Top Cleavage Sites for S2_S5_NN_Ranking-Homology

PROGNOS Summary for S2_S5_NN_Ranking-Homology_5T

The hg19 genome was searched for the TALEN pair targeting:
5'- TCACCTTGCCCCACAGGGCAGT NN..NN ACAGGAGTCAGGTGCA -3'

Up to 5 mismatches were allowed in each nuclease half-site.

Allowed spacing distances were: 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30

Homodimers and heterodimers of the nucleases were included in the output

3481 total sites were located
Mismatch Types Genomic Regions
  0 and 2: 1
  2 and 2: 1
  3 and 3: 3
  2 and 5: 8
  3 and 4: 18
  3 and 5: 112
  4 and 4: 63
  4 and 5: 813
  5 and 5: 2462
  Exons: 143
  Promoters: 28
  Introns: 1383
  Intergenic: 1927


The top 200 sites are ranked below according to a weighted sequence homology. All sites listed contain flanking 5' thymidines.
For the full output, click here.

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Primer pairs were designed using the 400 bp of sequence on either side of the cleavage site to melt close to 60 °C

For easy visualization of cleavage on gels:
The smallest fragment will be longer than 130 bp, which is also the minimum separation distance between uncleaved and cleaved products.
The two cleavage products will be separated by a minimum size of 0 bp
To optimize assay conditions, the amplicons have a minimum length of 260 bp, a maximum length of 450 bp, and an optimal length of 270 bp.
Rank: 1	Homology Score: 52
	Genomic Region: Exon	Closest Gene: HBB	Caution! This site scores very closely to the intended nuclease target. The potential for cleavage at this site is of great concern.
Match Type: L16R
	           Left Nuclease
	         TCACCTTGCCCCACAGGGCAGT
	5'-CACGT TCACCTTGCCCCACAGGGCAGT        AACGGCAGACTTCTCC              TCAGGAGTCAGATGCA CCATG-3'
	3'-GTGCA AGTGGAACGGGGTGTCCCGTCA        TTGCCGTCTGAAGAGG              AGTCCTCAGTCTACGT GGTAC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 011-005248069
Forward Primer: 5'-GTGCCTATCAGAAACCCAAGAGTC-3' T_m: 59.038
Reverse Primer: 5'-CAATCTACTCCCAGGAGCAGG-3' T_m: 59.378
Expected PCR Product Length: 308
Expected CelI Cleavage Product Sizes: 154, 154
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr11:5248194-5248247

Rank: 2	Homology Score: 32
	Genomic Region: Exon	Closest Gene: HBD	Caution! This site scores very closely to the intended nuclease target. The potential for cleavage at this site is of great concern.
Match Type: L16R
	           Left Nuclease
	         TCACCTTGCCCCACAGGGCAGT
	5'-CACGT TCACTTTGCCCCACAGGGCATT        GACAGCAGTCTTCTCC              TCAGGAGTCAGATGCA CCATG-3'
	3'-GTGCA AGTGAAACGGGGTGTCCCGTAA        CTGTCGTCAGAAGAGG              AGTCCTCAGTCTACGT GGTAC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 011-005255477
Forward Primer: 5'-GTCAGTGCCTATCAGAAACCCAAG-3' T_m: 59.038
Reverse Primer: 5'-TCACTGGAGCAGGGAGGACA-3' T_m: 59.508
Expected PCR Product Length: 296
Expected CelI Cleavage Product Sizes: 158, 138
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr11:5255606-5255659

Rank: 3	Homology Score: 18
	Genomic Region: Intron	Closest Gene: PHF21B
Match Type: R12R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CATTC TCCACCTGTCTCCTGC                CTCTCAACTGGG                TCTGGAGTCAGGTGGA AAAAT-3'
	3'-GTAAG AGGTGGACAGAGGACG                GAGAGTTGACCC                AGACCTCAGTCCACCT TTTTA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 022-045287459
Forward Primer: 5'-CAAAGGCTCTGCCTGCAGGA-3' T_m: 59.508
Reverse Primer: 5'-CAGGACTAAGGCCGCTCTTC-3' T_m: 59.508
Expected PCR Product Length: 269
Expected CelI Cleavage Product Sizes: 136, 133
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr22:45287571-45287614

Rank: 4	Homology Score: 18
	Genomic Region: Intron	Closest Gene: FAM3D
Match Type: R17R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CCCTG TGCCCCTGACTCCTTA             CAGCTGTTCTAGAATGT              AAATGAGGCAGGTGCA TTTCG-3'
	3'-GGGAC ACGGGGACTGAGGAAT             GTCGACAAGATCTTACA              TTTACTCCGTCCACGT AAAGC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 003-058640715
Forward Primer: 5'-CATATGAGGAAACTGAGAGGCGG-3' T_m: 59.148
Reverse Primer: 5'-CCTCGAGGAGGGCTATTCTAGA-3' T_m: 59.258
Expected PCR Product Length: 283
Expected CelI Cleavage Product Sizes: 130, 153
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr3:58640819-58640867

Rank: 5	Homology Score: 18
	Genomic Region: Intron	Closest Gene: PDE4D
Match Type: R29R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CACAC TGCACCTAACATCTGT       CCTTATAATAAACTAGGAGCATCTCTGTT        ACAGGAATCATGTTCA GTAGT-3'
	3'-GTGTG ACGTGGATTGTAGACA       GGAATATTATTTGATCCTCGTAGAGACAA        TGTCCTTAGTACAAGT CATCA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 005-059619945
Forward Primer: 5'-CCAAGACTTAGCTTAGCACCCAAC-3' T_m: 59.038
Reverse Primer: 5'-CATGCCTGGAGTTAGCCTCTCT-3' T_m: 59.258
Expected PCR Product Length: 384
Expected CelI Cleavage Product Sizes: 219, 165
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr5:59620132-59620192

Rank: 6	Homology Score: 17
	Genomic Region: Intron	Closest Gene: CABIN1
Match Type: R10R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GGGGT TGCATCTGAATCCTGT                 GGGTGTGGCA                 GCAGGGGACAGCTGGA AAAGG-3'
	3'-CCCCA ACGTAGACTTAGGACA                 CCCACACCGT                 CGTCCCCTGTCGACCT TTTCC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 022-024555541
Forward Primer: 5'-CACAGCCATTCTGTCTACTCCC-3' T_m: 59.258
Reverse Primer: 5'-TGGCAGGTGGCCAGTGTCTT-3' T_m: 59.508
Expected PCR Product Length: 273
Expected CelI Cleavage Product Sizes: 140, 133
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr22:24555658-24555699

Rank: 7	Homology Score: 17
	Genomic Region: Intron	Closest Gene: CARD11
Match Type: R17R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GGGGG TGCACCTGCCTCCTGC             CTGCACCCTGGGAGCTT              GCAGGATGCAGAAGCA TGGGC-3'
	3'-CCCCC ACGTGGACGGAGGACG             GACGTGGGACCCTCGAA              CGTCCTACGTCTTCGT ACCCG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 007-002962408
Forward Primer: 5'-ACAGCGTTTGTCTCCTGGCC-3' T_m: 59.508
Reverse Primer: 5'-GCCCCTAAGCCTTGCATTCAG-3' T_m: 59.378
Expected PCR Product Length: 298
Expected CelI Cleavage Product Sizes: 167, 131
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr7:2962549-2962597

Rank: 8	Homology Score: 17
	Genomic Region: Intron	Closest Gene: SDK1
Match Type: R13R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TGTTT TGAACCTGACTCCTTT               CACATGGCATAGC                ACATGTGACACATGCA TATCA-3'
	3'-ACAAA ACTTGGACTGAGGAAA               GTGTACCGTATCG                TGTACACTGTGTACGT ATAGT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 007-003795640
Forward Primer: 5'-CCTGACTCCTTTCACATGGCATAG-3' T_m: 59.038
Reverse Primer: 5'-TGTGTCACACGTGCTATGCCATG-3' T_m: 59.148
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 135, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr7:3795751-3795795

Rank: 9	Homology Score: 17
	Genomic Region: Intron	Closest Gene: SDK1
Match Type: R13R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TGTTT TGAACCTGACTCCTTT               CACATGGCATAGC                ACATGTGACACATGCA TATCA-3'
	3'-ACAAA ACTTGGACTGAGGAAA               GTGTACCGTATCG                TGTACACTGTGTACGT ATAGT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 007-003795876
Forward Primer: 5'-GAGTCCTTTCACATGGCATAGCAC-3' T_m: 59.038
Reverse Primer: 5'-ACATGTCTCACACGCACAATGCC-3' T_m: 59.148
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 135, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr7:3795987-3796031

Rank: 10	Homology Score: 17
	Genomic Region: Intergenic	Closest Gene: GRIK2
Match Type: R29R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CTTGG TGCACCCGACTGCTGT       GGGGTGTCCCAGCAGGAGGTTGTCAATGT        ACTGGAGCAACATGCA ACCTA-3'
	3'-GAACC ACGTGGGCTGACGACA       CCCCACAGGGTCGTCCTCCAACAGTTACA        TGACCTCGTTGTACGT TGGAT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 006-101676033
Forward Primer: 5'-CCAGGTAACATACTTGCTGTCTGC-3' T_m: 59.038
Reverse Primer: 5'-GTCACTACTCAGTACACTTGGACC-3' T_m: 59.038
Expected PCR Product Length: 275
Expected CelI Cleavage Product Sizes: 131, 144
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr6:101676132-101676192

Rank: 11	Homology Score: 17
	Genomic Region: Intergenic	Closest Gene: GPR6
Match Type: R30R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CCATC TCCACCTGGCTCCTGT       GTGGTCCATGTTGCAAGTTTTCAGGAAGGA       GCAGGAGTTAAGGGTA GAGTT-3'
	3'-GGTAG AGGTGGACCGAGGACA       CACCAGGTACAACGTTCAAAAGTCCTTCCT       CGTCCTCAATTCCCAT CTCAA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 006-110284189
Forward Primer: 5'-GCAATGTTGCTCCAAGAGGCC-3' T_m: 59.378
Reverse Primer: 5'-GAACTTATTCCTCCCACCTAGCTG-3' T_m: 59.038
Expected PCR Product Length: 297
Expected CelI Cleavage Product Sizes: 131, 166
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr6:110284287-110284348

Rank: 12	Homology Score: 17
	Genomic Region: Intergenic	Closest Gene: SH3BP4
Match Type: R23R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GCAAT TGCACCTCACTTCTGT          GGAGTGCTTCCTGTGTATCAGGC           ACAGAAGCCCGATGTA TTCTC-3'
	3'-CGTTA ACGTGGAGTGAAGACA          CCTCACGAAGGACACATAGTCCG           TGTCTTCGGGCTACAT AAGAG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 002-235805535
Forward Primer: 5'-ACCTCCACCAGACCACGAATG-3' T_m: 59.378
Reverse Primer: 5'-GACCCTTAGCAGGTCACACTC-3' T_m: 59.378
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 135, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr2:235805641-235805695

Rank: 13	Homology Score: 17
	Genomic Region: Intergenic	Closest Gene: SLC2A10
Match Type: R24R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CCCCT TCCTCCAGCTTCCTGT          AGGTTCAACTAGGGGGAAGCCCCA          GCAGGAGACAGGTGCA CAGTG-3'
	3'-GGGGA AGGAGGTCGAAGGACA          TCCAAGTTGATCCCCCTTCGGGGT          CGTCCTCTGTCCACGT GTCAC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 020-045428486
Forward Primer: 5'-GTCTCACTGAGACCTAGGCG-3' T_m: 59.508
Reverse Primer: 5'-TGGGCCTGGGGAACCAACAA-3' T_m: 59.508
Expected PCR Product Length: 268
Expected CelI Cleavage Product Sizes: 133, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr20:45428589-45428644

Rank: 14	Homology Score: 13
	Genomic Region: Intron	Closest Gene: RALGPS1
Match Type: R22R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TGAGC TGCGCCTGAGTCCAGG           CCTTGCTATTCCTGAGATAGAA           AACGGAGTCAGATGCA GAGCT-3'
	3'-ACTCG ACGCGGACTCAGGTCC           GGAACGATAAGGACTCTATCTT           TTGCCTCAGTCTACGT CTCGA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 009-129960176
Forward Primer: 5'-CTTCCTTGGAGTGTCCAATGGG-3' T_m: 59.258
Reverse Primer: 5'-CATCAGGGCTTTTGGCCTCAAAC-3' T_m: 59.148
Expected PCR Product Length: 278
Expected CelI Cleavage Product Sizes: 148, 130
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr9:129960295-129960348

Rank: 15	Homology Score: 13
	Genomic Region: Intron	Closest Gene: GRIA2
Match Type: R19R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-ATCCA TACACCTGTCTGCTGT            AACCTTTTCCAGATTGGCT             ACTGGAGTGAAGTGGA CAAAA-3'
	3'-TAGGT ATGTGGACAGACGACA            TTGGAAAAGGTCTAACCGA             TGACCTCACTTCACCT GTTTT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
No Primers
Primer Index: No Primers
Forward Primer: 5'-No Primers-3' T_m: No Primers
Reverse Primer: 5'-No Primers-3' T_m: No Primers
Expected PCR Product Length: No Primers
Expected CelI Cleavage Product Sizes: No Primers, No Primers
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr4:158255134-158255184

Rank: 16	Homology Score: 13
	Genomic Region: Intron	Closest Gene: ERGIC1
Match Type: R10R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-AGGTC TGCTCCTGCCTCCAGT                 AGAAGCAGCC                 ACAGGAGTCCTGGCCA CAGCC-3'
	3'-TCCAG ACGAGGACGGAGGTCA                 TCTTCGTCGG                 TGTCCTCAGGACCGGT GTCGG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 005-172280139
Forward Primer: 5'-TGGTTTACCACGTGCTGTTGTTGC-3' T_m: 59.038
Reverse Primer: 5'-AAGATGCCTCTGGGTGGCCT-3' T_m: 59.508
Expected PCR Product Length: 272
Expected CelI Cleavage Product Sizes: 131, 141
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr5:172280247-172280288

Rank: 17	Homology Score: 13
	Genomic Region: Intron	Closest Gene: SORBS2
Match Type: R12R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-AATGT TTTTCCTGACTTCTGT                GATGTATAGGGC                ACTGTAGTCAGGTGAA ATAAC-3'
	3'-TTACA AAAAGGACTGAAGACA                CTACATATCCCG                TGACATCAGTCCACTT TATTG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 004-186747672
Forward Primer: 5'-CTGTGAGCTACTGAGGATATGCG-3' T_m: 59.148
Reverse Primer: 5'-GGGCAGATGCTTAAATCACTCCCA-3' T_m: 59.038
Expected PCR Product Length: 374
Expected CelI Cleavage Product Sizes: 218, 156
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr4:186747866-186747909

Rank: 18	Homology Score: 13
	Genomic Region: Intron	Closest Gene: SCRN1
Match Type: R10R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GTCAC TGTACCTGACTGATGT                 ATACTTTTTA                 TCATAAGCCAGGTGCA GTGGC-3'
	3'-CAGTG ACATGGACTGACTACA                 TATGAAAAAT                 AGTATTCGGTCCACGT CACCG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 007-030006854
Forward Primer: 5'-CCAAGAAGGTTATGCCCTGAATGC-3' T_m: 59.038
Reverse Primer: 5'-TAGTGGGTGTCCTTGGCTCTG-3' T_m: 59.378
Expected PCR Product Length: 433
Expected CelI Cleavage Product Sizes: 303, 130
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr7:30007134-30007175

Rank: 19	Homology Score: 13
	Genomic Region: Intron	Closest Gene: PBRM1
Match Type: R22R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TCTCC TGCAACTGAAGCCTGT           ATTTAATTTAAGCAGGAATGGC           ACTGGAATCAGGCTCA GCAGT-3'
	3'-AGAGG ACGTTGACTTCGGACA           TAAATTAAATTCGTCCTTACCG           TGACCTTAGTCCGAGT CGTCA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 003-052584959
Forward Primer: 5'-GGCTAGGAATCTTAAGCACCCC-3' T_m: 59.258
Reverse Primer: 5'-GAGGGGATAGCAAGGCTGTG-3' T_m: 59.508
Expected PCR Product Length: 378
Expected CelI Cleavage Product Sizes: 246, 132
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr3:52585176-52585229

Rank: 20	Homology Score: 13
	Genomic Region: Intron	Closest Gene: CACNA2D3
Match Type: R11R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CAAGG TGCCCCACACTCCTGT                GGTCTGGAAGG                 AATGGAGCCATGTGCA CACTT-3'
	3'-GTTCC ACGGGGTGTGAGGACA                CCAGACCTTCC                 TTACCTCGGTACACGT GTGAA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 003-054731501
Forward Primer: 5'-CCTACAGTTTCCAAGGACAAGCAG-3' T_m: 59.038
Reverse Primer: 5'-CCCAGCAGTAGCTTTAGCATCC-3' T_m: 59.258
Expected PCR Product Length: 320
Expected CelI Cleavage Product Sizes: 160, 160
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr3:54731638-54731680

Rank: 21	Homology Score: 13
	Genomic Region: Intron	Closest Gene: HYDIN
Match Type: R26R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CCCTC TGCACACAACTCCTAT         GCACTCTGCACGCACCACCCTCCTGC         ACAGGCGTCCCGTGCA GGCTG-3'
	3'-GGGAG ACGTGTGTTGAGGATA         CGTGAGACGTGCGTGGTGGGAGGACG         TGTCCGCAGGGCACGT CCGAC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 016-071012605
Forward Primer: 5'-CTCCTAGCTTCCTGATTCTTTGCC-3' T_m: 59.038
Reverse Primer: 5'-CCCACCCTGGTACAGCAAATG-3' T_m: 59.378
Expected PCR Product Length: 355
Expected CelI Cleavage Product Sizes: 168, 187
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr16:71012742-71012799

Rank: 22	Homology Score: 13
	Genomic Region: Intron	Closest Gene: C14orf166B
Match Type: R24R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TAACT TGCCCCCGACTCATGT          TTTCTTAGATTATCTCTGGCAATC          ACAGGAGGCAGAGGTA AAGCT-3'
	3'-ATTGA ACGGGGGCTGAGTACA          AAAGAATCTAATAGAGACCGTTAG          TGTCCTCCGTCTCCAT TTCGA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 014-077327606
Forward Primer: 5'-GCCTGGTATAGAACAAGCACCTG-3' T_m: 59.148
Reverse Primer: 5'-CCTCCTGTGATTGCCAGAGC-3' T_m: 59.508
Expected PCR Product Length: 368
Expected CelI Cleavage Product Sizes: 179, 189
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr14:77327755-77327810

Rank: 23	Homology Score: 13
	Genomic Region: Intron	Closest Gene: ACSS3
Match Type: R12R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TTGCA TGCACCCTAGTCCTGT                TGTGTGTTAATA                ACAGGCATCAATTGCA TTTTC-3'
	3'-AACGT ACGTGGGATCAGGACA                ACACACAATTAT                TGTCCGTAGTTAACGT AAAAG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Warning! Primer pair was generated using suboptimal parameters. 
Primer Index: 012-081520008
Forward Primer: 5'-GGCAGCAACTCTCCAGTGAGTA-3' T_m: 59.258
Reverse Primer: 5'-CCTGTATTTCCCTATGTATATGTGCTCAGTC-3' T_m: 59.958
Expected PCR Product Length: 413
Expected CelI Cleavage Product Sizes: 171, 242
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr12:81520155-81520198

Rank: 24	Homology Score: 13
	Genomic Region: Intergenic	Closest Gene: SLC25A47
Match Type: R28R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CCTGC TGCAGCTGGCTGTTGT        CCCCGTTCACAGATAGGGAAACCGAGGC        ACAGGAGTTAAGTCCA TTGGC-3'
	3'-GGACG ACGTCGACCGACAACA        GGGGCAAGTGTCTATCCCTTTGGCTCCG        TGTCCTCAATTCAGGT AACCG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 014-100782711
Forward Primer: 5'-TGAAGGGACAGAGCCAGGGT-3' T_m: 59.508
Reverse Primer: 5'-ACACCAGCCTGGGCAACAGA-3' T_m: 59.508
Expected PCR Product Length: 286
Expected CelI Cleavage Product Sizes: 156, 130
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr14:100782835-100782894

Rank: 25	Homology Score: 13
	Genomic Region: Intergenic	Closest Gene: CTSB
Match Type: R29R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CTTGG TGCACCTGACTGCCAT       GGGGTGTCCCAGCAAAAGGGCATCAATCT        ACTGGAGCCACATGCA GTCTA-3'
	3'-GAACC ACGTGGACTGACGGTA       CCCCACAGGGTCGTTTTCCCGTAGTTAGA        TGACCTCGGTGTACGT CAGAT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 008-011765812
Forward Primer: 5'-ATCCCCAGTAATGTACCTGCTGTC-3' T_m: 59.038
Reverse Primer: 5'-CTCAGTACACTTGCACCCGG-3' T_m: 59.508
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 134, 136
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr8:11765914-11765974

Rank: 26	Homology Score: 13
	Genomic Region: Intergenic	Closest Gene: FRG1
Match Type: R14R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GTTTC TACCCCTGACCCCTGC               AGTCTACTCTGAGC               ACAGCAGTCAGAAGCA TTCTT-3'
	3'-CAAAG ATGGGGACTGGGGACG               TCAGATGAGACTCG               TGTCGTCAGTCTTCGT AAGAA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 004-190659833
Forward Primer: 5'-CTGCCTCCAATCCCTCAGAAAATG-3' T_m: 59.038
Reverse Primer: 5'-CTGGTATTGGGTCCAGACCAC-3' T_m: 59.378
Expected PCR Product Length: 308
Expected CelI Cleavage Product Sizes: 178, 130
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr4:190659986-190660031

Rank: 27	Homology Score: 13
	Genomic Region: Intergenic	Closest Gene: HYDIN2
Match Type: R26R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CCCTC TGCACACAACTCCTAT         GCACTCTGCACGCACCACCCTCCTGC         ACAGGTGTCCCGTGCA GGCTG-3'
	3'-GGGAG ACGTGTGTTGAGGATA         CGTGAGACGTGCGTGGTGGGAGGACG         TGTCCACAGGGCACGT CCGAC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: dom-000216374
Forward Primer: 5'-TCTCCTTTCTGTACCTCAGAGACC-3' T_m: 59.038
Reverse Primer: 5'-CCCACCCTGGTACAGCAAATG-3' T_m: 59.378
Expected PCR Product Length: 318
Expected CelI Cleavage Product Sizes: 131, 187
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr1_gl000192_random:216474-216531

Rank: 28	Homology Score: 13
	Genomic Region: Intergenic	Closest Gene: GPC1
Match Type: R29R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GAGCC TTCACCTGATTCCAGC       CGCAAAGCCCTTTCCAGTACGGCCCATTC        ACAGGTTCCAGGTGCA AAGCC-3'
	3'-CTCGG AAGTGGACTAAGGTCG       GCGTTTCGGGAAAGGTCATGCCGGGTAAG        TGTCCAAGGTCCACGT TTCGG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 002-241334168
Forward Primer: 5'-CATGCACCCCTGGCTTGTGT-3' T_m: 59.508
Reverse Primer: 5'-AGCTTTGCACCTGGAACCTGTGA-3' T_m: 59.148
Expected PCR Product Length: 297
Expected CelI Cleavage Product Sizes: 138, 159
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr2:241334274-241334334

Rank: 29	Homology Score: 13
	Genomic Region: Intergenic	Closest Gene: ATP8B1
Match Type: R11R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-AGGGC TGCACGTGACTTCTGG                GGCAAAGGTAA                 GTAGGAGAAAGGTGCA CCTTC-3'
	3'-TCCCG ACGTGCACTGAAGACC                CCGTTTCCATT                 CATCCTCTTTCCACGT GGAAG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 018-055555293
Forward Primer: 5'-TCTGGGAAGAAGCTGCCAGG-3' T_m: 59.508
Reverse Primer: 5'-GGCTATGCTACCTGCTGTAAAACC-3' T_m: 59.038
Expected PCR Product Length: 342
Expected CelI Cleavage Product Sizes: 134, 208
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr18:55555404-55555446

Rank: 30	Homology Score: 13
	Genomic Region: Intergenic	Closest Gene: FAM69C
Match Type: R26R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CCTAG TTCACTTCACTCCTGT         TATCCACTAAGCTCATTATCACATCG         CCAGAAGTGAGGTCCA TTTGT-3'
	3'-GGATC AAGTGAAGTGAGGACA         ATAGGTGATTCGAGTAATAGTGTAGC         GGTCTTCACTCCAGGT AAACA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 018-072086920
Forward Primer: 5'-GCTGTCACCAGGAAAAACATAAGAGCC-3' T_m: 60.438
Reverse Primer: 5'-AAGCTGCTGAAATGGGCAGAGG-3' T_m: 59.258
Expected PCR Product Length: 289
Expected CelI Cleavage Product Sizes: 159, 130
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr18:72087048-72087105

Rank: 31	Homology Score: 13
	Genomic Region: Intergenic	Closest Gene: ZNF812
Match Type: R29R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TTTTG TGCACCCAACTGCTGT       GGAGTGTCCCAGCAGAACGTCGTCCATGT        ACAGGAGCAAGACGCA GCCTA-3'
	3'-AAAAC ACGTGGGTTGACGACA       CCTCACAGGGTCGTCTTGCAGCAGGTACA        TGTCCTCGTTCTGCGT CGGAT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 019-009837811
Forward Primer: 5'-AATCCCAGGTAGCGTACGTGC-3' T_m: 59.378
Reverse Primer: 5'-TCAGTACACATGGACCTGGCTTC-3' T_m: 59.148
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 135, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr19:9837914-9837974

Rank: 32	Homology Score: 10
	Genomic Region: Exon	Closest Gene: KIF20A
Match Type: R14R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CATCT TACACCCCTCTCCTTT               GGCCCCAGATCTCA               ACTGGATTCATGTGCA AGATG-3'
	3'-GTAGA ATGTGGGGAGAGGAAA               CCGGGGTCTAGAGT               TGACCTAAGTACACGT TCTAC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 005-137519020
Forward Primer: 5'-GCGAGGATCAAAATGGCAATCCC-3' T_m: 59.148
Reverse Primer: 5'-GGCAGAGGACTAATGTTTCCTGG-3' T_m: 59.148
Expected PCR Product Length: 310
Expected CelI Cleavage Product Sizes: 140, 170
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr5:137519135-137519180

Rank: 33	Homology Score: 10
	Genomic Region: Exon	Closest Gene: KIAA1244
Match Type: R19R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CATTG TGTAGCCGACTCCTGT            TCTACTCTCCCACCAAATA             ACAGTAGTGAGGGTTA GAGTC-3'
	3'-GTAAC ACATCGGCTGAGGACA            AGATGAGAGGGTGGTTTAT             TGTCATCACTCCCAAT CTCAG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 006-138657515
Forward Primer: 5'-GTTCCCGTGCATCAGTCAGC-3' T_m: 59.508
Reverse Primer: 5'-GCTGATCAGTGGTTGGAAAGGC-3' T_m: 59.258
Expected PCR Product Length: 272
Expected CelI Cleavage Product Sizes: 131, 141
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr6:138657619-138657669

Rank: 34	Homology Score: 10
	Genomic Region: Exon	Closest Gene: NOL6
Match Type: R28R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-ACCGT TGCACCTCACCTCTGT        GTAGCGCAGCACTGGGTGAGCTCCCTGA        ACAGCAGACACGGTCA GTGGG-3'
	3'-TGGCA ACGTGGAGTGGAGACA        CATCGCGTCGTGACCCACTCGAGGGACT        TGTCGTCTGTGCCAGT CACCC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 009-033466447
Forward Primer: 5'-AATGGGCCTAGGACTGGGAG-3' T_m: 59.508
Reverse Primer: 5'-TCTCCCTCTCAGACCTCCAG-3' T_m: 59.508
Expected PCR Product Length: 273
Expected CelI Cleavage Product Sizes: 142, 131
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr9:33466557-33466616

Rank: 35	Homology Score: 10
	Genomic Region: Exon	Closest Gene: DCHS1
Match Type: R23R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GCTCC TGCACCTGGCAGCAGC          AGCCCTGCCTTTCGGCCCTTATA           CCAGGTGTCAGGGGCA GGTGG-3'
	3'-CGAGG ACGTGGACCGTCGTCG          TCGGGACGGAAAGCCGGGAATAT           GGTCCACAGTCCCCGT CCACC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 011-006643499
Forward Primer: 5'-TGGGAAGCCATAGTCCCCAG-3' T_m: 59.508
Reverse Primer: 5'-ATCAATGAGTTCCCCCGTGTGG-3' T_m: 59.258
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 131, 139
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr11:6643601-6643655

Rank: 36	Homology Score: 10
	Genomic Region: Intron	Closest Gene: NPAS2
Match Type: R14R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GGTTG TGCACCTGTCTCCAGA               CTCTAAGCCTGGGC               ACAGGAGGGGACTGCA CTGAC-3'
	3'-CCAAC ACGTGGACAGAGGTCT               GAGATTCGGACCCG               TGTCCTCCCCTGACGT GACTG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 002-101527279
Forward Primer: 5'-CATGCCCAGCCTAATGTCTTCTTC-3' T_m: 59.038
Reverse Primer: 5'-GCCAGAGGAGGTTATTTTCTTGCC-3' T_m: 59.038
Expected PCR Product Length: 427
Expected CelI Cleavage Product Sizes: 148, 279
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr2:101527402-101527447

Rank: 37	Homology Score: 10
	Genomic Region: Intron	Closest Gene: PITX3
Match Type: R20R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CGTCC TGAACCTGAGTCCTGG            GCCAATGTTCCCATCCCTGA            GCAGGAGGCAGGCAGA GATAT-3'
	3'-GCAGG ACTTGGACTCAGGACC            CGGTTACAAGGGTAGGGACT            CGTCCTCCGTCCGTCT CTATA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 010-103998000
Forward Primer: 5'-CTTCAGGTTAGTCTAGGAGGCATC-3' T_m: 59.038
Reverse Primer: 5'-TGACAGACGGGCCACCCTAT-3' T_m: 59.508
Expected PCR Product Length: 281
Expected CelI Cleavage Product Sizes: 144, 137
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr10:103998116-103998167

Rank: 38	Homology Score: 10
	Genomic Region: Intron	Closest Gene: GRIA4
Match Type: R21R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TATGT TGCACATGATTCCTTT           TAATCAACTCAACTGGAAGCC            AAAGCACACAGCTGCA GACCA-3'
	3'-ATACA ACGTGTACTAAGGAAA           ATTAGTTGAGTTGACCTTCGG            TTTCGTGTGTCGACGT CTGGT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 011-105719867
Forward Primer: 5'-CAGATTGCGCCACTGCATTCC-3' T_m: 59.378
Reverse Primer: 5'-TGTCTCCTTCAGAACTGAGCTGG-3' T_m: 59.148
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 136, 134
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr11:105719975-105720027

Rank: 39	Homology Score: 10
	Genomic Region: Intron	Closest Gene: COL25A1
Match Type: R11R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TGCAA TGCAGCGGGCTCCCCT                TACACACCTTT                 ACAGGAGTTAGTTGAA TATCA-3'
	3'-ACGTT ACGTCGCCCGAGGGGA                ATGTGTGGAAA                 TGTCCTCAATCAACTT ATAGT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 004-110007835
Forward Primer: 5'-GACTGCTGGGAATTGCTCTTTGAC-3' T_m: 59.038
Reverse Primer: 5'-TCCCTCAACTTCCACTGGGC-3' T_m: 59.508
Expected PCR Product Length: 290
Expected CelI Cleavage Product Sizes: 157, 133
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr4:110007969-110008011

Rank: 40	Homology Score: 10
	Genomic Region: Intron	Closest Gene: PHTF1
Match Type: R11R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TATTG TGAACTTGACTACAGA                CCTACTCGTGA                 ACAGGATTCAGCTCCA AAACG-3'
	3'-ATAAC ACTTGAACTGATGTCT                GGATGAGCACT                 TGTCCTAAGTCGAGGT TTTGC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 001-114253666
Forward Primer: 5'-AGGTGTTCTGGGAGGGGAGT-3' T_m: 59.508
Reverse Primer: 5'-GAGTAGATGGTGGTGGTGTGG-3' T_m: 59.378
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 131, 139
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr1:114253774-114253816

Rank: 41	Homology Score: 10
	Genomic Region: Intron	Closest Gene: KIRREL3
Match Type: R18R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TCTGT TGCACTTTTCTCTTCT             TAATCTATCGTTTGTTAC             ACGGGTGTCAGCTGCA AACCT-3'
	3'-AGACA ACGTGAAAAGAGAAGA             ATTAGATAGCAAACAATG             TGCCCACAGTCGACGT TTGGA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 011-126353104
Forward Primer: 5'-GAGGTCATACCCCCTTTTTTGTCC-3' T_m: 59.038
Reverse Primer: 5'-GGGCCCAGTTCTGAATCAAACAGC-3' T_m: 60.908
Expected PCR Product Length: 305
Expected CelI Cleavage Product Sizes: 175, 130
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr11:126353252-126353301

Rank: 42	Homology Score: 10
	Genomic Region: Intron	Closest Gene: POTEF
Match Type: R29R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-AGGAT TGCAGCCAGCTCCTGC       CCCGGTGCAGTGTATACAGTGTATACTGC        ACAGGTGTTGGGTGCA CCAAA-3'
	3'-TCCTA ACGTCGGTCGAGGACG       GGGCCACGTCACATATGTCACATATGACG        TGTCCACAACCCACGT GGTTT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Warning! Primer pair was generated using suboptimal parameters. 
Primer Index: 002-130879063
Forward Primer: 5'-AAGACGCCCACCGAGCCCA-3' T_m: 62.008
Reverse Primer: 5'-GCTAATGGGTGCAAAGTTTCTTTTAAGGAGCATA-3' T_m: 59.668
Expected PCR Product Length: 450
Expected CelI Cleavage Product Sizes: 144, 306
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr2:130879175-130879235

Rank: 43	Homology Score: 10
	Genomic Region: Intron	Closest Gene: LOC100507254
Match Type: R28R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-ATCAC TGGAACTGGTTCCAGT        GCGCATATATACTCTGATCAGCTTTTAC        ACAGGAGTCTGTGGCA GAGAA-3'
	3'-TAGTG ACCTTGACCAAGGTCA        CGCGTATATATGAGACTAGTCGAAAATG        TGTCCTCAGACACCGT CTCTT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 006-132465014
Forward Primer: 5'-GTTGTGTTGTGGCTGCTCAGAGT-3' T_m: 59.148
Reverse Primer: 5'-CCTTCTATCCCCATCCTCTCTTTC-3' T_m: 59.038
Expected PCR Product Length: 293
Expected CelI Cleavage Product Sizes: 136, 157
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr6:132465118-132465177

Rank: 44	Homology Score: 10
	Genomic Region: Intron	Closest Gene: MED27
Match Type: R27R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TTGTT TACAAATCACTCCTGC        CATCAACCTCATTTTACAGATCAGGAA         ACAGGAGTGAGCTGAA CAACG-3'
	3'-AACAA ATGTTTAGTGAGGACG        GTAGTTGGAGTAAAATGTCTAGTCCTT         TGTCCTCACTCGACTT GTTGC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 009-134936820
Forward Primer: 5'-GCTGGTAAGTGAAGTTGATGTGGC-3' T_m: 59.038
Reverse Primer: 5'-CCAGGAGCAGCTGCGATGAA-3' T_m: 59.508
Expected PCR Product Length: 318
Expected CelI Cleavage Product Sizes: 187, 131
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr9:134936976-134937034

Rank: 45	Homology Score: 10
	Genomic Region: Intron	Closest Gene: SPOCK1
Match Type: R12R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GGAAG TTTGCCAGGCTCCTGT                CCCTCCAGTGTG                ACAGGAGTGAGCTACA ATGCA-3'
	3'-CCTTC AAACGGTCCGAGGACA                GGGAGGTCACAC                TGTCCTCACTCGATGT TACGT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 005-136419378
Forward Primer: 5'-GCTATGGGAGAGCCGAAAACTG-3' T_m: 59.258
Reverse Primer: 5'-TGGAGTGGGCAAGCAAGAGC-3' T_m: 59.508
Expected PCR Product Length: 280
Expected CelI Cleavage Product Sizes: 150, 130
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr5:136419504-136419547

Rank: 46	Homology Score: 10
	Genomic Region: Intron	Closest Gene: FAM134B
Match Type: R20R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-AAGAT TGCCCCTGACTCAGGT            CCTTGGAAACCCAGAGTTGC            TCAGGATACAGTTGTA AGCAG-3'
	3'-TTCTA ACGGGGACTGAGTCCA            GGAACCTTTGGGTCTCAACG            AGTCCTATGTCAACAT TCGTC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 005-016519335
Forward Primer: 5'-CCTAGATCAGCGGCTTTCAAAGCA-3' T_m: 59.038
Reverse Primer: 5'-GGGCTGAAAGGAGCTTTGAGG-3' T_m: 59.378
Expected PCR Product Length: 374
Expected CelI Cleavage Product Sizes: 189, 185
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr5:16519496-16519547

Rank: 47	Homology Score: 10
	Genomic Region: Intron	Closest Gene: SH3RF1
Match Type: R14R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GTTTC TGTACCTCACTTCTGT               TTTCTGTGAATCAG               AGAGGATTTCGGTCCA TGAAT-3'
	3'-CAAAG ACATGGAGTGAAGACA               AAAGACACTTAGTC               TCTCCTAAAGCCAGGT ACTTA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 004-170092193
Forward Primer: 5'-GACCTAAAGGTTTCTCCACACATAGTGAAG-3' T_m: 60.058
Reverse Primer: 5'-GACTGTTCTGAGATCTTGGGTGTG-3' T_m: 59.038
Expected PCR Product Length: 350
Expected CelI Cleavage Product Sizes: 192, 158
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr4:170092360-170092405

Rank: 48	Homology Score: 10
	Genomic Region: Intron	Closest Gene: COL23A1
Match Type: R29R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-ATCCC TGCACCTCACACCTAT       CCTGAAGCAAACTAATCAGTAGTTAAATA        AGAGGAGGAGGGTACA TTACG-3'
	3'-TAGGG ACGTGGAGTGTGGATA       GGACTTCGTTTGATTAGTCATCAATTTAT        TCTCCTCCTCCCATGT AATGC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 005-177943719
Forward Primer: 5'-AAGCTTTCTGCCTCCTCAGGCC-3' T_m: 61.288
Reverse Primer: 5'-CACCTCAGTGACAGGCGCTT-3' T_m: 59.508
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 137, 133
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr5:177943824-177943884

Rank: 49	Homology Score: 10
	Genomic Region: Intron	Closest Gene: DENND4C
Match Type: R25R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GAGTC TGCAGATGACTTCTGT         TTATTTCCTATAGTACAAAATTAGT          ATGGGAGTGCTGTGCA CAGTG-3'
	3'-CTCAG ACGTCTACTGAAGACA         AATAAAGGATATCATGTTTTAATCA          TACCCTCACGACACGT GTCAC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 009-019336805
Forward Primer: 5'-GTCCATTGATAATCACTCTAGCACAGGTAC-3' T_m: 60.058
Reverse Primer: 5'-GTGTGACTTCCTTCCTTCCCTC-3' T_m: 59.258
Expected PCR Product Length: 422
Expected CelI Cleavage Product Sizes: 199, 223
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr9:19336974-19337030

Rank: 50	Homology Score: 10
	Genomic Region: Intron	Closest Gene: CCDC144C
Match Type: R13R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-AATGG TGCTCCTCATTCCCTT               CTGAGTTCAGAAC                ACAGGAGTCAGATAGA TGGAC-3'
	3'-TTACC ACGAGGAGTAAGGGAA               GACTCAAGTCTTG                TGTCCTCAGTCTATCT ACCTG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 017-020300643
Forward Primer: 5'-GGTGACAGAGCAAGACTCCATC-3' T_m: 59.258
Reverse Primer: 5'-CTCTGTGGGTTAGTGCCTAATCTG-3' T_m: 59.038
Expected PCR Product Length: 312
Expected CelI Cleavage Product Sizes: 181, 131
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr17:20300800-20300844

Rank: 51	Homology Score: 10
	Genomic Region: Intron	Closest Gene: WNT10A
Match Type: R26R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GTGTC TGCCCCAGACAGCTGG         GGCCAGAACCGAAACTGGGGCCCTGA         ACAGGAGCTGGGTGCA GACCC-3'
	3'-CACAG ACGGGGTCTGTCGACC         CCGGTCTTGGCTTTGACCCCGGGACT         TGTCCTCGACCCACGT CTGGG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 002-219751327
Forward Primer: 5'-TGTGTCTTAACGAGCCGCCC-3' T_m: 59.508
Reverse Primer: 5'-CTGACAGCTGTCATAGCCACG-3' T_m: 59.378
Expected PCR Product Length: 284
Expected CelI Cleavage Product Sizes: 154, 130
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr2:219751450-219751507

Rank: 52	Homology Score: 10
	Genomic Region: Intron	Closest Gene: CDC42BPA
Match Type: R26R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-ACTTC TGCAGCTGACTCAACA         ATCTGGGTTTCAACCACCCTTCCTCC         ATAGGATTCAGATGCA TGCCA-3'
	3'-TGAAG ACGTCGACTGAGTTGT         TAGACCCAAAGTTGGTGGGAAGGAGG         TATCCTAAGTCTACGT ACGGT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 001-227379954
Forward Primer: 5'-GAGATCACTCTCACCACCCAAAAC-3' T_m: 59.038
Reverse Primer: 5'-GGAAACAGCAGAACGTTGTTGGTG-3' T_m: 59.038
Expected PCR Product Length: 383
Expected CelI Cleavage Product Sizes: 190, 193
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr1:227380113-227380170

Rank: 53	Homology Score: 10
	Genomic Region: Intron	Closest Gene: C1orf198
Match Type: R20R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GGGCT TGCCCTTGACACCTGT            CTCAGGCCCAGTTTGGCTAC            ATGTGAGACTGGTGCA GAGTG-3'
	3'-CCCGA ACGGGAACTGTGGACA            GAGTCCGGGTCAAACCGATG            TACACTCTGACCACGT CTCAC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 001-230976433
Forward Primer: 5'-GCGCCAAGGTCTTCTTGAGC-3' T_m: 59.508
Reverse Primer: 5'-GGAGAGTAGGAGACAGCACATG-3' T_m: 59.258
Expected PCR Product Length: 312
Expected CelI Cleavage Product Sizes: 136, 176
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr1:230976541-230976592

Rank: 54	Homology Score: 10
	Genomic Region: Intron	Closest Gene: KLHL29
Match Type: R14R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TCCTG TGCACATGAGGCCTGT               GGCCATGTCAGGCC               CTGGGAGAGAGGTGCA AGTCT-3'
	3'-AGGAC ACGTGTACTCCGGACA               CCGGTACAGTCCGG               GACCCTCTCTCCACGT TCAGA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 002-023872858
Forward Primer: 5'-GTGGTCTTAGGCACCTCCTG-3' T_m: 59.508
Reverse Primer: 5'-CTCACTGTCCCCTTAACTTTCCTG-3' T_m: 59.038
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 136, 134
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr2:23872969-23873014

Rank: 55	Homology Score: 10
	Genomic Region: Intron	Closest Gene: APP
Match Type: R30R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CTCTT TGCAGCTGACCCCTTT       AACGTTACCCTCTAAAACATCTACTTCTTT       ACTGGAATTAGCTACA AGCAC-3'
	3'-GAGAA ACGTCGACTGGGGAAA       TTGCAATGGGAGATTTTGTAGATGAAGAAA       TGACCTTAATCGATGT TCGTG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 021-027390640
Forward Primer: 5'-CCTCCCTGCAGTATCTCATCTTTG-3' T_m: 59.038
Reverse Primer: 5'-GCAGGTCCATTCATTCCACCTC-3' T_m: 59.258
Expected PCR Product Length: 372
Expected CelI Cleavage Product Sizes: 214, 158
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr21:27390821-27390882

Rank: 56	Homology Score: 10
	Genomic Region: Intron	Closest Gene: BDNF-AS
Match Type: R12R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TCTGA TACCTCTGACTCCTGT                TTACCTAGAGGA                AGAAGAATCTGGAGCA GCAGT-3'
	3'-AGACT ATGGAGACTGAGGACA                AATGGATCTCCT                TCTTCTTAGACCTCGT CGTCA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 011-027668392
Forward Primer: 5'-GGTGGGTCCATTCTGACTCTG-3' T_m: 59.378
Reverse Primer: 5'-TCATGATCCACCCACCTCGG-3' T_m: 59.508
Expected PCR Product Length: 362
Expected CelI Cleavage Product Sizes: 219, 143
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr11:27668587-27668630

Rank: 57	Homology Score: 10
	Genomic Region: Intron	Closest Gene: TGFBR2
Match Type: R17R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-ATTCC TCCACCTGAGTCCTAT             TGAGGTGCTCCCCATTA              TAAGGAGTCCTGTGAA TCAAA-3'
	3'-TAAGG AGGTGGACTCAGGATA             ACTCCACGAGGGGTAAT              ATTCCTCAGGACACTT AGTTT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 003-030699112
Forward Primer: 5'-CTGGCACAGAGTGAGCACTTAATG-3' T_m: 59.038
Reverse Primer: 5'-GGTCGTCATGAGGAGAACACCTT-3' T_m: 59.148
Expected PCR Product Length: 327
Expected CelI Cleavage Product Sizes: 172, 155
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr3:30699258-30699306

Rank: 58	Homology Score: 10
	Genomic Region: Intron	Closest Gene: ZZEF1
Match Type: R29R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-ACTAC TGCCACAGACTCCTAA       CTGCTTTTCTTATTTCCACCTCTTTTCAC        ACAGCAGCCAGGGGCA CCTCT-3'
	3'-TGATG ACGGTGTCTGAGGATT       GACGAAAAGAATAAAGGTGGAGAAAAGTG        TGTCGTCGGTCCCCGT GGAGA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 017-003965044
Forward Primer: 5'-CGTAAGTCCTGACAACTCTACCTC-3' T_m: 59.038
Reverse Primer: 5'-GAAAGGCAGGACCCTTATCACTGT-3' T_m: 59.038
Expected PCR Product Length: 290
Expected CelI Cleavage Product Sizes: 143, 147
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr17:3965155-3965215

Rank: 59	Homology Score: 10
	Genomic Region: Intron	Closest Gene: SLC2A13
Match Type: R19R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TAAGA TTCAACTGACTCCTGA            GTTAGATGCAAAAAATTTT             ATAGTAGTGAGGTCTA CTGTT-3'
	3'-ATTCT AAGTTGACTGAGGACT            CAATCTACGTTTTTTAAAA             TATCATCACTCCAGAT GACAA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 012-040234817
Forward Primer: 5'-CCTTACAATTCACGGCTCTCAGAC-3' T_m: 59.038
Reverse Primer: 5'-CCACTGGAGTTGACAGATTTGGC-3' T_m: 59.148
Expected PCR Product Length: 436
Expected CelI Cleavage Product Sizes: 130, 306
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr12:40234920-40234970

Rank: 60	Homology Score: 10
	Genomic Region: Intron	Closest Gene: ANK1
Match Type: R15R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TATAA TGAACCTGGCTCCCGT              TCCTTTTATATAAGT               AGGGGAGTGAGGAGGA GGGGA-3'
	3'-ATATT ACTTGGACCGAGGGCA              AGGAAAATATATTCA               TCCCCTCACTCCTCCT CCCCT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 008-041570215
Forward Primer: 5'-GGGTGCAGAGAGATTTGCATGTG-3' T_m: 59.148
Reverse Primer: 5'-TGTCTCCTCCTGTCCCTGCT-3' T_m: 59.508
Expected PCR Product Length: 289
Expected CelI Cleavage Product Sizes: 153, 136
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr8:41570343-41570389

Rank: 61	Homology Score: 10
	Genomic Region: Intron	Closest Gene: TMEM72-AS1
Match Type: R29R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GGGGG TGCCCATGACTCCCCC       TTAGGTTTAACAATGCACTTGAATGACTC        ACAGGACTCAGGAGGA CACTC-3'
	3'-CCCCC ACGGGTACTGAGGGGG       AATCCAAATTGTTACGTGAACTTACTGAG        TGTCCTGAGTCCTCCT GTGAG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 010-045378787
Forward Primer: 5'-GGTGAGGCTCCATCCCACAA-3' T_m: 59.508
Reverse Primer: 5'-ACCCAGAGAGGGTGTGAGAG-3' T_m: 59.508
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 133, 137
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr10:45378888-45378948

Rank: 62	Homology Score: 10
	Genomic Region: Intron	Closest Gene: EIF2B3
Match Type: R12R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-AAAAA TGCAGCTCACACCTGT                GATCCCAGTACT                TTAGGAGGCAGAGGCA GGCAG-3'
	3'-TTTTT ACGTCGAGTGTGGACA                CTAGGGTCATGA                AATCCTCCGTCTCCGT CCGTC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 001-045422945
Forward Primer: 5'-GCCCCCAAACACCACAAAGTC-3' T_m: 59.378
Reverse Primer: 5'-ATTACAGGCACATGCCACCACG-3' T_m: 59.258
Expected PCR Product Length: 370
Expected CelI Cleavage Product Sizes: 240, 130
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr1:45423161-45423204

Rank: 63	Homology Score: 10
	Genomic Region: Intron	Closest Gene: SYMPK
Match Type: R23R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CCACC TGCTGCTGCTTCCTGA          GCCTGCTCTGCGGTGAATGCCAA           ACAGGAGCCAGGGCCA GGCTC-3'
	3'-GGTGG ACGACGACGAAGGACT          CGGACGAGACGCCACTTACGGTT           TGTCCTCGGTCCCGGT CCGAG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 019-046327426
Forward Primer: 5'-GTGCTTCGTTCACTGGTGAAGATG-3' T_m: 59.038
Reverse Primer: 5'-GTCCAGCTGTGGAAAAGTGATCTC-3' T_m: 59.038
Expected PCR Product Length: 284
Expected CelI Cleavage Product Sizes: 134, 150
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr19:46327531-46327585

Rank: 64	Homology Score: 10
	Genomic Region: Intron	Closest Gene: KRT82
Match Type: R23R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CCCCA TGCACCTCTCTCCTTT          CCCTAGAGCCTGGGCAAGGCCAG           GCTGGATTCAGCTTCA CCATT-3'
	3'-GGGGT ACGTGGAGAGAGGAAA          GGGATCTCGGACCCGTTCCGGTC           CGACCTAAGTCGAAGT GGTAA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 012-052789060
Forward Primer: 5'-ATTCCTCCCACCTCCTGCCA-3' T_m: 59.508
Reverse Primer: 5'-TTCAGGGCCCAGAAGCCACA-3' T_m: 59.508
Expected PCR Product Length: 267
Expected CelI Cleavage Product Sizes: 131, 136
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr12:52789162-52789216

Rank: 65	Homology Score: 10
	Genomic Region: Intron	Closest Gene: LNX1
Match Type: R10R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-ATACC TGCACCTGCCTCTTGC                 CCTTGTCATG                 ACAGGAACCAGGATAA ACAAC-3'
	3'-TATGG ACGTGGACGGAGAACG                 GGAACAGTAC                 TGTCCTTGGTCCTATT TGTTG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 004-054438610
Forward Primer: 5'-CCCAGGCCATTGCTTTTGATGC-3' T_m: 59.258
Reverse Primer: 5'-GGCTGCCTCTCATTTTGTCCAC-3' T_m: 59.258
Expected PCR Product Length: 279
Expected CelI Cleavage Product Sizes: 134, 145
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr4:54438721-54438762

Rank: 66	Homology Score: 10
	Genomic Region: Intron	Closest Gene: CDH4
Match Type: R13R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TGCAT TTCTCCTGACTTGTAT               TACTCATTTTACG                GCTGGAGACAGGTGCA CATGG-3'
	3'-ACGTA AAGAGGACTGAACATA               ATGAGTAAAATGC                CGACCTCTGTCCACGT GTACC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 020-059992627
Forward Primer: 5'-TGGCCCTGTTTCTGGCTGGA-3' T_m: 59.508
Reverse Primer: 5'-AGTACACTGAAGTGCAGTCTCTGG-3' T_m: 59.038
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 136, 134
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr20:59992739-59992783

Rank: 67	Homology Score: 10
	Genomic Region: Intron	Closest Gene: CDH4
Match Type: R23R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TCTGA TGCCCCTGACTCCTCC          CGGCTCCCTCCCCCAGGACTACG           ACCTCAGCCAGCTGCA GCAGC-3'
	3'-AGACT ACGGGGACTGAGGAGG          GCCGAGGGAGGGGGTCCTGATGC           TGGAGTCGGTCGACGT CGTCG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 020-060508972
Forward Primer: 5'-CATGGTTCTGTGGGATGCTTGG-3' T_m: 59.258
Reverse Primer: 5'-ACCATGGGCCTGATCGGGTA-3' T_m: 59.508
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 139, 131
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr20:60509082-60509136

Rank: 68	Homology Score: 10
	Genomic Region: Intron	Closest Gene: PCDH9
Match Type: R17R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-ATGCT TGCACCACAGTTCTTT             GTGAATGGGGGATGTGG              ACTGGATTCAGGTGAA AGAAA-3'
	3'-TACGA ACGTGGTGTCAAGAAA             CACTTACCCCCTACACC              TGACCTAAGTCCACTT TCTTT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 013-067512369
Forward Primer: 5'-GGCATCAACAGGGCTTCTTAAACG-3' T_m: 59.038
Reverse Primer: 5'-CAATGTCAGTCGTTTCTGCATGAAGATAGG-3' T_m: 60.058
Expected PCR Product Length: 388
Expected CelI Cleavage Product Sizes: 227, 161
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr13:67512570-67512618

Rank: 69	Homology Score: 10
	Genomic Region: Intron	Closest Gene: PRMT7
Match Type: R22R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GGAAA TGCACGTGGCTCCTGC           TTGTCATCCCAGCACTCTGGGA           ACCTGAGGCTGGTGGA TTGCT-3'
	3'-CCTTT ACGTGCACCGAGGACG           AACAGTAGGGTCGTGAGACCCT           TGGACTCCGACCACCT AACGA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 016-068387900
Forward Primer: 5'-GCGCCAGTGGTCCTTGGATT-3' T_m: 59.508
Reverse Primer: 5'-CTGGGAATACAGGTGTGCACTAC-3' T_m: 59.148
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 134, 136
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr16:68388005-68388058

Rank: 70	Homology Score: 10
	Genomic Region: Intron	Closest Gene: GOLGA6B
Match Type: R15R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CTGCC TGCACCTGGCTCATCT              GTTGGCCTTGGCCTG               GAAGGAGCCAGAGGCA GAGGC-3'
	3'-GACGG ACGTGGACCGAGTAGA              CAACCGGAACCGGAC               CTTCCTCGGTCTCCGT CTCCG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 015-072957610
Forward Primer: 5'-TACTCACCTCTTGCCTTCAAGTGG-3' T_m: 59.038
Reverse Primer: 5'-CTTTTCTTGGCCCATGCCAGG-3' T_m: 59.378
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 133, 137
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr15:72957718-72957764

Rank: 71	Homology Score: 10
	Genomic Region: Intron	Closest Gene: PIBF1
Match Type: R23R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-AAATT TACCCCTCTCTCCTCT          GAATTTAGAACTATTAAGGAGAA           ACAAGAGTCAGTTACA TATCT-3'
	3'-TTTAA ATGGGGAGAGAGGAGA          CTTAAATCTTGATAATTCCTCTT           TGTTCTCAGTCAATGT ATAGA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 013-073489472
Forward Primer: 5'-GCGCTCTGCTTCTAAGACACATTG-3' T_m: 59.038
Reverse Primer: 5'-CACATGGAAGAGATGATACATGCCATTACC-3' T_m: 60.058
Expected PCR Product Length: 386
Expected CelI Cleavage Product Sizes: 243, 143
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr13:73489686-73489740

Rank: 72	Homology Score: 10
	Genomic Region: Intron	Closest Gene: SLC16A2
Match Type: R21R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GCATG TTTACCTGATCCCTGA           TGTTCCAATCTCATAGTGACC            ACAGGAGCCTGGAGCA TAGTA-3'
	3'-CGTAC AAATGGACTAGGGACT           ACAAGGTTAGAGTATCACTGG            TGTCCTCGGACCTCGT ATCAT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 00X-073723246
Forward Primer: 5'-GTACAGTGGGGAGTCTGGAG-3' T_m: 59.508
Reverse Primer: 5'-CAGAAGACCAGAAGTAGGCCTAAC-3' T_m: 59.038
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 135, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chrX:73723353-73723405

Rank: 73	Homology Score: 10
	Genomic Region: Intron	Closest Gene: GOLGA6A
Match Type: R15R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GCCTC TGCCTCTGGCTCCTTC              CAGGCGAAGGCCAAC               AGATGAGCCAGGTGCA GGCAG-3'
	3'-CGGAG ACGGAGACCGAGGAAG              GTCCGCTTCCGGTTG               TCTACTCGGTCCACGT CCGTC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 015-074364061
Forward Primer: 5'-TTGGCCCATGCCAGGACTCA-3' T_m: 59.508
Reverse Primer: 5'-GCCCCTACAAGAATACTCACCTC-3' T_m: 59.148
Expected PCR Product Length: 277
Expected CelI Cleavage Product Sizes: 134, 143
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr15:74364170-74364216

Rank: 74	Homology Score: 10
	Genomic Region: Intron	Closest Gene: MBP
Match Type: R20R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GGCTC TGCTCCTCTCTCCTGT            CTTGGCTGGCAGGGGCTGCC            CCTGGGGTCGGGTGGA CTCCA-3'
	3'-CCGAG ACGAGGAGAGAGGACA            GAACCGACCGTCCCCGACGG            GGACCCCAGCCCACCT GAGGT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 018-074790610
Forward Primer: 5'-GAGCTGTGGACTGAAGAGCAAC-3' T_m: 59.258
Reverse Primer: 5'-CACAGTCTTGGGAGGGTGCT-3' T_m: 59.508
Expected PCR Product Length: 304
Expected CelI Cleavage Product Sizes: 133, 171
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr18:74790715-74790766

Rank: 75	Homology Score: 10
	Genomic Region: Intron	Closest Gene: GOLGA6C
Match Type: R15R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CCGCC TGCACCTGGCTCATCT              GGTGGCCTTGGCCTG               GAAGGAGCCAGAGGCA GAGGC-3'
	3'-GGCGG ACGTGGACCGAGTAGA              CCACCGGAACCGGAC               CTTCCTCGGTCTCCGT CTCCG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 015-075561464
Forward Primer: 5'-TACTCACCTCTTGCCTTCAAGTGG-3' T_m: 59.038
Reverse Primer: 5'-TTCTTGGCCCATGCCAGGAC-3' T_m: 59.508
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 133, 137
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr15:75561572-75561618

Rank: 76	Homology Score: 10
	Genomic Region: Intron	Closest Gene: GOLGA6D
Match Type: R15R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CTGCC TGCACCTGGCTCATCT              GGTGGCCTTGGCCTG               GAAGGAGCCAGAGGCA GAGGC-3'
	3'-GACGG ACGTGGACCGAGTAGA              CCACCGGAACCGGAC               CTTCCTCGGTCTCCGT CTCCG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 015-075585740
Forward Primer: 5'-TACTCACCTCTTGCCTTCAAGTGG-3' T_m: 59.038
Reverse Primer: 5'-TTCTTGGCCCATGCCAGGAC-3' T_m: 59.508
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 133, 137
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr15:75585848-75585894

Rank: 77	Homology Score: 10
	Genomic Region: Intron	Closest Gene: IQGAP2
Match Type: R29R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TGGTC TGAAAGAGACTCGTGT       GAAGGTCTAGAACAGGAAGGGGGAAGTAA        ACGGGTGTGAGGTGCA GCCAG-3'
	3'-ACCAG ACTTTCTCTGAGCACA       CTTCCAGATCTTGTCCTTCCCCCTTCATT        TGCCCACACTCCACGT CGGTC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 005-075922611
Forward Primer: 5'-AGGGTAGCCCGGGAAGACTTT-3' T_m: 59.378
Reverse Primer: 5'-TAAAGCTTCCCCAAAGGCTTCCC-3' T_m: 59.148
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 135, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr5:75922714-75922774

Rank: 78	Homology Score: 10
	Genomic Region: Intron	Closest Gene: C14orf166B
Match Type: R24R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TAACT TGCCCCCAACTCATCT          TTTCTTAGATTAGCTCTGGCAATC          ACAGGAGGCAGATGTA ACAGT-3'
	3'-ATTGA ACGGGGGTTGAGTAGA          AAAGAATCTAATCGAGACCGTTAG          TGTCCTCCGTCTACAT TGTCA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 014-077327823
Forward Primer: 5'-CCAACAGCTGGTAAGGATGTCAAG-3' T_m: 59.038
Reverse Primer: 5'-CTGTGGAAACACCAAAGCAGGG-3' T_m: 59.258
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 133, 137
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr14:77327926-77327981

Rank: 79	Homology Score: 10
	Genomic Region: Intron	Closest Gene: TMED3
Match Type: R17R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TCCTG TATTCCTGTCTCCTTT             GAGAGATCCCGTGGCCC              ACAGGTGACAGGAGCA GGTCT-3'
	3'-AGGAC ATAAGGACAGAGGAAA             CTCTCTAGGGCACCGGG              TGTCCACTGTCCTCGT CCAGA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 015-079604259
Forward Primer: 5'-TTGGAAAGGAGGGATCAGTGCAG-3' T_m: 59.148
Reverse Primer: 5'-GTGCCTATCAGCATGCGTCC-3' T_m: 59.508
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 135, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr15:79604368-79604416

Rank: 80	Homology Score: 10
	Genomic Region: Intron	Closest Gene: ICA1
Match Type: R26R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GGGTT TTCTCCTGGCTCCACT         CCAGTTTCCTCCCATATCCCAAAGCT         ACACAAGTCAGGTGAA TGTGC-3'
	3'-CCCAA AAGAGGACCGAGGTGA         GGTCAAAGGAGGGTATAGGGTTTCGA         TGTGTTCAGTCCACTT ACACG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 007-008187341
Forward Primer: 5'-CACACACACACACACACACAAGC-3' T_m: 59.148
Reverse Primer: 5'-ACACAGGATGGGAACCAGTCCTA-3' T_m: 59.148
Expected PCR Product Length: 370
Expected CelI Cleavage Product Sizes: 240, 130
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr7:8187550-8187607

Rank: 81	Homology Score: 10
	Genomic Region: Intron	Closest Gene: LINC00299
Match Type: R25R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GCTTT TGCACCTGCCCCCAGT         GCTGTAAGTTCTATGGAAATTCCAC          CCTGGTGTCAGCTCCA CACTG-3'
	3'-CGAAA ACGTGGACGGGGGTCA         CGACATTCAAGATACCTTTAAGGTG          GGACCACAGTCGAGGT GTGAC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 002-008374518
Forward Primer: 5'-TCCCGGAAACATGTCAGCCC-3' T_m: 59.508
Reverse Primer: 5'-CATCCTACATCCCCTGAACATGG-3' T_m: 59.148
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 139, 131
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr2:8374627-8374683

Rank: 82	Homology Score: 10
	Genomic Region: Intron	Closest Gene: ME1
Match Type: R22R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CTGTT TGCACTTGACATATCT           GAATAAGATTTCTTTAAATAAG           ACAGGAGTGTCGTGCA CTGAT-3'
	3'-GACAA ACGTGAACTGTATAGA           CTTATTCTAAAGAAATTTATTC           TGTCCTCACAGCACGT GACTA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 006-083995632
Forward Primer: 5'-CAGTTGTTCGGATACGGCTTATCTTCATG-3' T_m: 60.178
Reverse Primer: 5'-GGGTAAGAAAACAGAGGCACAGAG-3' T_m: 59.038
Expected PCR Product Length: 307
Expected CelI Cleavage Product Sizes: 171, 136
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr6:83995774-83995827

Rank: 83	Homology Score: 10
	Genomic Region: Intron	Closest Gene: NXPH1
Match Type: R21R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-ATTAA TGCAACAGACTTCTGT           ACTTAGAAGAACTGCCTAAGA            AGAGAACTCAGGTTAA CTCTG-3'
	3'-TAATT ACGTTGTCTGAAGACA           TGAATCTTCTTGACGGATTCT            TCTCTTGAGTCCAATT GAGAC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 007-008646994
Forward Primer: 5'-GGATCGTGTCACTCGATACAGTAC-3' T_m: 59.038
Reverse Primer: 5'-GGGTCTAGCAACATATTCCCACAG-3' T_m: 59.038
Expected PCR Product Length: 329
Expected CelI Cleavage Product Sizes: 179, 150
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr7:8647145-8647197

Rank: 84	Homology Score: 10
	Genomic Region: Intron	Closest Gene: NTN1
Match Type: R16R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GGAAT TACAGAGGACTCCTGG              GGAATTAGCCACATCC              ACAGCAGCCAGGAGCA CCAGG-3'
	3'-CCTTA ATGTCTCCTGAGGACC              CCTTAATCGGTGTAGG              TGTCGTCGGTCCTCGT GGTCC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 017-009048384
Forward Primer: 5'-AGCTGAGGCAGGCGCATGAA-3' T_m: 59.508
Reverse Primer: 5'-CCCGGTGGCGTTGTTTTATGAC-3' T_m: 59.258
Expected PCR Product Length: 313
Expected CelI Cleavage Product Sizes: 131, 182
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr17:9048489-9048536

Rank: 85	Homology Score: 10
	Genomic Region: Intron	Closest Gene: USP43
Match Type: R21R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TCATG TGAAACTGACTCCTGA           GAAAATGTGGGCAGGAATGAT            TTAGCAGGCAGGTGGA AGGTG-3'
	3'-AGTAC ACTTTGACTGAGGACT           CTTTTACACCCGTCCTTACTA            AATCGTCCGTCCACCT TCCAC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 017-009569404
Forward Primer: 5'-GGGAATGAAAAGGACAGCAGGAC-3' T_m: 59.148
Reverse Primer: 5'-CGGCCCTGCGCGTTATTTCT-3' T_m: 59.508
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 135, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr17:9569507-9569559

Rank: 86	Homology Score: 10
	Genomic Region: Intron	Closest Gene: USP43
Match Type: R28R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TCATG TGAAACTGACTCCTGA        GAAAATGTGGGCAGGAATGATTTAGCAG        GCAGGTGGAAGGTGGA GAGAT-3'
	3'-AGTAC ACTTTGACTGAGGACT        CTTTTACACCCGTCCTTACTAAATCGTC        CGTCCACCTTCCACCT CTCTA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 017-009569404
Forward Primer: 5'-GGGAATGAAAAGGACAGCAGGAC-3' T_m: 59.148
Reverse Primer: 5'-CGGCCCTGCGCGTTATTTCT-3' T_m: 59.508
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 135, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr17:9569507-9569566

Rank: 87	Homology Score: 10
	Genomic Region: Intron	Closest Gene: LOC100616530
Match Type: R24R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GAGCT TGGACCTGTCTCCTCT          GCATCTGCAGTGGAGCCACGCCTC          ACTCGACTCAGTAGCA GGTCA-3'
	3'-CTCGA ACCTGGACAGAGGAGA          CGTAGACGTCACCTCGGTGCGGAG          TGAGCTGAGTCATCGT CCAGT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 008-096438369
Forward Primer: 5'-GACCAAGCCCCAGAATGGTTAATC-3' T_m: 59.038
Reverse Primer: 5'-CAGCTCCCCCTAAACTACCAG-3' T_m: 59.378
Expected PCR Product Length: 310
Expected CelI Cleavage Product Sizes: 178, 132
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr8:96438517-96438572

Rank: 88	Homology Score: 10
	Genomic Region: Intergenic	Closest Gene: LOC100129138
Match Type: R16R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CATGA TTTACCTGAATGCAGT              TCTTTCTCTCTTACCA              ACAGAAGTGAGGTTCA GGGCC-3'
	3'-GTACT AAATGGACTTACGTCA              AGAAAGAGAGAATGGT              TGTCTTCACTCCAAGT CCCGG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 001-104689409
Forward Primer: 5'-GTTGAGGGAGTCATCCTGAGC-3' T_m: 59.378
Reverse Primer: 5'-GGTTTCACTGTGTTAGCCAGGATG-3' T_m: 59.038
Expected PCR Product Length: 334
Expected CelI Cleavage Product Sizes: 204, 130
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr1:104689587-104689634

Rank: 89	Homology Score: 10
	Genomic Region: Intergenic	Closest Gene: AIM1
Match Type: R24R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CTCAG TGTCCCAGACTGCAGT          CTATGACTGTGGTGGTATGGTTTT          GCAGGGGTCAGGGGCA CTAAG-3'
	3'-GAGTC ACAGGGTCTGACGTCA          GATACTGACACCACCATACCAAAA          CGTCCCCAGTCCCCGT GATTC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 006-106935880
Forward Primer: 5'-TGGTGCATTGACTTTGGTTCTGGG-3' T_m: 59.038
Reverse Primer: 5'-TCTGACCCAGACCTGACAGATAG-3' T_m: 59.148
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 132, 138
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr6:106935982-106936037

Rank: 90	Homology Score: 10
	Genomic Region: Intergenic	Closest Gene: ABRA
Match Type: R28R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TTCTT TGGACCTGACTCCTTG        AAAGTGAGCTGTCCTTCTTATGTATCCA        AAAAGTCTCAGGTGTA TTATA-3'
	3'-AAGAA ACCTGGACTGAGGAAC        TTTCACTCGACAGGAAGAATACATAGGT        TTTTCAGAGTCCACAT AATAT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 008-107911219
Forward Primer: 5'-GGCTTCACTTTAGGTCTCTCTTCC-3' T_m: 59.038
Reverse Primer: 5'-CCATAGCAAGCCATTTCAGCCTTC-3' T_m: 59.038
Expected PCR Product Length: 401
Expected CelI Cleavage Product Sizes: 248, 153
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr8:107911435-107911494

Rank: 91	Homology Score: 10
	Genomic Region: Intergenic	Closest Gene: DOCK6
Match Type: R15R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CCAGC TGCCCCTGCCTCCTGG              ATGCTTCTCTAAACA               CCAGGACACAGCTCCA CTCAA-3'
	3'-GGTCG ACGGGGACGGAGGACC              TACGAAGAGATTTGT               GGTCCTGTGTCGAGGT GAGTT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 019-011375833
Forward Primer: 5'-GAGAGTGAGACCCTGTCTCAAAAC-3' T_m: 59.038
Reverse Primer: 5'-TAGGCTACATTAGGGCGAGTCC-3' T_m: 59.258
Expected PCR Product Length: 321
Expected CelI Cleavage Product Sizes: 180, 141
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr19:11375988-11376034

Rank: 92	Homology Score: 10
	Genomic Region: Intergenic	Closest Gene: MAN1A2
Match Type: R11R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-ATCTT TGACCCTGCCTCCTGT                CTTTATTTCAA                 AAAGGATGCAATTGCA AAGAT-3'
	3'-TAGAA ACTGGGACGGAGGACA                GAAATAAAGTT                 TTTCCTACGTTAACGT TTCTA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Warning! Primer pair was generated using suboptimal parameters. 
Primer Index: 001-118086389
Forward Primer: 5'-CTGTTCATTTTCTTAAGGTGGAGACTTACCTG-3' T_m: 59.848
Reverse Primer: 5'-GCACTCTGGAAACATTCTCTTTCAATGTGTC-3' T_m: 59.958
Expected PCR Product Length: 448
Expected CelI Cleavage Product Sizes: 270, 178
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr1:118086636-118086678

Rank: 93	Homology Score: 10
	Genomic Region: Intergenic	Closest Gene: NDST3
Match Type: R26R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TTACA TGCATCTATCTCATTT         AATGCTTTTAACAATGCTACAACTAA         AAAGTAGTCAGGTGTA GACTG-3'
	3'-AATGT ACGTAGATAGAGTAAA         TTACGAAAATTGTTACGATGTTGATT         TTTCATCAGTCCACAT CTGAC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Warning! Primer pair was generated using suboptimal parameters. 
Primer Index: 004-118702135
Forward Primer: 5'-GCAGGGACACTGGTGTTATGTCT-3' T_m: 59.148
Reverse Primer: 5'-GATAGTAACTTATTTAGAATTGAGGTTGTGTGATGGAG-3' T_m: 59.358
Expected PCR Product Length: 429
Expected CelI Cleavage Product Sizes: 293, 136
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr4:118702397-118702454

Rank: 94	Homology Score: 10
	Genomic Region: Intergenic	Closest Gene: HAS2
Match Type: R18R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CCTCT TGGACCTGACACCTCT             TCCCCAAACCAGAGGAAC             TCTGTAGCCAGGGGCA TCAAC-3'
	3'-GGAGA ACCTGGACTGTGGAGA             AGGGGTTTGGTCTCCTTG             AGACATCGGTCCCCGT AGTTG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 008-122428182
Forward Primer: 5'-ACAAGTTCTGCCAACTACACCAGG-3' T_m: 59.038
Reverse Primer: 5'-AGAGCCATTCCCAAGTGGGGT-3' T_m: 59.378
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 134, 136
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr8:122428289-122428338

Rank: 95	Homology Score: 10
	Genomic Region: Intergenic	Closest Gene: POTEE
Match Type: R29R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TTTGG TGCACCCAACACCTGT       GCAGTATACACTGTATACACTGCACCGGG        GCAGGAGCTGGCTGCA ATCCT-3'
	3'-AAACC ACGTGGGTTGTGGACA       CGTCATATGTGACATATGTGACGTGGCCC        CGTCCTCGACCGACGT TAGGA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Warning! Primer pair was generated using suboptimal parameters. 
Primer Index: 002-131974522
Forward Primer: 5'-GCTAATGGGTGCAAAGTTTCTTTTAAGGAGCATA-3' T_m: 59.668
Reverse Primer: 5'-AAGACGCCCACCGAGCCCA-3' T_m: 62.008
Expected PCR Product Length: 450
Expected CelI Cleavage Product Sizes: 309, 141
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr2:131974799-131974859

Rank: 96	Homology Score: 10
	Genomic Region: Intergenic	Closest Gene: PCDH10
Match Type: R26R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CCAAT TGCATCAGATACCTGA         TTTAGCACACTGATTAAAAGGAATGA         ACTGTAGTCAGGTTCA GTGGC-3'
	3'-GGTTA ACGTAGTCTATGGACT         AAATCGTGTGACTAATTTTCCTTACT         TGACATCAGTCCAAGT CACCG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 004-132113714
Forward Primer: 5'-GCTGTTCTGAGGTTGTTCCTGTG-3' T_m: 59.148
Reverse Primer: 5'-CCAGACTGAAGCATAGTCCCATGA-3' T_m: 59.038
Expected PCR Product Length: 291
Expected CelI Cleavage Product Sizes: 151, 140
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr4:132113834-132113891

Rank: 97	Homology Score: 10
	Genomic Region: Intergenic	Closest Gene: ARAP3
Match Type: R23R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TGTTA TGCTCTTGGCTCCTAG          CCAGTGGTTATATGACATCAGGG           AAAGGACACAGGTGCA TGGAG-3'
	3'-ACAAT ACGAGAACCGAGGATC          GGTCACCAATATACTGTAGTCCC           TTTCCTGTGTCCACGT ACCTC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 005-141074133
Forward Primer: 5'-TCTGCTTGGGCCTTAGAGGG-3' T_m: 59.508
Reverse Primer: 5'-ATTCAGCATCCCTGCCCCAGA-3' T_m: 59.378
Expected PCR Product Length: 292
Expected CelI Cleavage Product Sizes: 139, 153
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr5:141074243-141074297

Rank: 98	Homology Score: 10
	Genomic Region: Intergenic	Closest Gene: FLJ43860
Match Type: R24R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TTTGT TAAACCACATTCCTGT          GAGAGGCAACTTTTCAACTGGAGA          ACAGTACTCATGTGCA GTTCC-3'
	3'-AAACA ATTTGGTGTAAGGACA          CTCTCCGTTGAAAAGTTGACCTCT          TGTCATGAGTACACGT CAAGG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 008-142885698
Forward Primer: 5'-GTGAACTACCTCTCTGGCACTG-3' T_m: 59.258
Reverse Primer: 5'-CTCACCCAAAACTGGAAGCAACC-3' T_m: 59.148
Expected PCR Product Length: 336
Expected CelI Cleavage Product Sizes: 132, 204
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr8:142885800-142885855

Rank: 99	Homology Score: 10
	Genomic Region: Intergenic	Closest Gene: AFF2
Match Type: R21R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GGATT TGAGCCTGACTTCTCA           GCCCTAATGCTCCTTTACAGG            ACAGGAGCCATGTGAA ATTAA-3'
	3'-CCTAA ACTCGGACTGAAGAGT           CGGGATTACGAGGAAATGTCC            TGTCCTCGGTACACTT TAATT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 00X-148285072
Forward Primer: 5'-GGAGCAAAAAGGACTATGTCACTGAGTCAA-3' T_m: 60.058
Reverse Primer: 5'-CCACGTAAGTCCTACAGGTGACTA-3' T_m: 59.038
Expected PCR Product Length: 450
Expected CelI Cleavage Product Sizes: 307, 143
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chrX:148285351-148285403

Rank: 100	Homology Score: 10
	Genomic Region: Intergenic	Closest Gene: DCLK2
Match Type: R24R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GCAGG TTGACTTGACTCCTTG          TGGAATCACCACAGCATGACCTGG          ACAGGTGTCAGTGGCA ACAGC-3'
	3'-CGTCC AACTGAACTGAGGAAC          ACCTTAGTGGTGTCGTACTGGACC          TGTCCACAGTCACCGT TGTCG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 004-150284205
Forward Primer: 5'-GAGGAAGTCCCCAGTGAGAAAATG-3' T_m: 59.038
Reverse Primer: 5'-TATTCCTGGACCCACCCTACTTC-3' T_m: 59.148
Expected PCR Product Length: 309
Expected CelI Cleavage Product Sizes: 179, 130
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr4:150284354-150284409

Rank: 101	Homology Score: 10
	Genomic Region: Intergenic	Closest Gene: LOC285889
Match Type: R19R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-ATTGA TGGACTTGACACCTGT            ATCCCTGGAGGGTCTGTCC             TCAGCAGTGGGCTGCA GCCCA-3'
	3'-TAACT ACCTGAACTGTGGACA            TAGGGACCTCCCAGACAGG             AGTCGTCACCCGACGT CGGGT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 007-156274056
Forward Primer: 5'-CAGGCGAGGTCTTACCCGAT-3' T_m: 59.508
Reverse Primer: 5'-GTCCACGTGTTCCCATTTTTGGC-3' T_m: 59.148
Expected PCR Product Length: 383
Expected CelI Cleavage Product Sizes: 130, 253
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr7:156274159-156274209

Rank: 102	Homology Score: 10
	Genomic Region: Intergenic	Closest Gene: LRP2
Match Type: R13R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GAACG TGCACCAGAATTCAGC               CAAGCTCCCTATC                AAAGGAGTCAGGTCTA TCTTT-3'
	3'-CTTGC ACGTGGTCTTAAGTCG               GTTCGAGGGATAG                TTTCCTCAGTCCAGAT AGAAA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 002-170227380
Forward Primer: 5'-GGCCAGAAGACCAAGATGATTCC-3' T_m: 59.148
Reverse Primer: 5'-GCCAAAAGCCAAATGTAGACTTTGTGCTG-3' T_m: 60.178
Expected PCR Product Length: 403
Expected CelI Cleavage Product Sizes: 259, 144
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr2:170227615-170227659

Rank: 103	Homology Score: 10
	Genomic Region: Intergenic	Closest Gene: MSX2
Match Type: R16R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TTTGT TGATCCTGTGTCCAGT              GCTGTGGCTGGAAATG              ACAGCAGTCACGTTCA TGCAC-3'
	3'-AAACA ACTAGGACACAGGTCA              CGACACCGACCTTTAC              TGTCGTCAGTGCAAGT ACGTG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 005-174033940
Forward Primer: 5'-CAGGAAGTCTTCCTTGACTGCAC-3' T_m: 59.148
Reverse Primer: 5'-GGCAGGCATTTTCCAGATGAGG-3' T_m: 59.258
Expected PCR Product Length: 330
Expected CelI Cleavage Product Sizes: 186, 144
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr5:174034100-174034147

Rank: 104	Homology Score: 10
	Genomic Region: Intergenic	Closest Gene: LINC00290
Match Type: R30R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GCTTC TGCTCCGAAGTCCTGC       ATGGACATGCTTGTATATATTTATACCATT       AAAGGAGTCAGTTTCA AAAAT-3'
	3'-CGAAG ACGAGGCTTCAGGACG       TACCTGTACGAACATATATAAATATGGTAA       TTTCCTCAGTCAAAGT TTTTA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 004-182299969
Forward Primer: 5'-GACAAGGAAACTGAGGCACAGAAG-3' T_m: 59.038
Reverse Primer: 5'-ACACTGATTCCTGACCTCAGCG-3' T_m: 59.258
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 138, 132
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr4:182300074-182300135

Rank: 105	Homology Score: 10
	Genomic Region: Intergenic	Closest Gene: SH3YL1
Match Type: R19R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-AAAAG TGAACATGAGTCATAT            GTTACCTGACTTGTTCAAT             ACATGAGTCAGGACCA CCTTC-3'
	3'-TTTTC ACTTGTACTCAGTATA            CAATGGACTGAACAAGTTA             TGTACTCAGTCCTGGT GGAAG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 002-000185249
Forward Primer: 5'-CCACTGACCATAGACTGGTTCTG-3' T_m: 59.148
Reverse Primer: 5'-CCTGAACATTCCTGCAACCTTCAC-3' T_m: 59.038
Expected PCR Product Length: 392
Expected CelI Cleavage Product Sizes: 157, 235
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr2:185379-185429

Rank: 106	Homology Score: 10
	Genomic Region: Intergenic	Closest Gene: KCNT2
Match Type: R10R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CAGAT TGGACGTGACTACTGT                 GCCCTTGCCC                 AGAGGAGTCCTATGTA TCCAT-3'
	3'-GTCTA ACCTGCACTGATGACA                 CGGGAACGGG                 TCTCCTCAGGATACAT AGGTA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 001-195936317
Forward Primer: 5'-GGACCATCAGCAGATAGTACGAAG-3' T_m: 59.038
Reverse Primer: 5'-GTTGGGGTCAGTTTCCTAGGC-3' T_m: 59.378
Expected PCR Product Length: 383
Expected CelI Cleavage Product Sizes: 251, 132
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr1:195936545-195936586

Rank: 107	Homology Score: 10
	Genomic Region: Intergenic	Closest Gene: MIR4686
Match Type: R25R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TCGGC TCCACCTGACTCCTAC         ACTGGCTCTGCAACCATTCCCAGCC          ACAGAGGTTGGCTGCA AGTAC-3'
	3'-AGCCG AGGTGGACTGAGGATG         TGACCGAGACGTTGGTAAGGGTCGG          TGTCTCCAACCGACGT TCATG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 011-002239013
Forward Primer: 5'-AGCTGTCCAGCCTGCAGCAT-3' T_m: 59.508
Reverse Primer: 5'-TCATATTCCTGGGTGGTCACTCC-3' T_m: 59.148
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 138, 132
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr11:2239121-2239177

Rank: 108	Homology Score: 10
	Genomic Region: Intergenic	Closest Gene: COG2
Match Type: R28R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TTATT TGAACATGACTTCTGT        GCATTCCATTGCACCATGTGGTGGGGAG        AAAGGTGTCTGGTCTA TTTTT-3'
	3'-AATAA ACTTGTACTGAAGACA        CGTAAGGTAACGTGGTACACCACCCCTC        TTTCCACAGACCAGAT AAAAA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 001-230749531
Forward Primer: 5'-CAAAGATGCAAAGGCTGATTTAGAGCACC-3' T_m: 60.178
Reverse Primer: 5'-CCCCAAATTTAGCAATACTGTCTGCTGGA-3' T_m: 60.178
Expected PCR Product Length: 441
Expected CelI Cleavage Product Sizes: 131, 310
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr1:230749630-230749689

Rank: 109	Homology Score: 10
	Genomic Region: Intergenic	Closest Gene: PPARGC1A
Match Type: R13R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-ACAAA TGCACCCTACTCATGT               AAGATGTTATAAC                AGGGGAGACTGGGGCA GGGCA-3'
	3'-TGTTT ACGTGGGATGAGTACA               TTCTACAATATTG                TCCCCTCTGACCCCGT CCCGT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 004-024170953
Forward Primer: 5'-CTGGATGGGATCCCAGAACAGA-3' T_m: 59.258
Reverse Primer: 5'-GAGCCAACATGCTTGGCTGAG-3' T_m: 59.378
Expected PCR Product Length: 276
Expected CelI Cleavage Product Sizes: 140, 136
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr4:24171069-24171113

Rank: 110	Homology Score: 10
	Genomic Region: Intergenic	Closest Gene: LOC200772
Match Type: R29R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CCCAC TGCACCTGTCACCTCT       GGCCCAGCACAAGGGCTGCATGTGGCCAC        ACCTTAGGCAGGTCCA ACCGC-3'
	3'-GGGTG ACGTGGACAGTGGAGA       CCGGGTCGTGTTCCCGACGTACACCGGTG        TGGAATCCGTCCAGGT TGGCG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 002-241889867
Forward Primer: 5'-TCAGAGCCTGAAGCAGGAGC-3' T_m: 59.508
Reverse Primer: 5'-GGGCAAGTGGATGGGTGGAT-3' T_m: 59.508
Expected PCR Product Length: 287
Expected CelI Cleavage Product Sizes: 135, 152
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr2:241889970-241890030

Rank: 111	Homology Score: 10
	Genomic Region: Intergenic	Closest Gene: DHRS2
Match Type: R10R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TCTCC TTCACCTGACTCTAGT                 TGTTGCTGGA                 ACAGGACTTTGTTGTA TATTC-3'
	3'-AGAGG AAGTGGACTGAGATCA                 ACAACGACCT                 TGTCCTGAAACAACAT ATAAG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 014-024242484
Forward Primer: 5'-CAAGTCTTCCTCAGCCATCAGACA-3' T_m: 59.038
Reverse Primer: 5'-CCAGACACATCAACAGAGTGGAAG-3' T_m: 59.038
Expected PCR Product Length: 298
Expected CelI Cleavage Product Sizes: 138, 160
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr14:24242599-24242640

Rank: 112	Homology Score: 10
	Genomic Region: Intergenic	Closest Gene: EIF3B
Match Type: R23R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CTTTG TTCTCCCGTTTCCTGT          GTATGGTTCAGCCCCAGTGAGCC           ACAGGAGACAGGGCCA GGAGC-3'
	3'-GAAAC AAGAGGGCAAAGGACA          CATACCAAGTCGGGGTCACTCGG           TGTCCTCTGTCCCGGT CCTCG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 007-002427539
Forward Primer: 5'-TTGGCCAGGCTGGTCTCGAA-3' T_m: 59.508
Reverse Primer: 5'-CTTTGATGAACCCACAGGGCG-3' T_m: 59.378
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 136, 134
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr7:2427646-2427700

Rank: 113	Homology Score: 10
	Genomic Region: Intergenic	Closest Gene: DKFZp686K1684
Match Type: R11R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GAACT TTCACCTGACTCCTTA                TGCTTAGCTCA                 AACGGGGGCAGGTTCA AGCAG-3'
	3'-CTTGA AAGTGGACTGAGGAAT                ACGAATCGAGT                 TTGCCCCCGTCCAAGT TCGTC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 011-031965225
Forward Primer: 5'-CCAGCCTGAGACAACCATCTTTAC-3' T_m: 59.038
Reverse Primer: 5'-GAAGGGCCATAACTTGGGCATTAG-3' T_m: 59.038
Expected PCR Product Length: 355
Expected CelI Cleavage Product Sizes: 192, 163
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr11:31965394-31965436

Rank: 114	Homology Score: 10
	Genomic Region: Intergenic	Closest Gene: NUBPL
Match Type: R13R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CTTTT TGCCCCTGAGTTCTGT               GGAAATCTTGGAA                ACAGGGCCCAGGAACA TATTC-3'
	3'-GAAAA ACGGGGACTCAAGACA               CCTTTAGAACCTT                TGTCCCGGGTCCTTGT ATAAG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 014-032435668
Forward Primer: 5'-GCAGTAAGCTTGTTTCCTTGGCC-3' T_m: 59.148
Reverse Primer: 5'-AACATGGGCTTCTTGCAAGGAGAG-3' T_m: 59.038
Expected PCR Product Length: 366
Expected CelI Cleavage Product Sizes: 235, 131
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr14:32435879-32435923

Rank: 115	Homology Score: 10
	Genomic Region: Intergenic	Closest Gene: FAM47A
Match Type: R10R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-AGTCT TCACCCTTACTCCTAT                 GCTTATTCTA                 ACAGGAGTTAGATGTA CTTCT-3'
	3'-TCAGA AGTGGGAATGAGGATA                 CGAATAAGAT                 TGTCCTCAATCTACAT GAAGA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 00X-034076224
Forward Primer: 5'-AGTATTGCCCTTTCTGCTAGGGAC-3' T_m: 59.038
Reverse Primer: 5'-CACTGCACACTTCTGTGGCTC-3' T_m: 59.378
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 134, 136
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chrX:34076335-34076376

Rank: 116	Homology Score: 10
	Genomic Region: Intergenic	Closest Gene: C1orf94
Match Type: R12R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GAGCC TGGATCAGCCTCCTGG                ATCAGCCAACCC                ACAGCAGTCAGGAACA TATCA-3'
	3'-CTCGG ACCTAGTCGGAGGACC                TAGTCGGTTGGG                TGTCGTCAGTCCTTGT ATAGT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 001-034895940
Forward Primer: 5'-TTACCTGGGAGGTGGAAACATGG-3' T_m: 59.148
Reverse Primer: 5'-CCTTCCTTGCTTTGTTGGTGGATG-3' T_m: 59.038
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 135, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr1:34896051-34896094

Rank: 117	Homology Score: 10
	Genomic Region: Intergenic	Closest Gene: LINC00310
Match Type: R26R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CAGAA TGCACTTGACGTCGGG         TAGCACAGCAGGGTGCGGTAATGGTG         ACAAGAATCAGCTGCA GTGTG-3'
	3'-GTCTT ACGTGAACTGCAGCCC         ATCGTGTCGTCCCACGCCATTACCAC         TGTTCTTAGTCGACGT CACAC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 021-035539617
Forward Primer: 5'-GGCCTTCCTCACTATCTTACAGTC-3' T_m: 59.038
Reverse Primer: 5'-GCAAAACCGTGTCTGCCCAAATC-3' T_m: 59.148
Expected PCR Product Length: 303
Expected CelI Cleavage Product Sizes: 144, 159
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr21:35539730-35539787

Rank: 118	Homology Score: 10
	Genomic Region: Intergenic	Closest Gene: TRAPPC3
Match Type: R11R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TCCCC TGCACCTGCCTTCTCT                GGGGACAGGCC                 ACAGCCATCCAGTGCA AGGCT-3'
	3'-AGGGG ACGTGGACGGAAGAGA                CCCCTGTCCGG                 TGTCGGTAGGTCACGT TCCGA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 001-036588039
Forward Primer: 5'-GCACATGTGTGTCCACATATGGC-3' T_m: 59.148
Reverse Primer: 5'-GGGCAGAGTTTAAGGAGAAAGGTG-3' T_m: 59.038
Expected PCR Product Length: 273
Expected CelI Cleavage Product Sizes: 140, 133
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr1:36588156-36588198

Rank: 119	Homology Score: 10
	Genomic Region: Intergenic	Closest Gene: ACTR3BP5
Match Type: R13R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-AATCA TCTAACGGACTCATGT               GGAATCATCATCA                AAAGGAGTCGAGTGCA ATCAT-3'
	3'-TTAGT AGATTGCCTGAGTACA               CCTTAGTAGTAGT                TTTCCTCAGCTCACGT TAGTA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Warning! Primer pair was generated using suboptimal parameters. 
Primer Index: 010-038882221
Forward Primer: 5'-GCAATCATTGAATGGACTGGAAAGGAATCATC-3' T_m: 59.848
Reverse Primer: 5'-CCATGCAATGATGATTCCATTCGATTCCATTC-3' T_m: 59.848
Expected PCR Product Length: 336
Expected CelI Cleavage Product Sizes: 152, 184
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr10:38882349-38882393

Rank: 120	Homology Score: 10
	Genomic Region: Intergenic	Closest Gene: DAB2
Match Type: R15R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-AGAGT TGCACCTATCTGCTGT              CTATTAGAGACAAAC               TCAAGAGTCAAAGGCA CAAAT-3'
	3'-TCTCA ACGTGGATAGACGACA              GATAATCTCTGTTTG               AGTTCTCAGTTTCCGT GTTTA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 005-039782571
Forward Primer: 5'-GTTAAAGACGTGAGAGAGAAAACCAACAGC-3' T_m: 60.058
Reverse Primer: 5'-CTGAGGAACTATTCTGAGGGGC-3' T_m: 59.258
Expected PCR Product Length: 364
Expected CelI Cleavage Product Sizes: 132, 232
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr5:39782678-39782724

Rank: 121	Homology Score: 10
	Genomic Region: Intergenic	Closest Gene: PTPRT
Match Type: R27R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GGGTC TGCACCAGGCCACTCT        CCTTTCTGAGCACCGAGTGAAGGCATC         ACAGGATCCAGTTGCA GAGCA-3'
	3'-CCCAG ACGTGGTCCGGTGAGA        GGAAAGACTCGTGGCTCACTTCCGTAG         TGTCCTAGGTCAACGT CTCGT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 020-040489952
Forward Primer: 5'-GTGCCACCCACACAGCATTC-3' T_m: 59.508
Reverse Primer: 5'-GCCAGCAATGTAGAGGGACAATG-3' T_m: 59.148
Expected PCR Product Length: 313
Expected CelI Cleavage Product Sizes: 182, 131
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr20:40490103-40490161

Rank: 122	Homology Score: 10
	Genomic Region: Intergenic	Closest Gene: GRXCR1
Match Type: R25R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TTGTA TCCACCTGGCAGCTGG         TATCATGTAGCACCGCTTATCTTGC          AAAAGAGTTAGGTGCA TGGGT-3'
	3'-AACAT AGGTGGACCGTCGACC         ATAGTACATCGTGGCGAATAGAACG          TTTTCTCAATCCACGT ACCCA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 004-043460367
Forward Primer: 5'-GCTAAGAGCCTCTTGGGCTTTTG-3' T_m: 59.148
Reverse Primer: 5'-AGCAGAGGCAGAAAGGACCAG-3' T_m: 59.378
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 136, 134
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr4:43460473-43460529

Rank: 123	Homology Score: 10
	Genomic Region: Intergenic	Closest Gene: WFDC3
Match Type: R19R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TTTCC TTCCTCTGATTACTGT            AAAAAACAGTGTTACATAA             ACAAGATTCAAGTGCA ATTTT-3'
	3'-AAAGG AAGGAGACTAATGACA            TTTTTTGTCACAATGTATT             TGTTCTAAGTTCACGT TAAAA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 020-044380819
Forward Primer: 5'-GCTTCCAGGAATTACTCCTGTGC-3' T_m: 59.148
Reverse Primer: 5'-GGCAATTACTCAAGTGCCCAGAAG-3' T_m: 59.038
Expected PCR Product Length: 355
Expected CelI Cleavage Product Sizes: 181, 174
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr20:44380973-44381023

Rank: 124	Homology Score: 10
	Genomic Region: Intergenic	Closest Gene: GNPDA2
Match Type: R15R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GTGGA TGCACCTGAATTGTGT              TCTGAGCTGGGAAAT               CCAGGAGTGACCTGGA GATTC-3'
	3'-CACCT ACGTGGACTTAACACA              AGACTCGACCCTTTA               GGTCCTCACTGGACCT CTAAG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 004-044831243
Forward Primer: 5'-GCCACTATGTTTGCTTCCCAACTC-3' T_m: 59.038
Reverse Primer: 5'-CTCTACCTGGCAGCCTTCATTG-3' T_m: 59.258
Expected PCR Product Length: 284
Expected CelI Cleavage Product Sizes: 152, 132
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr4:44831370-44831416

Rank: 125	Homology Score: 10
	Genomic Region: Intergenic	Closest Gene: LOC100216001
Match Type: R14R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CTTTT TGCAACTGGCTTCTGT               TACTGGTCCACACG               GCAGAAGTCAAGGCCA CCCAG-3'
	3'-GAAAA ACGTTGACCGAAGACA               ATGACCAGGTGTGC               CGTCTTCAGTTCCGGT GGGTC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 010-004489187
Forward Primer: 5'-GGTAGCCTGCACTCATGATTCTG-3' T_m: 59.148
Reverse Primer: 5'-CTCACAGCACCTATGACGGTTC-3' T_m: 59.258
Expected PCR Product Length: 444
Expected CelI Cleavage Product Sizes: 263, 181
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr10:4489425-4489470

Rank: 126	Homology Score: 10
	Genomic Region: Intergenic	Closest Gene: GOSR2
Match Type: R16R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GAGGC TGCAACTGACTGCTGC              CCCTGGGGAAGGTGGG              ACGGGGGCCAGATTCA CAGGG-3'
	3'-CTCCG ACGTTGACTGACGACG              GGGACCCCTTCCACCC              TGCCCCCGGTCTAAGT GTCCC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 017-045024202
Forward Primer: 5'-CTGGGTGACGAAATGAGACTCTG-3' T_m: 59.148
Reverse Primer: 5'-TTCATGTCAGGGTCCCCTGG-3' T_m: 59.508
Expected PCR Product Length: 308
Expected CelI Cleavage Product Sizes: 161, 147
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr17:45024337-45024384

Rank: 127	Homology Score: 10
	Genomic Region: Intergenic	Closest Gene: SPERT
Match Type: R30R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CACAG TGAACCTGATTCCTAT       AGATTTCTCGGTGGCAGGGCAAGAAGGAAC       AGAGCAGGCACATGCA ATGAA-3'
	3'-GTGTC ACTTGGACTAAGGATA       TCTAAAGAGCCACCGTCCCGTTCTTCCTTG       TCTCGTCCGTGTACGT TACTT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 013-046233753
Forward Primer: 5'-CTCCCTATGTTGTCCAGGCTAGA-3' T_m: 59.148
Reverse Primer: 5'-GCTGGGTTTGGTAGTTCTAACTCC-3' T_m: 59.038
Expected PCR Product Length: 362
Expected CelI Cleavage Product Sizes: 188, 174
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr13:46233908-46233969

Rank: 128	Homology Score: 10
	Genomic Region: Intergenic	Closest Gene: SSBP3
Match Type: R10R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TCGTC TGTGCCTGACTCCTGC                 TCCAAGGAGC                 ACAGGTATCGGATGGA TATCC-3'
	3'-AGCAG ACACGGACTGAGGACG                 AGGTTCCTCG                 TGTCCATAGCCTACCT ATAGG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 001-054689628
Forward Primer: 5'-GTGAAGGCAACTGCCAGGCT-3' T_m: 59.508
Reverse Primer: 5'-GGCTTTTGCTGAGTACAGACACC-3' T_m: 59.148
Expected PCR Product Length: 313
Expected CelI Cleavage Product Sizes: 156, 157
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr1:54689761-54689802

Rank: 129	Homology Score: 10
	Genomic Region: Intergenic	Closest Gene: GOT2
Match Type: R11R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CTGCC TGCACCCCACTCCTGC                TTGTCCAAATC                 AAATGAGACAGGCTCA ATTAA-3'
	3'-GACGG ACGTGGGGTGAGGACG                AACAGGTTTAG                 TTTACTCTGTCCGAGT TAATT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 016-059193695
Forward Primer: 5'-GTAGCCTCTCCCAATACTTAACGG-3' T_m: 59.038
Reverse Primer: 5'-CCTGTGTTGTTTCTGTCCAAGGC-3' T_m: 59.148
Expected PCR Product Length: 332
Expected CelI Cleavage Product Sizes: 130, 202
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr16:59193802-59193844

Rank: 130	Homology Score: 10
	Genomic Region: Intergenic	Closest Gene: FOXB1
Match Type: R15R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TGTTA TGCAGCTAACAGTTGT              TCTTCACTTCCTGGC               ACAGGAGACAGGAACA TCTTT-3'
	3'-ACAAT ACGTCGATTGTCAACA              AGAAGTGAAGGACCG               TGTCCTCTGTCCTTGT AGAAA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 015-060382268
Forward Primer: 5'-GAGAACTCAGTGATGTGGGAGATG-3' T_m: 59.038
Reverse Primer: 5'-CCTCCCCAAATACGCCACTTTAG-3' T_m: 59.148
Expected PCR Product Length: 367
Expected CelI Cleavage Product Sizes: 209, 158
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr15:60382452-60382498

Rank: 131	Homology Score: 10
	Genomic Region: Intergenic	Closest Gene: NFIA
Match Type: R12R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TGTAG TGCTCCTTGCACCTGA                CCACTTGACCTT                CCAGCAGACAGGTGCA CTGGA-3'
	3'-ACATC ACGAGGAACGTGGACT                GGTGAACTGGAA                GGTCGTCTGTCCACGT GACCT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 001-061258016
Forward Primer: 5'-CCAGTAGTTCCATCAAACTAGGGC-3' T_m: 59.038
Reverse Primer: 5'-GTCCTGTTCTCTGCTGCAGTTG-3' T_m: 59.258
Expected PCR Product Length: 308
Expected CelI Cleavage Product Sizes: 171, 137
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr1:61258163-61258206

Rank: 132	Homology Score: 10
	Genomic Region: Intergenic	Closest Gene: MALAT1
Match Type: R14R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GAGCT TGCATCATCCTCCTGA               CCAGGGTGGAATAA               CCAGGAGGCAGGCGCA CGTGA-3'
	3'-CTCGA ACGTAGTAGGAGGACT               GGTCCCACCTTATT               GGTCCTCCGTCCGCGT GCACT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 011-065255104
Forward Primer: 5'-TGGGGGAGAAAGGGAAAGGC-3' T_m: 59.508
Reverse Primer: 5'-AGGCGGAGGCAGAGTAGGTA-3' T_m: 59.508
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 135, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr11:65255214-65255259

Rank: 133	Homology Score: 10
	Genomic Region: Intergenic	Closest Gene: PTGER4P2
Match Type: R18R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GGTAA TCCACCTGCCTCGGCT             TCCCAAAGTGTTGGGGTT             ACAGGTGTGAGGTACA ACGCC-3'
	3'-CCATT AGGTGGACGGAGCCGA             AGGGTTTCACAACCCCAA             TGTCCACACTCCATGT TGCGG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 009-066282880
Forward Primer: 5'-ACTACAGGTGCACACCACCATG-3' T_m: 59.258
Reverse Primer: 5'-GCCTTCCCCAAGTTTGCATTTTCG-3' T_m: 59.038
Expected PCR Product Length: 301
Expected CelI Cleavage Product Sizes: 131, 170
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr9:66282984-66283033

Rank: 134	Homology Score: 10
	Genomic Region: Intergenic	Closest Gene: FAM86C2P
Match Type: R29R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TTTGG TGCACCCGACTGCCGT       GGGGTGTCCCAGCAAAAGATCATCAACGT        ACTGGAGCAAAATGCA GTCTA-3'
	3'-AAACC ACGTGGGCTGACGGCA       CCCCACAGGGTCGTTTTCTAGTAGTTGCA        TGACCTCGTTTTACGT CAGAT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 011-067617612
Forward Primer: 5'-GCTCTCCCCCTTTCGGATAG-3' T_m: 59.508
Reverse Primer: 5'-GTACACTTGGACCGGGCTTC-3' T_m: 59.508
Expected PCR Product Length: 289
Expected CelI Cleavage Product Sizes: 157, 132
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr11:67617737-67617797

Rank: 135	Homology Score: 10
	Genomic Region: Intergenic	Closest Gene: KCNJ2
Match Type: R21R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TATGC TACATGTGACTCCTAA           TTAAGTGATCTTTCCAGAACA            CCAAGAGTCAAGTGCA GTCGG-3'
	3'-ATACG ATGTACACTGAGGATT           AATTCACTAGAAAGGTCTTGT            GGTTCTCAGTTCACGT CAGCC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Warning! Primer pair was generated using suboptimal parameters. 
Primer Index: 017-068268613
Forward Primer: 5'-AGGAAGGGATTCTCCTAATAAATTGGCAGATG-3' T_m: 59.848
Reverse Primer: 5'-CTATGGTCCATGTCTAACGTTAATACTACATGC-3' T_m: 59.758
Expected PCR Product Length: 391
Expected CelI Cleavage Product Sizes: 130, 261
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr17:68268715-68268767

Rank: 136	Homology Score: 10
	Genomic Region: Intergenic	Closest Gene: MIR4278
Match Type: R18R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-ATGCT TAGACTGGAGTCCTGT             GTTTTGGGAAGGAGGACC             ACAGAGGTGAGGTGCA GTTTT-3'
	3'-TACGA ATCTGACCTCAGGACA             CAAAACCCTTCCTCCTGG             TGTCTCCACTCCACGT CAAAA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 005-006875444
Forward Primer: 5'-CTGTGGCAGTTACTCAGATTTCCC-3' T_m: 59.038
Reverse Primer: 5'-GCAGAAACCTGACAAACAGGACC-3' T_m: 59.148
Expected PCR Product Length: 275
Expected CelI Cleavage Product Sizes: 145, 130
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr5:6875562-6875611

Rank: 137	Homology Score: 10
	Genomic Region: Intergenic	Closest Gene: OR7E91P
Match Type: R29R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TAGGC TGCATGTTGCTCCAGT       AGGTTGATGACCTTCTGCTGGGACACCCC        ACGGCAGTCGGGTGCA CCAAG-3'
	3'-ATCCG ACGTACAACGAGGTCA       TCCAACTACTGGAAGACGACCCTGTGGGG        TGCCGTCAGCCCACGT GGTTC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 002-071249464
Forward Primer: 5'-TAGTGTACACTTGGACCGGGC-3' T_m: 59.378
Reverse Primer: 5'-ATCCCAAGTAAGGTACCTGCTGTC-3' T_m: 59.038
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 139, 131
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr2:71249571-71249631

Rank: 138	Homology Score: 10
	Genomic Region: Intergenic	Closest Gene: MIR4313
Match Type: R15R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CCACC TGCACCTGGCTCATCT              GGTGGCCTTGGCCTG               GAAGGAGCCAGAGGCA GAGGC-3'
	3'-GGTGG ACGTGGACCGAGTAGA              CCACCGGAACCGGAC               CTTCCTCGGTCTCCGT CTCCG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 015-076078165
Forward Primer: 5'-TACTCACCTCTTGCCTTCAAGTGG-3' T_m: 59.038
Reverse Primer: 5'-CTTTTCTTGGCCCTTGCCAGGA-3' T_m: 59.258
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 133, 137
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr15:76078273-76078319

Rank: 139	Homology Score: 10
	Genomic Region: Intergenic	Closest Gene: SALL3
Match Type: R16R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CACTC TGCACCTGCAGCCTCC              TCCTGATGGATGACCA              ACAGGAGCAAGGGGCA AGGCA-3'
	3'-GTGAG ACGTGGACGTCGGAGG              AGGACTACCTACTGGT              TGTCCTCGTTCCCCGT TCCGT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 018-076246512
Forward Primer: 5'-AGCCTCCTACTGATGGGTGG-3' T_m: 59.508
Reverse Primer: 5'-GCTGATGAAGAAGAGGATGGGG-3' T_m: 59.258
Expected PCR Product Length: 286
Expected CelI Cleavage Product Sizes: 151, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr18:76246637-76246684

Rank: 140	Homology Score: 10
	Genomic Region: Intergenic	Closest Gene: SALL3
Match Type: R10R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GCACC TGCAGCCTCCTCCTGA                 TGGATGACCA                 ACAGGAGCAAGGGGCA AGGCA-3'
	3'-CGTGG ACGTCGGAGGAGGACT                 ACCTACTGGT                 TGTCCTCGTTCCCCGT TCCGT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 018-076246512
Forward Primer: 5'-AGCCTCCTACTGATGGGTGG-3' T_m: 59.508
Reverse Primer: 5'-GCTGATGAAGAAGAGGATGGGG-3' T_m: 59.258
Expected PCR Product Length: 286
Expected CelI Cleavage Product Sizes: 154, 132
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr18:76246643-76246684

Rank: 141	Homology Score: 10
	Genomic Region: Intergenic	Closest Gene: EDIL3
Match Type: R12R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GTCCA TGTACCTAACTCCTTT                GTCTTCAGCATG                AGAGAACACAGGGGCA TACTG-3'
	3'-CAGGT ACATGGATTGAGGAAA                CAGAAGTCGTAC                TCTCTTGTGTCCCCGT ATGAC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 005-083704636
Forward Primer: 5'-GGAACGGATTAAAGGGAAGCCAG-3' T_m: 59.148
Reverse Primer: 5'-GGTCTGTTTCTGTAACATCAGAGGCAAAG-3' T_m: 60.178
Expected PCR Product Length: 361
Expected CelI Cleavage Product Sizes: 133, 228
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr5:83704745-83704788

Rank: 142	Homology Score: 10
	Genomic Region: Intergenic	Closest Gene: CPXCR1
Match Type: R16R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TGATG TGTGCATGACTCTTGG              CCCAATGTTGAGTAAC              ACAGGAGTTAATTGCA TGGAC-3'
	3'-ACTAC ACACGTACTGAGAACC              GGGTTACAACTCATTG              TGTCCTCAATTAACGT ACCTG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Warning! Primer pair was generated using suboptimal parameters. 
Primer Index: 00X-088338909
Forward Primer: 5'-CCCCCCCAGAAAAGTGGTTTATG-3' T_m: 59.148
Reverse Primer: 5'-CAGATTTGGCTCACAGTAGCATACTTTACTC-3' T_m: 59.958
Expected PCR Product Length: 415
Expected CelI Cleavage Product Sizes: 197, 218
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chrX:88339080-88339127

Rank: 143	Homology Score: 10
	Genomic Region: Intergenic	Closest Gene: MIR4291
Match Type: R22R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-AGAGC TGCACTTCACTCCTGA           AGCCAGCGAGACCATGAACTCA           CCAGGAGTAAGGAAGA ACTCC-3'
	3'-TCTCG ACGTGAAGTGAGGACT           TCGGTCGCTCTGGTACTTGAGT           GGTCCTCATTCCTTCT TGAGG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 009-096644130
Forward Primer: 5'-CCCCCTTACATAGTGTGGAAGC-3' T_m: 59.258
Reverse Primer: 5'-TCGGAGTTTCTTCCTTCTGGTGG-3' T_m: 59.148
Expected PCR Product Length: 273
Expected CelI Cleavage Product Sizes: 143, 130
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr9:96644244-96644297

Rank: 144	Homology Score: 8
	Genomic Region: Exon	Closest Gene: HCFC2
Match Type: R30R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GCAGA TGTACCTGACTACAGC       TTGCTTAAGAAACAAGATCTTGTTCCAGGC       ACAGGATACAGATTCA GGGTT-3'
	3'-CGTCT ACATGGACTGATGTCG       AACGAATTCTTTGTTCTAGAACAAGGTCCG       TGTCCTATGTCTAAGT CCCAA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 012-104495588
Forward Primer: 5'-CATTCCATGTGAGAGAGAGGGAG-3' T_m: 59.148
Reverse Primer: 5'-TCTGACTGCAGAAGGAGCTCCA-3' T_m: 59.258
Expected PCR Product Length: 335
Expected CelI Cleavage Product Sizes: 205, 130
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr12:104495760-104495821

Rank: 145	Homology Score: 8
	Genomic Region: Intron	Closest Gene: ARHGAP27
Match Type: R14R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TACCC TCCTCCTTGCTCCTGT               TCATGGAGGCCCAG               ACAAGACCCAGATGCA GCTCA-3'
	3'-ATGGG AGGAGGAACGAGGACA               AGTACCTCCGGGTC               TGTTCTGGGTCTACGT CGAGT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: ap1-000102177
Forward Primer: 5'-AGATGCTCTCCTCTCCTGGC-3' T_m: 59.508
Reverse Primer: 5'-TGAGAGCCAGGTCCCTCTTG-3' T_m: 59.508
Expected PCR Product Length: 283
Expected CelI Cleavage Product Sizes: 147, 136
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr17_ctg5_hap1:102299-102344

Rank: 146	Homology Score: 8
	Genomic Region: Intron	Closest Gene: ATP2B2
Match Type: R19R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GGACA TGCAGCTGACACTGGT            ACTTTCTCCTCTTCTGGAC             AAGGGAGTGAGGTGTA GCCAG-3'
	3'-CCTGT ACGTCGACTGTGACCA            TGAAAGAGGAGAAGACCTG             TTCCCTCACTCCACAT CGGTC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 003-010453381
Forward Primer: 5'-TGTTGCAGACACTGGAACAGAGC-3' T_m: 59.148
Reverse Primer: 5'-CGTGAGAGTGGACTTGCAAGTCTG-3' T_m: 60.908
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 135, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr3:10453489-10453539

Rank: 147	Homology Score: 8
	Genomic Region: Intron	Closest Gene: MYBPHL
Match Type: R30R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-ATGTT TTCACATGCTTCCTGT       GATACTCAGTCCCAGAGCTCTCTGTAAGGA       AGAGGAGTTAGGAGGA AGAAA-3'
	3'-TACAA AAGTGTACGAAGGACA       CTATGAGTCAGGGTCTCGAGAGACATTCCT       TCTCCTCAATCCTCCT TCTTT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 001-109847447
Forward Primer: 5'-GCAGGCTTGCCAAACTAAAAGCTC-3' T_m: 59.038
Reverse Primer: 5'-TCAGAGAGCCAACAGAGCCC-3' T_m: 59.508
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 133, 137
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr1:109847547-109847608

Rank: 148	Homology Score: 8
	Genomic Region: Intron	Closest Gene: C1orf127
Match Type: R23R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GCATC TGCAGCTACCACCTGT          CTCCCTGGGCCACGTCCTGTGGT           GCAGGAGCCAGGGCCA CCTGC-3'
	3'-CGTAG ACGTCGATGGTGGACA          GAGGGACCCGGTGCAGGACACCA           CGTCCTCGGTCCCGGT GGACG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 001-011023947
Forward Primer: 5'-TTTAATCCCCCCTCCCCTCC-3' T_m: 59.508
Reverse Primer: 5'-AAAGCGTCCACTGTGGGCCCAT-3' T_m: 61.288
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 137, 133
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr1:11024055-11024109

Rank: 149	Homology Score: 8
	Genomic Region: Intron	Closest Gene: TAG
Match Type: R29R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TTTTT TGCATTTTACACCTGT       GTGACTTTTATTTTATTTTATTTTTAGTT        CCAGGATACATGTGCA GGATG-3'
	3'-AAAAA ACGTAAAATGTGGACA       CACTGAAAATAAAATAAAATAAAAATCAA        GGTCCTATGTACACGT CCTAC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 005-012711008
Forward Primer: 5'-GGTCATTCTTGCTCAGTTTGCCC-3' T_m: 59.148
Reverse Primer: 5'-GGGAGGGAGAGCATCAGGAA-3' T_m: 59.508
Expected PCR Product Length: 439
Expected CelI Cleavage Product Sizes: 291, 148
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr5:12711267-12711327

Rank: 150	Homology Score: 8
	Genomic Region: Intron	Closest Gene: MGMT
Match Type: R30R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-ACACA TCCACGTAACTCTTGT       CCTTAGCAGTAGTGAGGGGCTTCTGCAGGC       AGAGGAGTCGGCTCCA CGGTC-3'
	3'-TGTGT AGGTGCATTGAGAACA       GGAATCGTCATCACTCCCCGAAGACGTCCG       TCTCCTCAGCCGAGGT GCCAG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 010-131523914
Forward Primer: 5'-CTCGGAGGTCTGTCCATGCT-3' T_m: 59.508
Reverse Primer: 5'-GAGCTAGAAGGGGCTGGTTG-3' T_m: 59.508
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 135, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr10:131524016-131524077

Rank: 151	Homology Score: 8
	Genomic Region: Intron	Closest Gene: RRN3
Match Type: R10R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GTTAG TGAAGCTGAGTGCTGT                 TTTCAGGTGC                 ACAGTAGTCATCTGTA GTTTT-3'
	3'-CAATC ACTTCGACTCACGACA                 AAAGTCCACG                 TGTCATCAGTAGACAT CAAAA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 016-015162374
Forward Primer: 5'-GCAGGCTGTAAGATTCCACGAC-3' T_m: 59.258
Reverse Primer: 5'-GTACAAGTTGGTCAGCTTGCCTC-3' T_m: 59.148
Expected PCR Product Length: 372
Expected CelI Cleavage Product Sizes: 190, 182
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr16:15162541-15162582

Rank: 152	Homology Score: 8
	Genomic Region: Intron	Closest Gene: RPL18A
Match Type: R30R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TAGGG TGGCCCTGCCTCCTGC       TTCCCGAATCTGTGGTCACTAGATGTAGGA       AAGGGAGTGAGGTGGA TCTCC-3'
	3'-ATCCC ACCGGGACGGAGGACG       AAGGGCTTAGACACCAGTGATCTACATCCT       TTCCCTCACTCCACCT AGAGG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 019-017971876
Forward Primer: 5'-CTCAATTAATCCTCCCTGGAGACC-3' T_m: 59.038
Reverse Primer: 5'-ATTCGCATGCGGTAGAGGGG-3' T_m: 59.508
Expected PCR Product Length: 303
Expected CelI Cleavage Product Sizes: 143, 160
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr19:17971986-17972047

Rank: 153	Homology Score: 8
	Genomic Region: Intron	Closest Gene: HTR3D
Match Type: R17R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TTCTC TGCTCCTGCCTCCTTC             CCTGTCTCCCTCCCTCC              ACAGGTGACATTTGCA GCCCA-3'
	3'-AAGAG ACGAGGACGGAGGAAG             GGACAGAGGGAGGGAGG              TGTCCACTGTAAACGT CGGGT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 003-183756342
Forward Primer: 5'-AGGGAGATGCTGTCCCACTG-3' T_m: 59.508
Reverse Primer: 5'-TCCATTCTGAGCCCCCTGTC-3' T_m: 59.508
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 139, 131
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr3:183756455-183756503

Rank: 154	Homology Score: 8
	Genomic Region: Intron	Closest Gene: HTR3E
Match Type: R17R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TTCTC TGCTCCTGCCTCCTTC             CCTGTCTCCCTCCCTCC              ACAGGTGACATTTGCA GCCCA-3'
	3'-AAGAG ACGAGGACGGAGGAAG             GGACAGAGGGAGGGAGG              TGTCCACTGTAAACGT CGGGT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 003-183824037
Forward Primer: 5'-CCTGCTGCTCCACTGCAACA-3' T_m: 59.508
Reverse Primer: 5'-TCCATTCTGAGCCCCCTGTC-3' T_m: 59.508
Expected PCR Product Length: 293
Expected CelI Cleavage Product Sizes: 162, 131
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr3:183824173-183824221

Rank: 155	Homology Score: 8
	Genomic Region: Intron	Closest Gene: PAX7
Match Type: R21R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-ATGCA TGCTCCTCATTCATGT           CATTCTCTAAGTTATTTGTCC            CCAGGTTGCAGGTGCA AAAAC-3'
	3'-TACGT ACGAGGAGTAAGTACA           GTAAGAGATTCAATAAACAGG            GGTCCAACGTCCACGT TTTTG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 001-019065509
Forward Primer: 5'-AGAGTCCCATGTCCTGGACTG-3' T_m: 59.378
Reverse Primer: 5'-TGCTCAGTGGCCTCCACAAATC-3' T_m: 59.258
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 135, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr1:19065616-19065668

Rank: 156	Homology Score: 8
	Genomic Region: Intron	Closest Gene: VASH2
Match Type: R12R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CATTT TTCACCAGACTCCCGG                CTCTTCAGCCTG                AGAGGAATATGGTGCA GAGAA-3'
	3'-GTAAA AAGTGGTCTGAGGGCC                GAGAAGTCGGAC                TCTCCTTATACCACGT CTCTT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 001-213140398
Forward Primer: 5'-ATTCTAAGAGTGGCGTGTGGAGG-3' T_m: 59.148
Reverse Primer: 5'-GTTGTGGGTGATTCCAATGGCATG-3' T_m: 59.038
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 134, 136
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr1:213140508-213140551

Rank: 157	Homology Score: 8
	Genomic Region: Intron	Closest Gene: RRN3P1
Match Type: R10R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GTTAG TGAAGCTGAGTGCTGT                 TTTCAGGTGC                 ACAGTAGTCATCTGTA GTTTT-3'
	3'-CAATC ACTTCGACTCACGACA                 AAAGTCCACG                 TGTCATCAGTAGACAT CAAAA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 016-021809420
Forward Primer: 5'-GGCTGTAAGATTCCACGACAACG-3' T_m: 59.148
Reverse Primer: 5'-GTACAAGTTGGTCAGCTTGCCTC-3' T_m: 59.148
Expected PCR Product Length: 370
Expected CelI Cleavage Product Sizes: 188, 182
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr16:21809585-21809626

Rank: 158	Homology Score: 8
	Genomic Region: Intron	Closest Gene: RRN3P3
Match Type: R10R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GTTAG TGAAGCTGAGTGCTGT                 TTTCAGGTGC                 ACAGTAGTCATCTGTA GTTTT-3'
	3'-CAATC ACTTCGACTCACGACA                 AAAGTCCACG                 TGTCATCAGTAGACAT CAAAA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 016-022444397
Forward Primer: 5'-GGCTGTAAGATTCCACGACAACG-3' T_m: 59.148
Reverse Primer: 5'-GTACAAGTTGGTCAGCTTGCCTC-3' T_m: 59.148
Expected PCR Product Length: 370
Expected CelI Cleavage Product Sizes: 188, 182
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr16:22444562-22444603

Rank: 159	Homology Score: 8
	Genomic Region: Intron	Closest Gene: HDLBP
Match Type: R22R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-ATTTA TGCAGCCTACTCCTCT           CTTTGCTTTAACTTATCACCTG           ACAGGAACCCGCTGCA GTGGA-3'
	3'-TAAAT ACGTCGGATGAGGAGA           GAAACGAAATTGAATAGTGGAC           TGTCCTTGGGCGACGT CACCT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 002-242219169
Forward Primer: 5'-CACTTCTGGAGACGTACTTCAGG-3' T_m: 59.148
Reverse Primer: 5'-CTGCTGCCAAGGAAGTGGTG-3' T_m: 59.508
Expected PCR Product Length: 316
Expected CelI Cleavage Product Sizes: 183, 133
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr2:242219323-242219376

Rank: 160	Homology Score: 8
	Genomic Region: Intron	Closest Gene: LINC00032
Match Type: R26R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TATGA TGAACCTCACTGTTGT         TCAGTCAAGTCAGACTGAGGGTCTTG         ACATCAGTCATGAGCA AATGG-3'
	3'-ATACT ACTTGGAGTGACAACA         AGTCAGTTCAGTCTGACTCCCAGAAC         TGTAGTCAGTACTCGT TTACC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 009-027247215
Forward Primer: 5'-CAAGCACAGGCAGATGCTTTAGG-3' T_m: 59.148
Reverse Primer: 5'-CATCACGACTGTTGCCTGTTCTTG-3' T_m: 59.038
Expected PCR Product Length: 432
Expected CelI Cleavage Product Sizes: 133, 299
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr9:27247317-27247374

Rank: 161	Homology Score: 8
	Genomic Region: Intron	Closest Gene: RRN3P2
Match Type: R10R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-AAAAC TACAGATGACTACTGT                 GCACCTGAAA                 ACAGCACTCAGCTTCA CTAAC-3'
	3'-TTTTG ATGTCTACTGATGACA                 CGTGGACTTT                 TGTCGTGAGTCGAAGT GATTG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Warning! Primer pair was generated using suboptimal parameters. 
Primer Index: 016-029109731
Forward Primer: 5'-CTTGTTTTTGTAAGCAGTGACATCCCATTTAGG-3' T_m: 59.758
Reverse Primer: 5'-CCACGACAACACGAAGTTCATACG-3' T_m: 59.038
Expected PCR Product Length: 442
Expected CelI Cleavage Product Sizes: 270, 172
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr16:29109978-29110019

Rank: 162	Homology Score: 8
	Genomic Region: Intron	Closest Gene: APOL2
Match Type: R25R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-AGGCC TTCACCACACTCCTAT         GACATTGAATTGCTATTCCTATTGG          ACAGAAGACAGTTCCA AGTCT-3'
	3'-TCCGG AAGTGGTGTGAGGATA         CTGTAACTTAACGATAAGGATAACC          TGTCTTCTGTCAAGGT TCAGA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 022-036626609
Forward Primer: 5'-CTGACTCCAGGCTCTGACAC-3' T_m: 59.508
Reverse Primer: 5'-GCTGGGGCCTTTTTTCTCATCTG-3' T_m: 59.148
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 130, 140
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr22:36626709-36626765

Rank: 163	Homology Score: 8
	Genomic Region: Intron	Closest Gene: CPNE5
Match Type: R17R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-ACAGC TGCCCCTGACTGGGGT             AGGCAGGTGGGGCAGGA              AGAGGAGCCTGGGGCA GTGGT-3'
	3'-TGTCG ACGGGGACTGACCCCA             TCCGTCCACCCCGTCCT              TCTCCTCGGACCCCGT CACCA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 006-036744134
Forward Primer: 5'-TCTCTGCTCCTTCTCGCCCA-3' T_m: 59.508
Reverse Primer: 5'-TGGGGGGTGGTATTTGGAGC-3' T_m: 59.508
Expected PCR Product Length: 278
Expected CelI Cleavage Product Sizes: 144, 134
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr6:36744252-36744300

Rank: 164	Homology Score: 8
	Genomic Region: Intron	Closest Gene: TOX2
Match Type: R26R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-AGAGC TGCTCCTGAGTCATGA         GGGAGAATTAAGGGAAAATCGCAGCG         ACATTCGTCAGCTGCA GCCTC-3'
	3'-TCTCG ACGAGGACTCAGTACT         CCCTCTTAATTCCCTTTTAGCGTCGC         TGTAAGCAGTCGACGT CGGAG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 020-042646910
Forward Primer: 5'-GCTGGGCTCCTAATCAGAGCA-3' T_m: 59.378
Reverse Primer: 5'-AGATGGCTTCTCAGCAATGCCG-3' T_m: 59.258
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 135, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr20:42647014-42647071

Rank: 165	Homology Score: 8
	Genomic Region: Intron	Closest Gene: ARHGAP27
Match Type: R14R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TACCC TCCTCCTTGCTCCTGT               TCATGGAGGCCCAG               ACAAGACCCAGATGCA GCTCA-3'
	3'-ATGGG AGGAGGAACGAGGACA               AGTACCTCCGGGTC               TGTTCTGGGTCTACGT CGAGT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 017-043487041
Forward Primer: 5'-AGATGCTCTCCTCTCCTGGC-3' T_m: 59.508
Reverse Primer: 5'-TGAGAGCCAGGTCCCTCTTG-3' T_m: 59.508
Expected PCR Product Length: 283
Expected CelI Cleavage Product Sizes: 147, 136
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr17:43487163-43487208

Rank: 166	Homology Score: 8
	Genomic Region: Intron	Closest Gene: NPC1L1
Match Type: R21R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CCCTC TTCACCTCAGCCCTGT           GACATAGCCCCCAGGGCTTCT            GCAGGTGGCAGGTTCA TTCTG-3'
	3'-GGGAG AAGTGGAGTCGGGACA           CTGTATCGGGGGTCCCGAAGA            CGTCCACCGTCCAAGT AAGAC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 007-044570049
Forward Primer: 5'-GGAGATGGATGGTGGTGATAGTTG-3' T_m: 59.038
Reverse Primer: 5'-TTAGGCAGTACTGGCCTGGG-3' T_m: 59.508
Expected PCR Product Length: 434
Expected CelI Cleavage Product Sizes: 294, 140
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr7:44570315-44570367

Rank: 167	Homology Score: 8
	Genomic Region: Intron	Closest Gene: USP34
Match Type: R30R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TTTCT TACACCTGATTACTGC       CAATGATGGGTTTTGGCCTTTTACCAAGTA       CCATGACCCAGGTGCA TAATC-3'
	3'-AAAGA ATGTGGACTAATGACG       GTTACTACCCAAAACCGGAAAATGGTTCAT       GGTACTGGGTCCACGT ATTAG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 002-061428882
Forward Primer: 5'-GGTTAAAGCTGTCCGAGTAGCTAG-3' T_m: 59.038
Reverse Primer: 5'-GCTGGTTCCTGGTAACCTGG-3' T_m: 59.508
Expected PCR Product Length: 326
Expected CelI Cleavage Product Sizes: 190, 136
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr2:61429039-61429100

Rank: 168	Homology Score: 8
	Genomic Region: Intron	Closest Gene: TPD52L2
Match Type: R15R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GGAAG TGCACACGGCTCCAGT              CCTATGGGACATTTG               AAGGGAGTGAGATGCA GCCAG-3'
	3'-CCTTC ACGTGTGCCGAGGTCA              GGATACCCTGTAAAC               TTCCCTCACTCTACGT CGGTC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 020-062498891
Forward Primer: 5'-CCTGGGTGACAGAGTAAGACTCG-3' T_m: 61.088
Reverse Primer: 5'-CCAATATGGTGAAACCCCGTCTC-3' T_m: 59.148
Expected PCR Product Length: 307
Expected CelI Cleavage Product Sizes: 130, 177
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr20:62498996-62499042

Rank: 169	Homology Score: 8
	Genomic Region: Intron	Closest Gene: CYP7B1
Match Type: R17R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CTCTG TGCAGTTGAGTTCTGT             GCATGTCAAGTATGAAT              AGAGTAGTCATGTTCA AAGGC-3'
	3'-GAGAC ACGTCAACTCAAGACA             CGTACAGTTCATACTTA              TCTCATCAGTACAAGT TTCCG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 008-065585407
Forward Primer: 5'-CATCAATGAGTGAGCCCCTTTGG-3' T_m: 59.148
Reverse Primer: 5'-GGAGAAACTCCACAAGACTAGAGC-3' T_m: 59.038
Expected PCR Product Length: 272
Expected CelI Cleavage Product Sizes: 138, 134
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr8:65585519-65585567

Rank: 170	Homology Score: 8
	Genomic Region: Intron	Closest Gene: HOMER1
Match Type: R15R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-AAGGA TACCCCTAAATCCTGT              CATTCTCCCACTTAA               CCTGGATTCAGGTGAA GACCT-3'
	3'-TTCCT ATGGGGATTTAGGACA              GTAAGAGGGTGAATT               GGACCTAAGTCCACTT CTGGA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 005-078702362
Forward Primer: 5'-GAAAGGATCCTGGATGAGGGG-3' T_m: 59.378
Reverse Primer: 5'-GTAGGAGTTTCCTGCCTCTGTC-3' T_m: 59.258
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 132, 138
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr5:78702469-78702515

Rank: 171	Homology Score: 8
	Genomic Region: Intron	Closest Gene: KIAA1609
Match Type: R30R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CCGCT TCCCCCTGCTTCCTGT       GCCATGTCACCAAGCTGAAGCGAGGGCTGG       ACAGGAGCCACGGTCA CCCCA-3'
	3'-GGCGA AGGGGGACGAAGGACA       CGGTACAGTGGTTCGACTTCGCTCCCGACC       TGTCCTCGGTGCCAGT GGGGT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 016-084515616
Forward Primer: 5'-AGCTGAGCTTCGGCGTGGAT-3' T_m: 59.508
Reverse Primer: 5'-ATGGTGTCAGCGCCAGACCT-3' T_m: 59.508
Expected PCR Product Length: 282
Expected CelI Cleavage Product Sizes: 132, 150
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr16:84515715-84515776

Rank: 172	Homology Score: 8
	Genomic Region: Intron	Closest Gene: ZFPM1
Match Type: R29R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-ACGCC TTCACCTGCCTCCTCA       AGTCAGAGCTGTGAGCCAGCCTGCCAGGG        CCAGGGCTCAGGTGGA GAAAC-3'
	3'-TGCGG AAGTGGACGGAGGAGT       TCAGTCTCGACACTCGGTCGGACGGTCCC        GGTCCCGAGTCCACCT CTTTG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 016-088575852
Forward Primer: 5'-CCATTAGGCACACAGGGAGG-3' T_m: 59.508
Reverse Primer: 5'-CTGCCTCCTGAACCCACTCT-3' T_m: 59.508
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 135, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr16:88575955-88576015

Rank: 173	Homology Score: 8
	Genomic Region: Intron	Closest Gene: ASAP2
Match Type: R17R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CAGCT TCCACCTGGCCCCTGA             GAATTAATGAGGTCTGG              CCAGGCGGCAGCTGCA GTGCA-3'
	3'-GTCGA AGGTGGACCGGGGACT             CTTAATTACTCCAGACC              GGTCCGCCGTCGACGT CACGT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 002-009410094
Forward Primer: 5'-CCTCGGTAACTTGAGAGGAACCTA-3' T_m: 59.038
Reverse Primer: 5'-AAAACTCCACTCCAGCTCAGAGCG-3' T_m: 60.908
Expected PCR Product Length: 335
Expected CelI Cleavage Product Sizes: 196, 139
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr2:9410264-9410312

Rank: 174	Homology Score: 8
	Genomic Region: Intergenic	Closest Gene: NPAS2
Match Type: R19R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CAGAT TGCAGGTGATTGCTGT            CAGATGGCCAAGTCCTGCC             ACAAGAGCCAGTTCCA GGCAC-3'
	3'-GTCTA ACGTCCACTAACGACA            GTCTACCGGTTCAGGACGG             TGTTCTCGGTCAAGGT CCGTG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 002-101420981
Forward Primer: 5'-GAGATTTGGGTGGGAACACAGAG-3' T_m: 59.148
Reverse Primer: 5'-AAACACCAGAGGTTTGTGTGGGC-3' T_m: 59.148
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 135, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr2:101421089-101421139

Rank: 175	Homology Score: 8
	Genomic Region: Intergenic	Closest Gene: DAP
Match Type: R19R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GAGTA TGGCCCTGGTTCCTGT            CCTCAGCTTAGCATGGGAG             ACAGGAGGCAGGAATA CAAGC-3'
	3'-CTCAT ACCGGGACCAAGGACA            GGAGTCGAATCGTACCCTC             TGTCCTCCGTCCTTAT GTTCG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 005-010678604
Forward Primer: 5'-CCTGGGGGTGGTTCTGTTCCAT-3' T_m: 61.288
Reverse Primer: 5'-GAGCACCAGAGAGAGCACCA-3' T_m: 59.508
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 131, 139
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr5:10678708-10678758

Rank: 176	Homology Score: 8
	Genomic Region: Intergenic	Closest Gene: KLF4
Match Type: R19R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CTCCC TGCAGCTGATTCATGG            GAACTCCGAGCCTGCATTA             ACAGGAGGGAAGGGCA GCTTG-3'
	3'-GAGGG ACGTCGACTAAGTACC            CTTGAGGCTCGGACGTAAT             TGTCCTCCCTTCCCGT CGAAC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 009-110904790
Forward Primer: 5'-AAGCGGGTGGGGTAACAGTG-3' T_m: 59.508
Reverse Primer: 5'-CAGTGGAGGCTATTGTGTTTGTGC-3' T_m: 59.038
Expected PCR Product Length: 278
Expected CelI Cleavage Product Sizes: 133, 145
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr9:110904896-110904946

Rank: 177	Homology Score: 8
	Genomic Region: Intergenic	Closest Gene: NEIL2
Match Type: R11R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-AGTGA TGGAACTGAATCCTTT                CAAGAAATCCC                 ACAGAGGCCGGGTGCA GTGGC-3'
	3'-TCACT ACCTTGACTTAGGAAA                GTTCTTTAGGG                 TGTCTCCGGCCCACGT CACCG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 008-011624011
Forward Primer: 5'-CCCAATCCAGGAGGCAGACA-3' T_m: 59.508
Reverse Primer: 5'-CCACCACACCCTGCTAATTCTTG-3' T_m: 59.148
Expected PCR Product Length: 326
Expected CelI Cleavage Product Sizes: 165, 161
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr8:11624153-11624195

Rank: 178	Homology Score: 8
	Genomic Region: Intergenic	Closest Gene: SNTB1
Match Type: R25R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CTTCC TGCATCTGTTTCTTGT         CACACAGGTGGCTAAGAGCCTAGAC          TCAGGAGTCAGAATCA TTGGG-3'
	3'-GAAGG ACGTAGACAAAGAACA         GTGTGTCCACCGATTCTCGGATCTG          AGTCCTCAGTCTTAGT AACCC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 008-121929055
Forward Primer: 5'-ATTCCCCACATCACTGCACAAGC-3' T_m: 59.148
Reverse Primer: 5'-GCAGATACTGTGTGGGAAGCATTG-3' T_m: 59.038
Expected PCR Product Length: 337
Expected CelI Cleavage Product Sizes: 130, 207
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr8:121929155-121929211

Rank: 179	Homology Score: 8
	Genomic Region: Intergenic	Closest Gene: LHX6
Match Type: R21R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-AATTC TGCTCCTGAATTCTGA           TTAAGTGCTCAGATAATAAAA            ACTGGAGTAAGAGGCA GTAAA-3'
	3'-TTAAG ACGAGGACTTAAGACT           AATTCACGAGTCTATTATTTT            TGACCTCATTCTCCGT CATTT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 009-124993691
Forward Primer: 5'-GTGAAGCATGTGCTCCTAGAGTG-3' T_m: 59.148
Reverse Primer: 5'-GAAGAACTTGTCCAGGGTTACCCA-3' T_m: 59.038
Expected PCR Product Length: 402
Expected CelI Cleavage Product Sizes: 258, 144
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr9:124993921-124993973

Rank: 180	Homology Score: 8
	Genomic Region: Intergenic	Closest Gene: TEAD1
Match Type: R20R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-ATAAC TCCTCCCGACCCCTGT            CCTCAGCATCACCATGGGGT            TCATGATTCAGGTGTA TGCAG-3'
	3'-TATTG AGGAGGGCTGGGGACA            GGAGTCGTAGTGGTACCCCA            AGTACTAAGTCCACAT ACGTC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 011-012966658
Forward Primer: 5'-TGAGGGATAGAGCAGAGGATGG-3' T_m: 59.258
Reverse Primer: 5'-GGGGGAATGATGTCACTGGAAC-3' T_m: 59.258
Expected PCR Product Length: 295
Expected CelI Cleavage Product Sizes: 131, 164
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr11:12966761-12966812

Rank: 181	Homology Score: 8
	Genomic Region: Intergenic	Closest Gene: ARHGEF4
Match Type: R18R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GGCCT TGTCTCTGACTCCTCT             CTTCTTGCCCTCCCAGAC             TCCAGAGCCAGGTGCA CACCT-3'
	3'-CCGGA ACAGAGACTGAGGAGA             GAAGAACGGGAGGGTCTG             AGGTCTCGGTCCACGT GTGGA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 002-131671419
Forward Primer: 5'-ACCTCCCTTCCTAGGGGTAC-3' T_m: 59.508
Reverse Primer: 5'-ACAGCGTTTCAGAATCATCGCGG-3' T_m: 59.148
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 135, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr2:131671527-131671576

Rank: 182	Homology Score: 8
	Genomic Region: Intergenic	Closest Gene: LOC286083
Match Type: R22R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TGACT TGCACTGTACTCCTGC           TGAAGTGACCCGTCTCAAGATA           TCTGGACGCAGGTGCA CACCT-3'
	3'-ACTGA ACGTGACATGAGGACG           ACTTCACTGGGCAGAGTTCTAT           AGACCTGCGTCCACGT GTGGA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 008-001326103
Forward Primer: 5'-TTCACGCACCCCTGTGCAGA-3' T_m: 59.508
Reverse Primer: 5'-TGTGCAGGACACACAGGTGAG-3' T_m: 59.378
Expected PCR Product Length: 291
Expected CelI Cleavage Product Sizes: 143, 148
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr8:1326217-1326270

Rank: 183	Homology Score: 8
	Genomic Region: Intergenic	Closest Gene: PISRT1
Match Type: R11R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GTGAT TACCCCTGACTCCACT                CCTGGATGAGG                 TCAGGGGTCATCTGCA CAGAC-3'
	3'-CACTA ATGGGGACTGAGGTGA                GGACCTACTCC                 AGTCCCCAGTAGACGT GTCTG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 003-138953648
Forward Primer: 5'-CTTGGTGTCTGAGTGGCAAAACTG-3' T_m: 59.038
Reverse Primer: 5'-TATGGGCCTGTTTTACACGGGCCT-3' T_m: 60.908
Expected PCR Product Length: 281
Expected CelI Cleavage Product Sizes: 148, 133
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr3:138953773-138953815

Rank: 184	Homology Score: 8
	Genomic Region: Intergenic	Closest Gene: YIPF5
Match Type: R12R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-ACTGC TTCACCTGTCTCCTCC                CATTATAAATCT                TCAGGAGTCCTGTGGA GGTGG-3'
	3'-TGACG AAGTGGACAGAGGAGG                GTAATATTTAGA                AGTCCTCAGGACACCT CCACC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 005-143474515
Forward Primer: 5'-CCACTCTCCCTCAAATCAACTGC-3' T_m: 59.148
Reverse Primer: 5'-CTGCATTTCTGAGACAGACATGGC-3' T_m: 59.038
Expected PCR Product Length: 351
Expected CelI Cleavage Product Sizes: 202, 149
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr5:143474693-143474736

Rank: 185	Homology Score: 8
	Genomic Region: Intergenic	Closest Gene: RGS17
Match Type: R11R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CCTTG TGCAGCTGGCTCCCAT                GATGGCAGCCA                 AATGGAGGCTGGTGCA CTGTG-3'
	3'-GGAAC ACGTCGACCGAGGGTA                CTACCGTCGGT                 TTACCTCCGACCACGT GACAC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 006-153846994
Forward Primer: 5'-GCAAGCTGTCTCATGCTCACTG-3' T_m: 59.258
Reverse Primer: 5'-CAGGAAGGAACTTGTCAGATTCCC-3' T_m: 59.038
Expected PCR Product Length: 366
Expected CelI Cleavage Product Sizes: 229, 137
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr6:153847200-153847242

Rank: 186	Homology Score: 8
	Genomic Region: Intergenic	Closest Gene: BOD1
Match Type: R26R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CCTAC TTCACAGGACTGCTGT         TAGGATTCAACAAGGCAATGCCTGGG         TAAGCAGTAAGGTGCA GATAG-3'
	3'-GGATG AAGTGTCCTGACGACA         ATCCTAAGTTGTTCCGTTACGGACCC         ATTCGTCATTCCACGT CTATC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 005-173059880
Forward Primer: 5'-CGCTCAAGATTCGTGGACTTAGG-3' T_m: 59.148
Reverse Primer: 5'-CGGTGAACAGATTCCAGAGTGTG-3' T_m: 59.148
Expected PCR Product Length: 293
Expected CelI Cleavage Product Sizes: 139, 154
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr5:173059988-173060045

Rank: 187	Homology Score: 8
	Genomic Region: Intergenic	Closest Gene: MIR4424
Match Type: R26R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CCTCT TGAACCTGATGCCTTT         TCTGTTGAGGTTAATAGGGTGTAGGC         ACAGCAGTCAGGATGA ACTAG-3'
	3'-GGAGA ACTTGGACTACGGAAA         AGACAACTCCAATTATCCCACATCCG         TGTCGTCAGTCCTACT TGATC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 001-178611125
Forward Primer: 5'-CGGGGGTTATCTTGTCCACCA-3' T_m: 59.378
Reverse Primer: 5'-AAGGCGGGCTAGTTGTGTTGATC-3' T_m: 59.148
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 135, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr1:178611229-178611286

Rank: 188	Homology Score: 8
	Genomic Region: Intergenic	Closest Gene: CDH18
Match Type: R16R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GCAGT TTCCCCTGCCTCTTGT              ATCACTCCTTGGTCCT              ACAGGATACAAGTGTA GCATA-3'
	3'-CGTCA AAGGGGACGGAGAACA              TAGTGAGGAACCAGGA              TGTCCTATGTTCACAT CGTAT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 005-019467940
Forward Primer: 5'-GAGATCAGGCCACAGTCATATTCC-3' T_m: 59.038
Reverse Primer: 5'-CCTGGCGACAGTTGTCATCATC-3' T_m: 59.258
Expected PCR Product Length: 435
Expected CelI Cleavage Product Sizes: 234, 201
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr5:19468148-19468195

Rank: 189	Homology Score: 8
	Genomic Region: Intergenic	Closest Gene: USP22
Match Type: R10R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TACAA TGCATATGACAACTGT                 TACACAAGGG                 ACAGGAAGGAGGTGTA AATGG-3'
	3'-ATGTT ACGTATACTGTTGACA                 ATGTGTTCCC                 TGTCCTTCCTCCACAT TTACC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 017-020954067
Forward Primer: 5'-CGGGAAAAAGTACCAGATGGAGAC-3' T_m: 59.038
Reverse Primer: 5'-GACAGAGTGAGACCCTGTCTCAA-3' T_m: 59.148
Expected PCR Product Length: 346
Expected CelI Cleavage Product Sizes: 153, 193
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr17:20954197-20954238

Rank: 190	Homology Score: 8
	Genomic Region: Intergenic	Closest Gene: LOC284661
Match Type: R16R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-ATGCG TGCACCTGTGTTGTGT              GCATGTGCATATCCAT              ATAGGTGTGCGGTGCA CATGT-3'
	3'-TACGC ACGTGGACACAACACA              CGTACACGTATAGGTA              TATCCACACGCCACGT GTACA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 001-004413572
Forward Primer: 5'-TCCAGCCTCTCTCCTAGCTTTG-3' T_m: 59.258
Reverse Primer: 5'-GAGCTGGGAGGAGAAGTCTTTG-3' T_m: 59.258
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 135, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr1:4413681-4413728

Rank: 191	Homology Score: 8
	Genomic Region: Intergenic	Closest Gene: KIAA0226L
Match Type: R20R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-AAATA TGAATGTGACTCTTGT            TGGCAATGAAAGGTAGAAAC            GCATGAATCAAGTGCA GATGT-3'
	3'-TTTAT ACTTACACTGAGAACA            ACCGTTACTTTCCATCTTTG            CGTACTTAGTTCACGT CTACA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 013-046993177
Forward Primer: 5'-CATAACACCTGGCCTTCTGCATAG-3' T_m: 59.038
Reverse Primer: 5'-TTATGGGTCAGTGGTTGCTCTGC-3' T_m: 59.148
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 134, 136
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr13:46993283-46993334

Rank: 192	Homology Score: 8
	Genomic Region: Intergenic	Closest Gene: ALAS1
Match Type: R15R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TGAGC TGCAGTTGCCTCATGT              GAAATGAAGATTGTA               ACTGAAGCCAGGTGTA TCATT-3'
	3'-ACTCG ACGTCAACGGAGTACA              CTTTACTTCTAACAT               TGACTTCGGTCCACAT AGTAA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 003-052213069
Forward Primer: 5'-CATAGGGGTGAGCTGAGCTCAT-3' T_m: 59.258
Reverse Primer: 5'-GGCCTTTGGAAGGAGAATGGCT-3' T_m: 59.258
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 135, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr3:52213179-52213225

Rank: 193	Homology Score: 8
	Genomic Region: Intergenic	Closest Gene: MIR4790
Match Type: R19R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GGATT TGAACCTGGCTTCTCT            GCCACCTGCATTTTTGACC             ATGGGAGCCAGGAGCA GGGCG-3'
	3'-CCTAA ACTTGGACCGAAGAGA            CGGTGGACGTAAAAACTGG             TACCCTCGGTCCTCGT CCCGC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 003-005393709
Forward Primer: 5'-CATCTACAGAATAACCCCAGGAGG-3' T_m: 59.038
Reverse Primer: 5'-CCGTGTCACAGACAGTGTCTGAA-3' T_m: 59.148
Expected PCR Product Length: 310
Expected CelI Cleavage Product Sizes: 140, 170
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr3:5393822-5393872

Rank: 194	Homology Score: 8
	Genomic Region: Intergenic	Closest Gene: KCNQ2
Match Type: R11R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CAGGC TGGGCCTGGCTCCTGG                GGCCTGGACCT                 AGAGGAGGAAGGAGCA GGGCC-3'
	3'-GTCCG ACCCGGACCGAGGACC                CCGGACCTGGA                 TCTCCTCCTTCCTCGT CCCGG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 020-062024579
Forward Primer: 5'-GAAGGAGCAGGGCCATTGTG-3' T_m: 59.508
Reverse Primer: 5'-TTCCTCCTCTAGGTCCAGGC-3' T_m: 59.508
Expected PCR Product Length: 283
Expected CelI Cleavage Product Sizes: 139, 144
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr20:62024695-62024737

Rank: 195	Homology Score: 8
	Genomic Region: Intergenic	Closest Gene: KCNQ2
Match Type: R11R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CAGGC TGGGCCTGGCTCCTGG                GGCCTGGGCCC                 AGAGGAGGAAGGAGCA GGGCC-3'
	3'-GTCCG ACCCGGACCGAGGACC                CCGGACCCGGG                 TCTCCTCCTTCCTCGT CCCGG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 020-062024579
Forward Primer: 5'-GAAGGAGCAGGGCCATTGTG-3' T_m: 59.508
Reverse Primer: 5'-TTATTGCCCCAGGAGCCAGG-3' T_m: 59.508
Expected PCR Product Length: 335
Expected CelI Cleavage Product Sizes: 204, 131
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr20:62024760-62024802

Rank: 196	Homology Score: 8
	Genomic Region: Intergenic	Closest Gene: KCNQ2
Match Type: R11R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CAGGC TGGGCCTGGCTCCTGG                GGCCTGGACCT                 AGAGGAGGAAGGAGCA GGGCC-3'
	3'-GTCCG ACCCGGACCGAGGACC                CCGGACCTGGA                 TCTCCTCCTTCCTCGT CCCGG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 020-062024718
Forward Primer: 5'-ACCTAGAGGAGGAAGGAGCAG-3' T_m: 59.378
Reverse Primer: 5'-TGAGAGCCTGCAAGTCCCAC-3' T_m: 59.508
Expected PCR Product Length: 290
Expected CelI Cleavage Product Sizes: 130, 160
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr20:62024825-62024867

Rank: 197	Homology Score: 8
	Genomic Region: Intergenic	Closest Gene: KHDRBS2
Match Type: R18R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-AGCAC TGCAACTCATTCCTGG             TGCCCAGTGGCTCCTAGG             AAAGGGGTGAGGTGAA CAGGT-3'
	3'-TCGTG ACGTTGAGTAAGGACC             ACGGGTCACCGAGGATCC             TTTCCCCACTCCACTT GTCCA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 006-063449762
Forward Primer: 5'-TCTGAGGAGACCTTCAGAGGG-3' T_m: 59.378
Reverse Primer: 5'-ACCACCTCTTGAAGCAGCTCTC-3' T_m: 59.258
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 135, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr6:63449870-63449919

Rank: 198	Homology Score: 8
	Genomic Region: Intergenic	Closest Gene: OR7E156P
Match Type: R20R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-AAATA TGAATGTGACTCTTGT            TGGCAATGAAAGGTAGAAAC            GCATGAATCAAGTGCA GATGT-3'
	3'-TTTAT ACTTACACTGAGAACA            ACCGTTACTTTCCATCTTTG            CGTACTTAGTTCACGT CTACA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 013-064366457
Forward Primer: 5'-ACGTAACACCTGGCCTTCTGC-3' T_m: 59.378
Reverse Primer: 5'-TATGGGTCAGTGGTTGCTCTGC-3' T_m: 59.258
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 135, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr13:64366564-64366615

Rank: 199	Homology Score: 8
	Genomic Region: Intergenic	Closest Gene: LOC100505718
Match Type: R25R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GACAC TGGCCCTGATTCCTGG         TTTTCCTGCCCCTTAGTTCTGCAAA          ACAGGATCCAGGAGAA CCAGC-3'
	3'-CTGTG ACCGGGACTAAGGACC         AAAAGGACGGGGAATCAAGACGTTT          TGTCCTAGGTCCTCTT GGTCG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 008-070071702
Forward Primer: 5'-CCTGTTCTCGGAAGAAACACTGTC-3' T_m: 59.038
Reverse Primer: 5'-GCTTTCTAAATGGGAGAGTGTCGC-3' T_m: 59.038
Expected PCR Product Length: 365
Expected CelI Cleavage Product Sizes: 231, 134
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr8:70071903-70071959

Rank: 200	Homology Score: 8
	Genomic Region: Intergenic	Closest Gene: LOC100505817
Match Type: R17R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TTAAA TGTACCTTACAGCTGT             ACTTATGTGTCAGTTTT              ACAGAATGCATGTGCA TGGTT-3'
	3'-AATTT ACATGGAATGTCGACA             TGAATACACAGTCAAAA              TGTCTTACGTACACGT ACCAA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 018-071098003
Forward Primer: 5'-TGTCTGTTCTATCCTTCAGCACCC-3' T_m: 59.038
Reverse Primer: 5'-GAGGGAAATATTGCATGGGAGGTG-3' T_m: 59.038
Expected PCR Product Length: 409
Expected CelI Cleavage Product Sizes: 130, 279
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr18:71098107-71098155

Plate format Oligo Order Form
Well Name Sequence 5' mods Internal Notes
Primer Plate #1
A1 011-005248069F GTGCCTATCAGAAACCCAAGAGTC S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
A2 011-005248069R CAATCTACTCCCAGGAGCAGG S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
B1 011-005255477F GTCAGTGCCTATCAGAAACCCAAG S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
B2 011-005255477R TCACTGGAGCAGGGAGGACA S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
C1 022-045287459F CAAAGGCTCTGCCTGCAGGA S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
C2 022-045287459R CAGGACTAAGGCCGCTCTTC S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
D1 003-058640715F CATATGAGGAAACTGAGAGGCGG S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
D2 003-058640715R CCTCGAGGAGGGCTATTCTAGA S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
E1 005-059619945F CCAAGACTTAGCTTAGCACCCAAC S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
E2 005-059619945R CATGCCTGGAGTTAGCCTCTCT S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
F1 022-024555541F CACAGCCATTCTGTCTACTCCC S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
F2 022-024555541R TGGCAGGTGGCCAGTGTCTT S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
G1 007-002962408F ACAGCGTTTGTCTCCTGGCC S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
G2 007-002962408R GCCCCTAAGCCTTGCATTCAG S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
H1 007-003795640F CCTGACTCCTTTCACATGGCATAG S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
H2 007-003795640R TGTGTCACACGTGCTATGCCATG S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
A3 007-003795876F GAGTCCTTTCACATGGCATAGCAC S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
A4 007-003795876R ACATGTCTCACACGCACAATGCC S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
B3 006-101676033F CCAGGTAACATACTTGCTGTCTGC S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
B4 006-101676033R GTCACTACTCAGTACACTTGGACC S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
C3 006-110284189F GCAATGTTGCTCCAAGAGGCC S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
C4 006-110284189R GAACTTATTCCTCCCACCTAGCTG S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
D3 002-235805535F ACCTCCACCAGACCACGAATG S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
D4 002-235805535R GACCCTTAGCAGGTCACACTC S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
E3 020-045428486F GTCTCACTGAGACCTAGGCG S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
E4 020-045428486R TGGGCCTGGGGAACCAACAA S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
F3 009-129960176F CTTCCTTGGAGTGTCCAATGGG S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
F4 009-129960176R CATCAGGGCTTTTGGCCTCAAAC S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
G3 No PrimersF No Primers S2_S5_NN_Ranking-Homology_5T	No Primers
G4 No PrimersR No Primers S2_S5_NN_Ranking-Homology_5T	No Primers
H3 005-172280139F TGGTTTACCACGTGCTGTTGTTGC S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
H4 005-172280139R AAGATGCCTCTGGGTGGCCT S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
A5 004-186747672F CTGTGAGCTACTGAGGATATGCG S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
A6 004-186747672R GGGCAGATGCTTAAATCACTCCCA S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
B5 007-030006854F CCAAGAAGGTTATGCCCTGAATGC S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
B6 007-030006854R TAGTGGGTGTCCTTGGCTCTG S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
C5 003-052584959F GGCTAGGAATCTTAAGCACCCC S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
C6 003-052584959R GAGGGGATAGCAAGGCTGTG S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
D5 003-054731501F CCTACAGTTTCCAAGGACAAGCAG S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
D6 003-054731501R CCCAGCAGTAGCTTTAGCATCC S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
E5 016-071012605F CTCCTAGCTTCCTGATTCTTTGCC S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
E6 016-071012605R CCCACCCTGGTACAGCAAATG S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
F5 014-077327606F GCCTGGTATAGAACAAGCACCTG S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
F6 014-077327606R CCTCCTGTGATTGCCAGAGC S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
G5 012-081520008F GGCAGCAACTCTCCAGTGAGTA S2_S5_NN_Ranking-Homology_5T	Warning! Primer pair was generated using suboptimal parameters. 
G6 012-081520008R CCTGTATTTCCCTATGTATATGTGCTCAGTC S2_S5_NN_Ranking-Homology_5T	Warning! Primer pair was generated using suboptimal parameters. 
H5 014-100782711F TGAAGGGACAGAGCCAGGGT S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
H6 014-100782711R ACACCAGCCTGGGCAACAGA S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
A7 008-011765812F ATCCCCAGTAATGTACCTGCTGTC S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
A8 008-011765812R CTCAGTACACTTGCACCCGG S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
B7 004-190659833F CTGCCTCCAATCCCTCAGAAAATG S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
B8 004-190659833R CTGGTATTGGGTCCAGACCAC S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
C7 dom-000216374F TCTCCTTTCTGTACCTCAGAGACC S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
C8 dom-000216374R CCCACCCTGGTACAGCAAATG S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
D7 002-241334168F CATGCACCCCTGGCTTGTGT S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
D8 002-241334168R AGCTTTGCACCTGGAACCTGTGA S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
E7 018-055555293F TCTGGGAAGAAGCTGCCAGG S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
E8 018-055555293R GGCTATGCTACCTGCTGTAAAACC S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
F7 018-072086920F GCTGTCACCAGGAAAAACATAAGAGCC S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
F8 018-072086920R AAGCTGCTGAAATGGGCAGAGG S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
G7 019-009837811F AATCCCAGGTAGCGTACGTGC S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
G8 019-009837811R TCAGTACACATGGACCTGGCTTC S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
H7 005-137519020F GCGAGGATCAAAATGGCAATCCC S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
H8 005-137519020R GGCAGAGGACTAATGTTTCCTGG S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
A9 006-138657515F GTTCCCGTGCATCAGTCAGC S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
A10 006-138657515R GCTGATCAGTGGTTGGAAAGGC S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
B9 009-033466447F AATGGGCCTAGGACTGGGAG S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
B10 009-033466447R TCTCCCTCTCAGACCTCCAG S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
C9 011-006643499F TGGGAAGCCATAGTCCCCAG S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
C10 011-006643499R ATCAATGAGTTCCCCCGTGTGG S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
D9 002-101527279F CATGCCCAGCCTAATGTCTTCTTC S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
D10 002-101527279R GCCAGAGGAGGTTATTTTCTTGCC S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
E9 010-103998000F CTTCAGGTTAGTCTAGGAGGCATC S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
E10 010-103998000R TGACAGACGGGCCACCCTAT S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
F9 011-105719867F CAGATTGCGCCACTGCATTCC S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
F10 011-105719867R TGTCTCCTTCAGAACTGAGCTGG S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
G9 004-110007835F GACTGCTGGGAATTGCTCTTTGAC S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
G10 004-110007835R TCCCTCAACTTCCACTGGGC S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
H9 001-114253666F AGGTGTTCTGGGAGGGGAGT S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
H10 001-114253666R GAGTAGATGGTGGTGGTGTGG S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
A11 011-126353104F GAGGTCATACCCCCTTTTTTGTCC S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
A12 011-126353104R GGGCCCAGTTCTGAATCAAACAGC S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
B11 002-130879063F AAGACGCCCACCGAGCCCA S2_S5_NN_Ranking-Homology_5T	Warning! Primer pair was generated using suboptimal parameters. 
B12 002-130879063R GCTAATGGGTGCAAAGTTTCTTTTAAGGAGCATA S2_S5_NN_Ranking-Homology_5T	Warning! Primer pair was generated using suboptimal parameters. 
C11 006-132465014F GTTGTGTTGTGGCTGCTCAGAGT S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
C12 006-132465014R CCTTCTATCCCCATCCTCTCTTTC S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
D11 009-134936820F GCTGGTAAGTGAAGTTGATGTGGC S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
D12 009-134936820R CCAGGAGCAGCTGCGATGAA S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
E11 005-136419378F GCTATGGGAGAGCCGAAAACTG S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
E12 005-136419378R TGGAGTGGGCAAGCAAGAGC S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
F11 005-016519335F CCTAGATCAGCGGCTTTCAAAGCA S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
F12 005-016519335R GGGCTGAAAGGAGCTTTGAGG S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
G11 004-170092193F GACCTAAAGGTTTCTCCACACATAGTGAAG S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
G12 004-170092193R GACTGTTCTGAGATCTTGGGTGTG S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
H11 005-177943719F AAGCTTTCTGCCTCCTCAGGCC S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
H12 005-177943719R CACCTCAGTGACAGGCGCTT S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
Primer Plate #2
A1 009-019336805F GTCCATTGATAATCACTCTAGCACAGGTAC S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
A2 009-019336805R GTGTGACTTCCTTCCTTCCCTC S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
B1 017-020300643F GGTGACAGAGCAAGACTCCATC S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
B2 017-020300643R CTCTGTGGGTTAGTGCCTAATCTG S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
C1 002-219751327F TGTGTCTTAACGAGCCGCCC S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
C2 002-219751327R CTGACAGCTGTCATAGCCACG S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
D1 001-227379954F GAGATCACTCTCACCACCCAAAAC S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
D2 001-227379954R GGAAACAGCAGAACGTTGTTGGTG S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
E1 001-230976433F GCGCCAAGGTCTTCTTGAGC S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
E2 001-230976433R GGAGAGTAGGAGACAGCACATG S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
F1 002-023872858F GTGGTCTTAGGCACCTCCTG S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
F2 002-023872858R CTCACTGTCCCCTTAACTTTCCTG S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
G1 021-027390640F CCTCCCTGCAGTATCTCATCTTTG S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
G2 021-027390640R GCAGGTCCATTCATTCCACCTC S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
H1 011-027668392F GGTGGGTCCATTCTGACTCTG S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
H2 011-027668392R TCATGATCCACCCACCTCGG S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
A3 003-030699112F CTGGCACAGAGTGAGCACTTAATG S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
A4 003-030699112R GGTCGTCATGAGGAGAACACCTT S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
B3 017-003965044F CGTAAGTCCTGACAACTCTACCTC S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
B4 017-003965044R GAAAGGCAGGACCCTTATCACTGT S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
C3 012-040234817F CCTTACAATTCACGGCTCTCAGAC S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
C4 012-040234817R CCACTGGAGTTGACAGATTTGGC S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
D3 008-041570215F GGGTGCAGAGAGATTTGCATGTG S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
D4 008-041570215R TGTCTCCTCCTGTCCCTGCT S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
E3 010-045378787F GGTGAGGCTCCATCCCACAA S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
E4 010-045378787R ACCCAGAGAGGGTGTGAGAG S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
F3 001-045422945F GCCCCCAAACACCACAAAGTC S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
F4 001-045422945R ATTACAGGCACATGCCACCACG S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
G3 019-046327426F GTGCTTCGTTCACTGGTGAAGATG S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
G4 019-046327426R GTCCAGCTGTGGAAAAGTGATCTC S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
H3 012-052789060F ATTCCTCCCACCTCCTGCCA S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
H4 012-052789060R TTCAGGGCCCAGAAGCCACA S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
A5 004-054438610F CCCAGGCCATTGCTTTTGATGC S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
A6 004-054438610R GGCTGCCTCTCATTTTGTCCAC S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
B5 020-059992627F TGGCCCTGTTTCTGGCTGGA S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
B6 020-059992627R AGTACACTGAAGTGCAGTCTCTGG S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
C5 020-060508972F CATGGTTCTGTGGGATGCTTGG S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
C6 020-060508972R ACCATGGGCCTGATCGGGTA S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
D5 013-067512369F GGCATCAACAGGGCTTCTTAAACG S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
D6 013-067512369R CAATGTCAGTCGTTTCTGCATGAAGATAGG S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
E5 016-068387900F GCGCCAGTGGTCCTTGGATT S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
E6 016-068387900R CTGGGAATACAGGTGTGCACTAC S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
F5 015-072957610F TACTCACCTCTTGCCTTCAAGTGG S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
F6 015-072957610R CTTTTCTTGGCCCATGCCAGG S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
G5 013-073489472F GCGCTCTGCTTCTAAGACACATTG S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
G6 013-073489472R CACATGGAAGAGATGATACATGCCATTACC S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
H5 00X-073723246F GTACAGTGGGGAGTCTGGAG S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
H6 00X-073723246R CAGAAGACCAGAAGTAGGCCTAAC S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
A7 015-074364061F TTGGCCCATGCCAGGACTCA S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
A8 015-074364061R GCCCCTACAAGAATACTCACCTC S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
B7 018-074790610F GAGCTGTGGACTGAAGAGCAAC S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
B8 018-074790610R CACAGTCTTGGGAGGGTGCT S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
C7 015-075561464F TACTCACCTCTTGCCTTCAAGTGG S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
C8 015-075561464R TTCTTGGCCCATGCCAGGAC S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
D7 015-075585740F TACTCACCTCTTGCCTTCAAGTGG S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
D8 015-075585740R TTCTTGGCCCATGCCAGGAC S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
E7 005-075922611F AGGGTAGCCCGGGAAGACTTT S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
E8 005-075922611R TAAAGCTTCCCCAAAGGCTTCCC S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
F7 014-077327823F CCAACAGCTGGTAAGGATGTCAAG S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
F8 014-077327823R CTGTGGAAACACCAAAGCAGGG S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
G7 015-079604259F TTGGAAAGGAGGGATCAGTGCAG S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
G8 015-079604259R GTGCCTATCAGCATGCGTCC S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
H7 007-008187341F CACACACACACACACACACAAGC S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
H8 007-008187341R ACACAGGATGGGAACCAGTCCTA S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
A9 002-008374518F TCCCGGAAACATGTCAGCCC S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
A10 002-008374518R CATCCTACATCCCCTGAACATGG S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
B9 006-083995632F CAGTTGTTCGGATACGGCTTATCTTCATG S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
B10 006-083995632R GGGTAAGAAAACAGAGGCACAGAG S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
C9 007-008646994F GGATCGTGTCACTCGATACAGTAC S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
C10 007-008646994R GGGTCTAGCAACATATTCCCACAG S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
D9 017-009048384F AGCTGAGGCAGGCGCATGAA S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
D10 017-009048384R CCCGGTGGCGTTGTTTTATGAC S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
E9 017-009569404F GGGAATGAAAAGGACAGCAGGAC S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
E10 017-009569404R CGGCCCTGCGCGTTATTTCT S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
F9 017-009569404F GGGAATGAAAAGGACAGCAGGAC S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
F10 017-009569404R CGGCCCTGCGCGTTATTTCT S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
G9 008-096438369F GACCAAGCCCCAGAATGGTTAATC S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
G10 008-096438369R CAGCTCCCCCTAAACTACCAG S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
H9 001-104689409F GTTGAGGGAGTCATCCTGAGC S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
H10 001-104689409R GGTTTCACTGTGTTAGCCAGGATG S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
A11 006-106935880F TGGTGCATTGACTTTGGTTCTGGG S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
A12 006-106935880R TCTGACCCAGACCTGACAGATAG S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
B11 008-107911219F GGCTTCACTTTAGGTCTCTCTTCC S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
B12 008-107911219R CCATAGCAAGCCATTTCAGCCTTC S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
C11 019-011375833F GAGAGTGAGACCCTGTCTCAAAAC S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
C12 019-011375833R TAGGCTACATTAGGGCGAGTCC S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
D11 001-118086389F CTGTTCATTTTCTTAAGGTGGAGACTTACCTG S2_S5_NN_Ranking-Homology_5T	Warning! Primer pair was generated using suboptimal parameters. 
D12 001-118086389R GCACTCTGGAAACATTCTCTTTCAATGTGTC S2_S5_NN_Ranking-Homology_5T	Warning! Primer pair was generated using suboptimal parameters. 
E11 004-118702135F GCAGGGACACTGGTGTTATGTCT S2_S5_NN_Ranking-Homology_5T	Warning! Primer pair was generated using suboptimal parameters. 
E12 004-118702135R GATAGTAACTTATTTAGAATTGAGGTTGTGTGATGGAG S2_S5_NN_Ranking-Homology_5T	Warning! Primer pair was generated using suboptimal parameters. 
F11 008-122428182F ACAAGTTCTGCCAACTACACCAGG S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
F12 008-122428182R AGAGCCATTCCCAAGTGGGGT S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
G11 002-131974522F GCTAATGGGTGCAAAGTTTCTTTTAAGGAGCATA S2_S5_NN_Ranking-Homology_5T	Warning! Primer pair was generated using suboptimal parameters. 
G12 002-131974522R AAGACGCCCACCGAGCCCA S2_S5_NN_Ranking-Homology_5T	Warning! Primer pair was generated using suboptimal parameters. 
H11 004-132113714F GCTGTTCTGAGGTTGTTCCTGTG S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
H12 004-132113714R CCAGACTGAAGCATAGTCCCATGA S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
Primer Plate #3
A1 005-141074133F TCTGCTTGGGCCTTAGAGGG S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
A2 005-141074133R ATTCAGCATCCCTGCCCCAGA S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
B1 008-142885698F GTGAACTACCTCTCTGGCACTG S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
B2 008-142885698R CTCACCCAAAACTGGAAGCAACC S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
C1 00X-148285072F GGAGCAAAAAGGACTATGTCACTGAGTCAA S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
C2 00X-148285072R CCACGTAAGTCCTACAGGTGACTA S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
D1 004-150284205F GAGGAAGTCCCCAGTGAGAAAATG S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
D2 004-150284205R TATTCCTGGACCCACCCTACTTC S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
E1 007-156274056F CAGGCGAGGTCTTACCCGAT S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
E2 007-156274056R GTCCACGTGTTCCCATTTTTGGC S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
F1 002-170227380F GGCCAGAAGACCAAGATGATTCC S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
F2 002-170227380R GCCAAAAGCCAAATGTAGACTTTGTGCTG S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
G1 005-174033940F CAGGAAGTCTTCCTTGACTGCAC S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
G2 005-174033940R GGCAGGCATTTTCCAGATGAGG S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
H1 004-182299969F GACAAGGAAACTGAGGCACAGAAG S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
H2 004-182299969R ACACTGATTCCTGACCTCAGCG S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
A3 002-000185249F CCACTGACCATAGACTGGTTCTG S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
A4 002-000185249R CCTGAACATTCCTGCAACCTTCAC S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
B3 001-195936317F GGACCATCAGCAGATAGTACGAAG S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
B4 001-195936317R GTTGGGGTCAGTTTCCTAGGC S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
C3 011-002239013F AGCTGTCCAGCCTGCAGCAT S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
C4 011-002239013R TCATATTCCTGGGTGGTCACTCC S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
D3 001-230749531F CAAAGATGCAAAGGCTGATTTAGAGCACC S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
D4 001-230749531R CCCCAAATTTAGCAATACTGTCTGCTGGA S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
E3 004-024170953F CTGGATGGGATCCCAGAACAGA S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
E4 004-024170953R GAGCCAACATGCTTGGCTGAG S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
F3 002-241889867F TCAGAGCCTGAAGCAGGAGC S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
F4 002-241889867R GGGCAAGTGGATGGGTGGAT S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
G3 014-024242484F CAAGTCTTCCTCAGCCATCAGACA S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
G4 014-024242484R CCAGACACATCAACAGAGTGGAAG S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
H3 007-002427539F TTGGCCAGGCTGGTCTCGAA S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
H4 007-002427539R CTTTGATGAACCCACAGGGCG S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
A5 011-031965225F CCAGCCTGAGACAACCATCTTTAC S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
A6 011-031965225R GAAGGGCCATAACTTGGGCATTAG S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
B5 014-032435668F GCAGTAAGCTTGTTTCCTTGGCC S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
B6 014-032435668R AACATGGGCTTCTTGCAAGGAGAG S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
C5 00X-034076224F AGTATTGCCCTTTCTGCTAGGGAC S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
C6 00X-034076224R CACTGCACACTTCTGTGGCTC S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
D5 001-034895940F TTACCTGGGAGGTGGAAACATGG S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
D6 001-034895940R CCTTCCTTGCTTTGTTGGTGGATG S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
E5 021-035539617F GGCCTTCCTCACTATCTTACAGTC S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
E6 021-035539617R GCAAAACCGTGTCTGCCCAAATC S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
F5 001-036588039F GCACATGTGTGTCCACATATGGC S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
F6 001-036588039R GGGCAGAGTTTAAGGAGAAAGGTG S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
G5 010-038882221F GCAATCATTGAATGGACTGGAAAGGAATCATC S2_S5_NN_Ranking-Homology_5T	Warning! Primer pair was generated using suboptimal parameters. 
G6 010-038882221R CCATGCAATGATGATTCCATTCGATTCCATTC S2_S5_NN_Ranking-Homology_5T	Warning! Primer pair was generated using suboptimal parameters. 
H5 005-039782571F GTTAAAGACGTGAGAGAGAAAACCAACAGC S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
H6 005-039782571R CTGAGGAACTATTCTGAGGGGC S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
A7 020-040489952F GTGCCACCCACACAGCATTC S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
A8 020-040489952R GCCAGCAATGTAGAGGGACAATG S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
B7 004-043460367F GCTAAGAGCCTCTTGGGCTTTTG S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
B8 004-043460367R AGCAGAGGCAGAAAGGACCAG S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
C7 020-044380819F GCTTCCAGGAATTACTCCTGTGC S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
C8 020-044380819R GGCAATTACTCAAGTGCCCAGAAG S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
D7 004-044831243F GCCACTATGTTTGCTTCCCAACTC S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
D8 004-044831243R CTCTACCTGGCAGCCTTCATTG S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
E7 010-004489187F GGTAGCCTGCACTCATGATTCTG S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
E8 010-004489187R CTCACAGCACCTATGACGGTTC S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
F7 017-045024202F CTGGGTGACGAAATGAGACTCTG S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
F8 017-045024202R TTCATGTCAGGGTCCCCTGG S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
G7 013-046233753F CTCCCTATGTTGTCCAGGCTAGA S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
G8 013-046233753R GCTGGGTTTGGTAGTTCTAACTCC S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
H7 001-054689628F GTGAAGGCAACTGCCAGGCT S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
H8 001-054689628R GGCTTTTGCTGAGTACAGACACC S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
A9 016-059193695F GTAGCCTCTCCCAATACTTAACGG S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
A10 016-059193695R CCTGTGTTGTTTCTGTCCAAGGC S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
B9 015-060382268F GAGAACTCAGTGATGTGGGAGATG S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
B10 015-060382268R CCTCCCCAAATACGCCACTTTAG S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
C9 001-061258016F CCAGTAGTTCCATCAAACTAGGGC S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
C10 001-061258016R GTCCTGTTCTCTGCTGCAGTTG S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
D9 011-065255104F TGGGGGAGAAAGGGAAAGGC S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
D10 011-065255104R AGGCGGAGGCAGAGTAGGTA S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
E9 009-066282880F ACTACAGGTGCACACCACCATG S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
E10 009-066282880R GCCTTCCCCAAGTTTGCATTTTCG S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
F9 011-067617612F GCTCTCCCCCTTTCGGATAG S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
F10 011-067617612R GTACACTTGGACCGGGCTTC S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
G9 017-068268613F AGGAAGGGATTCTCCTAATAAATTGGCAGATG S2_S5_NN_Ranking-Homology_5T	Warning! Primer pair was generated using suboptimal parameters. 
G10 017-068268613R CTATGGTCCATGTCTAACGTTAATACTACATGC S2_S5_NN_Ranking-Homology_5T	Warning! Primer pair was generated using suboptimal parameters. 
H9 005-006875444F CTGTGGCAGTTACTCAGATTTCCC S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
H10 005-006875444R GCAGAAACCTGACAAACAGGACC S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
A11 002-071249464F TAGTGTACACTTGGACCGGGC S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
A12 002-071249464R ATCCCAAGTAAGGTACCTGCTGTC S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
B11 015-076078165F TACTCACCTCTTGCCTTCAAGTGG S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
B12 015-076078165R CTTTTCTTGGCCCTTGCCAGGA S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
C11 018-076246512F AGCCTCCTACTGATGGGTGG S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
C12 018-076246512R GCTGATGAAGAAGAGGATGGGG S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
D11 018-076246512F AGCCTCCTACTGATGGGTGG S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
D12 018-076246512R GCTGATGAAGAAGAGGATGGGG S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
E11 005-083704636F GGAACGGATTAAAGGGAAGCCAG S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
E12 005-083704636R GGTCTGTTTCTGTAACATCAGAGGCAAAG S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
F11 00X-088338909F CCCCCCCAGAAAAGTGGTTTATG S2_S5_NN_Ranking-Homology_5T	Warning! Primer pair was generated using suboptimal parameters. 
F12 00X-088338909R CAGATTTGGCTCACAGTAGCATACTTTACTC S2_S5_NN_Ranking-Homology_5T	Warning! Primer pair was generated using suboptimal parameters. 
G11 009-096644130F CCCCCTTACATAGTGTGGAAGC S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
G12 009-096644130R TCGGAGTTTCTTCCTTCTGGTGG S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
H11 012-104495588F CATTCCATGTGAGAGAGAGGGAG S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
H12 012-104495588R TCTGACTGCAGAAGGAGCTCCA S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
Primer Plate #4
A1 ap1-000102177F AGATGCTCTCCTCTCCTGGC S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
A2 ap1-000102177R TGAGAGCCAGGTCCCTCTTG S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
B1 003-010453381F TGTTGCAGACACTGGAACAGAGC S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
B2 003-010453381R CGTGAGAGTGGACTTGCAAGTCTG S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
C1 001-109847447F GCAGGCTTGCCAAACTAAAAGCTC S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
C2 001-109847447R TCAGAGAGCCAACAGAGCCC S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
D1 001-011023947F TTTAATCCCCCCTCCCCTCC S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
D2 001-011023947R AAAGCGTCCACTGTGGGCCCAT S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
E1 005-012711008F GGTCATTCTTGCTCAGTTTGCCC S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
E2 005-012711008R GGGAGGGAGAGCATCAGGAA S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
F1 010-131523914F CTCGGAGGTCTGTCCATGCT S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
F2 010-131523914R GAGCTAGAAGGGGCTGGTTG S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
G1 016-015162374F GCAGGCTGTAAGATTCCACGAC S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
G2 016-015162374R GTACAAGTTGGTCAGCTTGCCTC S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
H1 019-017971876F CTCAATTAATCCTCCCTGGAGACC S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
H2 019-017971876R ATTCGCATGCGGTAGAGGGG S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
A3 003-183756342F AGGGAGATGCTGTCCCACTG S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
A4 003-183756342R TCCATTCTGAGCCCCCTGTC S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
B3 003-183824037F CCTGCTGCTCCACTGCAACA S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
B4 003-183824037R TCCATTCTGAGCCCCCTGTC S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
C3 001-019065509F AGAGTCCCATGTCCTGGACTG S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
C4 001-019065509R TGCTCAGTGGCCTCCACAAATC S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
D3 001-213140398F ATTCTAAGAGTGGCGTGTGGAGG S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
D4 001-213140398R GTTGTGGGTGATTCCAATGGCATG S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
E3 016-021809420F GGCTGTAAGATTCCACGACAACG S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
E4 016-021809420R GTACAAGTTGGTCAGCTTGCCTC S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
F3 016-022444397F GGCTGTAAGATTCCACGACAACG S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
F4 016-022444397R GTACAAGTTGGTCAGCTTGCCTC S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
G3 002-242219169F CACTTCTGGAGACGTACTTCAGG S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
G4 002-242219169R CTGCTGCCAAGGAAGTGGTG S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
H3 009-027247215F CAAGCACAGGCAGATGCTTTAGG S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
H4 009-027247215R CATCACGACTGTTGCCTGTTCTTG S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
A5 016-029109731F CTTGTTTTTGTAAGCAGTGACATCCCATTTAGG S2_S5_NN_Ranking-Homology_5T	Warning! Primer pair was generated using suboptimal parameters. 
A6 016-029109731R CCACGACAACACGAAGTTCATACG S2_S5_NN_Ranking-Homology_5T	Warning! Primer pair was generated using suboptimal parameters. 
B5 022-036626609F CTGACTCCAGGCTCTGACAC S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
B6 022-036626609R GCTGGGGCCTTTTTTCTCATCTG S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
C5 006-036744134F TCTCTGCTCCTTCTCGCCCA S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
C6 006-036744134R TGGGGGGTGGTATTTGGAGC S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
D5 020-042646910F GCTGGGCTCCTAATCAGAGCA S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
D6 020-042646910R AGATGGCTTCTCAGCAATGCCG S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
E5 017-043487041F AGATGCTCTCCTCTCCTGGC S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
E6 017-043487041R TGAGAGCCAGGTCCCTCTTG S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
F5 007-044570049F GGAGATGGATGGTGGTGATAGTTG S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
F6 007-044570049R TTAGGCAGTACTGGCCTGGG S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
G5 002-061428882F GGTTAAAGCTGTCCGAGTAGCTAG S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
G6 002-061428882R GCTGGTTCCTGGTAACCTGG S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
H5 020-062498891F CCTGGGTGACAGAGTAAGACTCG S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
H6 020-062498891R CCAATATGGTGAAACCCCGTCTC S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
A7 008-065585407F CATCAATGAGTGAGCCCCTTTGG S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
A8 008-065585407R GGAGAAACTCCACAAGACTAGAGC S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
B7 005-078702362F GAAAGGATCCTGGATGAGGGG S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
B8 005-078702362R GTAGGAGTTTCCTGCCTCTGTC S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
C7 016-084515616F AGCTGAGCTTCGGCGTGGAT S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
C8 016-084515616R ATGGTGTCAGCGCCAGACCT S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
D7 016-088575852F CCATTAGGCACACAGGGAGG S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
D8 016-088575852R CTGCCTCCTGAACCCACTCT S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
E7 002-009410094F CCTCGGTAACTTGAGAGGAACCTA S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
E8 002-009410094R AAAACTCCACTCCAGCTCAGAGCG S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
F7 002-101420981F GAGATTTGGGTGGGAACACAGAG S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
F8 002-101420981R AAACACCAGAGGTTTGTGTGGGC S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
G7 005-010678604F CCTGGGGGTGGTTCTGTTCCAT S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
G8 005-010678604R GAGCACCAGAGAGAGCACCA S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
H7 009-110904790F AAGCGGGTGGGGTAACAGTG S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
H8 009-110904790R CAGTGGAGGCTATTGTGTTTGTGC S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
A9 008-011624011F CCCAATCCAGGAGGCAGACA S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
A10 008-011624011R CCACCACACCCTGCTAATTCTTG S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
B9 008-121929055F ATTCCCCACATCACTGCACAAGC S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
B10 008-121929055R GCAGATACTGTGTGGGAAGCATTG S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
C9 009-124993691F GTGAAGCATGTGCTCCTAGAGTG S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
C10 009-124993691R GAAGAACTTGTCCAGGGTTACCCA S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
D9 011-012966658F TGAGGGATAGAGCAGAGGATGG S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
D10 011-012966658R GGGGGAATGATGTCACTGGAAC S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
E9 002-131671419F ACCTCCCTTCCTAGGGGTAC S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
E10 002-131671419R ACAGCGTTTCAGAATCATCGCGG S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
F9 008-001326103F TTCACGCACCCCTGTGCAGA S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
F10 008-001326103R TGTGCAGGACACACAGGTGAG S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
G9 003-138953648F CTTGGTGTCTGAGTGGCAAAACTG S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
G10 003-138953648R TATGGGCCTGTTTTACACGGGCCT S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
H9 005-143474515F CCACTCTCCCTCAAATCAACTGC S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
H10 005-143474515R CTGCATTTCTGAGACAGACATGGC S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
A11 006-153846994F GCAAGCTGTCTCATGCTCACTG S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
A12 006-153846994R CAGGAAGGAACTTGTCAGATTCCC S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
B11 005-173059880F CGCTCAAGATTCGTGGACTTAGG S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
B12 005-173059880R CGGTGAACAGATTCCAGAGTGTG S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
C11 001-178611125F CGGGGGTTATCTTGTCCACCA S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
C12 001-178611125R AAGGCGGGCTAGTTGTGTTGATC S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
D11 005-019467940F GAGATCAGGCCACAGTCATATTCC S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
D12 005-019467940R CCTGGCGACAGTTGTCATCATC S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
E11 017-020954067F CGGGAAAAAGTACCAGATGGAGAC S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
E12 017-020954067R GACAGAGTGAGACCCTGTCTCAA S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
F11 001-004413572F TCCAGCCTCTCTCCTAGCTTTG S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
F12 001-004413572R GAGCTGGGAGGAGAAGTCTTTG S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
G11 013-046993177F CATAACACCTGGCCTTCTGCATAG S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
G12 013-046993177R TTATGGGTCAGTGGTTGCTCTGC S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
H11 003-052213069F CATAGGGGTGAGCTGAGCTCAT S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
H12 003-052213069R GGCCTTTGGAAGGAGAATGGCT S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
Primer Plate #5
A1 003-005393709F CATCTACAGAATAACCCCAGGAGG S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
A2 003-005393709R CCGTGTCACAGACAGTGTCTGAA S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
B1 020-062024579F GAAGGAGCAGGGCCATTGTG S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
B2 020-062024579R TTCCTCCTCTAGGTCCAGGC S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
C1 020-062024579F GAAGGAGCAGGGCCATTGTG S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
C2 020-062024579R TTATTGCCCCAGGAGCCAGG S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
D1 020-062024718F ACCTAGAGGAGGAAGGAGCAG S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
D2 020-062024718R TGAGAGCCTGCAAGTCCCAC S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
E1 006-063449762F TCTGAGGAGACCTTCAGAGGG S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
E2 006-063449762R ACCACCTCTTGAAGCAGCTCTC S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
F1 013-064366457F ACGTAACACCTGGCCTTCTGC S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
F2 013-064366457R TATGGGTCAGTGGTTGCTCTGC S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
G1 008-070071702F CCTGTTCTCGGAAGAAACACTGTC S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
G2 008-070071702R GCTTTCTAAATGGGAGAGTGTCGC S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
H1 018-071098003F TGTCTGTTCTATCCTTCAGCACCC S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
H2 018-071098003R GAGGGAAATATTGCATGGGAGGTG S2_S5_NN_Ranking-Homology_5T	Primer pair generated using optimal parameters.
3481 total sites were located
Mismatch Types	Genomic Regions
0 and 2: 1 2 and 2: 1 3 and 3: 3 2 and 5: 8 3 and 4: 18 3 and 5: 112 4 and 4: 63 4 and 5: 813 5 and 5: 2462	Exons: 143 Promoters: 28 Introns: 1383 Intergenic: 1927