGGACG        GTAGTTGGAGTAAAATGTCTAGTCCTT         TGTCCTCACTCGACTT GTTGC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 009-134936820
Forward Primer: 5'-GCTGGTAAGTGAAGTTGATGTGGC-3' Tm: 59.038
Reverse Primer: 5'-CCAGGAGCAGCTGCGATGAA-3' Tm: 59.508
Expected PCR Product Length: 318
Expected CelI Cleavage Product Sizes: 187, 131
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr9:134936976-134937034

Rank: 41	Homology Score: 5	RVD Match Score: 2.886	Left Half-Site RVD Score: 6.121	Right Half-Site RVD Score: 4.536
	Genomic Region: Intron	Closest Gene: VASH2
Match Type: R10R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GGTGG TCCACCTGCCCCTTCT                 GGTATTTTAT                 AGAGGAGTCAGATGGG AAGCT-3'
	3'-CCACC AGGTGGACGGGGAAGA                 CCATAAAATA                 TCTCCTCAGTCTACCC TTCGA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 001-213135498
Forward Primer: 5'-TGGTTTGGTCCCTTTCTTCTCCG-3' Tm: 59.148
Reverse Primer: 5'-AAGCCAGTCAATGTGCCTGGG-3' Tm: 59.378
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 135, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr1:213135610-213135651

Rank: 42	Homology Score: 5	RVD Match Score: 2.888	Left Half-Site RVD Score: 4.74	Right Half-Site RVD Score: 5.904
	Genomic Region: Intron	Closest Gene: AKD1
Match Type: R22R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TAAGT CACGCCTGACTCATGT           TCAAATGGAATGAATTACAGTA           ACATGAGTCAGGCATG TTTAC-3'
	3'-ATTCA GTGCGGACTGAGTACA           AGTTTACCTTACTTAATGTCAT           TGTACTCAGTCCGTAC AAATG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 006-109827303
Forward Primer: 5'-CTGGGGATTCGGTTTCAGCATATG-3' Tm: 59.038
Reverse Primer: 5'-CAGCAAATGGTGGGTATGGAATGAAGTC-3' Tm: 60.298
Expected PCR Product Length: 316
Expected CelI Cleavage Product Sizes: 152, 164
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr6:109827426-109827479

Rank: 43	Homology Score: 5	RVD Match Score: 2.893	Left Half-Site RVD Score: 4.948	Right Half-Site RVD Score: 5.716
	Genomic Region: Intergenic	Closest Gene: CYP26B1
Match Type: R19R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GGAGC TGCCCCTAACTCCTTC            CCCCCAAGTCCTGCCCCCA             GCAGGAGTCCTGTGGG TCACT-3'
	3'-CCTCG ACGGGGATTGAGGAAG            GGGGGTTCAGGACGGGGGT             CGTCCTCAGGACACCC AGTGA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 002-072161892
Forward Primer: 5'-GGCTGCTGTCTTTGATGATGCAAC-3' Tm: 59.038
Reverse Primer: 5'-GCCCCTGGCAATGAAGGCAA-3' Tm: 59.508
Expected PCR Product Length: 278
Expected CelI Cleavage Product Sizes: 147, 131
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr2:72162012-72162062

Rank: 44	Homology Score: 5	RVD Match Score: 2.894	Left Half-Site RVD Score: 4.116	Right Half-Site RVD Score: 6.695
	Genomic Region: Intron	Closest Gene: C7orf69
Match Type: R26R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CATCT CACACCTCACTCCTGG         CACACCCAAAGTGCAGGTAGTGAACC         AAATGAGCCAGGAGTA TTCTG-3'
	3'-GTAGA GTGTGGAGTGAGGACC         GTGTGGGTTTCACGTCCATCACTTGG         TTTACTCGGTCCTCAT AAGAC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 007-047858063
Forward Primer: 5'-CCACTTTCGGGGAAATGGTATCAC-3' Tm: 59.038
Reverse Primer: 5'-GCAAGCGTTCTATCCAAGTGCC-3' Tm: 59.258
Expected PCR Product Length: 310
Expected CelI Cleavage Product Sizes: 160, 150
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr7:47858192-47858249

Rank: 45	Homology Score: 13	RVD Match Score: 2.894	Left Half-Site RVD Score: 4.543	Right Half-Site RVD Score: 6.18
	Genomic Region: Intron	Closest Gene: CACNA2D3
Match Type: R11R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CAAGG TGCCCCACACTCCTGT                GGTCTGGAAGG                 AATGGAGCCATGTGCA CACTT-3'
	3'-GTTCC ACGGGGTGTGAGGACA                CCAGACCTTCC                 TTACCTCGGTACACGT GTGAA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 003-054731501
Forward Primer: 5'-CCTACAGTTTCCAAGGACAAGCAG-3' Tm: 59.038
Reverse Primer: 5'-CCCAGCAGTAGCTTTAGCATCC-3' Tm: 59.258
Expected PCR Product Length: 320
Expected CelI Cleavage Product Sizes: 160, 160
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr3:54731638-54731680

Rank: 46	Homology Score: 10	RVD Match Score: 2.895	Left Half-Site RVD Score: 5.374	Right Half-Site RVD Score: 5.289
	Genomic Region: Intergenic	Closest Gene: SLC16A7
Match Type: R15R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TCTCT CACATCTGCCTCCAGT              GTACCAGCAAAGATC               ACAGGGGTCAGGTCAA GGTGG-3'
	3'-AGAGA GTGTAGACGGAGGTCA              CATGGTCGTTTCTAG               TGTCCCCAGTCCAGTT CCACC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Warning! Primer pair was generated using suboptimal parameters. 
Primer Index: 012-059855477
Forward Primer: 5'-GAGAAAGGATTGAGTATTTTTCTCAGGACTTGG-3' Tm: 59.758
Reverse Primer: 5'-CAGAATTTCAGAGGCCCTCCATC-3' Tm: 59.148
Expected PCR Product Length: 404
Expected CelI Cleavage Product Sizes: 130, 274
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr12:59855582-59855628

Rank: 47	Homology Score: 5	RVD Match Score: 2.895	Left Half-Site RVD Score: 5.346	Right Half-Site RVD Score: 7.658
	Genomic Region: Intergenic	Closest Gene: SH3BP2
Match Type: R21L
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CCCTC TGCCCCTCCCTCCAGT           GCTGGGCTGTGTGGCCAGGGG      ACTGGACTGTGTGGCAGGATGA CTCCA-3'
	3'-GGGAG ACGGGGAGGGAGGTCA           CGACCCGACACACCGGTCCCC      TGACCTGACACACCGTCCTACT GAGGT-5'
	                                                               TGACGGGACACCCCGTTCCACT
	                                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 004-002790263
Forward Primer: 5'-ATGCAGAGATGGCCCAGAGC-3' Tm: 59.508
Reverse Primer: 5'-TGAGTAGGGCCAAGGCCACA-3' Tm: 59.508
Expected PCR Product Length: 375
Expected CelI Cleavage Product Sizes: 237, 138
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr4:2790469-2790527

Rank: 48	Homology Score: 10	RVD Match Score: 2.897	Left Half-Site RVD Score: 6.631	Right Half-Site RVD Score: 4.189
	Genomic Region: Intron	Closest Gene: POTEF
Match Type: R29R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-AGGAT TGCAGCCAGCTCCTGC       CCCGGTGCAGTGTATACAGTGTATACTGC        ACAGGTGTTGGGTGCA CCAAA-3'
	3'-TCCTA ACGTCGGTCGAGGACG       GGGCCACGTCACATATGTCACATATGACG        TGTCCACAACCCACGT GGTTT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Warning! Primer pair was generated using suboptimal parameters. 
Primer Index: 002-130879063
Forward Primer: 5'-AAGACGCCCACCGAGCCCA-3' Tm: 62.008
Reverse Primer: 5'-GCTAATGGGTGCAAAGTTTCTTTTAAGGAGCATA-3' Tm: 59.668
Expected PCR Product Length: 450
Expected CelI Cleavage Product Sizes: 144, 306
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr2:130879175-130879235

Rank: 49	Homology Score: 10	RVD Match Score: 2.897	Left Half-Site RVD Score: 4.189	Right Half-Site RVD Score: 6.631
	Genomic Region: Intergenic	Closest Gene: POTEE
Match Type: R29R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TTTGG TGCACCCAACACCTGT       GCAGTATACACTGTATACACTGCACCGGG        GCAGGAGCTGGCTGCA ATCCT-3'
	3'-AAACC ACGTGGGTTGTGGACA       CGTCATATGTGACATATGTGACGTGGCCC        CGTCCTCGACCGACGT TAGGA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Warning! Primer pair was generated using suboptimal parameters. 
Primer Index: 002-131974522
Forward Primer: 5'-GCTAATGGGTGCAAAGTTTCTTTTAAGGAGCATA-3' Tm: 59.668
Reverse Primer: 5'-AAGACGCCCACCGAGCCCA-3' Tm: 62.008
Expected PCR Product Length: 450
Expected CelI Cleavage Product Sizes: 309, 141
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr2:131974799-131974859

Rank: 50	Homology Score: 5	RVD Match Score: 2.904	Left Half-Site RVD Score: 5.325	Right Half-Site RVD Score: 5.406
	Genomic Region: Intron	Closest Gene: CNNM3
Match Type: R15R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GCCAC CGCACCTGGCCCCTGC              CTAGTTCTCTTGTGT               TGAGGTGTTAGGGGCA GCTGC-3'
	3'-CGGTG GCGTGGACCGGGGACG              GATCAAGAGAACACA               ACTCCACAATCCCCGT CGACG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 002-097493184
Forward Primer: 5'-TAGTAGAGGCGGGGTTTTGCCA-3' Tm: 59.258
Reverse Primer: 5'-AAAAAGGGACAGTGTCCACTGGG-3' Tm: 59.148
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 135, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr2:97493294-97493340

Rank: 51	Homology Score: 5	RVD Match Score: 2.904	Left Half-Site RVD Score: 4.264	Right Half-Site RVD Score: 6.594
	Genomic Region: Intron	Closest Gene: KIAA1199
Match Type: R16R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GCCTG CACAGCTCACTCCTGT              CCAGCCCTGGGATACC              AGGTGAGTCAGGAGAA TGACT-3'
	3'-CGGAC GTGTCGAGTGAGGACA              GGTCGGGACCCTATGG              TCCACTCAGTCCTCTT ACTGA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 015-081145141
Forward Primer: 5'-GCGTTAAGTGTCTGGAAGGCG-3' Tm: 59.378
Reverse Primer: 5'-GGAACTCTCAGCCACAACAAACAC-3' Tm: 59.038
Expected PCR Product Length: 339
Expected CelI Cleavage Product Sizes: 198, 141
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr15:81145313-81145360

Rank: 52	Homology Score: 2	RVD Match Score: 2.905	Left Half-Site RVD Score: 5.281	Right Half-Site RVD Score: 5.456
	Genomic Region: Intron	Closest Gene: LGMN
Match Type: R12R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TCTGT CCCCCCATACTCCTGT                GTGGCAGGGAGC                ATAGGAGGCAGGGCCG TGCTC-3'
	3'-AGACA GGGGGGTATGAGGACA                CACCGTCCCTCG                TATCCTCCGTCCCGGC ACGAG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 014-093175798
Forward Primer: 5'-CTCCCTACTACCGAAGAGGTC-3' Tm: 59.378
Reverse Primer: 5'-GATCTGGAGGAGTCCAGGCA-3' Tm: 59.508
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 131, 139
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr14:93175905-93175948

Rank: 53	Homology Score: 8	RVD Match Score: 2.909	Left Half-Site RVD Score: 6.147	Right Half-Site RVD Score: 4.67
	Genomic Region: Intron	Closest Gene: MYBPHL
Match Type: R30R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-ATGTT TTCACATGCTTCCTGT       GATACTCAGTCCCAGAGCTCTCTGTAAGGA       AGAGGAGTTAGGAGGA AGAAA-3'
	3'-TACAA AAGTGTACGAAGGACA       CTATGAGTCAGGGTCTCGAGAGACATTCCT       TCTCCTCAATCCTCCT TCTTT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 001-109847447
Forward Primer: 5'-GCAGGCTTGCCAAACTAAAAGCTC-3' Tm: 59.038
Reverse Primer: 5'-TCAGAGAGCCAACAGAGCCC-3' Tm: 59.508
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 133, 137
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr1:109847547-109847608

Rank: 54	Homology Score: 10	RVD Match Score: 2.91	Left Half-Site RVD Score: 6.642	Right Half-Site RVD Score: 4.262
	Genomic Region: Intergenic	Closest Gene: NDST3
Match Type: R26R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TTACA TGCATCTATCTCATTT         AATGCTTTTAACAATGCTACAACTAA         AAAGTAGTCAGGTGTA GACTG-3'
	3'-AATGT ACGTAGATAGAGTAAA         TTACGAAAATTGTTACGATGTTGATT         TTTCATCAGTCCACAT CTGAC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Warning! Primer pair was generated using suboptimal parameters. 
Primer Index: 004-118702135
Forward Primer: 5'-GCAGGGACACTGGTGTTATGTCT-3' Tm: 59.148
Reverse Primer: 5'-GATAGTAACTTATTTAGAATTGAGGTTGTGTGATGGAG-3' Tm: 59.358
Expected PCR Product Length: 429
Expected CelI Cleavage Product Sizes: 293, 136
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr4:118702397-118702454

Rank: 55	Homology Score: 5	RVD Match Score: 2.911	Left Half-Site RVD Score: 4.705	Right Half-Site RVD Score: 6.128
	Genomic Region: Intron	Closest Gene: FHIT
Match Type: R20R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CTGCT TACACCTGTCTCCTAA            TAAAATCAACTGCCAATCAC            AGGGGAGGCTGGGGCA TTTTA-3'
	3'-GACGA ATGTGGACAGAGGATT            ATTTTAGTTGACGGTTAGTG            TCCCCTCCGACCCCGT AAAAT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 003-060011649
Forward Primer: 5'-GTACTGCCAGAGTGTCAAGAAAGC-3' Tm: 59.038
Reverse Primer: 5'-TAGGAAGAGGACAGTAGGCTAGG-3' Tm: 59.148
Expected PCR Product Length: 286
Expected CelI Cleavage Product Sizes: 156, 130
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr3:60011777-60011828

Rank: 56	Homology Score: 8	RVD Match Score: 2.913	Left Half-Site RVD Score: 4.624	Right Half-Site RVD Score: 6.232
	Genomic Region: Intergenic	Closest Gene: PISRT1
Match Type: R11R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GTGAT TACCCCTGACTCCACT                CCTGGATGAGG                 TCAGGGGTCATCTGCA CAGAC-3'
	3'-CACTA ATGGGGACTGAGGTGA                GGACCTACTCC                 AGTCCCCAGTAGACGT GTCTG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 003-138953648
Forward Primer: 5'-CTTGGTGTCTGAGTGGCAAAACTG-3' Tm: 59.038
Reverse Primer: 5'-TATGGGCCTGTTTTACACGGGCCT-3' Tm: 60.908
Expected PCR Product Length: 281
Expected CelI Cleavage Product Sizes: 148, 133
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr3:138953773-138953815

Rank: 57	Homology Score: 5	RVD Match Score: 2.913	Left Half-Site RVD Score: 4.72	Right Half-Site RVD Score: 6.128
	Genomic Region: Intergenic	Closest Gene: TBC1D22A
Match Type: R17R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TGAGG CAAACCTCACTCCAGT             TTCTGGGGCAGCCAGAC              ACAGGGGACTGGGGCA GGAGC-3'
	3'-ACTCC GTTTGGAGTGAGGTCA             AAGACCCCGTCGGTCTG              TGTCCCCTGACCCCGT CCTCG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 022-047592728
Forward Primer: 5'-AATGAGGCCTGGGAGCAGGA-3' Tm: 59.508
Reverse Primer: 5'-CACCAAGAGCACCCCAAAGG-3' Tm: 59.508
Expected PCR Product Length: 274
Expected CelI Cleavage Product Sizes: 133, 141
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr22:47592835-47592883

Rank: 58	Homology Score: 2	RVD Match Score: 2.915	Left Half-Site RVD Score: 5.787	Right Half-Site RVD Score: 5.038
	Genomic Region: Intron	Closest Gene: FAM19A2
Match Type: R10R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CTATG CATACCTGTCACCTGT                 CCATGAGGAG                 AGAGGTGACAGGTGTG CATAG-3'
	3'-GATAC GTATGGACAGTGGACA                 GGTACTCCTC                 TCTCCACTGTCCACAC GTATC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 012-062445219
Forward Primer: 5'-GAGAGGATACAAGGTCAGGTATGG-3' Tm: 59.038
Reverse Primer: 5'-GGCCCATTTTAGAAAACATTTGCTGACTCC-3' Tm: 60.058
Expected PCR Product Length: 446
Expected CelI Cleavage Product Sizes: 271, 175
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr12:62445467-62445508

Rank: 59	Homology Score: 10	RVD Match Score: 2.918	Left Half-Site RVD Score: 5.228	Right Half-Site RVD Score: 5.612
	Genomic Region: Intergenic	Closest Gene: DHRS2
Match Type: R10R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TCTCC TTCACCTGACTCTAGT                 TGTTGCTGGA                 ACAGGACTTTGTTGTA TATTC-3'
	3'-AGAGG AAGTGGACTGAGATCA                 ACAACGACCT                 TGTCCTGAAACAACAT ATAAG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 014-024242484
Forward Primer: 5'-CAAGTCTTCCTCAGCCATCAGACA-3' Tm: 59.038
Reverse Primer: 5'-CCAGACACATCAACAGAGTGGAAG-3' Tm: 59.038
Expected PCR Product Length: 298
Expected CelI Cleavage Product Sizes: 138, 160
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr14:24242599-24242640

Rank: 60	Homology Score: 5	RVD Match Score: 2.919	Left Half-Site RVD Score: 5.467	Right Half-Site RVD Score: 5.377
	Genomic Region: Exon	Closest Gene: LELP1
Match Type: R24R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TAACA TCCACCTCACTCAACT          GCTTGTCAAGTTCACCACCAACAC          AGAGGGGTAAGTTGCA TTTGG-3'
	3'-ATTGT AGGTGGAGTGAGTTGA          CGAACAGTTCAAGTGGTGGTTGTG          TCTCCCCATTCAACGT AAACC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 001-153175860
Forward Primer: 5'-CTCATAAGTGGAGGTCATTTCCCC-3' Tm: 59.038
Reverse Primer: 5'-GCACACACCAGTTCTTACCACTGA-3' Tm: 59.038
Expected PCR Product Length: 300
Expected CelI Cleavage Product Sizes: 132, 168
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr1:153175962-153176017

Rank: 61	Homology Score: 5	RVD Match Score: 2.923	Left Half-Site RVD Score: 5.84	Right Half-Site RVD Score: 5.046
	Genomic Region: Intergenic	Closest Gene: TMEM185A
Match Type: R27R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TTTCA TTCACCTCATTCATAT        CATCCCCACCAAAAGTATAGCCATTTC         AGTGGAGTCAGCTGTA CACCA-3'
	3'-AAAGT AAGTGGAGTAAGTATA        GTAGGGGTGGTTTTCATATCGGTAAAG         TCACCTCAGTCGACAT GTGGT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 00X-148727617
Forward Primer: 5'-GAGTCTTCCATAGCTGAGTGCAAC-3' Tm: 59.038
Reverse Primer: 5'-ACCCAACATCTGCCATCTGCAAG-3' Tm: 59.148
Expected PCR Product Length: 271
Expected CelI Cleavage Product Sizes: 140, 131
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chrX:148727726-148727784

Rank: 62	Homology Score: 2	RVD Match Score: 2.924	Left Half-Site RVD Score: 5.753	Right Half-Site RVD Score: 5.134
	Genomic Region: Intergenic	Closest Gene: ODZ2
Match Type: R18R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TTTAT TAAACCGCACTCCTTT             GGTGCTTCCTCTGGTGGC             AGAGGAGTGGGGTTTA ATAAA-3'
	3'-AAATA ATTTGGCGTGAGGAAA             CCACGAAGGAGACCACCG             TCTCCTCACCCCAAAT TATTT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 005-166596052
Forward Primer: 5'-CGTTGCCCAGCTTGATGCCT-3' Tm: 59.508
Reverse Primer: 5'-GGAAGTTAGGCCCTAGGACC-3' Tm: 59.508
Expected PCR Product Length: 388
Expected CelI Cleavage Product Sizes: 132, 256
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr5:166596157-166596206

Rank: 63	Homology Score: 2	RVD Match Score: 2.924	Left Half-Site RVD Score: 6.608	Right Half-Site RVD Score: 4.387
	Genomic Region: Exon	Closest Gene: GOSR2
Match Type: R21R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GCTGA TGCAAGAAACTCCGGT           AGCCAGCCTCTGTGACCTTGT            ACATGAGTTTGGTGTG CCTAT-3'
	3'-CGACT ACGTTCTTTGAGGCCA           TCGGTCGGAGACACTGGAACA            TGTACTCAAACCACAC GGATA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 017-045018304
Forward Primer: 5'-TAACAGTGTGACTCTCTCCACCG-3' Tm: 59.148
Reverse Primer: 5'-GTACAGAACTCCGCAGTGAAGTC-3' Tm: 59.148
Expected PCR Product Length: 316
Expected CelI Cleavage Product Sizes: 130, 186
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr17:45018406-45018458

Rank: 64	Homology Score: 10	RVD Match Score: 2.925	Left Half-Site RVD Score: 5.776	Right Half-Site RVD Score: 5.124
	Genomic Region: Exon	Closest Gene: KIF20A
Match Type: R14R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CATCT TACACCCCTCTCCTTT               GGCCCCAGATCTCA               ACTGGATTCATGTGCA AGATG-3'
	3'-GTAGA ATGTGGGGAGAGGAAA               CCGGGGTCTAGAGT               TGACCTAAGTACACGT TCTAC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 005-137519020
Forward Primer: 5'-GCGAGGATCAAAATGGCAATCCC-3' Tm: 59.148
Reverse Primer: 5'-GGCAGAGGACTAATGTTTCCTGG-3' Tm: 59.148
Expected PCR Product Length: 310
Expected CelI Cleavage Product Sizes: 140, 170
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr5:137519135-137519180

Rank: 65	Homology Score: 10	RVD Match Score: 2.926	Left Half-Site RVD Score: 4.264	Right Half-Site RVD Score: 6.778
	Genomic Region: Intron	Closest Gene: TGFBR2
Match Type: R17R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-ATTCC TCCACCTGAGTCCTAT             TGAGGTGCTCCCCATTA              TAAGGAGTCCTGTGAA TCAAA-3'
	3'-TAAGG AGGTGGACTCAGGATA             ACTCCACGAGGGGTAAT              ATTCCTCAGGACACTT AGTTT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 003-030699112
Forward Primer: 5'-CTGGCACAGAGTGAGCACTTAATG-3' Tm: 59.038
Reverse Primer: 5'-GGTCGTCATGAGGAGAACACCTT-3' Tm: 59.148
Expected PCR Product Length: 327
Expected CelI Cleavage Product Sizes: 172, 155
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr3:30699258-30699306

Rank: 66	Homology Score: 8	RVD Match Score: 2.932	Left Half-Site RVD Score: 5.739	Right Half-Site RVD Score: 5.205
	Genomic Region: Intergenic	Closest Gene: TEAD1
Match Type: R20R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-ATAAC TCCTCCCGACCCCTGT            CCTCAGCATCACCATGGGGT            TCATGATTCAGGTGTA TGCAG-3'
	3'-TATTG AGGAGGGCTGGGGACA            GGAGTCGTAGTGGTACCCCA            AGTACTAAGTCCACAT ACGTC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 011-012966658
Forward Primer: 5'-TGAGGGATAGAGCAGAGGATGG-3' Tm: 59.258
Reverse Primer: 5'-GGGGGAATGATGTCACTGGAAC-3' Tm: 59.258
Expected PCR Product Length: 295
Expected CelI Cleavage Product Sizes: 131, 164
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr11:12966761-12966812

Rank: 67	Homology Score: 2	RVD Match Score: 2.932	Left Half-Site RVD Score: 4.936	Right Half-Site RVD Score: 6.031
	Genomic Region: Intergenic	Closest Gene: SYNE3
Match Type: R23R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CTTCT CCCACCTCACCCCTGA          GGACCAGCAGAGTCTGATTTGGG           ACAAGGGTCAGGGGAG GGACA-3'
	3'-GAAGA GGGTGGAGTGGGGACT          CCTGGTCGTCTCAGACTAAACCC           TGTTCCCAGTCCCCTC CCTGT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 014-095952113
Forward Primer: 5'-GCCTGGATCCCACTGGACTA-3' Tm: 59.508
Reverse Primer: 5'-ACAGCCCTTCTCCCTTCTGC-3' Tm: 59.508
Expected PCR Product Length: 275
Expected CelI Cleavage Product Sizes: 144, 131
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr14:95952228-95952282

Rank: 68	Homology Score: 10	RVD Match Score: 2.935	Left Half-Site RVD Score: 5.124	Right Half-Site RVD Score: 5.849
	Genomic Region: Intron	Closest Gene: USP43
Match Type: R21R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TCATG TGAAACTGACTCCTGA           GAAAATGTGGGCAGGAATGAT            TTAGCAGGCAGGTGGA AGGTG-3'
	3'-AGTAC ACTTTGACTGAGGACT           CTTTTACACCCGTCCTTACTA            AATCGTCCGTCCACCT TCCAC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 017-009569404
Forward Primer: 5'-GGGAATGAAAAGGACAGCAGGAC-3' Tm: 59.148
Reverse Primer: 5'-CGGCCCTGCGCGTTATTTCT-3' Tm: 59.508
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 135, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr17:9569507-9569559

Rank: 69	Homology Score: 5	RVD Match Score: 2.935	Left Half-Site RVD Score: 5.678	Right Half-Site RVD Score: 5.286
	Genomic Region: Intergenic	Closest Gene: AKIRIN2
Match Type: R25R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CTGTG TGCACAGAACTCCTGG         GGGGGAGATGGAATAAGGAAAGCCA          ATGTGATTCAGGTGTA GTGAG-3'
	3'-GACAC ACGTGTCTTGAGGACC         CCCCCTCTACCTTATTCCTTTCGGT          TACACTAAGTCCACAT CACTC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 006-088451104
Forward Primer: 5'-GATCTCCTTGAAGGCACATGCAG-3' Tm: 59.148
Reverse Primer: 5'-GGCCTGGGAAACATAATGACACC-3' Tm: 59.148
Expected PCR Product Length: 325
Expected CelI Cleavage Product Sizes: 130, 195
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr6:88451204-88451260

Rank: 70	Homology Score: 2	RVD Match Score: 2.938	Left Half-Site RVD Score: 6.121	Right Half-Site RVD Score: 4.898
	Genomic Region: Intron	Closest Gene: PTK2B
Match Type: R16R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GAGAA TCCCCCTGATTCCCCT              GTAAAGGCAAGCTGAG              CCAGGAGTGGGGTGTG GATGA-3'
	3'-CTCTT AGGGGGACTAAGGGGA              CATTTCCGTTCGACTC              GGTCCTCACCCCACAC CTACT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 008-027202946
Forward Primer: 5'-ATGTTGACATCTGGGCCACAGC-3' Tm: 59.258
Reverse Primer: 5'-CCTCTCATCTCCTCCTTTGATCAG-3' Tm: 59.038
Expected PCR Product Length: 288
Expected CelI Cleavage Product Sizes: 131, 157
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr8:27203051-27203098

Rank: 71	Homology Score: 2	RVD Match Score: 2.94	Left Half-Site RVD Score: 5.972	Right Half-Site RVD Score: 5.046
	Genomic Region: Intergenic	Closest Gene: TMEM18
Match Type: R24R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CCGGG CCAGCATGACTCCTGT          GAGCCTCGGCCTCCACACTGACAA          ACAGGAGTGTGGTCTG AGACA-3'
	3'-GGCCC GGTCGTACTGAGGACA          CTCGGAGCCGGAGGTGTGACTGTT          TGTCCTCACACCAGAC TCTGT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 002-000581727
Forward Primer: 5'-CTCGGGCCATTGATTTGGCAAG-3' Tm: 59.258
Reverse Primer: 5'-CCCCAGGGCACCTTTATGTC-3' Tm: 59.508
Expected PCR Product Length: 335
Expected CelI Cleavage Product Sizes: 141, 194
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr2:581838-581893

Rank: 72	Homology Score: 5	RVD Match Score: 2.941	Left Half-Site RVD Score: 4.499	Right Half-Site RVD Score: 6.606
	Genomic Region: Intergenic	Closest Gene: CEBPB
Match Type: R14R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TTGTC CCCACCTTCCTCCTGT               GGCTCCCACACAGG               ACAGAGGTGAGGGTCA TCTGC-3'
	3'-AACAG GGGTGGAAGGAGGACA               CCGAGGGTGTGTCC               TGTCTCCACTCCCAGT AGACG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 020-048858983
Forward Primer: 5'-TACTAGATGCTCAATACCTGGCGG-3' Tm: 59.038
Reverse Primer: 5'-CTTGGGAAGCCACCTGGTCA-3' Tm: 59.508
Expected PCR Product Length: 268
Expected CelI Cleavage Product Sizes: 137, 131
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr20:48859095-48859140

Rank: 73	Homology Score: 8	RVD Match Score: 2.942	Left Half-Site RVD Score: 6.157	Right Half-Site RVD Score: 4.892
	Genomic Region: Intron	Closest Gene: RPL18A
Match Type: R30R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TAGGG TGGCCCTGCCTCCTGC       TTCCCGAATCTGTGGTCACTAGATGTAGGA       AAGGGAGTGAGGTGGA TCTCC-3'
	3'-ATCCC ACCGGGACGGAGGACG       AAGGGCTTAGACACCAGTGATCTACATCCT       TTCCCTCACTCCACCT AGAGG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 019-017971876
Forward Primer: 5'-CTCAATTAATCCTCCCTGGAGACC-3' Tm: 59.038
Reverse Primer: 5'-ATTCGCATGCGGTAGAGGGG-3' Tm: 59.508
Expected PCR Product Length: 303
Expected CelI Cleavage Product Sizes: 143, 160
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr19:17971986-17972047

Rank: 74	Homology Score: 2	RVD Match Score: 2.944	Left Half-Site RVD Score: 5.848	Right Half-Site RVD Score: 5.19
	Genomic Region: Intergenic	Closest Gene: FBXO33
Match Type: R16R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GCCAC CACACCTGGCAGCTGT              CTTATTTAAAAATAAA              ATGGGAGGCAGGTTTA CCTGA-3'
	3'-CGGTG GTGTGGACCGTCGACA              GAATAAATTTTTATTT              TACCCTCCGTCCAAAT GGACT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 014-040804929
Forward Primer: 5'-CCACCACGCCTGGCTAATTTTTC-3' Tm: 59.148
Reverse Primer: 5'-GCCCACCCATCAGAGAGTTTC-3' Tm: 59.378
Expected PCR Product Length: 335
Expected CelI Cleavage Product Sizes: 159, 176
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr14:40805062-40805109

Rank: 75	Homology Score: 5	RVD Match Score: 2.945	Left Half-Site RVD Score: 4.652	Right Half-Site RVD Score: 6.456
	Genomic Region: Intergenic	Closest Gene: CNR2
Match Type: R19R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CAGCC TACATCAGACTCCTAT            CTGGGCTGGAATTGGAGAC             CCAGGGGTGAGCTGGA AGAGA-3'
	3'-GTCGG ATGTAGTCTGAGGATA            GACCCGACCTTAACCTCTG             GGTCCCCACTCGACCT TCTCT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 001-024242432
Forward Primer: 5'-TCCCAAGAGCAAGACATTGGCAG-3' Tm: 59.148
Reverse Primer: 5'-GCAATGGAGGACCAGGCGTT-3' Tm: 59.508
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 135, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr1:24242540-24242590

Rank: 76	Homology Score: 5	RVD Match Score: 2.947	Left Half-Site RVD Score: 5.002	Right Half-Site RVD Score: 6.076
	Genomic Region: Intron	Closest Gene: ULK4
Match Type: R13R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CTACC CCCACCTTAGTCCTAT               TTATTCAGAGAGC                AGAGGAATCAGGAGAA GGGAG-3'
	3'-GATGG GGGTGGAATCAGGATA               AATAAGTCTCTCG                TCTCCTTAGTCCTCTT CCCTC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 003-041668393
Forward Primer: 5'-CTGGCACAGCTGTATTTCATGTCC-3' Tm: 59.038
Reverse Primer: 5'-GTGATCTCCATGGACCTGTCCT-3' Tm: 59.258
Expected PCR Product Length: 434
Expected CelI Cleavage Product Sizes: 303, 131
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr3:41668672-41668716

Rank: 77	Homology Score: 5	RVD Match Score: 2.949	Left Half-Site RVD Score: 6.911	Right Half-Site RVD Score: 4.306
	Genomic Region: Intergenic	Closest Gene: DZIP1L
Match Type: R23R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CTGAG TTCTCCTGGCTCCTCC          AGCTTCCTTCTCTCTTTCTGTTC           ACAGTGGTCAGGTGTG CATTG-3'
	3'-GACTC AAGAGGACCGAGGAGG          TCGAAGGAAGAGAGAAAGACAAG           TGTCACCAGTCCACAC GTAAC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 003-137780481
Forward Primer: 5'-AGAGCCTGGCCATTTCTGCAC-3' Tm: 59.378
Reverse Primer: 5'-TAGGTCTTTTGGGACATGGTTGCC-3' Tm: 59.038
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 135, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr3:137780587-137780641

Rank: 78	Homology Score: 5	RVD Match Score: 2.949	Left Half-Site RVD Score: 5.669	Right Half-Site RVD Score: 5.399
	Genomic Region: Intron	Closest Gene: C3orf67
Match Type: R27R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TTTTC TGTCCCTAACTCCTGG        ATTTGACACTGCTTTGTCACAATTTAG         ATGAGAGTGAGGTGGA GCCTT-3'
	3'-AAAAG ACAGGGATTGAGGACC        TAAACTGTGACGAAACAGTGTTAAATC         TACTCTCACTCCACCT CGGAA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 003-058824763
Forward Primer: 5'-GCCAACTGATATATCTGGAGTGGG-3' Tm: 59.038
Reverse Primer: 5'-TGCCAGGCCTTCCACAAGGT-3' Tm: 59.508
Expected PCR Product Length: 404
Expected CelI Cleavage Product Sizes: 273, 131
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr3:58825005-58825063

Rank: 79	Homology Score: 10	RVD Match Score: 2.95	Left Half-Site RVD Score: 5.662	Right Half-Site RVD Score: 5.414
	Genomic Region: Intergenic	Closest Gene: GHITM
Match Type: R29R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TAAGC TGCATCTTGCTCCTGT       ATGTGGACAAACTTCTGCTGGGACACTCC        ACGGCAGTCGGGTGTG CAAAA-3'
	3'-ATTCG ACGTAGAACGAGGACA       TACACCTGTTTGAAGACGACCCTGTGAGG        TGCCGTCAGCCCACAC GTTTT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 010-085871707
Forward Primer: 5'-AGTACACTTGGACCCGGCTTC-3' Tm: 59.378
Reverse Primer: 5'-TGAATCCCAGGTAGCGTACCTGC-3' Tm: 61.088
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 136, 134
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr10:85871811-85871871

Rank: 80	Homology Score: 10	RVD Match Score: 2.955	Left Half-Site RVD Score: 4.965	Right Half-Site RVD Score: 6.177
	Genomic Region: Intron	Closest Gene: EIF2B3
Match Type: R12R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-AAAAA TGCAGCTCACACCTGT                GATCCCAGTACT                TTAGGAGGCAGAGGCA GGCAG-3'
	3'-TTTTT ACGTCGAGTGTGGACA                CTAGGGTCATGA                AATCCTCCGTCTCCGT CCGTC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 001-045422945
Forward Primer: 5'-GCCCCCAAACACCACAAAGTC-3' Tm: 59.378
Reverse Primer: 5'-ATTACAGGCACATGCCACCACG-3' Tm: 59.258
Expected PCR Product Length: 370
Expected CelI Cleavage Product Sizes: 240, 130
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr1:45423161-45423204

Rank: 81	Homology Score: 13	RVD Match Score: 2.955	Left Half-Site RVD Score: 4.992	Right Half-Site RVD Score: 6.149
	Genomic Region: Intergenic	Closest Gene: FRG1
Match Type: R14R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GTTTC TACCCCTGACCCCTGC               AGTCTACTCTGAGC               ACAGCAGTCAGAAGCA TTCTT-3'
	3'-CAAAG ATGGGGACTGGGGACG               TCAGATGAGACTCG               TGTCGTCAGTCTTCGT AAGAA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 004-190659833
Forward Primer: 5'-CTGCCTCCAATCCCTCAGAAAATG-3' Tm: 59.038
Reverse Primer: 5'-CTGGTATTGGGTCCAGACCAC-3' Tm: 59.378
Expected PCR Product Length: 308
Expected CelI Cleavage Product Sizes: 178, 130
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr4:190659986-190660031

Rank: 82	Homology Score: 2	RVD Match Score: 2.956	Left Half-Site RVD Score: 6.991	Right Half-Site RVD Score: 4.291
	Genomic Region: Exon	Closest Gene: LOC400752
Match Type: R30R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CAGGG TTCAGCAGCATCCTGT       TCATCATGAGCAGAGGGGTGACTGAATAGA       ATGGGAGGCAGGTGTG GCCTG-3'
	3'-GTCCC AAGTCGTCGTAGGACA       AGTAGTACTCGTCTCCCCACTGACTTATCT       TACCCTCCGTCCACAC CGGAC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 001-045770634
Forward Primer: 5'-AATGGGGACGCTTCCGACTC-3' Tm: 59.508
Reverse Primer: 5'-TTGTGGGGCCCCAAGATTTCC-3' Tm: 59.378
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 138, 132
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr1:45770739-45770800

Rank: 83	Homology Score: 2	RVD Match Score: 2.956	Left Half-Site RVD Score: 4.941	Right Half-Site RVD Score: 6.217
	Genomic Region: Intergenic	Closest Gene: ANKRD7
Match Type: R22R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TCAAT CACACCTCATTCCTTT           CTCATCATATCAGAACAAACAA           AGAGAAGTGTTGTGCA TCTTT-3'
	3'-AGTTA GTGTGGAGTAAGGAAA           GAGTAGTATAGTCTTGTTTGTT           TCTCTTCACAACACGT AGAAA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 007-118742205
Forward Primer: 5'-GACAACCTGGTGAAGGCAACAGA-3' Tm: 59.148
Reverse Primer: 5'-GTGCAGACATCCAGGATAAAACCC-3' Tm: 59.038
Expected PCR Product Length: 444
Expected CelI Cleavage Product Sizes: 280, 164
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr7:118742456-118742509

Rank: 84	Homology Score: 2	RVD Match Score: 2.956	Left Half-Site RVD Score: 6.608	Right Half-Site RVD Score: 4.603
	Genomic Region: Intron	Closest Gene: DNAI2
Match Type: R16R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CGCCA TAAAGCTGACGCCAGT              GCCTGTAGGGGCCTGG              ACAGGGGTTGGGTGGG TTGGG-3'
	3'-GCGGT ATTTCGACTGCGGTCA              CGGACATCCCCGGACC              TGTCCCCAACCCACCC AACCC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 017-072308236
Forward Primer: 5'-AGGAGCAGACCGATGAGGAG-3' Tm: 59.508
Reverse Primer: 5'-GGGATGCTTTGGAAGGAAGGG-3' Tm: 59.378
Expected PCR Product Length: 311
Expected CelI Cleavage Product Sizes: 140, 171
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr17:72308350-72308397

Rank: 85	Homology Score: 2	RVD Match Score: 2.957	Left Half-Site RVD Score: 4.985	Right Half-Site RVD Score: 6.173
	Genomic Region: Intron	Closest Gene: IGSF21
Match Type: R26R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GGTGG CCCATCTGACTCCCAT         CAGGCGCTCAGTGAAGGGTCAGGAAG         AGGGGAGGCAGGAGGA GGAGG-3'
	3'-CCACC GGGTAGACTGAGGGTA         GTCCGCGAGTCACTTCCCAGTCCTTC         TCCCCTCCGTCCTCCT CCTCC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 001-018569392
Forward Primer: 5'-TCATGTGACCCGCACCCTTG-3' Tm: 59.508
Reverse Primer: 5'-GGGTCCAGAGCTTCCTAAAATCG-3' Tm: 59.148
Expected PCR Product Length: 294
Expected CelI Cleavage Product Sizes: 131, 163
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr1:18569492-18569549

Rank: 86	Homology Score: 2	RVD Match Score: 2.958	Left Half-Site RVD Score: 5.038	Right Half-Site RVD Score: 6.121
	Genomic Region: Intergenic	Closest Gene: LOC100287010
Match Type: R12R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-AGTTG TCCACCAACCTCCTCT                GGTCAGCAATTA                AGAGGAGGCTGGTAGA CAACC-3'
	3'-TCAAC AGGTGGTTGGAGGAGA                CCAGTCGTTAAT                TCTCCTCCGACCATCT GTTGG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 002-104369295
Forward Primer: 5'-AGGAAGGGAAACTTCACCTCCTG-3' Tm: 59.148
Reverse Primer: 5'-CCACCACCACAGTCAAGATGC-3' Tm: 59.378
Expected PCR Product Length: 360
Expected CelI Cleavage Product Sizes: 131, 229
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr2:104369402-104369445

Rank: 87	Homology Score: 10	RVD Match Score: 2.958	Left Half-Site RVD Score: 6.444	Right Half-Site RVD Score: 4.756
	Genomic Region: Intron	Closest Gene: PLCG1
Match Type: R20R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-AGCTT CTCTCCAGACTCTTGT            GTGTGTGTGTACACAGATGC            ACAGGATTCAGTTGGA GTTTA-3'
	3'-TCGAA GAGAGGTCTGAGAACA            CACACACACATGTGTCTACG            TGTCCTAAGTCAACCT CAAAT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 020-039770399
Forward Primer: 5'-CCAGCTGGGATCCTCTGTTTC-3' Tm: 59.378
Reverse Primer: 5'-CCTAGCACTGGGCAAATGGG-3' Tm: 59.508
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 135, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr20:39770506-39770557

Rank: 88	Homology Score: 17	RVD Match Score: 2.96	Left Half-Site RVD Score: 4.122	Right Half-Site RVD Score: 7.246
	Genomic Region: Intergenic	Closest Gene: SH3BP4
Match Type: R23R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GCAAT TGCACCTCACTTCTGT          GGAGTGCTTCCTGTGTATCAGGC           ACAGAAGCCCGATGTA TTCTC-3'
	3'-CGTTA ACGTGGAGTGAAGACA          CCTCACGAAGGACACATAGTCCG           TGTCTTCGGGCTACAT AAGAG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 002-235805535
Forward Primer: 5'-ACCTCCACCAGACCACGAATG-3' Tm: 59.378
Reverse Primer: 5'-GACCCTTAGCAGGTCACACTC-3' Tm: 59.378
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 135, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr2:235805641-235805695

Rank: 89	Homology Score: 2	RVD Match Score: 2.96	Left Half-Site RVD Score: 5.274	Right Half-Site RVD Score: 5.884
	Genomic Region: Intron	Closest Gene: DOCK3
Match Type: R27R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CCCCA CCCACCTCACTCTTTT        TGATGTTGGAAAAATAACTGGCTAGGT         ATAGGGATCAGTTGGA GGAGT-3'
	3'-GGGGT GGGTGGAGTGAGAAAA        ACTACAACCTTTTTATTGACCGATCCA         TATCCCTAGTCAACCT CCTCA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Warning! Primer pair was generated using suboptimal parameters. 
Primer Index: 003-051035046
Forward Primer: 5'-CTTCTTAGAAATGGGACTCAGAAAGGACC-3' Tm: 60.178
Reverse Primer: 5'-CAATTGAGAGAAGCAGCAAAGTCTTCTACAG-3' Tm: 59.958
Expected PCR Product Length: 327
Expected CelI Cleavage Product Sizes: 138, 189
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr3:51035153-51035211

Rank: 90	Homology Score: 10	RVD Match Score: 2.961	Left Half-Site RVD Score: 4.174	Right Half-Site RVD Score: 7.182
	Genomic Region: Intergenic	Closest Gene: KCNJ2
Match Type: R26R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GCCAC CGCACCTGGCTCCTCT         GGCTATTTTTGCCAGGATTTTAAACT         AAAAGTGTCAGGAGAA TGCTT-3'
	3'-CGGTG GCGTGGACCGAGGAGA         CCGATAAAAACGGTCCTAAAATTTGA         TTTTCACAGTCCTCTT ACGAA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 017-068378451
Forward Primer: 5'-TGGGCTTCCTCCATGTTGGTC-3' Tm: 59.378
Reverse Primer: 5'-ATGACAGGACCTGGCTCACC-3' Tm: 59.508
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 132, 138
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr17:68378552-68378609

Rank: 91	Homology Score: 5	RVD Match Score: 2.961	Left Half-Site RVD Score: 5.161	Right Half-Site RVD Score: 6.01
	Genomic Region: Intron	Closest Gene: PARD3
Match Type: R10R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-ATCCA CAAACCTTAATCCTGT                 ATGAGTGCGG                 TAAGGTGTCAGATGCA ATGGC-3'
	3'-TAGGT GTTTGGAATTAGGACA                 TACTCACGCC                 ATTCCACAGTCTACGT TACCG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 010-034494294
Forward Primer: 5'-GCTAGATAGGCCGGGTGAAATG-3' Tm: 59.258
Reverse Primer: 5'-AGCTCCCAGGCTTTCACCAC-3' Tm: 59.508
Expected PCR Product Length: 302
Expected CelI Cleavage Product Sizes: 172, 130
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr10:34494443-34494484

Rank: 92	Homology Score: 2	RVD Match Score: 2.962	Left Half-Site RVD Score: 5.371	Right Half-Site RVD Score: 5.795
	Genomic Region: Intron	Closest Gene: TNNT1
Match Type: R20R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TCCTC CCCACCTGTCTCCCCT            TTCACCTTCCCATACATCAC            GTAGGGGGCAGGGGCA TAAAG-3'
	3'-AGGAG GGGTGGACAGAGGGGA            AAGTGGAAGGGTATGTAGTG            CATCCCCCGTCCCCGT ATTTC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 019-055659570
Forward Primer: 5'-ACCGAGGACATTTCCTAAGAAGGG-3' Tm: 59.038
Reverse Primer: 5'-GTGGGTATTGGGATTTGGGGC-3' Tm: 59.378
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 134, 136
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr19:55659676-55659727

Rank: 93	Homology Score: 8	RVD Match Score: 2.962	Left Half-Site RVD Score: 4.957	Right Half-Site RVD Score: 6.244
	Genomic Region: Intron	Closest Gene: CAMK2G
Match Type: R11R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CCCAG CCCACCTGTCTCCTGC                TGACCTGGAAA                 GCAGGAGGCAGGCTCA GGAGC-3'
	3'-GGGTC GGGTGGACAGAGGACG                ACTGGACCTTT                 CGTCCTCCGTCCGAGT CCTCG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 010-075583632
Forward Primer: 5'-GGTATAATGCCCTTCCTCAGCATG-3' Tm: 59.038
Reverse Primer: 5'-CAGGGAAGAGCCATAGTCTGTC-3' Tm: 59.258
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 134, 136
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr10:75583743-75583785

Rank: 94	Homology Score: 2	RVD Match Score: 2.962	Left Half-Site RVD Score: 3.902	Right Half-Site RVD Score: 7.564
	Genomic Region: Intron	Closest Gene: SGIP1
Match Type: R16R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-ACACT CACACCACACTCCTAT              TTCTATTCTCTGCTTG              AAAAGAATAAGGAGCA GCACA-3'
	3'-TGTGA GTGTGGTGTGAGGATA              AAGATAAGAGACGAAC              TTTTCTTATTCCTCGT CGTGT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 001-067142359
Forward Primer: 5'-GGTACCTGAAACAGAGCCAGTACA-3' Tm: 59.038
Reverse Primer: 5'-GAACCCCATATCTCAGGCTGATC-3' Tm: 59.148
Expected PCR Product Length: 359
Expected CelI Cleavage Product Sizes: 165, 194
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr1:67142498-67142545

Rank: 95	Homology Score: 10	RVD Match Score: 2.962	Left Half-Site RVD Score: 5.124	Right Half-Site RVD Score: 6.063
	Genomic Region: Intron	Closest Gene: USP43
Match Type: R28R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TCATG TGAAACTGACTCCTGA        GAAAATGTGGGCAGGAATGATTTAGCAG        GCAGGTGGAAGGTGGA GAGAT-3'
	3'-AGTAC ACTTTGACTGAGGACT        CTTTTACACCCGTCCTTACTAAATCGTC        CGTCCACCTTCCACCT CTCTA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 017-009569404
Forward Primer: 5'-GGGAATGAAAAGGACAGCAGGAC-3' Tm: 59.148
Reverse Primer: 5'-CGGCCCTGCGCGTTATTTCT-3' Tm: 59.508
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 135, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr17:9569507-9569566

Rank: 96	Homology Score: 10	RVD Match Score: 2.963	Left Half-Site RVD Score: 5.768	Right Half-Site RVD Score: 5.408
	Genomic Region: Exon	Closest Gene: C10orf99
Match Type: R17R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-AGGTA CCCAGCAGCCTCCTGT             CTCCCCTTTCAGCCTTC              ACAGCAGTGAGCTGCA ATGTT-3'
	3'-TCCAT GGGTCGTCGGAGGACA             GAGGGGAAAGTCGGAAG              TGTCGTCACTCGACGT TACAA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 010-085944463
Forward Primer: 5'-TGCAAGCTTGAGCCAGAGCC-3' Tm: 59.508
Reverse Primer: 5'-GAGGTTGGCTCATGATAGCTCTG-3' Tm: 59.148
Expected PCR Product Length: 274
Expected CelI Cleavage Product Sizes: 142, 132
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr10:85944579-85944627

Rank: 97	Homology Score: 2	RVD Match Score: 2.965	Left Half-Site RVD Score: 5.67	Right Half-Site RVD Score: 5.516
	Genomic Region: Intergenic	Closest Gene: C6orf118
Match Type: R11R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TGAAC CTCCCCTGCCCCCTGT                TTAGAGAGACA                 AGAGGAGTGATGTATA TTCCC-3'
	3'-ACTTG GAGGGGACGGGGGACA                AATCTCTCTGT                 TCTCCTCACTACATAT AAGGG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 006-165472416
Forward Primer: 5'-CCTCTGCCTCGGGTTATATGAAG-3' Tm: 59.148
Reverse Primer: 5'-CGAAGGAATGCATTGCATGTTGGC-3' Tm: 59.038
Expected PCR Product Length: 289
Expected CelI Cleavage Product Sizes: 131, 158
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr6:165472524-165472566

Rank: 98	Homology Score: 2	RVD Match Score: 2.967	Left Half-Site RVD Score: 7.608	Right Half-Site RVD Score: 3.902
	Genomic Region: Intergenic	Closest Gene: KLHL25
Match Type: R28R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GCCAC TGCACCTGGCCTTTTT        TTTTGTTTTTTGGATAGGAGACATCCTA        ATGGGAGTGAGGTGTG GGCTT-3'
	3'-CGGTG ACGTGGACCGGAAAAA        AAAACAAAAAACCTATCCTCTGTAGGAT        TACCCTCACTCCACAC CCGAA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 015-086366341
Forward Primer: 5'-AGATATGGGGGTCTTGCCATGTTG-3' Tm: 59.038
Reverse Primer: 5'-TATGGGCGTTTCTCTAGGGAGAAG-3' Tm: 59.038
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 139, 131
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr15:86366448-86366507

Rank: 99	Homology Score: 5	RVD Match Score: 2.967	Left Half-Site RVD Score: 5.765	Right Half-Site RVD Score: 5.442
	Genomic Region: Intergenic	Closest Gene: RPS24
Match Type: R13R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TCCAT CACACCTGACTTATGG               CTGAACTACCTGA                GCAGGAGGCAGGTTGA GTTCG-3'
	3'-AGGTA GTGTGGACTGAATACC               GACTTGATGGACT                CGTCCTCCGTCCAACT CAAGC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 010-079846352
Forward Primer: 5'-GCTGCTCGAACCACACAACTAG-3' Tm: 59.258
Reverse Primer: 5'-CTTAAGAACTGTCACCCTGGAGAG-3' Tm: 59.038
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 137, 133
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr10:79846465-79846509

Rank: 100	Homology Score: 5	RVD Match Score: 2.967	Left Half-Site RVD Score: 5.044	Right Half-Site RVD Score: 6.19
	Genomic Region: Intergenic	Closest Gene: LPGAT1
Match Type: R21R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GTTGC CACACCTTAATCCAGT           CAGCAGCCATCTTTCATTTGT            GCAGGAGTGACCTGCA GCTTG-3'
	3'-CAACG GTGTGGAATTAGGTCA           GTCGTCGGTAGAAAGTAAACA            CGTCCTCACTGGACGT CGAAC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 001-211904919
Forward Primer: 5'-GCAGTGTCATGGGATCAAGGATGT-3' Tm: 59.038
Reverse Primer: 5'-TGCCTAGAACAATGGCTGGCATG-3' Tm: 59.148
Expected PCR Product Length: 273
Expected CelI Cleavage Product Sizes: 142, 131
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr1:211905033-211905085

Rank: 101	Homology Score: 5	RVD Match Score: 2.968	Left Half-Site RVD Score: 5.161	Right Half-Site RVD Score: 6.067
	Genomic Region: Intergenic	Closest Gene: KLHL32
Match Type: R14R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CAATT CACACATTACTACTGT               GGGGTTAGAATTTT               AGAAGTGTCAGGTACA TTAAT-3'
	3'-GTTAA GTGTGTAATGATGACA               CCCCAATCTTAAAA               TCTTCACAGTCCATGT AATTA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 006-097366253
Forward Primer: 5'-TACCTTCAGGCTGATATGGCTTGG-3' Tm: 59.038
Reverse Primer: 5'-CTGAATGTGAAGATGTCTCACAGTAGCC-3' Tm: 60.298
Expected PCR Product Length: 341
Expected CelI Cleavage Product Sizes: 131, 210
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr6:97366359-97366404

Rank: 102	Homology Score: 2	RVD Match Score: 2.968	Left Half-Site RVD Score: 5.385	Right Half-Site RVD Score: 5.831
	Genomic Region: Intergenic	Closest Gene: JAG2
Match Type: R10R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GGGTC TGCACCCCACCCCCAT                 CCCACCATCC                 ACAGTGGGCAGGGGCG CTCGT-3'
	3'-CCCAG ACGTGGGGTGGGGGTA                 GGGTGGTAGG                 TGTCACCCGTCCCCGC GAGCA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 014-105602839
Forward Primer: 5'-TCCATGCCATGCGTGGCTGT-3' Tm: 59.508
Reverse Primer: 5'-ACGGTAAGGCCTGGGGAAAC-3' Tm: 59.508
Expected PCR Product Length: 317
Expected CelI Cleavage Product Sizes: 185, 132
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr14:105603001-105603042

Rank: 103	Homology Score: 10	RVD Match Score: 2.97	Left Half-Site RVD Score: 5.441	Right Half-Site RVD Score: 5.785
	Genomic Region: Intron	Closest Gene: ANK1
Match Type: R15R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TATAA TGAACCTGGCTCCCGT              TCCTTTTATATAAGT               AGGGGAGTGAGGAGGA GGGGA-3'
	3'-ATATT ACTTGGACCGAGGGCA              AGGAAAATATATTCA               TCCCCTCACTCCTCCT CCCCT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 008-041570215
Forward Primer: 5'-GGGTGCAGAGAGATTTGCATGTG-3' Tm: 59.148
Reverse Primer: 5'-TGTCTCCTCCTGTCCCTGCT-3' Tm: 59.508
Expected PCR Product Length: 289
Expected CelI Cleavage Product Sizes: 153, 136
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr8:41570343-41570389

Rank: 104	Homology Score: 5	RVD Match Score: 2.97	Left Half-Site RVD Score: 3.923	Right Half-Site RVD Score: 7.608
	Genomic Region: Intergenic	Closest Gene: PTCHD4
Match Type: R11R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TCAAA TACCCCTCACTCCTAT                TATTAACATTG                 ACAAGAGCATGGTACA TGTAT-3'
	3'-AGTTT ATGGGGAGTGAGGATA                ATAATTGTAAC                 TGTTCTCGTACCATGT ACATA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Warning! Primer pair was generated using suboptimal parameters. 
Primer Index: 006-048618042
Forward Primer: 5'-CCTCCTGTCTTTATTAGTCTAGCAAGAAGTTTGT-3' Tm: 59.668
Reverse Primer: 5'-GTGGTGTCCTGGATAAAATCCTGAAACAGA-3' Tm: 60.058
Expected PCR Product Length: 430
Expected CelI Cleavage Product Sizes: 280, 150
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr6:48618299-48618341

Rank: 105	Homology Score: 13	RVD Match Score: 2.97	Left Half-Site RVD Score: 4.86	Right Half-Site RVD Score: 6.424
	Genomic Region: Intergenic	Closest Gene: FAM69C
Match Type: R26R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CCTAG TTCACTTCACTCCTGT         TATCCACTAAGCTCATTATCACATCG         CCAGAAGTGAGGTCCA TTTGT-3'
	3'-GGATC AAGTGAAGTGAGGACA         ATAGGTGATTCGAGTAATAGTGTAGC         GGTCTTCACTCCAGGT AAACA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 018-072086920
Forward Primer: 5'-GCTGTCACCAGGAAAAACATAAGAGCC-3' Tm: 60.438
Reverse Primer: 5'-AAGCTGCTGAAATGGGCAGAGG-3' Tm: 59.258
Expected PCR Product Length: 289
Expected CelI Cleavage Product Sizes: 159, 130
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr18:72087048-72087105

Rank: 106	Homology Score: 5	RVD Match Score: 2.971	Left Half-Site RVD Score: 5.479	Right Half-Site RVD Score: 5.759
	Genomic Region: Intergenic	Closest Gene: CYP4F35P
Match Type: R20R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GGTGC CTCTACTGACTCCTAT            CCCTGTTGCTAATCTTCCTC            AGAGGAGTAAAGTTCA TTGAG-3'
	3'-CCACG GAGATGACTGAGGATA            GGGACAACGATTAGAAGGAG            TCTCCTCATTTCAAGT AACTC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 018-014372203
Forward Primer: 5'-CCTTGGGAACCTTGGACAATAAGC-3' Tm: 59.038
Reverse Primer: 5'-GTCCCCTCTCCTTGCACTTTTC-3' Tm: 59.258
Expected PCR Product Length: 337
Expected CelI Cleavage Product Sizes: 176, 161
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr18:14372351-14372402

Rank: 107	Homology Score: 5	RVD Match Score: 2.971	Left Half-Site RVD Score: 5.759	Right Half-Site RVD Score: 5.479
	Genomic Region: Intergenic	Closest Gene: ANKRD20A9P
Match Type: R20R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CTCAA TGAACTTTACTCCTCT            GAGGAAGGTTAGCAATGGGG            ATAGGAGTCAGTAGAG GCACC-3'
	3'-GAGTT ACTTGAAATGAGGAGA            CTCCTTCCAATCGTTACCCC            TATCCTCAGTCATCTC CGTGG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 013-019260513
Forward Primer: 5'-GTCCCCTCTCCTTGCACTTTTC-3' Tm: 59.258
Reverse Primer: 5'-CTTGGGAGCCTTGGACAATAAGC-3' Tm: 59.148
Expected PCR Product Length: 335
Expected CelI Cleavage Product Sizes: 165, 170
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr13:19260650-19260701

Rank: 108	Homology Score: 2	RVD Match Score: 2.972	Left Half-Site RVD Score: 4.741	Right Half-Site RVD Score: 6.571
	Genomic Region: Intergenic	Closest Gene: ACSF3
Match Type: R27R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GGCAA CACACATCCCTCCTGT        CAGGAGCAGACATAGCCAAGAAAGCCC         AGAGCAGGCGGGGGCA GAGAG-3'
	3'-CCGTT GTGTGTAGGGAGGACA        GTCCTCGTCTGTATCGGTTCTTTCGGG         TCTCGTCCGCCCCCGT CTCTC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 016-089119131
Forward Primer: 5'-CTCGCGGCTGAGTATAGGCA-3' Tm: 59.508
Reverse Primer: 5'-GTTTCCTCTTCTTCTTCCTGGTGG-3' Tm: 59.038
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 134, 136
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr16:89119234-89119292

Rank: 109	Homology Score: 8	RVD Match Score: 2.972	Left Half-Site RVD Score: 6.792	Right Half-Site RVD Score: 4.559
	Genomic Region: Intron	Closest Gene: ATP2B2
Match Type: R19R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GGACA TGCAGCTGACACTGGT            ACTTTCTCCTCTTCTGGAC             AAGGGAGTGAGGTGTA GCCAG-3'
	3'-CCTGT ACGTCGACTGTGACCA            TGAAAGAGGAGAAGACCTG             TTCCCTCACTCCACAT CGGTC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 003-010453381
Forward Primer: 5'-TGTTGCAGACACTGGAACAGAGC-3' Tm: 59.148
Reverse Primer: 5'-CGTGAGAGTGGACTTGCAAGTCTG-3' Tm: 60.908
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 135, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr3:10453489-10453539

Rank: 110	Homology Score: 10	RVD Match Score: 2.972	Left Half-Site RVD Score: 5.472	Right Half-Site RVD Score: 5.77
	Genomic Region: Intron	Closest Gene: PIBF1
Match Type: R23R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-AAATT TACCCCTCTCTCCTCT          GAATTTAGAACTATTAAGGAGAA           ACAAGAGTCAGTTACA TATCT-3'
	3'-TTTAA ATGGGGAGAGAGGAGA          CTTAAATCTTGATAATTCCTCTT           TGTTCTCAGTCAATGT ATAGA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 013-073489472
Forward Primer: 5'-GCGCTCTGCTTCTAAGACACATTG-3' Tm: 59.038
Reverse Primer: 5'-CACATGGAAGAGATGATACATGCCATTACC-3' Tm: 60.058
Expected PCR Product Length: 386
Expected CelI Cleavage Product Sizes: 243, 143
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr13:73489686-73489740

Rank: 111	Homology Score: 10	RVD Match Score: 2.973	Left Half-Site RVD Score: 6.646	Right Half-Site RVD Score: 4.688
	Genomic Region: Intron	Closest Gene: CDC42BPA
Match Type: R26R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-ACTTC TGCAGCTGACTCAACA         ATCTGGGTTTCAACCACCCTTCCTCC         ATAGGATTCAGATGCA TGCCA-3'
	3'-TGAAG ACGTCGACTGAGTTGT         TAGACCCAAAGTTGGTGGGAAGGAGG         TATCCTAAGTCTACGT ACGGT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 001-227379954
Forward Primer: 5'-GAGATCACTCTCACCACCCAAAAC-3' Tm: 59.038
Reverse Primer: 5'-GGAAACAGCAGAACGTTGTTGGTG-3' Tm: 59.038
Expected PCR Product Length: 383
Expected CelI Cleavage Product Sizes: 190, 193
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr1:227380113-227380170

Rank: 112	Homology Score: 5	RVD Match Score: 2.973	Left Half-Site RVD Score: 5.701	Right Half-Site RVD Score: 5.551
	Genomic Region: Intergenic	Closest Gene: NKX6-1
Match Type: R29R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CAATT TTAACCTGACTCCATT       TTTCACTTAGCATTGTTCTAAACAATAAA        ACATGAGTTTAGTGTA TTTAT-3'
	3'-GTTAA AATTGGACTGAGGTAA       AAAGTGAATCGTAACAAGATTTGTTATTT        TGTACTCAAATCACAT AAATA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 004-085199195
Forward Primer: 5'-CAGTTCTACTTCAGGGAAGGGG-3' Tm: 59.258
Reverse Primer: 5'-GGATACAAGTGGAAAAGCACAGCC-3' Tm: 59.038
Expected PCR Product Length: 364
Expected CelI Cleavage Product Sizes: 196, 168
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr4:85199359-85199419

Rank: 113	Homology Score: 8	RVD Match Score: 2.974	Left Half-Site RVD Score: 6.288	Right Half-Site RVD Score: 5.006
	Genomic Region: Intergenic	Closest Gene: USP22
Match Type: R10R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TACAA TGCATATGACAACTGT                 TACACAAGGG                 ACAGGAAGGAGGTGTA AATGG-3'
	3'-ATGTT ACGTATACTGTTGACA                 ATGTGTTCCC                 TGTCCTTCCTCCACAT TTACC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 017-020954067
Forward Primer: 5'-CGGGAAAAAGTACCAGATGGAGAC-3' Tm: 59.038
Reverse Primer: 5'-GACAGAGTGAGACCCTGTCTCAA-3' Tm: 59.148
Expected PCR Product Length: 346
Expected CelI Cleavage Product Sizes: 153, 193
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr17:20954197-20954238

Rank: 114	Homology Score: 2	RVD Match Score: 2.975	Left Half-Site RVD Score: 5.516	Right Half-Site RVD Score: 5.751
	Genomic Region: Intergenic	Closest Gene: GBE1
Match Type: R18R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-AAACC TAGACCTACCACCTGT             GTTCCACCAAATTTATTG             GTAGGAGGAAGGGGCA AAGGG-3'
	3'-TTTGG ATCTGGATGGTGGACA             CAAGGTGGTTTAAATAAC             CATCCTCCTTCCCCGT TTCCC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 003-082432463
Forward Primer: 5'-GACAGGGAAGAAACTCTGCAAACC-3' Tm: 59.038
Reverse Primer: 5'-GCACGGATATGCAATAAACTTCTGTTTCCC-3' Tm: 60.058
Expected PCR Product Length: 395
Expected CelI Cleavage Product Sizes: 134, 261
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr3:82432570-82432619

Rank: 115	Homology Score: 2	RVD Match Score: 2.977	Left Half-Site RVD Score: 5.502	Right Half-Site RVD Score: 5.78
	Genomic Region: Intergenic	Closest Gene: KLF8
Match Type: R24R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TTTAT TCAACCAAACACCTGT          ATTTTTCCCCAGTATTTCACTAAT          ATAAAAGTGAGGTGGA TGACT-3'
	3'-AAATA AGTTGGTTTGTGGACA          TAAAAAGGGGTCATAAAGTGATTA          TATTTTCACTCCACCT ACTGA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 00X-056339296
Forward Primer: 5'-GCAAGAATATTGGCAATACTGTCCCATGTC-3' Tm: 60.058
Reverse Primer: 5'-GCAGAGGCCTAGTCTGAAGC-3' Tm: 59.508
Expected PCR Product Length: 396
Expected CelI Cleavage Product Sizes: 132, 264
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chrX:56339398-56339453

Rank: 116	Homology Score: 2	RVD Match Score: 2.977	Left Half-Site RVD Score: 5.78	Right Half-Site RVD Score: 5.502
	Genomic Region: Intergenic	Closest Gene: ZC3H12C
Match Type: R24R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-AGTCA TCCACCTCACTTTTAT          ATTAGTGAAATACTGGGGAAAATT          ACAGGTGTTTGGTTGA ATAAA-3'
	3'-TCAGT AGGTGGAGTGAAAATA          TAATCACTTTATGACCCCTTTTAA          TGTCCACAAACCAACT TATTT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Warning! Primer pair was generated using suboptimal parameters. 
Primer Index: 011-109690484
Forward Primer: 5'-GCAGAGGCCTAGTCTGAAGC-3' Tm: 59.508
Reverse Primer: 5'-AAGCAAGAATATTGGCAATACTGTCCCATGTC-3' Tm: 59.848
Expected PCR Product Length: 398
Expected CelI Cleavage Product Sizes: 268, 130
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr11:109690722-109690777

Rank: 117	Homology Score: 2	RVD Match Score: 2.978	Left Half-Site RVD Score: 5.154	Right Half-Site RVD Score: 6.153
	Genomic Region: Intergenic	Closest Gene: CDON
Match Type: R27R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TTTCC CCCGCATGACTCCTAT        CAGAGGCCTAGGGTGCTGGGCAGAGGC         TCTGGAGTGAGGAGGA GTTAG-3'
	3'-AAAGG GGGCGTACTGAGGATA        GTCTCCGGATCCCACGACCCGTCTCCG         AGACCTCACTCCTCCT CAATC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 011-125968061
Forward Primer: 5'-GACACTAGAAATCTGGGTACCCTG-3' Tm: 59.038
Reverse Primer: 5'-ACAGCTGTTTGCTCCGTGGG-3' Tm: 59.508
Expected PCR Product Length: 283
Expected CelI Cleavage Product Sizes: 134, 149
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr11:125968164-125968222

Rank: 118	Homology Score: 5	RVD Match Score: 2.979	Left Half-Site RVD Score: 5.13	Right Half-Site RVD Score: 6.188
	Genomic Region: Intron	Closest Gene: KLHL14
Match Type: R11R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CCTAG CACCCATGCCTCCTGT                AAAGCCGTCAC                 AAACGTTTCAGGTGCA AGAAA-3'
	3'-GGATC GTGGGTACGGAGGACA                TTTCGGCAGTG                 TTTGCAAAGTCCACGT TCTTT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 018-030266951
Forward Primer: 5'-GAATTAACAAGGGAGGTCATGTAAACTGGC-3' Tm: 60.058
Reverse Primer: 5'-CGTGCTGTGTTAGGTATTCCCTC-3' Tm: 59.148
Expected PCR Product Length: 384
Expected CelI Cleavage Product Sizes: 131, 253
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr18:30267059-30267101

Rank: 119	Homology Score: 8	RVD Match Score: 2.979	Left Half-Site RVD Score: 6.098	Right Half-Site RVD Score: 5.216
	Genomic Region: Intergenic	Closest Gene: KHDRBS2
Match Type: R18R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-AGCAC TGCAACTCATTCCTGG             TGCCCAGTGGCTCCTAGG             AAAGGGGTGAGGTGAA CAGGT-3'
	3'-TCGTG ACGTTGAGTAAGGACC             ACGGGTCACCGAGGATCC             TTTCCCCACTCCACTT GTCCA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 006-063449762
Forward Primer: 5'-TCTGAGGAGACCTTCAGAGGG-3' Tm: 59.378
Reverse Primer: 5'-ACCACCTCTTGAAGCAGCTCTC-3' Tm: 59.258
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 135, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr6:63449870-63449919

Rank: 120	Homology Score: 17	RVD Match Score: 2.98	Left Half-Site RVD Score: 4.129	Right Half-Site RVD Score: 7.408
	Genomic Region: Intron	Closest Gene: CARD11
Match Type: R17R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GGGGG TGCACCTGCCTCCTGC             CTGCACCCTGGGAGCTT              GCAGGATGCAGAAGCA TGGGC-3'
	3'-CCCCC ACGTGGACGGAGGACG             GACGTGGGACCCTCGAA              CGTCCTACGTCTTCGT ACCCG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 007-002962408
Forward Primer: 5'-ACAGCGTTTGTCTCCTGGCC-3' Tm: 59.508
Reverse Primer: 5'-GCCCCTAAGCCTTGCATTCAG-3' Tm: 59.378
Expected PCR Product Length: 298
Expected CelI Cleavage Product Sizes: 167, 131
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr7:2962549-2962597

Rank: 121	Homology Score: 2	RVD Match Score: 2.98	Left Half-Site RVD Score: 5.796	Right Half-Site RVD Score: 5.507
	Genomic Region: Intron	Closest Gene: CNTN5
Match Type: R30R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-ATACC CTCTCCTGTCTCCTAT       TTAGGTTGATAAAAGTGCCACCCCTCCTTC       ATAGGATTTAGATGAA ATTGA-3'
	3'-TATGG GAGAGGACAGAGGATA       AATCCAACTATTTTCACGGTGGGGAGGAAG       TATCCTAAATCTACTT TAACT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 011-099262817
Forward Primer: 5'-CTTGGACTACCCTAAGGCTCAG-3' Tm: 59.258
Reverse Primer: 5'-TCAGATGGGCTGGATGAAAAGTTAGCCT-3' Tm: 60.298
Expected PCR Product Length: 427
Expected CelI Cleavage Product Sizes: 297, 130
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr11:99263081-99263142

Rank: 122	Homology Score: 8	RVD Match Score: 2.981	Left Half-Site RVD Score: 5.281	Right Half-Site RVD Score: 6.042
	Genomic Region: Intron	Closest Gene: ZFPM1
Match Type: R29R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-ACGCC TTCACCTGCCTCCTCA       AGTCAGAGCTGTGAGCCAGCCTGCCAGGG        CCAGGGCTCAGGTGGA GAAAC-3'
	3'-TGCGG AAGTGGACGGAGGAGT       TCAGTCTCGACACTCGGTCGGACGGTCCC        GGTCCCGAGTCCACCT CTTTG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 016-088575852
Forward Primer: 5'-CCATTAGGCACACAGGGAGG-3' Tm: 59.508
Reverse Primer: 5'-CTGCCTCCTGAACCCACTCT-3' Tm: 59.508
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 135, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr16:88575955-88576015

Rank: 123	Homology Score: 2	RVD Match Score: 2.982	Left Half-Site RVD Score: 5.87	Right Half-Site RVD Score: 5.447
	Genomic Region: Intergenic	Closest Gene: VIPR2
Match Type: R27R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GTTTA TGCACCTAACAACAGA        GCTTGAAAATACATGAAGCAAATACTG         ATAGAAGTGAGATGTA ATCTA-3'
	3'-CAAAT ACGTGGATTGTTGTCT        CGAACTTTTATGTACTTCGTTTATGAC         TATCTTCACTCTACAT TAGAT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 007-158980941
Forward Primer: 5'-GCCCAACTACATGCTGGGTC-3' Tm: 59.508
Reverse Primer: 5'-GTGGCCTATCTTGATGAATGTCCC-3' Tm: 59.038
Expected PCR Product Length: 422
Expected CelI Cleavage Product Sizes: 200, 222
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr7:158981110-158981168

Rank: 124	Homology Score: 5	RVD Match Score: 2.982	Left Half-Site RVD Score: 6.129	Right Half-Site RVD Score: 5.205
	Genomic Region: Intergenic	Closest Gene: ZC3H4
Match Type: R14R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CTGCC CCCATCTGACTGCTGC               TTTGACTAGAGCAA               ACTTGAGTCAGGTTTA TCTCC-3'
	3'-GACGG GGGTAGACTGACGACG               AAACTGATCTCGTT               TGAACTCAGTCCAAAT AGAGG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 019-047560548
Forward Primer: 5'-CTGCGTTGCCTGGGATAGTC-3' Tm: 59.508
Reverse Primer: 5'-GCTATGTGGGGTCAGGCAGT-3' Tm: 59.508
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 135, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr19:47560658-47560703

Rank: 125	Homology Score: 5	RVD Match Score: 2.982	Left Half-Site RVD Score: 4.904	Right Half-Site RVD Score: 6.468
	Genomic Region: Intergenic	Closest Gene: KCNF1
Match Type: R12R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CCCAA CCCACCTGACTTCTGA                GTACTTCCTTTC                AGTGGGATCTGGTGCA GCAGT-3'
	3'-GGGTT GGGTGGACTGAAGACT                CATGAAGGAAAG                TCACCCTAGACCACGT CGTCA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 002-011017617
Forward Primer: 5'-GAGCTTGGTGAATAGAAGGTACCC-3' Tm: 59.038
Reverse Primer: 5'-CTCCCCCAAGCACTCCATTTAAC-3' Tm: 59.148
Expected PCR Product Length: 316
Expected CelI Cleavage Product Sizes: 182, 134
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr2:11017775-11017818

Rank: 126	Homology Score: 10	RVD Match Score: 2.983	Left Half-Site RVD Score: 7.259	Right Half-Site RVD Score: 4.262
	Genomic Region: Intron	Closest Gene: COL25A1
Match Type: R11R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TGCAA TGCAGCGGGCTCCCCT                TACACACCTTT                 ACAGGAGTTAGTTGAA TATCA-3'
	3'-ACGTT ACGTCGCCCGAGGGGA                ATGTGTGGAAA                 TGTCCTCAATCAACTT ATAGT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 004-110007835
Forward Primer: 5'-GACTGCTGGGAATTGCTCTTTGAC-3' Tm: 59.038
Reverse Primer: 5'-TCCCTCAACTTCCACTGGGC-3' Tm: 59.508
Expected PCR Product Length: 290
Expected CelI Cleavage Product Sizes: 157, 133
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr4:110007969-110008011

Rank: 127	Homology Score: 10	RVD Match Score: 2.983	Left Half-Site RVD Score: 6.477	Right Half-Site RVD Score: 4.904
	Genomic Region: Intergenic	Closest Gene: PTGER4P2
Match Type: R18R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GGTAA TCCACCTGCCTCGGCT             TCCCAAAGTGTTGGGGTT             ACAGGTGTGAGGTACA ACGCC-3'
	3'-CCATT AGGTGGACGGAGCCGA             AGGGTTTCACAACCCCAA             TGTCCACACTCCATGT TGCGG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 009-066282880
Forward Primer: 5'-ACTACAGGTGCACACCACCATG-3' Tm: 59.258
Reverse Primer: 5'-GCCTTCCCCAAGTTTGCATTTTCG-3' Tm: 59.038
Expected PCR Product Length: 301
Expected CelI Cleavage Product Sizes: 131, 170
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr9:66282984-66283033

Rank: 128	Homology Score: 5	RVD Match Score: 2.984	Left Half-Site RVD Score: 5.628	Right Half-Site RVD Score: 5.699
	Genomic Region: Intron	Closest Gene: ZNF304
Match Type: R28R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CAGAG CTCACCTGACACCAGC        GGCCAAGGCCCTGCTGAAACTCTCTTGC        AGAGGAATCAGGTAGA GGCTT-3'
	3'-GTCTC GAGTGGACTGTGGTCG        CCGGTTCCGGGACGACTTTGAGAGAACG        TCTCCTTAGTCCATCT CCGAA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 019-057866958
Forward Primer: 5'-GCCTCCGTGTTTCAATTGCTCTC-3' Tm: 59.148
Reverse Primer: 5'-GGGCTGGTTGTCAGTATAGGG-3' Tm: 59.378
Expected PCR Product Length: 302
Expected CelI Cleavage Product Sizes: 172, 130
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr19:57867098-57867157

Rank: 129	Homology Score: 5	RVD Match Score: 2.984	Left Half-Site RVD Score: 5.266	Right Half-Site RVD Score: 6.076
	Genomic Region: Intron	Closest Gene: TTC40
Match Type: R27R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GGACA TGCACCTGACCCCCAG        GCAGCCAGCAGCACACGCCCATGAGTG         AAAGGAGTCAGGAAGG CAAAA-3'
	3'-CCTGT ACGTGGACTGGGGGTC        CGTCGGTCGTCGTGTGCGGGTACTCAC         TTTCCTCAGTCCTTCC GTTTT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 010-134734250
Forward Primer: 5'-ACTCAACGGCATCCTCCCCT-3' Tm: 59.508
Reverse Primer: 5'-ATCCACCGCCCTCAGATGCT-3' Tm: 59.508
Expected PCR Product Length: 278
Expected CelI Cleavage Product Sizes: 146, 132
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr10:134734365-134734423

Rank: 130	Homology Score: 17	RVD Match Score: 2.985	Left Half-Site RVD Score: 6.923	Right Half-Site RVD Score: 4.543
	Genomic Region: Intergenic	Closest Gene: SLC2A10
Match Type: R24R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CCCCT TCCTCCAGCTTCCTGT          AGGTTCAACTAGGGGGAAGCCCCA          GCAGGAGACAGGTGCA CAGTG-3'
	3'-GGGGA AGGAGGTCGAAGGACA          TCCAAGTTGATCCCCCTTCGGGGT          CGTCCTCTGTCCACGT GTCAC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 020-045428486
Forward Primer: 5'-GTCTCACTGAGACCTAGGCG-3' Tm: 59.508
Reverse Primer: 5'-TGGGCCTGGGGAACCAACAA-3' Tm: 59.508
Expected PCR Product Length: 268
Expected CelI Cleavage Product Sizes: 133, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr20:45428589-45428644

Rank: 131	Homology Score: 5	RVD Match Score: 2.985	Left Half-Site RVD Score: 5.538	Right Half-Site RVD Score: 5.804
	Genomic Region: Intron	Closest Gene: YES1
Match Type: R11R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-ACTAT TCCAACTGACCCATGT                GAGAAAAGGCA                 AAAGCAGCCAGGTGTG GCGGC-3'
	3'-TGATA AGGTTGACTGGGTACA                CTCTTTTCCGT                 TTTCGTCGGTCCACAC CGCCG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 018-000763240
Forward Primer: 5'-CCAGGGAGCATTAGGAATCCAG-3' Tm: 59.258
Reverse Primer: 5'-TTTGTAGAGACGGGGTTCTGCC-3' Tm: 59.258
Expected PCR Product Length: 295
Expected CelI Cleavage Product Sizes: 164, 131
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr18:763381-763423

Rank: 132	Homology Score: 2	RVD Match Score: 2.987	Left Half-Site RVD Score: 5.939	Right Half-Site RVD Score: 7.469
	Genomic Region: Intergenic	Closest Gene: TICRR
Match Type: L30R
	           Left Nuclease
	         TCACCTTGCCCCACAGGGCAGT
	5'-GCCCG TCACCTTGCCCAACCCAACAGT CCATTCTCAGCCTTCATTTTATCCCACCTC       ACAGCAGCCTGTGGCA GAGGG-3'
	3'-CGGGC AGTGGAACGGGTTGGGTTGTCA GGTAAGAGTCGGAAGTAAAATAGGGTGGAG       TGTCGTCGGACACCGT CTCCC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 015-090111045
Forward Primer: 5'-GCCTTCCTCTCCTCATACCC-3' Tm: 59.508
Reverse Primer: 5'-TACAGGTGTGAGCCACCACG-3' Tm: 59.508
Expected PCR Product Length: 273
Expected CelI Cleavage Product Sizes: 131, 142
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr15:90111140-90111207

Rank: 133	Homology Score: 10	RVD Match Score: 2.987	Left Half-Site RVD Score: 5.715	Right Half-Site RVD Score: 5.64
	Genomic Region: Exon	Closest Gene: KIAA1244
Match Type: R19R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CATTG TGTAGCCGACTCCTGT            TCTACTCTCCCACCAAATA             ACAGTAGTGAGGGTTA GAGTC-3'
	3'-GTAAC ACATCGGCTGAGGACA            AGATGAGAGGGTGGTTTAT             TGTCATCACTCCCAAT CTCAG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 006-138657515
Forward Primer: 5'-GTTCCCGTGCATCAGTCAGC-3' Tm: 59.508
Reverse Primer: 5'-GCTGATCAGTGGTTGGAAAGGC-3' Tm: 59.258
Expected PCR Product Length: 272
Expected CelI Cleavage Product Sizes: 131, 141
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr6:138657619-138657669

Rank: 134	Homology Score: 10	RVD Match Score: 2.987	Left Half-Site RVD Score: 6.983	Right Half-Site RVD Score: 4.507
	Genomic Region: Promoter	Closest Gene: C1orf198
Match Type: R11R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CTCTC TCCATCTGACTGATTT                TAACAAGACTA                 ACAGGACTCAGGTGAG AATTG-3'
	3'-GAGAG AGGTAGACTGACTAAA                ATTGTTCTGAT                 TGTCCTGAGTCCACTC TTAAC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 001-231005091
Forward Primer: 5'-GCGACATCCCAACAGGTAGG-3' Tm: 59.508
Reverse Primer: 5'-CGGAAGATGAGGCCATCTCAC-3' Tm: 59.378
Expected PCR Product Length: 298
Expected CelI Cleavage Product Sizes: 131, 167
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr1:231005199-231005241

Rank: 135	Homology Score: 13	RVD Match Score: 2.988	Left Half-Site RVD Score: 6.01	Right Half-Site RVD Score: 5.362
	Genomic Region: Intergenic	Closest Gene: ATP8B1
Match Type: R11R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-AGGGC TGCACGTGACTTCTGG                GGCAAAGGTAA                 GTAGGAGAAAGGTGCA CCTTC-3'
	3'-TCCCG ACGTGCACTGAAGACC                CCGTTTCCATT                 CATCCTCTTTCCACGT GGAAG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 018-055555293
Forward Primer: 5'-TCTGGGAAGAAGCTGCCAGG-3' Tm: 59.508
Reverse Primer: 5'-GGCTATGCTACCTGCTGTAAAACC-3' Tm: 59.038
Expected PCR Product Length: 342
Expected CelI Cleavage Product Sizes: 134, 208
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr18:55555404-55555446

Rank: 136	Homology Score: 5	RVD Match Score: 2.99	Left Half-Site RVD Score: 5.325	Right Half-Site RVD Score: 6.063
	Genomic Region: Intergenic	Closest Gene: DOT1L
Match Type: R19R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GCCAC CGCACCCGGCCCCTGT            TGAGATCTTGATCAACACT             AAAGGGGCCGGGTGCG GTGGC-3'
	3'-CGGTG GCGTGGGCCGGGGACA            ACTCTAGAACTAGTTGTGA             TTTCCCCGGCCCACGC CACCG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 019-002162216
Forward Primer: 5'-TAGTAGAGACGGGGTTTCACCATG-3' Tm: 59.038
Reverse Primer: 5'-TTTCCGCCATGTTGGCCAGG-3' Tm: 59.508
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 135, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr19:2162324-2162374

Rank: 137	Homology Score: 5	RVD Match Score: 2.99	Left Half-Site RVD Score: 5.538	Right Half-Site RVD Score: 5.84
	Genomic Region: Intron	Closest Gene: RNF128
Match Type: R13R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CGATG TCCAACTGACTCATGA               TCTATGTGGAAAG                AAAGTAGTGAAGTGTA GGTGT-3'
	3'-GCTAC AGGTTGACTGAGTACT               AGATACACCTTTC                TTTCATCACTTCACAT CCACA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 00X-106025404
Forward Primer: 5'-CTTTCCCCAACTGTGTGATACAGC-3' Tm: 59.038
Reverse Primer: 5'-CTGGTACTGGAACTCTGCTCTTCA-3' Tm: 59.038
Expected PCR Product Length: 380
Expected CelI Cleavage Product Sizes: 191, 189
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chrX:106025571-106025615

Rank: 138	Homology Score: 2	RVD Match Score: 2.991	Left Half-Site RVD Score: 5.362	Right Half-Site RVD Score: 6.031
	Genomic Region: Intergenic	Closest Gene: EGR1
Match Type: R30R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TTCCT TCCACCTTCCCCCTAT       TCAGAACCGCCCCTATCCCCCAGGTATGGA       ACTGGAGGCAGGTAAG AGTAC-3'
	3'-AAGGA AGGTGGAAGGGGGATA       AGTCTTGGCGGGGATAGGGGGTCCATACCT       TGACCTCCGTCCATTC TCATG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 005-137808181
Forward Primer: 5'-CGTTCAGCCTCGTTCTCACC-3' Tm: 59.508
Reverse Primer: 5'-CAATGGCTTCCCCTCTCCAAC-3' Tm: 59.378
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 135, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr5:137808283-137808344

Rank: 139	Homology Score: 2	RVD Match Score: 2.992	Left Half-Site RVD Score: 5.399	Right Half-Site RVD Score: 6.001
	Genomic Region: Intergenic	Closest Gene: HSP90AA4P
Match Type: R10R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CCCCG TCCACCCCATTCCTAT                 CTGTGTTATT                 ACAGGATTGAATTGTA ATTTG-3'
	3'-GGGGC AGGTGGGGTAAGGATA                 GACACAATAA                 TGTCCTAACTTAACAT TAAAC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 004-190407488
Forward Primer: 5'-CCTGGGGGAACTTTTTCAGTCCAT-3' Tm: 59.038
Reverse Primer: 5'-GGTTTCACATGCCCTGAATACTGC-3' Tm: 59.038
Expected PCR Product Length: 407
Expected CelI Cleavage Product Sizes: 211, 196
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr4:190407676-190407717

Rank: 140	Homology Score: 5	RVD Match Score: 2.992	Left Half-Site RVD Score: 5.044	Right Half-Site RVD Score: 6.391
	Genomic Region: Intergenic	Closest Gene: SNTB1
Match Type: R27R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GTAAC TGCACATAACTCCTTA        CCTTAGCATTCTCCAACTCCAGTGCCC         ACAAGGGTCAGGTAAG TGAGT-3'
	3'-CATTG ACGTGTATTGAGGAAT        GGAATCGTAAGAGGTTGAGGTCACGGG         TGTTCCCAGTCCATTC ACTCA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 008-121927538
Forward Primer: 5'-GCAGTGATGAAATCACACTGGCC-3' Tm: 59.148
Reverse Primer: 5'-GCCAAGAAACACTAGCCACAGTC-3' Tm: 59.148
Expected PCR Product Length: 288
Expected CelI Cleavage Product Sizes: 145, 143
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr8:121927652-121927710

Rank: 141	Homology Score: 8	RVD Match Score: 2.993	Left Half-Site RVD Score: 4.632	Right Half-Site RVD Score: 6.878
	Genomic Region: Intron	Closest Gene: C3orf55
Match Type: R29R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TTTTG CACACCAGACTGCTGT       GGAGTGTCCCAGCAGAAGGTCATCCACGT        ACAGGAGCAAGACGCA GCCTA-3'
	3'-AAAAC GTGTGGTCTGACGACA       CCTCACAGGGTCGTCTTCCAGTAGGTGCA        TGTCCTCGTTCTGCGT CGGAT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 003-157302016
Forward Primer: 5'-ATCCCAGGTAGCGTACCTGC-3' Tm: 59.508
Reverse Primer: 5'-CTTAGTACACTTGGACCTGGCTTC-3' Tm: 59.038
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 134, 136
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr3:157302118-157302178

Rank: 142	Homology Score: 13	RVD Match Score: 2.993	Left Half-Site RVD Score: 4.632	Right Half-Site RVD Score: 6.878
	Genomic Region: Intergenic	Closest Gene: ZNF812
Match Type: R29R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TTTTG TGCACCCAACTGCTGT       GGAGTGTCCCAGCAGAACGTCGTCCATGT        ACAGGAGCAAGACGCA GCCTA-3'
	3'-AAAAC ACGTGGGTTGACGACA       CCTCACAGGGTCGTCTTGCAGCAGGTACA        TGTCCTCGTTCTGCGT CGGAT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 019-009837811
Forward Primer: 5'-AATCCCAGGTAGCGTACGTGC-3' Tm: 59.378
Reverse Primer: 5'-TCAGTACACATGGACCTGGCTTC-3' Tm: 59.148
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 135, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr19:9837914-9837974

Rank: 143	Homology Score: 8	RVD Match Score: 2.994	Left Half-Site RVD Score: 5.695	Right Half-Site RVD Score: 5.716
	Genomic Region: Intergenic	Closest Gene: YIPF5
Match Type: R12R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-ACTGC TTCACCTGTCTCCTCC                CATTATAAATCT                TCAGGAGTCCTGTGGA GGTGG-3'
	3'-TGACG AAGTGGACAGAGGAGG                GTAATATTTAGA                AGTCCTCAGGACACCT CCACC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 005-143474515
Forward Primer: 5'-CCACTCTCCCTCAAATCAACTGC-3' Tm: 59.148
Reverse Primer: 5'-CTGCATTTCTGAGACAGACATGGC-3' Tm: 59.038
Expected PCR Product Length: 351
Expected CelI Cleavage Product Sizes: 202, 149
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr5:143474693-143474736

Rank: 144	Homology Score: 5	RVD Match Score: 2.995	Left Half-Site RVD Score: 5.642	Right Half-Site RVD Score: 5.776
	Genomic Region: Intergenic	Closest Gene: ZPLD1
Match Type: R17R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CCTTC TCCACCTTACTCTTGC             TGACAGGAGGAGATTAG              AGGGGAGTTAGGAGAA GGAAG-3'
	3'-GGAAG AGGTGGAATGAGAACG             ACTGTCCTCCTCTAATC              TCCCCTCAATCCTCTT CCTTC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 003-102226111
Forward Primer: 5'-CCAGCATCATTGAAAGCTGGCAGA-3' Tm: 59.038
Reverse Primer: 5'-CTGTATCCCCATCATGTGCTGAAG-3' Tm: 59.038
Expected PCR Product Length: 413
Expected CelI Cleavage Product Sizes: 268, 145
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr3:102226353-102226401

Rank: 145	Homology Score: 10	RVD Match Score: 2.995	Left Half-Site RVD Score: 6.953	Right Half-Site RVD Score: 4.588
	Genomic Region: Intergenic	Closest Gene: LRP2
Match Type: R13R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GAACG TGCACCAGAATTCAGC               CAAGCTCCCTATC                AAAGGAGTCAGGTCTA TCTTT-3'
	3'-CTTGC ACGTGGTCTTAAGTCG               GTTCGAGGGATAG                TTTCCTCAGTCCAGAT AGAAA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 002-170227380
Forward Primer: 5'-GGCCAGAAGACCAAGATGATTCC-3' Tm: 59.148
Reverse Primer: 5'-GCCAAAAGCCAAATGTAGACTTTGTGCTG-3' Tm: 60.178
Expected PCR Product Length: 403
Expected CelI Cleavage Product Sizes: 259, 144
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr2:170227615-170227659

Rank: 146	Homology Score: 5	RVD Match Score: 2.996	Left Half-Site RVD Score: 6.878	Right Half-Site RVD Score: 4.653
	Genomic Region: Intron	Closest Gene: STK24
Match Type: R30R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TGTTC TGCCCTCGCCACCTGT       CACAATTTCTGGAAGGCATTCGAAGGAGAC       ACAGGAGTCAGGGCTG GTGGG-3'
	3'-ACAAG ACGGGAGCGGTGGACA       GTGTTAAAGACCTTCCGTAAGCTTCCTCTG       TGTCCTCAGTCCCGAC CACCC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 013-099191073
Forward Primer: 5'-AGGCCCTGGTGTCCCTTCTT-3' Tm: 59.508
Reverse Primer: 5'-TGATGGAAACAGCGGGTGCTC-3' Tm: 59.378
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 132, 138
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr13:99191172-99191233

Rank: 147	Homology Score: 13	RVD Match Score: 2.999	Left Half-Site RVD Score: 6.01	Right Half-Site RVD Score: 5.441
	Genomic Region: Intergenic	Closest Gene: GPC1
Match Type: R29R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GAGCC TTCACCTGATTCCAGC       CGCAAAGCCCTTTCCAGTACGGCCCATTC        ACAGGTTCCAGGTGCA AAGCC-3'
	3'-CTCGG AAGTGGACTAAGGTCG       GCGTTTCGGGAAAGGTCATGCCGGGTAAG        TGTCCAAGGTCCACGT TTCGG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 002-241334168
Forward Primer: 5'-CATGCACCCCTGGCTTGTGT-3' Tm: 59.508
Reverse Primer: 5'-AGCTTTGCACCTGGAACCTGTGA-3' Tm: 59.148
Expected PCR Product Length: 297
Expected CelI Cleavage Product Sizes: 138, 159
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr2:241334274-241334334

Rank: 148	Homology Score: 5	RVD Match Score: 2.999	Left Half-Site RVD Score: 6.265	Right Half-Site RVD Score: 5.205
	Genomic Region: Intergenic	Closest Gene: CENPC1
Match Type: R27R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-AAGCG TGCATCTAAATCTTCT        AAACAATCAGAATTCCAGGTTTCTGGG         ATATTTGTCAGGTGCA AAATG-3'
	3'-TTCGC ACGTAGATTTAGAAGA        TTTGTTAGTCTTAAGGTCCAAAGACCC         TATAAACAGTCCACGT TTTAC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 004-068122758
Forward Primer: 5'-CTTTTGCTTGTTGGTTCGGTGGC-3' Tm: 59.148
Reverse Primer: 5'-GTCCTGATGGAGTCTAAATGGCTG-3' Tm: 59.038
Expected PCR Product Length: 281
Expected CelI Cleavage Product Sizes: 134, 147
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr4:68122861-68122919

Rank: 149	Homology Score: 13	RVD Match Score: 2.999	Left Half-Site RVD Score: 4.632	Right Half-Site RVD Score: 6.931
	Genomic Region: Intergenic	Closest Gene: CTSB
Match Type: R29R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CTTGG TGCACCTGACTGCCAT       GGGGTGTCCCAGCAAAAGGGCATCAATCT        ACTGGAGCCACATGCA GTCTA-3'
	3'-GAACC ACGTGGACTGACGGTA       CCCCACAGGGTCGTTTTCCCGTAGTTAGA        TGACCTCGGTGTACGT CAGAT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 008-011765812
Forward Primer: 5'-ATCCCCAGTAATGTACCTGCTGTC-3' Tm: 59.038
Reverse Primer: 5'-CTCAGTACACTTGCACCCGG-3' Tm: 59.508
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 134, 136
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr8:11765914-11765974

Rank: 150	Homology Score: 10	RVD Match Score: 2.999	Left Half-Site RVD Score: 5.884	Right Half-Site RVD Score: 5.567
	Genomic Region: Intergenic	Closest Gene: FLJ43860
Match Type: R24R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TTTGT TAAACCACATTCCTGT          GAGAGGCAACTTTTCAACTGGAGA          ACAGTACTCATGTGCA GTTCC-3'
	3'-AAACA ATTTGGTGTAAGGACA          CTCTCCGTTGAAAAGTTGACCTCT          TGTCATGAGTACACGT CAAGG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 008-142885698
Forward Primer: 5'-GTGAACTACCTCTCTGGCACTG-3' Tm: 59.258
Reverse Primer: 5'-CTCACCCAAAACTGGAAGCAACC-3' Tm: 59.148
Expected PCR Product Length: 336
Expected CelI Cleavage Product Sizes: 132, 204
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr8:142885800-142885855

Rank: 151	Homology Score: 17	RVD Match Score: 2.999	Left Half-Site RVD Score: 4.607	Right Half-Site RVD Score: 6.963
	Genomic Region: Intron	Closest Gene: CABIN1
Match Type: R10R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GGGGT TGCATCTGAATCCTGT                 GGGTGTGGCA                 GCAGGGGACAGCTGGA AAAGG-3'
	3'-CCCCA ACGTAGACTTAGGACA                 CCCACACCGT                 CGTCCCCTGTCGACCT TTTCC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 022-024555541
Forward Primer: 5'-CACAGCCATTCTGTCTACTCCC-3' Tm: 59.258
Reverse Primer: 5'-TGGCAGGTGGCCAGTGTCTT-3' Tm: 59.508
Expected PCR Product Length: 273
Expected CelI Cleavage Product Sizes: 140, 133
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr22:24555658-24555699

Rank: 152	Homology Score: 10	RVD Match Score: 3	Left Half-Site RVD Score: 4.963	Right Half-Site RVD Score: 6.542
	Genomic Region: Intergenic	Closest Gene: PPARGC1A
Match Type: R13R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-ACAAA TGCACCCTACTCATGT               AAGATGTTATAAC                AGGGGAGACTGGGGCA GGGCA-3'
	3'-TGTTT ACGTGGGATGAGTACA               TTCTACAATATTG                TCCCCTCTGACCCCGT CCCGT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 004-024170953
Forward Primer: 5'-CTGGATGGGATCCCAGAACAGA-3' Tm: 59.258
Reverse Primer: 5'-GAGCCAACATGCTTGGCTGAG-3' Tm: 59.378
Expected PCR Product Length: 276
Expected CelI Cleavage Product Sizes: 140, 136
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr4:24171069-24171113

Rank: 153	Homology Score: 5	RVD Match Score: 3	Left Half-Site RVD Score: 4.957	Right Half-Site RVD Score: 6.555
	Genomic Region: Intron	Closest Gene: TMPRSS4-AS1
Match Type: R11R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-AGTGG TGCCCCTCACTCCTCA                CACAGGTGCTG                 AGAGGAGGGAAGAGCA CCCAG-3'
	3'-TCACC ACGGGGAGTGAGGAGT                GTGTCCACGAC                 TCTCCTCCCTTCTCGT GGGTC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 011-117929965
Forward Primer: 5'-GCTCCGATGTGAGAAGTCTCTAG-3' Tm: 59.148
Reverse Primer: 5'-CCTTCCACACATTCTGCACTCTG-3' Tm: 59.148
Expected PCR Product Length: 274
Expected CelI Cleavage Product Sizes: 133, 141
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr11:117930075-117930117

Rank: 154	Homology Score: 2	RVD Match Score: 3.001	Left Half-Site RVD Score: 4.769	Right Half-Site RVD Score: 6.784
	Genomic Region: Intron	Closest Gene: NTRK2
Match Type: R18R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GACTC CACACCTAAATTCTGT             CCTCAAAACAGTGGGAAG             CCAGGGGTCAAGGGGA CAGTC-3'
	3'-CTGAG GTGTGGATTTAAGACA             GGAGTTTTGTCACCCTTC             GGTCCCCAGTTCCCCT GTCAG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 009-087590855
Forward Primer: 5'-CAAGGGGAAGGAGTGGTTATCTAG-3' Tm: 59.038
Reverse Primer: 5'-GTCTCAGACTTCTAGCCTCCAG-3' Tm: 59.258
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 135, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr9:87590963-87591012

Rank: 155	Homology Score: 5	RVD Match Score: 3.002	Left Half-Site RVD Score: 4.603	Right Half-Site RVD Score: 6.987
	Genomic Region: Intergenic	Closest Gene: FOXN4
Match Type: R16R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CTCTG CACACCTGACTCCCCC              TTGCCCATGGCCAGGA              ACAGAAGTCAGACTCA ACTGT-3'
	3'-GAGAC GTGTGGACTGAGGGGG              AACGGGTACCGGTCCT              TGTCTTCAGTCTGAGT TGACA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 012-109714286
Forward Primer: 5'-CCCCATGTCTCCTGTATGCTTTC-3' Tm: 59.148
Reverse Primer: 5'-ACTTTGCAGGAGCTAGGGGAC-3' Tm: 59.378
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 138, 132
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr12:109714398-109714445

Rank: 156	Homology Score: 8	RVD Match Score: 3.003	Left Half-Site RVD Score: 4.559	Right Half-Site RVD Score: 7.048
	Genomic Region: Intron	Closest Gene: APOL2
Match Type: R25R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-AGGCC TTCACCACACTCCTAT         GACATTGAATTGCTATTCCTATTGG          ACAGAAGACAGTTCCA AGTCT-3'
	3'-TCCGG AAGTGGTGTGAGGATA         CTGTAACTTAACGATAAGGATAACC          TGTCTTCTGTCAAGGT TCAGA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 022-036626609
Forward Primer: 5'-CTGACTCCAGGCTCTGACAC-3' Tm: 59.508
Reverse Primer: 5'-GCTGGGGCCTTTTTTCTCATCTG-3' Tm: 59.148
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 130, 140
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr22:36626709-36626765

Rank: 157	Homology Score: 5	RVD Match Score: 3.003	Left Half-Site RVD Score: 4.624	Right Half-Site RVD Score: 6.968
	Genomic Region: Intergenic	Closest Gene: ZNF629
Match Type: R19R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GGGAC TGCACCTCCCTCCTAC            TCATTGTGGACAGCAGAGG             AGAAGAGAGAGGAGCA GGAGA-3'
	3'-CCCTG ACGTGGAGGGAGGATG            AGTAACACCTGTCGTCTCC             TCTTCTCTCTCCTCGT CCTCT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 016-030831779
Forward Primer: 5'-CACACACACACACACATGGGC-3' Tm: 59.378
Reverse Primer: 5'-ACTGTCTGGGGAAGGGATGG-3' Tm: 59.508
Expected PCR Product Length: 366
Expected CelI Cleavage Product Sizes: 183, 183
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr16:30831935-30831985

Rank: 158	Homology Score: 5	RVD Match Score: 3.003	Left Half-Site RVD Score: 5.109	Right Half-Site RVD Score: 6.402
	Genomic Region: Exon	Closest Gene: KCNN4
Match Type: R18R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-AGCGG CGCCCCTAAATCCTGC             ACGCACGGCGGGCCCCGC             ACGGGCGCCGGGTGCA GCCCA-3'
	3'-TCGCC GCGGGGATTTAGGACG             TGCGTGCCGCCCGGGGCG             TGCCCGCGGCCCACGT CGGGT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 019-044278490
Forward Primer: 5'-GTAGGAAGCGTTGAGCAGGAC-3' Tm: 59.378
Reverse Primer: 5'-TCCTGACCCCTCCCTTCTTG-3' Tm: 59.508
Expected PCR Product Length: 317
Expected CelI Cleavage Product Sizes: 165, 152
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr19:44278628-44278677

Rank: 159	Homology Score: 2	RVD Match Score: 3.004	Left Half-Site RVD Score: 4.992	Right Half-Site RVD Score: 6.542
	Genomic Region: Exon	Closest Gene: ADAD2
Match Type: R16R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GAACC CACCCCTGACACCTGC              CGTGGCCTGAGCCTCA              ACTGGAGCCTGGGGGA CCCTG-3'
	3'-CTTGG GTGGGGACTGTGGACG              GCACCGGACTCGGAGT              TGACCTCGGACCCCCT GGGAC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 016-084229758
Forward Primer: 5'-TCTGAGCAGGGCCATCCACA-3' Tm: 59.508
Reverse Primer: 5'-TGCATGAGCCCTCCATCACC-3' Tm: 59.508
Expected PCR Product Length: 279
Expected CelI Cleavage Product Sizes: 149, 130
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr16:84229881-84229928

Rank: 160	Homology Score: 2	RVD Match Score: 3.004	Left Half-Site RVD Score: 6.217	Right Half-Site RVD Score: 5.286
	Genomic Region: Exon	Closest Gene: UMODL1
Match Type: R29R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CTCCA TGCACCTCAAATCTCT       CTCTCCTTCCTTTTGTAAGGATGCCAGTC        ATTGGATTTAGGTTCA CCTTA-3'
	3'-GAGGT ACGTGGAGTTTAGAGA       GAGAGGAAGGAAAACATTCCTACGGTCAG        TAACCTAAATCCAAGT GGAAT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 021-043483081
Forward Primer: 5'-TGGAGGCTGCGAGGGAGAAA-3' Tm: 59.508
Reverse Primer: 5'-GCACCCCTGCAAAAGATATGTCC-3' Tm: 59.148
Expected PCR Product Length: 302
Expected CelI Cleavage Product Sizes: 157, 145
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr21:43483206-43483266

Rank: 161	Homology Score: 8	RVD Match Score: 3.006	Left Half-Site RVD Score: 5.848	Right Half-Site RVD Score: 5.649
	Genomic Region: Intergenic	Closest Gene: RGS17
Match Type: R11R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CCTTG TGCAGCTGGCTCCCAT                GATGGCAGCCA                 AATGGAGGCTGGTGCA CTGTG-3'
	3'-GGAAC ACGTCGACCGAGGGTA                CTACCGTCGGT                 TTACCTCCGACCACGT GACAC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 006-153846994
Forward Primer: 5'-GCAAGCTGTCTCATGCTCACTG-3' Tm: 59.258
Reverse Primer: 5'-CAGGAAGGAACTTGTCAGATTCCC-3' Tm: 59.038
Expected PCR Product Length: 366
Expected CelI Cleavage Product Sizes: 229, 137
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr6:153847200-153847242

Rank: 162	Homology Score: 5	RVD Match Score: 3.007	Left Half-Site RVD Score: 7.152	Right Half-Site RVD Score: 4.505
	Genomic Region: Intergenic	Closest Gene: C9orf62
Match Type: R26R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GGAGC TGGAAATGCCTCCTGA         GCTCCAGCCACCCTGCCAATCAGGGA         CCAGGAGGCAGGTGTG GGAGC-3'
	3'-CCTCG ACCTTTACGGAGGACT         CGAGGTCGGTGGGACGGTTAGTCCCT         GGTCCTCCGTCCACAC CCTCG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 009-138280949
Forward Primer: 5'-CGCAGAGGCTCTTTGTGAGCT-3' Tm: 59.378
Reverse Primer: 5'-CTCCAGCTCCTGCATGTCATTC-3' Tm: 59.258
Expected PCR Product Length: 273
Expected CelI Cleavage Product Sizes: 131, 142
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr9:138281049-138281106

Rank: 163	Homology Score: 2	RVD Match Score: 3.007	Left Half-Site RVD Score: 5.053	Right Half-Site RVD Score: 6.497
	Genomic Region: Intron	Closest Gene: ERLIN1
Match Type: R21R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CAGCT CCCACACCACTCCTGT           CCCCAACTTGAACAGCAACAG            TAAGGAGGGAGGAGCA TGGCC-3'
	3'-GTCGA GGGTGTGGTGAGGACA           GGGGTTGAACTTGTCGTTGTC            ATTCCTCCCTCCTCGT ACCGG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 010-101944614
Forward Primer: 5'-CCAATTTCTCCACAAACTGGCCTC-3' Tm: 59.038
Reverse Primer: 5'-GTTTGCCGCTGTAGATGCTGTATG-3' Tm: 59.038
Expected PCR Product Length: 315
Expected CelI Cleavage Product Sizes: 134, 181
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr10:101944720-101944772

Rank: 164	Homology Score: 10	RVD Match Score: 3.007	Left Half-Site RVD Score: 5.285	Right Half-Site RVD Score: 6.24
	Genomic Region: Intron	Closest Gene: SH3RF1
Match Type: R14R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GTTTC TGTACCTCACTTCTGT               TTTCTGTGAATCAG               AGAGGATTTCGGTCCA TGAAT-3'
	3'-CAAAG ACATGGAGTGAAGACA               AAAGACACTTAGTC               TCTCCTAAAGCCAGGT ACTTA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 004-170092193
Forward Primer: 5'-GACCTAAAGGTTTCTCCACACATAGTGAAG-3' Tm: 60.058
Reverse Primer: 5'-GACTGTTCTGAGATCTTGGGTGTG-3' Tm: 59.038
Expected PCR Product Length: 350
Expected CelI Cleavage Product Sizes: 192, 158
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr4:170092360-170092405

Rank: 165	Homology Score: 2	RVD Match Score: 3.008	Left Half-Site RVD Score: 6.245	Right Half-Site RVD Score: 5.286
	Genomic Region: Intron	Closest Gene: PCCA
Match Type: R26R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CCATA TGCCCCTCCCTCATGC         CACACCATTTCTCTACTTAACATCTC         ATATTAGTTTGGTGCA TTTGT-3'
	3'-GGTAT ACGGGGAGGGAGTACG         GTGTGGTAAAGAGATGAATTGTAGAG         TATAATCAAACCACGT AAACA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 013-100940227
Forward Primer: 5'-GTGGTAGACCTTCAGAATTTTATGAGGGAG-3' Tm: 60.058
Reverse Primer: 5'-TCACCACTGCACTCCAGCCT-3' Tm: 59.508
Expected PCR Product Length: 387
Expected CelI Cleavage Product Sizes: 250, 137
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr13:100940446-100940503

Rank: 166	Homology Score: 8	RVD Match Score: 3.008	Left Half-Site RVD Score: 5.703	Right Half-Site RVD Score: 5.81
	Genomic Region: Intron	Closest Gene: NPC1L1
Match Type: R21R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CCCTC TTCACCTCAGCCCTGT           GACATAGCCCCCAGGGCTTCT            GCAGGTGGCAGGTTCA TTCTG-3'
	3'-GGGAG AAGTGGAGTCGGGACA           CTGTATCGGGGGTCCCGAAGA            CGTCCACCGTCCAAGT AAGAC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 007-044570049
Forward Primer: 5'-GGAGATGGATGGTGGTGATAGTTG-3' Tm: 59.038
Reverse Primer: 5'-TTAGGCAGTACTGGCCTGGG-3' Tm: 59.508
Expected PCR Product Length: 434
Expected CelI Cleavage Product Sizes: 294, 140
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr7:44570315-44570367

Rank: 167	Homology Score: 5	RVD Match Score: 3.009	Left Half-Site RVD Score: 6.498	Right Half-Site RVD Score: 5.066
	Genomic Region: Intergenic	Closest Gene: DDX3Y
Match Type: R27R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-ATCCT TTCACCTCAGTCTTCT        GAGTTGCTGGGCTTACAGGTCAGAGCC         ACAGGACCCAGGTGTG TTGTG-3'
	3'-TAGGA AAGTGGAGTCAGAAGA        CTCAACGACCCGAATGTCCAGTCTCGG         TGTCCTGGGTCCACAC AACAC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 00Y-015000077
Forward Primer: 5'-CAGGTGTGTGTCAACAAGCCC-3' Tm: 59.378
Reverse Primer: 5'-CCAGTCTAGGCAACAGAGTGAG-3' Tm: 59.258
Expected PCR Product Length: 272
Expected CelI Cleavage Product Sizes: 130, 142
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chrY:15000176-15000234

Rank: 168	Homology Score: 5	RVD Match Score: 3.009	Left Half-Site RVD Score: 6.686	Right Half-Site RVD Score: 4.904
	Genomic Region: Intron	Closest Gene: ATRNL1
Match Type: R21R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-AACTC TGCACTTATCTACTGA           CAAAAGTCACTGGCAGGAGCC            TCAGGAGTCAGGTAGG AGGGT-3'
	3'-TTGAG ACGTGAATAGATGACT           GTTTTCAGTGACCGTCCTCGG            AGTCCTCAGTCCATCC TCCCA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Warning! Primer pair was generated using suboptimal parameters. 
Primer Index: 010-117176374
Forward Primer: 5'-CAATATCTCAGTATTATCACTTTGAAAACACTTGAGGG-3' Tm: 59.358
Reverse Primer: 5'-CTTGGGGATCAGGGCCATTTC-3' Tm: 59.378
Expected PCR Product Length: 289
Expected CelI Cleavage Product Sizes: 135, 154
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr10:117176481-117176533

Rank: 169	Homology Score: 2	RVD Match Score: 3.009	Left Half-Site RVD Score: 5.053	Right Half-Site RVD Score: 6.519
	Genomic Region: Intron	Closest Gene: ATP2B3
Match Type: R25R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CTCTC CCCACCTGAATCCTCC         CAAGTCCTGTCCCTTTTGAGAAGCT          CCTGGAGTCATGAGCG CTCTA-3'
	3'-GAGAG GGGTGGACTTAGGAGG         GTTCAGGACAGGGAAAACTCTTCGA          GGACCTCAGTACTCGC GAGAT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 00X-152817806
Forward Primer: 5'-TCACCAGCCTCCCATGCAGT-3' Tm: 59.508
Reverse Primer: 5'-GGCAGCTCCACCACAGACTT-3' Tm: 59.508
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 134, 136
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chrX:152817910-152817966

Rank: 170	Homology Score: 5	RVD Match Score: 3.01	Left Half-Site RVD Score: 5.044	Right Half-Site RVD Score: 6.534
	Genomic Region: Exon	Closest Gene: TRIM67
Match Type: R18R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TACCC TGAACCTAACCCCTTT             GAGGGAGCAGCATCCAGG             TCAGGAGAGAAGTGGA AAGTG-3'
	3'-ATGGG ACTTGGATTGGGGAAA             CTCCCTCGTCGTAGGTCC             AGTCCTCTCTTCACCT TTCAC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 001-231353429
Forward Primer: 5'-AAGGCTGTGGAGCCTCCACCAT-3' Tm: 61.288
Reverse Primer: 5'-AATCCCAGCCCCCAAGCTCA-3' Tm: 59.508
Expected PCR Product Length: 279
Expected CelI Cleavage Product Sizes: 131, 148
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr1:231353533-231353582

Rank: 171	Homology Score: 2	RVD Match Score: 3.01	Left Half-Site RVD Score: 5.374	Right Half-Site RVD Score: 6.173
	Genomic Region: Intron	Closest Gene: CD99P1
Match Type: R27R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CTTTC CACCCCTGACTCTTGC        TCTCATCTTTTACTCTTTGTGTCTGAG         AGAAGAGTGATGTGAA AATGA-3'
	3'-GAAAG GTGGGGACTGAGAACG        AGAGTAGAAAATGAGAAACACAGACTC         TCTTCTCACTACACTT TTACT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 00Y-002494311
Forward Primer: 5'-ACAGGAGGGAATCCTGTGTTGG-3' Tm: 59.258
Reverse Primer: 5'-GACCTGGAAGCAGATGGAGAAAAC-3' Tm: 59.038
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 137, 133
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chrY:2494417-2494475

Rank: 172	Homology Score: 2	RVD Match Score: 3.01	Left Half-Site RVD Score: 5.374	Right Half-Site RVD Score: 6.173
	Genomic Region: Intron	Closest Gene: CD99P1
Match Type: R27R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CTTTC CACCCCTGACTCTTGC        TCTCATCTTTTACTCTTTGTGTCTGAG         AGAAGAGTGATGTGAA AATGA-3'
	3'-GAAAG GTGGGGACTGAGAACG        AGAGTAGAAAATGAGAAACACAGACTC         TCTTCTCACTACACTT TTACT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 00X-002544311
Forward Primer: 5'-ACAGGAGGGAATCCTGTGTTGG-3' Tm: 59.258
Reverse Primer: 5'-GACCTGGAAGCAGATGGAGAAAAC-3' Tm: 59.038
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 137, 133
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chrX:2544417-2544475

Rank: 173	Homology Score: 5	RVD Match Score: 3.01	Left Half-Site RVD Score: 6.229	Right Half-Site RVD Score: 5.322
	Genomic Region: Intron	Closest Gene: PRKG1
Match Type: R10R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GGATC TGTACCTTCCTCATAT                 GAACATAATG                 ATAGTAGTCATGTTCA TTAAG-3'
	3'-CCTAG ACATGGAAGGAGTATA                 CTTGTATTAC                 TATCATCAGTACAAGT AATTC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 010-052784032
Forward Primer: 5'-ACTGAGGCCCAAATCATAGCCAATGATGGA-3' Tm: 61.548
Reverse Primer: 5'-AGGCCTCCACAGCCATGCTT-3' Tm: 59.508
Expected PCR Product Length: 416
Expected CelI Cleavage Product Sizes: 131, 285
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr10:52784140-52784181

Rank: 174	Homology Score: 5	RVD Match Score: 3.012	Left Half-Site RVD Score: 5.648	Right Half-Site RVD Score: 5.899
	Genomic Region: Intergenic	Closest Gene: SORCS1
Match Type: R15R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TAATA TGCACCAGACTTCTAG              TATTAAATATTGGGT               AAATGAGTAATGTGTA CCCAT-3'
	3'-ATTAT ACGTGGTCTGAAGATC              ATAATTTATAACCCA               TTTACTCATTACACAT GGGTA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 010-107873570
Forward Primer: 5'-GACACCTTCTGCTAAGCACACATC-3' Tm: 59.038
Reverse Primer: 5'-GAGCTCATTCAAAGCCCCAGTAG-3' Tm: 59.148
Expected PCR Product Length: 358
Expected CelI Cleavage Product Sizes: 183, 175
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr10:107873728-107873774

Rank: 175	Homology Score: 2	RVD Match Score: 3.012	Left Half-Site RVD Score: 6.493	Right Half-Site RVD Score: 5.096
	Genomic Region: Intron	Closest Gene: GLS
Match Type: R11R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CTCTA TGCATTTACCTCCTTT                CCCTAAAATGT                 ATCGGATTGAGGTGTA GGAGT-3'
	3'-GAGAT ACGTAAATGGAGGAAA                GGGATTTTACA                 TAGCCTAACTCCACAT CCTCA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 002-191816679
Forward Primer: 5'-CCAACAGTTTGGGAGGCCAAG-3' Tm: 59.378
Reverse Primer: 5'-CTGCCCAGAGTCCAGAACAGT-3' Tm: 59.378
Expected PCR Product Length: 275
Expected CelI Cleavage Product Sizes: 130, 145
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr2:191816786-191816828

Rank: 176	Homology Score: 2	RVD Match Score: 3.012	Left Half-Site RVD Score: 5.816	Right Half-Site RVD Score: 5.732
	Genomic Region: Intron	Closest Gene: NCALD
Match Type: R30R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-ACTGT CACATGTGCCTCCTGT       GAGCCGAACTGCCAGCCTTTCCATTCAGGA       AGAGAAGTGTGGTGGA AAACC-3'
	3'-TGACA GTGTACACGGAGGACA       CTCGGCTTGACGGTCGGAAAGGTAAGTCCT       TCTCTTCACACCACCT TTTGG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 008-102790216
Forward Primer: 5'-GGGAGAAGGAACAGTCAAGGC-3' Tm: 59.378
Reverse Primer: 5'-TGAAGGGCTGGGTTGACTGG-3' Tm: 59.508
Expected PCR Product Length: 353
Expected CelI Cleavage Product Sizes: 219, 134
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr8:102790402-102790463

Rank: 177	Homology Score: 5	RVD Match Score: 3.013	Left Half-Site RVD Score: 7.591	Right Half-Site RVD Score: 4.21
	Genomic Region: Intron	Closest Gene: LOC339298
Match Type: R14R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GATAA TTGACCTGAGGCCTGA               AGTCGCCAGGAAGC               ACAGGAGGCTGGTGTG CCCGA-3'
	3'-CTATT AACTGGACTCCGGACT               TCAGCGGTCCTTCG               TGTCCTCCGACCACAC GGGCT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 018-073844625
Forward Primer: 5'-CCGCAATGGGATAAGAGGCAAC-3' Tm: 59.258
Reverse Primer: 5'-GCGCTCCTGTTAAAAATCCGGAGT-3' Tm: 59.038
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 131, 139
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr18:73844731-73844776

Rank: 178	Homology Score: 5	RVD Match Score: 3.013	Left Half-Site RVD Score: 6.084	Right Half-Site RVD Score: 5.479
	Genomic Region: Intergenic	Closest Gene: HNMT
Match Type: R20R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CTCAA TGGACTTTACTCCTCT            GAGGAAGGTTAGCAACGGTT            ATAGGAGTCAGTAGAG GCACC-3'
	3'-GAGTT ACCTGAAATGAGGAGA            CTCCTTCCAATCGTTGCCAA            TATCCTCAGTCATCTC CGTGG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 002-138683178
Forward Primer: 5'-CTGTCCCCTCTCCTTGTACTTTTC-3' Tm: 59.038
Reverse Primer: 5'-GGGAGCCTTGGACAATAAGCTG-3' Tm: 59.258
Expected PCR Product Length: 336
Expected CelI Cleavage Product Sizes: 167, 169
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr2:138683317-138683368

Rank: 179	Homology Score: 10	RVD Match Score: 3.013	Left Half-Site RVD Score: 4.802	Right Half-Site RVD Score: 6.843
	Genomic Region: Intergenic	Closest Gene: DKFZp686K1684
Match Type: R11R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GAACT TTCACCTGACTCCTTA                TGCTTAGCTCA                 AACGGGGGCAGGTTCA AGCAG-3'
	3'-CTTGA AAGTGGACTGAGGAAT                ACGAATCGAGT                 TTGCCCCCGTCCAAGT TCGTC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 011-031965225
Forward Primer: 5'-CCAGCCTGAGACAACCATCTTTAC-3' Tm: 59.038
Reverse Primer: 5'-GAAGGGCCATAACTTGGGCATTAG-3' Tm: 59.038
Expected PCR Product Length: 355
Expected CelI Cleavage Product Sizes: 192, 163
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr11:31965394-31965436

Rank: 180	Homology Score: 5	RVD Match Score: 3.014	Left Half-Site RVD Score: 5.82	Right Half-Site RVD Score: 5.739
	Genomic Region: Intron	Closest Gene: DPP6
Match Type: R17R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TTTCC CCCACATTACTGCTGT             GAAACATTTGTGAGAAA              TCAGGTGACAGGTGGA TCTAG-3'
	3'-AAAGG GGGTGTAATGACGACA             CTTTGTAAACACTCTTT              AGTCCACTGTCCACCT AGATC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Warning! Primer pair was generated using suboptimal parameters. 
Primer Index: 007-154052611
Forward Primer: 5'-GAATATCTTTTTGAGTATGGTGTGAGGTAGGG-3' Tm: 59.848
Reverse Primer: 5'-GAGTATGTGTGTGTAATTTGACAAGCCGATTC-3' Tm: 59.848
Expected PCR Product Length: 351
Expected CelI Cleavage Product Sizes: 130, 221
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr7:154052715-154052763

Rank: 181	Homology Score: 8	RVD Match Score: 3.014	Left Half-Site RVD Score: 5.923	Right Half-Site RVD Score: 5.642
	Genomic Region: Intron	Closest Gene: VASH2
Match Type: R12R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CATTT TTCACCAGACTCCCGG                CTCTTCAGCCTG                AGAGGAATATGGTGCA GAGAA-3'
	3'-GTAAA AAGTGGTCTGAGGGCC                GAGAAGTCGGAC                TCTCCTTATACCACGT CTCTT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 001-213140398
Forward Primer: 5'-ATTCTAAGAGTGGCGTGTGGAGG-3' Tm: 59.148
Reverse Primer: 5'-GTTGTGGGTGATTCCAATGGCATG-3' Tm: 59.038
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 134, 136
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr1:213140508-213140551

Rank: 182	Homology Score: 10	RVD Match Score: 3.016	Left Half-Site RVD Score: 5.293	Right Half-Site RVD Score: 6.305
	Genomic Region: Intron	Closest Gene: C1orf198
Match Type: R20R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GGGCT TGCCCTTGACACCTGT            CTCAGGCCCAGTTTGGCTAC            ATGTGAGACTGGTGCA GAGTG-3'
	3'-CCCGA ACGGGAACTGTGGACA            GAGTCCGGGTCAAACCGATG            TACACTCTGACCACGT CTCAC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 001-230976433
Forward Primer: 5'-GCGCCAAGGTCTTCTTGAGC-3' Tm: 59.508
Reverse Primer: 5'-GGAGAGTAGGAGACAGCACATG-3' Tm: 59.258
Expected PCR Product Length: 312
Expected CelI Cleavage Product Sizes: 136, 176
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr1:230976541-230976592

Rank: 183	Homology Score: 2	RVD Match Score: 3.017	Left Half-Site RVD Score: 5.088	Right Half-Site RVD Score: 6.541
	Genomic Region: Intergenic	Closest Gene: SSTR1
Match Type: R22R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-AGTAA CACAACTGACTCCAGA           TGGTCATACATGCTAACTTACA           GGAGGAGTAATGTACA TGTGT-3'
	3'-TCATT GTGTTGACTGAGGTCT           ACCAGTATGTACGATTGAATGT           CCTCCTCATTACATGT ACACA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 014-038458927
Forward Primer: 5'-CGTTGCAATTTCCCATCCGCATGT-3' Tm: 59.038
Reverse Primer: 5'-CCTGGGGTTTTCTTTGTGGAAGGA-3' Tm: 59.038
Expected PCR Product Length: 401
Expected CelI Cleavage Product Sizes: 219, 182
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr14:38459117-38459170

Rank: 184	Homology Score: 5	RVD Match Score: 3.019	Left Half-Site RVD Score: 5.747	Right Half-Site RVD Score: 5.848
	Genomic Region: Intron	Closest Gene: SUMF1
Match Type: R19R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GACTT TACTCCTCACTCCTCG            TTTTGTTCACCCCAGAAGC             ACAGCAGTCTGGAGTA AAGTC-3'
	3'-CTGAA ATGAGGAGTGAGGAGC            AAAACAAGTGGGGTCTTCG             TGTCGTCAGACCTCAT TTCAG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 003-004440767
Forward Primer: 5'-TATGAGAACGAGAGGGGGCG-3' Tm: 59.508
Reverse Primer: 5'-CAAGCCCTTCCCCACTGTAG-3' Tm: 59.508
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 136, 134
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr3:4440876-4440926

Rank: 185	Homology Score: 5	RVD Match Score: 3.019	Left Half-Site RVD Score: 5.326	Right Half-Site RVD Score: 6.291
	Genomic Region: Intergenic	Closest Gene: MID1
Match Type: R28R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CATCT TCCACCTTTCTCCTCT        CTGGCGGCCTGCAGCTTCTGCCTCCTCC        ACAGCAGCCAGCTGTG TGAAG-3'
	3'-GTAGA AGGTGGAAAGAGGAGA        GACCGCCGGACGTCGAAGACGGAGGAGG        TGTCGTCGGTCGACAC ACTTC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 00X-010361133
Forward Primer: 5'-CCGGATCTTCAACAAGCCCC-3' Tm: 59.508
Reverse Primer: 5'-CATCCTACATGAGAAAGAGCCAGC-3' Tm: 59.038
Expected PCR Product Length: 391
Expected CelI Cleavage Product Sizes: 249, 142
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chrX:10361350-10361409

Rank: 186	Homology Score: 2	RVD Match Score: 3.02	Left Half-Site RVD Score: 5.815	Right Half-Site RVD Score: 5.788
	Genomic Region: Intron	Closest Gene: PTPRD
Match Type: R25R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-AGTCA TACACTTAATCCCTGT         GCCATAATTCAATAAATTGTGGAGG          ATGGGAAGCAGGTGGA GAAAT-3'
	3'-TCAGT ATGTGAATTAGGGACA         CGGTATTAAGTTATTTAACACCTCC          TACCCTTCGTCCACCT CTTTA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 009-009921803
Forward Primer: 5'-CAGCTGATATTATGTGTCCCACACTGTTC-3' Tm: 60.178
Reverse Primer: 5'-GAGTTGCTCACAGCTTCTCTGAGT-3' Tm: 59.038
Expected PCR Product Length: 338
Expected CelI Cleavage Product Sizes: 207, 131
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr9:9921980-9922036

Rank: 187	Homology Score: 8	RVD Match Score: 3.02	Left Half-Site RVD Score: 5.428	Right Half-Site RVD Score: 6.19
	Genomic Region: Intron	Closest Gene: MGMT
Match Type: R30R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-ACACA TCCACGTAACTCTTGT       CCTTAGCAGTAGTGAGGGGCTTCTGCAGGC       AGAGGAGTCGGCTCCA CGGTC-3'
	3'-TGTGT AGGTGCATTGAGAACA       GGAATCGTCATCACTCCCCGAAGACGTCCG       TCTCCTCAGCCGAGGT GCCAG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 010-131523914
Forward Primer: 5'-CTCGGAGGTCTGTCCATGCT-3' Tm: 59.508
Reverse Primer: 5'-GAGCTAGAAGGGGCTGGTTG-3' Tm: 59.508
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 135, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr10:131524016-131524077

Rank: 188	Homology Score: 10	RVD Match Score: 3.02	Left Half-Site RVD Score: 6.224	Right Half-Site RVD Score: 5.399
	Genomic Region: Intron	Closest Gene: TMEM72-AS1
Match Type: R29R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GGGGG TGCCCATGACTCCCCC       TTAGGTTTAACAATGCACTTGAATGACTC        ACAGGACTCAGGAGGA CACTC-3'
	3'-CCCCC ACGGGTACTGAGGGGG       AATCCAAATTGTTACGTGAACTTACTGAG        TGTCCTGAGTCCTCCT GTGAG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 010-045378787
Forward Primer: 5'-GGTGAGGCTCCATCCCACAA-3' Tm: 59.508
Reverse Primer: 5'-ACCCAGAGAGGGTGTGAGAG-3' Tm: 59.508
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 133, 137
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr10:45378888-45378948

Rank: 189	Homology Score: 5	RVD Match Score: 3.022	Left Half-Site RVD Score: 6.496	Right Half-Site RVD Score: 5.163
	Genomic Region: Intron	Closest Gene: CABIN1
Match Type: R25R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TGTGT TGCTTCTCACTCCTAG         GTGCTTCATTTTCTAGTTATTCTTG          ATAGGAGTGACTTGCA GTCTA-3'
	3'-ACACA ACGAAGAGTGAGGATC         CACGAAGTAAAAGATCAATAAGAAC          TATCCTCACTGAACGT CAGAT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 022-024444126
Forward Primer: 5'-TGCTCTCTCCTCCCAAGCAC-3' Tm: 59.508
Reverse Primer: 5'-GGTCTCACCAGTGAACACACAG-3' Tm: 59.258
Expected PCR Product Length: 272
Expected CelI Cleavage Product Sizes: 130, 142
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr22:24444226-24444282

Rank: 190	Homology Score: 10	RVD Match Score: 3.022	Left Half-Site RVD Score: 7.259	Right Half-Site RVD Score: 4.527
	Genomic Region: Intergenic	Closest Gene: GRXCR1
Match Type: R25R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TTGTA TCCACCTGGCAGCTGG         TATCATGTAGCACCGCTTATCTTGC          AAAAGAGTTAGGTGCA TGGGT-3'
	3'-AACAT AGGTGGACCGTCGACC         ATAGTACATCGTGGCGAATAGAACG          TTTTCTCAATCCACGT ACCCA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 004-043460367
Forward Primer: 5'-GCTAAGAGCCTCTTGGGCTTTTG-3' Tm: 59.148
Reverse Primer: 5'-AGCAGAGGCAGAAAGGACCAG-3' Tm: 59.378
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 136, 134
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr4:43460473-43460529

Rank: 191	Homology Score: 5	RVD Match Score: 3.023	Left Half-Site RVD Score: 7.565	Right Half-Site RVD Score: 4.291
	Genomic Region: Intergenic	Closest Gene: GPR20
Match Type: R17R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-AGAAG TGTGCCGGACACCTGA             GGAAGGTCCTTCTGCAG              ATGGGAGGCAGGTGTA TCCGC-3'
	3'-TCTTC ACACGGCCTGTGGACT             CCTTCCAGGAAGACGTC              TACCCTCCGTCCACAT AGGCG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 008-142398434
Forward Primer: 5'-GCCTGAGATAGGGACATTGGTG-3' Tm: 59.258
Reverse Primer: 5'-CCCATTCTGAGCGAGCTTGGA-3' Tm: 59.378
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 135, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr8:142398543-142398591

Rank: 192	Homology Score: 10	RVD Match Score: 3.023	Left Half-Site RVD Score: 5.831	Right Half-Site RVD Score: 5.797
	Genomic Region: Intron	Closest Gene: DENND4C
Match Type: R25R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GAGTC TGCAGATGACTTCTGT         TTATTTCCTATAGTACAAAATTAGT          ATGGGAGTGCTGTGCA CAGTG-3'
	3'-CTCAG ACGTCTACTGAAGACA         AATAAAGGATATCATGTTTTAATCA          TACCCTCACGACACGT GTCAC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 009-019336805
Forward Primer: 5'-GTCCATTGATAATCACTCTAGCACAGGTAC-3' Tm: 60.058
Reverse Primer: 5'-GTGTGACTTCCTTCCTTCCCTC-3' Tm: 59.258
Expected PCR Product Length: 422
Expected CelI Cleavage Product Sizes: 199, 223
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr9:19336974-19337030

Rank: 193	Homology Score: 5	RVD Match Score: 3.024	Left Half-Site RVD Score: 5.489	Right Half-Site RVD Score: 6.157
	Genomic Region: Intergenic	Closest Gene: SLC6A19
Match Type: R18R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GGGGA CGGACGTCACTCCTAT             TCCATGTCTTGTCTTTCC             ACAGGACTCACGTACG GACTC-3'
	3'-CCCCT GCCTGCAGTGAGGATA             AGGTACAGAACAGAAAGG             TGTCCTGAGTGCATGC CTGAG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 005-001179126
Forward Primer: 5'-AAGCTGCACGCAGCCCTCAT-3' Tm: 59.508
Reverse Primer: 5'-GTCTTGAGGCCCCAGGGAAA-3' Tm: 59.508
Expected PCR Product Length: 403
Expected CelI Cleavage Product Sizes: 134, 269
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr5:1179233-1179282

Rank: 194	Homology Score: 10	RVD Match Score: 3.026	Left Half-Site RVD Score: 5.505	Right Half-Site RVD Score: 6.152
	Genomic Region: Intergenic	Closest Gene: COG2
Match Type: R28R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TTATT TGAACATGACTTCTGT        GCATTCCATTGCACCATGTGGTGGGGAG        AAAGGTGTCTGGTCTA TTTTT-3'
	3'-AATAA ACTTGTACTGAAGACA        CGTAAGGTAACGTGGTACACCACCCCTC        TTTCCACAGACCAGAT AAAAA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 001-230749531
Forward Primer: 5'-CAAAGATGCAAAGGCTGATTTAGAGCACC-3' Tm: 60.178
Reverse Primer: 5'-CCCCAAATTTAGCAATACTGTCTGCTGGA-3' Tm: 60.178
Expected PCR Product Length: 441
Expected CelI Cleavage Product Sizes: 131, 310
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr1:230749630-230749689

Rank: 195	Homology Score: 2	RVD Match Score: 3.026	Left Half-Site RVD Score: 5.339	Right Half-Site RVD Score: 6.334
	Genomic Region: Exon	Closest Gene: PLA2G4A
Match Type: R11R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TTTGC CACACAGGACTCCTTT                GATGATGATGA                 ACTGGATGCAGCTGTA GCAGG-3'
	3'-AAACG GTGTGTCCTGAGGAAA                CTACTACTACT                 TGACCTACGTCGACAT CGTCC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 001-186925307
Forward Primer: 5'-GGCCTTGGTGAGTGATTCAGC-3' Tm: 59.378
Reverse Primer: 5'-CCCTAGTCATTTGAAGCCCTTCAC-3' Tm: 59.038
Expected PCR Product Length: 322
Expected CelI Cleavage Product Sizes: 146, 176
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr1:186925430-186925472

Rank: 196	Homology Score: 2	RVD Match Score: 3.026	Left Half-Site RVD Score: 6.137	Right Half-Site RVD Score: 5.523
	Genomic Region: Intron	Closest Gene: KRTDAP
Match Type: R11R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-AGTTG CGCTCCTCAGTCCTTT                CAGCTAGAGGG                 AGAGGGGTCAGGGTTA GATGG-3'
	3'-TCAAC GCGAGGAGTCAGGAAA                GTCGATCTCCC                 TCTCCCCAGTCCCAAT CTACC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 019-035978213
Forward Primer: 5'-CAGATACAGGAGCTGTTGGATGG-3' Tm: 59.148
Reverse Primer: 5'-CTGAAGGGAGGTCTTAGCTACTAC-3' Tm: 59.038
Expected PCR Product Length: 291
Expected CelI Cleavage Product Sizes: 160, 131
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr19:35978350-35978392

Rank: 197	Homology Score: 5	RVD Match Score: 3.027	Left Half-Site RVD Score: 8.515	Right Half-Site RVD Score: 5.743
	Genomic Region: Intron	Closest Gene: OR8U8
Match Type: L17R
	           Left Nuclease
	         TCACCTTGCCCCACAGGGCAGT
	5'-ATGTC TCGCCTCCCGCCACAGGGCGGT       TTTTCTCCTATCTCAGA              ATAGAAGAAAGGTGCA ATCAG-3'
	3'-TACAG AGCGGAGGGCGGTGTCCCGCCA       AAAAGAGGATAGAGTCT              TATCTTCTTTCCACGT TAGTC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 011-056386094
Forward Primer: 5'-GGGAGGTACTATGCCTGGATG-3' Tm: 59.378
Reverse Primer: 5'-GCTCGGGAGGATTAGTAAAAGAGG-3' Tm: 59.038
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 136, 134
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr11:56386201-56386255

Rank: 198	Homology Score: 2	RVD Match Score: 3.027	Left Half-Site RVD Score: 5.406	Right Half-Site RVD Score: 6.273
	Genomic Region: Intron	Closest Gene: RBKS
Match Type: R17R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TGCAC TGCACCCACCTCCTCA             CAGAGCTGTGCGAAGAG              ACGTGAGTTAAGGGCA TGTAC-3'
	3'-ACGTG ACGTGGGTGGAGGAGT             GTCTCGACACGCTTCTC              TGCACTCAATTCCCGT ACATG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 002-028093265
Forward Primer: 5'-GCTGCCAGTGGTGACTGTTTTTC-3' Tm: 59.148
Reverse Primer: 5'-GTGAGTCTACACACCATGTCATCC-3' Tm: 59.038
Expected PCR Product Length: 292
Expected CelI Cleavage Product Sizes: 154, 138
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr2:28093393-28093441

Rank: 199	Homology Score: 2	RVD Match Score: 3.028	Left Half-Site RVD Score: 5.495	Right Half-Site RVD Score: 6.182
	Genomic Region: Intron	Closest Gene: LOC339975
Match Type: R27R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-ATTTA TACACCTGAGCCCTAA        AAGTCTGGGGTCATTCAGTGTTTCTGA         AGGGCAGGCAGGTGGA TATCA-3'
	3'-TAAAT ATGTGGACTCGGGATT        TTCAGACCCCAGTAAGTCACAAAGACT         TCCCGTCCGTCCACCT ATAGT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 004-188278629
Forward Primer: 5'-CCTTTCCCTTACCCTGTTTGTTCC-3' Tm: 59.038
Reverse Primer: 5'-GTGTCCTAGGAACACTGCTCTG-3' Tm: 59.258
Expected PCR Product Length: 404
Expected CelI Cleavage Product Sizes: 260, 144
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr4:188278858-188278916

Rank: 200	Homology Score: 5	RVD Match Score: 3.028	Left Half-Site RVD Score: 6.636	Right Half-Site RVD Score: 5.086
	Genomic Region: Intron	Closest Gene: DIS3L2
Match Type: R20R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-ATTGG TGCTCCTGAAGACTGT            CCCTCCCAAGTCTTGCCTTG            ACAGGAGCTAGATGTG GGGTC-3'
	3'-TAACC ACGAGGACTTCTGACA            GGGAGGGTTCAGAACGGAAC            TGTCCTCGATCTACAC CCCAG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 002-232990297
Forward Primer: 5'-GGTAATATAGGCGAGCATGCTGTG-3' Tm: 59.038
Reverse Primer: 5'-GAAAACACAAGTCCTCACAGGGG-3' Tm: 59.148
Expected PCR Product Length: 274
Expected CelI Cleavage Product Sizes: 144, 130
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr2:232990413-232990464

Plate format Oligo Order Form
Well
Name
Sequence
5' mods
Internal
Notes
Primer Plate #1
A1
011-005248069F
GTGCCTATCAGAAACCCAAGAGTC
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
A2
011-005248069R
CAATCTACTCCCAGGAGCAGG
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
B1
011-005255477F
GTCAGTGCCTATCAGAAACCCAAG
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
B2
011-005255477R
TCACTGGAGCAGGGAGGACA
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
C1
009-094951264F
ATCCAGCCCTGGGACTTTACTTC
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
C2
009-094951264R
TGTGAGTCTCAGAACCAAGGCTGT
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
D1
00X-034076224F
AGTATTGCCCTTTCTGCTAGGGAC
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
D2
00X-034076224R
CACTGCACACTTCTGTGGCTC
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
E1
003-126892267F
ACCCAGACCCAGACATCCAC
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
E2
003-126892267R
CTATGTGCAGCTGGAGAAACTGAG
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
F1
003-027142836F
AGGACAGGAAGGAAGAGCCC
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
F2
003-027142836R
GTGTGGATTGGCAACCTCTTCATG
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
G1
006-123066634F
GTAGTGGGATCGTGCCTGTG
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
G2
006-123066634R
TCAGTACACTTGGACCCGGC
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
H1
016-083864892F
GGAAACTGTGGCCCAGAGAG
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
H2
016-083864892R
CTCAGCATCCGTTCCTGTTCTTC
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
A3
022-045287459F
CAAAGGCTCTGCCTGCAGGA
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
A4
022-045287459R
CAGGACTAAGGCCGCTCTTC
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
B3
008-144606723F
CTAGCAGCAGGCAAGTCGCA
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
B4
008-144606723R
ACACAGAGCATGGGCTTGGG
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
C3
006-110284189F
GCAATGTTGCTCCAAGAGGCC
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
C4
006-110284189R
GAACTTATTCCTCCCACCTAGCTG
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
D3
012-072604585F
GTTGAGAGTTGACACCAACTTCTTTTCTTTTTCC
S2_S5_NN_Ranking-RVDs_5TC	Warning! Primer pair was generated using suboptimal parameters. 
D4
012-072604585R
CCACTCCTAGTGTGGAACCTC
S2_S5_NN_Ranking-RVDs_5TC	Warning! Primer pair was generated using suboptimal parameters. 
E3
010-029082810F
CCTCTGAGCCCCATTCTCCT
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
E4
010-029082810R
TAGAGATGGGGTCTCACCTTGTTG
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
F3
003-058640715F
CATATGAGGAAACTGAGAGGCGG
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
F4
003-058640715R
CCTCGAGGAGGGCTATTCTAGA
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
G3
00Y-018946879F
ATACACTTGGACTCGGCTTCCC
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
G4
00Y-018946879R
GTGAATCTCAGGTAGTGTACCTGC
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
H3
dom-000216374F
TCTCCTTTCTGTACCTCAGAGACC
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
H4
dom-000216374R
CCCACCCTGGTACAGCAAATG
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
A5
005-059619945F
CCAAGACTTAGCTTAGCACCCAAC
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
A6
005-059619945R
CATGCCTGGAGTTAGCCTCTCT
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
B5
014-077327823F
CCAACAGCTGGTAAGGATGTCAAG
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
B6
014-077327823R
CTGTGGAAACACCAAAGCAGGG
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
C5
005-177943719F
AAGCTTTCTGCCTCCTCAGGCC
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
C6
005-177943719R
CACCTCAGTGACAGGCGCTT
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
D5
015-082621505F
TGGCTTTCACGGGTCTGTGC
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
D6
015-082621505R
TAGACATGCAGCCTGGAGCC
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
E5
015-082918832F
TGGCTTTCACGGGTCTGTGC
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
E6
015-082918832R
TAGACATGCAGCCTGGAGCC
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
F5
015-085803797F
TAGACATGCAGCCTGGAGCC
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
F6
015-085803797R
TGGCTTTCACGGGTCTGTGC
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
G5
005-111863094F
AGTACACTTGGACCCAGCTTACC
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
G6
005-111863094R
TGAATCCCAGGTAGCGTACCTGC
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
H5
012-040234817F
CCTTACAATTCACGGCTCTCAGAC
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
H6
012-040234817R
CCACTGGAGTTGACAGATTTGGC
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
A7
008-015147764F
CCCCACTTCATCTGCTAAACTTCC
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
A8
008-015147764R
GAGGGGGAAAATGTGGGAAGTTCT
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
B7
011-002239013F
AGCTGTCCAGCCTGCAGCAT
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
B8
011-002239013R
TCATATTCCTGGGTGGTCACTCC
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
C7
00X-079108050F
GGTGTCAGTAGCAGATAGGGATG
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
C8
00X-079108050R
CTGTACCCAACCCACTTTATGGC
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
D7
002-011376315F
CCCCTCATGGAGAACACAATACAAAAGAAAC
S2_S5_NN_Ranking-RVDs_5TC	Warning! Primer pair was generated using suboptimal parameters. 
D8
002-011376315R
GAGAGAAGAGTTACGCGTCTTCC
S2_S5_NN_Ranking-RVDs_5TC	Warning! Primer pair was generated using suboptimal parameters. 
E7
016-071012605F
CTCCTAGCTTCCTGATTCTTTGCC
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
E8
016-071012605R
CCCACCCTGGTACAGCAAATG
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
F7
215-000126245F
GTCCCCTCTCCTTGCACTTTTC
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
F8
215-000126245R
CCTTGGGAACCTTGGACAATAAGC
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
G7
021-015154271F
GTCCCCTCTCCTTGCACTTTTC
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
G8
021-015154271R
CCTTGGGAACCTTGGACAATAAGC
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
H7
019-045727578F
GAGAAGGGGAGTGTTCCACTG
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
H8
019-045727578R
GGATAACAGAGGGCCATCAGC
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
A9
012-101038644F
CTGTGCTCCCCTTTCTGAATAGTG
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
A10
012-101038644R
TACACTTGGACCCGACTTCCC
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
B9
010-095287016F
GAGCTGCTAAAATTGCTTGCTGCC
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
B10
010-095287016R
GCACCCAGCCTGTTCAGCAA
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
C9
No PrimersF
No Primers
S2_S5_NN_Ranking-RVDs_5TC	No Primers
C10
No PrimersR
No Primers
S2_S5_NN_Ranking-RVDs_5TC	No Primers
D9
005-078702362F
GAAAGGATCCTGGATGAGGGG
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
D10
005-078702362R
GTAGGAGTTTCCTGCCTCTGTC
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
E9
014-077327606F
GCCTGGTATAGAACAAGCACCTG
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
E10
014-077327606R
CCTCCTGTGATTGCCAGAGC
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
F9
010-133959783F
TTTCTCTCGGCACCTCCCAC
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
F10
010-133959783R
GTCCCTGCCCATGGACAAGT
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
G9
009-117519299F
CAAAAGGACCTGCATCTAAGCACC
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
G10
009-117519299R
ATTGTTCTTCCCTGGCCACCC
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
H9
009-134936820F
GCTGGTAAGTGAAGTTGATGTGGC
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
H10
009-134936820R
CCAGGAGCAGCTGCGATGAA
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
A11
001-213135498F
TGGTTTGGTCCCTTTCTTCTCCG
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
A12
001-213135498R
AAGCCAGTCAATGTGCCTGGG
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
B11
006-109827303F
CTGGGGATTCGGTTTCAGCATATG
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
B12
006-109827303R
CAGCAAATGGTGGGTATGGAATGAAGTC
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
C11
002-072161892F
GGCTGCTGTCTTTGATGATGCAAC
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
C12
002-072161892R
GCCCCTGGCAATGAAGGCAA
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
D11
007-047858063F
CCACTTTCGGGGAAATGGTATCAC
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
D12
007-047858063R
GCAAGCGTTCTATCCAAGTGCC
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
E11
003-054731501F
CCTACAGTTTCCAAGGACAAGCAG
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
E12
003-054731501R
CCCAGCAGTAGCTTTAGCATCC
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
F11
012-059855477F
GAGAAAGGATTGAGTATTTTTCTCAGGACTTGG
S2_S5_NN_Ranking-RVDs_5TC	Warning! Primer pair was generated using suboptimal parameters. 
F12
012-059855477R
CAGAATTTCAGAGGCCCTCCATC
S2_S5_NN_Ranking-RVDs_5TC	Warning! Primer pair was generated using suboptimal parameters. 
G11
004-002790263F
ATGCAGAGATGGCCCAGAGC
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
G12
004-002790263R
TGAGTAGGGCCAAGGCCACA
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
H11
002-130879063F
AAGACGCCCACCGAGCCCA
S2_S5_NN_Ranking-RVDs_5TC	Warning! Primer pair was generated using suboptimal parameters. 
H12
002-130879063R
GCTAATGGGTGCAAAGTTTCTTTTAAGGAGCATA
S2_S5_NN_Ranking-RVDs_5TC	Warning! Primer pair was generated using suboptimal parameters. 
Primer Plate #2
A1
002-131974522F
GCTAATGGGTGCAAAGTTTCTTTTAAGGAGCATA
S2_S5_NN_Ranking-RVDs_5TC	Warning! Primer pair was generated using suboptimal parameters. 
A2
002-131974522R
AAGACGCCCACCGAGCCCA
S2_S5_NN_Ranking-RVDs_5TC	Warning! Primer pair was generated using suboptimal parameters. 
B1
002-097493184F
TAGTAGAGGCGGGGTTTTGCCA
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
B2
002-097493184R
AAAAAGGGACAGTGTCCACTGGG
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
C1
015-081145141F
GCGTTAAGTGTCTGGAAGGCG
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
C2
015-081145141R
GGAACTCTCAGCCACAACAAACAC
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
D1
014-093175798F
CTCCCTACTACCGAAGAGGTC
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
D2
014-093175798R
GATCTGGAGGAGTCCAGGCA
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
E1
001-109847447F
GCAGGCTTGCCAAACTAAAAGCTC
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
E2
001-109847447R
TCAGAGAGCCAACAGAGCCC
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
F1
004-118702135F
GCAGGGACACTGGTGTTATGTCT
S2_S5_NN_Ranking-RVDs_5TC	Warning! Primer pair was generated using suboptimal parameters. 
F2
004-118702135R
GATAGTAACTTATTTAGAATTGAGGTTGTGTGATGGAG
S2_S5_NN_Ranking-RVDs_5TC	Warning! Primer pair was generated using suboptimal parameters. 
G1
003-060011649F
GTACTGCCAGAGTGTCAAGAAAGC
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
G2
003-060011649R
TAGGAAGAGGACAGTAGGCTAGG
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
H1
003-138953648F
CTTGGTGTCTGAGTGGCAAAACTG
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
H2
003-138953648R
TATGGGCCTGTTTTACACGGGCCT
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
A3
022-047592728F
AATGAGGCCTGGGAGCAGGA
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
A4
022-047592728R
CACCAAGAGCACCCCAAAGG
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
B3
012-062445219F
GAGAGGATACAAGGTCAGGTATGG
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
B4
012-062445219R
GGCCCATTTTAGAAAACATTTGCTGACTCC
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
C3
014-024242484F
CAAGTCTTCCTCAGCCATCAGACA
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
C4
014-024242484R
CCAGACACATCAACAGAGTGGAAG
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
D3
001-153175860F
CTCATAAGTGGAGGTCATTTCCCC
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
D4
001-153175860R
GCACACACCAGTTCTTACCACTGA
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
E3
00X-148727617F
GAGTCTTCCATAGCTGAGTGCAAC
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
E4
00X-148727617R
ACCCAACATCTGCCATCTGCAAG
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
F3
005-166596052F
CGTTGCCCAGCTTGATGCCT
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
F4
005-166596052R
GGAAGTTAGGCCCTAGGACC
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
G3
017-045018304F
TAACAGTGTGACTCTCTCCACCG
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
G4
017-045018304R
GTACAGAACTCCGCAGTGAAGTC
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
H3
005-137519020F
GCGAGGATCAAAATGGCAATCCC
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
H4
005-137519020R
GGCAGAGGACTAATGTTTCCTGG
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
A5
003-030699112F
CTGGCACAGAGTGAGCACTTAATG
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
A6
003-030699112R
GGTCGTCATGAGGAGAACACCTT
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
B5
011-012966658F
TGAGGGATAGAGCAGAGGATGG
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
B6
011-012966658R
GGGGGAATGATGTCACTGGAAC
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
C5
014-095952113F
GCCTGGATCCCACTGGACTA
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
C6
014-095952113R
ACAGCCCTTCTCCCTTCTGC
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
D5
017-009569404F
GGGAATGAAAAGGACAGCAGGAC
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
D6
017-009569404R
CGGCCCTGCGCGTTATTTCT
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
E5
006-088451104F
GATCTCCTTGAAGGCACATGCAG
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
E6
006-088451104R
GGCCTGGGAAACATAATGACACC
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
F5
008-027202946F
ATGTTGACATCTGGGCCACAGC
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
F6
008-027202946R
CCTCTCATCTCCTCCTTTGATCAG
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
G5
002-000581727F
CTCGGGCCATTGATTTGGCAAG
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
G6
002-000581727R
CCCCAGGGCACCTTTATGTC
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
H5
020-048858983F
TACTAGATGCTCAATACCTGGCGG
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
H6
020-048858983R
CTTGGGAAGCCACCTGGTCA
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
A7
019-017971876F
CTCAATTAATCCTCCCTGGAGACC
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
A8
019-017971876R
ATTCGCATGCGGTAGAGGGG
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
B7
014-040804929F
CCACCACGCCTGGCTAATTTTTC
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
B8
014-040804929R
GCCCACCCATCAGAGAGTTTC
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
C7
001-024242432F
TCCCAAGAGCAAGACATTGGCAG
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
C8
001-024242432R
GCAATGGAGGACCAGGCGTT
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
D7
003-041668393F
CTGGCACAGCTGTATTTCATGTCC
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
D8
003-041668393R
GTGATCTCCATGGACCTGTCCT
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
E7
003-137780481F
AGAGCCTGGCCATTTCTGCAC
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
E8
003-137780481R
TAGGTCTTTTGGGACATGGTTGCC
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
F7
003-058824763F
GCCAACTGATATATCTGGAGTGGG
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
F8
003-058824763R
TGCCAGGCCTTCCACAAGGT
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
G7
010-085871707F
AGTACACTTGGACCCGGCTTC
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
G8
010-085871707R
TGAATCCCAGGTAGCGTACCTGC
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
H7
001-045422945F
GCCCCCAAACACCACAAAGTC
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
H8
001-045422945R
ATTACAGGCACATGCCACCACG
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
A9
004-190659833F
CTGCCTCCAATCCCTCAGAAAATG
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
A10
004-190659833R
CTGGTATTGGGTCCAGACCAC
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
B9
001-045770634F
AATGGGGACGCTTCCGACTC
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
B10
001-045770634R
TTGTGGGGCCCCAAGATTTCC
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
C9
007-118742205F
GACAACCTGGTGAAGGCAACAGA
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
C10
007-118742205R
GTGCAGACATCCAGGATAAAACCC
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
D9
017-072308236F
AGGAGCAGACCGATGAGGAG
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
D10
017-072308236R
GGGATGCTTTGGAAGGAAGGG
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
E9
001-018569392F
TCATGTGACCCGCACCCTTG
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
E10
001-018569392R
GGGTCCAGAGCTTCCTAAAATCG
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
F9
002-104369295F
AGGAAGGGAAACTTCACCTCCTG
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
F10
002-104369295R
CCACCACCACAGTCAAGATGC
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
G9
020-039770399F
CCAGCTGGGATCCTCTGTTTC
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
G10
020-039770399R
CCTAGCACTGGGCAAATGGG
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
H9
002-235805535F
ACCTCCACCAGACCACGAATG
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
H10
002-235805535R
GACCCTTAGCAGGTCACACTC
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
A11
003-051035046F
CTTCTTAGAAATGGGACTCAGAAAGGACC
S2_S5_NN_Ranking-RVDs_5TC	Warning! Primer pair was generated using suboptimal parameters. 
A12
003-051035046R
CAATTGAGAGAAGCAGCAAAGTCTTCTACAG
S2_S5_NN_Ranking-RVDs_5TC	Warning! Primer pair was generated using suboptimal parameters. 
B11
017-068378451F
TGGGCTTCCTCCATGTTGGTC
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
B12
017-068378451R
ATGACAGGACCTGGCTCACC
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
C11
010-034494294F
GCTAGATAGGCCGGGTGAAATG
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
C12
010-034494294R
AGCTCCCAGGCTTTCACCAC
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
D11
019-055659570F
ACCGAGGACATTTCCTAAGAAGGG
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
D12
019-055659570R
GTGGGTATTGGGATTTGGGGC
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
E11
010-075583632F
GGTATAATGCCCTTCCTCAGCATG
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
E12
010-075583632R
CAGGGAAGAGCCATAGTCTGTC
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
F11
001-067142359F
GGTACCTGAAACAGAGCCAGTACA
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
F12
001-067142359R
GAACCCCATATCTCAGGCTGATC
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
G11
017-009569404F
GGGAATGAAAAGGACAGCAGGAC
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
G12
017-009569404R
CGGCCCTGCGCGTTATTTCT
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
H11
010-085944463F
TGCAAGCTTGAGCCAGAGCC
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
H12
010-085944463R
GAGGTTGGCTCATGATAGCTCTG
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
Primer Plate #3
A1
006-165472416F
CCTCTGCCTCGGGTTATATGAAG
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
A2
006-165472416R
CGAAGGAATGCATTGCATGTTGGC
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
B1
015-086366341F
AGATATGGGGGTCTTGCCATGTTG
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
B2
015-086366341R
TATGGGCGTTTCTCTAGGGAGAAG
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
C1
010-079846352F
GCTGCTCGAACCACACAACTAG
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
C2
010-079846352R
CTTAAGAACTGTCACCCTGGAGAG
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
D1
001-211904919F
GCAGTGTCATGGGATCAAGGATGT
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
D2
001-211904919R
TGCCTAGAACAATGGCTGGCATG
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
E1
006-097366253F
TACCTTCAGGCTGATATGGCTTGG
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
E2
006-097366253R
CTGAATGTGAAGATGTCTCACAGTAGCC
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
F1
014-105602839F
TCCATGCCATGCGTGGCTGT
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
F2
014-105602839R
ACGGTAAGGCCTGGGGAAAC
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
G1
008-041570215F
GGGTGCAGAGAGATTTGCATGTG
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
G2
008-041570215R
TGTCTCCTCCTGTCCCTGCT
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
H1
006-048618042F
CCTCCTGTCTTTATTAGTCTAGCAAGAAGTTTGT
S2_S5_NN_Ranking-RVDs_5TC	Warning! Primer pair was generated using suboptimal parameters. 
H2
006-048618042R
GTGGTGTCCTGGATAAAATCCTGAAACAGA
S2_S5_NN_Ranking-RVDs_5TC	Warning! Primer pair was generated using suboptimal parameters. 
A3
018-072086920F
GCTGTCACCAGGAAAAACATAAGAGCC
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
A4
018-072086920R
AAGCTGCTGAAATGGGCAGAGG
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
B3
018-014372203F
CCTTGGGAACCTTGGACAATAAGC
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
B4
018-014372203R
GTCCCCTCTCCTTGCACTTTTC
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
C3
013-019260513F
GTCCCCTCTCCTTGCACTTTTC
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
C4
013-019260513R
CTTGGGAGCCTTGGACAATAAGC
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
D3
016-089119131F
CTCGCGGCTGAGTATAGGCA
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
D4
016-089119131R
GTTTCCTCTTCTTCTTCCTGGTGG
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
E3
003-010453381F
TGTTGCAGACACTGGAACAGAGC
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
E4
003-010453381R
CGTGAGAGTGGACTTGCAAGTCTG
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
F3
013-073489472F
GCGCTCTGCTTCTAAGACACATTG
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
F4
013-073489472R
CACATGGAAGAGATGATACATGCCATTACC
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
G3
001-227379954F
GAGATCACTCTCACCACCCAAAAC
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
G4
001-227379954R
GGAAACAGCAGAACGTTGTTGGTG
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
H3
004-085199195F
CAGTTCTACTTCAGGGAAGGGG
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
H4
004-085199195R
GGATACAAGTGGAAAAGCACAGCC
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
A5
017-020954067F
CGGGAAAAAGTACCAGATGGAGAC
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
A6
017-020954067R
GACAGAGTGAGACCCTGTCTCAA
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
B5
003-082432463F
GACAGGGAAGAAACTCTGCAAACC
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
B6
003-082432463R
GCACGGATATGCAATAAACTTCTGTTTCCC
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
C5
00X-056339296F
GCAAGAATATTGGCAATACTGTCCCATGTC
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
C6
00X-056339296R
GCAGAGGCCTAGTCTGAAGC
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
D5
011-109690484F
GCAGAGGCCTAGTCTGAAGC
S2_S5_NN_Ranking-RVDs_5TC	Warning! Primer pair was generated using suboptimal parameters. 
D6
011-109690484R
AAGCAAGAATATTGGCAATACTGTCCCATGTC
S2_S5_NN_Ranking-RVDs_5TC	Warning! Primer pair was generated using suboptimal parameters. 
E5
011-125968061F
GACACTAGAAATCTGGGTACCCTG
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
E6
011-125968061R
ACAGCTGTTTGCTCCGTGGG
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
F5
018-030266951F
GAATTAACAAGGGAGGTCATGTAAACTGGC
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
F6
018-030266951R
CGTGCTGTGTTAGGTATTCCCTC
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
G5
006-063449762F
TCTGAGGAGACCTTCAGAGGG
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
G6
006-063449762R
ACCACCTCTTGAAGCAGCTCTC
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
H5
007-002962408F
ACAGCGTTTGTCTCCTGGCC
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
H6
007-002962408R
GCCCCTAAGCCTTGCATTCAG
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
A7
011-099262817F
CTTGGACTACCCTAAGGCTCAG
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
A8
011-099262817R
TCAGATGGGCTGGATGAAAAGTTAGCCT
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
B7
016-088575852F
CCATTAGGCACACAGGGAGG
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
B8
016-088575852R
CTGCCTCCTGAACCCACTCT
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
C7
007-158980941F
GCCCAACTACATGCTGGGTC
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
C8
007-158980941R
GTGGCCTATCTTGATGAATGTCCC
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
D7
019-047560548F
CTGCGTTGCCTGGGATAGTC
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
D8
019-047560548R
GCTATGTGGGGTCAGGCAGT
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
E7
002-011017617F
GAGCTTGGTGAATAGAAGGTACCC
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
E8
002-011017617R
CTCCCCCAAGCACTCCATTTAAC
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
F7
004-110007835F
GACTGCTGGGAATTGCTCTTTGAC
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
F8
004-110007835R
TCCCTCAACTTCCACTGGGC
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
G7
009-066282880F
ACTACAGGTGCACACCACCATG
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
G8
009-066282880R
GCCTTCCCCAAGTTTGCATTTTCG
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
H7
019-057866958F
GCCTCCGTGTTTCAATTGCTCTC
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
H8
019-057866958R
GGGCTGGTTGTCAGTATAGGG
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
A9
010-134734250F
ACTCAACGGCATCCTCCCCT
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
A10
010-134734250R
ATCCACCGCCCTCAGATGCT
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
B9
020-045428486F
GTCTCACTGAGACCTAGGCG
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
B10
020-045428486R
TGGGCCTGGGGAACCAACAA
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
C9
018-000763240F
CCAGGGAGCATTAGGAATCCAG
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
C10
018-000763240R
TTTGTAGAGACGGGGTTCTGCC
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
D9
015-090111045F
GCCTTCCTCTCCTCATACCC
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
D10
015-090111045R
TACAGGTGTGAGCCACCACG
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
E9
006-138657515F
GTTCCCGTGCATCAGTCAGC
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
E10
006-138657515R
GCTGATCAGTGGTTGGAAAGGC
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
F9
001-231005091F
GCGACATCCCAACAGGTAGG
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
F10
001-231005091R
CGGAAGATGAGGCCATCTCAC
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
G9
018-055555293F
TCTGGGAAGAAGCTGCCAGG
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
G10
018-055555293R
GGCTATGCTACCTGCTGTAAAACC
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
H9
019-002162216F
TAGTAGAGACGGGGTTTCACCATG
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
H10
019-002162216R
TTTCCGCCATGTTGGCCAGG
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
A11
00X-106025404F
CTTTCCCCAACTGTGTGATACAGC
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
A12
00X-106025404R
CTGGTACTGGAACTCTGCTCTTCA
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
B11
005-137808181F
CGTTCAGCCTCGTTCTCACC
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
B12
005-137808181R
CAATGGCTTCCCCTCTCCAAC
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
C11
004-190407488F
CCTGGGGGAACTTTTTCAGTCCAT
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
C12
004-190407488R
GGTTTCACATGCCCTGAATACTGC
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
D11
008-121927538F
GCAGTGATGAAATCACACTGGCC
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
D12
008-121927538R
GCCAAGAAACACTAGCCACAGTC
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
E11
003-157302016F
ATCCCAGGTAGCGTACCTGC
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
E12
003-157302016R
CTTAGTACACTTGGACCTGGCTTC
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
F11
019-009837811F
AATCCCAGGTAGCGTACGTGC
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
F12
019-009837811R
TCAGTACACATGGACCTGGCTTC
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
G11
005-143474515F
CCACTCTCCCTCAAATCAACTGC
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
G12
005-143474515R
CTGCATTTCTGAGACAGACATGGC
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
H11
003-102226111F
CCAGCATCATTGAAAGCTGGCAGA
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
H12
003-102226111R
CTGTATCCCCATCATGTGCTGAAG
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
Primer Plate #4
A1
002-170227380F
GGCCAGAAGACCAAGATGATTCC
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
A2
002-170227380R
GCCAAAAGCCAAATGTAGACTTTGTGCTG
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
B1
013-099191073F
AGGCCCTGGTGTCCCTTCTT
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
B2
013-099191073R
TGATGGAAACAGCGGGTGCTC
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
C1
002-241334168F
CATGCACCCCTGGCTTGTGT
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
C2
002-241334168R
AGCTTTGCACCTGGAACCTGTGA
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
D1
004-068122758F
CTTTTGCTTGTTGGTTCGGTGGC
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
D2
004-068122758R
GTCCTGATGGAGTCTAAATGGCTG
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
E1
008-011765812F
ATCCCCAGTAATGTACCTGCTGTC
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
E2
008-011765812R
CTCAGTACACTTGCACCCGG
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
F1
008-142885698F
GTGAACTACCTCTCTGGCACTG
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
F2
008-142885698R
CTCACCCAAAACTGGAAGCAACC
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
G1
022-024555541F
CACAGCCATTCTGTCTACTCCC
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
G2
022-024555541R
TGGCAGGTGGCCAGTGTCTT
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
H1
004-024170953F
CTGGATGGGATCCCAGAACAGA
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
H2
004-024170953R
GAGCCAACATGCTTGGCTGAG
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
A3
011-117929965F
GCTCCGATGTGAGAAGTCTCTAG
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
A4
011-117929965R
CCTTCCACACATTCTGCACTCTG
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
B3
009-087590855F
CAAGGGGAAGGAGTGGTTATCTAG
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
B4
009-087590855R
GTCTCAGACTTCTAGCCTCCAG
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
C3
012-109714286F
CCCCATGTCTCCTGTATGCTTTC
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
C4
012-109714286R
ACTTTGCAGGAGCTAGGGGAC
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
D3
022-036626609F
CTGACTCCAGGCTCTGACAC
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
D4
022-036626609R
GCTGGGGCCTTTTTTCTCATCTG
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
E3
016-030831779F
CACACACACACACACATGGGC
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
E4
016-030831779R
ACTGTCTGGGGAAGGGATGG
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
F3
019-044278490F
GTAGGAAGCGTTGAGCAGGAC
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
F4
019-044278490R
TCCTGACCCCTCCCTTCTTG
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
G3
016-084229758F
TCTGAGCAGGGCCATCCACA
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
G4
016-084229758R
TGCATGAGCCCTCCATCACC
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
H3
021-043483081F
TGGAGGCTGCGAGGGAGAAA
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
H4
021-043483081R
GCACCCCTGCAAAAGATATGTCC
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
A5
006-153846994F
GCAAGCTGTCTCATGCTCACTG
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
A6
006-153846994R
CAGGAAGGAACTTGTCAGATTCCC
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
B5
009-138280949F
CGCAGAGGCTCTTTGTGAGCT
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
B6
009-138280949R
CTCCAGCTCCTGCATGTCATTC
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
C5
010-101944614F
CCAATTTCTCCACAAACTGGCCTC
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
C6
010-101944614R
GTTTGCCGCTGTAGATGCTGTATG
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
D5
004-170092193F
GACCTAAAGGTTTCTCCACACATAGTGAAG
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
D6
004-170092193R
GACTGTTCTGAGATCTTGGGTGTG
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
E5
013-100940227F
GTGGTAGACCTTCAGAATTTTATGAGGGAG
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
E6
013-100940227R
TCACCACTGCACTCCAGCCT
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
F5
007-044570049F
GGAGATGGATGGTGGTGATAGTTG
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
F6
007-044570049R
TTAGGCAGTACTGGCCTGGG
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
G5
00Y-015000077F
CAGGTGTGTGTCAACAAGCCC
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
G6
00Y-015000077R
CCAGTCTAGGCAACAGAGTGAG
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
H5
010-117176374F
CAATATCTCAGTATTATCACTTTGAAAACACTTGAGGG
S2_S5_NN_Ranking-RVDs_5TC	Warning! Primer pair was generated using suboptimal parameters. 
H6
010-117176374R
CTTGGGGATCAGGGCCATTTC
S2_S5_NN_Ranking-RVDs_5TC	Warning! Primer pair was generated using suboptimal parameters. 
A7
00X-152817806F
TCACCAGCCTCCCATGCAGT
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
A8
00X-152817806R
GGCAGCTCCACCACAGACTT
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
B7
001-231353429F
AAGGCTGTGGAGCCTCCACCAT
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
B8
001-231353429R
AATCCCAGCCCCCAAGCTCA
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
C7
00Y-002494311F
ACAGGAGGGAATCCTGTGTTGG
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
C8
00Y-002494311R
GACCTGGAAGCAGATGGAGAAAAC
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
D7
00X-002544311F
ACAGGAGGGAATCCTGTGTTGG
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
D8
00X-002544311R
GACCTGGAAGCAGATGGAGAAAAC
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
E7
010-052784032F
ACTGAGGCCCAAATCATAGCCAATGATGGA
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
E8
010-052784032R
AGGCCTCCACAGCCATGCTT
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
F7
010-107873570F
GACACCTTCTGCTAAGCACACATC
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
F8
010-107873570R
GAGCTCATTCAAAGCCCCAGTAG
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
G7
002-191816679F
CCAACAGTTTGGGAGGCCAAG
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
G8
002-191816679R
CTGCCCAGAGTCCAGAACAGT
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
H7
008-102790216F
GGGAGAAGGAACAGTCAAGGC
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
H8
008-102790216R
TGAAGGGCTGGGTTGACTGG
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
A9
018-073844625F
CCGCAATGGGATAAGAGGCAAC
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
A10
018-073844625R
GCGCTCCTGTTAAAAATCCGGAGT
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
B9
002-138683178F
CTGTCCCCTCTCCTTGTACTTTTC
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
B10
002-138683178R
GGGAGCCTTGGACAATAAGCTG
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
C9
011-031965225F
CCAGCCTGAGACAACCATCTTTAC
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
C10
011-031965225R
GAAGGGCCATAACTTGGGCATTAG
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
D9
007-154052611F
GAATATCTTTTTGAGTATGGTGTGAGGTAGGG
S2_S5_NN_Ranking-RVDs_5TC	Warning! Primer pair was generated using suboptimal parameters. 
D10
007-154052611R
GAGTATGTGTGTGTAATTTGACAAGCCGATTC
S2_S5_NN_Ranking-RVDs_5TC	Warning! Primer pair was generated using suboptimal parameters. 
E9
001-213140398F
ATTCTAAGAGTGGCGTGTGGAGG
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
E10
001-213140398R
GTTGTGGGTGATTCCAATGGCATG
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
F9
001-230976433F
GCGCCAAGGTCTTCTTGAGC
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
F10
001-230976433R
GGAGAGTAGGAGACAGCACATG
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
G9
014-038458927F
CGTTGCAATTTCCCATCCGCATGT
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
G10
014-038458927R
CCTGGGGTTTTCTTTGTGGAAGGA
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
H9
003-004440767F
TATGAGAACGAGAGGGGGCG
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
H10
003-004440767R
CAAGCCCTTCCCCACTGTAG
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
A11
00X-010361133F
CCGGATCTTCAACAAGCCCC
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
A12
00X-010361133R
CATCCTACATGAGAAAGAGCCAGC
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
B11
009-009921803F
CAGCTGATATTATGTGTCCCACACTGTTC
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
B12
009-009921803R
GAGTTGCTCACAGCTTCTCTGAGT
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
C11
010-131523914F
CTCGGAGGTCTGTCCATGCT
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
C12
010-131523914R
GAGCTAGAAGGGGCTGGTTG
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
D11
010-045378787F
GGTGAGGCTCCATCCCACAA
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
D12
010-045378787R
ACCCAGAGAGGGTGTGAGAG
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
E11
022-024444126F
TGCTCTCTCCTCCCAAGCAC
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
E12
022-024444126R
GGTCTCACCAGTGAACACACAG
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
F11
004-043460367F
GCTAAGAGCCTCTTGGGCTTTTG
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
F12
004-043460367R
AGCAGAGGCAGAAAGGACCAG
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
G11
008-142398434F
GCCTGAGATAGGGACATTGGTG
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
G12
008-142398434R
CCCATTCTGAGCGAGCTTGGA
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
H11
009-019336805F
GTCCATTGATAATCACTCTAGCACAGGTAC
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
H12
009-019336805R
GTGTGACTTCCTTCCTTCCCTC
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
Primer Plate #5
A1
005-001179126F
AAGCTGCACGCAGCCCTCAT
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
A2
005-001179126R
GTCTTGAGGCCCCAGGGAAA
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
B1
001-230749531F
CAAAGATGCAAAGGCTGATTTAGAGCACC
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
B2
001-230749531R
CCCCAAATTTAGCAATACTGTCTGCTGGA
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
C1
001-186925307F
GGCCTTGGTGAGTGATTCAGC
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
C2
001-186925307R
CCCTAGTCATTTGAAGCCCTTCAC
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
D1
019-035978213F
CAGATACAGGAGCTGTTGGATGG
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
D2
019-035978213R
CTGAAGGGAGGTCTTAGCTACTAC
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
E1
011-056386094F
GGGAGGTACTATGCCTGGATG
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
E2
011-056386094R
GCTCGGGAGGATTAGTAAAAGAGG
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
F1
002-028093265F
GCTGCCAGTGGTGACTGTTTTTC
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
F2
002-028093265R
GTGAGTCTACACACCATGTCATCC
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
G1
004-188278629F
CCTTTCCCTTACCCTGTTTGTTCC
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
G2
004-188278629R
GTGTCCTAGGAACACTGCTCTG
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
H1
002-232990297F
GGTAATATAGGCGAGCATGCTGTG
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
H2
002-232990297R
GAAAACACAAGTCCTCACAGGGG
S2_S5_NN_Ranking-RVDs_5TC	Primer pair generated using optimal parameters.