PROGNOS Summary for S2_S5_NN_Ranking-RVDs_5T

The hg19 genome was searched for the TALEN pair targeting:
5'- TCACCTTGCCCCACAGGGCAGT NN..NN ACAGGAGTCAGGTGCA -3'

Using these RVDs:
Left:  01HD02NI03HD04HD05NG06NG07NN08HD09HD10HD11HD12NI13HD14NI15NN16NN17NN18HD19NI20NN21NG
Right: 01NN02HD03NI04HD05HD06NG07NN08NI09HD10NG11HD12HD13NG14NN15NG

Up to 5 mismatches were allowed in each nuclease half-site.

Allowed spacing distances were: 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30

Homodimers and heterodimers of the nucleases were included in the output

3481 total sites were located
Mismatch Types
Genomic Regions
  0 and 2: 1
  2 and 2: 1
  3 and 3: 3
  2 and 5: 8
  3 and 4: 18
  3 and 5: 112
  4 and 4: 63
  4 and 5: 813
  5 and 5: 2462
  Exons: 143
  Promoters: 28
  Introns: 1383
  Intergenic: 1927


The top 200 sites are ranked below according to a weighting of the RVD base preferences. All sites listed contain flanking 5' thymidines.
For the full output, click here.

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Primer pairs were designed using the 400 bp of sequence on either side of the cleavage site to melt close to 60 °C

For easy visualization of cleavage on gels:
The smallest fragment will be longer than 130 bp, which is also the minimum separation distance between uncleaved and cleaved products.
The two cleavage products will be separated by a minimum size of 0 bp
To optimize assay conditions, the amplicons have a minimum length of 260 bp, a maximum length of 450 bp, and an optimal length of 270 bp.
Rank: 1	Homology Score: 52	RVD Match Score: 2.328	Left Half-Site RVD Score: 3.661	Right Half-Site RVD Score: 4.49
	Genomic Region: Exon	Closest Gene: HBB	Caution! This site scores very closely to the intended nuclease target. The potential for cleavage at this site is of great concern.
Match Type: L16R
	           Left Nuclease
	         TCACCTTGCCCCACAGGGCAGT
	5'-CACGT TCACCTTGCCCCACAGGGCAGT        AACGGCAGACTTCTCC              TCAGGAGTCAGATGCA CCATG-3'
	3'-GTGCA AGTGGAACGGGGTGTCCCGTCA        TTGCCGTCTGAAGAGG              AGTCCTCAGTCTACGT GGTAC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 011-005248069
Forward Primer: 5'-GTGCCTATCAGAAACCCAAGAGTC-3' Tm: 59.038
Reverse Primer: 5'-CAATCTACTCCCAGGAGCAGG-3' Tm: 59.378
Expected PCR Product Length: 308
Expected CelI Cleavage Product Sizes: 154, 154
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr11:5248194-5248247

Rank: 2	Homology Score: 32	RVD Match Score: 2.561	Left Half-Site RVD Score: 5.563	Right Half-Site RVD Score: 4.49
	Genomic Region: Exon	Closest Gene: HBD
Match Type: L16R
	           Left Nuclease
	         TCACCTTGCCCCACAGGGCAGT
	5'-CACGT TCACTTTGCCCCACAGGGCATT        GACAGCAGTCTTCTCC              TCAGGAGTCAGATGCA CCATG-3'
	3'-GTGCA AGTGAAACGGGGTGTCCCGTAA        CTGTCGTCAGAAGAGG              AGTCCTCAGTCTACGT GGTAC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 011-005255477
Forward Primer: 5'-GTCAGTGCCTATCAGAAACCCAAG-3' Tm: 59.038
Reverse Primer: 5'-TCACTGGAGCAGGGAGGACA-3' Tm: 59.508
Expected PCR Product Length: 296
Expected CelI Cleavage Product Sizes: 158, 138
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr11:5255606-5255659

Rank: 3	Homology Score: 10	RVD Match Score: 2.7	Left Half-Site RVD Score: 5.479	Right Half-Site RVD Score: 3.87
	Genomic Region: Intergenic	Closest Gene: FAM47A
Match Type: R10R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-AGTCT TCACCCTTACTCCTAT                 GCTTATTCTA                 ACAGGAGTTAGATGTA CTTCT-3'
	3'-TCAGA AGTGGGAATGAGGATA                 CGAATAAGAT                 TGTCCTCAATCTACAT GAAGA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 00X-034076224
Forward Primer: 5'-AGTATTGCCCTTTCTGCTAGGGAC-3' Tm: 59.038
Reverse Primer: 5'-CACTGCACACTTCTGTGGCTC-3' Tm: 59.378
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 134, 136
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chrX:34076335-34076376

Rank: 4	Homology Score: 18	RVD Match Score: 2.728	Left Half-Site RVD Score: 4.957	Right Half-Site RVD Score: 4.522
	Genomic Region: Intron	Closest Gene: PHF21B
Match Type: R12R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CATTC TCCACCTGTCTCCTGC                CTCTCAACTGGG                TCTGGAGTCAGGTGGA AAAAT-3'
	3'-GTAAG AGGTGGACAGAGGACG                GAGAGTTGACCC                AGACCTCAGTCCACCT TTTTA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 022-045287459
Forward Primer: 5'-CAAAGGCTCTGCCTGCAGGA-3' Tm: 59.508
Reverse Primer: 5'-CAGGACTAAGGCCGCTCTTC-3' Tm: 59.508
Expected PCR Product Length: 269
Expected CelI Cleavage Product Sizes: 136, 133
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr22:45287571-45287614

Rank: 5	Homology Score: 17	RVD Match Score: 2.745	Left Half-Site RVD Score: 4.174	Right Half-Site RVD Score: 5.455
	Genomic Region: Intergenic	Closest Gene: GPR6
Match Type: R30R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CCATC TCCACCTGGCTCCTGT       GTGGTCCATGTTGCAAGTTTTCAGGAAGGA       GCAGGAGTTAAGGGTA GAGTT-3'
	3'-GGTAG AGGTGGACCGAGGACA       CACCAGGTACAACGTTCAAAAGTCCTTCCT       CGTCCTCAATTCCCAT CTCAA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 006-110284189
Forward Primer: 5'-GCAATGTTGCTCCAAGAGGCC-3' Tm: 59.378
Reverse Primer: 5'-GAACTTATTCCTCCCACCTAGCTG-3' Tm: 59.038
Expected PCR Product Length: 297
Expected CelI Cleavage Product Sizes: 131, 166
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr6:110284287-110284348

Rank: 6	Homology Score: 18	RVD Match Score: 2.782	Left Half-Site RVD Score: 4.867	Right Half-Site RVD Score: 4.984
	Genomic Region: Intron	Closest Gene: FAM3D
Match Type: R17R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CCCTG TGCCCCTGACTCCTTA             CAGCTGTTCTAGAATGT              AAATGAGGCAGGTGCA TTTCG-3'
	3'-GGGAC ACGGGGACTGAGGAAT             GTCGACAAGATCTTACA              TTTACTCCGTCCACGT AAAGC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 003-058640715
Forward Primer: 5'-CATATGAGGAAACTGAGAGGCGG-3' Tm: 59.148
Reverse Primer: 5'-CCTCGAGGAGGGCTATTCTAGA-3' Tm: 59.258
Expected PCR Product Length: 283
Expected CelI Cleavage Product Sizes: 130, 153
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr3:58640819-58640867

Rank: 7	Homology Score: 13	RVD Match Score: 2.8	Left Half-Site RVD Score: 4.387	Right Half-Site RVD Score: 5.628
	Genomic Region: Intergenic	Closest Gene: HYDIN2
Match Type: R26R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CCCTC TGCACACAACTCCTAT         GCACTCTGCACGCACCACCCTCCTGC         ACAGGTGTCCCGTGCA GGCTG-3'
	3'-GGGAG ACGTGTGTTGAGGATA         CGTGAGACGTGCGTGGTGGGAGGACG         TGTCCACAGGGCACGT CCGAC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: dom-000216374
Forward Primer: 5'-TCTCCTTTCTGTACCTCAGAGACC-3' Tm: 59.038
Reverse Primer: 5'-CCCACCCTGGTACAGCAAATG-3' Tm: 59.378
Expected PCR Product Length: 318
Expected CelI Cleavage Product Sizes: 131, 187
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr1_gl000192_random:216474-216531

Rank: 8	Homology Score: 18	RVD Match Score: 2.811	Left Half-Site RVD Score: 4.571	Right Half-Site RVD Score: 5.505
	Genomic Region: Intron	Closest Gene: PDE4D
Match Type: R29R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CACAC TGCACCTAACATCTGT       CCTTATAATAAACTAGGAGCATCTCTGTT        ACAGGAATCATGTTCA GTAGT-3'
	3'-GTGTG ACGTGGATTGTAGACA       GGAATATTATTTGATCCTCGTAGAGACAA        TGTCCTTAGTACAAGT CATCA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 005-059619945
Forward Primer: 5'-CCAAGACTTAGCTTAGCACCCAAC-3' Tm: 59.038
Reverse Primer: 5'-CATGCCTGGAGTTAGCCTCTCT-3' Tm: 59.258
Expected PCR Product Length: 384
Expected CelI Cleavage Product Sizes: 219, 165
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr5:59620132-59620192

Rank: 9	Homology Score: 10	RVD Match Score: 2.816	Left Half-Site RVD Score: 5.523	Right Half-Site RVD Score: 4.592
	Genomic Region: Intron	Closest Gene: C14orf166B
Match Type: R24R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TAACT TGCCCCCAACTCATCT          TTTCTTAGATTAGCTCTGGCAATC          ACAGGAGGCAGATGTA ACAGT-3'
	3'-ATTGA ACGGGGGTTGAGTAGA          AAAGAATCTAATCGAGACCGTTAG          TGTCCTCCGTCTACAT TGTCA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 014-077327823
Forward Primer: 5'-CCAACAGCTGGTAAGGATGTCAAG-3' Tm: 59.038
Reverse Primer: 5'-CTGTGGAAACACCAAAGCAGGG-3' Tm: 59.258
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 133, 137
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr14:77327926-77327981

Rank: 10	Homology Score: 10	RVD Match Score: 2.817	Left Half-Site RVD Score: 3.821	Right Half-Site RVD Score: 6.445
	Genomic Region: Intron	Closest Gene: COL23A1
Match Type: R29R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-ATCCC TGCACCTCACACCTAT       CCTGAAGCAAACTAATCAGTAGTTAAATA        AGAGGAGGAGGGTACA TTACG-3'
	3'-TAGGG ACGTGGAGTGTGGATA       GGACTTCGTTTGATTAGTCATCAATTTAT        TCTCCTCCTCCCATGT AATGC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 005-177943719
Forward Primer: 5'-AAGCTTTCTGCCTCCTCAGGCC-3' Tm: 61.288
Reverse Primer: 5'-CACCTCAGTGACAGGCGCTT-3' Tm: 59.508
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 137, 133
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr5:177943824-177943884

Rank: 11	Homology Score: 10	RVD Match Score: 2.832	Left Half-Site RVD Score: 4.963	Right Half-Site RVD Score: 5.244
	Genomic Region: Intron	Closest Gene: SLC2A13
Match Type: R19R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TAAGA TTCAACTGACTCCTGA            GTTAGATGCAAAAAATTTT             ATAGTAGTGAGGTCTA CTGTT-3'
	3'-ATTCT AAGTTGACTGAGGACT            CAATCTACGTTTTTTAAAA             TATCATCACTCCAGAT GACAA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 012-040234817
Forward Primer: 5'-CCTTACAATTCACGGCTCTCAGAC-3' Tm: 59.038
Reverse Primer: 5'-CCACTGGAGTTGACAGATTTGGC-3' Tm: 59.148
Expected PCR Product Length: 436
Expected CelI Cleavage Product Sizes: 130, 306
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr12:40234920-40234970

Rank: 12	Homology Score: 10	RVD Match Score: 2.833	Left Half-Site RVD Score: 3.821	Right Half-Site RVD Score: 6.578
	Genomic Region: Intergenic	Closest Gene: MIR4686
Match Type: R25R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TCGGC TCCACCTGACTCCTAC         ACTGGCTCTGCAACCATTCCCAGCC          ACAGAGGTTGGCTGCA AGTAC-3'
	3'-AGCCG AGGTGGACTGAGGATG         TGACCGAGACGTTGGTAAGGGTCGG          TGTCTCCAACCGACGT TCATG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 011-002239013
Forward Primer: 5'-AGCTGTCCAGCCTGCAGCAT-3' Tm: 59.508
Reverse Primer: 5'-TCATATTCCTGGGTGGTCACTCC-3' Tm: 59.148
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 138, 132
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr11:2239121-2239177

Rank: 13	Homology Score: 5	RVD Match Score: 2.833	Left Half-Site RVD Score: 4.837	Right Half-Site RVD Score: 5.385
	Genomic Region: Intergenic	Closest Gene: TBX22
Match Type: R28R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TCATC TGCACATAACTACTCT        CCTTTTGAGAAACAGTTCTTGGCCTGTT        ACTGGGGTTTGGTGGA AACTG-3'
	3'-AGTAG ACGTGTATTGATGAGA        GGAAAACTCTTTGTCAAGAACCGGACAA        TGACCCCAAACCACCT TTGAC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 00X-079108050
Forward Primer: 5'-GGTGTCAGTAGCAGATAGGGATG-3' Tm: 59.148
Reverse Primer: 5'-CTGTACCCAACCCACTTTATGGC-3' Tm: 59.148
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 136, 134
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chrX:79108154-79108213

Rank: 14	Homology Score: 5	RVD Match Score: 2.835	Left Half-Site RVD Score: 5.169	Right Half-Site RVD Score: 5.058
	Genomic Region: Intron	Closest Gene: ROCK2
Match Type: R10R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-ACTAC TGCACCTAACTCACAC                 AGAAAAGAGC                 AGAGGAGGTAGGAGCA ATTTA-3'
	3'-TGATG ACGTGGATTGAGTGTG                 TCTTTTCTCG                 TCTCCTCCATCCTCGT TAAAT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Warning! Primer pair was generated using suboptimal parameters. 
Primer Index: 002-011376315
Forward Primer: 5'-CCCCTCATGGAGAACACAATACAAAAGAAAC-3' Tm: 59.958
Reverse Primer: 5'-GAGAGAAGAGTTACGCGTCTTCC-3' Tm: 59.148
Expected PCR Product Length: 307
Expected CelI Cleavage Product Sizes: 169, 138
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr2:11376461-11376502

Rank: 15	Homology Score: 13	RVD Match Score: 2.838	Left Half-Site RVD Score: 4.387	Right Half-Site RVD Score: 5.923
	Genomic Region: Intron	Closest Gene: HYDIN
Match Type: R26R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CCCTC TGCACACAACTCCTAT         GCACTCTGCACGCACCACCCTCCTGC         ACAGGCGTCCCGTGCA GGCTG-3'
	3'-GGGAG ACGTGTGTTGAGGATA         CGTGAGACGTGCGTGGTGGGAGGACG         TGTCCGCAGGGCACGT CCGAC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 016-071012605
Forward Primer: 5'-CTCCTAGCTTCCTGATTCTTTGCC-3' Tm: 59.038
Reverse Primer: 5'-CCCACCCTGGTACAGCAAATG-3' Tm: 59.378
Expected PCR Product Length: 355
Expected CelI Cleavage Product Sizes: 168, 187
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr16:71012742-71012799

Rank: 16	Homology Score: 5	RVD Match Score: 2.847	Left Half-Site RVD Score: 4.565	Right Half-Site RVD Score: 5.782
	Genomic Region: Intron	Closest Gene: EXOC3L2
Match Type: R16R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-ACCTT TGCACCGAACCCCTAT              AGGGCGGGCACGAGGA              CCAGGAGATGGGTGTA TGGGG-3'
	3'-TGGAA ACGTGGCTTGGGGATA              TCCCGCCCGTGCTCCT              GGTCCTCTACCCACAT ACCCC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 019-045727578
Forward Primer: 5'-GAGAAGGGGAGTGTTCCACTG-3' Tm: 59.378
Reverse Primer: 5'-GGATAACAGAGGGCCATCAGC-3' Tm: 59.378
Expected PCR Product Length: 278
Expected CelI Cleavage Product Sizes: 148, 130
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr19:45727700-45727747

Rank: 17	Homology Score: 2	RVD Match Score: 2.851	Left Half-Site RVD Score: 4.351	Right Half-Site RVD Score: 6.062
	Genomic Region: Intron	Closest Gene: CEP55
Match Type: R21R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-ATATA TACACCCAACTCCCAT           ATCTGGGTAATTTGGATAATT            ACAGTAGTTATCTGGA TAAAT-3'
	3'-TATAT ATGTGGGTTGAGGGTA           TAGACCCATTAAACCTATTAA            TGTCATCAATAGACCT ATTTA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 010-095287016
Forward Primer: 5'-GAGCTGCTAAAATTGCTTGCTGCC-3' Tm: 59.038
Reverse Primer: 5'-GCACCCAGCCTGTTCAGCAA-3' Tm: 59.508
Expected PCR Product Length: 335
Expected CelI Cleavage Product Sizes: 179, 156
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr10:95287167-95287219

Rank: 18	Homology Score: 13	RVD Match Score: 2.856	Left Half-Site RVD Score: 5.066	Right Half-Site RVD Score: 5.318
	Genomic Region: Intron	Closest Gene: GRIA2
Match Type: R19R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-ATCCA TACACCTGTCTGCTGT            AACCTTTTCCAGATTGGCT             ACTGGAGTGAAGTGGA CAAAA-3'
	3'-TAGGT ATGTGGACAGACGACA            TTGGAAAAGGTCTAACCGA             TGACCTCACTTCACCT GTTTT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
No Primers
Primer Index: No Primers
Forward Primer: 5'-No Primers-3' Tm: No Primers
Reverse Primer: 5'-No Primers-3' Tm: No Primers
Expected PCR Product Length: No Primers
Expected CelI Cleavage Product Sizes: No Primers, No Primers
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr4:158255134-158255184

Rank: 19	Homology Score: 8	RVD Match Score: 2.857	Left Half-Site RVD Score: 4.408	Right Half-Site RVD Score: 6.04
	Genomic Region: Intron	Closest Gene: HOMER1
Match Type: R15R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-AAGGA TACCCCTAAATCCTGT              CATTCTCCCACTTAA               CCTGGATTCAGGTGAA GACCT-3'
	3'-TTCCT ATGGGGATTTAGGACA              GTAAGAGGGTGAATT               GGACCTAAGTCCACTT CTGGA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 005-078702362
Forward Primer: 5'-GAAAGGATCCTGGATGAGGGG-3' Tm: 59.378
Reverse Primer: 5'-GTAGGAGTTTCCTGCCTCTGTC-3' Tm: 59.258
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 132, 138
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr5:78702469-78702515

Rank: 20	Homology Score: 13	RVD Match Score: 2.861	Left Half-Site RVD Score: 5.028	Right Half-Site RVD Score: 5.394
	Genomic Region: Intron	Closest Gene: C14orf166B
Match Type: R24R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TAACT TGCCCCCGACTCATGT          TTTCTTAGATTATCTCTGGCAATC          ACAGGAGGCAGAGGTA AAGCT-3'
	3'-ATTGA ACGGGGGCTGAGTACA          AAAGAATCTAATAGAGACCGTTAG          TGTCCTCCGTCTCCAT TTCGA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 014-077327606
Forward Primer: 5'-GCCTGGTATAGAACAAGCACCTG-3' Tm: 59.148
Reverse Primer: 5'-CCTCCTGTGATTGCCAGAGC-3' Tm: 59.508
Expected PCR Product Length: 368
Expected CelI Cleavage Product Sizes: 179, 189
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr14:77327755-77327810

Rank: 21	Homology Score: 10	RVD Match Score: 2.876	Left Half-Site RVD Score: 5.619	Right Half-Site RVD Score: 4.921
	Genomic Region: Intron	Closest Gene: MED27
Match Type: R27R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TTGTT TACAAATCACTCCTGC        CATCAACCTCATTTTACAGATCAGGAA         ACAGGAGTGAGCTGAA CAACG-3'
	3'-AACAA ATGTTTAGTGAGGACG        GTAGTTGGAGTAAAATGTCTAGTCCTT         TGTCCTCACTCGACTT GTTGC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 009-134936820
Forward Primer: 5'-GCTGGTAAGTGAAGTTGATGTGGC-3' Tm: 59.038
Reverse Primer: 5'-CCAGGAGCAGCTGCGATGAA-3' Tm: 59.508
Expected PCR Product Length: 318
Expected CelI Cleavage Product Sizes: 187, 131
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr9:134936976-134937034

Rank: 22	Homology Score: 13	RVD Match Score: 2.894	Left Half-Site RVD Score: 4.543	Right Half-Site RVD Score: 6.18
	Genomic Region: Intron	Closest Gene: CACNA2D3
Match Type: R11R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CAAGG TGCCCCACACTCCTGT                GGTCTGGAAGG                 AATGGAGCCATGTGCA CACTT-3'
	3'-GTTCC ACGGGGTGTGAGGACA                CCAGACCTTCC                 TTACCTCGGTACACGT GTGAA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 003-054731501
Forward Primer: 5'-CCTACAGTTTCCAAGGACAAGCAG-3' Tm: 59.038
Reverse Primer: 5'-CCCAGCAGTAGCTTTAGCATCC-3' Tm: 59.258
Expected PCR Product Length: 320
Expected CelI Cleavage Product Sizes: 160, 160
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr3:54731638-54731680

Rank: 23	Homology Score: 5	RVD Match Score: 2.895	Left Half-Site RVD Score: 5.346	Right Half-Site RVD Score: 7.658
	Genomic Region: Intergenic	Closest Gene: SH3BP2
Match Type: R21L
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CCCTC TGCCCCTCCCTCCAGT           GCTGGGCTGTGTGGCCAGGGG      ACTGGACTGTGTGGCAGGATGA CTCCA-3'
	3'-GGGAG ACGGGGAGGGAGGTCA           CGACCCGACACACCGGTCCCC      TGACCTGACACACCGTCCTACT GAGGT-5'
	                                                               TGACGGGACACCCCGTTCCACT
	                                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 004-002790263
Forward Primer: 5'-ATGCAGAGATGGCCCAGAGC-3' Tm: 59.508
Reverse Primer: 5'-TGAGTAGGGCCAAGGCCACA-3' Tm: 59.508
Expected PCR Product Length: 375
Expected CelI Cleavage Product Sizes: 237, 138
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr4:2790469-2790527

Rank: 24	Homology Score: 10	RVD Match Score: 2.897	Left Half-Site RVD Score: 6.631	Right Half-Site RVD Score: 4.189
	Genomic Region: Intron	Closest Gene: POTEF
Match Type: R29R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-AGGAT TGCAGCCAGCTCCTGC       CCCGGTGCAGTGTATACAGTGTATACTGC        ACAGGTGTTGGGTGCA CCAAA-3'
	3'-TCCTA ACGTCGGTCGAGGACG       GGGCCACGTCACATATGTCACATATGACG        TGTCCACAACCCACGT GGTTT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Warning! Primer pair was generated using suboptimal parameters. 
Primer Index: 002-130879063
Forward Primer: 5'-AAGACGCCCACCGAGCCCA-3' Tm: 62.008
Reverse Primer: 5'-GCTAATGGGTGCAAAGTTTCTTTTAAGGAGCATA-3' Tm: 59.668
Expected PCR Product Length: 450
Expected CelI Cleavage Product Sizes: 144, 306
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr2:130879175-130879235

Rank: 25	Homology Score: 10	RVD Match Score: 2.897	Left Half-Site RVD Score: 4.189	Right Half-Site RVD Score: 6.631
	Genomic Region: Intergenic	Closest Gene: POTEE
Match Type: R29R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TTTGG TGCACCCAACACCTGT       GCAGTATACACTGTATACACTGCACCGGG        GCAGGAGCTGGCTGCA ATCCT-3'
	3'-AAACC ACGTGGGTTGTGGACA       CGTCATATGTGACATATGTGACGTGGCCC        CGTCCTCGACCGACGT TAGGA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Warning! Primer pair was generated using suboptimal parameters. 
Primer Index: 002-131974522
Forward Primer: 5'-GCTAATGGGTGCAAAGTTTCTTTTAAGGAGCATA-3' Tm: 59.668
Reverse Primer: 5'-AAGACGCCCACCGAGCCCA-3' Tm: 62.008
Expected PCR Product Length: 450
Expected CelI Cleavage Product Sizes: 309, 141
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr2:131974799-131974859

Rank: 26	Homology Score: 8	RVD Match Score: 2.909	Left Half-Site RVD Score: 6.147	Right Half-Site RVD Score: 4.67
	Genomic Region: Intron	Closest Gene: MYBPHL
Match Type: R30R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-ATGTT TTCACATGCTTCCTGT       GATACTCAGTCCCAGAGCTCTCTGTAAGGA       AGAGGAGTTAGGAGGA AGAAA-3'
	3'-TACAA AAGTGTACGAAGGACA       CTATGAGTCAGGGTCTCGAGAGACATTCCT       TCTCCTCAATCCTCCT TCTTT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 001-109847447
Forward Primer: 5'-GCAGGCTTGCCAAACTAAAAGCTC-3' Tm: 59.038
Reverse Primer: 5'-TCAGAGAGCCAACAGAGCCC-3' Tm: 59.508
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 133, 137
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr1:109847547-109847608

Rank: 27	Homology Score: 10	RVD Match Score: 2.91	Left Half-Site RVD Score: 6.642	Right Half-Site RVD Score: 4.262
	Genomic Region: Intergenic	Closest Gene: NDST3
Match Type: R26R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TTACA TGCATCTATCTCATTT         AATGCTTTTAACAATGCTACAACTAA         AAAGTAGTCAGGTGTA GACTG-3'
	3'-AATGT ACGTAGATAGAGTAAA         TTACGAAAATTGTTACGATGTTGATT         TTTCATCAGTCCACAT CTGAC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Warning! Primer pair was generated using suboptimal parameters. 
Primer Index: 004-118702135
Forward Primer: 5'-GCAGGGACACTGGTGTTATGTCT-3' Tm: 59.148
Reverse Primer: 5'-GATAGTAACTTATTTAGAATTGAGGTTGTGTGATGGAG-3' Tm: 59.358
Expected PCR Product Length: 429
Expected CelI Cleavage Product Sizes: 293, 136
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr4:118702397-118702454

Rank: 28	Homology Score: 5	RVD Match Score: 2.911	Left Half-Site RVD Score: 4.705	Right Half-Site RVD Score: 6.128
	Genomic Region: Intron	Closest Gene: FHIT
Match Type: R20R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CTGCT TACACCTGTCTCCTAA            TAAAATCAACTGCCAATCAC            AGGGGAGGCTGGGGCA TTTTA-3'
	3'-GACGA ATGTGGACAGAGGATT            ATTTTAGTTGACGGTTAGTG            TCCCCTCCGACCCCGT AAAAT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 003-060011649
Forward Primer: 5'-GTACTGCCAGAGTGTCAAGAAAGC-3' Tm: 59.038
Reverse Primer: 5'-TAGGAAGAGGACAGTAGGCTAGG-3' Tm: 59.148
Expected PCR Product Length: 286
Expected CelI Cleavage Product Sizes: 156, 130
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr3:60011777-60011828

Rank: 29	Homology Score: 8	RVD Match Score: 2.913	Left Half-Site RVD Score: 4.624	Right Half-Site RVD Score: 6.232
	Genomic Region: Intergenic	Closest Gene: PISRT1
Match Type: R11R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GTGAT TACCCCTGACTCCACT                CCTGGATGAGG                 TCAGGGGTCATCTGCA CAGAC-3'
	3'-CACTA ATGGGGACTGAGGTGA                GGACCTACTCC                 AGTCCCCAGTAGACGT GTCTG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 003-138953648
Forward Primer: 5'-CTTGGTGTCTGAGTGGCAAAACTG-3' Tm: 59.038
Reverse Primer: 5'-TATGGGCCTGTTTTACACGGGCCT-3' Tm: 60.908
Expected PCR Product Length: 281
Expected CelI Cleavage Product Sizes: 148, 133
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr3:138953773-138953815

Rank: 30	Homology Score: 10	RVD Match Score: 2.918	Left Half-Site RVD Score: 5.228	Right Half-Site RVD Score: 5.612
	Genomic Region: Intergenic	Closest Gene: DHRS2
Match Type: R10R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TCTCC TTCACCTGACTCTAGT                 TGTTGCTGGA                 ACAGGACTTTGTTGTA TATTC-3'
	3'-AGAGG AAGTGGACTGAGATCA                 ACAACGACCT                 TGTCCTGAAACAACAT ATAAG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 014-024242484
Forward Primer: 5'-CAAGTCTTCCTCAGCCATCAGACA-3' Tm: 59.038
Reverse Primer: 5'-CCAGACACATCAACAGAGTGGAAG-3' Tm: 59.038
Expected PCR Product Length: 298
Expected CelI Cleavage Product Sizes: 138, 160
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr14:24242599-24242640

Rank: 31	Homology Score: 5	RVD Match Score: 2.919	Left Half-Site RVD Score: 5.467	Right Half-Site RVD Score: 5.377
	Genomic Region: Exon	Closest Gene: LELP1
Match Type: R24R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TAACA TCCACCTCACTCAACT          GCTTGTCAAGTTCACCACCAACAC          AGAGGGGTAAGTTGCA TTTGG-3'
	3'-ATTGT AGGTGGAGTGAGTTGA          CGAACAGTTCAAGTGGTGGTTGTG          TCTCCCCATTCAACGT AAACC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 001-153175860
Forward Primer: 5'-CTCATAAGTGGAGGTCATTTCCCC-3' Tm: 59.038
Reverse Primer: 5'-GCACACACCAGTTCTTACCACTGA-3' Tm: 59.038
Expected PCR Product Length: 300
Expected CelI Cleavage Product Sizes: 132, 168
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr1:153175962-153176017

Rank: 32	Homology Score: 5	RVD Match Score: 2.923	Left Half-Site RVD Score: 5.84	Right Half-Site RVD Score: 5.046
	Genomic Region: Intergenic	Closest Gene: TMEM185A
Match Type: R27R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TTTCA TTCACCTCATTCATAT        CATCCCCACCAAAAGTATAGCCATTTC         AGTGGAGTCAGCTGTA CACCA-3'
	3'-AAAGT AAGTGGAGTAAGTATA        GTAGGGGTGGTTTTCATATCGGTAAAG         TCACCTCAGTCGACAT GTGGT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 00X-148727617
Forward Primer: 5'-GAGTCTTCCATAGCTGAGTGCAAC-3' Tm: 59.038
Reverse Primer: 5'-ACCCAACATCTGCCATCTGCAAG-3' Tm: 59.148
Expected PCR Product Length: 271
Expected CelI Cleavage Product Sizes: 140, 131
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chrX:148727726-148727784

Rank: 33	Homology Score: 2	RVD Match Score: 2.924	Left Half-Site RVD Score: 5.753	Right Half-Site RVD Score: 5.134
	Genomic Region: Intergenic	Closest Gene: ODZ2
Match Type: R18R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TTTAT TAAACCGCACTCCTTT             GGTGCTTCCTCTGGTGGC             AGAGGAGTGGGGTTTA ATAAA-3'
	3'-AAATA ATTTGGCGTGAGGAAA             CCACGAAGGAGACCACCG             TCTCCTCACCCCAAAT TATTT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 005-166596052
Forward Primer: 5'-CGTTGCCCAGCTTGATGCCT-3' Tm: 59.508
Reverse Primer: 5'-GGAAGTTAGGCCCTAGGACC-3' Tm: 59.508
Expected PCR Product Length: 388
Expected CelI Cleavage Product Sizes: 132, 256
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr5:166596157-166596206

Rank: 34	Homology Score: 10	RVD Match Score: 2.925	Left Half-Site RVD Score: 5.776	Right Half-Site RVD Score: 5.124
	Genomic Region: Exon	Closest Gene: KIF20A
Match Type: R14R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CATCT TACACCCCTCTCCTTT               GGCCCCAGATCTCA               ACTGGATTCATGTGCA AGATG-3'
	3'-GTAGA ATGTGGGGAGAGGAAA               CCGGGGTCTAGAGT               TGACCTAAGTACACGT TCTAC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 005-137519020
Forward Primer: 5'-GCGAGGATCAAAATGGCAATCCC-3' Tm: 59.148
Reverse Primer: 5'-GGCAGAGGACTAATGTTTCCTGG-3' Tm: 59.148
Expected PCR Product Length: 310
Expected CelI Cleavage Product Sizes: 140, 170
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr5:137519135-137519180

Rank: 35	Homology Score: 10	RVD Match Score: 2.926	Left Half-Site RVD Score: 4.264	Right Half-Site RVD Score: 6.778
	Genomic Region: Intron	Closest Gene: TGFBR2
Match Type: R17R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-ATTCC TCCACCTGAGTCCTAT             TGAGGTGCTCCCCATTA              TAAGGAGTCCTGTGAA TCAAA-3'
	3'-TAAGG AGGTGGACTCAGGATA             ACTCCACGAGGGGTAAT              ATTCCTCAGGACACTT AGTTT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 003-030699112
Forward Primer: 5'-CTGGCACAGAGTGAGCACTTAATG-3' Tm: 59.038
Reverse Primer: 5'-GGTCGTCATGAGGAGAACACCTT-3' Tm: 59.148
Expected PCR Product Length: 327
Expected CelI Cleavage Product Sizes: 172, 155
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr3:30699258-30699306

Rank: 36	Homology Score: 8	RVD Match Score: 2.932	Left Half-Site RVD Score: 5.739	Right Half-Site RVD Score: 5.205
	Genomic Region: Intergenic	Closest Gene: TEAD1
Match Type: R20R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-ATAAC TCCTCCCGACCCCTGT            CCTCAGCATCACCATGGGGT            TCATGATTCAGGTGTA TGCAG-3'
	3'-TATTG AGGAGGGCTGGGGACA            GGAGTCGTAGTGGTACCCCA            AGTACTAAGTCCACAT ACGTC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 011-012966658
Forward Primer: 5'-TGAGGGATAGAGCAGAGGATGG-3' Tm: 59.258
Reverse Primer: 5'-GGGGGAATGATGTCACTGGAAC-3' Tm: 59.258
Expected PCR Product Length: 295
Expected CelI Cleavage Product Sizes: 131, 164
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr11:12966761-12966812

Rank: 37	Homology Score: 10	RVD Match Score: 2.935	Left Half-Site RVD Score: 5.124	Right Half-Site RVD Score: 5.849
	Genomic Region: Intron	Closest Gene: USP43
Match Type: R21R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TCATG TGAAACTGACTCCTGA           GAAAATGTGGGCAGGAATGAT            TTAGCAGGCAGGTGGA AGGTG-3'
	3'-AGTAC ACTTTGACTGAGGACT           CTTTTACACCCGTCCTTACTA            AATCGTCCGTCCACCT TCCAC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 017-009569404
Forward Primer: 5'-GGGAATGAAAAGGACAGCAGGAC-3' Tm: 59.148
Reverse Primer: 5'-CGGCCCTGCGCGTTATTTCT-3' Tm: 59.508
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 135, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr17:9569507-9569559

Rank: 38	Homology Score: 5	RVD Match Score: 2.935	Left Half-Site RVD Score: 5.678	Right Half-Site RVD Score: 5.286
	Genomic Region: Intergenic	Closest Gene: AKIRIN2
Match Type: R25R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CTGTG TGCACAGAACTCCTGG         GGGGGAGATGGAATAAGGAAAGCCA          ATGTGATTCAGGTGTA GTGAG-3'
	3'-GACAC ACGTGTCTTGAGGACC         CCCCCTCTACCTTATTCCTTTCGGT          TACACTAAGTCCACAT CACTC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 006-088451104
Forward Primer: 5'-GATCTCCTTGAAGGCACATGCAG-3' Tm: 59.148
Reverse Primer: 5'-GGCCTGGGAAACATAATGACACC-3' Tm: 59.148
Expected PCR Product Length: 325
Expected CelI Cleavage Product Sizes: 130, 195
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr6:88451204-88451260

Rank: 39	Homology Score: 8	RVD Match Score: 2.942	Left Half-Site RVD Score: 6.157	Right Half-Site RVD Score: 4.892
	Genomic Region: Intron	Closest Gene: RPL18A
Match Type: R30R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TAGGG TGGCCCTGCCTCCTGC       TTCCCGAATCTGTGGTCACTAGATGTAGGA       AAGGGAGTGAGGTGGA TCTCC-3'
	3'-ATCCC ACCGGGACGGAGGACG       AAGGGCTTAGACACCAGTGATCTACATCCT       TTCCCTCACTCCACCT AGAGG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 019-017971876
Forward Primer: 5'-CTCAATTAATCCTCCCTGGAGACC-3' Tm: 59.038
Reverse Primer: 5'-ATTCGCATGCGGTAGAGGGG-3' Tm: 59.508
Expected PCR Product Length: 303
Expected CelI Cleavage Product Sizes: 143, 160
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr19:17971986-17972047

Rank: 40	Homology Score: 5	RVD Match Score: 2.945	Left Half-Site RVD Score: 4.652	Right Half-Site RVD Score: 6.456
	Genomic Region: Intergenic	Closest Gene: CNR2
Match Type: R19R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CAGCC TACATCAGACTCCTAT            CTGGGCTGGAATTGGAGAC             CCAGGGGTGAGCTGGA AGAGA-3'
	3'-GTCGG ATGTAGTCTGAGGATA            GACCCGACCTTAACCTCTG             GGTCCCCACTCGACCT TCTCT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 001-024242432
Forward Primer: 5'-TCCCAAGAGCAAGACATTGGCAG-3' Tm: 59.148
Reverse Primer: 5'-GCAATGGAGGACCAGGCGTT-3' Tm: 59.508
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 135, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr1:24242540-24242590

Rank: 41	Homology Score: 5	RVD Match Score: 2.949	Left Half-Site RVD Score: 5.669	Right Half-Site RVD Score: 5.399
	Genomic Region: Intron	Closest Gene: C3orf67
Match Type: R27R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TTTTC TGTCCCTAACTCCTGG        ATTTGACACTGCTTTGTCACAATTTAG         ATGAGAGTGAGGTGGA GCCTT-3'
	3'-AAAAG ACAGGGATTGAGGACC        TAAACTGTGACGAAACAGTGTTAAATC         TACTCTCACTCCACCT CGGAA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 003-058824763
Forward Primer: 5'-GCCAACTGATATATCTGGAGTGGG-3' Tm: 59.038
Reverse Primer: 5'-TGCCAGGCCTTCCACAAGGT-3' Tm: 59.508
Expected PCR Product Length: 404
Expected CelI Cleavage Product Sizes: 273, 131
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr3:58825005-58825063

Rank: 42	Homology Score: 10	RVD Match Score: 2.955	Left Half-Site RVD Score: 4.965	Right Half-Site RVD Score: 6.177
	Genomic Region: Intron	Closest Gene: EIF2B3
Match Type: R12R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-AAAAA TGCAGCTCACACCTGT                GATCCCAGTACT                TTAGGAGGCAGAGGCA GGCAG-3'
	3'-TTTTT ACGTCGAGTGTGGACA                CTAGGGTCATGA                AATCCTCCGTCTCCGT CCGTC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 001-045422945
Forward Primer: 5'-GCCCCCAAACACCACAAAGTC-3' Tm: 59.378
Reverse Primer: 5'-ATTACAGGCACATGCCACCACG-3' Tm: 59.258
Expected PCR Product Length: 370
Expected CelI Cleavage Product Sizes: 240, 130
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr1:45423161-45423204

Rank: 43	Homology Score: 13	RVD Match Score: 2.955	Left Half-Site RVD Score: 4.992	Right Half-Site RVD Score: 6.149
	Genomic Region: Intergenic	Closest Gene: FRG1
Match Type: R14R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GTTTC TACCCCTGACCCCTGC               AGTCTACTCTGAGC               ACAGCAGTCAGAAGCA TTCTT-3'
	3'-CAAAG ATGGGGACTGGGGACG               TCAGATGAGACTCG               TGTCGTCAGTCTTCGT AAGAA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 004-190659833
Forward Primer: 5'-CTGCCTCCAATCCCTCAGAAAATG-3' Tm: 59.038
Reverse Primer: 5'-CTGGTATTGGGTCCAGACCAC-3' Tm: 59.378
Expected PCR Product Length: 308
Expected CelI Cleavage Product Sizes: 178, 130
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr4:190659986-190660031

Rank: 44	Homology Score: 2	RVD Match Score: 2.958	Left Half-Site RVD Score: 5.038	Right Half-Site RVD Score: 6.121
	Genomic Region: Intergenic	Closest Gene: LOC100287010
Match Type: R12R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-AGTTG TCCACCAACCTCCTCT                GGTCAGCAATTA                AGAGGAGGCTGGTAGA CAACC-3'
	3'-TCAAC AGGTGGTTGGAGGAGA                CCAGTCGTTAAT                TCTCCTCCGACCATCT GTTGG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 002-104369295
Forward Primer: 5'-AGGAAGGGAAACTTCACCTCCTG-3' Tm: 59.148
Reverse Primer: 5'-CCACCACCACAGTCAAGATGC-3' Tm: 59.378
Expected PCR Product Length: 360
Expected CelI Cleavage Product Sizes: 131, 229
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr2:104369402-104369445

Rank: 45	Homology Score: 17	RVD Match Score: 2.96	Left Half-Site RVD Score: 4.122	Right Half-Site RVD Score: 7.246
	Genomic Region: Intergenic	Closest Gene: SH3BP4
Match Type: R23R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GCAAT TGCACCTCACTTCTGT          GGAGTGCTTCCTGTGTATCAGGC           ACAGAAGCCCGATGTA TTCTC-3'
	3'-CGTTA ACGTGGAGTGAAGACA          CCTCACGAAGGACACATAGTCCG           TGTCTTCGGGCTACAT AAGAG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 002-235805535
Forward Primer: 5'-ACCTCCACCAGACCACGAATG-3' Tm: 59.378
Reverse Primer: 5'-GACCCTTAGCAGGTCACACTC-3' Tm: 59.378
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 135, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr2:235805641-235805695

Rank: 46	Homology Score: 10	RVD Match Score: 2.962	Left Half-Site RVD Score: 5.124	Right Half-Site RVD Score: 6.063
	Genomic Region: Intron	Closest Gene: USP43
Match Type: R28R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TCATG TGAAACTGACTCCTGA        GAAAATGTGGGCAGGAATGATTTAGCAG        GCAGGTGGAAGGTGGA GAGAT-3'
	3'-AGTAC ACTTTGACTGAGGACT        CTTTTACACCCGTCCTTACTAAATCGTC        CGTCCACCTTCCACCT CTCTA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 017-009569404
Forward Primer: 5'-GGGAATGAAAAGGACAGCAGGAC-3' Tm: 59.148
Reverse Primer: 5'-CGGCCCTGCGCGTTATTTCT-3' Tm: 59.508
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 135, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr17:9569507-9569566

Rank: 47	Homology Score: 10	RVD Match Score: 2.97	Left Half-Site RVD Score: 5.441	Right Half-Site RVD Score: 5.785
	Genomic Region: Intron	Closest Gene: ANK1
Match Type: R15R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TATAA TGAACCTGGCTCCCGT              TCCTTTTATATAAGT               AGGGGAGTGAGGAGGA GGGGA-3'
	3'-ATATT ACTTGGACCGAGGGCA              AGGAAAATATATTCA               TCCCCTCACTCCTCCT CCCCT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 008-041570215
Forward Primer: 5'-GGGTGCAGAGAGATTTGCATGTG-3' Tm: 59.148
Reverse Primer: 5'-TGTCTCCTCCTGTCCCTGCT-3' Tm: 59.508
Expected PCR Product Length: 289
Expected CelI Cleavage Product Sizes: 153, 136
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr8:41570343-41570389

Rank: 48	Homology Score: 5	RVD Match Score: 2.97	Left Half-Site RVD Score: 3.923	Right Half-Site RVD Score: 7.608
	Genomic Region: Intergenic	Closest Gene: PTCHD4
Match Type: R11R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TCAAA TACCCCTCACTCCTAT                TATTAACATTG                 ACAAGAGCATGGTACA TGTAT-3'
	3'-AGTTT ATGGGGAGTGAGGATA                ATAATTGTAAC                 TGTTCTCGTACCATGT ACATA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Warning! Primer pair was generated using suboptimal parameters. 
Primer Index: 006-048618042
Forward Primer: 5'-CCTCCTGTCTTTATTAGTCTAGCAAGAAGTTTGT-3' Tm: 59.668
Reverse Primer: 5'-GTGGTGTCCTGGATAAAATCCTGAAACAGA-3' Tm: 60.058
Expected PCR Product Length: 430
Expected CelI Cleavage Product Sizes: 280, 150
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr6:48618299-48618341

Rank: 49	Homology Score: 13	RVD Match Score: 2.97	Left Half-Site RVD Score: 4.86	Right Half-Site RVD Score: 6.424
	Genomic Region: Intergenic	Closest Gene: FAM69C
Match Type: R26R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CCTAG TTCACTTCACTCCTGT         TATCCACTAAGCTCATTATCACATCG         CCAGAAGTGAGGTCCA TTTGT-3'
	3'-GGATC AAGTGAAGTGAGGACA         ATAGGTGATTCGAGTAATAGTGTAGC         GGTCTTCACTCCAGGT AAACA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 018-072086920
Forward Primer: 5'-GCTGTCACCAGGAAAAACATAAGAGCC-3' Tm: 60.438
Reverse Primer: 5'-AAGCTGCTGAAATGGGCAGAGG-3' Tm: 59.258
Expected PCR Product Length: 289
Expected CelI Cleavage Product Sizes: 159, 130
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr18:72087048-72087105

Rank: 50	Homology Score: 8	RVD Match Score: 2.972	Left Half-Site RVD Score: 6.792	Right Half-Site RVD Score: 4.559
	Genomic Region: Intron	Closest Gene: ATP2B2
Match Type: R19R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GGACA TGCAGCTGACACTGGT            ACTTTCTCCTCTTCTGGAC             AAGGGAGTGAGGTGTA GCCAG-3'
	3'-CCTGT ACGTCGACTGTGACCA            TGAAAGAGGAGAAGACCTG             TTCCCTCACTCCACAT CGGTC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 003-010453381
Forward Primer: 5'-TGTTGCAGACACTGGAACAGAGC-3' Tm: 59.148
Reverse Primer: 5'-CGTGAGAGTGGACTTGCAAGTCTG-3' Tm: 60.908
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 135, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr3:10453489-10453539

Rank: 51	Homology Score: 10	RVD Match Score: 2.972	Left Half-Site RVD Score: 5.472	Right Half-Site RVD Score: 5.77
	Genomic Region: Intron	Closest Gene: PIBF1
Match Type: R23R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-AAATT TACCCCTCTCTCCTCT          GAATTTAGAACTATTAAGGAGAA           ACAAGAGTCAGTTACA TATCT-3'
	3'-TTTAA ATGGGGAGAGAGGAGA          CTTAAATCTTGATAATTCCTCTT           TGTTCTCAGTCAATGT ATAGA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 013-073489472
Forward Primer: 5'-GCGCTCTGCTTCTAAGACACATTG-3' Tm: 59.038
Reverse Primer: 5'-CACATGGAAGAGATGATACATGCCATTACC-3' Tm: 60.058
Expected PCR Product Length: 386
Expected CelI Cleavage Product Sizes: 243, 143
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr13:73489686-73489740

Rank: 52	Homology Score: 10	RVD Match Score: 2.973	Left Half-Site RVD Score: 6.646	Right Half-Site RVD Score: 4.688
	Genomic Region: Intron	Closest Gene: CDC42BPA
Match Type: R26R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-ACTTC TGCAGCTGACTCAACA         ATCTGGGTTTCAACCACCCTTCCTCC         ATAGGATTCAGATGCA TGCCA-3'
	3'-TGAAG ACGTCGACTGAGTTGT         TAGACCCAAAGTTGGTGGGAAGGAGG         TATCCTAAGTCTACGT ACGGT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 001-227379954
Forward Primer: 5'-GAGATCACTCTCACCACCCAAAAC-3' Tm: 59.038
Reverse Primer: 5'-GGAAACAGCAGAACGTTGTTGGTG-3' Tm: 59.038
Expected PCR Product Length: 383
Expected CelI Cleavage Product Sizes: 190, 193
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr1:227380113-227380170

Rank: 53	Homology Score: 5	RVD Match Score: 2.973	Left Half-Site RVD Score: 5.701	Right Half-Site RVD Score: 5.551
	Genomic Region: Intergenic	Closest Gene: NKX6-1
Match Type: R29R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CAATT TTAACCTGACTCCATT       TTTCACTTAGCATTGTTCTAAACAATAAA        ACATGAGTTTAGTGTA TTTAT-3'
	3'-GTTAA AATTGGACTGAGGTAA       AAAGTGAATCGTAACAAGATTTGTTATTT        TGTACTCAAATCACAT AAATA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 004-085199195
Forward Primer: 5'-CAGTTCTACTTCAGGGAAGGGG-3' Tm: 59.258
Reverse Primer: 5'-GGATACAAGTGGAAAAGCACAGCC-3' Tm: 59.038
Expected PCR Product Length: 364
Expected CelI Cleavage Product Sizes: 196, 168
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr4:85199359-85199419

Rank: 54	Homology Score: 8	RVD Match Score: 2.974	Left Half-Site RVD Score: 6.288	Right Half-Site RVD Score: 5.006
	Genomic Region: Intergenic	Closest Gene: USP22
Match Type: R10R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TACAA TGCATATGACAACTGT                 TACACAAGGG                 ACAGGAAGGAGGTGTA AATGG-3'
	3'-ATGTT ACGTATACTGTTGACA                 ATGTGTTCCC                 TGTCCTTCCTCCACAT TTACC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 017-020954067
Forward Primer: 5'-CGGGAAAAAGTACCAGATGGAGAC-3' Tm: 59.038
Reverse Primer: 5'-GACAGAGTGAGACCCTGTCTCAA-3' Tm: 59.148
Expected PCR Product Length: 346
Expected CelI Cleavage Product Sizes: 153, 193
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr17:20954197-20954238

Rank: 55	Homology Score: 2	RVD Match Score: 2.975	Left Half-Site RVD Score: 5.516	Right Half-Site RVD Score: 5.751
	Genomic Region: Intergenic	Closest Gene: GBE1
Match Type: R18R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-AAACC TAGACCTACCACCTGT             GTTCCACCAAATTTATTG             GTAGGAGGAAGGGGCA AAGGG-3'
	3'-TTTGG ATCTGGATGGTGGACA             CAAGGTGGTTTAAATAAC             CATCCTCCTTCCCCGT TTCCC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 003-082432463
Forward Primer: 5'-GACAGGGAAGAAACTCTGCAAACC-3' Tm: 59.038
Reverse Primer: 5'-GCACGGATATGCAATAAACTTCTGTTTCCC-3' Tm: 60.058
Expected PCR Product Length: 395
Expected CelI Cleavage Product Sizes: 134, 261
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr3:82432570-82432619

Rank: 56	Homology Score: 2	RVD Match Score: 2.977	Left Half-Site RVD Score: 5.502	Right Half-Site RVD Score: 5.78
	Genomic Region: Intergenic	Closest Gene: KLF8
Match Type: R24R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TTTAT TCAACCAAACACCTGT          ATTTTTCCCCAGTATTTCACTAAT          ATAAAAGTGAGGTGGA TGACT-3'
	3'-AAATA AGTTGGTTTGTGGACA          TAAAAAGGGGTCATAAAGTGATTA          TATTTTCACTCCACCT ACTGA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 00X-056339296
Forward Primer: 5'-GCAAGAATATTGGCAATACTGTCCCATGTC-3' Tm: 60.058
Reverse Primer: 5'-GCAGAGGCCTAGTCTGAAGC-3' Tm: 59.508
Expected PCR Product Length: 396
Expected CelI Cleavage Product Sizes: 132, 264
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chrX:56339398-56339453

Rank: 57	Homology Score: 2	RVD Match Score: 2.977	Left Half-Site RVD Score: 5.78	Right Half-Site RVD Score: 5.502
	Genomic Region: Intergenic	Closest Gene: ZC3H12C
Match Type: R24R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-AGTCA TCCACCTCACTTTTAT          ATTAGTGAAATACTGGGGAAAATT          ACAGGTGTTTGGTTGA ATAAA-3'
	3'-TCAGT AGGTGGAGTGAAAATA          TAATCACTTTATGACCCCTTTTAA          TGTCCACAAACCAACT TATTT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Warning! Primer pair was generated using suboptimal parameters. 
Primer Index: 011-109690484
Forward Primer: 5'-GCAGAGGCCTAGTCTGAAGC-3' Tm: 59.508
Reverse Primer: 5'-AAGCAAGAATATTGGCAATACTGTCCCATGTC-3' Tm: 59.848
Expected PCR Product Length: 398
Expected CelI Cleavage Product Sizes: 268, 130
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr11:109690722-109690777

Rank: 58	Homology Score: 8	RVD Match Score: 2.979	Left Half-Site RVD Score: 6.098	Right Half-Site RVD Score: 5.216
	Genomic Region: Intergenic	Closest Gene: KHDRBS2
Match Type: R18R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-AGCAC TGCAACTCATTCCTGG             TGCCCAGTGGCTCCTAGG             AAAGGGGTGAGGTGAA CAGGT-3'
	3'-TCGTG ACGTTGAGTAAGGACC             ACGGGTCACCGAGGATCC             TTTCCCCACTCCACTT GTCCA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 006-063449762
Forward Primer: 5'-TCTGAGGAGACCTTCAGAGGG-3' Tm: 59.378
Reverse Primer: 5'-ACCACCTCTTGAAGCAGCTCTC-3' Tm: 59.258
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 135, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr6:63449870-63449919

Rank: 59	Homology Score: 17	RVD Match Score: 2.98	Left Half-Site RVD Score: 4.129	Right Half-Site RVD Score: 7.408
	Genomic Region: Intron	Closest Gene: CARD11
Match Type: R17R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GGGGG TGCACCTGCCTCCTGC             CTGCACCCTGGGAGCTT              GCAGGATGCAGAAGCA TGGGC-3'
	3'-CCCCC ACGTGGACGGAGGACG             GACGTGGGACCCTCGAA              CGTCCTACGTCTTCGT ACCCG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 007-002962408
Forward Primer: 5'-ACAGCGTTTGTCTCCTGGCC-3' Tm: 59.508
Reverse Primer: 5'-GCCCCTAAGCCTTGCATTCAG-3' Tm: 59.378
Expected PCR Product Length: 298
Expected CelI Cleavage Product Sizes: 167, 131
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr7:2962549-2962597

Rank: 60	Homology Score: 8	RVD Match Score: 2.981	Left Half-Site RVD Score: 5.281	Right Half-Site RVD Score: 6.042
	Genomic Region: Intron	Closest Gene: ZFPM1
Match Type: R29R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-ACGCC TTCACCTGCCTCCTCA       AGTCAGAGCTGTGAGCCAGCCTGCCAGGG        CCAGGGCTCAGGTGGA GAAAC-3'
	3'-TGCGG AAGTGGACGGAGGAGT       TCAGTCTCGACACTCGGTCGGACGGTCCC        GGTCCCGAGTCCACCT CTTTG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 016-088575852
Forward Primer: 5'-CCATTAGGCACACAGGGAGG-3' Tm: 59.508
Reverse Primer: 5'-CTGCCTCCTGAACCCACTCT-3' Tm: 59.508
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 135, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr16:88575955-88576015

Rank: 61	Homology Score: 2	RVD Match Score: 2.982	Left Half-Site RVD Score: 5.87	Right Half-Site RVD Score: 5.447
	Genomic Region: Intergenic	Closest Gene: VIPR2
Match Type: R27R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GTTTA TGCACCTAACAACAGA        GCTTGAAAATACATGAAGCAAATACTG         ATAGAAGTGAGATGTA ATCTA-3'
	3'-CAAAT ACGTGGATTGTTGTCT        CGAACTTTTATGTACTTCGTTTATGAC         TATCTTCACTCTACAT TAGAT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 007-158980941
Forward Primer: 5'-GCCCAACTACATGCTGGGTC-3' Tm: 59.508
Reverse Primer: 5'-GTGGCCTATCTTGATGAATGTCCC-3' Tm: 59.038
Expected PCR Product Length: 422
Expected CelI Cleavage Product Sizes: 200, 222
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr7:158981110-158981168

Rank: 62	Homology Score: 10	RVD Match Score: 2.983	Left Half-Site RVD Score: 7.259	Right Half-Site RVD Score: 4.262
	Genomic Region: Intron	Closest Gene: COL25A1
Match Type: R11R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TGCAA TGCAGCGGGCTCCCCT                TACACACCTTT                 ACAGGAGTTAGTTGAA TATCA-3'
	3'-ACGTT ACGTCGCCCGAGGGGA                ATGTGTGGAAA                 TGTCCTCAATCAACTT ATAGT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 004-110007835
Forward Primer: 5'-GACTGCTGGGAATTGCTCTTTGAC-3' Tm: 59.038
Reverse Primer: 5'-TCCCTCAACTTCCACTGGGC-3' Tm: 59.508
Expected PCR Product Length: 290
Expected CelI Cleavage Product Sizes: 157, 133
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr4:110007969-110008011

Rank: 63	Homology Score: 10	RVD Match Score: 2.983	Left Half-Site RVD Score: 6.477	Right Half-Site RVD Score: 4.904
	Genomic Region: Intergenic	Closest Gene: PTGER4P2
Match Type: R18R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GGTAA TCCACCTGCCTCGGCT             TCCCAAAGTGTTGGGGTT             ACAGGTGTGAGGTACA ACGCC-3'
	3'-CCATT AGGTGGACGGAGCCGA             AGGGTTTCACAACCCCAA             TGTCCACACTCCATGT TGCGG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 009-066282880
Forward Primer: 5'-ACTACAGGTGCACACCACCATG-3' Tm: 59.258
Reverse Primer: 5'-GCCTTCCCCAAGTTTGCATTTTCG-3' Tm: 59.038
Expected PCR Product Length: 301
Expected CelI Cleavage Product Sizes: 131, 170
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr9:66282984-66283033

Rank: 64	Homology Score: 17	RVD Match Score: 2.985	Left Half-Site RVD Score: 6.923	Right Half-Site RVD Score: 4.543
	Genomic Region: Intergenic	Closest Gene: SLC2A10
Match Type: R24R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CCCCT TCCTCCAGCTTCCTGT          AGGTTCAACTAGGGGGAAGCCCCA          GCAGGAGACAGGTGCA CAGTG-3'
	3'-GGGGA AGGAGGTCGAAGGACA          TCCAAGTTGATCCCCCTTCGGGGT          CGTCCTCTGTCCACGT GTCAC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 020-045428486
Forward Primer: 5'-GTCTCACTGAGACCTAGGCG-3' Tm: 59.508
Reverse Primer: 5'-TGGGCCTGGGGAACCAACAA-3' Tm: 59.508
Expected PCR Product Length: 268
Expected CelI Cleavage Product Sizes: 133, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr20:45428589-45428644

Rank: 65	Homology Score: 2	RVD Match Score: 2.987	Left Half-Site RVD Score: 5.939	Right Half-Site RVD Score: 7.469
	Genomic Region: Intergenic	Closest Gene: TICRR
Match Type: L30R
	           Left Nuclease
	         TCACCTTGCCCCACAGGGCAGT
	5'-GCCCG TCACCTTGCCCAACCCAACAGT CCATTCTCAGCCTTCATTTTATCCCACCTC       ACAGCAGCCTGTGGCA GAGGG-3'
	3'-CGGGC AGTGGAACGGGTTGGGTTGTCA GGTAAGAGTCGGAAGTAAAATAGGGTGGAG       TGTCGTCGGACACCGT CTCCC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 015-090111045
Forward Primer: 5'-GCCTTCCTCTCCTCATACCC-3' Tm: 59.508
Reverse Primer: 5'-TACAGGTGTGAGCCACCACG-3' Tm: 59.508
Expected PCR Product Length: 273
Expected CelI Cleavage Product Sizes: 131, 142
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr15:90111140-90111207

Rank: 66	Homology Score: 10	RVD Match Score: 2.987	Left Half-Site RVD Score: 5.715	Right Half-Site RVD Score: 5.64
	Genomic Region: Exon	Closest Gene: KIAA1244
Match Type: R19R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CATTG TGTAGCCGACTCCTGT            TCTACTCTCCCACCAAATA             ACAGTAGTGAGGGTTA GAGTC-3'
	3'-GTAAC ACATCGGCTGAGGACA            AGATGAGAGGGTGGTTTAT             TGTCATCACTCCCAAT CTCAG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 006-138657515
Forward Primer: 5'-GTTCCCGTGCATCAGTCAGC-3' Tm: 59.508
Reverse Primer: 5'-GCTGATCAGTGGTTGGAAAGGC-3' Tm: 59.258
Expected PCR Product Length: 272
Expected CelI Cleavage Product Sizes: 131, 141
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr6:138657619-138657669

Rank: 67	Homology Score: 13	RVD Match Score: 2.988	Left Half-Site RVD Score: 6.01	Right Half-Site RVD Score: 5.362
	Genomic Region: Intergenic	Closest Gene: ATP8B1
Match Type: R11R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-AGGGC TGCACGTGACTTCTGG                GGCAAAGGTAA                 GTAGGAGAAAGGTGCA CCTTC-3'
	3'-TCCCG ACGTGCACTGAAGACC                CCGTTTCCATT                 CATCCTCTTTCCACGT GGAAG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 018-055555293
Forward Primer: 5'-TCTGGGAAGAAGCTGCCAGG-3' Tm: 59.508
Reverse Primer: 5'-GGCTATGCTACCTGCTGTAAAACC-3' Tm: 59.038
Expected PCR Product Length: 342
Expected CelI Cleavage Product Sizes: 134, 208
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr18:55555404-55555446

Rank: 68	Homology Score: 5	RVD Match Score: 2.99	Left Half-Site RVD Score: 5.538	Right Half-Site RVD Score: 5.84
	Genomic Region: Intron	Closest Gene: RNF128
Match Type: R13R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CGATG TCCAACTGACTCATGA               TCTATGTGGAAAG                AAAGTAGTGAAGTGTA GGTGT-3'
	3'-GCTAC AGGTTGACTGAGTACT               AGATACACCTTTC                TTTCATCACTTCACAT CCACA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 00X-106025404
Forward Primer: 5'-CTTTCCCCAACTGTGTGATACAGC-3' Tm: 59.038
Reverse Primer: 5'-CTGGTACTGGAACTCTGCTCTTCA-3' Tm: 59.038
Expected PCR Product Length: 380
Expected CelI Cleavage Product Sizes: 191, 189
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chrX:106025571-106025615

Rank: 69	Homology Score: 2	RVD Match Score: 2.992	Left Half-Site RVD Score: 5.399	Right Half-Site RVD Score: 6.001
	Genomic Region: Intergenic	Closest Gene: HSP90AA4P
Match Type: R10R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CCCCG TCCACCCCATTCCTAT                 CTGTGTTATT                 ACAGGATTGAATTGTA ATTTG-3'
	3'-GGGGC AGGTGGGGTAAGGATA                 GACACAATAA                 TGTCCTAACTTAACAT TAAAC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 004-190407488
Forward Primer: 5'-CCTGGGGGAACTTTTTCAGTCCAT-3' Tm: 59.038
Reverse Primer: 5'-GGTTTCACATGCCCTGAATACTGC-3' Tm: 59.038
Expected PCR Product Length: 407
Expected CelI Cleavage Product Sizes: 211, 196
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr4:190407676-190407717

Rank: 70	Homology Score: 13	RVD Match Score: 2.993	Left Half-Site RVD Score: 4.632	Right Half-Site RVD Score: 6.878
	Genomic Region: Intergenic	Closest Gene: ZNF812
Match Type: R29R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TTTTG TGCACCCAACTGCTGT       GGAGTGTCCCAGCAGAACGTCGTCCATGT        ACAGGAGCAAGACGCA GCCTA-3'
	3'-AAAAC ACGTGGGTTGACGACA       CCTCACAGGGTCGTCTTGCAGCAGGTACA        TGTCCTCGTTCTGCGT CGGAT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 019-009837811
Forward Primer: 5'-AATCCCAGGTAGCGTACGTGC-3' Tm: 59.378
Reverse Primer: 5'-TCAGTACACATGGACCTGGCTTC-3' Tm: 59.148
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 135, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr19:9837914-9837974

Rank: 71	Homology Score: 8	RVD Match Score: 2.994	Left Half-Site RVD Score: 5.695	Right Half-Site RVD Score: 5.716
	Genomic Region: Intergenic	Closest Gene: YIPF5
Match Type: R12R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-ACTGC TTCACCTGTCTCCTCC                CATTATAAATCT                TCAGGAGTCCTGTGGA GGTGG-3'
	3'-TGACG AAGTGGACAGAGGAGG                GTAATATTTAGA                AGTCCTCAGGACACCT CCACC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 005-143474515
Forward Primer: 5'-CCACTCTCCCTCAAATCAACTGC-3' Tm: 59.148
Reverse Primer: 5'-CTGCATTTCTGAGACAGACATGGC-3' Tm: 59.038
Expected PCR Product Length: 351
Expected CelI Cleavage Product Sizes: 202, 149
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr5:143474693-143474736

Rank: 72	Homology Score: 5	RVD Match Score: 2.995	Left Half-Site RVD Score: 5.642	Right Half-Site RVD Score: 5.776
	Genomic Region: Intergenic	Closest Gene: ZPLD1
Match Type: R17R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CCTTC TCCACCTTACTCTTGC             TGACAGGAGGAGATTAG              AGGGGAGTTAGGAGAA GGAAG-3'
	3'-GGAAG AGGTGGAATGAGAACG             ACTGTCCTCCTCTAATC              TCCCCTCAATCCTCTT CCTTC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 003-102226111
Forward Primer: 5'-CCAGCATCATTGAAAGCTGGCAGA-3' Tm: 59.038
Reverse Primer: 5'-CTGTATCCCCATCATGTGCTGAAG-3' Tm: 59.038
Expected PCR Product Length: 413
Expected CelI Cleavage Product Sizes: 268, 145
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr3:102226353-102226401

Rank: 73	Homology Score: 10	RVD Match Score: 2.995	Left Half-Site RVD Score: 6.953	Right Half-Site RVD Score: 4.588
	Genomic Region: Intergenic	Closest Gene: LRP2
Match Type: R13R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GAACG TGCACCAGAATTCAGC               CAAGCTCCCTATC                AAAGGAGTCAGGTCTA TCTTT-3'
	3'-CTTGC ACGTGGTCTTAAGTCG               GTTCGAGGGATAG                TTTCCTCAGTCCAGAT AGAAA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 002-170227380
Forward Primer: 5'-GGCCAGAAGACCAAGATGATTCC-3' Tm: 59.148
Reverse Primer: 5'-GCCAAAAGCCAAATGTAGACTTTGTGCTG-3' Tm: 60.178
Expected PCR Product Length: 403
Expected CelI Cleavage Product Sizes: 259, 144
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr2:170227615-170227659

Rank: 74	Homology Score: 13	RVD Match Score: 2.999	Left Half-Site RVD Score: 6.01	Right Half-Site RVD Score: 5.441
	Genomic Region: Intergenic	Closest Gene: GPC1
Match Type: R29R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GAGCC TTCACCTGATTCCAGC       CGCAAAGCCCTTTCCAGTACGGCCCATTC        ACAGGTTCCAGGTGCA AAGCC-3'
	3'-CTCGG AAGTGGACTAAGGTCG       GCGTTTCGGGAAAGGTCATGCCGGGTAAG        TGTCCAAGGTCCACGT TTCGG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 002-241334168
Forward Primer: 5'-CATGCACCCCTGGCTTGTGT-3' Tm: 59.508
Reverse Primer: 5'-AGCTTTGCACCTGGAACCTGTGA-3' Tm: 59.148
Expected PCR Product Length: 297
Expected CelI Cleavage Product Sizes: 138, 159
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr2:241334274-241334334

Rank: 75	Homology Score: 5	RVD Match Score: 2.999	Left Half-Site RVD Score: 6.265	Right Half-Site RVD Score: 5.205
	Genomic Region: Intergenic	Closest Gene: CENPC1
Match Type: R27R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-AAGCG TGCATCTAAATCTTCT        AAACAATCAGAATTCCAGGTTTCTGGG         ATATTTGTCAGGTGCA AAATG-3'
	3'-TTCGC ACGTAGATTTAGAAGA        TTTGTTAGTCTTAAGGTCCAAAGACCC         TATAAACAGTCCACGT TTTAC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 004-068122758
Forward Primer: 5'-CTTTTGCTTGTTGGTTCGGTGGC-3' Tm: 59.148
Reverse Primer: 5'-GTCCTGATGGAGTCTAAATGGCTG-3' Tm: 59.038
Expected PCR Product Length: 281
Expected CelI Cleavage Product Sizes: 134, 147
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr4:68122861-68122919

Rank: 76	Homology Score: 13	RVD Match Score: 2.999	Left Half-Site RVD Score: 4.632	Right Half-Site RVD Score: 6.931
	Genomic Region: Intergenic	Closest Gene: CTSB
Match Type: R29R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CTTGG TGCACCTGACTGCCAT       GGGGTGTCCCAGCAAAAGGGCATCAATCT        ACTGGAGCCACATGCA GTCTA-3'
	3'-GAACC ACGTGGACTGACGGTA       CCCCACAGGGTCGTTTTCCCGTAGTTAGA        TGACCTCGGTGTACGT CAGAT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 008-011765812
Forward Primer: 5'-ATCCCCAGTAATGTACCTGCTGTC-3' Tm: 59.038
Reverse Primer: 5'-CTCAGTACACTTGCACCCGG-3' Tm: 59.508
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 134, 136
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr8:11765914-11765974

Rank: 77	Homology Score: 10	RVD Match Score: 2.999	Left Half-Site RVD Score: 5.884	Right Half-Site RVD Score: 5.567
	Genomic Region: Intergenic	Closest Gene: FLJ43860
Match Type: R24R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TTTGT TAAACCACATTCCTGT          GAGAGGCAACTTTTCAACTGGAGA          ACAGTACTCATGTGCA GTTCC-3'
	3'-AAACA ATTTGGTGTAAGGACA          CTCTCCGTTGAAAAGTTGACCTCT          TGTCATGAGTACACGT CAAGG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 008-142885698
Forward Primer: 5'-GTGAACTACCTCTCTGGCACTG-3' Tm: 59.258
Reverse Primer: 5'-CTCACCCAAAACTGGAAGCAACC-3' Tm: 59.148
Expected PCR Product Length: 336
Expected CelI Cleavage Product Sizes: 132, 204
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr8:142885800-142885855

Rank: 78	Homology Score: 17	RVD Match Score: 2.999	Left Half-Site RVD Score: 4.607	Right Half-Site RVD Score: 6.963
	Genomic Region: Intron	Closest Gene: CABIN1
Match Type: R10R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GGGGT TGCATCTGAATCCTGT                 GGGTGTGGCA                 GCAGGGGACAGCTGGA AAAGG-3'
	3'-CCCCA ACGTAGACTTAGGACA                 CCCACACCGT                 CGTCCCCTGTCGACCT TTTCC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 022-024555541
Forward Primer: 5'-CACAGCCATTCTGTCTACTCCC-3' Tm: 59.258
Reverse Primer: 5'-TGGCAGGTGGCCAGTGTCTT-3' Tm: 59.508
Expected PCR Product Length: 273
Expected CelI Cleavage Product Sizes: 140, 133
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr22:24555658-24555699

Rank: 79	Homology Score: 10	RVD Match Score: 3	Left Half-Site RVD Score: 4.963	Right Half-Site RVD Score: 6.542
	Genomic Region: Intergenic	Closest Gene: PPARGC1A
Match Type: R13R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-ACAAA TGCACCCTACTCATGT               AAGATGTTATAAC                AGGGGAGACTGGGGCA GGGCA-3'
	3'-TGTTT ACGTGGGATGAGTACA               TTCTACAATATTG                TCCCCTCTGACCCCGT CCCGT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 004-024170953
Forward Primer: 5'-CTGGATGGGATCCCAGAACAGA-3' Tm: 59.258
Reverse Primer: 5'-GAGCCAACATGCTTGGCTGAG-3' Tm: 59.378
Expected PCR Product Length: 276
Expected CelI Cleavage Product Sizes: 140, 136
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr4:24171069-24171113

Rank: 80	Homology Score: 5	RVD Match Score: 3	Left Half-Site RVD Score: 4.957	Right Half-Site RVD Score: 6.555
	Genomic Region: Intron	Closest Gene: TMPRSS4-AS1
Match Type: R11R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-AGTGG TGCCCCTCACTCCTCA                CACAGGTGCTG                 AGAGGAGGGAAGAGCA CCCAG-3'
	3'-TCACC ACGGGGAGTGAGGAGT                GTGTCCACGAC                 TCTCCTCCCTTCTCGT GGGTC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 011-117929965
Forward Primer: 5'-GCTCCGATGTGAGAAGTCTCTAG-3' Tm: 59.148
Reverse Primer: 5'-CCTTCCACACATTCTGCACTCTG-3' Tm: 59.148
Expected PCR Product Length: 274
Expected CelI Cleavage Product Sizes: 133, 141
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr11:117930075-117930117

Rank: 81	Homology Score: 8	RVD Match Score: 3.003	Left Half-Site RVD Score: 4.559	Right Half-Site RVD Score: 7.048
	Genomic Region: Intron	Closest Gene: APOL2
Match Type: R25R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-AGGCC TTCACCACACTCCTAT         GACATTGAATTGCTATTCCTATTGG          ACAGAAGACAGTTCCA AGTCT-3'
	3'-TCCGG AAGTGGTGTGAGGATA         CTGTAACTTAACGATAAGGATAACC          TGTCTTCTGTCAAGGT TCAGA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 022-036626609
Forward Primer: 5'-CTGACTCCAGGCTCTGACAC-3' Tm: 59.508
Reverse Primer: 5'-GCTGGGGCCTTTTTTCTCATCTG-3' Tm: 59.148
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 130, 140
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr22:36626709-36626765

Rank: 82	Homology Score: 5	RVD Match Score: 3.003	Left Half-Site RVD Score: 4.624	Right Half-Site RVD Score: 6.968
	Genomic Region: Intergenic	Closest Gene: ZNF629
Match Type: R19R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GGGAC TGCACCTCCCTCCTAC            TCATTGTGGACAGCAGAGG             AGAAGAGAGAGGAGCA GGAGA-3'
	3'-CCCTG ACGTGGAGGGAGGATG            AGTAACACCTGTCGTCTCC             TCTTCTCTCTCCTCGT CCTCT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 016-030831779
Forward Primer: 5'-CACACACACACACACATGGGC-3' Tm: 59.378
Reverse Primer: 5'-ACTGTCTGGGGAAGGGATGG-3' Tm: 59.508
Expected PCR Product Length: 366
Expected CelI Cleavage Product Sizes: 183, 183
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr16:30831935-30831985

Rank: 83	Homology Score: 2	RVD Match Score: 3.004	Left Half-Site RVD Score: 6.217	Right Half-Site RVD Score: 5.286
	Genomic Region: Exon	Closest Gene: UMODL1
Match Type: R29R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CTCCA TGCACCTCAAATCTCT       CTCTCCTTCCTTTTGTAAGGATGCCAGTC        ATTGGATTTAGGTTCA CCTTA-3'
	3'-GAGGT ACGTGGAGTTTAGAGA       GAGAGGAAGGAAAACATTCCTACGGTCAG        TAACCTAAATCCAAGT GGAAT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 021-043483081
Forward Primer: 5'-TGGAGGCTGCGAGGGAGAAA-3' Tm: 59.508
Reverse Primer: 5'-GCACCCCTGCAAAAGATATGTCC-3' Tm: 59.148
Expected PCR Product Length: 302
Expected CelI Cleavage Product Sizes: 157, 145
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr21:43483206-43483266

Rank: 84	Homology Score: 8	RVD Match Score: 3.006	Left Half-Site RVD Score: 5.848	Right Half-Site RVD Score: 5.649
	Genomic Region: Intergenic	Closest Gene: RGS17
Match Type: R11R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CCTTG TGCAGCTGGCTCCCAT                GATGGCAGCCA                 AATGGAGGCTGGTGCA CTGTG-3'
	3'-GGAAC ACGTCGACCGAGGGTA                CTACCGTCGGT                 TTACCTCCGACCACGT GACAC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 006-153846994
Forward Primer: 5'-GCAAGCTGTCTCATGCTCACTG-3' Tm: 59.258
Reverse Primer: 5'-CAGGAAGGAACTTGTCAGATTCCC-3' Tm: 59.038
Expected PCR Product Length: 366
Expected CelI Cleavage Product Sizes: 229, 137
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr6:153847200-153847242

Rank: 85	Homology Score: 10	RVD Match Score: 3.007	Left Half-Site RVD Score: 5.285	Right Half-Site RVD Score: 6.24
	Genomic Region: Intron	Closest Gene: SH3RF1
Match Type: R14R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GTTTC TGTACCTCACTTCTGT               TTTCTGTGAATCAG               AGAGGATTTCGGTCCA TGAAT-3'
	3'-CAAAG ACATGGAGTGAAGACA               AAAGACACTTAGTC               TCTCCTAAAGCCAGGT ACTTA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 004-170092193
Forward Primer: 5'-GACCTAAAGGTTTCTCCACACATAGTGAAG-3' Tm: 60.058
Reverse Primer: 5'-GACTGTTCTGAGATCTTGGGTGTG-3' Tm: 59.038
Expected PCR Product Length: 350
Expected CelI Cleavage Product Sizes: 192, 158
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr4:170092360-170092405

Rank: 86	Homology Score: 2	RVD Match Score: 3.008	Left Half-Site RVD Score: 6.245	Right Half-Site RVD Score: 5.286
	Genomic Region: Intron	Closest Gene: PCCA
Match Type: R26R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CCATA TGCCCCTCCCTCATGC         CACACCATTTCTCTACTTAACATCTC         ATATTAGTTTGGTGCA TTTGT-3'
	3'-GGTAT ACGGGGAGGGAGTACG         GTGTGGTAAAGAGATGAATTGTAGAG         TATAATCAAACCACGT AAACA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 013-100940227
Forward Primer: 5'-GTGGTAGACCTTCAGAATTTTATGAGGGAG-3' Tm: 60.058
Reverse Primer: 5'-TCACCACTGCACTCCAGCCT-3' Tm: 59.508
Expected PCR Product Length: 387
Expected CelI Cleavage Product Sizes: 250, 137
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr13:100940446-100940503

Rank: 87	Homology Score: 8	RVD Match Score: 3.008	Left Half-Site RVD Score: 5.703	Right Half-Site RVD Score: 5.81
	Genomic Region: Intron	Closest Gene: NPC1L1
Match Type: R21R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CCCTC TTCACCTCAGCCCTGT           GACATAGCCCCCAGGGCTTCT            GCAGGTGGCAGGTTCA TTCTG-3'
	3'-GGGAG AAGTGGAGTCGGGACA           CTGTATCGGGGGTCCCGAAGA            CGTCCACCGTCCAAGT AAGAC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 007-044570049
Forward Primer: 5'-GGAGATGGATGGTGGTGATAGTTG-3' Tm: 59.038
Reverse Primer: 5'-TTAGGCAGTACTGGCCTGGG-3' Tm: 59.508
Expected PCR Product Length: 434
Expected CelI Cleavage Product Sizes: 294, 140
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr7:44570315-44570367

Rank: 88	Homology Score: 5	RVD Match Score: 3.01	Left Half-Site RVD Score: 5.044	Right Half-Site RVD Score: 6.534
	Genomic Region: Exon	Closest Gene: TRIM67
Match Type: R18R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TACCC TGAACCTAACCCCTTT             GAGGGAGCAGCATCCAGG             TCAGGAGAGAAGTGGA AAGTG-3'
	3'-ATGGG ACTTGGATTGGGGAAA             CTCCCTCGTCGTAGGTCC             AGTCCTCTCTTCACCT TTCAC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 001-231353429
Forward Primer: 5'-AAGGCTGTGGAGCCTCCACCAT-3' Tm: 61.288
Reverse Primer: 5'-AATCCCAGCCCCCAAGCTCA-3' Tm: 59.508
Expected PCR Product Length: 279
Expected CelI Cleavage Product Sizes: 131, 148
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr1:231353533-231353582

Rank: 89	Homology Score: 5	RVD Match Score: 3.01	Left Half-Site RVD Score: 6.229	Right Half-Site RVD Score: 5.322
	Genomic Region: Intron	Closest Gene: PRKG1
Match Type: R10R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GGATC TGTACCTTCCTCATAT                 GAACATAATG                 ATAGTAGTCATGTTCA TTAAG-3'
	3'-CCTAG ACATGGAAGGAGTATA                 CTTGTATTAC                 TATCATCAGTACAAGT AATTC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 010-052784032
Forward Primer: 5'-ACTGAGGCCCAAATCATAGCCAATGATGGA-3' Tm: 61.548
Reverse Primer: 5'-AGGCCTCCACAGCCATGCTT-3' Tm: 59.508
Expected PCR Product Length: 416
Expected CelI Cleavage Product Sizes: 131, 285
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr10:52784140-52784181

Rank: 90	Homology Score: 5	RVD Match Score: 3.012	Left Half-Site RVD Score: 5.648	Right Half-Site RVD Score: 5.899
	Genomic Region: Intergenic	Closest Gene: SORCS1
Match Type: R15R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TAATA TGCACCAGACTTCTAG              TATTAAATATTGGGT               AAATGAGTAATGTGTA CCCAT-3'
	3'-ATTAT ACGTGGTCTGAAGATC              ATAATTTATAACCCA               TTTACTCATTACACAT GGGTA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 010-107873570
Forward Primer: 5'-GACACCTTCTGCTAAGCACACATC-3' Tm: 59.038
Reverse Primer: 5'-GAGCTCATTCAAAGCCCCAGTAG-3' Tm: 59.148
Expected PCR Product Length: 358
Expected CelI Cleavage Product Sizes: 183, 175
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr10:107873728-107873774

Rank: 91	Homology Score: 2	RVD Match Score: 3.012	Left Half-Site RVD Score: 6.493	Right Half-Site RVD Score: 5.096
	Genomic Region: Intron	Closest Gene: GLS
Match Type: R11R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CTCTA TGCATTTACCTCCTTT                CCCTAAAATGT                 ATCGGATTGAGGTGTA GGAGT-3'
	3'-GAGAT ACGTAAATGGAGGAAA                GGGATTTTACA                 TAGCCTAACTCCACAT CCTCA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 002-191816679
Forward Primer: 5'-CCAACAGTTTGGGAGGCCAAG-3' Tm: 59.378
Reverse Primer: 5'-CTGCCCAGAGTCCAGAACAGT-3' Tm: 59.378
Expected PCR Product Length: 275
Expected CelI Cleavage Product Sizes: 130, 145
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr2:191816786-191816828

Rank: 92	Homology Score: 10	RVD Match Score: 3.013	Left Half-Site RVD Score: 4.802	Right Half-Site RVD Score: 6.843
	Genomic Region: Intergenic	Closest Gene: DKFZp686K1684
Match Type: R11R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GAACT TTCACCTGACTCCTTA                TGCTTAGCTCA                 AACGGGGGCAGGTTCA AGCAG-3'
	3'-CTTGA AAGTGGACTGAGGAAT                ACGAATCGAGT                 TTGCCCCCGTCCAAGT TCGTC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 011-031965225
Forward Primer: 5'-CCAGCCTGAGACAACCATCTTTAC-3' Tm: 59.038
Reverse Primer: 5'-GAAGGGCCATAACTTGGGCATTAG-3' Tm: 59.038
Expected PCR Product Length: 355
Expected CelI Cleavage Product Sizes: 192, 163
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr11:31965394-31965436

Rank: 93	Homology Score: 8	RVD Match Score: 3.014	Left Half-Site RVD Score: 5.923	Right Half-Site RVD Score: 5.642
	Genomic Region: Intron	Closest Gene: VASH2
Match Type: R12R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CATTT TTCACCAGACTCCCGG                CTCTTCAGCCTG                AGAGGAATATGGTGCA GAGAA-3'
	3'-GTAAA AAGTGGTCTGAGGGCC                GAGAAGTCGGAC                TCTCCTTATACCACGT CTCTT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 001-213140398
Forward Primer: 5'-ATTCTAAGAGTGGCGTGTGGAGG-3' Tm: 59.148
Reverse Primer: 5'-GTTGTGGGTGATTCCAATGGCATG-3' Tm: 59.038
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 134, 136
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr1:213140508-213140551

Rank: 94	Homology Score: 10	RVD Match Score: 3.016	Left Half-Site RVD Score: 5.293	Right Half-Site RVD Score: 6.305
	Genomic Region: Intron	Closest Gene: C1orf198
Match Type: R20R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GGGCT TGCCCTTGACACCTGT            CTCAGGCCCAGTTTGGCTAC            ATGTGAGACTGGTGCA GAGTG-3'
	3'-CCCGA ACGGGAACTGTGGACA            GAGTCCGGGTCAAACCGATG            TACACTCTGACCACGT CTCAC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 001-230976433
Forward Primer: 5'-GCGCCAAGGTCTTCTTGAGC-3' Tm: 59.508
Reverse Primer: 5'-GGAGAGTAGGAGACAGCACATG-3' Tm: 59.258
Expected PCR Product Length: 312
Expected CelI Cleavage Product Sizes: 136, 176
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr1:230976541-230976592

Rank: 95	Homology Score: 5	RVD Match Score: 3.019	Left Half-Site RVD Score: 5.747	Right Half-Site RVD Score: 5.848
	Genomic Region: Intron	Closest Gene: SUMF1
Match Type: R19R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GACTT TACTCCTCACTCCTCG            TTTTGTTCACCCCAGAAGC             ACAGCAGTCTGGAGTA AAGTC-3'
	3'-CTGAA ATGAGGAGTGAGGAGC            AAAACAAGTGGGGTCTTCG             TGTCGTCAGACCTCAT TTCAG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 003-004440767
Forward Primer: 5'-TATGAGAACGAGAGGGGGCG-3' Tm: 59.508
Reverse Primer: 5'-CAAGCCCTTCCCCACTGTAG-3' Tm: 59.508
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 136, 134
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr3:4440876-4440926

Rank: 96	Homology Score: 2	RVD Match Score: 3.02	Left Half-Site RVD Score: 5.815	Right Half-Site RVD Score: 5.788
	Genomic Region: Intron	Closest Gene: PTPRD
Match Type: R25R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-AGTCA TACACTTAATCCCTGT         GCCATAATTCAATAAATTGTGGAGG          ATGGGAAGCAGGTGGA GAAAT-3'
	3'-TCAGT ATGTGAATTAGGGACA         CGGTATTAAGTTATTTAACACCTCC          TACCCTTCGTCCACCT CTTTA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 009-009921803
Forward Primer: 5'-CAGCTGATATTATGTGTCCCACACTGTTC-3' Tm: 60.178
Reverse Primer: 5'-GAGTTGCTCACAGCTTCTCTGAGT-3' Tm: 59.038
Expected PCR Product Length: 338
Expected CelI Cleavage Product Sizes: 207, 131
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr9:9921980-9922036

Rank: 97	Homology Score: 8	RVD Match Score: 3.02	Left Half-Site RVD Score: 5.428	Right Half-Site RVD Score: 6.19
	Genomic Region: Intron	Closest Gene: MGMT
Match Type: R30R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-ACACA TCCACGTAACTCTTGT       CCTTAGCAGTAGTGAGGGGCTTCTGCAGGC       AGAGGAGTCGGCTCCA CGGTC-3'
	3'-TGTGT AGGTGCATTGAGAACA       GGAATCGTCATCACTCCCCGAAGACGTCCG       TCTCCTCAGCCGAGGT GCCAG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 010-131523914
Forward Primer: 5'-CTCGGAGGTCTGTCCATGCT-3' Tm: 59.508
Reverse Primer: 5'-GAGCTAGAAGGGGCTGGTTG-3' Tm: 59.508
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 135, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr10:131524016-131524077

Rank: 98	Homology Score: 10	RVD Match Score: 3.02	Left Half-Site RVD Score: 6.224	Right Half-Site RVD Score: 5.399
	Genomic Region: Intron	Closest Gene: TMEM72-AS1
Match Type: R29R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GGGGG TGCCCATGACTCCCCC       TTAGGTTTAACAATGCACTTGAATGACTC        ACAGGACTCAGGAGGA CACTC-3'
	3'-CCCCC ACGGGTACTGAGGGGG       AATCCAAATTGTTACGTGAACTTACTGAG        TGTCCTGAGTCCTCCT GTGAG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 010-045378787
Forward Primer: 5'-GGTGAGGCTCCATCCCACAA-3' Tm: 59.508
Reverse Primer: 5'-ACCCAGAGAGGGTGTGAGAG-3' Tm: 59.508
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 133, 137
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr10:45378888-45378948

Rank: 99	Homology Score: 5	RVD Match Score: 3.022	Left Half-Site RVD Score: 6.496	Right Half-Site RVD Score: 5.163
	Genomic Region: Intron	Closest Gene: CABIN1
Match Type: R25R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TGTGT TGCTTCTCACTCCTAG         GTGCTTCATTTTCTAGTTATTCTTG          ATAGGAGTGACTTGCA GTCTA-3'
	3'-ACACA ACGAAGAGTGAGGATC         CACGAAGTAAAAGATCAATAAGAAC          TATCCTCACTGAACGT CAGAT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 022-024444126
Forward Primer: 5'-TGCTCTCTCCTCCCAAGCAC-3' Tm: 59.508
Reverse Primer: 5'-GGTCTCACCAGTGAACACACAG-3' Tm: 59.258
Expected PCR Product Length: 272
Expected CelI Cleavage Product Sizes: 130, 142
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr22:24444226-24444282

Rank: 100	Homology Score: 10	RVD Match Score: 3.022	Left Half-Site RVD Score: 7.259	Right Half-Site RVD Score: 4.527
	Genomic Region: Intergenic	Closest Gene: GRXCR1
Match Type: R25R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TTGTA TCCACCTGGCAGCTGG         TATCATGTAGCACCGCTTATCTTGC          AAAAGAGTTAGGTGCA TGGGT-3'
	3'-AACAT AGGTGGACCGTCGACC         ATAGTACATCGTGGCGAATAGAACG          TTTTCTCAATCCACGT ACCCA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 004-043460367
Forward Primer: 5'-GCTAAGAGCCTCTTGGGCTTTTG-3' Tm: 59.148
Reverse Primer: 5'-AGCAGAGGCAGAAAGGACCAG-3' Tm: 59.378
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 136, 134
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr4:43460473-43460529

Rank: 101	Homology Score: 5	RVD Match Score: 3.023	Left Half-Site RVD Score: 7.565	Right Half-Site RVD Score: 4.291
	Genomic Region: Intergenic	Closest Gene: GPR20
Match Type: R17R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-AGAAG TGTGCCGGACACCTGA             GGAAGGTCCTTCTGCAG              ATGGGAGGCAGGTGTA TCCGC-3'
	3'-TCTTC ACACGGCCTGTGGACT             CCTTCCAGGAAGACGTC              TACCCTCCGTCCACAT AGGCG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 008-142398434
Forward Primer: 5'-GCCTGAGATAGGGACATTGGTG-3' Tm: 59.258
Reverse Primer: 5'-CCCATTCTGAGCGAGCTTGGA-3' Tm: 59.378
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 135, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr8:142398543-142398591

Rank: 102	Homology Score: 10	RVD Match Score: 3.023	Left Half-Site RVD Score: 5.831	Right Half-Site RVD Score: 5.797
	Genomic Region: Intron	Closest Gene: DENND4C
Match Type: R25R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GAGTC TGCAGATGACTTCTGT         TTATTTCCTATAGTACAAAATTAGT          ATGGGAGTGCTGTGCA CAGTG-3'
	3'-CTCAG ACGTCTACTGAAGACA         AATAAAGGATATCATGTTTTAATCA          TACCCTCACGACACGT GTCAC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 009-019336805
Forward Primer: 5'-GTCCATTGATAATCACTCTAGCACAGGTAC-3' Tm: 60.058
Reverse Primer: 5'-GTGTGACTTCCTTCCTTCCCTC-3' Tm: 59.258
Expected PCR Product Length: 422
Expected CelI Cleavage Product Sizes: 199, 223
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr9:19336974-19337030

Rank: 103	Homology Score: 10	RVD Match Score: 3.026	Left Half-Site RVD Score: 5.505	Right Half-Site RVD Score: 6.152
	Genomic Region: Intergenic	Closest Gene: COG2
Match Type: R28R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TTATT TGAACATGACTTCTGT        GCATTCCATTGCACCATGTGGTGGGGAG        AAAGGTGTCTGGTCTA TTTTT-3'
	3'-AATAA ACTTGTACTGAAGACA        CGTAAGGTAACGTGGTACACCACCCCTC        TTTCCACAGACCAGAT AAAAA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 001-230749531
Forward Primer: 5'-CAAAGATGCAAAGGCTGATTTAGAGCACC-3' Tm: 60.178
Reverse Primer: 5'-CCCCAAATTTAGCAATACTGTCTGCTGGA-3' Tm: 60.178
Expected PCR Product Length: 441
Expected CelI Cleavage Product Sizes: 131, 310
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr1:230749630-230749689

Rank: 104	Homology Score: 5	RVD Match Score: 3.027	Left Half-Site RVD Score: 8.515	Right Half-Site RVD Score: 5.743
	Genomic Region: Intron	Closest Gene: OR8U8
Match Type: L17R
	           Left Nuclease
	         TCACCTTGCCCCACAGGGCAGT
	5'-ATGTC TCGCCTCCCGCCACAGGGCGGT       TTTTCTCCTATCTCAGA              ATAGAAGAAAGGTGCA ATCAG-3'
	3'-TACAG AGCGGAGGGCGGTGTCCCGCCA       AAAAGAGGATAGAGTCT              TATCTTCTTTCCACGT TAGTC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 011-056386094
Forward Primer: 5'-GGGAGGTACTATGCCTGGATG-3' Tm: 59.378
Reverse Primer: 5'-GCTCGGGAGGATTAGTAAAAGAGG-3' Tm: 59.038
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 136, 134
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr11:56386201-56386255

Rank: 105	Homology Score: 2	RVD Match Score: 3.027	Left Half-Site RVD Score: 5.406	Right Half-Site RVD Score: 6.273
	Genomic Region: Intron	Closest Gene: RBKS
Match Type: R17R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TGCAC TGCACCCACCTCCTCA             CAGAGCTGTGCGAAGAG              ACGTGAGTTAAGGGCA TGTAC-3'
	3'-ACGTG ACGTGGGTGGAGGAGT             GTCTCGACACGCTTCTC              TGCACTCAATTCCCGT ACATG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 002-028093265
Forward Primer: 5'-GCTGCCAGTGGTGACTGTTTTTC-3' Tm: 59.148
Reverse Primer: 5'-GTGAGTCTACACACCATGTCATCC-3' Tm: 59.038
Expected PCR Product Length: 292
Expected CelI Cleavage Product Sizes: 154, 138
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr2:28093393-28093441

Rank: 106	Homology Score: 2	RVD Match Score: 3.028	Left Half-Site RVD Score: 5.495	Right Half-Site RVD Score: 6.182
	Genomic Region: Intron	Closest Gene: LOC339975
Match Type: R27R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-ATTTA TACACCTGAGCCCTAA        AAGTCTGGGGTCATTCAGTGTTTCTGA         AGGGCAGGCAGGTGGA TATCA-3'
	3'-TAAAT ATGTGGACTCGGGATT        TTCAGACCCCAGTAAGTCACAAAGACT         TCCCGTCCGTCCACCT ATAGT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 004-188278629
Forward Primer: 5'-CCTTTCCCTTACCCTGTTTGTTCC-3' Tm: 59.038
Reverse Primer: 5'-GTGTCCTAGGAACACTGCTCTG-3' Tm: 59.258
Expected PCR Product Length: 404
Expected CelI Cleavage Product Sizes: 260, 144
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr4:188278858-188278916

Rank: 107	Homology Score: 10	RVD Match Score: 3.029	Left Half-Site RVD Score: 5.891	Right Half-Site RVD Score: 5.788
	Genomic Region: Intron	Closest Gene: ZZEF1
Match Type: R29R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-ACTAC TGCCACAGACTCCTAA       CTGCTTTTCTTATTTCCACCTCTTTTCAC        ACAGCAGCCAGGGGCA CCTCT-3'
	3'-TGATG ACGGTGTCTGAGGATT       GACGAAAAGAATAAAGGTGGAGAAAAGTG        TGTCGTCGGTCCCCGT GGAGA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 017-003965044
Forward Primer: 5'-CGTAAGTCCTGACAACTCTACCTC-3' Tm: 59.038
Reverse Primer: 5'-GAAAGGCAGGACCCTTATCACTGT-3' Tm: 59.038
Expected PCR Product Length: 290
Expected CelI Cleavage Product Sizes: 143, 147
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr17:3965155-3965215

Rank: 108	Homology Score: 5	RVD Match Score: 3.031	Left Half-Site RVD Score: 6.491	Right Half-Site RVD Score: 5.231
	Genomic Region: Exon	Closest Gene: PHF20
Match Type: R18R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CTGAA TGCACGTGACACCGAC             TGACTTCAGGGATCTGGG             CCAGGAGTGTGGTGGA CATTG-3'
	3'-GACTT ACGTGCACTGTGGCTG             ACTGAAGTCCCTAGACCC             GGTCCTCACACCACCT GTAAC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 020-034535532
Forward Primer: 5'-CTCTGCTGCTCAACATGAAACTGG-3' Tm: 59.038
Reverse Primer: 5'-TTCAACGATGTGCACAGAAGAGGG-3' Tm: 59.038
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 136, 134
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr20:34535641-34535690

Rank: 109	Homology Score: 10	RVD Match Score: 3.031	Left Half-Site RVD Score: 7.213	Right Half-Site RVD Score: 4.624
	Genomic Region: Intron	Closest Gene: WNT10A
Match Type: R26R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GTGTC TGCCCCAGACAGCTGG         GGCCAGAACCGAAACTGGGGCCCTGA         ACAGGAGCTGGGTGCA GACCC-3'
	3'-CACAG ACGGGGTCTGTCGACC         CCGGTCTTGGCTTTGACCCCGGGACT         TGTCCTCGACCCACGT CTGGG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 002-219751327
Forward Primer: 5'-TGTGTCTTAACGAGCCGCCC-3' Tm: 59.508
Reverse Primer: 5'-CTGACAGCTGTCATAGCCACG-3' Tm: 59.378
Expected PCR Product Length: 284
Expected CelI Cleavage Product Sizes: 154, 130
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr2:219751450-219751507

Rank: 110	Homology Score: 5	RVD Match Score: 3.032	Left Half-Site RVD Score: 5.531	Right Half-Site RVD Score: 6.179
	Genomic Region: Intron	Closest Gene: LONRF1
Match Type: R26R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GTCTG TACAACTGAGTCCAGT         TTCTCTTCTCTTTATTCACCCAGAGA         AGCAGAGTCATGTGTA TCAGA-3'
	3'-CAGAC ATGTTGACTCAGGTCA         AAGAGAAGAGAAATAAGTGGGTCTCT         TCGTCTCAGTACACAT AGTCT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 008-012610456
Forward Primer: 5'-GCCTCTCTACCAGTTAACAGTGTG-3' Tm: 59.038
Reverse Primer: 5'-CGAAGCACAAACCCCGGAGA-3' Tm: 59.508
Expected PCR Product Length: 280
Expected CelI Cleavage Product Sizes: 134, 146
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr8:12610559-12610616

Rank: 111	Homology Score: 5	RVD Match Score: 3.033	Left Half-Site RVD Score: 7.682	Right Half-Site RVD Score: 4.27
	Genomic Region: Intron	Closest Gene: GRM8
Match Type: R25R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GCCAC TGCACCTGGTCCAGGT         TTGTTATACAGGTAAAGGGTATGTC          ACAGGGGTTTGGTGTA CATAT-3'
	3'-CGGTG ACGTGGACCAGGTCCA         AACAATATGTCCATTTCCCATACAG          TGTCCCCAAACCACAT GTATA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 007-126235510
Forward Primer: 5'-GGGGTTTTGTCATGTTGCCCG-3' Tm: 59.378
Reverse Primer: 5'-GCGACCAGCCGGACTATAAATC-3' Tm: 59.258
Expected PCR Product Length: 410
Expected CelI Cleavage Product Sizes: 130, 280
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr7:126235610-126235666

Rank: 112	Homology Score: 13	RVD Match Score: 3.034	Left Half-Site RVD Score: 6.826	Right Half-Site RVD Score: 4.963
	Genomic Region: Intron	Closest Gene: SORBS2
Match Type: R12R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-AATGT TTTTCCTGACTTCTGT                GATGTATAGGGC                ACTGTAGTCAGGTGAA ATAAC-3'
	3'-TTACA AAAAGGACTGAAGACA                CTACATATCCCG                TGACATCAGTCCACTT TATTG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 004-186747672
Forward Primer: 5'-CTGTGAGCTACTGAGGATATGCG-3' Tm: 59.148
Reverse Primer: 5'-GGGCAGATGCTTAAATCACTCCCA-3' Tm: 59.038
Expected PCR Product Length: 374
Expected CelI Cleavage Product Sizes: 218, 156
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr4:186747866-186747909

Rank: 113	Homology Score: 10	RVD Match Score: 3.035	Left Half-Site RVD Score: 5.323	Right Half-Site RVD Score: 6.422
	Genomic Region: Intron	Closest Gene: FAM134B
Match Type: R20R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-AAGAT TGCCCCTGACTCAGGT            CCTTGGAAACCCAGAGTTGC            TCAGGATACAGTTGTA AGCAG-3'
	3'-TTCTA ACGGGGACTGAGTCCA            GGAACCTTTGGGTCTCAACG            AGTCCTATGTCAACAT TCGTC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 005-016519335
Forward Primer: 5'-CCTAGATCAGCGGCTTTCAAAGCA-3' Tm: 59.038
Reverse Primer: 5'-GGGCTGAAAGGAGCTTTGAGG-3' Tm: 59.378
Expected PCR Product Length: 374
Expected CelI Cleavage Product Sizes: 189, 185
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr5:16519496-16519547

Rank: 114	Homology Score: 10	RVD Match Score: 3.035	Left Half-Site RVD Score: 4.527	Right Half-Site RVD Score: 7.366
	Genomic Region: Intergenic	Closest Gene: EDIL3
Match Type: R12R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GTCCA TGTACCTAACTCCTTT                GTCTTCAGCATG                AGAGAACACAGGGGCA TACTG-3'
	3'-CAGGT ACATGGATTGAGGAAA                CAGAAGTCGTAC                TCTCTTGTGTCCCCGT ATGAC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 005-083704636
Forward Primer: 5'-GGAACGGATTAAAGGGAAGCCAG-3' Tm: 59.148
Reverse Primer: 5'-GGTCTGTTTCTGTAACATCAGAGGCAAAG-3' Tm: 60.178
Expected PCR Product Length: 361
Expected CelI Cleavage Product Sizes: 133, 228
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr5:83704745-83704788

Rank: 115	Homology Score: 10	RVD Match Score: 3.035	Left Half-Site RVD Score: 4.592	Right Half-Site RVD Score: 7.284
	Genomic Region: Intron	Closest Gene: BDNF-AS
Match Type: R12R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TCTGA TACCTCTGACTCCTGT                TTACCTAGAGGA                AGAAGAATCTGGAGCA GCAGT-3'
	3'-AGACT ATGGAGACTGAGGACA                AATGGATCTCCT                TCTTCTTAGACCTCGT CGTCA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 011-027668392
Forward Primer: 5'-GGTGGGTCCATTCTGACTCTG-3' Tm: 59.378
Reverse Primer: 5'-TCATGATCCACCCACCTCGG-3' Tm: 59.508
Expected PCR Product Length: 362
Expected CelI Cleavage Product Sizes: 219, 143
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr11:27668587-27668630

Rank: 116	Homology Score: 2	RVD Match Score: 3.035	Left Half-Site RVD Score: 5.551	Right Half-Site RVD Score: 6.18
	Genomic Region: Intergenic	Closest Gene: CHD1L
Match Type: R18R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-ATATC TACATATAACACCTGT             AATCCCAGTACTTTGGGA             GAATGAGGCAGGTGGA TCACC-3'
	3'-TATAG ATGTATATTGTGGACA             TTAGGGTCATGAAACCCT             CTTACTCCGTCCACCT AGTGG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 001-146791343
Forward Primer: 5'-GACTGCCTGCCTTAAAAAGCTTGG-3' Tm: 59.038
Reverse Primer: 5'-ATCTCTGGGATTGGGCCTGG-3' Tm: 59.508
Expected PCR Product Length: 389
Expected CelI Cleavage Product Sizes: 239, 150
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr1:146791555-146791604

Rank: 117	Homology Score: 17	RVD Match Score: 3.036	Left Half-Site RVD Score: 4.181	Right Half-Site RVD Score: 7.83
	Genomic Region: Intron	Closest Gene: SDK1
Match Type: R13R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TGTTT TGAACCTGACTCCTTT               CACATGGCATAGC                ACATGTGACACATGCA TATCA-3'
	3'-ACAAA ACTTGGACTGAGGAAA               GTGTACCGTATCG                TGTACACTGTGTACGT ATAGT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 007-003795640
Forward Primer: 5'-CCTGACTCCTTTCACATGGCATAG-3' Tm: 59.038
Reverse Primer: 5'-TGTGTCACACGTGCTATGCCATG-3' Tm: 59.148
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 135, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr7:3795751-3795795

Rank: 118	Homology Score: 17	RVD Match Score: 3.036	Left Half-Site RVD Score: 4.181	Right Half-Site RVD Score: 7.83
	Genomic Region: Intron	Closest Gene: SDK1
Match Type: R13R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TGTTT TGAACCTGACTCCTTT               CACATGGCATAGC                ACATGTGACACATGCA TATCA-3'
	3'-ACAAA ACTTGGACTGAGGAAA               GTGTACCGTATCG                TGTACACTGTGTACGT ATAGT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 007-003795876
Forward Primer: 5'-GAGTCCTTTCACATGGCATAGCAC-3' Tm: 59.038
Reverse Primer: 5'-ACATGTCTCACACGCACAATGCC-3' Tm: 59.148
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 135, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr7:3795987-3796031

Rank: 119	Homology Score: 2	RVD Match Score: 3.036	Left Half-Site RVD Score: 5.242	Right Half-Site RVD Score: 6.521
	Genomic Region: Intergenic	Closest Gene: CRNDE
Match Type: R21R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CTACT TACACATAACTACTTT           GGTTAAAAAAGTAGGCAGGGG            TGGGGAGCCGGGTGCA CAGTG-3'
	3'-GATGA ATGTGTATTGATGAAA           CCAATTTTTTCATCCGTCCCC            ACCCCTCGGCCCACGT GTCAC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 016-054900620
Forward Primer: 5'-GGATCTAGCCCTTGAGTGTGG-3' Tm: 59.378
Reverse Primer: 5'-GGGGTTTCACCATGTTATGTTGGC-3' Tm: 59.038
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 138, 132
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr16:54900730-54900782

Rank: 120	Homology Score: 5	RVD Match Score: 3.036	Left Half-Site RVD Score: 5.669	Right Half-Site RVD Score: 6.063
	Genomic Region: Intergenic	Closest Gene: FLRT2
Match Type: R26R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-AGTGT TGTCCCTGACTCCGAT         TCTCATTGTCCTATTCTCAATCGGGA         AATGGAGGCTGGTGGA ACCAA-3'
	3'-TCACA ACAGGGACTGAGGCTA         AGAGTAACAGGATAAGAGTTAGCCCT         TTACCTCCGACCACCT TGGTT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 014-086334283
Forward Primer: 5'-CACACACACACACACCCTTTGATC-3' Tm: 59.038
Reverse Primer: 5'-CAGCAACGCTGACCTAGAAACTC-3' Tm: 59.148
Expected PCR Product Length: 419
Expected CelI Cleavage Product Sizes: 255, 164
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr14:86334507-86334564

Rank: 121	Homology Score: 2	RVD Match Score: 3.038	Left Half-Site RVD Score: 5.403	Right Half-Site RVD Score: 6.364
	Genomic Region: Intergenic	Closest Gene: CIT
Match Type: R10R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TTGGG TAAAAATGACTCCTAT                 CCATATCATA                 ATAGGACCCAGGAGTA TTGCC-3'
	3'-AACCC ATTTTTACTGAGGATA                 GGTATAGTAT                 TATCCTGGGTCCTCAT AACGG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 012-120352694
Forward Primer: 5'-CGGGTCTGTTTAGAGAGAGGG-3' Tm: 59.378
Reverse Primer: 5'-GGCCTCCCAAAATGCTGAGATTAC-3' Tm: 59.038
Expected PCR Product Length: 299
Expected CelI Cleavage Product Sizes: 153, 146
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr12:120352824-120352865

Rank: 122	Homology Score: 10	RVD Match Score: 3.039	Left Half-Site RVD Score: 5.391	Right Half-Site RVD Score: 6.381
	Genomic Region: Intron	Closest Gene: MBP
Match Type: R20R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GGCTC TGCTCCTCTCTCCTGT            CTTGGCTGGCAGGGGCTGCC            CCTGGGGTCGGGTGGA CTCCA-3'
	3'-CCGAG ACGAGGAGAGAGGACA            GAACCGACCGTCCCCGACGG            GGACCCCAGCCCACCT GAGGT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 018-074790610
Forward Primer: 5'-GAGCTGTGGACTGAAGAGCAAC-3' Tm: 59.258
Reverse Primer: 5'-CACAGTCTTGGGAGGGTGCT-3' Tm: 59.508
Expected PCR Product Length: 304
Expected CelI Cleavage Product Sizes: 133, 171
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr18:74790715-74790766

Rank: 123	Homology Score: 10	RVD Match Score: 3.039	Left Half-Site RVD Score: 6.867	Right Half-Site RVD Score: 4.963
	Genomic Region: Intron	Closest Gene: PCDH9
Match Type: R17R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-ATGCT TGCACCACAGTTCTTT             GTGAATGGGGGATGTGG              ACTGGATTCAGGTGAA AGAAA-3'
	3'-TACGA ACGTGGTGTCAAGAAA             CACTTACCCCCTACACC              TGACCTAAGTCCACTT TCTTT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 013-067512369
Forward Primer: 5'-GGCATCAACAGGGCTTCTTAAACG-3' Tm: 59.038
Reverse Primer: 5'-CAATGTCAGTCGTTTCTGCATGAAGATAGG-3' Tm: 60.058
Expected PCR Product Length: 388
Expected CelI Cleavage Product Sizes: 227, 161
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr13:67512570-67512618

Rank: 124	Homology Score: 5	RVD Match Score: 3.041	Left Half-Site RVD Score: 6.486	Right Half-Site RVD Score: 5.309
	Genomic Region: Intron	Closest Gene: GPBP1
Match Type: R22R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-AGTTT TAAAACTGAATTCTGT           ACCATAATGTAGATGAAAAAAG           ACTGGAGTAAGATGTA TTTGC-3'
	3'-TCAAA ATTTTGACTTAAGACA           TGGTATTACATCTACTTTTTTC           TGACCTCATTCTACAT AAACG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 005-056491247
Forward Primer: 5'-GTGTACCACGTCTGGCCTAG-3' Tm: 59.508
Reverse Primer: 5'-GCGCCCAGCCGAAAAACGTA-3' Tm: 59.508
Expected PCR Product Length: 307
Expected CelI Cleavage Product Sizes: 165, 142
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr5:56491383-56491436

Rank: 125	Homology Score: 8	RVD Match Score: 3.043	Left Half-Site RVD Score: 5.703	Right Half-Site RVD Score: 6.082
	Genomic Region: Intergenic	Closest Gene: CA5A
Match Type: R11R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TGTCC TTCAGCAGACTCCTCT                TGATCGATGGA                 ATCGAAGTCAGGCGCA GCAGC-3'
	3'-ACAGG AAGTCGTCTGAGGAGA                ACTAGCTACCT                 TAGCTTCAGTCCGCGT CGTCG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 016-087916217
Forward Primer: 5'-TGGGCCCAACATGTCAGTGC-3' Tm: 59.508
Reverse Primer: 5'-GAGATCACACTTGGTGGCGATG-3' Tm: 59.258
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 135, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr16:87916329-87916371

Rank: 126	Homology Score: 10	RVD Match Score: 3.044	Left Half-Site RVD Score: 6.519	Right Half-Site RVD Score: 5.301
	Genomic Region: Intergenic	Closest Gene: SALL3
Match Type: R16R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CACTC TGCACCTGCAGCCTCC              TCCTGATGGATGACCA              ACAGGAGCAAGGGGCA AGGCA-3'
	3'-GTGAG ACGTGGACGTCGGAGG              AGGACTACCTACTGGT              TGTCCTCGTTCCCCGT TCCGT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 018-076246512
Forward Primer: 5'-AGCCTCCTACTGATGGGTGG-3' Tm: 59.508
Reverse Primer: 5'-GCTGATGAAGAAGAGGATGGGG-3' Tm: 59.258
Expected PCR Product Length: 286
Expected CelI Cleavage Product Sizes: 151, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr18:76246637-76246684

Rank: 127	Homology Score: 5	RVD Match Score: 3.046	Left Half-Site RVD Score: 5.828	Right Half-Site RVD Score: 5.981
	Genomic Region: Intergenic	Closest Gene: SLC35B3
Match Type: R28R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CAGTT TCCACCAAAGCCCTGT        AACAGGCCAAGAGCTGTCTCTCAAAAGG        AGAGTAGTCATCTGCA GAAGA-3'
	3'-GTCAA AGGTGGTTTCGGGACA        TTGTCCGGTTCTCGACAGAGAGTTTTCC        TCTCATCAGTAGACGT CTTCT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 006-008406248
Forward Primer: 5'-GTACACGACCTGCTCTATGGC-3' Tm: 59.378
Reverse Primer: 5'-GTGCTTGAGATGTCAGTGGCAG-3' Tm: 59.258
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 136, 134
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr6:8406352-8406411

Rank: 128	Homology Score: 5	RVD Match Score: 3.046	Left Half-Site RVD Score: 6.002	Right Half-Site RVD Score: 5.808
	Genomic Region: Exon	Closest Gene: KLHDC8A
Match Type: R27R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-ACTGC TCTACCTGCCTCCTGG        AGATGGGGGTGGGGGGAGGTGGGACTC         ACAGGAGGGAGAGGTA GGAAA-3'
	3'-TGACG AGATGGACGGAGGACC        TCTACCCCCACCCCCCTCCACCCTGAG         TGTCCTCCCTCTCCAT CCTTT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 001-205306079
Forward Primer: 5'-GGTTTGGAACCCCCAATCATTCTC-3' Tm: 59.038
Reverse Primer: 5'-GGGGCTCTAAGTTGACCTCTC-3' Tm: 59.378
Expected PCR Product Length: 315
Expected CelI Cleavage Product Sizes: 139, 176
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr1:205306187-205306245

Rank: 129	Homology Score: 5	RVD Match Score: 3.047	Left Half-Site RVD Score: 5.669	Right Half-Site RVD Score: 6.151
	Genomic Region: Intergenic	Closest Gene: GPBP1
Match Type: R18R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CTGGG TGCACTGGCCTCCTAT             AATCCCAGCATTTTGGGA             AGCTGAGGCAGGTGGA TCACT-3'
	3'-GACCC ACGTGACCGGAGGATA             TTAGGGTCGTAAAACCCT             TCGACTCCGTCCACCT AGTGA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 005-056444609
Forward Primer: 5'-GAGCGATAGAGCAAGACTCTGTCA-3' Tm: 59.038
Reverse Primer: 5'-GGGAATACAGGTGCCCATCACT-3' Tm: 59.258
Expected PCR Product Length: 379
Expected CelI Cleavage Product Sizes: 249, 130
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr5:56444831-56444880

Rank: 130	Homology Score: 5	RVD Match Score: 3.047	Left Half-Site RVD Score: 5.346	Right Half-Site RVD Score: 6.497
	Genomic Region: Intergenic	Closest Gene: ATP1A1
Match Type: R19R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-AACTT TCCCCCAGCCTCCTGT            AGCTATTATTCTCCTGAAG             ACAGGAGGATGGAGGA TTGGC-3'
	3'-TTGAA AGGGGGTCGGAGGACA            TCGATAATAAGAGGACTTC             TGTCCTCCTACCTCCT AACCG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 001-116812599
Forward Primer: 5'-GACTGGACTCAGCTGAGTGG-3' Tm: 59.508
Reverse Primer: 5'-CCTGGAGCACTTGGTGGCAT-3' Tm: 59.508
Expected PCR Product Length: 372
Expected CelI Cleavage Product Sizes: 130, 242
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr1:116812702-116812752

Rank: 131	Homology Score: 10	RVD Match Score: 3.048	Left Half-Site RVD Score: 5.877	Right Half-Site RVD Score: 5.948
	Genomic Region: Intergenic	Closest Gene: KCNJ2
Match Type: R21R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TATGC TACATGTGACTCCTAA           TTAAGTGATCTTTCCAGAACA            CCAAGAGTCAAGTGCA GTCGG-3'
	3'-ATACG ATGTACACTGAGGATT           AATTCACTAGAAAGGTCTTGT            GGTTCTCAGTTCACGT CAGCC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Warning! Primer pair was generated using suboptimal parameters. 
Primer Index: 017-068268613
Forward Primer: 5'-AGGAAGGGATTCTCCTAATAAATTGGCAGATG-3' Tm: 59.848
Reverse Primer: 5'-CTATGGTCCATGTCTAACGTTAATACTACATGC-3' Tm: 59.758
Expected PCR Product Length: 391
Expected CelI Cleavage Product Sizes: 130, 261
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr17:68268715-68268767

Rank: 132	Homology Score: 10	RVD Match Score: 3.051	Left Half-Site RVD Score: 4.522	Right Half-Site RVD Score: 7.513
	Genomic Region: Intergenic	Closest Gene: GOT2
Match Type: R11R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CTGCC TGCACCCCACTCCTGC                TTGTCCAAATC                 AAATGAGACAGGCTCA ATTAA-3'
	3'-GACGG ACGTGGGGTGAGGACG                AACAGGTTTAG                 TTTACTCTGTCCGAGT TAATT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 016-059193695
Forward Primer: 5'-GTAGCCTCTCCCAATACTTAACGG-3' Tm: 59.038
Reverse Primer: 5'-CCTGTGTTGTTTCTGTCCAAGGC-3' Tm: 59.148
Expected PCR Product Length: 332
Expected CelI Cleavage Product Sizes: 130, 202
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr16:59193802-59193844

Rank: 133	Homology Score: 10	RVD Match Score: 3.051	Left Half-Site RVD Score: 6.966	Right Half-Site RVD Score: 4.965
	Genomic Region: Intron	Closest Gene: ME1
Match Type: R22R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CTGTT TGCACTTGACATATCT           GAATAAGATTTCTTTAAATAAG           ACAGGAGTGTCGTGCA CTGAT-3'
	3'-GACAA ACGTGAACTGTATAGA           CTTATTCTAAAGAAATTTATTC           TGTCCTCACAGCACGT GACTA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 006-083995632
Forward Primer: 5'-CAGTTGTTCGGATACGGCTTATCTTCATG-3' Tm: 60.178
Reverse Primer: 5'-GGGTAAGAAAACAGAGGCACAGAG-3' Tm: 59.038
Expected PCR Product Length: 307
Expected CelI Cleavage Product Sizes: 171, 136
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr6:83995774-83995827

Rank: 134	Homology Score: 5	RVD Match Score: 3.052	Left Half-Site RVD Score: 5.362	Right Half-Site RVD Score: 6.516
	Genomic Region: Intergenic	Closest Gene: DACH1
Match Type: R28R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CTATG TACACCCTTCTCCTGT        GGCAGTAGAATGAGCTCTCTCCCTCCCC        ACTAGAGTCTGATGTA CTGAA-3'
	3'-GATAC ATGTGGGAAGAGGACA        CCGTCATCTTACTCGAGAGAGGGAGGGG        TGATCTCAGACTACAT GACTT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 013-072629328
Forward Primer: 5'-GCCAAGCACTGTCTGGAATAACTC-3' Tm: 59.038
Reverse Primer: 5'-CTGGGCTTATTGCATTGTGGGG-3' Tm: 59.258
Expected PCR Product Length: 296
Expected CelI Cleavage Product Sizes: 153, 143
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr13:72629449-72629508

Rank: 135	Homology Score: 10	RVD Match Score: 3.052	Left Half-Site RVD Score: 5.745	Right Half-Site RVD Score: 6.115
	Genomic Region: Intron	Closest Gene: KLHL29
Match Type: R14R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TCCTG TGCACATGAGGCCTGT               GGCCATGTCAGGCC               CTGGGAGAGAGGTGCA AGTCT-3'
	3'-AGGAC ACGTGTACTCCGGACA               CCGGTACAGTCCGG               GACCCTCTCTCCACGT TCAGA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 002-023872858
Forward Primer: 5'-GTGGTCTTAGGCACCTCCTG-3' Tm: 59.508
Reverse Primer: 5'-CTCACTGTCCCCTTAACTTTCCTG-3' Tm: 59.038
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 136, 134
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr2:23872969-23873014

Rank: 136	Homology Score: 8	RVD Match Score: 3.053	Left Half-Site RVD Score: 6.224	Right Half-Site RVD Score: 5.642
	Genomic Region: Intron	Closest Gene: TPD52L2
Match Type: R15R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GGAAG TGCACACGGCTCCAGT              CCTATGGGACATTTG               AAGGGAGTGAGATGCA GCCAG-3'
	3'-CCTTC ACGTGTGCCGAGGTCA              GGATACCCTGTAAAC               TTCCCTCACTCTACGT CGGTC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 020-062498891
Forward Primer: 5'-CCTGGGTGACAGAGTAAGACTCG-3' Tm: 61.088
Reverse Primer: 5'-CCAATATGGTGAAACCCCGTCTC-3' Tm: 59.148
Expected PCR Product Length: 307
Expected CelI Cleavage Product Sizes: 130, 177
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr20:62498996-62499042

Rank: 137	Homology Score: 5	RVD Match Score: 3.053	Left Half-Site RVD Score: 6.615	Right Half-Site RVD Score: 5.281
	Genomic Region: Intergenic	Closest Gene: LOC340094
Match Type: R17R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GCAGG TGAACAACACTCCTGG             AGAGGCACAGCAGAGGC              AAAGGTGTGAGGGGCA GGGCT-3'
	3'-CGTCC ACTTGTTGTGAGGACC             TCTCCGTGTCGTCTCCG              TTTCCACACTCCCCGT CCCGA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 005-004975411
Forward Primer: 5'-CTGTAGGAGAAGGCCAGAGAAATC-3' Tm: 59.038
Reverse Primer: 5'-CCAAAGTGCAGGGATTACAGGC-3' Tm: 59.258
Expected PCR Product Length: 296
Expected CelI Cleavage Product Sizes: 165, 131
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr5:4975550-4975598

Rank: 138	Homology Score: 2	RVD Match Score: 3.053	Left Half-Site RVD Score: 6.525	Right Half-Site RVD Score: 5.362
	Genomic Region: Intron	Closest Gene: CDCA8
Match Type: R17R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GTAGT TTCACCTATCTTCAGT             GGCAAAATGATTCTTTA              ATGGGAGGGAGGTGAA TATTC-3'
	3'-CATCA AAGTGGATAGAAGTCA             CCGTTTTACTAAGAAAT              TACCCTCCCTCCACTT ATAAG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 001-038169360
Forward Primer: 5'-CCTGGGCAACAGAGTGAGAC-3' Tm: 59.508
Reverse Primer: 5'-GGAGTGAGACCCTGCCTCAT-3' Tm: 59.508
Expected PCR Product Length: 302
Expected CelI Cleavage Product Sizes: 158, 144
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr1:38169492-38169540

Rank: 139	Homology Score: 2	RVD Match Score: 3.053	Left Half-Site RVD Score: 6.252	Right Half-Site RVD Score: 5.619
	Genomic Region: Intron	Closest Gene: CPQ
Match Type: R17R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GATAC TGCACCAGAATCTAAT             TTAATCACAGTACCCAG              TCAGGAGTGAGTTTTA ATTGT-3'
	3'-CTATG ACGTGGTCTTAGATTA             AATTAGTGTCATGGGTC              AGTCCTCACTCAAAAT TAACA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 008-098131709
Forward Primer: 5'-GCCCCTTCTTTCCTCACAGAAATG-3' Tm: 59.038
Reverse Primer: 5'-AATGGCTAGCTGCCAGGTGAC-3' Tm: 59.378
Expected PCR Product Length: 413
Expected CelI Cleavage Product Sizes: 282, 131
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr8:98131965-98132013

Rank: 140	Homology Score: 13	RVD Match Score: 3.053	Left Half-Site RVD Score: 5.345	Right Half-Site RVD Score: 6.548
	Genomic Region: Intron	Closest Gene: ERGIC1
Match Type: R10R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-AGGTC TGCTCCTGCCTCCAGT                 AGAAGCAGCC                 ACAGGAGTCCTGGCCA CAGCC-3'
	3'-TCCAG ACGAGGACGGAGGTCA                 TCTTCGTCGG                 TGTCCTCAGGACCGGT GTCGG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 005-172280139
Forward Primer: 5'-TGGTTTACCACGTGCTGTTGTTGC-3' Tm: 59.038
Reverse Primer: 5'-AAGATGCCTCTGGGTGGCCT-3' Tm: 59.508
Expected PCR Product Length: 272
Expected CelI Cleavage Product Sizes: 131, 141
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr5:172280247-172280288

Rank: 141	Homology Score: 5	RVD Match Score: 3.053	Left Half-Site RVD Score: 5.784	Right Half-Site RVD Score: 6.082
	Genomic Region: Intergenic	Closest Gene: SLC7A5P2
Match Type: R11R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TGTCC TTCAGCAAACTCCTCT                TGATCGATGGA                 ATCGAAGTCAGGAGCA TCACC-3'
	3'-ACAGG AAGTCGTTTGAGGAGA                ACTAGCTACCT                 TAGCTTCAGTCCTCGT AGTGG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 016-021539209
Forward Primer: 5'-CAGCATGTCAGTGCGACCCA-3' Tm: 59.508
Reverse Primer: 5'-AGGAGATCACACTTGGTGGTGATG-3' Tm: 59.038
Expected PCR Product Length: 268
Expected CelI Cleavage Product Sizes: 130, 138
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr16:21539316-21539358

Rank: 142	Homology Score: 2	RVD Match Score: 3.054	Left Half-Site RVD Score: 6.796	Right Half-Site RVD Score: 7.288
	Genomic Region: Intron	Closest Gene: CSF2RB
Match Type: L30R
	           Left Nuclease
	         TCACCTTGCCCCACAGGGCAGT
	5'-CTCAT TCACCTTGCCCCATAATTCACT GAGAAGCCGCATCTCAGCCTGGAGGTAGGG       AAGGGGGTCAGGACCA ATCCC-3'
	3'-GAGTA AGTGGAACGGGGTATTAAGTGA CTCTTCGGCGTAGAGTCGGACCTCCATCCC       TTCCCCCAGTCCTGGT TAGGG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 022-037324670
Forward Primer: 5'-ACCCAGAGTTCCCAGGAGATAG-3' Tm: 59.258
Reverse Primer: 5'-CTCAGTGGAATTCCCTCCACC-3' Tm: 59.378
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 136, 134
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr22:37324770-37324837

Rank: 143	Homology Score: 8	RVD Match Score: 3.055	Left Half-Site RVD Score: 5.642	Right Half-Site RVD Score: 6.245
	Genomic Region: Intergenic	Closest Gene: SH2D6
Match Type: R27R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TTTTC TGCACCCCACTCTTTT        CTTTTTTCTAAATTTTATCTTTTTAAA         AGACTAGTCAAGTGCA GTAAT-3'
	3'-AAAAG ACGTGGGGTGAGAAAA        GAAAAAAGATTTAAAATAGAAAAATTT         TCTGATCAGTTCACGT CATTA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 002-085687121
Forward Primer: 5'-ACTGACATCAGAGGTTTCCCAGAG-3' Tm: 59.038
Reverse Primer: 5'-GAGTGGGATACATTGACTGGTCTG-3' Tm: 59.038
Expected PCR Product Length: 269
Expected CelI Cleavage Product Sizes: 137, 132
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr2:85687227-85687285

Rank: 144	Homology Score: 2	RVD Match Score: 3.056	Left Half-Site RVD Score: 7.352	Right Half-Site RVD Score: 4.684
	Genomic Region: Intron	Closest Gene: DCC
Match Type: R22R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-ACAGA TGCACTATACTAGTGT           AAGATGTTAATAATGGGAGAAA           ATAGGTGTGGGGTGTA TGGGA-3'
	3'-TGTCT ACGTGATATGATCACA           TTCTACAATTATTACCCTCTTT           TATCCACACCCCACAT ACCCT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 018-050077032
Forward Primer: 5'-GCCCTGGAATAGACAATACTGCTC-3' Tm: 59.038
Reverse Primer: 5'-CCCTAACATCTGCACATGTACCTC-3' Tm: 59.038
Expected PCR Product Length: 348
Expected CelI Cleavage Product Sizes: 160, 188
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr18:50077163-50077216

Rank: 145	Homology Score: 2	RVD Match Score: 3.057	Left Half-Site RVD Score: 6.062	Right Half-Site RVD Score: 5.828
	Genomic Region: Intron	Closest Gene: PIEZO2
Match Type: R27R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CCTTC TGCAGATAACTACTCT        CCTTTTGAGAGACAGCTCTTGGCTGAC         ACTGGACTTTGGTGGA AACTG-3'
	3'-GGAAG ACGTCTATTGATGAGA        GGAAAACTCTCTGTCGAGAACCGACTG         TGACCTGAAACCACCT TTGAC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 018-011130975
Forward Primer: 5'-GGTGTCAGTGGCAGATAGGG-3' Tm: 59.508
Reverse Primer: 5'-GCTGTGCATGACACGCTTTATGG-3' Tm: 59.148
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 135, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr18:11131079-11131137

Rank: 146	Homology Score: 2	RVD Match Score: 3.057	Left Half-Site RVD Score: 5.828	Right Half-Site RVD Score: 6.062
	Genomic Region: Intron	Closest Gene: MUT
Match Type: R28R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CAGTT TCCACCAAAGTCCAGT        AACAGGCCAAGAGCTGTCTCTCAAAAGG        AGAGTAGTTATCTGCA GAAGA-3'
	3'-GTCAA AGGTGGTTTCAGGTCA        TTGTCCGGTTCTCGACAGAGAGTTTTCC        TCTCATCAATAGACGT CTTCT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 006-049406871
Forward Primer: 5'-GCTGCTGTGCAAGACCCACT-3' Tm: 59.508
Reverse Primer: 5'-GGTGTCAGTGGCAGATAGCAATG-3' Tm: 59.148
Expected PCR Product Length: 275
Expected CelI Cleavage Product Sizes: 142, 133
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr6:49406981-49407040

Rank: 147	Homology Score: 2	RVD Match Score: 3.057	Left Half-Site RVD Score: 5.828	Right Half-Site RVD Score: 6.062
	Genomic Region: Intron	Closest Gene: DCC
Match Type: R28R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CAGTT TCCACCAAAGTCCAGT        AACAGGCCAAGAGCTGTCTCTTAAACAG        AGAGTAGTTATCTGCA GAAGA-3'
	3'-GTCAA AGGTGGTTTCAGGTCA        TTGTCCGGTTCTCGACAGAGAATTTGTC        TCTCATCAATAGACGT CTTCT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 018-050114372
Forward Primer: 5'-GTGCATGACCCACTTTATGGCTAG-3' Tm: 59.038
Reverse Primer: 5'-CTTGAGGTGTCAGTGGTAGTAGG-3' Tm: 59.148
Expected PCR Product Length: 273
Expected CelI Cleavage Product Sizes: 136, 137
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr18:50114476-50114535

Rank: 148	Homology Score: 2	RVD Match Score: 3.057	Left Half-Site RVD Score: 5.828	Right Half-Site RVD Score: 6.062
	Genomic Region: Intergenic	Closest Gene: PCDH10
Match Type: R28R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CAGTT TCCACCAAAGTCCAGT        AACAGGCCAAGTGTTGTCTCTCAAAAGG        AGAGTAGTTATCTGCA GAATA-3'
	3'-GTCAA AGGTGGTTTCAGGTCA        TTGTCCGGTTCACAACAGAGAGTTTTCC        TCTCATCAATAGACGT CTTAT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 004-133032214
Forward Primer: 5'-TGCACCACCCACTTTATGGCTAG-3' Tm: 59.148
Reverse Primer: 5'-GAGGTGTCAGTGGCAGATGG-3' Tm: 59.508
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 135, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr4:133032317-133032376

Rank: 149	Homology Score: 2	RVD Match Score: 3.057	Left Half-Site RVD Score: 6.062	Right Half-Site RVD Score: 5.828
	Genomic Region: Intergenic	Closest Gene: YTHDF3
Match Type: R28R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TCTTC TGCAGATAACTACTCT        CCTTTTGAGAGACAGCTCTCGGCCTGTT        ACTGGACTTTGGTGGA AACTG-3'
	3'-AGAAG ACGTCTATTGATGAGA        GGAAAACTCTCTGTCGAGAGCCGGACAA        TGACCTGAAACCACCT TTGAC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 008-064233767
Forward Primer: 5'-TGTCAGTGGCAGATAGGGATGCT-3' Tm: 59.148
Reverse Primer: 5'-GCTGTGCATGACCCACTTTATGGC-3' Tm: 60.908
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 135, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr8:64233870-64233929

Rank: 150	Homology Score: 2	RVD Match Score: 3.057	Left Half-Site RVD Score: 6.062	Right Half-Site RVD Score: 5.828
	Genomic Region: Intergenic	Closest Gene: SH3BGRL
Match Type: R28R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TCTCC TGCAGATAACTACTCT        TCCTTTGAGAGACAGCTCTTGGCCTATT        ACTGGACTTTGGTGGA AACTG-3'
	3'-AGAGG ACGTCTATTGATGAGA        AGGAAACTCTCTGTCGAGAACCGGATAA        TGACCTGAAACCACCT TTGAC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 00X-080874147
Forward Primer: 5'-TGTCACTGGCAGATAGGGATGCT-3' Tm: 59.148
Reverse Primer: 5'-GCTGTACACATCCCACTTTATGGC-3' Tm: 59.038
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 135, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chrX:80874250-80874309

Rank: 151	Homology Score: 2	RVD Match Score: 3.057	Left Half-Site RVD Score: 6.091	Right Half-Site RVD Score: 5.805
	Genomic Region: Intron	Closest Gene: DNER
Match Type: R30R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CTTTG TTCATCTGACTCCCAA       ACTGCTGCCTGGAAACAGAAAACAGGAGGC       AGAGGAGGAAGCTGTA CTCGG-3'
	3'-GAAAC AAGTAGACTGAGGGTT       TGACGACGGACCTTTGTCTTTTGTCCTCCG       TCTCCTCCTTCGACAT GAGCC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 002-230410259
Forward Primer: 5'-CAGCCTGGGTGACACAGCAA-3' Tm: 59.508
Reverse Primer: 5'-AACTTTGCTCTTTGTGGAGCCGG-3' Tm: 59.148
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 135, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr2:230410361-230410422

Rank: 152	Homology Score: 8	RVD Match Score: 3.058	Left Half-Site RVD Score: 5.823	Right Half-Site RVD Score: 6.076
	Genomic Region: Intergenic	Closest Gene: IPO5
Match Type: R25R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CAATT TGACCCTCACTCTTGT         AGCAGAAATGGTACAAAAGGCGGCA          AGAGGAGAAAGATGCA GAGCA-3'
	3'-GTTAA ACTGGGAGTGAGAACA         TCGTCTTTACCATGTTTTCCGCCGT          TCTCCTCTTTCTACGT CTCGT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 013-098576588
Forward Primer: 5'-GGTCATTCCTGGCTGTGCAAC-3' Tm: 59.378
Reverse Primer: 5'-CTTTGGAAGGGCATAGTGACCTG-3' Tm: 59.148
Expected PCR Product Length: 437
Expected CelI Cleavage Product Sizes: 231, 206
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr13:98576789-98576845

Rank: 153	Homology Score: 10	RVD Match Score: 3.058	Left Half-Site RVD Score: 7.574	Right Half-Site RVD Score: 4.522
	Genomic Region: Intron	Closest Gene: IQGAP2
Match Type: R29R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TGGTC TGAAAGAGACTCGTGT       GAAGGTCTAGAACAGGAAGGGGGAAGTAA        ACGGGTGTGAGGTGCA GCCAG-3'
	3'-ACCAG ACTTTCTCTGAGCACA       CTTCCAGATCTTGTCCTTCCCCCTTCATT        TGCCCACACTCCACGT CGGTC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 005-075922611
Forward Primer: 5'-AGGGTAGCCCGGGAAGACTTT-3' Tm: 59.378
Reverse Primer: 5'-TAAAGCTTCCCCAAAGGCTTCCC-3' Tm: 59.148
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 135, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr5:75922714-75922774

Rank: 154	Homology Score: 2	RVD Match Score: 3.058	Left Half-Site RVD Score: 6.083	Right Half-Site RVD Score: 5.82
	Genomic Region: Intergenic	Closest Gene: PLS3
Match Type: R17R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GAGCA TGCACCTCCTTCCTAG             CAAGGCACGCCTGATTA              ACAGGGGTTTGGAGGA TAGGT-3'
	3'-CTCGT ACGTGGAGGAAGGATC             GTTCCGTGCGGACTAAT              TGTCCCCAAACCTCCT ATCCA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 00X-115065707
Forward Primer: 5'-GAAGGCAGGAGCTAAGAAAGGG-3' Tm: 59.258
Reverse Primer: 5'-GGACCCCCTTCATGTATCTCTC-3' Tm: 59.258
Expected PCR Product Length: 284
Expected CelI Cleavage Product Sizes: 130, 154
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chrX:115065811-115065859

Rank: 155	Homology Score: 10	RVD Match Score: 3.058	Left Half-Site RVD Score: 4.965	Right Half-Site RVD Score: 7.026
	Genomic Region: Intergenic	Closest Gene: HAS2
Match Type: R18R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CCTCT TGGACCTGACACCTCT             TCCCCAAACCAGAGGAAC             TCTGTAGCCAGGGGCA TCAAC-3'
	3'-GGAGA ACCTGGACTGTGGAGA             AGGGGTTTGGTCTCCTTG             AGACATCGGTCCCCGT AGTTG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 008-122428182
Forward Primer: 5'-ACAAGTTCTGCCAACTACACCAGG-3' Tm: 59.038
Reverse Primer: 5'-AGAGCCATTCCCAAGTGGGGT-3' Tm: 59.378
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 134, 136
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr8:122428289-122428338

Rank: 156	Homology Score: 2	RVD Match Score: 3.059	Left Half-Site RVD Score: 6.062	Right Half-Site RVD Score: 5.847
	Genomic Region: Intron	Closest Gene: ZHX2
Match Type: R17R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TCCTG TCCACCTGACTCTCAG             CCCTCAGCCTTTGCTTT              GTAGGAGTAATGGGCA TTTAC-3'
	3'-AGGAC AGGTGGACTGAGAGTC             GGGAGTCGGAAACGAAA              CATCCTCATTACCCGT AAATG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 008-123811913
Forward Primer: 5'-AGGGCTCAGCCTCTGTGACT-3' Tm: 59.508
Reverse Primer: 5'-TGCAGTGAGCTAAGGTCACACCA-3' Tm: 59.148
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 130, 140
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr8:123812017-123812065

Rank: 157	Homology Score: 10	RVD Match Score: 3.059	Left Half-Site RVD Score: 4.688	Right Half-Site RVD Score: 7.374
	Genomic Region: Intergenic	Closest Gene: SPERT
Match Type: R30R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CACAG TGAACCTGATTCCTAT       AGATTTCTCGGTGGCAGGGCAAGAAGGAAC       AGAGCAGGCACATGCA ATGAA-3'
	3'-GTGTC ACTTGGACTAAGGATA       TCTAAAGAGCCACCGTCCCGTTCTTCCTTG       TCTCGTCCGTGTACGT TACTT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 013-046233753
Forward Primer: 5'-CTCCCTATGTTGTCCAGGCTAGA-3' Tm: 59.148
Reverse Primer: 5'-GCTGGGTTTGGTAGTTCTAACTCC-3' Tm: 59.038
Expected PCR Product Length: 362
Expected CelI Cleavage Product Sizes: 188, 174
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr13:46233908-46233969

Rank: 158	Homology Score: 5	RVD Match Score: 3.06	Left Half-Site RVD Score: 4.58	Right Half-Site RVD Score: 7.512
	Genomic Region: Intergenic	Closest Gene: NUDT7
Match Type: R25R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TTAGG TTCACCTACCTCCTCT         GTGCCCAGTTCACTCCCATGAAGCT          ACACATTTCAGTTGCA GCATC-3'
	3'-AATCC AAGTGGATGGAGGAGA         CACGGGTCAAGTGAGGGTACTTCGA          TGTGTAAAGTCAACGT CGTAG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 016-077615266
Forward Primer: 5'-TCGCTCTCCAGCATCGACTC-3' Tm: 59.508
Reverse Primer: 5'-GGTGGAAAGCCATTCACTCCTAG-3' Tm: 59.148
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 136, 134
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr16:77615372-77615428

Rank: 159	Homology Score: 5	RVD Match Score: 3.06	Left Half-Site RVD Score: 5.768	Right Half-Site RVD Score: 6.153
	Genomic Region: Intron	Closest Gene: TLX1NB
Match Type: R17R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CCAGA TCCACGTGCCTCCTGC             ACAGAAGCACCTCACCT              GGAGGGGTGAGGAGCA GAGAG-3'
	3'-GGTCT AGGTGCACGGAGGACG             TGTCTTCGTGGAGTGGA              CCTCCCCACTCCTCGT CTCTC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 010-102872293
Forward Primer: 5'-GTGAGGAGGCCAAGAAAGCTG-3' Tm: 59.378
Reverse Primer: 5'-CATGTGTGTGAGAACCTGTGTGTG-3' Tm: 59.038
Expected PCR Product Length: 319
Expected CelI Cleavage Product Sizes: 130, 189
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr10:102872397-102872445

Rank: 160	Homology Score: 2	RVD Match Score: 3.061	Left Half-Site RVD Score: 5.399	Right Half-Site RVD Score: 6.556
	Genomic Region: Intergenic	Closest Gene: CPNE8
Match Type: R30R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-AAAAT TCCACCTAACACTTCT       GCTTATATATCTCTTTGACCCAAACTTAGT       CAAGGAGCCATGTGTA TCTTT-3'
	3'-TTTTA AGGTGGATTGTGAAGA       CGAATATATAGAGAAACTGGGTTTGAATCA       GTTCCTCGGTACACAT AGAAA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 012-038979055
Forward Primer: 5'-GCTGGTGAGTGCCCACAAAATTAC-3' Tm: 59.038
Reverse Primer: 5'-CGCGGTCCCCTAAACCTAATTTC-3' Tm: 59.148
Expected PCR Product Length: 302
Expected CelI Cleavage Product Sizes: 163, 139
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr12:38979185-38979246

Rank: 161	Homology Score: 10	RVD Match Score: 3.062	Left Half-Site RVD Score: 6.926	Right Half-Site RVD Score: 5.08
	Genomic Region: Intergenic	Closest Gene: LINC00290
Match Type: R30R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GCTTC TGCTCCGAAGTCCTGC       ATGGACATGCTTGTATATATTTATACCATT       AAAGGAGTCAGTTTCA AAAAT-3'
	3'-CGAAG ACGAGGCTTCAGGACG       TACCTGTACGAACATATATAAATATGGTAA       TTTCCTCAGTCAAAGT TTTTA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 004-182299969
Forward Primer: 5'-GACAAGGAAACTGAGGCACAGAAG-3' Tm: 59.038
Reverse Primer: 5'-ACACTGATTCCTGACCTCAGCG-3' Tm: 59.258
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 138, 132
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr4:182300074-182300135

Rank: 162	Homology Score: 5	RVD Match Score: 3.062	Left Half-Site RVD Score: 6.123	Right Half-Site RVD Score: 5.813
	Genomic Region: Intron	Closest Gene: PIGU
Match Type: R25R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GGGAC TGCACCTCACAGCGAT         CTGGGCCTGGGTGGGTGGCACAGCT          ACAGGAGTGAGCAGGA ATGTA-3'
	3'-CCCTG ACGTGGAGTGTCGCTA         GACCCGGACCCACCCACCGTGTCGA          TGTCCTCACTCGTCCT TACAT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 020-033210588
Forward Primer: 5'-GATGCACAGGCACTAGGCTTTC-3' Tm: 59.258
Reverse Primer: 5'-GCGTTTCTGCCTGTCTTGGTATAG-3' Tm: 59.038
Expected PCR Product Length: 400
Expected CelI Cleavage Product Sizes: 257, 143
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr20:33210815-33210871

Rank: 163	Homology Score: 2	RVD Match Score: 3.062	Left Half-Site RVD Score: 5.371	Right Half-Site RVD Score: 6.595
	Genomic Region: Intron	Closest Gene: LHFPL5
Match Type: R17R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GAATG TCCCCCTCACCCCTCT             ACACTCTCAGAGGCTAT              AGGGGAGAGAGCTGCA ATCCA-3'
	3'-CTTAC AGGGGGAGTGGGGAGA             TGTGAGAGTCTCCGATA              TCCCCTCTCTCGACGT TAGGT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 006-035782662
Forward Primer: 5'-GATGGGGACACCAAGACTAATCAG-3' Tm: 59.038
Reverse Primer: 5'-TCATCTGCTGTTGTTCCCCATCTC-3' Tm: 59.038
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 135, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr6:35782771-35782819

Rank: 164	Homology Score: 2	RVD Match Score: 3.063	Left Half-Site RVD Score: 5.362	Right Half-Site RVD Score: 6.608
	Genomic Region: Intergenic	Closest Gene: SLCO6A1
Match Type: R25R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GCATC TTCCCCTAACTCCTCC         TGGATGAAGCCAGCCTCATCCATGG          CTAGGAGTCTTCTGCA AAGTG-3'
	3'-CGTAG AAGGGGATTGAGGAGG         ACCTACTTCGGTCGGAGTAGGTACC          GATCCTCAGAAGACGT TTCAC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 005-101991696
Forward Primer: 5'-GTAGACTCTCCATCGTCCTCAC-3' Tm: 59.258
Reverse Primer: 5'-GGTGACCAGTAATCTCAGGGTCT-3' Tm: 59.148
Expected PCR Product Length: 425
Expected CelI Cleavage Product Sizes: 135, 290
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr5:101991801-101991857

Rank: 165	Homology Score: 10	RVD Match Score: 3.063	Left Half-Site RVD Score: 4.912	Right Half-Site RVD Score: 7.131
	Genomic Region: Intron	Closest Gene: KRT82
Match Type: R23R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CCCCA TGCACCTCTCTCCTTT          CCCTAGAGCCTGGGCAAGGCCAG           GCTGGATTCAGCTTCA CCATT-3'
	3'-GGGGT ACGTGGAGAGAGGAAA          GGGATCTCGGACCCGTTCCGGTC           CGACCTAAGTCGAAGT GGTAA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 012-052789060
Forward Primer: 5'-ATTCCTCCCACCTCCTGCCA-3' Tm: 59.508
Reverse Primer: 5'-TTCAGGGCCCAGAAGCCACA-3' Tm: 59.508
Expected PCR Product Length: 267
Expected CelI Cleavage Product Sizes: 131, 136
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr12:52789162-52789216

Rank: 166	Homology Score: 5	RVD Match Score: 3.064	Left Half-Site RVD Score: 4.741	Right Half-Site RVD Score: 7.345
	Genomic Region: Intergenic	Closest Gene: DDX3Y
Match Type: R18R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-AATGA TCCACCTGCCTCATAT             TCCCAAAGTGCCAGCACT             ACAGGTGTGAGCCACA ATGCC-3'
	3'-TTACT AGGTGGACGGAGTATA             AGGGTTTCACGGTCGTGA             TGTCCACACTCGGTGT TACGG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 00Y-014998617
Forward Primer: 5'-ACTACAGGGGCATGCCACCA-3' Tm: 59.508
Reverse Primer: 5'-TTGGAGGCGAGAAGGCAGTG-3' Tm: 59.508
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 131, 139
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chrY:14998721-14998770

Rank: 167	Homology Score: 5	RVD Match Score: 3.064	Left Half-Site RVD Score: 6.981	Right Half-Site RVD Score: 5.046
	Genomic Region: Intergenic	Closest Gene: OR11H1
Match Type: R25R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-AGCAG TGCTCTGGAATCCTAT         ATGGGGGACAGACACTCAGAATCCA          ACAGCAGTTTGGTGGA ATCTC-3'
	3'-TCGTC ACGAGACCTTAGGATA         TACCCCCTGTCTGTGAGTCTTAGGT          TGTCGTCAAACCACCT TAGAG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 022-016518848
Forward Primer: 5'-GCAGCAGTGTTCCGGTATCC-3' Tm: 59.508
Reverse Primer: 5'-CGGTCACCTAGGATTCCAGAATAC-3' Tm: 59.038
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 133, 137
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr22:16518951-16519007

Rank: 168	Homology Score: 5	RVD Match Score: 3.064	Left Half-Site RVD Score: 5.046	Right Half-Site RVD Score: 6.981
	Genomic Region: Intergenic	Closest Gene: OR11H12
Match Type: R25R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GAGAT TCCACCAAACTGCTGT         TGGATTCTGAGTGTCTGTCCCCCAT          ATAGGATTCCAGAGCA CTGCT-3'
	3'-CTCTA AGGTGGTTTGACGACA         ACCTAAGACTCACAGACAGGGGGTA          TATCCTAAGGTCTCGT GACGA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 014-019308133
Forward Primer: 5'-CCTAGGATTCCAGAATACTGCTGC-3' Tm: 59.038
Reverse Primer: 5'-AACCTAGCAGCAGTGTTCCGG-3' Tm: 59.378
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 134, 136
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr14:19308237-19308293

Rank: 169	Homology Score: 10	RVD Match Score: 3.065	Left Half-Site RVD Score: 4.904	Right Half-Site RVD Score: 7.153
	Genomic Region: Intergenic	Closest Gene: MIR4291
Match Type: R22R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-AGAGC TGCACTTCACTCCTGA           AGCCAGCGAGACCATGAACTCA           CCAGGAGTAAGGAAGA ACTCC-3'
	3'-TCTCG ACGTGAAGTGAGGACT           TCGGTCGCTCTGGTACTTGAGT           GGTCCTCATTCCTTCT TGAGG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 009-096644130
Forward Primer: 5'-CCCCCTTACATAGTGTGGAAGC-3' Tm: 59.258
Reverse Primer: 5'-TCGGAGTTTCTTCCTTCTGGTGG-3' Tm: 59.148
Expected PCR Product Length: 273
Expected CelI Cleavage Product Sizes: 143, 130
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr9:96644244-96644297

Rank: 170	Homology Score: 8	RVD Match Score: 3.065	Left Half-Site RVD Score: 6.051	Right Half-Site RVD Score: 5.904
	Genomic Region: Intergenic	Closest Gene: CDH18
Match Type: R16R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GCAGT TTCCCCTGCCTCTTGT              ATCACTCCTTGGTCCT              ACAGGATACAAGTGTA GCATA-3'
	3'-CGTCA AAGGGGACGGAGAACA              TAGTGAGGAACCAGGA              TGTCCTATGTTCACAT CGTAT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 005-019467940
Forward Primer: 5'-GAGATCAGGCCACAGTCATATTCC-3' Tm: 59.038
Reverse Primer: 5'-CCTGGCGACAGTTGTCATCATC-3' Tm: 59.258
Expected PCR Product Length: 435
Expected CelI Cleavage Product Sizes: 234, 201
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr5:19468148-19468195

Rank: 171	Homology Score: 2	RVD Match Score: 3.065	Left Half-Site RVD Score: 6.13	Right Half-Site RVD Score: 5.828
	Genomic Region: Exon	Closest Gene: CHST15
Match Type: R18R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GCGCG TCAACCTGACTCCCCG             AGCGCTGGGAGGAAAAGT             TTGGGAGTGACGTGCA GACGG-3'
	3'-CGCGC AGTTGGACTGAGGGGC             TCGCGACCCTCCTTTTCA             AACCCTCACTGCACGT CTGCC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 010-125852973
Forward Primer: 5'-CCTAACTTGTACCTGAGAAGCCTG-3' Tm: 59.038
Reverse Primer: 5'-AGTGTTGACTCGGAGGGGGA-3' Tm: 59.508
Expected PCR Product Length: 302
Expected CelI Cleavage Product Sizes: 133, 169
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr10:125853079-125853128

Rank: 172	Homology Score: 5	RVD Match Score: 3.066	Left Half-Site RVD Score: 6.926	Right Half-Site RVD Score: 5.109
	Genomic Region: Intron	Closest Gene: VWF
Match Type: R26R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TCGGG TACTGCTGACTCCAGG         TTGCTGACATGGGGGTTTCCTTTCAC         ACAGGGGTTAGTGGCA TTCAG-3'
	3'-AGCCC ATGACGACTGAGGTCC         AACGACTGTACCCCCAAAGGAAAGTG         TGTCCCCAATCACCGT AAGTC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 012-006059468
Forward Primer: 5'-GGTAAGAGGAAAGGCGAAGTCATG-3' Tm: 59.038
Reverse Primer: 5'-CAGGGCCTTAAATCCACAGCAAC-3' Tm: 59.148
Expected PCR Product Length: 421
Expected CelI Cleavage Product Sizes: 131, 290
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr12:6059568-6059625

Rank: 173	Homology Score: 2	RVD Match Score: 3.067	Left Half-Site RVD Score: 6.858	Right Half-Site RVD Score: 5.169
	Genomic Region: Intron	Closest Gene: IL15
Match Type: R16R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CTTTT TCCACCTGTTTCCCTT              AAGTTTATTTGAGTCC              TTATGTGTTAGGTGCA TCTCT-3'
	3'-GAAAA AGGTGGACAAAGGGAA              TTCAAATAAACTCAGG              AATACACAATCCACGT AGAGA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Warning! Primer pair was generated using suboptimal parameters. 
Primer Index: 004-142620655
Forward Primer: 5'-GGGAGCTCCATTGTTAGGTGC-3' Tm: 59.378
Reverse Primer: 5'-CAACTAACATGATGAATAGAACAGTACTTCACATCTC-3' Tm: 59.428
Expected PCR Product Length: 365
Expected CelI Cleavage Product Sizes: 209, 156
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr4:142620838-142620885

Rank: 174	Homology Score: 8	RVD Match Score: 3.068	Left Half-Site RVD Score: 5.435	Right Half-Site RVD Score: 6.57
	Genomic Region: Intron	Closest Gene: C1orf127
Match Type: R23R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GCATC TGCAGCTACCACCTGT          CTCCCTGGGCCACGTCCTGTGGT           GCAGGAGCCAGGGCCA CCTGC-3'
	3'-CGTAG ACGTCGATGGTGGACA          GAGGGACCCGGTGCAGGACACCA           CGTCCTCGGTCCCGGT GGACG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 001-011023947
Forward Primer: 5'-TTTAATCCCCCCTCCCCTCC-3' Tm: 59.508
Reverse Primer: 5'-AAAGCGTCCACTGTGGGCCCAT-3' Tm: 61.288
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 137, 133
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr1:11024055-11024109

Rank: 175	Homology Score: 5	RVD Match Score: 3.068	Left Half-Site RVD Score: 6.702	Right Half-Site RVD Score: 5.318
	Genomic Region: Intergenic	Closest Gene: MSH3
Match Type: R19R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CAGGG TCAAATTGACTCCAGT            GGTTTAGGATGCAGCCAAG             AGGGGAATCAGGTGGA ATAGA-3'
	3'-GTCCC AGTTTAACTGAGGTCA            CCAAATCCTACGTCGGTTC             TCCCCTTAGTCCACCT TATCT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 005-080195404
Forward Primer: 5'-TAGTTAAGGCACCCATTGACTGGC-3' Tm: 59.038
Reverse Primer: 5'-ACCCAAGACTTCCCCTCGAG-3' Tm: 59.508
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 135, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr5:80195512-80195562

Rank: 176	Homology Score: 13	RVD Match Score: 3.07	Left Half-Site RVD Score: 5.419	Right Half-Site RVD Score: 6.605
	Genomic Region: Intron	Closest Gene: PBRM1
Match Type: R22R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TCTCC TGCAACTGAAGCCTGT           ATTTAATTTAAGCAGGAATGGC           ACTGGAATCAGGCTCA GCAGT-3'
	3'-AGAGG ACGTTGACTTCGGACA           TAAATTAAATTCGTCCTTACCG           TGACCTTAGTCCGAGT CGTCA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 003-052584959
Forward Primer: 5'-GGCTAGGAATCTTAAGCACCCC-3' Tm: 59.258
Reverse Primer: 5'-GAGGGGATAGCAAGGCTGTG-3' Tm: 59.508
Expected PCR Product Length: 378
Expected CelI Cleavage Product Sizes: 246, 132
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr3:52585176-52585229

Rank: 177	Homology Score: 5	RVD Match Score: 3.071	Left Half-Site RVD Score: 7.637	Right Half-Site RVD Score: 4.559
	Genomic Region: Intron	Closest Gene: HIVEP3
Match Type: R14R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GAGTA TTTCCCTGAGTCTTGT               AAGCTTCTAGTACA               AAAGGAGTGTGGTGTA GCACA-3'
	3'-CTCAT AAAGGGACTCAGAACA               TTCGAAGATCATGT               TTTCCTCACACCACAT CGTGT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 001-042392691
Forward Primer: 5'-TCTGTCTCAGGCGAGGATGTAG-3' Tm: 59.258
Reverse Primer: 5'-AGGTGGCCTGTCTCAGTGCA-3' Tm: 59.508
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 138, 132
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr1:42392804-42392849

Rank: 178	Homology Score: 5	RVD Match Score: 3.071	Left Half-Site RVD Score: 6.031	Right Half-Site RVD Score: 5.969
	Genomic Region: Intergenic	Closest Gene: KLHL14
Match Type: R13R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TGCAG TGCATCTGCCTCCATT               GGAGTGCACAGGC                CAGGGAGTGAGGTGGA GGCGA-3'
	3'-ACGTC ACGTAGACGGAGGTAA               CCTCACGTGTCCG                GTCCCTCACTCCACCT CCGCT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 018-030409997
Forward Primer: 5'-GGTTATGTGGCTGTCTGGTTTACCATTTTC-3' Tm: 60.058
Reverse Primer: 5'-CCCAGTCACTGTGGTTTCAGTTC-3' Tm: 59.148
Expected PCR Product Length: 387
Expected CelI Cleavage Product Sizes: 131, 256
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr18:30410104-30410148

Rank: 179	Homology Score: 5	RVD Match Score: 3.072	Left Half-Site RVD Score: 6.615	Right Half-Site RVD Score: 5.421
	Genomic Region: Intron	Closest Gene: NEURL2
Match Type: R18R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GCCTG TGCAACTGAAGCCTCC             AGCACAGGCCTGGCACAA             AGAGGGTTCTGGTGCA TTGAG-3'
	3'-CGGAC ACGTTGACTTCGGAGG             TCGTGTCCGGACCGTGTT             TCTCCCAAGACCACGT AACTC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 020-044517679
Forward Primer: 5'-GAGAGCTGCCACAAAGCTGC-3' Tm: 59.508
Reverse Primer: 5'-GGCTTCGAGGCATAACAAACCC-3' Tm: 59.258
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 135, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr20:44517787-44517836

Rank: 180	Homology Score: 2	RVD Match Score: 3.072	Left Half-Site RVD Score: 6.056	Right Half-Site RVD Score: 5.952
	Genomic Region: Intron	Closest Gene: PAK1
Match Type: R12R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CCCTA TCTACCCCACTCCTTT                TCCTTTACTCAC                GGAGGATTGAGGTTCA ATCCT-3'
	3'-GGGAT AGATGGGGTGAGGAAA                AGGAAATGAGTG                CCTCCTAACTCCAAGT TAGGA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 011-077118244
Forward Primer: 5'-CCCCCTTCCCCTTTTTGTGTTAC-3' Tm: 59.148
Reverse Primer: 5'-AAAGCCTAGGGTTGCCACTGAC-3' Tm: 59.258
Expected PCR Product Length: 302
Expected CelI Cleavage Product Sizes: 172, 130
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr11:77118392-77118435

Rank: 181	Homology Score: 2	RVD Match Score: 3.072	Left Half-Site RVD Score: 6.217	Right Half-Site RVD Score: 5.797
	Genomic Region: Intron	Closest Gene: LHX6
Match Type: R27R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TTGGG TGCAGCTGCCTCCAAC        ACCAGACTGGAAGCCCCTCCAGGAGGC         AGCGGAGTCTGGTTTA CTCCA-3'
	3'-AACCC ACGTCGACGGAGGTTG        TGGTCTGACCTTCGGGGAGGTCCTCCG         TCGCCTCAGACCAAAT GAGGT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 009-124980262
Forward Primer: 5'-CCTCTGGAGCCTGTGACATTC-3' Tm: 59.378
Reverse Primer: 5'-AGGCTGCTAGGGTTGGGACA-3' Tm: 59.508
Expected PCR Product Length: 320
Expected CelI Cleavage Product Sizes: 188, 132
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr9:124980419-124980477

Rank: 182	Homology Score: 10	RVD Match Score: 3.072	Left Half-Site RVD Score: 5.049	Right Half-Site RVD Score: 7.046
	Genomic Region: Intergenic	Closest Gene: MAN1A2
Match Type: R11R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-ATCTT TGACCCTGCCTCCTGT                CTTTATTTCAA                 AAAGGATGCAATTGCA AAGAT-3'
	3'-TAGAA ACTGGGACGGAGGACA                GAAATAAAGTT                 TTTCCTACGTTAACGT TTCTA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Warning! Primer pair was generated using suboptimal parameters. 
Primer Index: 001-118086389
Forward Primer: 5'-CTGTTCATTTTCTTAAGGTGGAGACTTACCTG-3' Tm: 59.848
Reverse Primer: 5'-GCACTCTGGAAACATTCTCTTTCAATGTGTC-3' Tm: 59.958
Expected PCR Product Length: 448
Expected CelI Cleavage Product Sizes: 270, 178
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr1:118086636-118086678

Rank: 183	Homology Score: 5	RVD Match Score: 3.073	Left Half-Site RVD Score: 6.867	Right Half-Site RVD Score: 5.205
	Genomic Region: Intergenic	Closest Gene: FNDC3A
Match Type: R18R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-ATGAT TGCACGTCACCTCTTT             TTTTTCTGTAGATTGGAA             ATAGGAGTCTTTTGCA TCCTG-3'
	3'-TACTA ACGTGCAGTGGAGAAA             AAAAAGACATCTAACCTT             TATCCTCAGAAAACGT AGGAC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 013-049786521
Forward Primer: 5'-TAAAATGTTGGGCTGGGGAAGGAG-3' Tm: 59.038
Reverse Primer: 5'-GTTTGCCTCCTTGAGCCACC-3' Tm: 59.508
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 134, 136
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr13:49786628-49786677

Rank: 184	Homology Score: 17	RVD Match Score: 3.073	Left Half-Site RVD Score: 4.551	Right Half-Site RVD Score: 7.669
	Genomic Region: Intergenic	Closest Gene: GRIK2
Match Type: R29R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CTTGG TGCACCCGACTGCTGT       GGGGTGTCCCAGCAGGAGGTTGTCAATGT        ACTGGAGCAACATGCA ACCTA-3'
	3'-GAACC ACGTGGGCTGACGACA       CCCCACAGGGTCGTCCTCCAACAGTTACA        TGACCTCGTTGTACGT TGGAT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 006-101676033
Forward Primer: 5'-CCAGGTAACATACTTGCTGTCTGC-3' Tm: 59.038
Reverse Primer: 5'-GTCACTACTCAGTACACTTGGACC-3' Tm: 59.038
Expected PCR Product Length: 275
Expected CelI Cleavage Product Sizes: 131, 144
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr6:101676132-101676192

Rank: 185	Homology Score: 2	RVD Match Score: 3.074	Left Half-Site RVD Score: 5.523	Right Half-Site RVD Score: 6.521
	Genomic Region: Intergenic	Closest Gene: KCNK1
Match Type: R14R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TCAAG TGAACCTCCCTCCTAA               GCCTCCCAAAGTGC               TGGGGTGACAGGTGCA ACCCA-3'
	3'-AGTTC ACTTGGAGGGAGGATT               CGGAGGGTTTCACG               ACCCCACTGTCCACGT TGGGT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Warning! Primer pair was generated using suboptimal parameters. 
Primer Index: 001-233731908
Forward Primer: 5'-GTGCTCCTTAATCAGTTCTGTATTCATGTGTC-3' Tm: 59.848
Reverse Primer: 5'-CAGCTAGGTCACTACCAAACTGAG-3' Tm: 59.038
Expected PCR Product Length: 437
Expected CelI Cleavage Product Sizes: 149, 288
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr1:233732032-233732077

Rank: 186	Homology Score: 2	RVD Match Score: 3.074	Left Half-Site RVD Score: 6.639	Right Half-Site RVD Score: 5.42
	Genomic Region: Intron	Closest Gene: GDAP2
Match Type: R26R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GAAAG TACAGCTGAGACCTGA         AGGTCAAACAGGAGGTAGCCATGGGC         AGAGGAGGGAAGTGTA TTCTG-3'
	3'-CTTTC ATGTCGACTCTGGACT         TCCAGTTTGTCCTCCATCGGTACCCG         TCTCCTCCCTTCACAT AAGAC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 001-118423956
Forward Primer: 5'-CTCATGGATCTTGCTGGCTCATGT-3' Tm: 59.038
Reverse Primer: 5'-GTGACTCCAGCGCCTTTGTG-3' Tm: 59.508
Expected PCR Product Length: 325
Expected CelI Cleavage Product Sizes: 188, 137
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr1:118424113-118424170

Rank: 187	Homology Score: 5	RVD Match Score: 3.075	Left Half-Site RVD Score: 7.635	Right Half-Site RVD Score: 4.589
	Genomic Region: Intergenic	Closest Gene: AHI1
Match Type: R26R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CACCT TGCAGCTGAGCCCAGG         GCAGAGGGGCGGGGGTACAGGCTAAG         AGAGGAGGGAGGTGGA AAGCG-3'
	3'-GTGGA ACGTCGACTCGGGTCC         CGTCTCCCCGCCCCCATGTCCGATTC         TCTCCTCCCTCCACCT TTCGC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 006-135592495
Forward Primer: 5'-GAGGCTGTTTCAAGGTACAGATGC-3' Tm: 59.038
Reverse Primer: 5'-GAGGAGGAATCGTATGGAGGG-3' Tm: 59.378
Expected PCR Product Length: 323
Expected CelI Cleavage Product Sizes: 172, 151
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr6:135592636-135592693

Rank: 188	Homology Score: 2	RVD Match Score: 3.075	Left Half-Site RVD Score: 5.788	Right Half-Site RVD Score: 6.252
	Genomic Region: Intergenic	Closest Gene: ARF6
Match Type: R22R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-AGATG TGCCCCTCACATCTAT           CAGTCAGGAAATTCCAAGGGTC           TCAGAAGTTTTGTGCA AGAAA-3'
	3'-TCTAC ACGGGGAGTGTAGATA           GTCAGTCCTTTAAGGTTCCCAG           AGTCTTCAAAACACGT TCTTT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 014-050350504
Forward Primer: 5'-CCTCCTATCTCGTTATAGCTGGG-3' Tm: 59.148
Reverse Primer: 5'-GAGGCCTACTGGAAAGTCAGGA-3' Tm: 59.258
Expected PCR Product Length: 313
Expected CelI Cleavage Product Sizes: 164, 149
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr14:50350639-50350692

Rank: 189	Homology Score: 5	RVD Match Score: 3.076	Left Half-Site RVD Score: 5.823	Right Half-Site RVD Score: 6.221
	Genomic Region: Intron	Closest Gene: CHSY3
Match Type: R26R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-ATCTC TGCCCCTGAATCTTCT         TCAGCTATTCTTCTGAGAATGAATCA         ACAGTAGTTATTTTCA TAATG-3'
	3'-TAGAG ACGGGGACTTAGAAGA         AGTCGATAAGAAGACTCTTACTTAGT         TGTCATCAATAAAAGT ATTAC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 005-129459902
Forward Primer: 5'-GCAGGACAGTATCTTAAGGGCC-3' Tm: 59.258
Reverse Primer: 5'-CCTGGGCAACAGAGTGAGAC-3' Tm: 59.508
Expected PCR Product Length: 293
Expected CelI Cleavage Product Sizes: 131, 162
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr5:129460002-129460059

Rank: 190	Homology Score: 5	RVD Match Score: 3.077	Left Half-Site RVD Score: 6.563	Right Half-Site RVD Score: 5.508
	Genomic Region: Intron	Closest Gene: DPEP1
Match Type: R16R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GGGGC TGGACCTGTCTTCTCT              ACCTCAGTTTCCAAGA              GTAGGAGACAGGGGTA ACAGC-3'
	3'-CCCCG ACCTGGACAGAAGAGA              TGGAGTCAAAGGTTCT              CATCCTCTGTCCCCAT TGTCG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 016-089681728
Forward Primer: 5'-CAGCTCCCACTGGGTACAGA-3' Tm: 59.508
Reverse Primer: 5'-GGGCTCTGAAGACCTGGGAA-3' Tm: 59.508
Expected PCR Product Length: 288
Expected CelI Cleavage Product Sizes: 158, 130
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr16:89681860-89681907

Rank: 191	Homology Score: 5	RVD Match Score: 3.077	Left Half-Site RVD Score: 6.606	Right Half-Site RVD Score: 5.47
	Genomic Region: Intergenic	Closest Gene: RPS29
Match Type: R26R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-AAGGC TCACCCTGACTTCTGA         CGATGTTTCTGGAATACAGTTAATTG         TCAGAAGTCATGTGTA GTGAT-3'
	3'-TTCCG AGTGGGACTGAAGACT         GCTACAAAGACCTTATGTCAATTAAC         AGTCTTCAGTACACAT CACTA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 014-049869550
Forward Primer: 5'-CCAGCATTTCCTGTAACTGTGCC-3' Tm: 59.148
Reverse Primer: 5'-CCAGTTGCTTCCAGGATGTTCCA-3' Tm: 59.148
Expected PCR Product Length: 377
Expected CelI Cleavage Product Sizes: 217, 160
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr14:49869736-49869793

Rank: 192	Homology Score: 5	RVD Match Score: 3.077	Left Half-Site RVD Score: 5.884	Right Half-Site RVD Score: 6.169
	Genomic Region: Intergenic	Closest Gene: ODZ3
Match Type: R15R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-ATTTT TACACATCATCCCTGT              TTAGCAGGAAAGACC               ATAAGAAACAGGTGCA GGACT-3'
	3'-TAAAA ATGTGTAGTAGGGACA              AATCGTCCTTTCTGG               TATTCTTTGTCCACGT CCTGA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 004-183196263
Forward Primer: 5'-CCACCGCACCCAGCAGTATT-3' Tm: 59.508
Reverse Primer: 5'-CTGAGGGTGAAAGCAAGTACAGTG-3' Tm: 59.038
Expected PCR Product Length: 306
Expected CelI Cleavage Product Sizes: 157, 149
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr4:183196395-183196441

Rank: 193	Homology Score: 5	RVD Match Score: 3.078	Left Half-Site RVD Score: 4.883	Right Half-Site RVD Score: 7.29
	Genomic Region: Intergenic	Closest Gene: KCNU1
Match Type: R18R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CAACC TTCACCCAACTCCTTT             GAGGTATCTCAAAAATCC             TCAGGAATAATATGCA ACTGT-3'
	3'-GTTGG AAGTGGGTTGAGGAAA             CTCCATAGAGTTTTTAGG             AGTCCTTATTATACGT TGACA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 008-036489761
Forward Primer: 5'-GCATCACTGTGGTGAAAACAGAGG-3' Tm: 59.038
Reverse Primer: 5'-CCGTTATGTGATGGTGGAGGATTC-3' Tm: 59.038
Expected PCR Product Length: 419
Expected CelI Cleavage Product Sizes: 241, 178
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr8:36489975-36490024

Rank: 194	Homology Score: 5	RVD Match Score: 3.078	Left Half-Site RVD Score: 5.205	Right Half-Site RVD Score: 6.909
	Genomic Region: Intron	Closest Gene: NELL1
Match Type: R24R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GCATC TGCACATAACCACTGT          TGGGTTGCCTGTTGTCATGAAGTG          TCAATGGTAAGGTGCA GTTTA-3'
	3'-CGTAG ACGTGTATTGGTGACA          ACCCAACGGACAACAGTACTTCAC          AGTTACCATTCCACGT CAAAT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 011-020782555
Forward Primer: 5'-CATGAGTGTTCAAGCTGCTGCC-3' Tm: 59.258
Reverse Primer: 5'-GTAGCCTTGAACTGTGACTGCTTG-3' Tm: 59.038
Expected PCR Product Length: 331
Expected CelI Cleavage Product Sizes: 168, 163
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr11:20782693-20782748

Rank: 195	Homology Score: 5	RVD Match Score: 3.078	Left Half-Site RVD Score: 5.619	Right Half-Site RVD Score: 6.452
	Genomic Region: Intergenic	Closest Gene: KHDRBS3
Match Type: R30R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TTTGT TAAACACCACTCCTGT       GCCATGTAGGAGAACACAAGGGTGTGGATG       CCAGGAGGCAGGGCCA CGGAG-3'
	3'-AAACA ATTTGTGGTGAGGACA       CGGTACATCCTCTTGTGTTCCCACACCTAC       GGTCCTCCGTCCCGGT GCCTC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 008-136674744
Forward Primer: 5'-GGAAGGAGAGTCCATGTGAGG-3' Tm: 59.378
Reverse Primer: 5'-CCCTTGAGGTAGGCAGTACTATC-3' Tm: 59.148
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 135, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr8:136674846-136674907

Rank: 196	Homology Score: 2	RVD Match Score: 3.079	Left Half-Site RVD Score: 6.686	Right Half-Site RVD Score: 5.411
	Genomic Region: Intergenic	Closest Gene: TRIM56
Match Type: R18R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GCCAC TGCACTTGGCCCATAT             CATGCAGTATTAAGGGGC             ATAGGAGGAAAGTGTA GACCC-3'
	3'-CGGTG ACGTGAACCGGGTATA             GTACGTCATAATTCCCCG             TATCCTCCTTTCACAT CTGGG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 007-100750568
Forward Primer: 5'-GAGGAGGGATCTCATTGTGTTACC-3' Tm: 59.038
Reverse Primer: 5'-GAGACCAGCCTGGCCAACAT-3' Tm: 59.508
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 132, 138
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr7:100750673-100750722

Rank: 197	Homology Score: 5	RVD Match Score: 3.079	Left Half-Site RVD Score: 7.082	Right Half-Site RVD Score: 5.067
	Genomic Region: Intergenic	Closest Gene: RNGTT
Match Type: R28R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TGGGC TGGAGCTGAGTCCCAT        AGCCGCCTGTTGGGGGGTGATGGCCCCC        ACAGGAGGGAGCTGTA AAATC-3'
	3'-ACCCG ACCTCGACTCAGGGTA        TCGGCGGACAACCCCCCACTACCGGGGG        TGTCCTCCCTCGACAT TTTAG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 006-089284033
Forward Primer: 5'-GCGCCTGGCTAAATGAGGCT-3' Tm: 59.508
Reverse Primer: 5'-GGTGTTGGTGGCCATTACGAG-3' Tm: 59.378
Expected PCR Product Length: 316
Expected CelI Cleavage Product Sizes: 146, 170
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr6:89284147-89284206

Rank: 198	Homology Score: 10	RVD Match Score: 3.08	Left Half-Site RVD Score: 5.893	Right Half-Site RVD Score: 6.179
	Genomic Region: Intergenic	Closest Gene: KCNT2
Match Type: R10R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CAGAT TGGACGTGACTACTGT                 GCCCTTGCCC                 AGAGGAGTCCTATGTA TCCAT-3'
	3'-GTCTA ACCTGCACTGATGACA                 CGGGAACGGG                 TCTCCTCAGGATACAT AGGTA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 001-195936317
Forward Primer: 5'-GGACCATCAGCAGATAGTACGAAG-3' Tm: 59.038
Reverse Primer: 5'-GTTGGGGTCAGTTTCCTAGGC-3' Tm: 59.378
Expected PCR Product Length: 383
Expected CelI Cleavage Product Sizes: 251, 132
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr1:195936545-195936586

Rank: 199	Homology Score: 8	RVD Match Score: 3.08	Left Half-Site RVD Score: 6.237	Right Half-Site RVD Score: 5.84
	Genomic Region: Intergenic	Closest Gene: MIR4790
Match Type: R19R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GGATT TGAACCTGGCTTCTCT            GCCACCTGCATTTTTGACC             ATGGGAGCCAGGAGCA GGGCG-3'
	3'-CCTAA ACTTGGACCGAAGAGA            CGGTGGACGTAAAAACTGG             TACCCTCGGTCCTCGT CCCGC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 003-005393709
Forward Primer: 5'-CATCTACAGAATAACCCCAGGAGG-3' Tm: 59.038
Reverse Primer: 5'-CCGTGTCACAGACAGTGTCTGAA-3' Tm: 59.148
Expected PCR Product Length: 310
Expected CelI Cleavage Product Sizes: 140, 170
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr3:5393822-5393872

Rank: 200	Homology Score: 13	RVD Match Score: 3.082	Left Half-Site RVD Score: 5.289	Right Half-Site RVD Score: 6.847
	Genomic Region: Intron	Closest Gene: ACSS3
Match Type: R12R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TTGCA TGCACCCTAGTCCTGT                TGTGTGTTAATA                ACAGGCATCAATTGCA TTTTC-3'
	3'-AACGT ACGTGGGATCAGGACA                ACACACAATTAT                TGTCCGTAGTTAACGT AAAAG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Warning! Primer pair was generated using suboptimal parameters. 
Primer Index: 012-081520008
Forward Primer: 5'-GGCAGCAACTCTCCAGTGAGTA-3' Tm: 59.258
Reverse Primer: 5'-CCTGTATTTCCCTATGTATATGTGCTCAGTC-3' Tm: 59.958
Expected PCR Product Length: 413
Expected CelI Cleavage Product Sizes: 171, 242
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr12:81520155-81520198

Plate format Oligo Order Form
Well
Name
Sequence
5' mods
Internal
Notes
Primer Plate #1
A1
011-005248069F
GTGCCTATCAGAAACCCAAGAGTC
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
A2
011-005248069R
CAATCTACTCCCAGGAGCAGG
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
B1
011-005255477F
GTCAGTGCCTATCAGAAACCCAAG
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
B2
011-005255477R
TCACTGGAGCAGGGAGGACA
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
C1
00X-034076224F
AGTATTGCCCTTTCTGCTAGGGAC
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
C2
00X-034076224R
CACTGCACACTTCTGTGGCTC
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
D1
022-045287459F
CAAAGGCTCTGCCTGCAGGA
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
D2
022-045287459R
CAGGACTAAGGCCGCTCTTC
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
E1
006-110284189F
GCAATGTTGCTCCAAGAGGCC
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
E2
006-110284189R
GAACTTATTCCTCCCACCTAGCTG
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
F1
003-058640715F
CATATGAGGAAACTGAGAGGCGG
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
F2
003-058640715R
CCTCGAGGAGGGCTATTCTAGA
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
G1
dom-000216374F
TCTCCTTTCTGTACCTCAGAGACC
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
G2
dom-000216374R
CCCACCCTGGTACAGCAAATG
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
H1
005-059619945F
CCAAGACTTAGCTTAGCACCCAAC
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
H2
005-059619945R
CATGCCTGGAGTTAGCCTCTCT
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
A3
014-077327823F
CCAACAGCTGGTAAGGATGTCAAG
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
A4
014-077327823R
CTGTGGAAACACCAAAGCAGGG
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
B3
005-177943719F
AAGCTTTCTGCCTCCTCAGGCC
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
B4
005-177943719R
CACCTCAGTGACAGGCGCTT
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
C3
012-040234817F
CCTTACAATTCACGGCTCTCAGAC
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
C4
012-040234817R
CCACTGGAGTTGACAGATTTGGC
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
D3
011-002239013F
AGCTGTCCAGCCTGCAGCAT
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
D4
011-002239013R
TCATATTCCTGGGTGGTCACTCC
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
E3
00X-079108050F
GGTGTCAGTAGCAGATAGGGATG
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
E4
00X-079108050R
CTGTACCCAACCCACTTTATGGC
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
F3
002-011376315F
CCCCTCATGGAGAACACAATACAAAAGAAAC
S2_S5_NN_Ranking-RVDs_5T	Warning! Primer pair was generated using suboptimal parameters. 
F4
002-011376315R
GAGAGAAGAGTTACGCGTCTTCC
S2_S5_NN_Ranking-RVDs_5T	Warning! Primer pair was generated using suboptimal parameters. 
G3
016-071012605F
CTCCTAGCTTCCTGATTCTTTGCC
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
G4
016-071012605R
CCCACCCTGGTACAGCAAATG
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
H3
019-045727578F
GAGAAGGGGAGTGTTCCACTG
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
H4
019-045727578R
GGATAACAGAGGGCCATCAGC
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
A5
010-095287016F
GAGCTGCTAAAATTGCTTGCTGCC
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
A6
010-095287016R
GCACCCAGCCTGTTCAGCAA
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
B5
No PrimersF
No Primers
S2_S5_NN_Ranking-RVDs_5T	No Primers
B6
No PrimersR
No Primers
S2_S5_NN_Ranking-RVDs_5T	No Primers
C5
005-078702362F
GAAAGGATCCTGGATGAGGGG
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
C6
005-078702362R
GTAGGAGTTTCCTGCCTCTGTC
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
D5
014-077327606F
GCCTGGTATAGAACAAGCACCTG
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
D6
014-077327606R
CCTCCTGTGATTGCCAGAGC
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
E5
009-134936820F
GCTGGTAAGTGAAGTTGATGTGGC
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
E6
009-134936820R
CCAGGAGCAGCTGCGATGAA
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
F5
003-054731501F
CCTACAGTTTCCAAGGACAAGCAG
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
F6
003-054731501R
CCCAGCAGTAGCTTTAGCATCC
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
G5
004-002790263F
ATGCAGAGATGGCCCAGAGC
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
G6
004-002790263R
TGAGTAGGGCCAAGGCCACA
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
H5
002-130879063F
AAGACGCCCACCGAGCCCA
S2_S5_NN_Ranking-RVDs_5T	Warning! Primer pair was generated using suboptimal parameters. 
H6
002-130879063R
GCTAATGGGTGCAAAGTTTCTTTTAAGGAGCATA
S2_S5_NN_Ranking-RVDs_5T	Warning! Primer pair was generated using suboptimal parameters. 
A7
002-131974522F
GCTAATGGGTGCAAAGTTTCTTTTAAGGAGCATA
S2_S5_NN_Ranking-RVDs_5T	Warning! Primer pair was generated using suboptimal parameters. 
A8
002-131974522R
AAGACGCCCACCGAGCCCA
S2_S5_NN_Ranking-RVDs_5T	Warning! Primer pair was generated using suboptimal parameters. 
B7
001-109847447F
GCAGGCTTGCCAAACTAAAAGCTC
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
B8
001-109847447R
TCAGAGAGCCAACAGAGCCC
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
C7
004-118702135F
GCAGGGACACTGGTGTTATGTCT
S2_S5_NN_Ranking-RVDs_5T	Warning! Primer pair was generated using suboptimal parameters. 
C8
004-118702135R
GATAGTAACTTATTTAGAATTGAGGTTGTGTGATGGAG
S2_S5_NN_Ranking-RVDs_5T	Warning! Primer pair was generated using suboptimal parameters. 
D7
003-060011649F
GTACTGCCAGAGTGTCAAGAAAGC
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
D8
003-060011649R
TAGGAAGAGGACAGTAGGCTAGG
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
E7
003-138953648F
CTTGGTGTCTGAGTGGCAAAACTG
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
E8
003-138953648R
TATGGGCCTGTTTTACACGGGCCT
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
F7
014-024242484F
CAAGTCTTCCTCAGCCATCAGACA
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
F8
014-024242484R
CCAGACACATCAACAGAGTGGAAG
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
G7
001-153175860F
CTCATAAGTGGAGGTCATTTCCCC
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
G8
001-153175860R
GCACACACCAGTTCTTACCACTGA
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
H7
00X-148727617F
GAGTCTTCCATAGCTGAGTGCAAC
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
H8
00X-148727617R
ACCCAACATCTGCCATCTGCAAG
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
A9
005-166596052F
CGTTGCCCAGCTTGATGCCT
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
A10
005-166596052R
GGAAGTTAGGCCCTAGGACC
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
B9
005-137519020F
GCGAGGATCAAAATGGCAATCCC
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
B10
005-137519020R
GGCAGAGGACTAATGTTTCCTGG
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
C9
003-030699112F
CTGGCACAGAGTGAGCACTTAATG
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
C10
003-030699112R
GGTCGTCATGAGGAGAACACCTT
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
D9
011-012966658F
TGAGGGATAGAGCAGAGGATGG
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
D10
011-012966658R
GGGGGAATGATGTCACTGGAAC
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
E9
017-009569404F
GGGAATGAAAAGGACAGCAGGAC
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
E10
017-009569404R
CGGCCCTGCGCGTTATTTCT
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
F9
006-088451104F
GATCTCCTTGAAGGCACATGCAG
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
F10
006-088451104R
GGCCTGGGAAACATAATGACACC
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
G9
019-017971876F
CTCAATTAATCCTCCCTGGAGACC
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
G10
019-017971876R
ATTCGCATGCGGTAGAGGGG
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
H9
001-024242432F
TCCCAAGAGCAAGACATTGGCAG
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
H10
001-024242432R
GCAATGGAGGACCAGGCGTT
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
A11
003-058824763F
GCCAACTGATATATCTGGAGTGGG
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
A12
003-058824763R
TGCCAGGCCTTCCACAAGGT
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
B11
001-045422945F
GCCCCCAAACACCACAAAGTC
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
B12
001-045422945R
ATTACAGGCACATGCCACCACG
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
C11
004-190659833F
CTGCCTCCAATCCCTCAGAAAATG
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
C12
004-190659833R
CTGGTATTGGGTCCAGACCAC
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
D11
002-104369295F
AGGAAGGGAAACTTCACCTCCTG
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
D12
002-104369295R
CCACCACCACAGTCAAGATGC
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
E11
002-235805535F
ACCTCCACCAGACCACGAATG
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
E12
002-235805535R
GACCCTTAGCAGGTCACACTC
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
F11
017-009569404F
GGGAATGAAAAGGACAGCAGGAC
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
F12
017-009569404R
CGGCCCTGCGCGTTATTTCT
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
G11
008-041570215F
GGGTGCAGAGAGATTTGCATGTG
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
G12
008-041570215R
TGTCTCCTCCTGTCCCTGCT
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
H11
006-048618042F
CCTCCTGTCTTTATTAGTCTAGCAAGAAGTTTGT
S2_S5_NN_Ranking-RVDs_5T	Warning! Primer pair was generated using suboptimal parameters. 
H12
006-048618042R
GTGGTGTCCTGGATAAAATCCTGAAACAGA
S2_S5_NN_Ranking-RVDs_5T	Warning! Primer pair was generated using suboptimal parameters. 
Primer Plate #2
A1
018-072086920F
GCTGTCACCAGGAAAAACATAAGAGCC
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
A2
018-072086920R
AAGCTGCTGAAATGGGCAGAGG
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
B1
003-010453381F
TGTTGCAGACACTGGAACAGAGC
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
B2
003-010453381R
CGTGAGAGTGGACTTGCAAGTCTG
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
C1
013-073489472F
GCGCTCTGCTTCTAAGACACATTG
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
C2
013-073489472R
CACATGGAAGAGATGATACATGCCATTACC
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
D1
001-227379954F
GAGATCACTCTCACCACCCAAAAC
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
D2
001-227379954R
GGAAACAGCAGAACGTTGTTGGTG
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
E1
004-085199195F
CAGTTCTACTTCAGGGAAGGGG
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
E2
004-085199195R
GGATACAAGTGGAAAAGCACAGCC
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
F1
017-020954067F
CGGGAAAAAGTACCAGATGGAGAC
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
F2
017-020954067R
GACAGAGTGAGACCCTGTCTCAA
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
G1
003-082432463F
GACAGGGAAGAAACTCTGCAAACC
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
G2
003-082432463R
GCACGGATATGCAATAAACTTCTGTTTCCC
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
H1
00X-056339296F
GCAAGAATATTGGCAATACTGTCCCATGTC
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
H2
00X-056339296R
GCAGAGGCCTAGTCTGAAGC
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
A3
011-109690484F
GCAGAGGCCTAGTCTGAAGC
S2_S5_NN_Ranking-RVDs_5T	Warning! Primer pair was generated using suboptimal parameters. 
A4
011-109690484R
AAGCAAGAATATTGGCAATACTGTCCCATGTC
S2_S5_NN_Ranking-RVDs_5T	Warning! Primer pair was generated using suboptimal parameters. 
B3
006-063449762F
TCTGAGGAGACCTTCAGAGGG
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
B4
006-063449762R
ACCACCTCTTGAAGCAGCTCTC
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
C3
007-002962408F
ACAGCGTTTGTCTCCTGGCC
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
C4
007-002962408R
GCCCCTAAGCCTTGCATTCAG
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
D3
016-088575852F
CCATTAGGCACACAGGGAGG
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
D4
016-088575852R
CTGCCTCCTGAACCCACTCT
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
E3
007-158980941F
GCCCAACTACATGCTGGGTC
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
E4
007-158980941R
GTGGCCTATCTTGATGAATGTCCC
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
F3
004-110007835F
GACTGCTGGGAATTGCTCTTTGAC
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
F4
004-110007835R
TCCCTCAACTTCCACTGGGC
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
G3
009-066282880F
ACTACAGGTGCACACCACCATG
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
G4
009-066282880R
GCCTTCCCCAAGTTTGCATTTTCG
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
H3
020-045428486F
GTCTCACTGAGACCTAGGCG
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
H4
020-045428486R
TGGGCCTGGGGAACCAACAA
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
A5
015-090111045F
GCCTTCCTCTCCTCATACCC
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
A6
015-090111045R
TACAGGTGTGAGCCACCACG
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
B5
006-138657515F
GTTCCCGTGCATCAGTCAGC
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
B6
006-138657515R
GCTGATCAGTGGTTGGAAAGGC
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
C5
018-055555293F
TCTGGGAAGAAGCTGCCAGG
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
C6
018-055555293R
GGCTATGCTACCTGCTGTAAAACC
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
D5
00X-106025404F
CTTTCCCCAACTGTGTGATACAGC
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
D6
00X-106025404R
CTGGTACTGGAACTCTGCTCTTCA
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
E5
004-190407488F
CCTGGGGGAACTTTTTCAGTCCAT
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
E6
004-190407488R
GGTTTCACATGCCCTGAATACTGC
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
F5
019-009837811F
AATCCCAGGTAGCGTACGTGC
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
F6
019-009837811R
TCAGTACACATGGACCTGGCTTC
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
G5
005-143474515F
CCACTCTCCCTCAAATCAACTGC
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
G6
005-143474515R
CTGCATTTCTGAGACAGACATGGC
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
H5
003-102226111F
CCAGCATCATTGAAAGCTGGCAGA
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
H6
003-102226111R
CTGTATCCCCATCATGTGCTGAAG
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
A7
002-170227380F
GGCCAGAAGACCAAGATGATTCC
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
A8
002-170227380R
GCCAAAAGCCAAATGTAGACTTTGTGCTG
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
B7
002-241334168F
CATGCACCCCTGGCTTGTGT
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
B8
002-241334168R
AGCTTTGCACCTGGAACCTGTGA
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
C7
004-068122758F
CTTTTGCTTGTTGGTTCGGTGGC
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
C8
004-068122758R
GTCCTGATGGAGTCTAAATGGCTG
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
D7
008-011765812F
ATCCCCAGTAATGTACCTGCTGTC
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
D8
008-011765812R
CTCAGTACACTTGCACCCGG
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
E7
008-142885698F
GTGAACTACCTCTCTGGCACTG
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
E8
008-142885698R
CTCACCCAAAACTGGAAGCAACC
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
F7
022-024555541F
CACAGCCATTCTGTCTACTCCC
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
F8
022-024555541R
TGGCAGGTGGCCAGTGTCTT
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
G7
004-024170953F
CTGGATGGGATCCCAGAACAGA
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
G8
004-024170953R
GAGCCAACATGCTTGGCTGAG
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
H7
011-117929965F
GCTCCGATGTGAGAAGTCTCTAG
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
H8
011-117929965R
CCTTCCACACATTCTGCACTCTG
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
A9
022-036626609F
CTGACTCCAGGCTCTGACAC
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
A10
022-036626609R
GCTGGGGCCTTTTTTCTCATCTG
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
B9
016-030831779F
CACACACACACACACATGGGC
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
B10
016-030831779R
ACTGTCTGGGGAAGGGATGG
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
C9
021-043483081F
TGGAGGCTGCGAGGGAGAAA
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
C10
021-043483081R
GCACCCCTGCAAAAGATATGTCC
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
D9
006-153846994F
GCAAGCTGTCTCATGCTCACTG
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
D10
006-153846994R
CAGGAAGGAACTTGTCAGATTCCC
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
E9
004-170092193F
GACCTAAAGGTTTCTCCACACATAGTGAAG
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
E10
004-170092193R
GACTGTTCTGAGATCTTGGGTGTG
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
F9
013-100940227F
GTGGTAGACCTTCAGAATTTTATGAGGGAG
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
F10
013-100940227R
TCACCACTGCACTCCAGCCT
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
G9
007-044570049F
GGAGATGGATGGTGGTGATAGTTG
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
G10
007-044570049R
TTAGGCAGTACTGGCCTGGG
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
H9
001-231353429F
AAGGCTGTGGAGCCTCCACCAT
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
H10
001-231353429R
AATCCCAGCCCCCAAGCTCA
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
A11
010-052784032F
ACTGAGGCCCAAATCATAGCCAATGATGGA
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
A12
010-052784032R
AGGCCTCCACAGCCATGCTT
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
B11
010-107873570F
GACACCTTCTGCTAAGCACACATC
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
B12
010-107873570R
GAGCTCATTCAAAGCCCCAGTAG
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
C11
002-191816679F
CCAACAGTTTGGGAGGCCAAG
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
C12
002-191816679R
CTGCCCAGAGTCCAGAACAGT
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
D11
011-031965225F
CCAGCCTGAGACAACCATCTTTAC
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
D12
011-031965225R
GAAGGGCCATAACTTGGGCATTAG
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
E11
001-213140398F
ATTCTAAGAGTGGCGTGTGGAGG
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
E12
001-213140398R
GTTGTGGGTGATTCCAATGGCATG
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
F11
001-230976433F
GCGCCAAGGTCTTCTTGAGC
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
F12
001-230976433R
GGAGAGTAGGAGACAGCACATG
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
G11
003-004440767F
TATGAGAACGAGAGGGGGCG
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
G12
003-004440767R
CAAGCCCTTCCCCACTGTAG
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
H11
009-009921803F
CAGCTGATATTATGTGTCCCACACTGTTC
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
H12
009-009921803R
GAGTTGCTCACAGCTTCTCTGAGT
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
Primer Plate #3
A1
010-131523914F
CTCGGAGGTCTGTCCATGCT
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
A2
010-131523914R
GAGCTAGAAGGGGCTGGTTG
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
B1
010-045378787F
GGTGAGGCTCCATCCCACAA
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
B2
010-045378787R
ACCCAGAGAGGGTGTGAGAG
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
C1
022-024444126F
TGCTCTCTCCTCCCAAGCAC
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
C2
022-024444126R
GGTCTCACCAGTGAACACACAG
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
D1
004-043460367F
GCTAAGAGCCTCTTGGGCTTTTG
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
D2
004-043460367R
AGCAGAGGCAGAAAGGACCAG
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
E1
008-142398434F
GCCTGAGATAGGGACATTGGTG
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
E2
008-142398434R
CCCATTCTGAGCGAGCTTGGA
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
F1
009-019336805F
GTCCATTGATAATCACTCTAGCACAGGTAC
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
F2
009-019336805R
GTGTGACTTCCTTCCTTCCCTC
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
G1
001-230749531F
CAAAGATGCAAAGGCTGATTTAGAGCACC
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
G2
001-230749531R
CCCCAAATTTAGCAATACTGTCTGCTGGA
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
H1
011-056386094F
GGGAGGTACTATGCCTGGATG
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
H2
011-056386094R
GCTCGGGAGGATTAGTAAAAGAGG
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
A3
002-028093265F
GCTGCCAGTGGTGACTGTTTTTC
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
A4
002-028093265R
GTGAGTCTACACACCATGTCATCC
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
B3
004-188278629F
CCTTTCCCTTACCCTGTTTGTTCC
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
B4
004-188278629R
GTGTCCTAGGAACACTGCTCTG
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
C3
017-003965044F
CGTAAGTCCTGACAACTCTACCTC
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
C4
017-003965044R
GAAAGGCAGGACCCTTATCACTGT
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
D3
020-034535532F
CTCTGCTGCTCAACATGAAACTGG
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
D4
020-034535532R
TTCAACGATGTGCACAGAAGAGGG
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
E3
002-219751327F
TGTGTCTTAACGAGCCGCCC
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
E4
002-219751327R
CTGACAGCTGTCATAGCCACG
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
F3
008-012610456F
GCCTCTCTACCAGTTAACAGTGTG
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
F4
008-012610456R
CGAAGCACAAACCCCGGAGA
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
G3
007-126235510F
GGGGTTTTGTCATGTTGCCCG
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
G4
007-126235510R
GCGACCAGCCGGACTATAAATC
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
H3
004-186747672F
CTGTGAGCTACTGAGGATATGCG
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
H4
004-186747672R
GGGCAGATGCTTAAATCACTCCCA
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
A5
005-016519335F
CCTAGATCAGCGGCTTTCAAAGCA
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
A6
005-016519335R
GGGCTGAAAGGAGCTTTGAGG
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
B5
005-083704636F
GGAACGGATTAAAGGGAAGCCAG
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
B6
005-083704636R
GGTCTGTTTCTGTAACATCAGAGGCAAAG
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
C5
011-027668392F
GGTGGGTCCATTCTGACTCTG
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
C6
011-027668392R
TCATGATCCACCCACCTCGG
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
D5
001-146791343F
GACTGCCTGCCTTAAAAAGCTTGG
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
D6
001-146791343R
ATCTCTGGGATTGGGCCTGG
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
E5
007-003795640F
CCTGACTCCTTTCACATGGCATAG
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
E6
007-003795640R
TGTGTCACACGTGCTATGCCATG
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
F5
007-003795876F
GAGTCCTTTCACATGGCATAGCAC
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
F6
007-003795876R
ACATGTCTCACACGCACAATGCC
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
G5
016-054900620F
GGATCTAGCCCTTGAGTGTGG
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
G6
016-054900620R
GGGGTTTCACCATGTTATGTTGGC
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
H5
014-086334283F
CACACACACACACACCCTTTGATC
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
H6
014-086334283R
CAGCAACGCTGACCTAGAAACTC
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
A7
012-120352694F
CGGGTCTGTTTAGAGAGAGGG
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
A8
012-120352694R
GGCCTCCCAAAATGCTGAGATTAC
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
B7
018-074790610F
GAGCTGTGGACTGAAGAGCAAC
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
B8
018-074790610R
CACAGTCTTGGGAGGGTGCT
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
C7
013-067512369F
GGCATCAACAGGGCTTCTTAAACG
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
C8
013-067512369R
CAATGTCAGTCGTTTCTGCATGAAGATAGG
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
D7
005-056491247F
GTGTACCACGTCTGGCCTAG
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
D8
005-056491247R
GCGCCCAGCCGAAAAACGTA
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
E7
016-087916217F
TGGGCCCAACATGTCAGTGC
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
E8
016-087916217R
GAGATCACACTTGGTGGCGATG
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
F7
018-076246512F
AGCCTCCTACTGATGGGTGG
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
F8
018-076246512R
GCTGATGAAGAAGAGGATGGGG
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
G7
006-008406248F
GTACACGACCTGCTCTATGGC
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
G8
006-008406248R
GTGCTTGAGATGTCAGTGGCAG
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
H7
001-205306079F
GGTTTGGAACCCCCAATCATTCTC
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
H8
001-205306079R
GGGGCTCTAAGTTGACCTCTC
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
A9
005-056444609F
GAGCGATAGAGCAAGACTCTGTCA
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
A10
005-056444609R
GGGAATACAGGTGCCCATCACT
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
B9
001-116812599F
GACTGGACTCAGCTGAGTGG
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
B10
001-116812599R
CCTGGAGCACTTGGTGGCAT
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
C9
017-068268613F
AGGAAGGGATTCTCCTAATAAATTGGCAGATG
S2_S5_NN_Ranking-RVDs_5T	Warning! Primer pair was generated using suboptimal parameters. 
C10
017-068268613R
CTATGGTCCATGTCTAACGTTAATACTACATGC
S2_S5_NN_Ranking-RVDs_5T	Warning! Primer pair was generated using suboptimal parameters. 
D9
016-059193695F
GTAGCCTCTCCCAATACTTAACGG
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
D10
016-059193695R
CCTGTGTTGTTTCTGTCCAAGGC
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
E9
006-083995632F
CAGTTGTTCGGATACGGCTTATCTTCATG
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
E10
006-083995632R
GGGTAAGAAAACAGAGGCACAGAG
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
F9
013-072629328F
GCCAAGCACTGTCTGGAATAACTC
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
F10
013-072629328R
CTGGGCTTATTGCATTGTGGGG
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
G9
002-023872858F
GTGGTCTTAGGCACCTCCTG
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
G10
002-023872858R
CTCACTGTCCCCTTAACTTTCCTG
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
H9
020-062498891F
CCTGGGTGACAGAGTAAGACTCG
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
H10
020-062498891R
CCAATATGGTGAAACCCCGTCTC
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
A11
005-004975411F
CTGTAGGAGAAGGCCAGAGAAATC
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
A12
005-004975411R
CCAAAGTGCAGGGATTACAGGC
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
B11
001-038169360F
CCTGGGCAACAGAGTGAGAC
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
B12
001-038169360R
GGAGTGAGACCCTGCCTCAT
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
C11
008-098131709F
GCCCCTTCTTTCCTCACAGAAATG
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
C12
008-098131709R
AATGGCTAGCTGCCAGGTGAC
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
D11
005-172280139F
TGGTTTACCACGTGCTGTTGTTGC
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
D12
005-172280139R
AAGATGCCTCTGGGTGGCCT
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
E11
016-021539209F
CAGCATGTCAGTGCGACCCA
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
E12
016-021539209R
AGGAGATCACACTTGGTGGTGATG
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
F11
022-037324670F
ACCCAGAGTTCCCAGGAGATAG
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
F12
022-037324670R
CTCAGTGGAATTCCCTCCACC
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
G11
002-085687121F
ACTGACATCAGAGGTTTCCCAGAG
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
G12
002-085687121R
GAGTGGGATACATTGACTGGTCTG
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
H11
018-050077032F
GCCCTGGAATAGACAATACTGCTC
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
H12
018-050077032R
CCCTAACATCTGCACATGTACCTC
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
Primer Plate #4
A1
018-011130975F
GGTGTCAGTGGCAGATAGGG
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
A2
018-011130975R
GCTGTGCATGACACGCTTTATGG
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
B1
006-049406871F
GCTGCTGTGCAAGACCCACT
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
B2
006-049406871R
GGTGTCAGTGGCAGATAGCAATG
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
C1
018-050114372F
GTGCATGACCCACTTTATGGCTAG
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
C2
018-050114372R
CTTGAGGTGTCAGTGGTAGTAGG
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
D1
004-133032214F
TGCACCACCCACTTTATGGCTAG
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
D2
004-133032214R
GAGGTGTCAGTGGCAGATGG
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
E1
008-064233767F
TGTCAGTGGCAGATAGGGATGCT
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
E2
008-064233767R
GCTGTGCATGACCCACTTTATGGC
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
F1
00X-080874147F
TGTCACTGGCAGATAGGGATGCT
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
F2
00X-080874147R
GCTGTACACATCCCACTTTATGGC
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
G1
002-230410259F
CAGCCTGGGTGACACAGCAA
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
G2
002-230410259R
AACTTTGCTCTTTGTGGAGCCGG
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
H1
013-098576588F
GGTCATTCCTGGCTGTGCAAC
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
H2
013-098576588R
CTTTGGAAGGGCATAGTGACCTG
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
A3
005-075922611F
AGGGTAGCCCGGGAAGACTTT
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
A4
005-075922611R
TAAAGCTTCCCCAAAGGCTTCCC
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
B3
00X-115065707F
GAAGGCAGGAGCTAAGAAAGGG
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
B4
00X-115065707R
GGACCCCCTTCATGTATCTCTC
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
C3
008-122428182F
ACAAGTTCTGCCAACTACACCAGG
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
C4
008-122428182R
AGAGCCATTCCCAAGTGGGGT
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
D3
008-123811913F
AGGGCTCAGCCTCTGTGACT
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
D4
008-123811913R
TGCAGTGAGCTAAGGTCACACCA
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
E3
013-046233753F
CTCCCTATGTTGTCCAGGCTAGA
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
E4
013-046233753R
GCTGGGTTTGGTAGTTCTAACTCC
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
F3
016-077615266F
TCGCTCTCCAGCATCGACTC
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
F4
016-077615266R
GGTGGAAAGCCATTCACTCCTAG
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
G3
010-102872293F
GTGAGGAGGCCAAGAAAGCTG
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
G4
010-102872293R
CATGTGTGTGAGAACCTGTGTGTG
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
H3
012-038979055F
GCTGGTGAGTGCCCACAAAATTAC
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
H4
012-038979055R
CGCGGTCCCCTAAACCTAATTTC
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
A5
004-182299969F
GACAAGGAAACTGAGGCACAGAAG
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
A6
004-182299969R
ACACTGATTCCTGACCTCAGCG
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
B5
020-033210588F
GATGCACAGGCACTAGGCTTTC
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
B6
020-033210588R
GCGTTTCTGCCTGTCTTGGTATAG
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
C5
006-035782662F
GATGGGGACACCAAGACTAATCAG
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
C6
006-035782662R
TCATCTGCTGTTGTTCCCCATCTC
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
D5
005-101991696F
GTAGACTCTCCATCGTCCTCAC
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
D6
005-101991696R
GGTGACCAGTAATCTCAGGGTCT
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
E5
012-052789060F
ATTCCTCCCACCTCCTGCCA
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
E6
012-052789060R
TTCAGGGCCCAGAAGCCACA
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
F5
00Y-014998617F
ACTACAGGGGCATGCCACCA
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
F6
00Y-014998617R
TTGGAGGCGAGAAGGCAGTG
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
G5
022-016518848F
GCAGCAGTGTTCCGGTATCC
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
G6
022-016518848R
CGGTCACCTAGGATTCCAGAATAC
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
H5
014-019308133F
CCTAGGATTCCAGAATACTGCTGC
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
H6
014-019308133R
AACCTAGCAGCAGTGTTCCGG
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
A7
009-096644130F
CCCCCTTACATAGTGTGGAAGC
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
A8
009-096644130R
TCGGAGTTTCTTCCTTCTGGTGG
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
B7
005-019467940F
GAGATCAGGCCACAGTCATATTCC
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
B8
005-019467940R
CCTGGCGACAGTTGTCATCATC
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
C7
010-125852973F
CCTAACTTGTACCTGAGAAGCCTG
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
C8
010-125852973R
AGTGTTGACTCGGAGGGGGA
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
D7
012-006059468F
GGTAAGAGGAAAGGCGAAGTCATG
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
D8
012-006059468R
CAGGGCCTTAAATCCACAGCAAC
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
E7
004-142620655F
GGGAGCTCCATTGTTAGGTGC
S2_S5_NN_Ranking-RVDs_5T	Warning! Primer pair was generated using suboptimal parameters. 
E8
004-142620655R
CAACTAACATGATGAATAGAACAGTACTTCACATCTC
S2_S5_NN_Ranking-RVDs_5T	Warning! Primer pair was generated using suboptimal parameters. 
F7
001-011023947F
TTTAATCCCCCCTCCCCTCC
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
F8
001-011023947R
AAAGCGTCCACTGTGGGCCCAT
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
G7
005-080195404F
TAGTTAAGGCACCCATTGACTGGC
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
G8
005-080195404R
ACCCAAGACTTCCCCTCGAG
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
H7
003-052584959F
GGCTAGGAATCTTAAGCACCCC
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
H8
003-052584959R
GAGGGGATAGCAAGGCTGTG
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
A9
001-042392691F
TCTGTCTCAGGCGAGGATGTAG
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
A10
001-042392691R
AGGTGGCCTGTCTCAGTGCA
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
B9
018-030409997F
GGTTATGTGGCTGTCTGGTTTACCATTTTC
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
B10
018-030409997R
CCCAGTCACTGTGGTTTCAGTTC
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
C9
020-044517679F
GAGAGCTGCCACAAAGCTGC
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
C10
020-044517679R
GGCTTCGAGGCATAACAAACCC
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
D9
011-077118244F
CCCCCTTCCCCTTTTTGTGTTAC
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
D10
011-077118244R
AAAGCCTAGGGTTGCCACTGAC
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
E9
009-124980262F
CCTCTGGAGCCTGTGACATTC
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
E10
009-124980262R
AGGCTGCTAGGGTTGGGACA
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
F9
001-118086389F
CTGTTCATTTTCTTAAGGTGGAGACTTACCTG
S2_S5_NN_Ranking-RVDs_5T	Warning! Primer pair was generated using suboptimal parameters. 
F10
001-118086389R
GCACTCTGGAAACATTCTCTTTCAATGTGTC
S2_S5_NN_Ranking-RVDs_5T	Warning! Primer pair was generated using suboptimal parameters. 
G9
013-049786521F
TAAAATGTTGGGCTGGGGAAGGAG
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
G10
013-049786521R
GTTTGCCTCCTTGAGCCACC
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
H9
006-101676033F
CCAGGTAACATACTTGCTGTCTGC
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
H10
006-101676033R
GTCACTACTCAGTACACTTGGACC
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
A11
001-233731908F
GTGCTCCTTAATCAGTTCTGTATTCATGTGTC
S2_S5_NN_Ranking-RVDs_5T	Warning! Primer pair was generated using suboptimal parameters. 
A12
001-233731908R
CAGCTAGGTCACTACCAAACTGAG
S2_S5_NN_Ranking-RVDs_5T	Warning! Primer pair was generated using suboptimal parameters. 
B11
001-118423956F
CTCATGGATCTTGCTGGCTCATGT
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
B12
001-118423956R
GTGACTCCAGCGCCTTTGTG
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
C11
006-135592495F
GAGGCTGTTTCAAGGTACAGATGC
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
C12
006-135592495R
GAGGAGGAATCGTATGGAGGG
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
D11
014-050350504F
CCTCCTATCTCGTTATAGCTGGG
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
D12
014-050350504R
GAGGCCTACTGGAAAGTCAGGA
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
E11
005-129459902F
GCAGGACAGTATCTTAAGGGCC
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
E12
005-129459902R
CCTGGGCAACAGAGTGAGAC
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
F11
016-089681728F
CAGCTCCCACTGGGTACAGA
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
F12
016-089681728R
GGGCTCTGAAGACCTGGGAA
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
G11
014-049869550F
CCAGCATTTCCTGTAACTGTGCC
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
G12
014-049869550R
CCAGTTGCTTCCAGGATGTTCCA
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
H11
004-183196263F
CCACCGCACCCAGCAGTATT
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
H12
004-183196263R
CTGAGGGTGAAAGCAAGTACAGTG
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
Primer Plate #5
A1
008-036489761F
GCATCACTGTGGTGAAAACAGAGG
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
A2
008-036489761R
CCGTTATGTGATGGTGGAGGATTC
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
B1
011-020782555F
CATGAGTGTTCAAGCTGCTGCC
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
B2
011-020782555R
GTAGCCTTGAACTGTGACTGCTTG
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
C1
008-136674744F
GGAAGGAGAGTCCATGTGAGG
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
C2
008-136674744R
CCCTTGAGGTAGGCAGTACTATC
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
D1
007-100750568F
GAGGAGGGATCTCATTGTGTTACC
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
D2
007-100750568R
GAGACCAGCCTGGCCAACAT
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
E1
006-089284033F
GCGCCTGGCTAAATGAGGCT
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
E2
006-089284033R
GGTGTTGGTGGCCATTACGAG
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
F1
001-195936317F
GGACCATCAGCAGATAGTACGAAG
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
F2
001-195936317R
GTTGGGGTCAGTTTCCTAGGC
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
G1
003-005393709F
CATCTACAGAATAACCCCAGGAGG
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
G2
003-005393709R
CCGTGTCACAGACAGTGTCTGAA
S2_S5_NN_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
H1
012-081520008F
GGCAGCAACTCTCCAGTGAGTA
S2_S5_NN_Ranking-RVDs_5T	Warning! Primer pair was generated using suboptimal parameters. 
H2
012-081520008R
CCTGTATTTCCCTATGTATATGTGCTCAGTC
S2_S5_NN_Ranking-RVDs_5T	Warning! Primer pair was generated using suboptimal parameters.