PROGNOS Summary for S2_S5_NN_Ranking-RVDs

The hg19 genome was searched for the TALEN pair targeting:
5'- TCACCTTGCCCCACAGGGCAGT NN..NN ACAGGAGTCAGGTGCA -3'

Using these RVDs:
Left:  01HD02NI03HD04HD05NG06NG07NN08HD09HD10HD11HD12NI13HD14NI15NN16NN17NN18HD19NI20NN21NG
Right: 01NN02HD03NI04HD05HD06NG07NN08NI09HD10NG11HD12HD13NG14NN15NG

Up to 5 mismatches were allowed in each nuclease half-site.

Allowed spacing distances were: 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30

Homodimers and heterodimers of the nucleases were included in the output

7329 total sites were located
Mismatch Types
Genomic Regions
  0 and 2: 1
  2 and 2: 1
  3 and 3: 5
  2 and 5: 18
  3 and 4: 26
  3 and 5: 182
  4 and 4: 102
  4 and 5: 1568
  5 and 5: 5426
  Exons: 358
  Promoters: 77
  Introns: 3048
  Intergenic: 3846


The top 200 sites are ranked below according to a weighting of the RVD base preferences.
For the full output, click here.

This output will be deleted from the server one week after it is generated.  Please save your results.

Primer pairs were designed using the 400 bp of sequence on either side of the cleavage site to melt close to 60 °C

For easy visualization of cleavage on gels:
The smallest fragment will be longer than 130 bp, which is also the minimum separation distance between uncleaved and cleaved products.
The two cleavage products will be separated by a minimum size of 0 bp
To optimize assay conditions, the amplicons have a minimum length of 260 bp, a maximum length of 450 bp, and an optimal length of 270 bp.
Rank: 1	Homology Score: 52	RVD Match Score: 2.328	Left Half-Site RVD Score: 3.661	Right Half-Site RVD Score: 4.49
	Genomic Region: Exon	Closest Gene: HBB	Caution! This site scores very closely to the intended nuclease target. The potential for cleavage at this site is of great concern.
Match Type: L16R
	           Left Nuclease
	         TCACCTTGCCCCACAGGGCAGT
	5'-CACGT TCACCTTGCCCCACAGGGCAGT        AACGGCAGACTTCTCC              TCAGGAGTCAGATGCA CCATG-3'
	3'-GTGCA AGTGGAACGGGGTGTCCCGTCA        TTGCCGTCTGAAGAGG              AGTCCTCAGTCTACGT GGTAC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 011-005248069
Forward Primer: 5'-GTGCCTATCAGAAACCCAAGAGTC-3' Tm: 59.038
Reverse Primer: 5'-CAATCTACTCCCAGGAGCAGG-3' Tm: 59.378
Expected PCR Product Length: 308
Expected CelI Cleavage Product Sizes: 154, 154
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr11:5248194-5248247

Rank: 2	Homology Score: 32	RVD Match Score: 2.561	Left Half-Site RVD Score: 5.563	Right Half-Site RVD Score: 4.49
	Genomic Region: Exon	Closest Gene: HBD
Match Type: L16R
	           Left Nuclease
	         TCACCTTGCCCCACAGGGCAGT
	5'-CACGT TCACTTTGCCCCACAGGGCATT        GACAGCAGTCTTCTCC              TCAGGAGTCAGATGCA CCATG-3'
	3'-GTGCA AGTGAAACGGGGTGTCCCGTAA        CTGTCGTCAGAAGAGG              AGTCCTCAGTCTACGT GGTAC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 011-005255477
Forward Primer: 5'-GTCAGTGCCTATCAGAAACCCAAG-3' Tm: 59.038
Reverse Primer: 5'-TCACTGGAGCAGGGAGGACA-3' Tm: 59.508
Expected PCR Product Length: 296
Expected CelI Cleavage Product Sizes: 158, 138
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr11:5255606-5255659

Rank: 3	Homology Score: 17	RVD Match Score: 2.574	Left Half-Site RVD Score: 5.587	Right Half-Site RVD Score: 3.039
	Genomic Region: Intron	Closest Gene: PARD3B
Match Type: R30R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GGAGT AGAAACTGATTCCTAT       GCAAGCAAAAATAACATAGGGGAACTGACC       ACAGGAGTTAGGTGGA GTTAG-3'
	3'-CCTCA TCTTTGACTAAGGATA       CGTTCGTTTTTATTGTATCCCCTTGACTGG       TGTCCTCAATCCACCT CAATC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 002-205984816
Forward Primer: 5'-GTGGGTACTGGCTGCTAAGTATG-3' Tm: 59.148
Reverse Primer: 5'-GTTCCTAGCAGCCACAGCAC-3' Tm: 59.508
Expected PCR Product Length: 428
Expected CelI Cleavage Product Sizes: 256, 172
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr2:205985039-205985100

Rank: 4	Homology Score: 10	RVD Match Score: 2.626	Left Half-Site RVD Score: 3.74	Right Half-Site RVD Score: 5.088
	Genomic Region: Intergenic	Closest Gene: IARS
Match Type: R23R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-ACCCC CGCACCTCACTCCAGT          ATCCCAGGGAGAGAGATTCTCAG           GCAGGAGTCTGTTGTG CTGTG-3'
	3'-TGGGG GCGTGGAGTGAGGTCA          TAGGGTCCCTCTCTCTAAGAGTC           CGTCCTCAGACAACAC GACAC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 009-094951264
Forward Primer: 5'-ATCCAGCCCTGGGACTTTACTTC-3' Tm: 59.148
Reverse Primer: 5'-TGTGAGTCTCAGAACCAAGGCTGT-3' Tm: 59.038
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 135, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr9:94951370-94951424

Rank: 5	Homology Score: 5	RVD Match Score: 2.634	Left Half-Site RVD Score: 4.21	Right Half-Site RVD Score: 4.624
	Genomic Region: Exon	Closest Gene: N4BP3
Match Type: R16R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CTCCC CGCACCCACCTCCTGT              CTCCCTCCCTCCCTCC              ATAGGAGTGGGGGGCC CCTAG-3'
	3'-GAGGG GCGTGGGTGGAGGACA              GAGGGAGGGAGGGAGG              TATCCTCACCCCCCGG GGATC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 005-177549655
Forward Primer: 5'-GGAACAACACCAGGGAATGCC-3' Tm: 59.378
Reverse Primer: 5'-GGGAAATGTTCCCATTCCTTGCTG-3' Tm: 59.038
Expected PCR Product Length: 294
Expected CelI Cleavage Product Sizes: 155, 139
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr5:177549784-177549831

Rank: 6	Homology Score: 10	RVD Match Score: 2.7	Left Half-Site RVD Score: 5.479	Right Half-Site RVD Score: 3.87
	Genomic Region: Intergenic	Closest Gene: FAM47A
Match Type: R10R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-AGTCT TCACCCTTACTCCTAT                 GCTTATTCTA                 ACAGGAGTTAGATGTA CTTCT-3'
	3'-TCAGA AGTGGGAATGAGGATA                 CGAATAAGAT                 TGTCCTCAATCTACAT GAAGA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 00X-034076224
Forward Primer: 5'-AGTATTGCCCTTTCTGCTAGGGAC-3' Tm: 59.038
Reverse Primer: 5'-CACTGCACACTTCTGTGGCTC-3' Tm: 59.378
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 134, 136
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chrX:34076335-34076376

Rank: 7	Homology Score: 10	RVD Match Score: 2.706	Left Half-Site RVD Score: 4.597	Right Half-Site RVD Score: 4.72
	Genomic Region: Intergenic	Closest Gene: PLXNA1
Match Type: R28R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TGACT TGCAGCTCACTCCTCT        GTTCCTCTGCCACCCTGGACAGGACGGC        AGAGTGGTCAGGTGTG CCTCT-3'
	3'-ACTGA ACGTCGAGTGAGGAGA        CAAGGAGACGGTGGGACCTGTCCTGCCG        TCTCACCAGTCCACAC GGAGA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 003-126892267
Forward Primer: 5'-ACCCAGACCCAGACATCCAC-3' Tm: 59.508
Reverse Primer: 5'-CTATGTGCAGCTGGAGAAACTGAG-3' Tm: 59.038
Expected PCR Product Length: 282
Expected CelI Cleavage Product Sizes: 152, 130
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr3:126892387-126892446

Rank: 8	Homology Score: 5	RVD Match Score: 2.71	Left Half-Site RVD Score: 4.624	Right Half-Site RVD Score: 4.72
	Genomic Region: Intergenic	Closest Gene: FAM65B
Match Type: R15R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CAGTT GGCCCCTCACACCTAT              ATGGTAAAGTGTGAA               TGAGGAGTCATGTGTA CACAG-3'
	3'-GTCAA CCGGGGAGTGTGGATA              TACCATTTCACACTT               ACTCCTCAGTACACAT GTGTC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 006-024980600
Forward Primer: 5'-GGGAGTGAAATCAAGACACCTGG-3' Tm: 59.148
Reverse Primer: 5'-AATGCCCAAAAGCCCCCTGTG-3' Tm: 59.378
Expected PCR Product Length: 369
Expected CelI Cleavage Product Sizes: 238, 131
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr6:24980813-24980859

Rank: 9	Homology Score: 2	RVD Match Score: 2.712	Left Half-Site RVD Score: 4.713	Right Half-Site RVD Score: 4.645
	Genomic Region: Intergenic	Closest Gene: NEK10
Match Type: R11R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-AGAGC CACACCTAACTGCTGA                CTGGGATAGGA                 AGAGGAGGCAGGGGTG TGGTC-3'
	3'-TCTCG GTGTGGATTGACGACT                GACCCTATCCT                 TCTCCTCCGTCCCCAC ACCAG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 003-027142836
Forward Primer: 5'-AGGACAGGAAGGAAGAGCCC-3' Tm: 59.508
Reverse Primer: 5'-GTGTGGATTGGCAACCTCTTCATG-3' Tm: 59.038
Expected PCR Product Length: 289
Expected CelI Cleavage Product Sizes: 132, 157
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr3:27142945-27142987

Rank: 10	Homology Score: 18	RVD Match Score: 2.721	Left Half-Site RVD Score: 3.85	Right Half-Site RVD Score: 5.662
	Genomic Region: Intergenic	Closest Gene: PKIB
Match Type: R29R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TTTAG CACACCTGACTGCTGT       GGAGTGTCCCAGCAGAAGGTTGTCCACGT        ACAGGAGCAAGATGCA GCCTA-3'
	3'-AAATC GTGTGGACTGACGACA       CCTCACAGGGTCGTCTTCCAACAGGTGCA        TGTCCTCGTTCTACGT CGGAT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 006-123066634
Forward Primer: 5'-GTAGTGGGATCGTGCCTGTG-3' Tm: 59.508
Reverse Primer: 5'-TCAGTACACTTGGACCCGGC-3' Tm: 59.508
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 135, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr6:123066737-123066797

Rank: 11	Homology Score: 8	RVD Match Score: 2.723	Left Half-Site RVD Score: 3.821	Right Half-Site RVD Score: 5.707
	Genomic Region: Intergenic	Closest Gene: HSBP1
Match Type: R14R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CGGTA CACACCACACTCCTGT               CTCTCGATGCCATT               GGAGGAGGCAGATGCA TCCGG-3'
	3'-GCCAT GTGTGGTGTGAGGACA               GAGAGCTACGGTAA               CCTCCTCCGTCTACGT AGGCC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 016-083864892
Forward Primer: 5'-GGAAACTGTGGCCCAGAGAG-3' Tm: 59.508
Reverse Primer: 5'-CTCAGCATCCGTTCCTGTTCTTC-3' Tm: 59.148
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 135, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr16:83865002-83865047

Rank: 12	Homology Score: 18	RVD Match Score: 2.728	Left Half-Site RVD Score: 4.957	Right Half-Site RVD Score: 4.522
	Genomic Region: Intron	Closest Gene: PHF21B
Match Type: R12R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CATTC TCCACCTGTCTCCTGC                CTCTCAACTGGG                TCTGGAGTCAGGTGGA AAAAT-3'
	3'-GTAAG AGGTGGACAGAGGACG                GAGAGTTGACCC                AGACCTCAGTCCACCT TTTTA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 022-045287459
Forward Primer: 5'-CAAAGGCTCTGCCTGCAGGA-3' Tm: 59.508
Reverse Primer: 5'-CAGGACTAAGGCCGCTCTTC-3' Tm: 59.508
Expected PCR Product Length: 269
Expected CelI Cleavage Product Sizes: 136, 133
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr22:45287571-45287614

Rank: 13	Homology Score: 17	RVD Match Score: 2.731	Left Half-Site RVD Score: 3.407	Right Half-Site RVD Score: 6.305
	Genomic Region: Intron	Closest Gene: ATRN
Match Type: R12R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GCCAC TGCACCTGACCCCTAT                TATCTTCTTTTG                AAATGAGTCATCTGCT AATTC-3'
	3'-CGGTG ACGTGGACTGGGGATA                ATAGAAGAAAAC                TTTACTCAGTAGACGA TTAAG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 020-003459687
Forward Primer: 5'-GCCAGTGTGCCCAGCCAATT-3' Tm: 59.508
Reverse Primer: 5'-GTGTACGTAGCTGTCTTCCCTTC-3' Tm: 59.148
Expected PCR Product Length: 323
Expected CelI Cleavage Product Sizes: 164, 159
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr20:3459827-3459870

Rank: 14	Homology Score: 5	RVD Match Score: 2.737	Left Half-Site RVD Score: 4.189	Right Half-Site RVD Score: 5.379
	Genomic Region: Intron	Closest Gene: ZC3H3
Match Type: R23R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-AGGCA CACACCTGACACCTGC          ACTGGGCAGCACCTTCGAGGCAA           AGGGGAGTGAGCTGCG CTGCG-3'
	3'-TCCGT GTGTGGACTGTGGACG          TGACCCGTCGTGGAAGCTCCGTT           TCCCCTCACTCGACGC GACGC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 008-144606723
Forward Primer: 5'-CTAGCAGCAGGCAAGTCGCA-3' Tm: 59.508
Reverse Primer: 5'-ACACAGAGCATGGGCTTGGG-3' Tm: 59.508
Expected PCR Product Length: 303
Expected CelI Cleavage Product Sizes: 130, 173
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr8:144606824-144606878

Rank: 15	Homology Score: 13	RVD Match Score: 2.737	Left Half-Site RVD Score: 3.87	Right Half-Site RVD Score: 5.759
	Genomic Region: Intergenic	Closest Gene: PLCL1
Match Type: R13R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GCAGA TACACCTGACTTCTAT               TTGAGGCCCAGAT                ACTGGAGACAGGAGCC TTATT-3'
	3'-CGTCT ATGTGGACTGAAGATA               AACTCCGGGTCTA                TGACCTCTGTCCTCGG AATAA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 002-199208411
Forward Primer: 5'-GCCTTACACATAACCTCACTGCC-3' Tm: 59.148
Reverse Primer: 5'-CTCCAACATAACTCAGCCTCGAAG-3' Tm: 59.038
Expected PCR Product Length: 393
Expected CelI Cleavage Product Sizes: 166, 227
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr2:199208553-199208597

Rank: 16	Homology Score: 17	RVD Match Score: 2.745	Left Half-Site RVD Score: 4.174	Right Half-Site RVD Score: 5.455
	Genomic Region: Intergenic	Closest Gene: GPR6
Match Type: R30R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CCATC TCCACCTGGCTCCTGT       GTGGTCCATGTTGCAAGTTTTCAGGAAGGA       GCAGGAGTTAAGGGTA GAGTT-3'
	3'-GGTAG AGGTGGACCGAGGACA       CACCAGGTACAACGTTCAAAAGTCCTTCCT       CGTCCTCAATTCCCAT CTCAA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 006-110284189
Forward Primer: 5'-GCAATGTTGCTCCAAGAGGCC-3' Tm: 59.378
Reverse Primer: 5'-GAACTTATTCCTCCCACCTAGCTG-3' Tm: 59.038
Expected PCR Product Length: 297
Expected CelI Cleavage Product Sizes: 131, 166
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr6:110284287-110284348

Rank: 17	Homology Score: 17	RVD Match Score: 2.747	Left Half-Site RVD Score: 6.039	Right Half-Site RVD Score: 3.708
	Genomic Region: Intron	Closest Gene: PAK4
Match Type: R27R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TTGTC CTCCCCTTGCTCCTGT        CCATTCCGACTCTCACCCATTCTGTGG         ACAGGAGTCAAGTGCT TATTC-3'
	3'-AACAG GAGGGGAACGAGGACA        GGTAAGGCTGAGAGTGGGTAAGACACC         TGTCCTCAGTTCACGA ATAAG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 019-039625781
Forward Primer: 5'-CCTCACCAACATTTGGTACCGTC-3' Tm: 59.148
Reverse Primer: 5'-CGGAGGTTGCAGTGAGCTGA-3' Tm: 59.508
Expected PCR Product Length: 282
Expected CelI Cleavage Product Sizes: 151, 131
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr19:39625901-39625959

Rank: 18	Homology Score: 5	RVD Match Score: 2.759	Left Half-Site RVD Score: 4.256	Right Half-Site RVD Score: 5.47
	Genomic Region: Intron	Closest Gene: C1orf21
Match Type: R18R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-ATTCA TCCACCTCCCTCCTAT             GTGGTGTAATAAACATGT             TCAGGAGTTAGATTCT TGTGA-3'
	3'-TAAGT AGGTGGAGGGAGGATA             CACCACATTATTTGTACA             AGTCCTCAATCTAAGA ACACT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 001-184575055
Forward Primer: 5'-GGTGGTGCTAAACCGAACCTG-3' Tm: 59.378
Reverse Primer: 5'-GCCACTACACCTGGCCAATG-3' Tm: 59.508
Expected PCR Product Length: 389
Expected CelI Cleavage Product Sizes: 157, 232
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr1:184575185-184575234

Rank: 19	Homology Score: 17	RVD Match Score: 2.765	Left Half-Site RVD Score: 3.85	Right Half-Site RVD Score: 5.996
	Genomic Region: Intergenic	Closest Gene: TRHDE-AS1
Match Type: R28R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CCTCC TAGACCTGACTCCTGT        ATTTGTTTCCTCACACTTAAAGGGAACT        CAATGAGTCAGGTGAG TACTG-3'
	3'-GGAGG ATCTGGACTGAGGACA        TAAACAAAGGAGTGTGAATTTCCCTTGA        GTTACTCAGTCCACTC ATGAC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Warning! Primer pair was generated using suboptimal parameters. 
Primer Index: 012-072604585
Forward Primer: 5'-GTTGAGAGTTGACACCAACTTCTTTTCTTTTTCC-3' Tm: 59.668
Reverse Primer: 5'-CCACTCCTAGTGTGGAACCTC-3' Tm: 59.378
Expected PCR Product Length: 393
Expected CelI Cleavage Product Sizes: 263, 130
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr12:72604816-72604875

Rank: 20	Homology Score: 2	RVD Match Score: 2.766	Left Half-Site RVD Score: 4.652	Right Half-Site RVD Score: 5.088
	Genomic Region: Intergenic	Closest Gene: LOC285501
Match Type: R17R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GTAGG GACACCTGACTTCTAC             CACCAGAAGCTGATTTA              ACTGGTTTCAGGTGTG GCCTG-3'
	3'-CATCC CTGTGGACTGAAGATG             GTGGTCTTCGACTAAAT              TGACCAAAGTCCACAC CGGAC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 004-178644255
Forward Primer: 5'-CCCGAAGATGCACTGGCATTAAAG-3' Tm: 59.038
Reverse Primer: 5'-CCGTAGCCATAGAGTGAGGATGGA-3' Tm: 60.908
Expected PCR Product Length: 366
Expected CelI Cleavage Product Sizes: 235, 131
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr4:178644464-178644512

Rank: 21	Homology Score: 2	RVD Match Score: 2.768	Left Half-Site RVD Score: 4.387	Right Half-Site RVD Score: 5.385
	Genomic Region: Intron	Closest Gene: ITPR2
Match Type: R10R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TGAGT AAAACCTAACTCCTGC                 CTTTATGAAG                 TTTGGAGTCTGGTGGA GGAGA-3'
	3'-ACTCA TTTTGGATTGAGGACG                 GAAATACTTC                 AAACCTCAGACCACCT CCTCT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 012-026617507
Forward Primer: 5'-GCACTTGTTGACTATCACAGCCAC-3' Tm: 59.038
Reverse Primer: 5'-CATTCCTGTAGCCCAAGCCTG-3' Tm: 59.378
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 131, 139
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr12:26617615-26617656

Rank: 22	Homology Score: 5	RVD Match Score: 2.772	Left Half-Site RVD Score: 4.66	Right Half-Site RVD Score: 5.125
	Genomic Region: Intergenic	Closest Gene: PRELID2
Match Type: R19R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GACAG AGCCCCTCAATCCTGT            GATTGTAGCATGTTCTTTT             ATTTGAGTAAGGTGTA CAGTT-3'
	3'-CTGTC TCGGGGAGTTAGGACA            CTAACATCGTACAAGAAAA             TAAACTCATTCCACAT GTCAA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 005-144614657
Forward Primer: 5'-CAGTGACCTTAGCAGACTATCAGC-3' Tm: 59.038
Reverse Primer: 5'-CTCTGGGCTTGACAGCAACTGA-3' Tm: 59.258
Expected PCR Product Length: 340
Expected CelI Cleavage Product Sizes: 209, 131
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr5:144614839-144614889

Rank: 23	Homology Score: 10	RVD Match Score: 2.782	Left Half-Site RVD Score: 5.716	Right Half-Site RVD Score: 4.189
	Genomic Region: Intron	Closest Gene: LOC100507605
Match Type: R27R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TCTGG CGCAACTCACCCCTGG        CTTGCACCAAACATTCTTAAAATGTTT         ATTGGGGTCAGGTGCA ATGGC-3'
	3'-AGACC GCGTTGAGTGGGGACC        GAACGTGGTTTGTAAGAATTTTACAAA         TAACCCCAGTCCACGT TACCG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 010-029082810
Forward Primer: 5'-CCTCTGAGCCCCATTCTCCT-3' Tm: 59.508
Reverse Primer: 5'-TAGAGATGGGGTCTCACCTTGTTG-3' Tm: 59.038
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 135, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr10:29082914-29082972

Rank: 24	Homology Score: 10	RVD Match Score: 2.782	Left Half-Site RVD Score: 4.064	Right Half-Site RVD Score: 5.868
	Genomic Region: Intergenic	Closest Gene: FOXP1
Match Type: R22R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GAGCT ATCACCTGACCCCTGT           TAGGATGGCTATTATAAAAAAG           TCAGAAGATAGGTGTA GGAGA-3'
	3'-CTCGA TAGTGGACTGGGGACA           ATCCTACCGATAATATTTTTTC           AGTCTTCTATCCACAT CCTCT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 003-070683485
Forward Primer: 5'-GGGCAAAGGATCTGAATAGACAATTTCCCA-3' Tm: 60.058
Reverse Primer: 5'-CACTGATGGACACTTCTGTTGCC-3' Tm: 59.148
Expected PCR Product Length: 384
Expected CelI Cleavage Product Sizes: 149, 235
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr3:70683605-70683658

Rank: 25	Homology Score: 18	RVD Match Score: 2.782	Left Half-Site RVD Score: 4.867	Right Half-Site RVD Score: 4.984
	Genomic Region: Intron	Closest Gene: FAM3D
Match Type: R17R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CCCTG TGCCCCTGACTCCTTA             CAGCTGTTCTAGAATGT              AAATGAGGCAGGTGCA TTTCG-3'
	3'-GGGAC ACGGGGACTGAGGAAT             GTCGACAAGATCTTACA              TTTACTCCGTCCACGT AAAGC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 003-058640715
Forward Primer: 5'-CATATGAGGAAACTGAGAGGCGG-3' Tm: 59.148
Reverse Primer: 5'-CCTCGAGGAGGGCTATTCTAGA-3' Tm: 59.258
Expected PCR Product Length: 283
Expected CelI Cleavage Product Sizes: 130, 153
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr3:58640819-58640867

Rank: 26	Homology Score: 13	RVD Match Score: 2.782	Left Half-Site RVD Score: 5.228	Right Half-Site RVD Score: 4.632
	Genomic Region: Intergenic	Closest Gene: FAM41AY1
Match Type: R29R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TAGGC TGCATCTTACTCCAGT       ACATAGACAACCTTCTGCTAGGATACTCC        ACAGCAGTCGGGTGTG CAAGA-3'
	3'-ATCCG ACGTAGAATGAGGTCA       TGTATCTGTTGGAAGACGATCCTATGAGG        TGTCGTCAGCCCACAC GTTCT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 00Y-018946879
Forward Primer: 5'-ATACACTTGGACTCGGCTTCCC-3' Tm: 59.258
Reverse Primer: 5'-GTGAATCTCAGGTAGTGTACCTGC-3' Tm: 59.038
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 135, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chrY:18946982-18947042

Rank: 27	Homology Score: 5	RVD Match Score: 2.79	Left Half-Site RVD Score: 4.924	Right Half-Site RVD Score: 4.985
	Genomic Region: Intron	Closest Gene: SH3PXD2B
Match Type: R28R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CTAAC AGCACCTGCTTCCTCT        GCCTCCTCTGCCTCCTCTGCCTCCCAGG        ATAGATGTGAGGTGCT GATGA-3'
	3'-GATTG TCGTGGACGAAGGAGA        CGGAGGAGACGGAGGAGACGGAGGGTCC        TATCTACACTCCACGA CTACT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 005-171849905
Forward Primer: 5'-ATCTTCCACAGGCCAGCCCA-3' Tm: 59.508
Reverse Primer: 5'-GTTTGCCCGGGCTTGTTATCAATG-3' Tm: 59.038
Expected PCR Product Length: 274
Expected CelI Cleavage Product Sizes: 132, 142
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr5:171850005-171850064

Rank: 28	Homology Score: 10	RVD Match Score: 2.791	Left Half-Site RVD Score: 4.423	Right Half-Site RVD Score: 5.523
	Genomic Region: Intergenic	Closest Gene: RPS29
Match Type: R29R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TGTTT TACACATGACTACTGT       TTTATTCCCTCTGTAAGTGGCAGTAATGC        TCAGGAGACAGGTTTC AATTA-3'
	3'-ACAAA ATGTGTACTGATGACA       AAATAAGGGAGACATTCACCGTCATTACG        AGTCCTCTGTCCAAAG TTAAT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 014-049949953
Forward Primer: 5'-GTTGTTCTTTCACCCACTGGAGC-3' Tm: 59.148
Reverse Primer: 5'-GACACATCAGAAAGACCGCTGAC-3' Tm: 59.148
Expected PCR Product Length: 377
Expected CelI Cleavage Product Sizes: 240, 137
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr14:49950161-49950221

Rank: 29	Homology Score: 13	RVD Match Score: 2.795	Left Half-Site RVD Score: 4.977	Right Half-Site RVD Score: 4.965
	Genomic Region: Intron	Closest Gene: RNF103-CHMP3
Match Type: R19R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-AGTGA TCCTCCTGCCTCCTGT            CCCCCAAAATGCTGGGATT             ACAGGTGTGAGCTGCC ATGAC-3'
	3'-TCACT AGGAGGACGGAGGACA            GGGGGTTTTACGACCCTAA             TGTCCACACTCGACGG TACTG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Warning! Primer pair was generated using suboptimal parameters. 
Primer Index: 002-086899302
Forward Primer: 5'-GGCTCTGTCTTTTGTTATGTCAAATGAGAAGCA-3' Tm: 59.758
Reverse Primer: 5'-CTCCATCATTAATTCAAACAGCCTTCAATACCC-3' Tm: 59.758
Expected PCR Product Length: 373
Expected CelI Cleavage Product Sizes: 235, 138
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr2:86899510-86899560

Rank: 30	Homology Score: 2	RVD Match Score: 2.795	Left Half-Site RVD Score: 5.281	Right Half-Site RVD Score: 4.673
	Genomic Region: Intergenic	Closest Gene: CTNNA2
Match Type: R20R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GAGAT GTCCCCTCACACCTGT            TAGAATGCCTATTATCAAAG            ACAGGAGGTAAGTGTT GTAAG-3'
	3'-CTCTA CAGGGGAGTGTGGACA            ATCTTACGGATAATAGTTTC            TGTCCTCCATTCACAA CATTC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Warning! Primer pair was generated using suboptimal parameters. 
Primer Index: 002-080981749
Forward Primer: 5'-GTCTACTGATAGGGAGAAATATTTGCAAACCATG-3' Tm: 59.668
Reverse Primer: 5'-GTGCCAACCATTAACATACAAAGAGAGATTCACA-3' Tm: 59.668
Expected PCR Product Length: 449
Expected CelI Cleavage Product Sizes: 259, 190
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr2:80981980-80982031

Rank: 31	Homology Score: 8	RVD Match Score: 2.799	Left Half-Site RVD Score: 4.262	Right Half-Site RVD Score: 5.767
	Genomic Region: Intergenic	Closest Gene: IRX4
Match Type: R27R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GATGA GGCACCTTAATCCTAT        CTTTGCCACTTAACTTAATCCGTAGTC         ACGGGAGCCACGTGCC ATGGT-3'
	3'-CTACT CCGTGGAATTAGGATA        GAAACGGTGAATTGAATTAGGCATCAG         TGCCCTCGGTGCACGG TACCA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 005-002006653
Forward Primer: 5'-TCAGCCTCCCCAACCTCAGA-3' Tm: 59.508
Reverse Primer: 5'-TGTGGAGATGTCGTTACGCAGG-3' Tm: 59.258
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 135, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr5:2006757-2006815

Rank: 32	Homology Score: 13	RVD Match Score: 2.8	Left Half-Site RVD Score: 4.387	Right Half-Site RVD Score: 5.628
	Genomic Region: Intergenic	Closest Gene: HYDIN2
Match Type: R26R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CCCTC TGCACACAACTCCTAT         GCACTCTGCACGCACCACCCTCCTGC         ACAGGTGTCCCGTGCA GGCTG-3'
	3'-GGGAG ACGTGTGTTGAGGATA         CGTGAGACGTGCGTGGTGGGAGGACG         TGTCCACAGGGCACGT CCGAC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: dom-000216374
Forward Primer: 5'-TCTCCTTTCTGTACCTCAGAGACC-3' Tm: 59.038
Reverse Primer: 5'-CCCACCCTGGTACAGCAAATG-3' Tm: 59.378
Expected PCR Product Length: 318
Expected CelI Cleavage Product Sizes: 131, 187
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr1_gl000192_random:216474-216531

Rank: 33	Homology Score: 5	RVD Match Score: 2.801	Left Half-Site RVD Score: 4.327	Right Half-Site RVD Score: 5.707
	Genomic Region: Intergenic	Closest Gene: COX6A1
Match Type: R26R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TGACA AGCCACTAACTCCTGT         TGAGTGAAGATGTGACCATGTGGTTA         TCAAGAGTCAGGGGGG TTGGT-3'
	3'-ACTGT TCGGTGATTGAGGACA         ACTCACTTCTACACTGGTACACCAAT         AGTTCTCAGTCCCCCC AACCA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 012-120856642
Forward Primer: 5'-CCCTGGAATTGGCTCACTAGCT-3' Tm: 59.258
Reverse Primer: 5'-CTGGGAGGTGAAGGTTGCAGT-3' Tm: 59.378
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 136, 134
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr12:120856747-120856804

Rank: 34	Homology Score: 10	RVD Match Score: 2.803	Left Half-Site RVD Score: 4.064	Right Half-Site RVD Score: 6.031
	Genomic Region: Exon	Closest Gene: TMEM132C
Match Type: R27R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TCGTG ATCACCTGACTCCAGT        TCTCCGTGTGCTCCATTGGCTGCGGCT         GCAGGAGGAAGATGCC TGACA-3'
	3'-AGCAC TAGTGGACTGAGGTCA        AGAGGCACACGAGGTAACCGACGCCGA         CGTCCTCCTTCTACGG ACTGT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 012-129191997
Forward Primer: 5'-GGTGGGGTATACCAGCATAAACTC-3' Tm: 59.038
Reverse Primer: 5'-GGCTTCAAGGACCCTTTCTTAGCT-3' Tm: 59.038
Expected PCR Product Length: 337
Expected CelI Cleavage Product Sizes: 197, 140
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr12:129192163-129192221

Rank: 35	Homology Score: 2	RVD Match Score: 2.805	Left Half-Site RVD Score: 5.666	Right Half-Site RVD Score: 4.387
	Genomic Region: Intergenic	Closest Gene: C6orf118
Match Type: R16R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CTTTA GGCTCCTCACGCCTCT              GAGTCAACAGGCAAAG              ATGGGAGTTAGTTGCT GGCTG-3'
	3'-GAAAT CCGAGGAGTGCGGAGA              CTCAGTTGTCCGTTTC              TACCCTCAATCAACGA CCGAC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 006-165292151
Forward Primer: 5'-GCTCTGATGGCCTAGAGGTC-3' Tm: 59.508
Reverse Primer: 5'-GGGCAGGGTAATACTAAGAGACAC-3' Tm: 59.038
Expected PCR Product Length: 276
Expected CelI Cleavage Product Sizes: 134, 142
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr6:165292259-165292306

Rank: 36	Homology Score: 2	RVD Match Score: 2.81	Left Half-Site RVD Score: 5.552	Right Half-Site RVD Score: 4.521
	Genomic Region: Intergenic	Closest Gene: FBRSL1
Match Type: R29R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TCCTC CACAGATGCCTCCTGT       GGTGTTTCTTGTCATTGCTTCTGGGGGAC        CTAGGAGTAAGGTGTT CTGGA-3'
	3'-AGGAG GTGTCTACGGAGGACA       CCACAAAGAACAGTAACGAAGACCCCCTG        GATCCTCATTCCACAA GACCT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 012-133001318
Forward Primer: 5'-ACACTGCTCAGGAGACCAGG-3' Tm: 59.508
Reverse Primer: 5'-TTACACAGCCCAGCCAAGCC-3' Tm: 59.508
Expected PCR Product Length: 282
Expected CelI Cleavage Product Sizes: 140, 142
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr12:133001426-133001486

Rank: 37	Homology Score: 18	RVD Match Score: 2.811	Left Half-Site RVD Score: 4.571	Right Half-Site RVD Score: 5.505
	Genomic Region: Intron	Closest Gene: PDE4D
Match Type: R29R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CACAC TGCACCTAACATCTGT       CCTTATAATAAACTAGGAGCATCTCTGTT        ACAGGAATCATGTTCA GTAGT-3'
	3'-GTGTG ACGTGGATTGTAGACA       GGAATATTATTTGATCCTCGTAGAGACAA        TGTCCTTAGTACAAGT CATCA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 005-059619945
Forward Primer: 5'-CCAAGACTTAGCTTAGCACCCAAC-3' Tm: 59.038
Reverse Primer: 5'-CATGCCTGGAGTTAGCCTCTCT-3' Tm: 59.258
Expected PCR Product Length: 384
Expected CelI Cleavage Product Sizes: 219, 165
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr5:59620132-59620192

Rank: 38	Homology Score: 2	RVD Match Score: 2.811	Left Half-Site RVD Score: 5.421	Right Half-Site RVD Score: 4.652
	Genomic Region: Intergenic	Closest Gene: CDH8
Match Type: R24R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CTGAT AGCACCAGAATCCTCC          CTCTCTACACCCACATAGCTTTAC          ATAGGAGTCTGATGTT TGAAT-3'
	3'-GACTA TCGTGGTCTTAGGAGG          GAGAGATGTGGGTGTATCGAAATG          TATCCTCAGACTACAA ACTTA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 016-062102796
Forward Primer: 5'-CATTGTAGCCTAGACAAGTCACCG-3' Tm: 59.038
Reverse Primer: 5'-GGGGCAGTGAAGGTATTCAGTATG-3' Tm: 59.038
Expected PCR Product Length: 305
Expected CelI Cleavage Product Sizes: 133, 172
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr16:62102899-62102954

Rank: 39	Homology Score: 5	RVD Match Score: 2.815	Left Half-Site RVD Score: 5.895	Right Half-Site RVD Score: 4.256
	Genomic Region: Intron	Closest Gene: SNX18
Match Type: R30R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TCACA GCCACCGGACACCTGG       GCCCTGCCAGTGCGAGGCAGCGGGAAAACC       ACAGGAGTGAGGCGTG TCCTT-3'
	3'-AGTGT CGGTGGCCTGTGGACC       CGGGACGGTCACGCTCCGTCGCCCTTTTGG       TGTCCTCACTCCGCAC AGGAA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 005-053827309
Forward Primer: 5'-GCCACGTGTTGTCTACAGCAAG-3' Tm: 59.258
Reverse Primer: 5'-CAGCACAAGCACTCTTCGTTATCC-3' Tm: 59.038
Expected PCR Product Length: 368
Expected CelI Cleavage Product Sizes: 238, 130
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr5:53827514-53827575

Rank: 40	Homology Score: 10	RVD Match Score: 2.816	Left Half-Site RVD Score: 5.523	Right Half-Site RVD Score: 4.592
	Genomic Region: Intron	Closest Gene: C14orf166B
Match Type: R24R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TAACT TGCCCCCAACTCATCT          TTTCTTAGATTAGCTCTGGCAATC          ACAGGAGGCAGATGTA ACAGT-3'
	3'-ATTGA ACGGGGGTTGAGTAGA          AAAGAATCTAATCGAGACCGTTAG          TGTCCTCCGTCTACAT TGTCA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 014-077327823
Forward Primer: 5'-CCAACAGCTGGTAAGGATGTCAAG-3' Tm: 59.038
Reverse Primer: 5'-CTGTGGAAACACCAAAGCAGGG-3' Tm: 59.258
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 133, 137
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr14:77327926-77327981

Rank: 41	Homology Score: 10	RVD Match Score: 2.817	Left Half-Site RVD Score: 3.821	Right Half-Site RVD Score: 6.445
	Genomic Region: Intron	Closest Gene: COL23A1
Match Type: R29R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-ATCCC TGCACCTCACACCTAT       CCTGAAGCAAACTAATCAGTAGTTAAATA        AGAGGAGGAGGGTACA TTACG-3'
	3'-TAGGG ACGTGGAGTGTGGATA       GGACTTCGTTTGATTAGTCATCAATTTAT        TCTCCTCCTCCCATGT AATGC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 005-177943719
Forward Primer: 5'-AAGCTTTCTGCCTCCTCAGGCC-3' Tm: 61.288
Reverse Primer: 5'-CACCTCAGTGACAGGCGCTT-3' Tm: 59.508
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 137, 133
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr5:177943824-177943884

Rank: 42	Homology Score: 10	RVD Match Score: 2.819	Left Half-Site RVD Score: 5.066	Right Half-Site RVD Score: 5.046
	Genomic Region: Intergenic	Closest Gene: BTG2
Match Type: R22R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TTCTG TAGGCCTGACTCCTGT           AAGGAGCCTTTGGTAAGGAAGG           TGAGGACTCAGGTGTC CTGGC-3'
	3'-AAGAC ATCCGGACTGAGGACA           TTCCTCGGAAACCATTCCTTCC           ACTCCTGAGTCCACAG GACCG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 001-203281134
Forward Primer: 5'-TGCATGCTGCAGATTAAGAGCCC-3' Tm: 59.148
Reverse Primer: 5'-TAAGAAGTACCAGGACCACAACCC-3' Tm: 59.038
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 137, 133
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr1:203281242-203281295

Rank: 43	Homology Score: 17	RVD Match Score: 2.819	Left Half-Site RVD Score: 6.157	Right Half-Site RVD Score: 4.064
	Genomic Region: Exon	Closest Gene: LOC390660
Match Type: R27R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CTGCC CGCCGCTGCCACCTGT        GGCCCTGTGCCACCTGGCACTCAGGCA         ACTGGAGTAAGGTGCA TGAGG-3'
	3'-GACGG GCGGCGACGGTGGACA        CCGGGACACGGTGGACCGTGAGTCCGT         TGACCTCATTCCACGT ACTCC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 015-082621505
Forward Primer: 5'-TGGCTTTCACGGGTCTGTGC-3' Tm: 59.508
Reverse Primer: 5'-TAGACATGCAGCCTGGAGCC-3' Tm: 59.508
Expected PCR Product Length: 296
Expected CelI Cleavage Product Sizes: 157, 139
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr15:82621631-82621689

Rank: 44	Homology Score: 17	RVD Match Score: 2.819	Left Half-Site RVD Score: 6.157	Right Half-Site RVD Score: 4.064
	Genomic Region: Exon	Closest Gene: LOC390660
Match Type: R27R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CTGCC CGCCGCTGCCACCTGT        GGCCCTGTGCCACCTGGCACTCAGGCA         ACTGGAGTAAGGTGCA TGAGG-3'
	3'-GACGG GCGGCGACGGTGGACA        CCGGGACACGGTGGACCGTGAGTCCGT         TGACCTCATTCCACGT ACTCC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 015-082918832
Forward Primer: 5'-TGGCTTTCACGGGTCTGTGC-3' Tm: 59.508
Reverse Primer: 5'-TAGACATGCAGCCTGGAGCC-3' Tm: 59.508
Expected PCR Product Length: 296
Expected CelI Cleavage Product Sizes: 157, 139
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr15:82918958-82919016

Rank: 45	Homology Score: 17	RVD Match Score: 2.819	Left Half-Site RVD Score: 4.064	Right Half-Site RVD Score: 6.157
	Genomic Region: Intergenic	Closest Gene: LOC642423
Match Type: R27R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CCTCA TGCACCTTACTCCAGT        TGCCTGAGTGCCAGGTGGCACAGGGCC         ACAGGTGGCAGCGGCG GGCAG-3'
	3'-GGAGT ACGTGGAATGAGGTCA        ACGGACTCACGGTCCACCGTGTCCCGG         TGTCCACCGTCGCCGC CCGTC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 015-085803797
Forward Primer: 5'-TAGACATGCAGCCTGGAGCC-3' Tm: 59.508
Reverse Primer: 5'-TGGCTTTCACGGGTCTGTGC-3' Tm: 59.508
Expected PCR Product Length: 296
Expected CelI Cleavage Product Sizes: 142, 154
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr15:85803908-85803966

Rank: 46	Homology Score: 10	RVD Match Score: 2.821	Left Half-Site RVD Score: 5.686	Right Half-Site RVD Score: 4.478
	Genomic Region: Intron	Closest Gene: DSCAML1
Match Type: R23R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-ACGGT GCCATCTGACACCAGT          CCGTGGGTGAGGAGGCGTCTGGG           ACAGGAGTAGGGTGGA TGAAG-3'
	3'-TGCCA CGGTAGACTGTGGTCA          GGCACCCACTCCTCCGCAGACCC           TGTCCTCATCCCACCT ACTTC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 011-117354866
Forward Primer: 5'-GCTCAGGCAAAGTTGTTCCCAG-3' Tm: 59.258
Reverse Primer: 5'-ATGTGCTGAGAGGCTGGTGC-3' Tm: 59.508
Expected PCR Product Length: 286
Expected CelI Cleavage Product Sizes: 130, 156
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr11:117354967-117355021

Rank: 47	Homology Score: 2	RVD Match Score: 2.824	Left Half-Site RVD Score: 4.345	Right Half-Site RVD Score: 5.857
	Genomic Region: Intergenic	Closest Gene: ELAVL4
Match Type: R18R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-AATCA CCCAGCTAACTCCTAT             TGGGCTTCAAGAACTGGT             TCAGGAGTCACCTGTT CCAGG-3'
	3'-TTAGT GGGTCGATTGAGGATA             ACCCGAAGTTCTTGACCA             AGTCCTCAGTGGACAA GGTCC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 001-050729484
Forward Primer: 5'-AACCAGGCCAGGAAAGCCCA-3' Tm: 59.508
Reverse Primer: 5'-GGAGACAGGCACATAGCAGTGA-3' Tm: 59.258
Expected PCR Product Length: 294
Expected CelI Cleavage Product Sizes: 164, 130
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr1:50729621-50729670

Rank: 48	Homology Score: 5	RVD Match Score: 2.824	Left Half-Site RVD Score: 6.505	Right Half-Site RVD Score: 3.821
	Genomic Region: Intergenic	Closest Gene: NACAP1
Match Type: R14R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-AACTT GGGGCCTTACTCCTGA               GAACCTGAACTCAG               AGAGGTGTCAGGTGTC CCTCA-3'
	3'-TTGAA CCCCGGAATGAGGACT               CTTGGACTTGAGTC               TCTCCACAGTCCACAG GGAGT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 008-102329033
Forward Primer: 5'-CCAATACACTGAGCTCCCTCC-3' Tm: 59.378
Reverse Primer: 5'-GAGGAGCAGAACTAGGAACACAG-3' Tm: 59.148
Expected PCR Product Length: 305
Expected CelI Cleavage Product Sizes: 132, 173
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr8:102329140-102329185

Rank: 49	Homology Score: 5	RVD Match Score: 2.828	Left Half-Site RVD Score: 6.591	Right Half-Site RVD Score: 3.783
	Genomic Region: Intergenic	Closest Gene: CYP1B1-AS1
Match Type: R17R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GTGTA TATACTTGAGTCCTCT             TCCCCAGCTTGACACTC              CTAGGAGTCAGGTGTT CTCTG-3'
	3'-CACAT ATATGAACTCAGGAGA             AGGGGTCGAACTGTGAG              GATCCTCAGTCCACAA GAGAC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 002-038352606
Forward Primer: 5'-GCCGCCATGATGAAAACCAAACTC-3' Tm: 59.038
Reverse Primer: 5'-CCCTCTTCCTACTTCTTGGGTG-3' Tm: 59.258
Expected PCR Product Length: 427
Expected CelI Cleavage Product Sizes: 191, 236
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr2:38352771-38352819

Rank: 50	Homology Score: 18	RVD Match Score: 2.829	Left Half-Site RVD Score: 5.662	Right Half-Site RVD Score: 4.551
	Genomic Region: Intergenic	Closest Gene: FLJ11235
Match Type: R29R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TAGGC TGCATCTTGCTCCTGT       ACATGGATGACCTTCTGCTGGGACACTCC        ACGGCAGTCAGGTGCG CAAAA-3'
	3'-ATCCG ACGTAGAACGAGGACA       TGTACCTACTGGAAGACGACCCTGTGAGG        TGCCGTCAGTCCACGC GTTTT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 005-111863094
Forward Primer: 5'-AGTACACTTGGACCCAGCTTACC-3' Tm: 59.148
Reverse Primer: 5'-TGAATCCCAGGTAGCGTACCTGC-3' Tm: 61.088
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 136, 134
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr5:111863198-111863258

Rank: 51	Homology Score: 5	RVD Match Score: 2.829	Left Half-Site RVD Score: 4.11	Right Half-Site RVD Score: 6.181
	Genomic Region: Intron	Closest Gene: TMEM59L
Match Type: R11R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CACTC CTCACCTCACTCCTCT                TAGGCGCCCCC                 AGAGCTGCCAGGTGCT GGTCT-3'
	3'-GTGAG GAGTGGAGTGAGGAGA                ATCCGCGGGGG                 TCTCGACGGTCCACGA CCAGA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 019-018725301
Forward Primer: 5'-GAGGCACAAAGGACAATGTCACC-3' Tm: 59.148
Reverse Primer: 5'-CCATAAGGCAGTGTGTGCGTG-3' Tm: 59.378
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 135, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr19:18725413-18725455

Rank: 52	Homology Score: 5	RVD Match Score: 2.831	Left Half-Site RVD Score: 5.609	Right Half-Site RVD Score: 4.617
	Genomic Region: Intergenic	Closest Gene: GRIN3A
Match Type: R29R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TGTAA CTCACTTGACTTCTGT       CTACATTCTCATTATTCCTGCTAAAAGGA        AGAGGAGTGAAGTGTC TGGCT-3'
	3'-ACATT GAGTGAACTGAAGACA       GATGTAAGAGTAATAAGGACGATTTTCCT        TCTCCTCACTTCACAG ACCGA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 009-105115544
Forward Primer: 5'-CTGAGGTGTATCTAACCCCACCTT-3' Tm: 59.038
Reverse Primer: 5'-CCTTGCTCTAAAGCAGACCTTCAC-3' Tm: 59.038
Expected PCR Product Length: 401
Expected CelI Cleavage Product Sizes: 261, 140
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr9:105115773-105115833

Rank: 53	Homology Score: 13	RVD Match Score: 2.832	Left Half-Site RVD Score: 4.478	Right Half-Site RVD Score: 5.767
	Genomic Region: Intergenic	Closest Gene: RNU6-78
Match Type: R22R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TGAGT ATCACCTCACACCTGT           TAGGATGGCTATTATAAAAAAG           GCAGCAGCCAGGTGCA GTGGC-3'
	3'-ACTCA TAGTGGAGTGTGGACA           ATCCTACCGATAATATTTTTTC           CGTCGTCGGTCCACGT CACCG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 012-032161181
Forward Primer: 5'-GCTCAATGGCAGAAAACCAAATAACCCAAC-3' Tm: 60.058
Reverse Primer: 5'-GAGACGGGTTTTTGCCATGTTGC-3' Tm: 59.148
Expected PCR Product Length: 315
Expected CelI Cleavage Product Sizes: 185, 130
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr12:32161337-32161390

Rank: 54	Homology Score: 10	RVD Match Score: 2.832	Left Half-Site RVD Score: 4.963	Right Half-Site RVD Score: 5.244
	Genomic Region: Intron	Closest Gene: SLC2A13
Match Type: R19R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TAAGA TTCAACTGACTCCTGA            GTTAGATGCAAAAAATTTT             ATAGTAGTGAGGTCTA CTGTT-3'
	3'-ATTCT AAGTTGACTGAGGACT            CAATCTACGTTTTTTAAAA             TATCATCACTCCAGAT GACAA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 012-040234817
Forward Primer: 5'-CCTTACAATTCACGGCTCTCAGAC-3' Tm: 59.038
Reverse Primer: 5'-CCACTGGAGTTGACAGATTTGGC-3' Tm: 59.148
Expected PCR Product Length: 436
Expected CelI Cleavage Product Sizes: 130, 306
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr12:40234920-40234970

Rank: 55	Homology Score: 13	RVD Match Score: 2.832	Left Half-Site RVD Score: 5.345	Right Half-Site RVD Score: 4.867
	Genomic Region: Intergenic	Closest Gene: SGCZ
Match Type: R10R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-ATGGA TGCTCCTGCCACCTGT                 GGAGCCAGCC                 TCAGGAGGCAGGTGAG GGAGG-3'
	3'-TACCT ACGAGGACGGTGGACA                 CCTCGGTCGG                 AGTCCTCCGTCCACTC CCTCC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 008-015147764
Forward Primer: 5'-CCCCACTTCATCTGCTAAACTTCC-3' Tm: 59.038
Reverse Primer: 5'-GAGGGGGAAAATGTGGGAAGTTCT-3' Tm: 59.038
Expected PCR Product Length: 313
Expected CelI Cleavage Product Sizes: 168, 145
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr8:15147909-15147950

Rank: 56	Homology Score: 10	RVD Match Score: 2.833	Left Half-Site RVD Score: 3.821	Right Half-Site RVD Score: 6.578
	Genomic Region: Intergenic	Closest Gene: MIR4686
Match Type: R25R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TCGGC TCCACCTGACTCCTAC         ACTGGCTCTGCAACCATTCCCAGCC          ACAGAGGTTGGCTGCA AGTAC-3'
	3'-AGCCG AGGTGGACTGAGGATG         TGACCGAGACGTTGGTAAGGGTCGG          TGTCTCCAACCGACGT TCATG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 011-002239013
Forward Primer: 5'-AGCTGTCCAGCCTGCAGCAT-3' Tm: 59.508
Reverse Primer: 5'-TCATATTCCTGGGTGGTCACTCC-3' Tm: 59.148
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 138, 132
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr11:2239121-2239177

Rank: 57	Homology Score: 5	RVD Match Score: 2.833	Left Half-Site RVD Score: 4.837	Right Half-Site RVD Score: 5.385
	Genomic Region: Intergenic	Closest Gene: TBX22
Match Type: R28R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TCATC TGCACATAACTACTCT        CCTTTTGAGAAACAGTTCTTGGCCTGTT        ACTGGGGTTTGGTGGA AACTG-3'
	3'-AGTAG ACGTGTATTGATGAGA        GGAAAACTCTTTGTCAAGAACCGGACAA        TGACCCCAAACCACCT TTGAC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 00X-079108050
Forward Primer: 5'-GGTGTCAGTAGCAGATAGGGATG-3' Tm: 59.148
Reverse Primer: 5'-CTGTACCCAACCCACTTTATGGC-3' Tm: 59.148
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 136, 134
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chrX:79108154-79108213

Rank: 58	Homology Score: 5	RVD Match Score: 2.835	Left Half-Site RVD Score: 5.169	Right Half-Site RVD Score: 5.058
	Genomic Region: Intron	Closest Gene: ROCK2
Match Type: R10R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-ACTAC TGCACCTAACTCACAC                 AGAAAAGAGC                 AGAGGAGGTAGGAGCA ATTTA-3'
	3'-TGATG ACGTGGATTGAGTGTG                 TCTTTTCTCG                 TCTCCTCCATCCTCGT TAAAT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Warning! Primer pair was generated using suboptimal parameters. 
Primer Index: 002-011376315
Forward Primer: 5'-CCCCTCATGGAGAACACAATACAAAAGAAAC-3' Tm: 59.958
Reverse Primer: 5'-GAGAGAAGAGTTACGCGTCTTCC-3' Tm: 59.148
Expected PCR Product Length: 307
Expected CelI Cleavage Product Sizes: 169, 138
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr2:11376461-11376502

Rank: 59	Homology Score: 5	RVD Match Score: 2.838	Left Half-Site RVD Score: 5.766	Right Half-Site RVD Score: 4.522
	Genomic Region: Intergenic	Closest Gene: LOC100505622
Match Type: R29R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GTGGA GGAACCCTCCTCCTGT       CACATCATCAGGAGCTGCAGTTGGCTAAC        TGTGGAGTCAGGTGCC TGGAA-3'
	3'-CACCT CCTTGGGAGGAGGACA       GTGTAGTAGTCCTCGACGTCAACCGATTG        ACACCTCAGTCCACGG ACCTT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 019-042664524
Forward Primer: 5'-CAACCCGGTCCAGTCGAGAA-3' Tm: 59.508
Reverse Primer: 5'-TTCCACAGTGGCAGGCACTAG-3' Tm: 59.378
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 131, 139
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr19:42664623-42664683

Rank: 60	Homology Score: 13	RVD Match Score: 2.838	Left Half-Site RVD Score: 4.387	Right Half-Site RVD Score: 5.923
	Genomic Region: Intron	Closest Gene: HYDIN
Match Type: R26R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CCCTC TGCACACAACTCCTAT         GCACTCTGCACGCACCACCCTCCTGC         ACAGGCGTCCCGTGCA GGCTG-3'
	3'-GGGAG ACGTGTGTTGAGGATA         CGTGAGACGTGCGTGGTGGGAGGACG         TGTCCGCAGGGCACGT CCGAC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 016-071012605
Forward Primer: 5'-CTCCTAGCTTCCTGATTCTTTGCC-3' Tm: 59.038
Reverse Primer: 5'-CCCACCCTGGTACAGCAAATG-3' Tm: 59.378
Expected PCR Product Length: 355
Expected CelI Cleavage Product Sizes: 168, 187
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr16:71012742-71012799

Rank: 61	Homology Score: 2	RVD Match Score: 2.84	Left Half-Site RVD Score: 5.559	Right Half-Site RVD Score: 4.72
	Genomic Region: Intergenic	Closest Gene: SALL1
Match Type: R17R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-ATCAA AGAACCTGCCTACTCT             ACACTGACATTTTGTTA              ATGGGAGTCAGGTTGC AGTTA-3'
	3'-TAGTT TCTTGGACGGATGAGA             TGTGACTGTAAAACAAT              TACCCTCAGTCCAACG TCAAT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 016-051386732
Forward Primer: 5'-CCCTCCGAAACAACATCTTACCAC-3' Tm: 59.038
Reverse Primer: 5'-GGGTTGAAGGCAGATATGTTGGG-3' Tm: 59.148
Expected PCR Product Length: 319
Expected CelI Cleavage Product Sizes: 160, 159
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr16:51386866-51386914

Rank: 62	Homology Score: 10	RVD Match Score: 2.84	Left Half-Site RVD Score: 3.85	Right Half-Site RVD Score: 6.599
	Genomic Region: Intron	Closest Gene: LDB2
Match Type: R18R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CTACG AGCACGTGACTCCTAT             AGGTGCAAGCTTTAGAAG             GTAGGAGTCAAGGCCA GGCAC-3'
	3'-GATGC TCGTGCACTGAGGATA             TCCACGTTCGAAATCTTC             CATCCTCAGTTCCGGT CCGTG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 004-016836013
Forward Primer: 5'-CACCTATGTGGCGTGATTGAGTGA-3' Tm: 59.038
Reverse Primer: 5'-AGACAGGGTTTCAGCATGTTGGC-3' Tm: 59.148
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 139, 131
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr4:16836125-16836174

Rank: 63	Homology Score: 8	RVD Match Score: 2.841	Left Half-Site RVD Score: 5.073	Right Half-Site RVD Score: 5.199
	Genomic Region: Intron	Closest Gene: GAA
Match Type: R27R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CGGGG ACCTCATGACTCCTGT        GAGGCTGGGGGGGGTCCTGGCTCACCT         ACAGGCATCAGGTGGC CCAGA-3'
	3'-GCCCC TGGAGTACTGAGGACA        CTCCGACCCCCCCCAGGACCGAGTGGA         TGTCCGTAGTCCACCG GGTCT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 017-078085986
Forward Primer: 5'-AAAAGCGGAGGCCCAGACCA-3' Tm: 59.508
Reverse Primer: 5'-ACTCGGGAACCCTGTCACCA-3' Tm: 59.508
Expected PCR Product Length: 347
Expected CelI Cleavage Product Sizes: 193, 154
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr17:78086148-78086206

Rank: 64	Homology Score: 5	RVD Match Score: 2.841	Left Half-Site RVD Score: 5.767	Right Half-Site RVD Score: 4.543
	Genomic Region: Intron	Closest Gene: RAB11FIP4
Match Type: R14R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GGTGA TCCACCAAACTTCTGC               CCTGCCCTGCCAGA               GCAGGAGGCAGGTGGC ATTGG-3'
	3'-CCACT AGGTGGTTTGAAGACG               GGACGGGACGGTCT               CGTCCTCCGTCCACCG TAACC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 017-029816092
Forward Primer: 5'-GCCAGTTTTCCTTGACTGACCG-3' Tm: 59.258
Reverse Primer: 5'-CCATCCCAGCAACCACTGTAAC-3' Tm: 59.258
Expected PCR Product Length: 273
Expected CelI Cleavage Product Sizes: 143, 130
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr17:29816210-29816255

Rank: 65	Homology Score: 8	RVD Match Score: 2.842	Left Half-Site RVD Score: 6.095	Right Half-Site RVD Score: 4.264
	Genomic Region: Intergenic	Closest Gene: OGFRL1
Match Type: R12R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CCTCC TGCCTCTGCCTCCTGA                GTAGCTGGGATT                ATAGGAGTGAGCTGCT GTGCC-3'
	3'-GGAGG ACGGAGACGGAGGACT                CATCGACCCTAA                TATCCTCACTCGACGA CACGG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 006-071979511
Forward Primer: 5'-GACATTCTAGAGCAGTTTGGCAGG-3' Tm: 59.038
Reverse Primer: 5'-GTGACTGTGGGTGAAGATGCTG-3' Tm: 59.258
Expected PCR Product Length: 334
Expected CelI Cleavage Product Sizes: 174, 160
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr6:71979661-71979704

Rank: 66	Homology Score: 5	RVD Match Score: 2.844	Left Half-Site RVD Score: 4.256	Right Half-Site RVD Score: 6.12
	Genomic Region: Intron	Closest Gene: SLC9A9
Match Type: R19R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-ACACA GCCCCCTCACTCCTAT            GCCTCATGGACAGATGGGT             AGAGGTGTCAGGCACA GTCCT-3'
	3'-TGTGT CGGGGGAGTGAGGATA            CGGAGTACCTGTCTACCCA             TCTCCACAGTCCGTGT CAGGA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 003-143508466
Forward Primer: 5'-GGGCTACTGAGACTCACCATATC-3' Tm: 59.148
Reverse Primer: 5'-GGACTCGGTAACTCACTCAGG-3' Tm: 59.378
Expected PCR Product Length: 315
Expected CelI Cleavage Product Sizes: 163, 152
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr3:143508602-143508652

Rank: 67	Homology Score: 2	RVD Match Score: 2.844	Left Half-Site RVD Score: 4.652	Right Half-Site RVD Score: 5.669
	Genomic Region: Intron	Closest Gene: LAMA3
Match Type: R11R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CCCAG GACACCTGACTTCTAA                ATGTAAATACT                 CCAGGAGGCAGGTATT TCCCC-3'
	3'-GGGTC CTGTGGACTGAAGATT                TACATTTATGA                 GGTCCTCCGTCCATAA AGGGG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 018-021451074
Forward Primer: 5'-CCTTCCCTCTTTCTCACTCACC-3' Tm: 59.258
Reverse Primer: 5'-GGGTCTTCCTTAGGCAGGCT-3' Tm: 59.508
Expected PCR Product Length: 298
Expected CelI Cleavage Product Sizes: 167, 131
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr18:21451218-21451260

Rank: 68	Homology Score: 8	RVD Match Score: 2.846	Left Half-Site RVD Score: 5.759	Right Half-Site RVD Score: 4.58
	Genomic Region: Intergenic	Closest Gene: N/A
Match Type: R20R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CTCAA TGAACTTTACTCCTCT            GAGGAAGGTTAGCAAGAGGG            ATAGGAGTCAGGAGAG GCACC-3'
	3'-GAGTT ACTTGAAATGAGGAGA            CTCCTTCCAATCGTTCTCCC            TATCCTCAGTCCTCTC CGTGG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 215-000126245
Forward Primer: 5'-GTCCCCTCTCCTTGCACTTTTC-3' Tm: 59.258
Reverse Primer: 5'-CCTTGGGAACCTTGGACAATAAGC-3' Tm: 59.038
Expected PCR Product Length: 341
Expected CelI Cleavage Product Sizes: 169, 172
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chrUn_gl000215:126386-126437

Rank: 69	Homology Score: 8	RVD Match Score: 2.846	Left Half-Site RVD Score: 5.759	Right Half-Site RVD Score: 4.58
	Genomic Region: Intergenic	Closest Gene: C21orf15
Match Type: R20R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CTCAA TGAACTTTACTCCTCT            GAGGAAGGTTAGCAAGAGGG            ATAGGAGTCAGGAGAG GCACC-3'
	3'-GAGTT ACTTGAAATGAGGAGA            CTCCTTCCAATCGTTCTCCC            TATCCTCAGTCCTCTC CGTGG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 021-015154271
Forward Primer: 5'-GTCCCCTCTCCTTGCACTTTTC-3' Tm: 59.258
Reverse Primer: 5'-CCTTGGGAACCTTGGACAATAAGC-3' Tm: 59.038
Expected PCR Product Length: 341
Expected CelI Cleavage Product Sizes: 169, 172
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr21:15154412-15154463

Rank: 70	Homology Score: 8	RVD Match Score: 2.846	Left Half-Site RVD Score: 4.911	Right Half-Site RVD Score: 5.399
	Genomic Region: Intron	Closest Gene: ADAMTS2
Match Type: R26R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-ACCCG GGCCCCTGACCCCTGA         CCCCTGGCACTCTGCCCATTGATCCC         AGAGGAGCCAGGTCCT GAGGA-3'
	3'-TGGGC CCGGGGACTGGGGACT         GGGGACCGTGAGACGGGTAACTAGGG         TCTCCTCGGTCCAGGA CTCCT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 005-178559029
Forward Primer: 5'-AACCCTGGCCTCTCCACAAG-3' Tm: 59.508
Reverse Primer: 5'-ATGTTTGAGATCCCTGCAGGAGC-3' Tm: 59.148
Expected PCR Product Length: 272
Expected CelI Cleavage Product Sizes: 142, 130
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr5:178559140-178559197

Rank: 71	Homology Score: 5	RVD Match Score: 2.846	Left Half-Site RVD Score: 5.383	Right Half-Site RVD Score: 4.927
	Genomic Region: Intron	Closest Gene: ROBO2
Match Type: R21R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-ACAGA AACACCTGAAACCGGT           GATCAGAAACTTCTGAATATC            TCAGGAGTTGGGTGAA TGGGC-3'
	3'-TGTCT TTGTGGACTTTGGCCA           CTAGTCTTTGAAGACTTATAG            AGTCCTCAACCCACTT ACCCG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 003-077451553
Forward Primer: 5'-CTGCACGTGTGTCCCAGAAC-3' Tm: 59.508
Reverse Primer: 5'-ACACACGCTCACTTGAGGCATTC-3' Tm: 59.148
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 137, 133
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr3:77451662-77451714

Rank: 72	Homology Score: 5	RVD Match Score: 2.847	Left Half-Site RVD Score: 4.565	Right Half-Site RVD Score: 5.782
	Genomic Region: Intron	Closest Gene: EXOC3L2
Match Type: R16R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-ACCTT TGCACCGAACCCCTAT              AGGGCGGGCACGAGGA              CCAGGAGATGGGTGTA TGGGG-3'
	3'-TGGAA ACGTGGCTTGGGGATA              TCCCGCCCGTGCTCCT              GGTCCTCTACCCACAT ACCCC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 019-045727578
Forward Primer: 5'-GAGAAGGGGAGTGTTCCACTG-3' Tm: 59.378
Reverse Primer: 5'-GGATAACAGAGGGCCATCAGC-3' Tm: 59.378
Expected PCR Product Length: 278
Expected CelI Cleavage Product Sizes: 148, 130
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr19:45727700-45727747

Rank: 73	Homology Score: 5	RVD Match Score: 2.847	Left Half-Site RVD Score: 4.478	Right Half-Site RVD Score: 5.884
	Genomic Region: Intergenic	Closest Gene: NMUR2
Match Type: R23R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-AAGAT ATCACCCCACTCCTGT          TAGAATGGCTACTTAAAGACAAA           ACAAGTGTTAGTTGGA CTCTG-3'
	3'-TTCTA TAGTGGGGTGAGGACA          ATCTTACCGATGAATTTCTGTTT           TGTTCACAATCAACCT GAGAC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Warning! Primer pair was generated using suboptimal parameters. 
Primer Index: 005-152047769
Forward Primer: 5'-GTGGACAAAGAACCTGAATAGACATTTAGCAAAAG-3' Tm: 59.578
Reverse Primer: 5'-GCAGAGTTCCATTTGAGATATTGACTTCATTTCC-3' Tm: 59.668
Expected PCR Product Length: 349
Expected CelI Cleavage Product Sizes: 148, 201
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr5:152047888-152047942

Rank: 74	Homology Score: 10	RVD Match Score: 2.847	Left Half-Site RVD Score: 4.986	Right Half-Site RVD Score: 5.335
	Genomic Region: Intron	Closest Gene: NSMCE2
Match Type: R28R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TGTGC TGCACCTCCCTCGTGT        CATTCTCAAGAATTAAAGTCTTACAGGC        AAAGGACTGAGGTGGC ATATC-3'
	3'-ACACG ACGTGGAGGGAGCACA        GTAAGAGTTCTTAATTTCAGAATGTCCG        TTTCCTGACTCCACCG TATAG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 008-126184331
Forward Primer: 5'-CTGAGTGGTGAAGAAACTTGCCC-3' Tm: 59.148
Reverse Primer: 5'-GCCAAGCTTACCTGTCTAGAACTG-3' Tm: 59.038
Expected PCR Product Length: 310
Expected CelI Cleavage Product Sizes: 150, 160
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr8:126184449-126184508

Rank: 75	Homology Score: 2	RVD Match Score: 2.85	Left Half-Site RVD Score: 5.876	Right Half-Site RVD Score: 4.504
	Genomic Region: Intron	Closest Gene: EDA
Match Type: R29R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-ATCTT GGCTCCTCCCTCATGT       TATTCTATTAAAGATAGGAAACACATTGA        ATAGTAGTCAGGTTTC CTGTC-3'
	3'-TAGAA CCGAGGAGGGAGTACA       ATAAGATAATTTCTATCCTTTGTGTAACT        TATCATCAGTCCAAAG GACAG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 00X-069048457
Forward Primer: 5'-GTGAGATGAAACCAGTGCCTCAG-3' Tm: 59.148
Reverse Primer: 5'-GAACTGAAGGGTACACTAGCTGTC-3' Tm: 59.038
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 136, 134
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chrX:69048561-69048621

Rank: 76	Homology Score: 13	RVD Match Score: 2.85	Left Half-Site RVD Score: 5.333	Right Half-Site RVD Score: 5.005
	Genomic Region: Intergenic	Closest Gene: GAS2L3
Match Type: R29R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TTTTG CGCACCCGACTGCCGT       GGAGTGTTCCAGCAGAAGGTCGTCCACGT        ACAGGAGCTAGATGCA TCCTG-3'
	3'-AAAAC GCGTGGGCTGACGGCA       CCTCACAAGGTCGTCTTCCAGCAGGTGCA        TGTCCTCGATCTACGT AGGAC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 012-101038644
Forward Primer: 5'-CTGTGCTCCCCTTTCTGAATAGTG-3' Tm: 59.038
Reverse Primer: 5'-TACACTTGGACCCGACTTCCC-3' Tm: 59.378
Expected PCR Product Length: 272
Expected CelI Cleavage Product Sizes: 141, 131
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr12:101038753-101038813

Rank: 77	Homology Score: 5	RVD Match Score: 2.851	Left Half-Site RVD Score: 4.588	Right Half-Site RVD Score: 5.787
	Genomic Region: Intergenic	Closest Gene: FAM49A
Match Type: R16R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GAAAA GGCTCCTCACTCCTCT              TCTGAGGTTTACTATT              ACTGGAGTTAGGAACT TTTCT-3'
	3'-CTTTT CCGAGGAGTGAGGAGA              AGACTCCAAATGATAA              TGACCTCAATCCTTGA AAAGA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 002-016692025
Forward Primer: 5'-CTCCTGATAGTAAGGGCTGTCTC-3' Tm: 59.148
Reverse Primer: 5'-ATCAAAGCTGGCCCTTCCTGAC-3' Tm: 59.258
Expected PCR Product Length: 276
Expected CelI Cleavage Product Sizes: 146, 130
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr2:16692145-16692192

Rank: 78	Homology Score: 5	RVD Match Score: 2.851	Left Half-Site RVD Score: 6.298	Right Half-Site RVD Score: 4.154
	Genomic Region: Intergenic	Closest Gene: LINC00347
Match Type: R27R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-AAAGT AGAAGCTGGCTCCTCT        TACATGGCCAGAGCAAGAGCAAGACAG         GGAGGAGTGAGGTGCT ACACA-3'
	3'-TTTCA TCTTCGACCGAGGAGA        ATGTACCGGTCTCGTTCTCGTTCTGTC         CCTCCTCACTCCACGA TGTGT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 013-075094100
Forward Primer: 5'-ATTGGCTCACGGTTCTGTAGGC-3' Tm: 59.258
Reverse Primer: 5'-TGGATTGTGGGAGTGGATCCTTC-3' Tm: 59.148
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 135, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr13:75094204-75094262

Rank: 79	Homology Score: 2	RVD Match Score: 2.851	Left Half-Site RVD Score: 4.351	Right Half-Site RVD Score: 6.062
	Genomic Region: Intron	Closest Gene: CEP55
Match Type: R21R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-ATATA TACACCCAACTCCCAT           ATCTGGGTAATTTGGATAATT            ACAGTAGTTATCTGGA TAAAT-3'
	3'-TATAT ATGTGGGTTGAGGGTA           TAGACCCATTAAACCTATTAA            TGTCATCAATAGACCT ATTTA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 010-095287016
Forward Primer: 5'-GAGCTGCTAAAATTGCTTGCTGCC-3' Tm: 59.038
Reverse Primer: 5'-GCACCCAGCCTGTTCAGCAA-3' Tm: 59.508
Expected PCR Product Length: 335
Expected CelI Cleavage Product Sizes: 179, 156
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr10:95287167-95287219

Rank: 80	Homology Score: 5	RVD Match Score: 2.851	Left Half-Site RVD Score: 5.088	Right Half-Site RVD Score: 5.258
	Genomic Region: Intergenic	Closest Gene: DCAF5
Match Type: R27R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TTATT CACAACAGACACCTGT        TTCTCACCATTCTAGGGACTGGGAAGC         CCAGGATTAAGGTGCT GGCAG-3'
	3'-AATAA GTGTTGTCTGTGGACA        AAGAGTGGTAAGATCCCTGACCCTTCG         GGTCCTAATTCCACGA CCGTC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 014-069492113
Forward Primer: 5'-GCCTTCTCACTGAGGCCTTC-3' Tm: 59.508
Reverse Primer: 5'-TCCCAGAGAGCTTTCTTGCTCC-3' Tm: 59.258
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 135, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr14:69492217-69492275

Rank: 81	Homology Score: 5	RVD Match Score: 2.852	Left Half-Site RVD Score: 4.985	Right Half-Site RVD Score: 5.366
	Genomic Region: Intergenic	Closest Gene: LINC00290
Match Type: R25R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CTTTA AACATCTGACTCCACT         CTCTTCTTGTTTGCAGGATTTCTGA          AGAGAAGTCAGATGTA ATACT-3'
	3'-GAAAT TTGTAGACTGAGGTGA         GAGAAGAACAAACGTCCTAAAGACT          TCTCTTCAGTCTACAT TATGA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 004-180706838
Forward Primer: 5'-GCACAGTGGTTCTATTTGCAACACATTTCC-3' Tm: 60.058
Reverse Primer: 5'-CAAACCACAGACCCAGAAACCTC-3' Tm: 59.148
Expected PCR Product Length: 374
Expected CelI Cleavage Product Sizes: 168, 206
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr4:180706976-180707032

Rank: 82	Homology Score: 13	RVD Match Score: 2.856	Left Half-Site RVD Score: 5.066	Right Half-Site RVD Score: 5.318
	Genomic Region: Intron	Closest Gene: GRIA2
Match Type: R19R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-ATCCA TACACCTGTCTGCTGT            AACCTTTTCCAGATTGGCT             ACTGGAGTGAAGTGGA CAAAA-3'
	3'-TAGGT ATGTGGACAGACGACA            TTGGAAAAGGTCTAACCGA             TGACCTCACTTCACCT GTTTT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
No Primers
Primer Index: No Primers
Forward Primer: 5'-No Primers-3' Tm: No Primers
Reverse Primer: 5'-No Primers-3' Tm: No Primers
Expected PCR Product Length: No Primers
Expected CelI Cleavage Product Sizes: No Primers, No Primers
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr4:158255134-158255184

Rank: 83	Homology Score: 8	RVD Match Score: 2.857	Left Half-Site RVD Score: 4.408	Right Half-Site RVD Score: 6.04
	Genomic Region: Intron	Closest Gene: HOMER1
Match Type: R15R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-AAGGA TACCCCTAAATCCTGT              CATTCTCCCACTTAA               CCTGGATTCAGGTGAA GACCT-3'
	3'-TTCCT ATGGGGATTTAGGACA              GTAAGAGGGTGAATT               GGACCTAAGTCCACTT CTGGA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 005-078702362
Forward Primer: 5'-GAAAGGATCCTGGATGAGGGG-3' Tm: 59.378
Reverse Primer: 5'-GTAGGAGTTTCCTGCCTCTGTC-3' Tm: 59.258
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 132, 138
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr5:78702469-78702515

Rank: 84	Homology Score: 5	RVD Match Score: 2.858	Left Half-Site RVD Score: 4.624	Right Half-Site RVD Score: 5.804
	Genomic Region: Intergenic	Closest Gene: PCDH1
Match Type: R17R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GATAT AACTCCTGACTCCTGA             TCGCCTTTGTCTGTGGA              AAAGGAGTCAGGCCTC TCTCC-3'
	3'-CTATA TTGAGGACTGAGGACT             AGCGGAAACAGACACCT              TTTCCTCAGTCCGGAG AGAGG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 005-141162758
Forward Primer: 5'-GTGCTTAGGAGGCCATGGTG-3' Tm: 59.508
Reverse Primer: 5'-GCGAAGACTCAGTAAGTGGTGAC-3' Tm: 59.148
Expected PCR Product Length: 300
Expected CelI Cleavage Product Sizes: 146, 154
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr5:141162878-141162926

Rank: 85	Homology Score: 5	RVD Match Score: 2.86	Left Half-Site RVD Score: 5.088	Right Half-Site RVD Score: 5.325
	Genomic Region: Intron	Closest Gene: CLDN18
Match Type: R29R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GGGAT AGCACCTGAAACCTAA       GGAAGGAATGTATGTGGACCATAGGAAAA        GCTGGAGTCAGGAGCT CAAAC-3'
	3'-CCCTA TCGTGGACTTTGGATT       CCTTCCTTACATACACCTGGTATCCTTTT        CGACCTCAGTCCTCGA GTTTG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 003-137740660
Forward Primer: 5'-GAGGGGTGGAAACTCTCCAAAAAC-3' Tm: 59.038
Reverse Primer: 5'-TGCTGTCCTTGGGAGTCCGT-3' Tm: 59.508
Expected PCR Product Length: 338
Expected CelI Cleavage Product Sizes: 207, 131
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr3:137740835-137740895

Rank: 86	Homology Score: 5	RVD Match Score: 2.861	Left Half-Site RVD Score: 3.938	Right Half-Site RVD Score: 6.651
	Genomic Region: Exon	Closest Gene: FAM198B
Match Type: R22R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-AGGCA AACACCTCACTCATGT           CCAAGGGCTGCTTGAGAAGCCC           ACAGGGGCTAGGGCCA CAGCG-3'
	3'-TCCGT TTGTGGAGTGAGTACA           GGTTCCCGACGAACTCTTCGGG           TGTCCCCGATCCCGGT GTCGC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 004-159091578
Forward Primer: 5'-CCCCAACAGCTGCAAATAACGAAG-3' Tm: 59.038
Reverse Primer: 5'-ATGCGACTCTTGGCGGACAG-3' Tm: 59.508
Expected PCR Product Length: 281
Expected CelI Cleavage Product Sizes: 146, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr4:159091695-159091748

Rank: 87	Homology Score: 13	RVD Match Score: 2.861	Left Half-Site RVD Score: 5.028	Right Half-Site RVD Score: 5.394
	Genomic Region: Intron	Closest Gene: C14orf166B
Match Type: R24R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TAACT TGCCCCCGACTCATGT          TTTCTTAGATTATCTCTGGCAATC          ACAGGAGGCAGAGGTA AAGCT-3'
	3'-ATTGA ACGGGGGCTGAGTACA          AAAGAATCTAATAGAGACCGTTAG          TGTCCTCCGTCTCCAT TTCGA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 014-077327606
Forward Primer: 5'-GCCTGGTATAGAACAAGCACCTG-3' Tm: 59.148
Reverse Primer: 5'-CCTCCTGTGATTGCCAGAGC-3' Tm: 59.508
Expected PCR Product Length: 368
Expected CelI Cleavage Product Sizes: 179, 189
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr14:77327755-77327810

Rank: 88	Homology Score: 5	RVD Match Score: 2.862	Left Half-Site RVD Score: 5.421	Right Half-Site RVD Score: 5.005
	Genomic Region: Intron	Closest Gene: POLK
Match Type: R12R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GCCTG GGCAACCCACCCCTGT                CTCTAAAAAACA                ACAAGAGCCAGGTGTG GTGGC-3'
	3'-CGGAC CCGTTGGGTGGGGACA                GAGATTTTTTGT                TGTTCTCGGTCCACAC CACCG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 005-074840050
Forward Primer: 5'-TTTAAAGGGCAAGGCAGCCAGG-3' Tm: 59.258
Reverse Primer: 5'-GCACACCACCATGCCTGGTT-3' Tm: 59.508
Expected PCR Product Length: 290
Expected CelI Cleavage Product Sizes: 131, 159
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr5:74840157-74840200

Rank: 89	Homology Score: 2	RVD Match Score: 2.863	Left Half-Site RVD Score: 5.491	Right Half-Site RVD Score: 4.947
	Genomic Region: Intron	Closest Gene: ADCY8
Match Type: R14R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TACTG AATCCCTGAATCCTGT               GCTCCAGATGTGTT               CTAAGAGTCAGGTGTG AACCT-3'
	3'-ATGAC TTAGGGACTTAGGACA               CGAGGTCTACACAA               GATTCTCAGTCCACAC TTGGA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 008-132042625
Forward Primer: 5'-GAGGAAGATCCATACCCTTCCAC-3' Tm: 59.148
Reverse Primer: 5'-GCCTGCTGGGTTTTATGTCCTAG-3' Tm: 59.148
Expected PCR Product Length: 333
Expected CelI Cleavage Product Sizes: 180, 153
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr8:132042780-132042825

Rank: 90	Homology Score: 10	RVD Match Score: 2.864	Left Half-Site RVD Score: 4.639	Right Half-Site RVD Score: 5.831
	Genomic Region: Intron	Closest Gene: JAKMIP3
Match Type: R16R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-AGGGG TGCACCTCACAACTGT              GGGAGCAGGTGATGTG              GCATGAGGCAGGGGCG GGGCT-3'
	3'-TCCCC ACGTGGAGTGTTGACA              CCCTCGTCCACTACAC              CGTACTCCGTCCCCGC CCCGA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 010-133959783
Forward Primer: 5'-TTTCTCTCGGCACCTCCCAC-3' Tm: 59.508
Reverse Primer: 5'-GTCCCTGCCCATGGACAAGT-3' Tm: 59.508
Expected PCR Product Length: 287
Expected CelI Cleavage Product Sizes: 157, 130
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr10:133959914-133959961

Rank: 91	Homology Score: 10	RVD Match Score: 2.864	Left Half-Site RVD Score: 6.435	Right Half-Site RVD Score: 4.129
	Genomic Region: Intergenic	Closest Gene: SALL3
Match Type: R14R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GCCCG GGCACTTGACCCTTGA               CCAGTGGCCACCAC               TCAGGAGGCAGGTGCC ACCAC-3'
	3'-CGGGC CCGTGAACTGGGAACT               GGTCACCGGTGGTG               AGTCCTCCGTCCACGG TGGTG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 018-076525272
Forward Primer: 5'-TGGTGTCTCCCGTGTGGCTT-3' Tm: 59.508
Reverse Primer: 5'-CACTTTCCCAAACGTCACGGC-3' Tm: 59.378
Expected PCR Product Length: 312
Expected CelI Cleavage Product Sizes: 131, 181
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr18:76525378-76525423

Rank: 92	Homology Score: 10	RVD Match Score: 2.866	Left Half-Site RVD Score: 6.424	Right Half-Site RVD Score: 4.15
	Genomic Region: Intron	Closest Gene: MLXIPL
Match Type: R17R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CACCC TTCACCCCATCCCTGT             CTGCTCAGCGCAAGGCT              ACAGGAGGCAGGGGCT GGTCT-3'
	3'-GTGGG AAGTGGGGTAGGGACA             GACGAGTCGCGTTCCGA              TGTCCTCCGTCCCCGA CCAGA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 007-073021712
Forward Primer: 5'-GATGCGCCGCTTCCAGTAGT-3' Tm: 59.508
Reverse Primer: 5'-TGTGAAGCGGAGGAAGAGCC-3' Tm: 59.508
Expected PCR Product Length: 290
Expected CelI Cleavage Product Sizes: 130, 160
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr7:73021816-73021864

Rank: 93	Homology Score: 10	RVD Match Score: 2.867	Left Half-Site RVD Score: 5.747	Right Half-Site RVD Score: 4.74
	Genomic Region: Intergenic	Closest Gene: TNFSF15
Match Type: R20R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-ATCAC CCCAGCAGACCCCTGT            GGGTTTGCTCACATAAGGGA            ACAGGAGTTATGAGCA ATGAA-3'
	3'-TAGTG GGGTCGTCTGGGGACA            CCCAAACGAGTGTATTCCCT            TGTCCTCAATACTCGT TACTT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 009-117519299
Forward Primer: 5'-CAAAAGGACCTGCATCTAAGCACC-3' Tm: 59.038
Reverse Primer: 5'-ATTGTTCTTCCCTGGCCACCC-3' Tm: 59.378
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 135, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr9:117519406-117519457

Rank: 94	Homology Score: 2	RVD Match Score: 2.867	Left Half-Site RVD Score: 4.769	Right Half-Site RVD Score: 5.716
	Genomic Region: Intergenic	Closest Gene: SLC44A1
Match Type: R18R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TGGAT GGCAACTAACTTCTAT             ACAACATCTTGCTTCCAG             CCAGGAGTCTGGTTGG AAGAG-3'
	3'-ACCTA CCGTTGATTGAAGATA             TGTTGTAGAACGAAGGTC             GGTCCTCAGACCAACC TTCTC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 009-107888167
Forward Primer: 5'-CACTTCTAGGACTCAGTCGTCATG-3' Tm: 59.038
Reverse Primer: 5'-GTGGGCATGGTAAGAGGTATTGCT-3' Tm: 59.038
Expected PCR Product Length: 320
Expected CelI Cleavage Product Sizes: 167, 153
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr9:107888307-107888356

Rank: 95	Homology Score: 5	RVD Match Score: 2.868	Left Half-Site RVD Score: 6.083	Right Half-Site RVD Score: 6.405
	Genomic Region: Intergenic	Closest Gene: LYSMD4
Match Type: R30L
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GAGCG TGCCCCTGGCTCCCTT       CTGCCTCCTGACCAACACTGGTGCATCCCT ATGGCCCTGTGGGGCCTGGTGT GCTCC-3'
	3'-CTCGC ACGGGGACCGAGGGAA       GACGGAGGACTGGTTGTGACCACGTAGGGA TACCGGGACACCCCGGACCACA CGAGG-5'
	                                                               TGACGGGACACCCCGTTCCACT
	                                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 015-100263695
Forward Primer: 5'-GACTGAGGAGAGACTGCTGTTC-3' Tm: 59.258
Reverse Primer: 5'-GTGGAGACCTTAAGAGATGTCTCG-3' Tm: 59.038
Expected PCR Product Length: 304
Expected CelI Cleavage Product Sizes: 131, 173
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr15:100263790-100263857

Rank: 96	Homology Score: 2	RVD Match Score: 2.87	Left Half-Site RVD Score: 5.009	Right Half-Site RVD Score: 5.479
	Genomic Region: Intergenic	Closest Gene: MGC27382
Match Type: R10R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CTTCT CCCAACTTACTCCTCT                 CGTTCCAACA                 AAAGGAGCTATGTGCT CTTCC-3'
	3'-GAAGA GGGTTGAATGAGGAGA                 GCAAGGTTGT                 TTTCCTCGATACACGA GAAGG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 001-078685858
Forward Primer: 5'-GCTGGTCTGCCACAGTGCTT-3' Tm: 59.508
Reverse Primer: 5'-CCTGCACAAATGTCTCTTGGAACC-3' Tm: 59.038
Expected PCR Product Length: 304
Expected CelI Cleavage Product Sizes: 154, 150
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr1:78685989-78686030

Rank: 97	Homology Score: 2	RVD Match Score: 2.87	Left Half-Site RVD Score: 5.377	Right Half-Site RVD Score: 5.109
	Genomic Region: Intergenic	Closest Gene: DANCR
Match Type: R12R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TCCAA GTCACATGACACCTCT                GGAGCCGCCAAT                ACATGAGGTGGGTGCT GCCAG-3'
	3'-AGGTT CAGTGTACTGTGGAGA                CCTCGGCGGTTA                TGTACTCCACCCACGA CGGTC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 004-053572737
Forward Primer: 5'-CTAACAATGTGTCCATGGGGTTGC-3' Tm: 59.038
Reverse Primer: 5'-GGGATTACAGGCATGAGCCAC-3' Tm: 59.378
Expected PCR Product Length: 286
Expected CelI Cleavage Product Sizes: 150, 136
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr4:53572863-53572906

Rank: 98	Homology Score: 10	RVD Match Score: 2.871	Left Half-Site RVD Score: 7.308	Right Half-Site RVD Score: 3.524
	Genomic Region: Intron	Closest Gene: ZNF676
Match Type: R15R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CCTAC TGCCGCTGTCTCCTTC              TTATTTTGTCAGACA               ACAGGAGTCAGGTTTT GAGTC-3'
	3'-GGATG ACGGCGACAGAGGAAG              AATAAAACAGTCTGT               TGTCCTCAGTCCAAAA CTCAG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 019-022376173
Forward Primer: 5'-GGTACTCTCACTGTGGAAAACTGG-3' Tm: 59.038
Reverse Primer: 5'-GGCCAATTCTCCTCTCCTTTTTCC-3' Tm: 59.038
Expected PCR Product Length: 287
Expected CelI Cleavage Product Sizes: 146, 141
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr19:22376294-22376340

Rank: 99	Homology Score: 2	RVD Match Score: 2.871	Left Half-Site RVD Score: 5.531	Right Half-Site RVD Score: 4.969
	Genomic Region: Intergenic	Closest Gene: GJB5
Match Type: R18R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GCAGC CACAGCAGAATCCTGT             TAGGAGGCTGTCCTGGGG             ATAGGAGGCAGGGGAT GGGAG-3'
	3'-CGTCG GTGTCGTCTTAGGACA             ATCCTCCGACAGGACCCC             TATCCTCCGTCCCCTA CCCTC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 001-035182032
Forward Primer: 5'-ATGCAGAAAATCCGGATGGCCTGG-3' Tm: 60.908
Reverse Primer: 5'-GGGACCTTGTGATAGAAGCCTG-3' Tm: 59.258
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 132, 138
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr1:35182137-35182186

Rank: 100	Homology Score: 5	RVD Match Score: 2.872	Left Half-Site RVD Score: 4.478	Right Half-Site RVD Score: 6.076
	Genomic Region: Intron	Closest Gene: MGAM
Match Type: R22R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GAGAT GTCACCTCACACCTGT           TAGCATGGAAATCATAAAAAAG           AAAGGAGAGAAGTGCT GGTGA-3'
	3'-CTCTA CAGTGGAGTGTGGACA           ATCGTACCTTTAGTATTTTTTC           TTTCCTCTCTTCACGA CCACT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 007-141775417
Forward Primer: 5'-GGGTGAAGGACCTGAACAGATG-3' Tm: 59.258
Reverse Primer: 5'-CCCAGAAGTGGGAATTCATGGGA-3' Tm: 59.148
Expected PCR Product Length: 318
Expected CelI Cleavage Product Sizes: 150, 168
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr7:141775538-141775591

Rank: 101	Homology Score: 2	RVD Match Score: 2.873	Left Half-Site RVD Score: 6.197	Right Half-Site RVD Score: 4.387
	Genomic Region: Intergenic	Closest Gene: PTPRK
Match Type: R14R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TCCTA TGTACCTAATTCTTAT               CTCTAAAAATTTTT               ATTTGAGTCAGGTGTC AAAAT-3'
	3'-AGGAT ACATGGATTAAGAATA               GAGATTTTTAAAAA               TAAACTCAGTCCACAG TTTTA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 006-128845862
Forward Primer: 5'-GAAGGAGGCAAGGCAGGTATG-3' Tm: 59.378
Reverse Primer: 5'-CGTACCCATGCAAGAAGTTTCCTG-3' Tm: 59.038
Expected PCR Product Length: 336
Expected CelI Cleavage Product Sizes: 189, 147
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr6:128846026-128846071

Rank: 102	Homology Score: 5	RVD Match Score: 2.874	Left Half-Site RVD Score: 6.299	Right Half-Site RVD Score: 4.306
	Genomic Region: Intron	Closest Gene: CUX1
Match Type: R28R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TGTCC TCCCCCTCACTGATGT        TTTCTGCTCATTGGGTGGGGGAGGAATC        ACATGGGTTAGGTGCC CATCC-3'
	3'-ACAGG AGGGGGAGTGACTACA        AAAGACGAGTAACCCACCCCCTCCTTAG        TGTACCCAATCCACGG GTAGG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 007-101606440
Forward Primer: 5'-CTCACCTGGCAGGAAAATTCCC-3' Tm: 59.258
Reverse Primer: 5'-ATGAACTGCTGGGTGCAGCC-3' Tm: 59.508
Expected PCR Product Length: 278
Expected CelI Cleavage Product Sizes: 133, 145
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr7:101606541-101606600

Rank: 103	Homology Score: 5	RVD Match Score: 2.876	Left Half-Site RVD Score: 4.154	Right Half-Site RVD Score: 6.502
	Genomic Region: Intergenic	Closest Gene: PALD1
Match Type: R20R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TGGAA ACCACCTCACACCTGT            TAGGATGGCTGCAGGAAGAG            ACAGAAGAGAAGTGCT GGCGA-3'
	3'-ACCTT TGGTGGAGTGTGGACA            ATCCTACCGACGTCCTTCTC            TGTCTTCTCTTCACGA CCGCT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 010-072329199
Forward Primer: 5'-CGGCATTTGCAAACCATCCATCTG-3' Tm: 59.038
Reverse Primer: 5'-TTTTGGTTTTCTGAGGACCCCCC-3' Tm: 59.148
Expected PCR Product Length: 327
Expected CelI Cleavage Product Sizes: 197, 130
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr10:72329368-72329419

Rank: 104	Homology Score: 2	RVD Match Score: 2.876	Left Half-Site RVD Score: 4.892	Right Half-Site RVD Score: 5.648
	Genomic Region: Intron	Closest Gene: RASSF4
Match Type: R10R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TCAGC GTCACCACACTCCTCT                 GGGGCTGCCT                 GTAGGAATCCGGTGCC CTGAG-3'
	3'-AGTCG CAGTGGTGTGAGGAGA                 CCCCGACGGA                 CATCCTTAGGCCACGG GACTC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 010-045473446
Forward Primer: 5'-ATGGGCCACGGAGAGCAGAA-3' Tm: 59.508
Reverse Primer: 5'-GCCTTCCTCTGACCAGTGTTTG-3' Tm: 59.258
Expected PCR Product Length: 326
Expected CelI Cleavage Product Sizes: 140, 186
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr10:45473563-45473604

Rank: 105	Homology Score: 10	RVD Match Score: 2.876	Left Half-Site RVD Score: 5.619	Right Half-Site RVD Score: 4.921
	Genomic Region: Intron	Closest Gene: MED27
Match Type: R27R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TTGTT TACAAATCACTCCTGC        CATCAACCTCATTTTACAGATCAGGAA         ACAGGAGTGAGCTGAA CAACG-3'
	3'-AACAA ATGTTTAGTGAGGACG        GTAGTTGGAGTAAAATGTCTAGTCCTT         TGTCCTCACTCGACTT GTTGC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 009-134936820
Forward Primer: 5'-GCTGGTAAGTGAAGTTGATGTGGC-3' Tm: 59.038
Reverse Primer: 5'-CCAGGAGCAGCTGCGATGAA-3' Tm: 59.508
Expected PCR Product Length: 318
Expected CelI Cleavage Product Sizes: 187, 131
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr9:134936976-134937034

Rank: 106	Homology Score: 2	RVD Match Score: 2.877	Left Half-Site RVD Score: 5.325	Right Half-Site RVD Score: 5.205
	Genomic Region: Intron	Closest Gene: AOC2
Match Type: R22R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-ACCCT GCCACCAGCCTCCTGA           GTTGCAAGCTGCCACACCCGGC           TCAGGAGTCAGTTTTT CAGCT-3'
	3'-TGGGA CGGTGGTCGGAGGACT           CAACGTTCGACGGTGTGGGCCG           AGTCCTCAGTCAAAAA GTCGA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 017-041000067
Forward Primer: 5'-GCTACCATGCCCGGCCTTTT-3' Tm: 59.508
Reverse Primer: 5'-GCTGTTCTATGGCTGTGCCC-3' Tm: 59.508
Expected PCR Product Length: 274
Expected CelI Cleavage Product Sizes: 142, 132
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr17:41000180-41000233

Rank: 107	Homology Score: 5	RVD Match Score: 2.877	Left Half-Site RVD Score: 5.205	Right Half-Site RVD Score: 5.325
	Genomic Region: Intergenic	Closest Gene: LSAMP-AS3
Match Type: R12R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-AGTTT TGCACCAAAATACTGT                GAAAGAGAAGAA                AGTGGTGGCAGGTGCT GGTGG-3'
	3'-TCAAA ACGTGGTTTTATGACA                CTTTCTCTTCTT                TCACCACCGTCCACGA CCACC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 003-116802161
Forward Primer: 5'-GGGATGGTCCAAAACTCAAACCTC-3' Tm: 59.038
Reverse Primer: 5'-GAAGGCATTGGGGCTAGACAAG-3' Tm: 59.258
Expected PCR Product Length: 362
Expected CelI Cleavage Product Sizes: 177, 185
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr3:116802314-116802357

Rank: 108	Homology Score: 2	RVD Match Score: 2.878	Left Half-Site RVD Score: 5.849	Right Half-Site RVD Score: 4.72
	Genomic Region: Intergenic	Closest Gene: C17orf66
Match Type: R27R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CTTTA TGCCCCTCACTGCCAT        CTTGTCTTCTGGTGGGGACTGGTGAAA         TTAGGATTGAGGTGCT ACTAG-3'
	3'-GAAAT ACGGGGAGTGACGGTA        GAACAGAAGACCACCCCTGACCACTTT         AATCCTAACTCCACGA TGATC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 017-034179828
Forward Primer: 5'-CCAGCGACTGCTTGGATAAAGTAG-3' Tm: 59.038
Reverse Primer: 5'-CTGCAAAATCCTGCCCACAGATC-3' Tm: 59.148
Expected PCR Product Length: 282
Expected CelI Cleavage Product Sizes: 151, 131
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr17:34179948-34180006

Rank: 109	Homology Score: 5	RVD Match Score: 2.879	Left Half-Site RVD Score: 5.318	Right Half-Site RVD Score: 5.228
	Genomic Region: Intergenic	Closest Gene: SNX11
Match Type: R26R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TAAGA GGCATCCCACTCCTCT         CCTTGAGTAATGAGGGCTGGGAGCAC         AAGGGAGTCAGATGCT GATTA-3'
	3'-ATTCT CCGTAGGGTGAGGAGA         GGAACTCATTACTCCCGACCCTCGTG         TTCCCTCAGTCTACGA CTAAT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 017-046203475
Forward Primer: 5'-GCAATGCAGCCTGCCTGCTA-3' Tm: 59.508
Reverse Primer: 5'-CTGAGGCACCTGGAAGGGTT-3' Tm: 59.508
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 133, 137
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr17:46203577-46203634

Rank: 110	Homology Score: 2	RVD Match Score: 2.88	Left Half-Site RVD Score: 4.705	Right Half-Site RVD Score: 5.884
	Genomic Region: Intergenic	Closest Gene: NOVA1
Match Type: R17R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TTCCA AACTCCTAACTCCTGA             TTTAGCACCAGGAAGGT              TTAGGATTGAAGTGCA ATTAA-3'
	3'-AAGGT TTGAGGATTGAGGACT             AAATCGTGGTCCTTCCA              AATCCTAACTTCACGT TAATT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 014-026548055
Forward Primer: 5'-GGGAGGCTGACTTGAATGAGCT-3' Tm: 59.258
Reverse Primer: 5'-GTTGCCAGGTGAATAAAAGGAGAGTGAC-3' Tm: 60.298
Expected PCR Product Length: 426
Expected CelI Cleavage Product Sizes: 138, 288
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr14:26548167-26548215

Rank: 111	Homology Score: 5	RVD Match Score: 2.881	Left Half-Site RVD Score: 6.075	Right Half-Site RVD Score: 4.543
	Genomic Region: Intergenic	Closest Gene: MOSPD1
Match Type: R24R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-AGCCT GGCCTCTGACACCTGC          TAATCTGTGCCTTGTTGTCTGTCC          TCAGGAGGCAGGTGGC TTCCT-3'
	3'-TCGGA CCGGAGACTGTGGACG          ATTAGACACGGAACAACAGACAGG          AGTCCTCCGTCCACCG AAGGA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 00X-134006532
Forward Primer: 5'-CGCAAAGGTCATCAGTAAGCACAC-3' Tm: 59.038
Reverse Primer: 5'-GAAAGAGGAGGCTTAGAGTTGGG-3' Tm: 59.148
Expected PCR Product Length: 274
Expected CelI Cleavage Product Sizes: 138, 136
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chrX:134006640-134006695

Rank: 112	Homology Score: 5	RVD Match Score: 2.882	Left Half-Site RVD Score: 5.47	Right Half-Site RVD Score: 5.102
	Genomic Region: Intergenic	Closest Gene: HSP90AB2P
Match Type: R26R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-ATTGG GGCACTTGAATCCAAT         AGAAGCATGACAGATGACAACCAGGC         AGAGTAGTTAAGTGCA TTCTC-3'
	3'-TAACC CCGTGAACTTAGGTTA         TCTTCGTACTGTCTACTGTTGGTCCG         TCTCATCAATTCACGT AAGAG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 004-012977409
Forward Primer: 5'-GACACTTGAAGCCTGAGAATCGC-3' Tm: 59.148
Reverse Primer: 5'-CAACTGTGAGCCTGTGTAGATGG-3' Tm: 59.148
Expected PCR Product Length: 369
Expected CelI Cleavage Product Sizes: 200, 169
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr4:12977578-12977635

Rank: 113	Homology Score: 2	RVD Match Score: 2.882	Left Half-Site RVD Score: 5.701	Right Half-Site RVD Score: 4.883
	Genomic Region: Intron	Closest Gene: DLGAP5
Match Type: R16R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CATCT AGCTCCTAACGCCTGC              TATATGAACACAACAA              ATAGGAGTAGGGTGAC TCCTG-3'
	3'-GTAGA TCGAGGATTGCGGACG              ATATACTTGTGTTGTT              TATCCTCATCCCACTG AGGAC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 014-055654691
Forward Primer: 5'-CCTCTAAGCCTTTGCAAACGGG-3' Tm: 59.258
Reverse Primer: 5'-GGGACCATTAGGATGGAAAGAACC-3' Tm: 59.038
Expected PCR Product Length: 388
Expected CelI Cleavage Product Sizes: 227, 161
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr14:55654892-55654939

Rank: 114	Homology Score: 5	RVD Match Score: 2.883	Left Half-Site RVD Score: 5.796	Right Half-Site RVD Score: 4.802
	Genomic Region: Intron	Closest Gene: ATP10A
Match Type: R29R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-AGTTT AACAGCTGACTTCTGA       TCAGAAATCATGAAGGACGTGAAGGACAA        AATGGAGTCAGGTGAC ATCTT-3'
	3'-TCAAA TTGTCGACTGAAGACT       AGTCTTTAGTACTTCCTGCACTTCCTGTT        TTACCTCAGTCCACTG TAGAA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 015-026072103
Forward Primer: 5'-GTCAAAAGCCCAAAACCCTACATTCGG-3' Tm: 60.438
Reverse Primer: 5'-GTCTGCTAGTGGTGACTTCTCTG-3' Tm: 59.148
Expected PCR Product Length: 289
Expected CelI Cleavage Product Sizes: 133, 156
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr15:26072204-26072264

Rank: 115	Homology Score: 13	RVD Match Score: 2.883	Left Half-Site RVD Score: 6.616	Right Half-Site RVD Score: 4.108
	Genomic Region: Exon	Closest Gene: CD300LD
Match Type: R16R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CCACA TGGTCCTGTCTCCTCT              CCTCTCCTTGGTGATC              ACAGGTGTCTGGTGCC CTTCA-3'
	3'-GGTGT ACCAGGACAGAGGAGA              GGAGAGGAACCACTAG              TGTCCACAGACCACGG GAAGT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 017-072588236
Forward Primer: 5'-CAGAGCGTCTCTGTCTCCAAG-3' Tm: 59.378
Reverse Primer: 5'-GGCACACCTTGCAACATACACTG-3' Tm: 59.148
Expected PCR Product Length: 329
Expected CelI Cleavage Product Sizes: 131, 198
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr17:72588341-72588388

Rank: 116	Homology Score: 5	RVD Match Score: 2.886	Left Half-Site RVD Score: 6.121	Right Half-Site RVD Score: 4.536
	Genomic Region: Intron	Closest Gene: VASH2
Match Type: R10R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GGTGG TCCACCTGCCCCTTCT                 GGTATTTTAT                 AGAGGAGTCAGATGGG AAGCT-3'
	3'-CCACC AGGTGGACGGGGAAGA                 CCATAAAATA                 TCTCCTCAGTCTACCC TTCGA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 001-213135498
Forward Primer: 5'-TGGTTTGGTCCCTTTCTTCTCCG-3' Tm: 59.148
Reverse Primer: 5'-AAGCCAGTCAATGTGCCTGGG-3' Tm: 59.378
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 135, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr1:213135610-213135651

Rank: 117	Homology Score: 2	RVD Match Score: 2.886	Left Half-Site RVD Score: 5.281	Right Half-Site RVD Score: 5.318
	Genomic Region: Intergenic	Closest Gene: FHL5
Match Type: R27R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CTGTG CTCCCCTGACTCCCCT        GCTCTATAGGTAAGGACACTGCAGCTC         ACAGAGGTGAGGTGGT CTGCT-3'
	3'-GACAC GAGGGGACTGAGGGGA        CGAGATATCCATTCCTGTGACGTCGAG         TGTCTCCACTCCACCA GACGA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 006-097126340
Forward Primer: 5'-CAGGTGAGGAAACTGAGGCATAC-3' Tm: 59.148
Reverse Primer: 5'-ATGCGAGGCAGATGAAGAGAGAC-3' Tm: 59.148
Expected PCR Product Length: 305
Expected CelI Cleavage Product Sizes: 174, 131
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr6:97126483-97126541

Rank: 118	Homology Score: 5	RVD Match Score: 2.886	Left Half-Site RVD Score: 4.617	Right Half-Site RVD Score: 6.029
	Genomic Region: Intron	Closest Gene: REV3L
Match Type: R25R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CTTAA AACACTTCACTCCTCT         CGCTTCTTGCTTGCATTGTTTGTGA          ACAGAAGTCCGATGTA ATTTT-3'
	3'-GAATT TTGTGAAGTGAGGAGA         GCGAAGAACGAACGTAACAAACACT          TGTCTTCAGGCTACAT TAAAA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Warning! Primer pair was generated using suboptimal parameters. 
Primer Index: 006-111761973
Forward Primer: 5'-GTAACATTTCTTACTGGCAACAGATTCCCTC-3' Tm: 59.958
Reverse Primer: 5'-GAGGCATAGGGATTGGAATTGTAAGAAAAGG-3' Tm: 59.958
Expected PCR Product Length: 447
Expected CelI Cleavage Product Sizes: 166, 281
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr6:111762109-111762165

Rank: 119	Homology Score: 5	RVD Match Score: 2.888	Left Half-Site RVD Score: 4.74	Right Half-Site RVD Score: 5.904
	Genomic Region: Intron	Closest Gene: AKD1
Match Type: R22R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TAAGT CACGCCTGACTCATGT           TCAAATGGAATGAATTACAGTA           ACATGAGTCAGGCATG TTTAC-3'
	3'-ATTCA GTGCGGACTGAGTACA           AGTTTACCTTACTTAATGTCAT           TGTACTCAGTCCGTAC AAATG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 006-109827303
Forward Primer: 5'-CTGGGGATTCGGTTTCAGCATATG-3' Tm: 59.038
Reverse Primer: 5'-CAGCAAATGGTGGGTATGGAATGAAGTC-3' Tm: 60.298
Expected PCR Product Length: 316
Expected CelI Cleavage Product Sizes: 152, 164
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr6:109827426-109827479

Rank: 120	Homology Score: 5	RVD Match Score: 2.891	Left Half-Site RVD Score: 4.911	Right Half-Site RVD Score: 5.739
	Genomic Region: Intron	Closest Gene: DHRS11
Match Type: R30R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TCAAT GGCACCTGCCACCTGA       ATTGCCTCATAGTCTCCTGAGAAACCTCAG       AGGGGAGGGTGGTGCA CCAGG-3'
	3'-AGTTA CCGTGGACGGTGGACT       TAACGGAGTATCAGAGGACTCTTTGGAGTC       TCCCCTCCCACCACGT GGTCC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 017-034950345
Forward Primer: 5'-GAATGTCAGCTTCCCCCTGG-3' Tm: 59.508
Reverse Primer: 5'-GCTCAGAGTTCTCAGACATGCC-3' Tm: 59.258
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 131, 139
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr17:34950443-34950504

Rank: 121	Homology Score: 2	RVD Match Score: 2.891	Left Half-Site RVD Score: 5.226	Right Half-Site RVD Score: 5.414
	Genomic Region: Intron	Closest Gene: LOC100233156
Match Type: R17R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TGGGG CAAAACTGCCTCCTGT             CACTAAGGGGCCAGATT              TCGGGACTCAGGTGTC TCACG-3'
	3'-ACCCC GTTTTGACGGAGGACA             GTGATTCCCCGGTCTAA              AGCCCTGAGTCCACAG AGTGC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 218-000043242
Forward Primer: 5'-CAATCAGGGAGGGTCTCTGG-3' Tm: 59.508
Reverse Primer: 5'-CTTGGGTGGCCAGGACTTGT-3' Tm: 59.508
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 138, 132
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chrUn_gl000218:43354-43402

Rank: 122	Homology Score: 5	RVD Match Score: 2.892	Left Half-Site RVD Score: 5.648	Right Half-Site RVD Score: 5.006
	Genomic Region: Intron	Closest Gene: ABCB9
Match Type: R19R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CCACC GATACCCGACTCCTGG            GACCCACCCCTGCTACTAG             AGAGGAGGCAGGTATA GGGAA-3'
	3'-GGTGG CTATGGGCTGAGGACC            CTGGGTGGGGACGATGATC             TCTCCTCCGTCCATAT CCCTT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 012-123425468
Forward Primer: 5'-CCGGTCGATGAACTCGAACAC-3' Tm: 59.378
Reverse Primer: 5'-AGAGTGCTAGTGATGCTGCTGTC-3' Tm: 59.148
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 135, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr12:123425576-123425626

Rank: 123	Homology Score: 5	RVD Match Score: 2.893	Left Half-Site RVD Score: 4.948	Right Half-Site RVD Score: 5.716
	Genomic Region: Intergenic	Closest Gene: CYP26B1
Match Type: R19R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GGAGC TGCCCCTAACTCCTTC            CCCCCAAGTCCTGCCCCCA             GCAGGAGTCCTGTGGG TCACT-3'
	3'-CCTCG ACGGGGATTGAGGAAG            GGGGGTTCAGGACGGGGGT             CGTCCTCAGGACACCC AGTGA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 002-072161892
Forward Primer: 5'-GGCTGCTGTCTTTGATGATGCAAC-3' Tm: 59.038
Reverse Primer: 5'-GCCCCTGGCAATGAAGGCAA-3' Tm: 59.508
Expected PCR Product Length: 278
Expected CelI Cleavage Product Sizes: 147, 131
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr2:72162012-72162062

Rank: 124	Homology Score: 2	RVD Match Score: 2.894	Left Half-Site RVD Score: 5.743	Right Half-Site RVD Score: 4.927
	Genomic Region: Intron	Closest Gene: COBL
Match Type: R19R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TTGAG GTTACCTGGCTCCTAT            GCACTAAATGTGATTCGCT             GCAGGAGTTTGGTGAG ATGCA-3'
	3'-AACTC CAATGGACCGAGGATA            CGTGATTTACACTAAGCGA             CGTCCTCAAACCACTC TACGT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 007-051111717
Forward Primer: 5'-GCTGGCCTTTGGCTTAATGGAAC-3' Tm: 59.148
Reverse Primer: 5'-CTATCTACAAATAGCTAGGTAGTTGCGCAG-3' Tm: 60.058
Expected PCR Product Length: 383
Expected CelI Cleavage Product Sizes: 250, 133
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr7:51111940-51111990

Rank: 125	Homology Score: 5	RVD Match Score: 2.894	Left Half-Site RVD Score: 4.116	Right Half-Site RVD Score: 6.695
	Genomic Region: Intron	Closest Gene: C7orf69
Match Type: R26R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CATCT CACACCTCACTCCTGG         CACACCCAAAGTGCAGGTAGTGAACC         AAATGAGCCAGGAGTA TTCTG-3'
	3'-GTAGA GTGTGGAGTGAGGACC         GTGTGGGTTTCACGTCCATCACTTGG         TTTACTCGGTCCTCAT AAGAC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 007-047858063
Forward Primer: 5'-CCACTTTCGGGGAAATGGTATCAC-3' Tm: 59.038
Reverse Primer: 5'-GCAAGCGTTCTATCCAAGTGCC-3' Tm: 59.258
Expected PCR Product Length: 310
Expected CelI Cleavage Product Sizes: 160, 150
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr7:47858192-47858249

Rank: 126	Homology Score: 5	RVD Match Score: 2.894	Left Half-Site RVD Score: 4.957	Right Half-Site RVD Score: 5.715
	Genomic Region: Intron	Closest Gene: ITPR3
Match Type: R29R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CCCTC CCCCCCCGACTCCTCT       GTCCTGGGAAACGTGGCTTTGCCTCCCAG        ACACGTGTCAGATGCC AGCTC-3'
	3'-GGGAG GGGGGGGCTGAGGAGA       CAGGACCCTTTGCACCGAAACGGAGGGTC        TGTGCACAGTCTACGG TCGAG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 006-033661964
Forward Primer: 5'-CTGAAGGGGAGCCTGGAAAAAC-3' Tm: 59.258
Reverse Primer: 5'-TCCTTCCTTCCTTCCTCCCG-3' Tm: 59.508
Expected PCR Product Length: 393
Expected CelI Cleavage Product Sizes: 250, 143
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr6:33662182-33662242

Rank: 127	Homology Score: 13	RVD Match Score: 2.894	Left Half-Site RVD Score: 4.543	Right Half-Site RVD Score: 6.18
	Genomic Region: Intron	Closest Gene: CACNA2D3
Match Type: R11R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CAAGG TGCCCCACACTCCTGT                GGTCTGGAAGG                 AATGGAGCCATGTGCA CACTT-3'
	3'-GTTCC ACGGGGTGTGAGGACA                CCAGACCTTCC                 TTACCTCGGTACACGT GTGAA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 003-054731501
Forward Primer: 5'-CCTACAGTTTCCAAGGACAAGCAG-3' Tm: 59.038
Reverse Primer: 5'-CCCAGCAGTAGCTTTAGCATCC-3' Tm: 59.258
Expected PCR Product Length: 320
Expected CelI Cleavage Product Sizes: 160, 160
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr3:54731638-54731680

Rank: 128	Homology Score: 10	RVD Match Score: 2.895	Left Half-Site RVD Score: 5.374	Right Half-Site RVD Score: 5.289
	Genomic Region: Intergenic	Closest Gene: SLC16A7
Match Type: R15R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TCTCT CACATCTGCCTCCAGT              GTACCAGCAAAGATC               ACAGGGGTCAGGTCAA GGTGG-3'
	3'-AGAGA GTGTAGACGGAGGTCA              CATGGTCGTTTCTAG               TGTCCCCAGTCCAGTT CCACC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Warning! Primer pair was generated using suboptimal parameters. 
Primer Index: 012-059855477
Forward Primer: 5'-GAGAAAGGATTGAGTATTTTTCTCAGGACTTGG-3' Tm: 59.758
Reverse Primer: 5'-CAGAATTTCAGAGGCCCTCCATC-3' Tm: 59.148
Expected PCR Product Length: 404
Expected CelI Cleavage Product Sizes: 130, 274
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr12:59855582-59855628

Rank: 129	Homology Score: 5	RVD Match Score: 2.895	Left Half-Site RVD Score: 5.346	Right Half-Site RVD Score: 7.658
	Genomic Region: Intergenic	Closest Gene: SH3BP2
Match Type: R21L
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CCCTC TGCCCCTCCCTCCAGT           GCTGGGCTGTGTGGCCAGGGG      ACTGGACTGTGTGGCAGGATGA CTCCA-3'
	3'-GGGAG ACGGGGAGGGAGGTCA           CGACCCGACACACCGGTCCCC      TGACCTGACACACCGTCCTACT GAGGT-5'
	                                                               TGACGGGACACCCCGTTCCACT
	                                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 004-002790263
Forward Primer: 5'-ATGCAGAGATGGCCCAGAGC-3' Tm: 59.508
Reverse Primer: 5'-TGAGTAGGGCCAAGGCCACA-3' Tm: 59.508
Expected PCR Product Length: 375
Expected CelI Cleavage Product Sizes: 237, 138
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr4:2790469-2790527

Rank: 130	Homology Score: 5	RVD Match Score: 2.896	Left Half-Site RVD Score: 4.639	Right Half-Site RVD Score: 6.084
	Genomic Region: Intergenic	Closest Gene: PBX1
Match Type: R17R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GCCTT ACCACCTGAATCCTGA             GAAGAAGGCAGCTAGAC              AGAGGAATCAGCTGAT GTATG-3'
	3'-CGGAA TGGTGGACTTAGGACT             CTTCTTCCGTCGATCTG              TCTCCTTAGTCGACTA CATAC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 001-163952024
Forward Primer: 5'-GCAGCCCCTTTCAACTTAGACTAG-3' Tm: 59.038
Reverse Primer: 5'-CTAGTCCCTGCCCACATTTCC-3' Tm: 59.378
Expected PCR Product Length: 281
Expected CelI Cleavage Product Sizes: 150, 131
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr1:163952148-163952196

Rank: 131	Homology Score: 2	RVD Match Score: 2.897	Left Half-Site RVD Score: 6.386	Right Half-Site RVD Score: 4.387
	Genomic Region: Intergenic	Closest Gene: EPHA3
Match Type: R14R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-AAGCA TCCACATGTCTCATCT               CATTTGATATCCCA               ATTTGAGTCAGGTGTT ACTTC-3'
	3'-TTCGT AGGTGTACAGAGTAGA               GTAAACTATAGGGT               TAAACTCAGTCCACAA TGAAG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Warning! Primer pair was generated using suboptimal parameters. 
Primer Index: 003-089962457
Forward Primer: 5'-CCATGACATTGAGAACAGTCGTTTTCCTAG-3' Tm: 60.058
Reverse Primer: 5'-CTAGAGGTGGAAAAAAACAACTAGAGATGGG-3' Tm: 59.958
Expected PCR Product Length: 446
Expected CelI Cleavage Product Sizes: 144, 302
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr3:89962576-89962621

Rank: 132	Homology Score: 10	RVD Match Score: 2.897	Left Half-Site RVD Score: 6.631	Right Half-Site RVD Score: 4.189
	Genomic Region: Intron	Closest Gene: POTEF
Match Type: R29R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-AGGAT TGCAGCCAGCTCCTGC       CCCGGTGCAGTGTATACAGTGTATACTGC        ACAGGTGTTGGGTGCA CCAAA-3'
	3'-TCCTA ACGTCGGTCGAGGACG       GGGCCACGTCACATATGTCACATATGACG        TGTCCACAACCCACGT GGTTT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Warning! Primer pair was generated using suboptimal parameters. 
Primer Index: 002-130879063
Forward Primer: 5'-AAGACGCCCACCGAGCCCA-3' Tm: 62.008
Reverse Primer: 5'-GCTAATGGGTGCAAAGTTTCTTTTAAGGAGCATA-3' Tm: 59.668
Expected PCR Product Length: 450
Expected CelI Cleavage Product Sizes: 144, 306
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr2:130879175-130879235

Rank: 133	Homology Score: 10	RVD Match Score: 2.897	Left Half-Site RVD Score: 4.189	Right Half-Site RVD Score: 6.631
	Genomic Region: Intergenic	Closest Gene: POTEE
Match Type: R29R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TTTGG TGCACCCAACACCTGT       GCAGTATACACTGTATACACTGCACCGGG        GCAGGAGCTGGCTGCA ATCCT-3'
	3'-AAACC ACGTGGGTTGTGGACA       CGTCATATGTGACATATGTGACGTGGCCC        CGTCCTCGACCGACGT TAGGA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Warning! Primer pair was generated using suboptimal parameters. 
Primer Index: 002-131974522
Forward Primer: 5'-GCTAATGGGTGCAAAGTTTCTTTTAAGGAGCATA-3' Tm: 59.668
Reverse Primer: 5'-AAGACGCCCACCGAGCCCA-3' Tm: 62.008
Expected PCR Product Length: 450
Expected CelI Cleavage Product Sizes: 309, 141
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr2:131974799-131974859

Rank: 134	Homology Score: 2	RVD Match Score: 2.898	Left Half-Site RVD Score: 6.136	Right Half-Site RVD Score: 4.603
	Genomic Region: Intergenic	Closest Gene: GPR12
Match Type: R24R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GAGGG AGCAGCAGCCTCCTGA          GGAACCACAGACCCTCCAAAGTTG          GCAGGGGTTAGGTGGG CAGGC-3'
	3'-CTCCC TCGTCGTCGGAGGACT          CCTTGGTGTCTGGGAGGTTTCAAC          CGTCCCCAATCCACCC GTCCG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 013-027482613
Forward Primer: 5'-ATCTGGTCAGTGGAGGTTTGGG-3' Tm: 59.258
Reverse Primer: 5'-CTCACTCTTACTCAGTGCCTGC-3' Tm: 59.258
Expected PCR Product Length: 399
Expected CelI Cleavage Product Sizes: 131, 268
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr13:27482714-27482769

Rank: 135	Homology Score: 5	RVD Match Score: 2.898	Left Half-Site RVD Score: 5.287	Right Half-Site RVD Score: 5.399
	Genomic Region: Intron	Closest Gene: ADAMTS2
Match Type: R19R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CCCCT GACCCCTGACCCCTGG            CACTCTGCCCATTGATCCC             AGAGGAGCCAGGTCCT GAGGA-3'
	3'-GGGGA CTGGGGACTGGGGACC            GTGAGACGGGTAACTAGGG             TCTCCTCGGTCCAGGA CTCCT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 005-178559029
Forward Primer: 5'-AACCCTGGCCTCTCCACAAG-3' Tm: 59.508
Reverse Primer: 5'-GCCTTCCCTTCTCAGTTTTCTGTG-3' Tm: 59.038
Expected PCR Product Length: 327
Expected CelI Cleavage Product Sizes: 145, 182
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr5:178559147-178559197

Rank: 136	Homology Score: 2	RVD Match Score: 2.898	Left Half-Site RVD Score: 5.605	Right Half-Site RVD Score: 5.088
	Genomic Region: Exon	Closest Gene: ADAM11
Match Type: R25R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-AAAAT GGCACCTTCCCCCTTT         CAAGAAGGGTGATTCTGGGGCCGAC          TCAGGGTTTAGGTGCC CCCTG-3'
	3'-TTTTA CCGTGGAAGGGGGAAA         GTTCTTCCCACTAAGACCCCGGCTG          AGTCCCAAATCCACGG GGGAC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 017-042857853
Forward Primer: 5'-GTCCCAGATCGTCTCCAATTCG-3' Tm: 59.258
Reverse Primer: 5'-CAGAGACCCTAACCCTCCAG-3' Tm: 59.508
Expected PCR Product Length: 273
Expected CelI Cleavage Product Sizes: 140, 133
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr17:42857963-42858019

Rank: 137	Homology Score: 5	RVD Match Score: 2.898	Left Half-Site RVD Score: 7.416	Right Half-Site RVD Score: 5.538
	Genomic Region: Intron	Closest Gene: SOGA2
Match Type: L12R
	           Left Nuclease
	         TCACCTTGCCCCACAGGGCAGT
	5'-TACCT TCACATTGCCCCTGAGGCCAGT          GAAGCTTGCCCA                GCATGAGTCAGTTGGC TAAGA-3'
	3'-ATGGA AGTGTAACGGGGACTCCGGTCA          CTTCGAACGGGT                CGTACTCAGTCAACCG ATTCT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 018-008794462
Forward Primer: 5'-AAGAAGGCTCTCCAGGAGGG-3' Tm: 59.508
Reverse Primer: 5'-GTCTGGTCTCTGGTCTCTTAGTC-3' Tm: 59.148
Expected PCR Product Length: 344
Expected CelI Cleavage Product Sizes: 143, 201
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr18:8794578-8794627

Rank: 138	Homology Score: 8	RVD Match Score: 2.899	Left Half-Site RVD Score: 4.936	Right Half-Site RVD Score: 5.776
	Genomic Region: Intron	Closest Gene: TEX35
Match Type: R16R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CTGCC TGCACCACACCCCTCT              GAGGTCTTGTCACCAA              GCAGGACTCAGGTCCC TGCTG-3'
	3'-GACGG ACGTGGTGTGGGGAGA              CTCCAGAACAGTGGTT              CGTCCTGAGTCCAGGG ACGAC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 001-178491197
Forward Primer: 5'-GTCTCAGGAGAGACCCTGCT-3' Tm: 59.508
Reverse Primer: 5'-GAAGGAATGTTCCCTTTGGGGGT-3' Tm: 59.148
Expected PCR Product Length: 276
Expected CelI Cleavage Product Sizes: 146, 130
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr1:178491317-178491364

Rank: 139	Homology Score: 2	RVD Match Score: 2.9	Left Half-Site RVD Score: 5.318	Right Half-Site RVD Score: 7.746
	Genomic Region: Intergenic	Closest Gene: SLC24A2
Match Type: R24L
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-AAGCT GCCACCTGATTCCTCA          TTCAATCACCACCCTACTCATTCA    CCTGTCCTGGGGTGCAAGCTGA GTGGC-3'
	3'-TTCGA CGGTGGACTAAGGAGT          AAGTTAGTGGTGGGATGAGTAAGT    GGACAGGACCCCACGTTCGACT CACCG-5'
	                                                               TGACGGGACACCCCGTTCCACT
	                                                                         Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 009-019935343
Forward Primer: 5'-CCTTGCTGCTGAAGAGGATCTG-3' Tm: 59.258
Reverse Primer: 5'-AGGAACCTACCCTCCTATGCC-3' Tm: 59.378
Expected PCR Product Length: 325
Expected CelI Cleavage Product Sizes: 195, 130
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr9:19935505-19935566

Rank: 140	Homology Score: 10	RVD Match Score: 2.9	Left Half-Site RVD Score: 4.122	Right Half-Site RVD Score: 6.74
	Genomic Region: Intergenic	Closest Gene: TEX35
Match Type: R19R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-AACAC AGCATCTCACTCCTGT            CAACCTGGTGGCCAATGAC             AACAGAGTGAGGGGCA GTTGG-3'
	3'-TTGTG TCGTAGAGTGAGGACA            GTTGGACCACCGGTTACTG             TTGTCTCACTCCCCGT CAACC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 001-178499007
Forward Primer: 5'-CACTTATCATGTCGAGGCCTGC-3' Tm: 59.258
Reverse Primer: 5'-CCCTCTTAATTGCTCCCAGATGAC-3' Tm: 59.038
Expected PCR Product Length: 334
Expected CelI Cleavage Product Sizes: 199, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr1:178499179-178499229

Rank: 141	Homology Score: 5	RVD Match Score: 2.901	Left Half-Site RVD Score: 5.48	Right Half-Site RVD Score: 5.228
	Genomic Region: Intron	Closest Gene: GALNT14
Match Type: R22R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CCACA GAGACCTGGCTCCTCT           TCTCCCACTTCTTTATGCAGCC           AAAGGTGTCAGGTACC AGAAA-3'
	3'-GGTGT CTCTGGACCGAGGAGA           AGAGGGTGAAGAAATACGTCGG           TTTCCACAGTCCATGG TCTTT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 002-031143947
Forward Primer: 5'-GACAGGAAGGTCAGCTGGGA-3' Tm: 59.508
Reverse Primer: 5'-GAATCTGTGTGTGGGCAGTGCT-3' Tm: 59.258
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 135, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr2:31144053-31144106

Rank: 142	Homology Score: 13	RVD Match Score: 2.901	Left Half-Site RVD Score: 4.66	Right Half-Site RVD Score: 6.096
	Genomic Region: Intron	Closest Gene: HPSE2
Match Type: R14R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CAGGC TCCACATGCCTCCTGT               GTGAAATCCCCCCA               ACAAGACTCAGATGCC TCAGG-3'
	3'-GTCCG AGGTGTACGGAGGACA               CACTTTAGGGGGGT               TGTTCTGAGTCTACGG AGTCC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 010-100332368
Forward Primer: 5'-GAGAGTGAACTCACCTTCCATGAC-3' Tm: 59.038
Reverse Primer: 5'-TGGGCACACAGTCAGTTCCTAG-3' Tm: 59.258
Expected PCR Product Length: 304
Expected CelI Cleavage Product Sizes: 174, 130
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr10:100332517-100332562

Rank: 143	Homology Score: 2	RVD Match Score: 2.902	Left Half-Site RVD Score: 4.603	Right Half-Site RVD Score: 6.169
	Genomic Region: Intron	Closest Gene: MTUS2
Match Type: R22R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GAGAT AGCACCTCACACCCAT           TAAGATGGCTGTTATCAAAAAG           ACAGAAGATAAGTGCT CATAA-3'
	3'-CTCTA TCGTGGAGTGTGGGTA           ATTCTACCGACAATAGTTTTTC           TGTCTTCTATTCACGA GTATT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 013-029811755
Forward Primer: 5'-GGCTTCAGCTTCTACTCTGGG-3' Tm: 59.378
Reverse Primer: 5'-CCTGTCATGAGGTTGCTGGATC-3' Tm: 59.258
Expected PCR Product Length: 444
Expected CelI Cleavage Product Sizes: 274, 170
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr13:29812000-29812053

Rank: 144	Homology Score: 5	RVD Match Score: 2.902	Left Half-Site RVD Score: 4.543	Right Half-Site RVD Score: 6.244
	Genomic Region: Intergenic	Closest Gene: MYOM2
Match Type: R13R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CGTGT GGCACCTGCCCCCTCT               GTGTTTTCCTCTC                ACATGGGCCAGGGGCT CTGTC-3'
	3'-GCACA CCGTGGACGGGGGAGA               CACAAAAGGAGAG                TGTACCCGGTCCCCGA GACAG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 008-002210079
Forward Primer: 5'-GATGTCTGGAAGTCCGAGAAGC-3' Tm: 59.258
Reverse Primer: 5'-GAGGTTTCCCCAAAGTGTCCTTC-3' Tm: 59.148
Expected PCR Product Length: 358
Expected CelI Cleavage Product Sizes: 133, 225
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr8:2210188-2210232

Rank: 145	Homology Score: 5	RVD Match Score: 2.904	Left Half-Site RVD Score: 5.325	Right Half-Site RVD Score: 5.406
	Genomic Region: Intron	Closest Gene: KCNT1
Match Type: R27R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GGTGT GGCTCCTGACCCCTGC        CCCCACCCGCTGTCAGCCTGCACACGC         TCAGGGGACAGGTGTG GCTCC-3'
	3'-CCACA CCGAGGACTGGGGACG        GGGGTGGGCGACAGTCGGACGTGTGCG         AGTCCCCTGTCCACAC CGAGG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 009-138659749
Forward Primer: 5'-CAGGAGAAGCTGTCAGCTGG-3' Tm: 59.508
Reverse Primer: 5'-CTGGACACCCTAGGAGACTCCAA-3' Tm: 61.088
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 133, 137
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr9:138659851-138659909

Rank: 146	Homology Score: 5	RVD Match Score: 2.904	Left Half-Site RVD Score: 5.325	Right Half-Site RVD Score: 5.406
	Genomic Region: Intron	Closest Gene: CNNM3
Match Type: R15R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GCCAC CGCACCTGGCCCCTGC              CTAGTTCTCTTGTGT               TGAGGTGTTAGGGGCA GCTGC-3'
	3'-CGGTG GCGTGGACCGGGGACG              GATCAAGAGAACACA               ACTCCACAATCCCCGT CGACG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 002-097493184
Forward Primer: 5'-TAGTAGAGGCGGGGTTTTGCCA-3' Tm: 59.258
Reverse Primer: 5'-AAAAAGGGACAGTGTCCACTGGG-3' Tm: 59.148
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 135, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr2:97493294-97493340

Rank: 147	Homology Score: 5	RVD Match Score: 2.904	Left Half-Site RVD Score: 4.264	Right Half-Site RVD Score: 6.594
	Genomic Region: Intron	Closest Gene: KIAA1199
Match Type: R16R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GCCTG CACAGCTCACTCCTGT              CCAGCCCTGGGATACC              AGGTGAGTCAGGAGAA TGACT-3'
	3'-CGGAC GTGTCGAGTGAGGACA              GGTCGGGACCCTATGG              TCCACTCAGTCCTCTT ACTGA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 015-081145141
Forward Primer: 5'-GCGTTAAGTGTCTGGAAGGCG-3' Tm: 59.378
Reverse Primer: 5'-GGAACTCTCAGCCACAACAAACAC-3' Tm: 59.038
Expected PCR Product Length: 339
Expected CelI Cleavage Product Sizes: 198, 141
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr15:81145313-81145360

Rank: 148	Homology Score: 5	RVD Match Score: 2.905	Left Half-Site RVD Score: 5.734	Right Half-Site RVD Score: 5.014
	Genomic Region: Intergenic	Closest Gene: ELOVL6
Match Type: R11R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TAAAG AATACCTGATCCCTGT                TTCCTAAGAGA                 ATAGGACTCAGGTACC TACTT-3'
	3'-ATTTC TTATGGACTAGGGACA                AAGGATTCTCT                 TATCCTGAGTCCATGG ATGAA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 004-111129424
Forward Primer: 5'-GACCAGTATGGGAAGTCCTGATG-3' Tm: 59.148
Reverse Primer: 5'-CCGTGGGGTAAATACAGGTCAAC-3' Tm: 59.148
Expected PCR Product Length: 327
Expected CelI Cleavage Product Sizes: 143, 184
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr4:111129544-111129586

Rank: 149	Homology Score: 2	RVD Match Score: 2.905	Left Half-Site RVD Score: 5.281	Right Half-Site RVD Score: 5.456
	Genomic Region: Intron	Closest Gene: LGMN
Match Type: R12R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TCTGT CCCCCCATACTCCTGT                GTGGCAGGGAGC                ATAGGAGGCAGGGCCG TGCTC-3'
	3'-AGACA GGGGGGTATGAGGACA                CACCGTCCCTCG                TATCCTCCGTCCCGGC ACGAG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 014-093175798
Forward Primer: 5'-CTCCCTACTACCGAAGAGGTC-3' Tm: 59.378
Reverse Primer: 5'-GATCTGGAGGAGTCCAGGCA-3' Tm: 59.508
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 131, 139
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr14:93175905-93175948

Rank: 150	Homology Score: 5	RVD Match Score: 2.905	Left Half-Site RVD Score: 4.86	Right Half-Site RVD Score: 5.904
	Genomic Region: Intergenic	Closest Gene: MAN1A1
Match Type: R22R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GAGAT ATCACCTCATTCCTGT           CAGGATGGCTATTACTAAAAGG           ACAAGAGACATGTGTT AGCCA-3'
	3'-CTCTA TAGTGGAGTAAGGACA           GTCCTACCGATAATGATTTTCC           TGTTCTCTGTACACAA TCGGT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 006-119691851
Forward Primer: 5'-CCCCCCCAATATCCTGATTGAAC-3' Tm: 59.148
Reverse Primer: 5'-CCCAGAAAAGGGATCACTGCATCA-3' Tm: 59.038
Expected PCR Product Length: 341
Expected CelI Cleavage Product Sizes: 175, 166
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr6:119691997-119692050

Rank: 151	Homology Score: 17	RVD Match Score: 2.905	Left Half-Site RVD Score: 7.722	Right Half-Site RVD Score: 3.443
	Genomic Region: Intergenic	Closest Gene: GSTM3
Match Type: R15R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CCTAC TGCTGCTGTCTCCTTC              TAATTCTGTCGGACA               ACAGGAGTCAGGTTCT GAGTC-3'
	3'-GGATG ACGACGACAGAGGAAG              ATTAAGACAGCCTGT               TGTCCTCAGTCCAAGA CTCAG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 001-110268884
Forward Primer: 5'-CAGGCAGGCAATCTTGATGTGG-3' Tm: 59.258
Reverse Primer: 5'-CTCCTTTTTCCTGCGTAGCAAGTG-3' Tm: 59.038
Expected PCR Product Length: 283
Expected CelI Cleavage Product Sizes: 152, 131
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr1:110269011-110269057

Rank: 152	Homology Score: 5	RVD Match Score: 2.905	Left Half-Site RVD Score: 5.044	Right Half-Site RVD Score: 5.707
	Genomic Region: Promoter	Closest Gene: MMP7
Match Type: R24R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-AGAAC ATCACCAAAATCCTGT          GGTTCTCCATACAGGGGAAAGATG          GCAGGAGGCAGATGGA AATGC-3'
	3'-TCTTG TAGTGGTTTTAGGACA          CCAAGAGGTATGTCCCCTTTCTAC          CGTCCTCCGTCTACCT TTACG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 011-102401741
Forward Primer: 5'-GACTGCTCTCATAGGTATCATTCAGGAC-3' Tm: 60.298
Reverse Primer: 5'-GAAGAATTAGAGGCAGTGTTCCCC-3' Tm: 59.038
Expected PCR Product Length: 445
Expected CelI Cleavage Product Sizes: 257, 188
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr11:102401968-102402023

Rank: 153	Homology Score: 5	RVD Match Score: 2.906	Left Half-Site RVD Score: 6.083	Right Half-Site RVD Score: 4.705
	Genomic Region: Intron	Closest Gene: TEX11
Match Type: R28R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CCTGG GTCTCCTGCCTCCTGA        GGAGATGGATAAAGATGGGCTTCCTCTC        ATGGGAGTCAGGCGTA GACTT-3'
	3'-GGACC CAGAGGACGGAGGACT        CCTCTACCTATTTCTACCCGAAGGAGAG        TACCCTCAGTCCGCAT CTGAA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 00X-070076863
Forward Primer: 5'-CAGGTGTGGTAAAGTGAACTGGG-3' Tm: 59.148
Reverse Primer: 5'-GACACTAGTGGCACTTGAAGGAG-3' Tm: 59.148
Expected PCR Product Length: 434
Expected CelI Cleavage Product Sizes: 163, 271
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chrX:70076994-70077053

Rank: 154	Homology Score: 10	RVD Match Score: 2.906	Left Half-Site RVD Score: 6.722	Right Half-Site RVD Score: 4.174
	Genomic Region: Intron	Closest Gene: TOX
Match Type: R22R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-AAAGG TCACACTGACTCTTGT           GTGGAGAATGTATCATGGAGGA           ACAGGAGTCAGGAGGC GAGGC-3'
	3'-TTTCC AGTGTGACTGAGAACA           CACCTCTTACATAGTACCTCCT           TGTCCTCAGTCCTCCG CTCCG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 008-059835610
Forward Primer: 5'-GGGATCTTGGCAGAGCCTGA-3' Tm: 59.508
Reverse Primer: 5'-CAAGTCTCCACAGAAATGCCTCC-3' Tm: 59.148
Expected PCR Product Length: 303
Expected CelI Cleavage Product Sizes: 148, 155
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr8:59835729-59835782

Rank: 155	Homology Score: 5	RVD Match Score: 2.907	Left Half-Site RVD Score: 6.169	Right Half-Site RVD Score: 4.639
	Genomic Region: Intergenic	Closest Gene: PITX2
Match Type: R22R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TTGCT AACACCTGTCTTTTGT           TGCTTTCATAGTAGCCATTCTA           ACAGTTGTGAGGTGCT ATCTC-3'
	3'-AACGA TTGTGGACAGAAAACA           ACGAAAGTATCATCGGTAAGAT           TGTCAACACTCCACGA TAGAG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Warning! Primer pair was generated using suboptimal parameters. 
Primer Index: 004-111619023
Forward Primer: 5'-GGCAGGCATCTCTTCAACACAC-3' Tm: 59.258
Reverse Primer: 5'-CCCAATTAAGAAATGGGCAAAGGATCTAAATAGAC-3' Tm: 59.578
Expected PCR Product Length: 369
Expected CelI Cleavage Product Sizes: 211, 158
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr4:111619205-111619258

Rank: 156	Homology Score: 5	RVD Match Score: 2.908	Left Half-Site RVD Score: 5.346	Right Half-Site RVD Score: 5.414
	Genomic Region: Intergenic	Closest Gene: FUT5
Match Type: R14R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TCTCT GCCCCCTGCCTCCTGC               CTAGTTCCCGTTCT               TCAGGGCTTAGGTGCA GACAG-3'
	3'-AGAGA CGGGGGACGGAGGACG               GATCAAGGGCAAGA               AGTCCCGAATCCACGT CTGTC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 019-005861521
Forward Primer: 5'-ACAGCTGTCCTGTGGATGTGAAG-3' Tm: 59.148
Reverse Primer: 5'-CAGTGTGGCCTGAGCTAGGA-3' Tm: 59.508
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 135, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr19:5861631-5861676

Rank: 157	Homology Score: 8	RVD Match Score: 2.909	Left Half-Site RVD Score: 6.147	Right Half-Site RVD Score: 4.67
	Genomic Region: Intron	Closest Gene: MYBPHL
Match Type: R30R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-ATGTT TTCACATGCTTCCTGT       GATACTCAGTCCCAGAGCTCTCTGTAAGGA       AGAGGAGTTAGGAGGA AGAAA-3'
	3'-TACAA AAGTGTACGAAGGACA       CTATGAGTCAGGGTCTCGAGAGACATTCCT       TCTCCTCAATCCTCCT TCTTT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 001-109847447
Forward Primer: 5'-GCAGGCTTGCCAAACTAAAAGCTC-3' Tm: 59.038
Reverse Primer: 5'-TCAGAGAGCCAACAGAGCCC-3' Tm: 59.508
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 133, 137
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr1:109847547-109847608

Rank: 158	Homology Score: 5	RVD Match Score: 2.909	Left Half-Site RVD Score: 4.898	Right Half-Site RVD Score: 5.893
	Genomic Region: Intergenic	Closest Gene: SALL4
Match Type: R22R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-AAACC CCCACCAAACTCCTGG           TCAACCTCAAATCTGCATTCTT           ACAGGATTCACCTGCT TGGCT-3'
	3'-TTTGG GGGTGGTTTGAGGACC           AGTTGGAGTTTAGACGTAAGAA           TGTCCTAAGTGGACGA ACCGA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 020-050528903
Forward Primer: 5'-TTGCTTGAACCCAGGAGGCG-3' Tm: 59.508
Reverse Primer: 5'-GGGTTGGATGGGAAAGCAGAG-3' Tm: 59.378
Expected PCR Product Length: 271
Expected CelI Cleavage Product Sizes: 136, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr20:50529010-50529063

Rank: 159	Homology Score: 10	RVD Match Score: 2.91	Left Half-Site RVD Score: 6.642	Right Half-Site RVD Score: 4.262
	Genomic Region: Intergenic	Closest Gene: NDST3
Match Type: R26R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TTACA TGCATCTATCTCATTT         AATGCTTTTAACAATGCTACAACTAA         AAAGTAGTCAGGTGTA GACTG-3'
	3'-AATGT ACGTAGATAGAGTAAA         TTACGAAAATTGTTACGATGTTGATT         TTTCATCAGTCCACAT CTGAC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Warning! Primer pair was generated using suboptimal parameters. 
Primer Index: 004-118702135
Forward Primer: 5'-GCAGGGACACTGGTGTTATGTCT-3' Tm: 59.148
Reverse Primer: 5'-GATAGTAACTTATTTAGAATTGAGGTTGTGTGATGGAG-3' Tm: 59.358
Expected PCR Product Length: 429
Expected CelI Cleavage Product Sizes: 293, 136
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr4:118702397-118702454

Rank: 160	Homology Score: 5	RVD Match Score: 2.911	Left Half-Site RVD Score: 4.705	Right Half-Site RVD Score: 6.128
	Genomic Region: Intron	Closest Gene: FHIT
Match Type: R20R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CTGCT TACACCTGTCTCCTAA            TAAAATCAACTGCCAATCAC            AGGGGAGGCTGGGGCA TTTTA-3'
	3'-GACGA ATGTGGACAGAGGATT            ATTTTAGTTGACGGTTAGTG            TCCCCTCCGACCCCGT AAAAT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 003-060011649
Forward Primer: 5'-GTACTGCCAGAGTGTCAAGAAAGC-3' Tm: 59.038
Reverse Primer: 5'-TAGGAAGAGGACAGTAGGCTAGG-3' Tm: 59.148
Expected PCR Product Length: 286
Expected CelI Cleavage Product Sizes: 156, 130
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr3:60011777-60011828

Rank: 161	Homology Score: 5	RVD Match Score: 2.912	Left Half-Site RVD Score: 5.442	Right Half-Site RVD Score: 5.346
	Genomic Region: Intergenic	Closest Gene: MRPS30
Match Type: R30R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-AAGAG ACCCCATGACCCCTGT       GGACACGAGCTGTCAGGGAGAGCTGCTTAG       AGAGGTGGCAGGGGCA GGCCT-3'
	3'-TTCTC TGGGGTACTGGGGACA       CCTGTGCTCGACAGTCCCTCTCGACGAATC       TCTCCACCGTCCCCGT CCGGA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 005-044679170
Forward Primer: 5'-TCCTTACTAGCCCCCATTAACTCC-3' Tm: 59.038
Reverse Primer: 5'-AATGTCTCCTAGAGGAGCATGGC-3' Tm: 59.148
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 132, 138
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr5:44679269-44679330

Rank: 162	Homology Score: 5	RVD Match Score: 2.912	Left Half-Site RVD Score: 4.571	Right Half-Site RVD Score: 6.288
	Genomic Region: Intron	Closest Gene: SEC14L1
Match Type: R27R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GTTGG AGCACTTAACTCCTGA        ATTTAGTTTATTTCATGGTTATATTGA         ACAAGTTTCATGTGCT ACTTT-3'
	3'-CAACC TCGTGAATTGAGGACT        TAAATCAAATAAAGTACCAATATAACT         TGTTCAAAGTACACGA TGAAA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 017-075205760
Forward Primer: 5'-CTGTGCTTGATCAGAGTGCCTGG-3' Tm: 61.088
Reverse Primer: 5'-TTGCTTGAACCCATAAGGCGGAG-3' Tm: 59.148
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 135, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr17:75205864-75205922

Rank: 163	Homology Score: 5	RVD Match Score: 2.912	Left Half-Site RVD Score: 4.571	Right Half-Site RVD Score: 6.291
	Genomic Region: Intergenic	Closest Gene: EBF1
Match Type: R27R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-AGCTA GGCACCTGACATCTAT        TTTATTTCATCTTAACAATGGTTAATG         ATACGACACAGGTGCT ATTAT-3'
	3'-TCGAT CCGTGGACTGTAGATA        AAATAAAGTAGAATTGTTACCAATTAC         TATGCTGTGTCCACGA TAATA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 005-157749065
Forward Primer: 5'-GCATGGAAGAGCATGGTAGGG-3' Tm: 59.378
Reverse Primer: 5'-GTCAGGTATACTAGGTGCTGTGG-3' Tm: 59.148
Expected PCR Product Length: 290
Expected CelI Cleavage Product Sizes: 159, 131
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr5:157749193-157749251

Rank: 164	Homology Score: 2	RVD Match Score: 2.912	Left Half-Site RVD Score: 5.013	Right Half-Site RVD Score: 5.795
	Genomic Region: Intergenic	Closest Gene: LHX2
Match Type: R11R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TCTCG AGCCCCCACCTCCTGT                TGGATCTTTGG                 GTAGGTGGCAGGGGCA GGAGG-3'
	3'-AGAGC TCGGGGGTGGAGGACA                ACCTAGAAACC                 CATCCACCGTCCCCGT CCTCC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 009-126860975
Forward Primer: 5'-TGTGCGTGAGTCATTACCAGGAAC-3' Tm: 59.038
Reverse Primer: 5'-CCCTGTGGTGAGCATGAAGC-3' Tm: 59.508
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 130, 140
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr9:126861082-126861124

Rank: 165	Homology Score: 2	RVD Match Score: 2.913	Left Half-Site RVD Score: 4.387	Right Half-Site RVD Score: 6.512
	Genomic Region: Intergenic	Closest Gene: B3GNT2
Match Type: R30R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TTTCT GGCACATAACTCCCAT       AGCCCTTGTTACAGTCTTTTGTTGGGTGTA       TTAGGCCTCAGGGGCA GGCCT-3'
	3'-AAAGA CCGTGTATTGAGGGTA       TCGGGAACAATGTCAGAAAACAACCCACAT       AATCCGGAGTCCCCGT CCGGA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Warning! Primer pair was generated using suboptimal parameters. 
Primer Index: 002-062470613
Forward Primer: 5'-CAATCAGGAATGAAAGAGGTGACTTCCTTAG-3' Tm: 59.958
Reverse Primer: 5'-GGTGGGAGTTCACATAGGAGG-3' Tm: 59.378
Expected PCR Product Length: 274
Expected CelI Cleavage Product Sizes: 144, 130
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr2:62470724-62470785

Rank: 166	Homology Score: 8	RVD Match Score: 2.913	Left Half-Site RVD Score: 4.624	Right Half-Site RVD Score: 6.232
	Genomic Region: Intergenic	Closest Gene: PISRT1
Match Type: R11R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GTGAT TACCCCTGACTCCACT                CCTGGATGAGG                 TCAGGGGTCATCTGCA CAGAC-3'
	3'-CACTA ATGGGGACTGAGGTGA                GGACCTACTCC                 AGTCCCCAGTAGACGT GTCTG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 003-138953648
Forward Primer: 5'-CTTGGTGTCTGAGTGGCAAAACTG-3' Tm: 59.038
Reverse Primer: 5'-TATGGGCCTGTTTTACACGGGCCT-3' Tm: 60.908
Expected PCR Product Length: 281
Expected CelI Cleavage Product Sizes: 148, 133
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr3:138953773-138953815

Rank: 167	Homology Score: 5	RVD Match Score: 2.913	Left Half-Site RVD Score: 4.72	Right Half-Site RVD Score: 6.128
	Genomic Region: Intergenic	Closest Gene: TBC1D22A
Match Type: R17R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TGAGG CAAACCTCACTCCAGT             TTCTGGGGCAGCCAGAC              ACAGGGGACTGGGGCA GGAGC-3'
	3'-ACTCC GTTTGGAGTGAGGTCA             AAGACCCCGTCGGTCTG              TGTCCCCTGACCCCGT CCTCG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 022-047592728
Forward Primer: 5'-AATGAGGCCTGGGAGCAGGA-3' Tm: 59.508
Reverse Primer: 5'-CACCAAGAGCACCCCAAAGG-3' Tm: 59.508
Expected PCR Product Length: 274
Expected CelI Cleavage Product Sizes: 133, 141
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr22:47592835-47592883

Rank: 168	Homology Score: 8	RVD Match Score: 2.914	Left Half-Site RVD Score: 5.45	Right Half-Site RVD Score: 5.354
	Genomic Region: Exon	Closest Gene: DMBT1
Match Type: R28R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CCTGT AGGACCTGAATCCAGT        TTGGCCCTGAGGCTGGTGAATGGAGGTG        ACAGGTGTCAGGGCCG AGTGG-3'
	3'-GGACA TCCTGGACTTAGGTCA        AACCGGGACTCCGACCACTTACCTCCAC        TGTCCACAGTCCCGGC TCACC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 010-124341992
Forward Primer: 5'-GCCCTGGTGACTTTGGCATTTG-3' Tm: 59.258
Reverse Primer: 5'-ATTTCCTGGGGCTGACGTGG-3' Tm: 59.508
Expected PCR Product Length: 303
Expected CelI Cleavage Product Sizes: 154, 149
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr10:124342114-124342173

Rank: 169	Homology Score: 8	RVD Match Score: 2.914	Left Half-Site RVD Score: 5.45	Right Half-Site RVD Score: 5.354
	Genomic Region: Exon	Closest Gene: DMBT1
Match Type: R28R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CCTAT AGGACCTGAATCCAGT        TTGGCCCTGAGGCTGGTGAATGGAGGTG        ACAGGTGTCAGGGCCG AGTGG-3'
	3'-GGATA TCCTGGACTTAGGTCA        AACCGGGACTCCGACCACTTACCTCCAC        TGTCCACAGTCCCGGC TCACC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 010-124348357
Forward Primer: 5'-CCAGGTGACTTTGGCCATTAGG-3' Tm: 59.258
Reverse Primer: 5'-ATTTCCTGGGGCTGACGTGG-3' Tm: 59.508
Expected PCR Product Length: 282
Expected CelI Cleavage Product Sizes: 133, 149
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr10:124348458-124348517

Rank: 170	Homology Score: 8	RVD Match Score: 2.914	Left Half-Site RVD Score: 5.45	Right Half-Site RVD Score: 5.354
	Genomic Region: Exon	Closest Gene: DMBT1
Match Type: R28R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CCTGT AGGACCTGAATCCAGT        TTGGCCCTGAGGCTGGTGAATGGAGGTG        ACAGGTGTCAGGGCCG AGTGG-3'
	3'-GGACA TCCTGGACTTAGGTCA        AACCGGGACTCCGACCACTTACCTCCAC        TGTCCACAGTCCCGGC TCACC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 010-124354695
Forward Primer: 5'-GCCCTGGTGACTTTGGCATTTG-3' Tm: 59.258
Reverse Primer: 5'-ATTTCCTGGGGCTGACGTGG-3' Tm: 59.508
Expected PCR Product Length: 303
Expected CelI Cleavage Product Sizes: 154, 149
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr10:124354817-124354876

Rank: 171	Homology Score: 5	RVD Match Score: 2.914	Left Half-Site RVD Score: 5.435	Right Half-Site RVD Score: 5.371
	Genomic Region: Intergenic	Closest Gene: ACOT11
Match Type: R26R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CAGGG TCCATCTGAATCCTCT         GTGATGGGGGAGGGTGTGTGGGGTAC         ACAGGAGAGGGGTGGT GGTGA-3'
	3'-GTCCC AGGTAGACTTAGGAGA         CACTACCCCCTCCCACACACCCCATG         TGTCCTCTCCCCACCA CCACT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 001-054971975
Forward Primer: 5'-GGACCATAATCTCCCCTACAGC-3' Tm: 59.258
Reverse Primer: 5'-GCTGAATTCTGCTACTCCTTGGC-3' Tm: 59.148
Expected PCR Product Length: 276
Expected CelI Cleavage Product Sizes: 132, 144
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr1:54972076-54972133

Rank: 172	Homology Score: 5	RVD Match Score: 2.914	Left Half-Site RVD Score: 5.318	Right Half-Site RVD Score: 5.491
	Genomic Region: Intron	Closest Gene: TMEM248
Match Type: R16R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GAGGA TCCACCCCACTTCTGT              ATCAGATGCTGCAGCA              ACATGAGTTAGGGACT GGAGC-3'
	3'-CTCCT AGGTGGGGTGAAGACA              TAGTCTACGACGTCGT              TGTACTCAATCCCTGA CCTCG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 007-066395352
Forward Primer: 5'-AGAGGCCCCAGCTGGAACTT-3' Tm: 59.508
Reverse Primer: 5'-CAAAGCAGTGCAGGCAGAATGAAC-3' Tm: 59.038
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 132, 138
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr7:66395458-66395505

Rank: 173	Homology Score: 10	RVD Match Score: 2.914	Left Half-Site RVD Score: 4.108	Right Half-Site RVD Score: 6.878
	Genomic Region: Intron	Closest Gene: RBFOX1
Match Type: R12R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GTGGT GGCACCAGACACCTGT                AATCTCAAGTAC                TCAGGAGGCTGAGGCA GGGGA-3'
	3'-CACCA CCGTGGTCTGTGGACA                TTAGAGTTCATG                AGTCCTCCGACTCCGT CCCCT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 016-007614298
Forward Primer: 5'-TTGGGACGCAGAGGTGGGTA-3' Tm: 59.508
Reverse Primer: 5'-CTGGCTTGCAGATGAGTGCCT-3' Tm: 59.378
Expected PCR Product Length: 293
Expected CelI Cleavage Product Sizes: 132, 161
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr16:7614406-7614449

Rank: 174	Homology Score: 5	RVD Match Score: 2.914	Left Half-Site RVD Score: 5.021	Right Half-Site RVD Score: 5.803
	Genomic Region: Intergenic	Closest Gene: CENPC1
Match Type: R18R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TCCTT GCTACCTGACTCATGT             CCACAATGCCCCATAAGC             ACAGAATTCTGGTGGG CCTTG-3'
	3'-AGGAA CGATGGACTGAGTACA             GGTGTTACGGGGTATTCG             TGTCTTAAGACCACCC GGAAC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Warning! Primer pair was generated using suboptimal parameters. 
Primer Index: 004-067641777
Forward Primer: 5'-CTGGTTTAATGCTCTGCTGTTGCC-3' Tm: 59.038
Reverse Primer: 5'-CAGGAATACAGCCATTTGACATTTTCACTGAAAAG-3' Tm: 59.578
Expected PCR Product Length: 377
Expected CelI Cleavage Product Sizes: 171, 206
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr4:67641921-67641970

Rank: 175	Homology Score: 2	RVD Match Score: 2.915	Left Half-Site RVD Score: 5.787	Right Half-Site RVD Score: 5.038
	Genomic Region: Intron	Closest Gene: FAM19A2
Match Type: R10R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CTATG CATACCTGTCACCTGT                 CCATGAGGAG                 AGAGGTGACAGGTGTG CATAG-3'
	3'-GATAC GTATGGACAGTGGACA                 GGTACTCCTC                 TCTCCACTGTCCACAC GTATC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 012-062445219
Forward Primer: 5'-GAGAGGATACAAGGTCAGGTATGG-3' Tm: 59.038
Reverse Primer: 5'-GGCCCATTTTAGAAAACATTTGCTGACTCC-3' Tm: 60.058
Expected PCR Product Length: 446
Expected CelI Cleavage Product Sizes: 271, 175
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr12:62445467-62445508

Rank: 176	Homology Score: 2	RVD Match Score: 2.916	Left Half-Site RVD Score: 4.936	Right Half-Site RVD Score: 5.906
	Genomic Region: Intergenic	Closest Gene: SFTPA2
Match Type: R20R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GATCA CCCACCCCACCCCTGT            AGAACGAGAAGCTCATGAGA            ACAGGGGTCTTGTTCC CCAGC-3'
	3'-CTAGT GGGTGGGGTGGGGACA            TCTTGCTCTTCGAGTACTCT            TGTCCCCAGAACAAGG GGTCG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 010-081345161
Forward Primer: 5'-GAGAAAGGTCTTTGCTAACCCGG-3' Tm: 59.148
Reverse Primer: 5'-GGCTGATTTGAGGACACACTGC-3' Tm: 59.258
Expected PCR Product Length: 294
Expected CelI Cleavage Product Sizes: 164, 130
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr10:81345297-81345348

Rank: 177	Homology Score: 5	RVD Match Score: 2.916	Left Half-Site RVD Score: 5.205	Right Half-Site RVD Score: 5.62
	Genomic Region: Intron	Closest Gene: C20orf27
Match Type: R18R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GGTCC AGAACCTAAATCCAGT             CCCTCCTCCTTCATACCA             CCGGGAGGGAGGTGCA GCCCA-3'
	3'-CCAGG TCTTGGATTTAGGTCA             GGGAGGAGGAAGTATGGT             GGCCCTCCCTCCACGT CGGGT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 020-003747479
Forward Primer: 5'-GAGCAGAAAAATGCCCTACCTCC-3' Tm: 59.148
Reverse Primer: 5'-CCCCCTGAGATGACAAGAATGG-3' Tm: 59.258
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 134, 136
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr20:3747586-3747635

Rank: 178	Homology Score: 5	RVD Match Score: 2.917	Left Half-Site RVD Score: 4.867	Right Half-Site RVD Score: 5.988
	Genomic Region: Exon	Closest Gene: METTL24
Match Type: R23R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CGTAG GTCACCTGCCTCCTGC          TGGCGCCGCGCGGCTGGCCCGGC           GCGGGCGGCAGGTGCG GCCCA-3'
	3'-GCATC CAGTGGACGGAGGACG          ACCGCGGCGCGCCGACCGGGCCG           CGCCCGCCGTCCACGC CGGGT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 006-110679146
Forward Primer: 5'-TTTGCAACTGACCTTCCGGCG-3' Tm: 59.378
Reverse Primer: 5'-GCTGTGCTGTTGTTCGGCCT-3' Tm: 59.508
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 138, 132
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr6:110679255-110679309

Rank: 179	Homology Score: 2	RVD Match Score: 2.917	Left Half-Site RVD Score: 5.309	Right Half-Site RVD Score: 5.523
	Genomic Region: Intron	Closest Gene: DOCK2
Match Type: R12R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TCTTT CTCACCTGATTCCTAC                CAAGTAAACAGG                ACATGTGTTAGGAGCC TGGTT-3'
	3'-AGAAA GAGTGGACTAAGGATG                GTTCATTTGTCC                TGTACACAATCCTCGG ACCAA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 005-169132663
Forward Primer: 5'-CCAGCCTGATTCCTACCTTAGGCA-3' Tm: 60.908
Reverse Primer: 5'-ATAGGTGTGGGAGACTTCAGGTCA-3' Tm: 59.038
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 135, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr5:169132774-169132817

Rank: 180	Homology Score: 8	RVD Match Score: 2.918	Left Half-Site RVD Score: 4.478	Right Half-Site RVD Score: 6.444
	Genomic Region: Intergenic	Closest Gene: ZIC3
Match Type: R25R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-AAGAT ATCACCTCACACCTGT         TAGAAGAGCTTCTGTCAAAAAGACA          AAAGAGGCCAGGTGCA GTGGC-3'
	3'-TTCTA TAGTGGAGTGTGGACA         ATCTTCTCGAAGACAGTTTTTCTGT          TTTCTCCGGTCCACGT CACCG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 00X-136731121
Forward Primer: 5'-GTCACTTTACCTCCCTGAACCTC-3' Tm: 59.148
Reverse Primer: 5'-AGACAGCATTTCGCCATGTTGACC-3' Tm: 59.038
Expected PCR Product Length: 442
Expected CelI Cleavage Product Sizes: 311, 131
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chrX:136731402-136731458

Rank: 181	Homology Score: 5	RVD Match Score: 2.918	Left Half-Site RVD Score: 5.531	Right Half-Site RVD Score: 5.304
	Genomic Region: Intron	Closest Gene: GAB4
Match Type: R30R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TTCTT AAAACCAGACTGCTGT       GTTTTGTCCCAGAGTTTCTGATTCAGTAAG       TCTGGAGTGGGGTGCA GACTG-3'
	3'-AAGAA TTTTGGTCTGACGACA       CAAAACAGGGTCTCAAAGACTAAGTCATTC       AGACCTCACCCCACGT CTGAC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 022-017471581
Forward Primer: 5'-GGGGTCTATCAGCACCAAGC-3' Tm: 59.508
Reverse Primer: 5'-GGGCTCAGCAGATACTTCCTGT-3' Tm: 59.258
Expected PCR Product Length: 388
Expected CelI Cleavage Product Sizes: 151, 237
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr22:17471699-17471760

Rank: 182	Homology Score: 10	RVD Match Score: 2.918	Left Half-Site RVD Score: 5.228	Right Half-Site RVD Score: 5.612
	Genomic Region: Intergenic	Closest Gene: DHRS2
Match Type: R10R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TCTCC TTCACCTGACTCTAGT                 TGTTGCTGGA                 ACAGGACTTTGTTGTA TATTC-3'
	3'-AGAGG AAGTGGACTGAGATCA                 ACAACGACCT                 TGTCCTGAAACAACAT ATAAG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 014-024242484
Forward Primer: 5'-CAAGTCTTCCTCAGCCATCAGACA-3' Tm: 59.038
Reverse Primer: 5'-CCAGACACATCAACAGAGTGGAAG-3' Tm: 59.038
Expected PCR Product Length: 298
Expected CelI Cleavage Product Sizes: 138, 160
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr14:24242599-24242640

Rank: 183	Homology Score: 5	RVD Match Score: 2.918	Left Half-Site RVD Score: 5.348	Right Half-Site RVD Score: 5.489
	Genomic Region: Intergenic	Closest Gene: GALNTL4
Match Type: R15R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-AGGCA GCCACAGGACTCCTCT              GGCTGTCCATCCAGC               ATAGCACTCAGGTGGA GGCAG-3'
	3'-TCCGT CGGTGTCCTGAGGAGA              CCGACAGGTAGGTCG               TATCGTGAGTCCACCT CCGTC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 011-011275773
Forward Primer: 5'-TCCACTGTGGCCAGAGCTAC-3' Tm: 59.508
Reverse Primer: 5'-GTACCTGGAAGCCCCTCTGA-3' Tm: 59.508
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 138, 132
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr11:11275886-11275932

Rank: 184	Homology Score: 5	RVD Match Score: 2.919	Left Half-Site RVD Score: 5.467	Right Half-Site RVD Score: 5.377
	Genomic Region: Exon	Closest Gene: LELP1
Match Type: R24R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TAACA TCCACCTCACTCAACT          GCTTGTCAAGTTCACCACCAACAC          AGAGGGGTAAGTTGCA TTTGG-3'
	3'-ATTGT AGGTGGAGTGAGTTGA          CGAACAGTTCAAGTGGTGGTTGTG          TCTCCCCATTCAACGT AAACC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 001-153175860
Forward Primer: 5'-CTCATAAGTGGAGGTCATTTCCCC-3' Tm: 59.038
Reverse Primer: 5'-GCACACACCAGTTCTTACCACTGA-3' Tm: 59.038
Expected PCR Product Length: 300
Expected CelI Cleavage Product Sizes: 132, 168
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr1:153175962-153176017

Rank: 185	Homology Score: 5	RVD Match Score: 2.919	Left Half-Site RVD Score: 6.845	Right Half-Site RVD Score: 4.166
	Genomic Region: Intergenic	Closest Gene: MATN1
Match Type: R30R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TGGAG AGCTCCTGAGTCCGGA       GGATGATCAGAGGTGAGCTCAGAAGGGCAT       AAAGGAGTCAGGGGTC CACCA-3'
	3'-ACCTC TCGAGGACTCAGGCCT       CCTACTAGTCTCCACTCGAGTCTTCCCGTA       TTTCCTCAGTCCCCAG GTGGT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 001-030487647
Forward Primer: 5'-CCATTGTCCTGAGGATGAACTGTC-3' Tm: 59.038
Reverse Primer: 5'-TCTGCACCCATGGGCTGAGT-3' Tm: 59.508
Expected PCR Product Length: 307
Expected CelI Cleavage Product Sizes: 166, 141
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr1:30487780-30487841

Rank: 186	Homology Score: 2	RVD Match Score: 2.92	Left Half-Site RVD Score: 5.362	Right Half-Site RVD Score: 5.491
	Genomic Region: Intergenic	Closest Gene: MIS18BP1
Match Type: R23R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CTCAC AACACCTTTCTCCAGT          AGTATCATCACCCCACCTACCTA           ACAGAAGTTAGGGTCT CTACC-3'
	3'-GAGTG TTGTGGAAAGAGGTCA          TCATAGTAGTGGGGTGGATGGAT           TGTCTTCAATCCCAGA GATGG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 014-046262053
Forward Primer: 5'-GTCTTTACCCTCCACCCTTGTTTC-3' Tm: 59.038
Reverse Primer: 5'-GGAGCTTGAGTTTTCAGGCTGTTC-3' Tm: 59.038
Expected PCR Product Length: 356
Expected CelI Cleavage Product Sizes: 212, 144
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr14:46262236-46262290

Rank: 187	Homology Score: 5	RVD Match Score: 2.921	Left Half-Site RVD Score: 5.354	Right Half-Site RVD Score: 5.502
	Genomic Region: Exon	Closest Gene: FOXO4
Match Type: R27R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TTCAA GGCCCCTGACAGCTGT        CCCTGCCCTTCTTCCCCTTCCCTGACT         GCAGGGGTTATGTGGA AGTGT-3'
	3'-AAGTT CCGGGGACTGTCGACA        GGGACGGGAAGAAGGGGAAGGGACTGA         CGTCCCCAATACACCT TCACA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 00X-070322882
Forward Primer: 5'-CCTGGAATTAGCTTGGGGCC-3' Tm: 59.508
Reverse Primer: 5'-TGATCCACATCCCCCCCCAT-3' Tm: 59.508
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 134, 136
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chrX:70322985-70323043

Rank: 188	Homology Score: 5	RVD Match Score: 2.921	Left Half-Site RVD Score: 5.338	Right Half-Site RVD Score: 5.523
	Genomic Region: Intergenic	Closest Gene: PLXNA1
Match Type: R15R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CTATC TGCCTCTCACTCCTCT              CTCTGAGGCCCTGAG               ACAGGTGCCAGGTTTT CATAC-3'
	3'-GATAG ACGGAGAGTGAGGAGA              GAGACTCCGGGACTC               TGTCCACGGTCCAAAA GTATG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 003-126821831
Forward Primer: 5'-CCTGCATTCAGGAAACTTCCGTTG-3' Tm: 59.038
Reverse Primer: 5'-GCTGATGGCTTCTGCCATTCTTG-3' Tm: 59.148
Expected PCR Product Length: 305
Expected CelI Cleavage Product Sizes: 157, 148
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr3:126821963-126822009

Rank: 189	Homology Score: 2	RVD Match Score: 2.922	Left Half-Site RVD Score: 5.147	Right Half-Site RVD Score: 5.723
	Genomic Region: Intron	Closest Gene: TBC1D1
Match Type: R21R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TTAAC AACACGTAGCTCCTGT           CCTGGGGAGGCTCATAGTTTG            ATAGGGGTAAGATGGA AAGAA-3'
	3'-AATTG TTGTGCATCGAGGACA           GGACCCCTCCGAGTATCAAAC            TATCCCCATTCTACCT TTCTT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 004-038097102
Forward Primer: 5'-GCTACCAAATGGAGGTTTGGCTCT-3' Tm: 59.038
Reverse Primer: 5'-CCATGCCTCTACCCATTTTCCTC-3' Tm: 59.148
Expected PCR Product Length: 307
Expected CelI Cleavage Product Sizes: 147, 160
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr4:38097221-38097273

Rank: 190	Homology Score: 2	RVD Match Score: 2.922	Left Half-Site RVD Score: 5.826	Right Half-Site RVD Score: 5.053
	Genomic Region: Intergenic	Closest Gene: MIR563
Match Type: R17R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-AGGGC AGGAACTGACTCCGAT             TTAATTTGGGGTCACAA              TGAGTAGTGAGGTGCT TACAT-3'
	3'-TCCCG TCCTTGACTGAGGCTA             AATTAAACCCCAGTGTT              ACTCATCACTCCACGA ATGTA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 003-015967056
Forward Primer: 5'-CAGGCCATGGTAAGAGGATAGG-3' Tm: 59.258
Reverse Primer: 5'-CCAAACTCCAAGCCCACACAC-3' Tm: 59.378
Expected PCR Product Length: 338
Expected CelI Cleavage Product Sizes: 179, 159
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr3:15967209-15967257

Rank: 191	Homology Score: 8	RVD Match Score: 2.922	Left Half-Site RVD Score: 5.362	Right Half-Site RVD Score: 5.505
	Genomic Region: Intron	Closest Gene: KIAA0317
Match Type: R19R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TTCTC TACTCCTGACACCTTT            GTCTAGACAAAGATCTAGT             ACAGGAATCATTTGCT TCAGG-3'
	3'-AAGAG ATGAGGACTGTGGAAA            CAGATCTGTTTCTAGATCA             TGTCCTTAGTAAACGA AGTCC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 014-075136937
Forward Primer: 5'-GAGGATCAGAGCCCATCCAAATGT-3' Tm: 59.038
Reverse Primer: 5'-GGAACCTGACAACCTGTGTCCT-3' Tm: 59.258
Expected PCR Product Length: 443
Expected CelI Cleavage Product Sizes: 212, 231
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr14:75137122-75137172

Rank: 192	Homology Score: 2	RVD Match Score: 2.923	Left Half-Site RVD Score: 5.038	Right Half-Site RVD Score: 5.848
	Genomic Region: Intron	Closest Gene: SH3GL2
Match Type: R17R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TGAGG CCCACCAAGCTCCTGT             GGCCAGCTAAGCCCAAG              ACAGGTGTTAGGACCT GTGAG-3'
	3'-ACTCC GGGTGGTTCGAGGACA             CCGGTCGATTCGGGTTC              TGTCCACAATCCTGGA CACTC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 009-017690436
Forward Primer: 5'-CCCCACATCACATGTCCTCTTTAG-3' Tm: 59.038
Reverse Primer: 5'-CCTAGTACCCGGCACTTTTCATG-3' Tm: 59.148
Expected PCR Product Length: 308
Expected CelI Cleavage Product Sizes: 146, 162
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr9:17690556-17690604

Rank: 193	Homology Score: 5	RVD Match Score: 2.923	Left Half-Site RVD Score: 5.84	Right Half-Site RVD Score: 5.046
	Genomic Region: Intergenic	Closest Gene: TMEM185A
Match Type: R27R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TTTCA TTCACCTCATTCATAT        CATCCCCACCAAAAGTATAGCCATTTC         AGTGGAGTCAGCTGTA CACCA-3'
	3'-AAAGT AAGTGGAGTAAGTATA        GTAGGGGTGGTTTTCATATCGGTAAAG         TCACCTCAGTCGACAT GTGGT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 00X-148727617
Forward Primer: 5'-GAGTCTTCCATAGCTGAGTGCAAC-3' Tm: 59.038
Reverse Primer: 5'-ACCCAACATCTGCCATCTGCAAG-3' Tm: 59.148
Expected PCR Product Length: 271
Expected CelI Cleavage Product Sizes: 140, 131
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chrX:148727726-148727784

Rank: 194	Homology Score: 5	RVD Match Score: 2.923	Left Half-Site RVD Score: 6.387	Right Half-Site RVD Score: 4.565
	Genomic Region: Intergenic	Closest Gene: UBE2MP1
Match Type: R10R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CTGCT TGCACCTGCCTGCTTG                 GCCATTCCAG                 GCAGGAGTTCGGTGTC TGCTC-3'
	3'-GACGA ACGTGGACGGACGAAC                 CGGTAAGGTC                 CGTCCTCAAGCCACAG ACGAG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 016-034421445
Forward Primer: 5'-GGTGGAAGCAAATATGGGGAGG-3' Tm: 59.258
Reverse Primer: 5'-GGCAGAAATGGTTCCCAGGAAAAG-3' Tm: 59.038
Expected PCR Product Length: 275
Expected CelI Cleavage Product Sizes: 143, 132
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr16:34421565-34421606

Rank: 195	Homology Score: 5	RVD Match Score: 2.924	Left Half-Site RVD Score: 8.057	Right Half-Site RVD Score: 5.281
	Genomic Region: Intron	Closest Gene: EMID1
Match Type: L15R
	           Left Nuclease
	         TCACCTTGCCCCACAGGGCAGT
	5'-ATGCT GCAGGCTGCCCCATAGGGCAGT        CCAAGCTGTCTTGGC               AGAGGTGGCAGGTGAA GACTA-3'
	3'-TACGA CGTCCGACGGGGTATCCCGTCA        GGTTCGACAGAACCG               TCTCCACCGTCCACTT CTGAT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 022-029629152
Forward Primer: 5'-ATTGCAGTGGGGTTAGAACCCACA-3' Tm: 59.038
Reverse Primer: 5'-TGGGGCCAGTGGTTGTTGGT-3' Tm: 59.508
Expected PCR Product Length: 268
Expected CelI Cleavage Product Sizes: 136, 132
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr22:29629260-29629312

Rank: 196	Homology Score: 5	RVD Match Score: 2.924	Left Half-Site RVD Score: 5.442	Right Half-Site RVD Score: 5.435
	Genomic Region: Intergenic	Closest Gene: LOC653501
Match Type: R25R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TGTAC GCCACCTGCCTCAAGT         CCTAATCCCTTCATGTTGGAATTAC          ACTGCAGTCAGGGGTA TCCTT-3'
	3'-ACATG CGGTGGACGGAGTTCA         GGATTAGGGAAGTACAACCTTAATG          TGACGTCAGTCCCCAT AGGAA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 009-039525524
Forward Primer: 5'-GCCCTGTGGATGCCACATTC-3' Tm: 59.508
Reverse Primer: 5'-CTGGAAGGGGCATGGAATATGAG-3' Tm: 59.148
Expected PCR Product Length: 339
Expected CelI Cleavage Product Sizes: 149, 190
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr9:39525643-39525699

Rank: 197	Homology Score: 5	RVD Match Score: 2.924	Left Half-Site RVD Score: 5.442	Right Half-Site RVD Score: 5.435
	Genomic Region: Intergenic	Closest Gene: ZNF658
Match Type: R25R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TGTAC GCCACCTGCCTCAAGT         CCTAATCCCTTCATGTTGGAATTAC          ACTGCAGTCAGGGGTA TCCTT-3'
	3'-ACATG CGGTGGACGGAGTTCA         GGATTAGGGAAGTACAACCTTAATG          TGACGTCAGTCCCCAT AGGAA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 009-040853051
Forward Primer: 5'-GCCCTGTGGATGCCACATTC-3' Tm: 59.508
Reverse Primer: 5'-CTGGAAGGGGCATGGAATATGAG-3' Tm: 59.148
Expected PCR Product Length: 339
Expected CelI Cleavage Product Sizes: 149, 190
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr9:40853170-40853226

Rank: 198	Homology Score: 5	RVD Match Score: 2.924	Left Half-Site RVD Score: 5.435	Right Half-Site RVD Score: 5.442
	Genomic Region: Intergenic	Closest Gene: FAM75A4
Match Type: R19R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-AAGGA TACCCCTGACTGCAGT            GTGATTCCAACATGAAGGG             ACTTGAGGCAGGTGGC GTACA-3'
	3'-TTCCT ATGGGGACTGACGTCA            CACTAAGGTTGTACTTCCC             TGAACTCCGTCCACCG CATGT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 009-041229954
Forward Primer: 5'-GGATTTAATCCACAGGCAAGGAGG-3' Tm: 59.038
Reverse Primer: 5'-GCCCTGTGGATGCCACATTC-3' Tm: 59.508
Expected PCR Product Length: 274
Expected CelI Cleavage Product Sizes: 131, 143
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr9:41230058-41230108

Rank: 199	Homology Score: 5	RVD Match Score: 2.924	Left Half-Site RVD Score: 5.442	Right Half-Site RVD Score: 5.435
	Genomic Region: Intergenic	Closest Gene: LOC653501
Match Type: R25R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TGTAC GCCACCTGCCTCAAGT         CCTAATCCCTTCATGTTGGAATTAC          ACTGCAGTCAGGGGTA TCCTT-3'
	3'-ACATG CGGTGGACGGAGTTCA         GGATTAGGGAAGTACAACCTTAATG          TGACGTCAGTCCCCAT AGGAA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 009-041670537
Forward Primer: 5'-GCCCTGTGGATGCCACATTC-3' Tm: 59.508
Reverse Primer: 5'-CTGGAAGGGGCATGGAATATGAG-3' Tm: 59.148
Expected PCR Product Length: 339
Expected CelI Cleavage Product Sizes: 149, 190
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr9:41670656-41670712

Rank: 200	Homology Score: 5	RVD Match Score: 2.924	Left Half-Site RVD Score: 5.435	Right Half-Site RVD Score: 5.442
	Genomic Region: Intergenic	Closest Gene: FAM75A6
Match Type: R19R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-AAGGA TACCCCTGACTGCAGT            GTGATTCCAACATGAAGGG             ACTTGAGGCAGGTGGC GTACA-3'
	3'-TTCCT ATGGGGACTGACGTCA            CACTAAGGTTGTACTTCCC             TGAACTCCGTCCACCG CATGT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 009-043533250
Forward Primer: 5'-GGATTTAATCCACAGGCAAGGAGG-3' Tm: 59.038
Reverse Primer: 5'-GCCCTGTGGATGCCACATTC-3' Tm: 59.508
Expected PCR Product Length: 274
Expected CelI Cleavage Product Sizes: 131, 143
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr9:43533354-43533404

Plate format Oligo Order Form
Well
Name
Sequence
5' mods
Internal
Notes
Primer Plate #1
A1
011-005248069F
GTGCCTATCAGAAACCCAAGAGTC
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
A2
011-005248069R
CAATCTACTCCCAGGAGCAGG
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
B1
011-005255477F
GTCAGTGCCTATCAGAAACCCAAG
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
B2
011-005255477R
TCACTGGAGCAGGGAGGACA
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
C1
002-205984816F
GTGGGTACTGGCTGCTAAGTATG
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
C2
002-205984816R
GTTCCTAGCAGCCACAGCAC
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
D1
009-094951264F
ATCCAGCCCTGGGACTTTACTTC
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
D2
009-094951264R
TGTGAGTCTCAGAACCAAGGCTGT
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
E1
005-177549655F
GGAACAACACCAGGGAATGCC
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
E2
005-177549655R
GGGAAATGTTCCCATTCCTTGCTG
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
F1
00X-034076224F
AGTATTGCCCTTTCTGCTAGGGAC
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
F2
00X-034076224R
CACTGCACACTTCTGTGGCTC
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
G1
003-126892267F
ACCCAGACCCAGACATCCAC
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
G2
003-126892267R
CTATGTGCAGCTGGAGAAACTGAG
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
H1
006-024980600F
GGGAGTGAAATCAAGACACCTGG
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
H2
006-024980600R
AATGCCCAAAAGCCCCCTGTG
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
A3
003-027142836F
AGGACAGGAAGGAAGAGCCC
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
A4
003-027142836R
GTGTGGATTGGCAACCTCTTCATG
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
B3
006-123066634F
GTAGTGGGATCGTGCCTGTG
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
B4
006-123066634R
TCAGTACACTTGGACCCGGC
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
C3
016-083864892F
GGAAACTGTGGCCCAGAGAG
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
C4
016-083864892R
CTCAGCATCCGTTCCTGTTCTTC
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
D3
022-045287459F
CAAAGGCTCTGCCTGCAGGA
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
D4
022-045287459R
CAGGACTAAGGCCGCTCTTC
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
E3
020-003459687F
GCCAGTGTGCCCAGCCAATT
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
E4
020-003459687R
GTGTACGTAGCTGTCTTCCCTTC
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
F3
008-144606723F
CTAGCAGCAGGCAAGTCGCA
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
F4
008-144606723R
ACACAGAGCATGGGCTTGGG
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
G3
002-199208411F
GCCTTACACATAACCTCACTGCC
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
G4
002-199208411R
CTCCAACATAACTCAGCCTCGAAG
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
H3
006-110284189F
GCAATGTTGCTCCAAGAGGCC
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
H4
006-110284189R
GAACTTATTCCTCCCACCTAGCTG
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
A5
019-039625781F
CCTCACCAACATTTGGTACCGTC
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
A6
019-039625781R
CGGAGGTTGCAGTGAGCTGA
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
B5
001-184575055F
GGTGGTGCTAAACCGAACCTG
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
B6
001-184575055R
GCCACTACACCTGGCCAATG
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
C5
012-072604585F
GTTGAGAGTTGACACCAACTTCTTTTCTTTTTCC
S2_S5_NN_Ranking-RVDs	Warning! Primer pair was generated using suboptimal parameters. 
C6
012-072604585R
CCACTCCTAGTGTGGAACCTC
S2_S5_NN_Ranking-RVDs	Warning! Primer pair was generated using suboptimal parameters. 
D5
004-178644255F
CCCGAAGATGCACTGGCATTAAAG
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
D6
004-178644255R
CCGTAGCCATAGAGTGAGGATGGA
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
E5
012-026617507F
GCACTTGTTGACTATCACAGCCAC
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
E6
012-026617507R
CATTCCTGTAGCCCAAGCCTG
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
F5
005-144614657F
CAGTGACCTTAGCAGACTATCAGC
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
F6
005-144614657R
CTCTGGGCTTGACAGCAACTGA
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
G5
010-029082810F
CCTCTGAGCCCCATTCTCCT
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
G6
010-029082810R
TAGAGATGGGGTCTCACCTTGTTG
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
H5
003-070683485F
GGGCAAAGGATCTGAATAGACAATTTCCCA
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
H6
003-070683485R
CACTGATGGACACTTCTGTTGCC
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
A7
003-058640715F
CATATGAGGAAACTGAGAGGCGG
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
A8
003-058640715R
CCTCGAGGAGGGCTATTCTAGA
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
B7
00Y-018946879F
ATACACTTGGACTCGGCTTCCC
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
B8
00Y-018946879R
GTGAATCTCAGGTAGTGTACCTGC
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
C7
005-171849905F
ATCTTCCACAGGCCAGCCCA
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
C8
005-171849905R
GTTTGCCCGGGCTTGTTATCAATG
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
D7
014-049949953F
GTTGTTCTTTCACCCACTGGAGC
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
D8
014-049949953R
GACACATCAGAAAGACCGCTGAC
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
E7
002-086899302F
GGCTCTGTCTTTTGTTATGTCAAATGAGAAGCA
S2_S5_NN_Ranking-RVDs	Warning! Primer pair was generated using suboptimal parameters. 
E8
002-086899302R
CTCCATCATTAATTCAAACAGCCTTCAATACCC
S2_S5_NN_Ranking-RVDs	Warning! Primer pair was generated using suboptimal parameters. 
F7
002-080981749F
GTCTACTGATAGGGAGAAATATTTGCAAACCATG
S2_S5_NN_Ranking-RVDs	Warning! Primer pair was generated using suboptimal parameters. 
F8
002-080981749R
GTGCCAACCATTAACATACAAAGAGAGATTCACA
S2_S5_NN_Ranking-RVDs	Warning! Primer pair was generated using suboptimal parameters. 
G7
005-002006653F
TCAGCCTCCCCAACCTCAGA
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
G8
005-002006653R
TGTGGAGATGTCGTTACGCAGG
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
H7
dom-000216374F
TCTCCTTTCTGTACCTCAGAGACC
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
H8
dom-000216374R
CCCACCCTGGTACAGCAAATG
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
A9
012-120856642F
CCCTGGAATTGGCTCACTAGCT
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
A10
012-120856642R
CTGGGAGGTGAAGGTTGCAGT
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
B9
012-129191997F
GGTGGGGTATACCAGCATAAACTC
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
B10
012-129191997R
GGCTTCAAGGACCCTTTCTTAGCT
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
C9
006-165292151F
GCTCTGATGGCCTAGAGGTC
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
C10
006-165292151R
GGGCAGGGTAATACTAAGAGACAC
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
D9
012-133001318F
ACACTGCTCAGGAGACCAGG
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
D10
012-133001318R
TTACACAGCCCAGCCAAGCC
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
E9
005-059619945F
CCAAGACTTAGCTTAGCACCCAAC
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
E10
005-059619945R
CATGCCTGGAGTTAGCCTCTCT
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
F9
016-062102796F
CATTGTAGCCTAGACAAGTCACCG
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
F10
016-062102796R
GGGGCAGTGAAGGTATTCAGTATG
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
G9
005-053827309F
GCCACGTGTTGTCTACAGCAAG
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
G10
005-053827309R
CAGCACAAGCACTCTTCGTTATCC
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
H9
014-077327823F
CCAACAGCTGGTAAGGATGTCAAG
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
H10
014-077327823R
CTGTGGAAACACCAAAGCAGGG
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
A11
005-177943719F
AAGCTTTCTGCCTCCTCAGGCC
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
A12
005-177943719R
CACCTCAGTGACAGGCGCTT
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
B11
001-203281134F
TGCATGCTGCAGATTAAGAGCCC
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
B12
001-203281134R
TAAGAAGTACCAGGACCACAACCC
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
C11
015-082621505F
TGGCTTTCACGGGTCTGTGC
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
C12
015-082621505R
TAGACATGCAGCCTGGAGCC
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
D11
015-082918832F
TGGCTTTCACGGGTCTGTGC
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
D12
015-082918832R
TAGACATGCAGCCTGGAGCC
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
E11
015-085803797F
TAGACATGCAGCCTGGAGCC
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
E12
015-085803797R
TGGCTTTCACGGGTCTGTGC
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
F11
011-117354866F
GCTCAGGCAAAGTTGTTCCCAG
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
F12
011-117354866R
ATGTGCTGAGAGGCTGGTGC
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
G11
001-050729484F
AACCAGGCCAGGAAAGCCCA
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
G12
001-050729484R
GGAGACAGGCACATAGCAGTGA
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
H11
008-102329033F
CCAATACACTGAGCTCCCTCC
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
H12
008-102329033R
GAGGAGCAGAACTAGGAACACAG
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
Primer Plate #2
A1
002-038352606F
GCCGCCATGATGAAAACCAAACTC
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
A2
002-038352606R
CCCTCTTCCTACTTCTTGGGTG
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
B1
005-111863094F
AGTACACTTGGACCCAGCTTACC
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
B2
005-111863094R
TGAATCCCAGGTAGCGTACCTGC
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
C1
019-018725301F
GAGGCACAAAGGACAATGTCACC
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
C2
019-018725301R
CCATAAGGCAGTGTGTGCGTG
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
D1
009-105115544F
CTGAGGTGTATCTAACCCCACCTT
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
D2
009-105115544R
CCTTGCTCTAAAGCAGACCTTCAC
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
E1
012-032161181F
GCTCAATGGCAGAAAACCAAATAACCCAAC
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
E2
012-032161181R
GAGACGGGTTTTTGCCATGTTGC
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
F1
012-040234817F
CCTTACAATTCACGGCTCTCAGAC
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
F2
012-040234817R
CCACTGGAGTTGACAGATTTGGC
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
G1
008-015147764F
CCCCACTTCATCTGCTAAACTTCC
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
G2
008-015147764R
GAGGGGGAAAATGTGGGAAGTTCT
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
H1
011-002239013F
AGCTGTCCAGCCTGCAGCAT
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
H2
011-002239013R
TCATATTCCTGGGTGGTCACTCC
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
A3
00X-079108050F
GGTGTCAGTAGCAGATAGGGATG
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
A4
00X-079108050R
CTGTACCCAACCCACTTTATGGC
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
B3
002-011376315F
CCCCTCATGGAGAACACAATACAAAAGAAAC
S2_S5_NN_Ranking-RVDs	Warning! Primer pair was generated using suboptimal parameters. 
B4
002-011376315R
GAGAGAAGAGTTACGCGTCTTCC
S2_S5_NN_Ranking-RVDs	Warning! Primer pair was generated using suboptimal parameters. 
C3
019-042664524F
CAACCCGGTCCAGTCGAGAA
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
C4
019-042664524R
TTCCACAGTGGCAGGCACTAG
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
D3
016-071012605F
CTCCTAGCTTCCTGATTCTTTGCC
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
D4
016-071012605R
CCCACCCTGGTACAGCAAATG
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
E3
016-051386732F
CCCTCCGAAACAACATCTTACCAC
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
E4
016-051386732R
GGGTTGAAGGCAGATATGTTGGG
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
F3
004-016836013F
CACCTATGTGGCGTGATTGAGTGA
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
F4
004-016836013R
AGACAGGGTTTCAGCATGTTGGC
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
G3
017-078085986F
AAAAGCGGAGGCCCAGACCA
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
G4
017-078085986R
ACTCGGGAACCCTGTCACCA
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
H3
017-029816092F
GCCAGTTTTCCTTGACTGACCG
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
H4
017-029816092R
CCATCCCAGCAACCACTGTAAC
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
A5
006-071979511F
GACATTCTAGAGCAGTTTGGCAGG
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
A6
006-071979511R
GTGACTGTGGGTGAAGATGCTG
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
B5
003-143508466F
GGGCTACTGAGACTCACCATATC
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
B6
003-143508466R
GGACTCGGTAACTCACTCAGG
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
C5
018-021451074F
CCTTCCCTCTTTCTCACTCACC
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
C6
018-021451074R
GGGTCTTCCTTAGGCAGGCT
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
D5
215-000126245F
GTCCCCTCTCCTTGCACTTTTC
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
D6
215-000126245R
CCTTGGGAACCTTGGACAATAAGC
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
E5
021-015154271F
GTCCCCTCTCCTTGCACTTTTC
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
E6
021-015154271R
CCTTGGGAACCTTGGACAATAAGC
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
F5
005-178559029F
AACCCTGGCCTCTCCACAAG
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
F6
005-178559029R
ATGTTTGAGATCCCTGCAGGAGC
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
G5
003-077451553F
CTGCACGTGTGTCCCAGAAC
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
G6
003-077451553R
ACACACGCTCACTTGAGGCATTC
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
H5
019-045727578F
GAGAAGGGGAGTGTTCCACTG
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
H6
019-045727578R
GGATAACAGAGGGCCATCAGC
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
A7
005-152047769F
GTGGACAAAGAACCTGAATAGACATTTAGCAAAAG
S2_S5_NN_Ranking-RVDs	Warning! Primer pair was generated using suboptimal parameters. 
A8
005-152047769R
GCAGAGTTCCATTTGAGATATTGACTTCATTTCC
S2_S5_NN_Ranking-RVDs	Warning! Primer pair was generated using suboptimal parameters. 
B7
008-126184331F
CTGAGTGGTGAAGAAACTTGCCC
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
B8
008-126184331R
GCCAAGCTTACCTGTCTAGAACTG
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
C7
00X-069048457F
GTGAGATGAAACCAGTGCCTCAG
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
C8
00X-069048457R
GAACTGAAGGGTACACTAGCTGTC
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
D7
012-101038644F
CTGTGCTCCCCTTTCTGAATAGTG
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
D8
012-101038644R
TACACTTGGACCCGACTTCCC
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
E7
002-016692025F
CTCCTGATAGTAAGGGCTGTCTC
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
E8
002-016692025R
ATCAAAGCTGGCCCTTCCTGAC
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
F7
013-075094100F
ATTGGCTCACGGTTCTGTAGGC
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
F8
013-075094100R
TGGATTGTGGGAGTGGATCCTTC
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
G7
010-095287016F
GAGCTGCTAAAATTGCTTGCTGCC
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
G8
010-095287016R
GCACCCAGCCTGTTCAGCAA
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
H7
014-069492113F
GCCTTCTCACTGAGGCCTTC
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
H8
014-069492113R
TCCCAGAGAGCTTTCTTGCTCC
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
A9
004-180706838F
GCACAGTGGTTCTATTTGCAACACATTTCC
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
A10
004-180706838R
CAAACCACAGACCCAGAAACCTC
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
B9
No PrimersF
No Primers
S2_S5_NN_Ranking-RVDs	No Primers
B10
No PrimersR
No Primers
S2_S5_NN_Ranking-RVDs	No Primers
C9
005-078702362F
GAAAGGATCCTGGATGAGGGG
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
C10
005-078702362R
GTAGGAGTTTCCTGCCTCTGTC
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
D9
005-141162758F
GTGCTTAGGAGGCCATGGTG
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
D10
005-141162758R
GCGAAGACTCAGTAAGTGGTGAC
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
E9
003-137740660F
GAGGGGTGGAAACTCTCCAAAAAC
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
E10
003-137740660R
TGCTGTCCTTGGGAGTCCGT
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
F9
004-159091578F
CCCCAACAGCTGCAAATAACGAAG
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
F10
004-159091578R
ATGCGACTCTTGGCGGACAG
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
G9
014-077327606F
GCCTGGTATAGAACAAGCACCTG
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
G10
014-077327606R
CCTCCTGTGATTGCCAGAGC
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
H9
005-074840050F
TTTAAAGGGCAAGGCAGCCAGG
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
H10
005-074840050R
GCACACCACCATGCCTGGTT
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
A11
008-132042625F
GAGGAAGATCCATACCCTTCCAC
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
A12
008-132042625R
GCCTGCTGGGTTTTATGTCCTAG
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
B11
010-133959783F
TTTCTCTCGGCACCTCCCAC
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
B12
010-133959783R
GTCCCTGCCCATGGACAAGT
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
C11
018-076525272F
TGGTGTCTCCCGTGTGGCTT
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
C12
018-076525272R
CACTTTCCCAAACGTCACGGC
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
D11
007-073021712F
GATGCGCCGCTTCCAGTAGT
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
D12
007-073021712R
TGTGAAGCGGAGGAAGAGCC
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
E11
009-117519299F
CAAAAGGACCTGCATCTAAGCACC
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
E12
009-117519299R
ATTGTTCTTCCCTGGCCACCC
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
F11
009-107888167F
CACTTCTAGGACTCAGTCGTCATG
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
F12
009-107888167R
GTGGGCATGGTAAGAGGTATTGCT
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
G11
015-100263695F
GACTGAGGAGAGACTGCTGTTC
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
G12
015-100263695R
GTGGAGACCTTAAGAGATGTCTCG
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
H11
001-078685858F
GCTGGTCTGCCACAGTGCTT
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
H12
001-078685858R
CCTGCACAAATGTCTCTTGGAACC
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
Primer Plate #3
A1
004-053572737F
CTAACAATGTGTCCATGGGGTTGC
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
A2
004-053572737R
GGGATTACAGGCATGAGCCAC
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
B1
019-022376173F
GGTACTCTCACTGTGGAAAACTGG
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
B2
019-022376173R
GGCCAATTCTCCTCTCCTTTTTCC
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
C1
001-035182032F
ATGCAGAAAATCCGGATGGCCTGG
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
C2
001-035182032R
GGGACCTTGTGATAGAAGCCTG
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
D1
007-141775417F
GGGTGAAGGACCTGAACAGATG
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
D2
007-141775417R
CCCAGAAGTGGGAATTCATGGGA
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
E1
006-128845862F
GAAGGAGGCAAGGCAGGTATG
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
E2
006-128845862R
CGTACCCATGCAAGAAGTTTCCTG
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
F1
007-101606440F
CTCACCTGGCAGGAAAATTCCC
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
F2
007-101606440R
ATGAACTGCTGGGTGCAGCC
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
G1
010-072329199F
CGGCATTTGCAAACCATCCATCTG
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
G2
010-072329199R
TTTTGGTTTTCTGAGGACCCCCC
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
H1
010-045473446F
ATGGGCCACGGAGAGCAGAA
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
H2
010-045473446R
GCCTTCCTCTGACCAGTGTTTG
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
A3
009-134936820F
GCTGGTAAGTGAAGTTGATGTGGC
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
A4
009-134936820R
CCAGGAGCAGCTGCGATGAA
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
B3
017-041000067F
GCTACCATGCCCGGCCTTTT
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
B4
017-041000067R
GCTGTTCTATGGCTGTGCCC
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
C3
003-116802161F
GGGATGGTCCAAAACTCAAACCTC
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
C4
003-116802161R
GAAGGCATTGGGGCTAGACAAG
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
D3
017-034179828F
CCAGCGACTGCTTGGATAAAGTAG
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
D4
017-034179828R
CTGCAAAATCCTGCCCACAGATC
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
E3
017-046203475F
GCAATGCAGCCTGCCTGCTA
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
E4
017-046203475R
CTGAGGCACCTGGAAGGGTT
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
F3
014-026548055F
GGGAGGCTGACTTGAATGAGCT
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
F4
014-026548055R
GTTGCCAGGTGAATAAAAGGAGAGTGAC
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
G3
00X-134006532F
CGCAAAGGTCATCAGTAAGCACAC
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
G4
00X-134006532R
GAAAGAGGAGGCTTAGAGTTGGG
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
H3
004-012977409F
GACACTTGAAGCCTGAGAATCGC
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
H4
004-012977409R
CAACTGTGAGCCTGTGTAGATGG
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
A5
014-055654691F
CCTCTAAGCCTTTGCAAACGGG
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
A6
014-055654691R
GGGACCATTAGGATGGAAAGAACC
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
B5
015-026072103F
GTCAAAAGCCCAAAACCCTACATTCGG
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
B6
015-026072103R
GTCTGCTAGTGGTGACTTCTCTG
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
C5
017-072588236F
CAGAGCGTCTCTGTCTCCAAG
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
C6
017-072588236R
GGCACACCTTGCAACATACACTG
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
D5
001-213135498F
TGGTTTGGTCCCTTTCTTCTCCG
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
D6
001-213135498R
AAGCCAGTCAATGTGCCTGGG
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
E5
006-097126340F
CAGGTGAGGAAACTGAGGCATAC
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
E6
006-097126340R
ATGCGAGGCAGATGAAGAGAGAC
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
F5
006-111761973F
GTAACATTTCTTACTGGCAACAGATTCCCTC
S2_S5_NN_Ranking-RVDs	Warning! Primer pair was generated using suboptimal parameters. 
F6
006-111761973R
GAGGCATAGGGATTGGAATTGTAAGAAAAGG
S2_S5_NN_Ranking-RVDs	Warning! Primer pair was generated using suboptimal parameters. 
G5
006-109827303F
CTGGGGATTCGGTTTCAGCATATG
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
G6
006-109827303R
CAGCAAATGGTGGGTATGGAATGAAGTC
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
H5
017-034950345F
GAATGTCAGCTTCCCCCTGG
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
H6
017-034950345R
GCTCAGAGTTCTCAGACATGCC
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
A7
218-000043242F
CAATCAGGGAGGGTCTCTGG
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
A8
218-000043242R
CTTGGGTGGCCAGGACTTGT
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
B7
012-123425468F
CCGGTCGATGAACTCGAACAC
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
B8
012-123425468R
AGAGTGCTAGTGATGCTGCTGTC
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
C7
002-072161892F
GGCTGCTGTCTTTGATGATGCAAC
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
C8
002-072161892R
GCCCCTGGCAATGAAGGCAA
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
D7
007-051111717F
GCTGGCCTTTGGCTTAATGGAAC
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
D8
007-051111717R
CTATCTACAAATAGCTAGGTAGTTGCGCAG
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
E7
007-047858063F
CCACTTTCGGGGAAATGGTATCAC
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
E8
007-047858063R
GCAAGCGTTCTATCCAAGTGCC
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
F7
006-033661964F
CTGAAGGGGAGCCTGGAAAAAC
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
F8
006-033661964R
TCCTTCCTTCCTTCCTCCCG
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
G7
003-054731501F
CCTACAGTTTCCAAGGACAAGCAG
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
G8
003-054731501R
CCCAGCAGTAGCTTTAGCATCC
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
H7
012-059855477F
GAGAAAGGATTGAGTATTTTTCTCAGGACTTGG
S2_S5_NN_Ranking-RVDs	Warning! Primer pair was generated using suboptimal parameters. 
H8
012-059855477R
CAGAATTTCAGAGGCCCTCCATC
S2_S5_NN_Ranking-RVDs	Warning! Primer pair was generated using suboptimal parameters. 
A9
004-002790263F
ATGCAGAGATGGCCCAGAGC
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
A10
004-002790263R
TGAGTAGGGCCAAGGCCACA
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
B9
001-163952024F
GCAGCCCCTTTCAACTTAGACTAG
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
B10
001-163952024R
CTAGTCCCTGCCCACATTTCC
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
C9
003-089962457F
CCATGACATTGAGAACAGTCGTTTTCCTAG
S2_S5_NN_Ranking-RVDs	Warning! Primer pair was generated using suboptimal parameters. 
C10
003-089962457R
CTAGAGGTGGAAAAAAACAACTAGAGATGGG
S2_S5_NN_Ranking-RVDs	Warning! Primer pair was generated using suboptimal parameters. 
D9
002-130879063F
AAGACGCCCACCGAGCCCA
S2_S5_NN_Ranking-RVDs	Warning! Primer pair was generated using suboptimal parameters. 
D10
002-130879063R
GCTAATGGGTGCAAAGTTTCTTTTAAGGAGCATA
S2_S5_NN_Ranking-RVDs	Warning! Primer pair was generated using suboptimal parameters. 
E9
002-131974522F
GCTAATGGGTGCAAAGTTTCTTTTAAGGAGCATA
S2_S5_NN_Ranking-RVDs	Warning! Primer pair was generated using suboptimal parameters. 
E10
002-131974522R
AAGACGCCCACCGAGCCCA
S2_S5_NN_Ranking-RVDs	Warning! Primer pair was generated using suboptimal parameters. 
F9
013-027482613F
ATCTGGTCAGTGGAGGTTTGGG
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
F10
013-027482613R
CTCACTCTTACTCAGTGCCTGC
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
G9
005-178559029F
AACCCTGGCCTCTCCACAAG
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
G10
005-178559029R
GCCTTCCCTTCTCAGTTTTCTGTG
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
H9
017-042857853F
GTCCCAGATCGTCTCCAATTCG
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
H10
017-042857853R
CAGAGACCCTAACCCTCCAG
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
A11
018-008794462F
AAGAAGGCTCTCCAGGAGGG
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
A12
018-008794462R
GTCTGGTCTCTGGTCTCTTAGTC
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
B11
001-178491197F
GTCTCAGGAGAGACCCTGCT
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
B12
001-178491197R
GAAGGAATGTTCCCTTTGGGGGT
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
C11
009-019935343F
CCTTGCTGCTGAAGAGGATCTG
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
C12
009-019935343R
AGGAACCTACCCTCCTATGCC
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
D11
001-178499007F
CACTTATCATGTCGAGGCCTGC
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
D12
001-178499007R
CCCTCTTAATTGCTCCCAGATGAC
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
E11
002-031143947F
GACAGGAAGGTCAGCTGGGA
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
E12
002-031143947R
GAATCTGTGTGTGGGCAGTGCT
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
F11
010-100332368F
GAGAGTGAACTCACCTTCCATGAC
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
F12
010-100332368R
TGGGCACACAGTCAGTTCCTAG
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
G11
013-029811755F
GGCTTCAGCTTCTACTCTGGG
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
G12
013-029811755R
CCTGTCATGAGGTTGCTGGATC
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
H11
008-002210079F
GATGTCTGGAAGTCCGAGAAGC
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
H12
008-002210079R
GAGGTTTCCCCAAAGTGTCCTTC
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
Primer Plate #4
A1
009-138659749F
CAGGAGAAGCTGTCAGCTGG
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
A2
009-138659749R
CTGGACACCCTAGGAGACTCCAA
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
B1
002-097493184F
TAGTAGAGGCGGGGTTTTGCCA
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
B2
002-097493184R
AAAAAGGGACAGTGTCCACTGGG
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
C1
015-081145141F
GCGTTAAGTGTCTGGAAGGCG
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
C2
015-081145141R
GGAACTCTCAGCCACAACAAACAC
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
D1
004-111129424F
GACCAGTATGGGAAGTCCTGATG
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
D2
004-111129424R
CCGTGGGGTAAATACAGGTCAAC
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
E1
014-093175798F
CTCCCTACTACCGAAGAGGTC
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
E2
014-093175798R
GATCTGGAGGAGTCCAGGCA
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
F1
006-119691851F
CCCCCCCAATATCCTGATTGAAC
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
F2
006-119691851R
CCCAGAAAAGGGATCACTGCATCA
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
G1
001-110268884F
CAGGCAGGCAATCTTGATGTGG
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
G2
001-110268884R
CTCCTTTTTCCTGCGTAGCAAGTG
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
H1
011-102401741F
GACTGCTCTCATAGGTATCATTCAGGAC
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
H2
011-102401741R
GAAGAATTAGAGGCAGTGTTCCCC
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
A3
00X-070076863F
CAGGTGTGGTAAAGTGAACTGGG
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
A4
00X-070076863R
GACACTAGTGGCACTTGAAGGAG
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
B3
008-059835610F
GGGATCTTGGCAGAGCCTGA
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
B4
008-059835610R
CAAGTCTCCACAGAAATGCCTCC
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
C3
004-111619023F
GGCAGGCATCTCTTCAACACAC
S2_S5_NN_Ranking-RVDs	Warning! Primer pair was generated using suboptimal parameters. 
C4
004-111619023R
CCCAATTAAGAAATGGGCAAAGGATCTAAATAGAC
S2_S5_NN_Ranking-RVDs	Warning! Primer pair was generated using suboptimal parameters. 
D3
019-005861521F
ACAGCTGTCCTGTGGATGTGAAG
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
D4
019-005861521R
CAGTGTGGCCTGAGCTAGGA
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
E3
001-109847447F
GCAGGCTTGCCAAACTAAAAGCTC
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
E4
001-109847447R
TCAGAGAGCCAACAGAGCCC
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
F3
020-050528903F
TTGCTTGAACCCAGGAGGCG
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
F4
020-050528903R
GGGTTGGATGGGAAAGCAGAG
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
G3
004-118702135F
GCAGGGACACTGGTGTTATGTCT
S2_S5_NN_Ranking-RVDs	Warning! Primer pair was generated using suboptimal parameters. 
G4
004-118702135R
GATAGTAACTTATTTAGAATTGAGGTTGTGTGATGGAG
S2_S5_NN_Ranking-RVDs	Warning! Primer pair was generated using suboptimal parameters. 
H3
003-060011649F
GTACTGCCAGAGTGTCAAGAAAGC
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
H4
003-060011649R
TAGGAAGAGGACAGTAGGCTAGG
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
A5
005-044679170F
TCCTTACTAGCCCCCATTAACTCC
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
A6
005-044679170R
AATGTCTCCTAGAGGAGCATGGC
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
B5
017-075205760F
CTGTGCTTGATCAGAGTGCCTGG
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
B6
017-075205760R
TTGCTTGAACCCATAAGGCGGAG
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
C5
005-157749065F
GCATGGAAGAGCATGGTAGGG
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
C6
005-157749065R
GTCAGGTATACTAGGTGCTGTGG
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
D5
009-126860975F
TGTGCGTGAGTCATTACCAGGAAC
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
D6
009-126860975R
CCCTGTGGTGAGCATGAAGC
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
E5
002-062470613F
CAATCAGGAATGAAAGAGGTGACTTCCTTAG
S2_S5_NN_Ranking-RVDs	Warning! Primer pair was generated using suboptimal parameters. 
E6
002-062470613R
GGTGGGAGTTCACATAGGAGG
S2_S5_NN_Ranking-RVDs	Warning! Primer pair was generated using suboptimal parameters. 
F5
003-138953648F
CTTGGTGTCTGAGTGGCAAAACTG
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
F6
003-138953648R
TATGGGCCTGTTTTACACGGGCCT
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
G5
022-047592728F
AATGAGGCCTGGGAGCAGGA
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
G6
022-047592728R
CACCAAGAGCACCCCAAAGG
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
H5
010-124341992F
GCCCTGGTGACTTTGGCATTTG
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
H6
010-124341992R
ATTTCCTGGGGCTGACGTGG
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
A7
010-124348357F
CCAGGTGACTTTGGCCATTAGG
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
A8
010-124348357R
ATTTCCTGGGGCTGACGTGG
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
B7
010-124354695F
GCCCTGGTGACTTTGGCATTTG
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
B8
010-124354695R
ATTTCCTGGGGCTGACGTGG
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
C7
001-054971975F
GGACCATAATCTCCCCTACAGC
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
C8
001-054971975R
GCTGAATTCTGCTACTCCTTGGC
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
D7
007-066395352F
AGAGGCCCCAGCTGGAACTT
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
D8
007-066395352R
CAAAGCAGTGCAGGCAGAATGAAC
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
E7
016-007614298F
TTGGGACGCAGAGGTGGGTA
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
E8
016-007614298R
CTGGCTTGCAGATGAGTGCCT
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
F7
004-067641777F
CTGGTTTAATGCTCTGCTGTTGCC
S2_S5_NN_Ranking-RVDs	Warning! Primer pair was generated using suboptimal parameters. 
F8
004-067641777R
CAGGAATACAGCCATTTGACATTTTCACTGAAAAG
S2_S5_NN_Ranking-RVDs	Warning! Primer pair was generated using suboptimal parameters. 
G7
012-062445219F
GAGAGGATACAAGGTCAGGTATGG
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
G8
012-062445219R
GGCCCATTTTAGAAAACATTTGCTGACTCC
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
H7
010-081345161F
GAGAAAGGTCTTTGCTAACCCGG
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
H8
010-081345161R
GGCTGATTTGAGGACACACTGC
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
A9
020-003747479F
GAGCAGAAAAATGCCCTACCTCC
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
A10
020-003747479R
CCCCCTGAGATGACAAGAATGG
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
B9
006-110679146F
TTTGCAACTGACCTTCCGGCG
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
B10
006-110679146R
GCTGTGCTGTTGTTCGGCCT
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
C9
005-169132663F
CCAGCCTGATTCCTACCTTAGGCA
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
C10
005-169132663R
ATAGGTGTGGGAGACTTCAGGTCA
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
D9
00X-136731121F
GTCACTTTACCTCCCTGAACCTC
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
D10
00X-136731121R
AGACAGCATTTCGCCATGTTGACC
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
E9
022-017471581F
GGGGTCTATCAGCACCAAGC
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
E10
022-017471581R
GGGCTCAGCAGATACTTCCTGT
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
F9
014-024242484F
CAAGTCTTCCTCAGCCATCAGACA
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
F10
014-024242484R
CCAGACACATCAACAGAGTGGAAG
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
G9
011-011275773F
TCCACTGTGGCCAGAGCTAC
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
G10
011-011275773R
GTACCTGGAAGCCCCTCTGA
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
H9
001-153175860F
CTCATAAGTGGAGGTCATTTCCCC
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
H10
001-153175860R
GCACACACCAGTTCTTACCACTGA
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
A11
001-030487647F
CCATTGTCCTGAGGATGAACTGTC
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
A12
001-030487647R
TCTGCACCCATGGGCTGAGT
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
B11
014-046262053F
GTCTTTACCCTCCACCCTTGTTTC
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
B12
014-046262053R
GGAGCTTGAGTTTTCAGGCTGTTC
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
C11
00X-070322882F
CCTGGAATTAGCTTGGGGCC
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
C12
00X-070322882R
TGATCCACATCCCCCCCCAT
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
D11
003-126821831F
CCTGCATTCAGGAAACTTCCGTTG
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
D12
003-126821831R
GCTGATGGCTTCTGCCATTCTTG
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
E11
004-038097102F
GCTACCAAATGGAGGTTTGGCTCT
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
E12
004-038097102R
CCATGCCTCTACCCATTTTCCTC
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
F11
003-015967056F
CAGGCCATGGTAAGAGGATAGG
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
F12
003-015967056R
CCAAACTCCAAGCCCACACAC
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
G11
014-075136937F
GAGGATCAGAGCCCATCCAAATGT
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
G12
014-075136937R
GGAACCTGACAACCTGTGTCCT
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
H11
009-017690436F
CCCCACATCACATGTCCTCTTTAG
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
H12
009-017690436R
CCTAGTACCCGGCACTTTTCATG
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
Primer Plate #5
A1
00X-148727617F
GAGTCTTCCATAGCTGAGTGCAAC
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
A2
00X-148727617R
ACCCAACATCTGCCATCTGCAAG
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
B1
016-034421445F
GGTGGAAGCAAATATGGGGAGG
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
B2
016-034421445R
GGCAGAAATGGTTCCCAGGAAAAG
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
C1
022-029629152F
ATTGCAGTGGGGTTAGAACCCACA
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
C2
022-029629152R
TGGGGCCAGTGGTTGTTGGT
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
D1
009-039525524F
GCCCTGTGGATGCCACATTC
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
D2
009-039525524R
CTGGAAGGGGCATGGAATATGAG
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
E1
009-040853051F
GCCCTGTGGATGCCACATTC
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
E2
009-040853051R
CTGGAAGGGGCATGGAATATGAG
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
F1
009-041229954F
GGATTTAATCCACAGGCAAGGAGG
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
F2
009-041229954R
GCCCTGTGGATGCCACATTC
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
G1
009-041670537F
GCCCTGTGGATGCCACATTC
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
G2
009-041670537R
CTGGAAGGGGCATGGAATATGAG
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
H1
009-043533250F
GGATTTAATCCACAGGCAAGGAGG
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters. 
H2
009-043533250R
GCCCTGTGGATGCCACATTC
S2_S5_NN_Ranking-RVDs	Primer pair generated using optimal parameters.