Top Cleavage Sites for S2_S5_NN

PROGNOS Summary for S2_S5_NN_Ranking-Homology

The hg19 genome was searched for the TALEN pair targeting:
5'- TCACCTTGCCCCACAGGGCAGT NN..NN ACAGGAGTCAGGTGCA -3'

Up to 5 mismatches were allowed in each nuclease half-site.

Allowed spacing distances were: 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30

Homodimers and heterodimers of the nucleases were included in the output

7329 total sites were located
Mismatch Types Genomic Regions
  0 and 2: 1
  2 and 2: 1
  3 and 3: 5
  2 and 5: 18
  3 and 4: 26
  3 and 5: 182
  4 and 4: 102
  4 and 5: 1568
  5 and 5: 5426
  Exons: 358
  Promoters: 77
  Introns: 3048
  Intergenic: 3846


The top 200 sites are ranked below according to a weighted sequence homology.
For the full output, click here.

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Primer pairs were designed using the 400 bp of sequence on either side of the cleavage site to melt close to 60 °C

For easy visualization of cleavage on gels:
The smallest fragment will be longer than 130 bp, which is also the minimum separation distance between uncleaved and cleaved products.
The two cleavage products will be separated by a minimum size of 0 bp
To optimize assay conditions, the amplicons have a minimum length of 260 bp, a maximum length of 450 bp, and an optimal length of 270 bp.
Rank: 1	Homology Score: 52
	Genomic Region: Exon	Closest Gene: HBB	Caution! This site scores very closely to the intended nuclease target. The potential for cleavage at this site is of great concern.
Match Type: L16R
	           Left Nuclease
	         TCACCTTGCCCCACAGGGCAGT
	5'-CACGT TCACCTTGCCCCACAGGGCAGT        AACGGCAGACTTCTCC              TCAGGAGTCAGATGCA CCATG-3'
	3'-GTGCA AGTGGAACGGGGTGTCCCGTCA        TTGCCGTCTGAAGAGG              AGTCCTCAGTCTACGT GGTAC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 011-005248069
Forward Primer: 5'-GTGCCTATCAGAAACCCAAGAGTC-3' T_m: 59.038
Reverse Primer: 5'-CAATCTACTCCCAGGAGCAGG-3' T_m: 59.378
Expected PCR Product Length: 308
Expected CelI Cleavage Product Sizes: 154, 154
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr11:5248194-5248247

Rank: 2	Homology Score: 32
	Genomic Region: Exon	Closest Gene: HBD	Caution! This site scores very closely to the intended nuclease target. The potential for cleavage at this site is of great concern.
Match Type: L16R
	           Left Nuclease
	         TCACCTTGCCCCACAGGGCAGT
	5'-CACGT TCACTTTGCCCCACAGGGCATT        GACAGCAGTCTTCTCC              TCAGGAGTCAGATGCA CCATG-3'
	3'-GTGCA AGTGAAACGGGGTGTCCCGTAA        CTGTCGTCAGAAGAGG              AGTCCTCAGTCTACGT GGTAC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 011-005255477
Forward Primer: 5'-GTCAGTGCCTATCAGAAACCCAAG-3' T_m: 59.038
Reverse Primer: 5'-TCACTGGAGCAGGGAGGACA-3' T_m: 59.508
Expected PCR Product Length: 296
Expected CelI Cleavage Product Sizes: 158, 138
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr11:5255606-5255659

Rank: 3	Homology Score: 18
	Genomic Region: Intron	Closest Gene: PHF21B
Match Type: R12R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CATTC TCCACCTGTCTCCTGC                CTCTCAACTGGG                TCTGGAGTCAGGTGGA AAAAT-3'
	3'-GTAAG AGGTGGACAGAGGACG                GAGAGTTGACCC                AGACCTCAGTCCACCT TTTTA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 022-045287459
Forward Primer: 5'-CAAAGGCTCTGCCTGCAGGA-3' T_m: 59.508
Reverse Primer: 5'-CAGGACTAAGGCCGCTCTTC-3' T_m: 59.508
Expected PCR Product Length: 269
Expected CelI Cleavage Product Sizes: 136, 133
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr22:45287571-45287614

Rank: 4	Homology Score: 18
	Genomic Region: Intron	Closest Gene: FAM3D
Match Type: R17R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CCCTG TGCCCCTGACTCCTTA             CAGCTGTTCTAGAATGT              AAATGAGGCAGGTGCA TTTCG-3'
	3'-GGGAC ACGGGGACTGAGGAAT             GTCGACAAGATCTTACA              TTTACTCCGTCCACGT AAAGC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 003-058640715
Forward Primer: 5'-CATATGAGGAAACTGAGAGGCGG-3' T_m: 59.148
Reverse Primer: 5'-CCTCGAGGAGGGCTATTCTAGA-3' T_m: 59.258
Expected PCR Product Length: 283
Expected CelI Cleavage Product Sizes: 130, 153
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr3:58640819-58640867

Rank: 5	Homology Score: 18
	Genomic Region: Intron	Closest Gene: PDE4D
Match Type: R29R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CACAC TGCACCTAACATCTGT       CCTTATAATAAACTAGGAGCATCTCTGTT        ACAGGAATCATGTTCA GTAGT-3'
	3'-GTGTG ACGTGGATTGTAGACA       GGAATATTATTTGATCCTCGTAGAGACAA        TGTCCTTAGTACAAGT CATCA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 005-059619945
Forward Primer: 5'-CCAAGACTTAGCTTAGCACCCAAC-3' T_m: 59.038
Reverse Primer: 5'-CATGCCTGGAGTTAGCCTCTCT-3' T_m: 59.258
Expected PCR Product Length: 384
Expected CelI Cleavage Product Sizes: 219, 165
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr5:59620132-59620192

Rank: 6	Homology Score: 18
	Genomic Region: Intergenic	Closest Gene: FLJ11235
Match Type: R29R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TAGGC TGCATCTTGCTCCTGT       ACATGGATGACCTTCTGCTGGGACACTCC        ACGGCAGTCAGGTGCG CAAAA-3'
	3'-ATCCG ACGTAGAACGAGGACA       TGTACCTACTGGAAGACGACCCTGTGAGG        TGCCGTCAGTCCACGC GTTTT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 005-111863094
Forward Primer: 5'-AGTACACTTGGACCCAGCTTACC-3' T_m: 59.148
Reverse Primer: 5'-TGAATCCCAGGTAGCGTACCTGC-3' T_m: 61.088
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 136, 134
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr5:111863198-111863258

Rank: 7	Homology Score: 18
	Genomic Region: Intergenic	Closest Gene: PKIB
Match Type: R29R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TTTAG CACACCTGACTGCTGT       GGAGTGTCCCAGCAGAAGGTTGTCCACGT        ACAGGAGCAAGATGCA GCCTA-3'
	3'-AAATC GTGTGGACTGACGACA       CCTCACAGGGTCGTCTTCCAACAGGTGCA        TGTCCTCGTTCTACGT CGGAT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 006-123066634
Forward Primer: 5'-GTAGTGGGATCGTGCCTGTG-3' T_m: 59.508
Reverse Primer: 5'-TCAGTACACTTGGACCCGGC-3' T_m: 59.508
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 135, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr6:123066737-123066797

Rank: 8	Homology Score: 17
	Genomic Region: Exon	Closest Gene: XPNPEP1
Match Type: R20R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-AGGGA TGCACCTGGATCCTGT            ATTCGGCTTCCAGACCCAAG            TCAAGAAGCAGGTGCT CCTTC-3'
	3'-TCCCT ACGTGGACCTAGGACA            TAAGCCGAAGGTCTGGGTTC            AGTTCTTCGTCCACGA GGAAG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 010-111642202
Forward Primer: 5'-CTCGCTCACAGCATAGCTGG-3' T_m: 59.508
Reverse Primer: 5'-TATGTGTGTCTGTGTTGCCACCTC-3' T_m: 59.038
Expected PCR Product Length: 273
Expected CelI Cleavage Product Sizes: 131, 142
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr10:111642305-111642356

Rank: 9	Homology Score: 17
	Genomic Region: Exon	Closest Gene: LOC390660
Match Type: R27R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CTGCC CGCCGCTGCCACCTGT        GGCCCTGTGCCACCTGGCACTCAGGCA         ACTGGAGTAAGGTGCA TGAGG-3'
	3'-GACGG GCGGCGACGGTGGACA        CCGGGACACGGTGGACCGTGAGTCCGT         TGACCTCATTCCACGT ACTCC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 015-082621505
Forward Primer: 5'-TGGCTTTCACGGGTCTGTGC-3' T_m: 59.508
Reverse Primer: 5'-TAGACATGCAGCCTGGAGCC-3' T_m: 59.508
Expected PCR Product Length: 296
Expected CelI Cleavage Product Sizes: 157, 139
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr15:82621631-82621689

Rank: 10	Homology Score: 17
	Genomic Region: Exon	Closest Gene: LOC390660
Match Type: R27R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CTGCC CGCCGCTGCCACCTGT        GGCCCTGTGCCACCTGGCACTCAGGCA         ACTGGAGTAAGGTGCA TGAGG-3'
	3'-GACGG GCGGCGACGGTGGACA        CCGGGACACGGTGGACCGTGAGTCCGT         TGACCTCATTCCACGT ACTCC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 015-082918832
Forward Primer: 5'-TGGCTTTCACGGGTCTGTGC-3' T_m: 59.508
Reverse Primer: 5'-TAGACATGCAGCCTGGAGCC-3' T_m: 59.508
Expected PCR Product Length: 296
Expected CelI Cleavage Product Sizes: 157, 139
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr15:82918958-82919016

Rank: 11	Homology Score: 17
	Genomic Region: Intron	Closest Gene: MYLK
Match Type: R12R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-ACTAC TGCACCTGGCTTCTGT                GTTGTGTTTCAA                GCAGCAGACAGATGCC CCGAC-3'
	3'-TGATG ACGTGGACCGAAGACA                CAACACAAAGTT                CGTCGTCTGTCTACGG GGCTG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 003-123352705
Forward Primer: 5'-GGTTTTAAGAAATGGGGGTCTCGC-3' T_m: 59.038
Reverse Primer: 5'-TCCCACCAGAGTATCAGATGGC-3' T_m: 59.258
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 138, 132
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr3:123352819-123352862

Rank: 12	Homology Score: 17
	Genomic Region: Intron	Closest Gene: PARD3B
Match Type: R30R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GGAGT AGAAACTGATTCCTAT       GCAAGCAAAAATAACATAGGGGAACTGACC       ACAGGAGTTAGGTGGA GTTAG-3'
	3'-CCTCA TCTTTGACTAAGGATA       CGTTCGTTTTTATTGTATCCCCTTGACTGG       TGTCCTCAATCCACCT CAATC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 002-205984816
Forward Primer: 5'-GTGGGTACTGGCTGCTAAGTATG-3' T_m: 59.148
Reverse Primer: 5'-GTTCCTAGCAGCCACAGCAC-3' T_m: 59.508
Expected PCR Product Length: 428
Expected CelI Cleavage Product Sizes: 256, 172
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr2:205985039-205985100

Rank: 13	Homology Score: 17
	Genomic Region: Intron	Closest Gene: CABIN1
Match Type: R10R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GGGGT TGCATCTGAATCCTGT                 GGGTGTGGCA                 GCAGGGGACAGCTGGA AAAGG-3'
	3'-CCCCA ACGTAGACTTAGGACA                 CCCACACCGT                 CGTCCCCTGTCGACCT TTTCC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 022-024555541
Forward Primer: 5'-CACAGCCATTCTGTCTACTCCC-3' T_m: 59.258
Reverse Primer: 5'-TGGCAGGTGGCCAGTGTCTT-3' T_m: 59.508
Expected PCR Product Length: 273
Expected CelI Cleavage Product Sizes: 140, 133
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr22:24555658-24555699

Rank: 14	Homology Score: 17
	Genomic Region: Intron	Closest Gene: CARD11
Match Type: R17R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GGGGG TGCACCTGCCTCCTGC             CTGCACCCTGGGAGCTT              GCAGGATGCAGAAGCA TGGGC-3'
	3'-CCCCC ACGTGGACGGAGGACG             GACGTGGGACCCTCGAA              CGTCCTACGTCTTCGT ACCCG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 007-002962408
Forward Primer: 5'-ACAGCGTTTGTCTCCTGGCC-3' T_m: 59.508
Reverse Primer: 5'-GCCCCTAAGCCTTGCATTCAG-3' T_m: 59.378
Expected PCR Product Length: 298
Expected CelI Cleavage Product Sizes: 167, 131
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr7:2962549-2962597

Rank: 15	Homology Score: 17
	Genomic Region: Intron	Closest Gene: ATRN
Match Type: R12R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GCCAC TGCACCTGACCCCTAT                TATCTTCTTTTG                AAATGAGTCATCTGCT AATTC-3'
	3'-CGGTG ACGTGGACTGGGGATA                ATAGAAGAAAAC                TTTACTCAGTAGACGA TTAAG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 020-003459687
Forward Primer: 5'-GCCAGTGTGCCCAGCCAATT-3' T_m: 59.508
Reverse Primer: 5'-GTGTACGTAGCTGTCTTCCCTTC-3' T_m: 59.148
Expected PCR Product Length: 323
Expected CelI Cleavage Product Sizes: 164, 159
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr20:3459827-3459870

Rank: 16	Homology Score: 17
	Genomic Region: Intron	Closest Gene: SDK1
Match Type: R13R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TGTTT TGAACCTGACTCCTTT               CACATGGCATAGC                ACATGTGACACATGCA TATCA-3'
	3'-ACAAA ACTTGGACTGAGGAAA               GTGTACCGTATCG                TGTACACTGTGTACGT ATAGT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 007-003795640
Forward Primer: 5'-CCTGACTCCTTTCACATGGCATAG-3' T_m: 59.038
Reverse Primer: 5'-TGTGTCACACGTGCTATGCCATG-3' T_m: 59.148
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 135, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr7:3795751-3795795

Rank: 17	Homology Score: 17
	Genomic Region: Intron	Closest Gene: SDK1
Match Type: R13R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TGTTT TGAACCTGACTCCTTT               CACATGGCATAGC                ACATGTGACACATGCA TATCA-3'
	3'-ACAAA ACTTGGACTGAGGAAA               GTGTACCGTATCG                TGTACACTGTGTACGT ATAGT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 007-003795876
Forward Primer: 5'-GAGTCCTTTCACATGGCATAGCAC-3' T_m: 59.038
Reverse Primer: 5'-ACATGTCTCACACGCACAATGCC-3' T_m: 59.148
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 135, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr7:3795987-3796031

Rank: 18	Homology Score: 17
	Genomic Region: Intron	Closest Gene: PAK4
Match Type: R27R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TTGTC CTCCCCTTGCTCCTGT        CCATTCCGACTCTCACCCATTCTGTGG         ACAGGAGTCAAGTGCT TATTC-3'
	3'-AACAG GAGGGGAACGAGGACA        GGTAAGGCTGAGAGTGGGTAAGACACC         TGTCCTCAGTTCACGA ATAAG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 019-039625781
Forward Primer: 5'-CCTCACCAACATTTGGTACCGTC-3' T_m: 59.148
Reverse Primer: 5'-CGGAGGTTGCAGTGAGCTGA-3' T_m: 59.508
Expected PCR Product Length: 282
Expected CelI Cleavage Product Sizes: 151, 131
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr19:39625901-39625959

Rank: 19	Homology Score: 17
	Genomic Region: Intergenic	Closest Gene: GRIK2
Match Type: R29R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CTTGG TGCACCCGACTGCTGT       GGGGTGTCCCAGCAGGAGGTTGTCAATGT        ACTGGAGCAACATGCA ACCTA-3'
	3'-GAACC ACGTGGGCTGACGACA       CCCCACAGGGTCGTCCTCCAACAGTTACA        TGACCTCGTTGTACGT TGGAT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 006-101676033
Forward Primer: 5'-CCAGGTAACATACTTGCTGTCTGC-3' T_m: 59.038
Reverse Primer: 5'-GTCACTACTCAGTACACTTGGACC-3' T_m: 59.038
Expected PCR Product Length: 275
Expected CelI Cleavage Product Sizes: 131, 144
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr6:101676132-101676192

Rank: 20	Homology Score: 17
	Genomic Region: Intergenic	Closest Gene: GSTM3
Match Type: R15R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CCTAC TGCTGCTGTCTCCTTC              TAATTCTGTCGGACA               ACAGGAGTCAGGTTCT GAGTC-3'
	3'-GGATG ACGACGACAGAGGAAG              ATTAAGACAGCCTGT               TGTCCTCAGTCCAAGA CTCAG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 001-110268884
Forward Primer: 5'-CAGGCAGGCAATCTTGATGTGG-3' T_m: 59.258
Reverse Primer: 5'-CTCCTTTTTCCTGCGTAGCAAGTG-3' T_m: 59.038
Expected PCR Product Length: 283
Expected CelI Cleavage Product Sizes: 152, 131
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr1:110269011-110269057

Rank: 21	Homology Score: 17
	Genomic Region: Intergenic	Closest Gene: GPR6
Match Type: R30R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CCATC TCCACCTGGCTCCTGT       GTGGTCCATGTTGCAAGTTTTCAGGAAGGA       GCAGGAGTTAAGGGTA GAGTT-3'
	3'-GGTAG AGGTGGACCGAGGACA       CACCAGGTACAACGTTCAAAAGTCCTTCCT       CGTCCTCAATTCCCAT CTCAA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 006-110284189
Forward Primer: 5'-GCAATGTTGCTCCAAGAGGCC-3' T_m: 59.378
Reverse Primer: 5'-GAACTTATTCCTCCCACCTAGCTG-3' T_m: 59.038
Expected PCR Product Length: 297
Expected CelI Cleavage Product Sizes: 131, 166
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr6:110284287-110284348

Rank: 22	Homology Score: 17
	Genomic Region: Intergenic	Closest Gene: SH3BP4
Match Type: R23R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GCAAT TGCACCTCACTTCTGT          GGAGTGCTTCCTGTGTATCAGGC           ACAGAAGCCCGATGTA TTCTC-3'
	3'-CGTTA ACGTGGAGTGAAGACA          CCTCACGAAGGACACATAGTCCG           TGTCTTCGGGCTACAT AAGAG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 002-235805535
Forward Primer: 5'-ACCTCCACCAGACCACGAATG-3' T_m: 59.378
Reverse Primer: 5'-GACCCTTAGCAGGTCACACTC-3' T_m: 59.378
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 135, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr2:235805641-235805695

Rank: 23	Homology Score: 17
	Genomic Region: Intergenic	Closest Gene: SLC2A10
Match Type: R24R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CCCCT TCCTCCAGCTTCCTGT          AGGTTCAACTAGGGGGAAGCCCCA          GCAGGAGACAGGTGCA CAGTG-3'
	3'-GGGGA AGGAGGTCGAAGGACA          TCCAAGTTGATCCCCCTTCGGGGT          CGTCCTCTGTCCACGT GTCAC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 020-045428486
Forward Primer: 5'-GTCTCACTGAGACCTAGGCG-3' T_m: 59.508
Reverse Primer: 5'-TGGGCCTGGGGAACCAACAA-3' T_m: 59.508
Expected PCR Product Length: 268
Expected CelI Cleavage Product Sizes: 133, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr20:45428589-45428644

Rank: 24	Homology Score: 17
	Genomic Region: Intergenic	Closest Gene: TRHDE-AS1
Match Type: R28R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CCTCC TAGACCTGACTCCTGT        ATTTGTTTCCTCACACTTAAAGGGAACT        CAATGAGTCAGGTGAG TACTG-3'
	3'-GGAGG ATCTGGACTGAGGACA        TAAACAAAGGAGTGTGAATTTCCCTTGA        GTTACTCAGTCCACTC ATGAC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Warning! Primer pair was generated using suboptimal parameters. 
Primer Index: 012-072604585
Forward Primer: 5'-GTTGAGAGTTGACACCAACTTCTTTTCTTTTTCC-3' T_m: 59.668
Reverse Primer: 5'-CCACTCCTAGTGTGGAACCTC-3' T_m: 59.378
Expected PCR Product Length: 393
Expected CelI Cleavage Product Sizes: 263, 130
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr12:72604816-72604875

Rank: 25	Homology Score: 17
	Genomic Region: Intergenic	Closest Gene: LOC642423
Match Type: R27R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CCTCA TGCACCTTACTCCAGT        TGCCTGAGTGCCAGGTGGCACAGGGCC         ACAGGTGGCAGCGGCG GGCAG-3'
	3'-GGAGT ACGTGGAATGAGGTCA        ACGGACTCACGGTCCACCGTGTCCCGG         TGTCCACCGTCGCCGC CCGTC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 015-085803797
Forward Primer: 5'-TAGACATGCAGCCTGGAGCC-3' T_m: 59.508
Reverse Primer: 5'-TGGCTTTCACGGGTCTGTGC-3' T_m: 59.508
Expected PCR Product Length: 296
Expected CelI Cleavage Product Sizes: 142, 154
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr15:85803908-85803966

Rank: 26	Homology Score: 13
	Genomic Region: Exon	Closest Gene: CD300LD
Match Type: R16R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CCACA TGGTCCTGTCTCCTCT              CCTCTCCTTGGTGATC              ACAGGTGTCTGGTGCC CTTCA-3'
	3'-GGTGT ACCAGGACAGAGGAGA              GGAGAGGAACCACTAG              TGTCCACAGACCACGG GAAGT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 017-072588236
Forward Primer: 5'-CAGAGCGTCTCTGTCTCCAAG-3' T_m: 59.378
Reverse Primer: 5'-GGCACACCTTGCAACATACACTG-3' T_m: 59.148
Expected PCR Product Length: 329
Expected CelI Cleavage Product Sizes: 131, 198
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr17:72588341-72588388

Rank: 27	Homology Score: 13
	Genomic Region: Intron	Closest Gene: HPSE2
Match Type: R14R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CAGGC TCCACATGCCTCCTGT               GTGAAATCCCCCCA               ACAAGACTCAGATGCC TCAGG-3'
	3'-GTCCG AGGTGTACGGAGGACA               CACTTTAGGGGGGT               TGTTCTGAGTCTACGG AGTCC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 010-100332368
Forward Primer: 5'-GAGAGTGAACTCACCTTCCATGAC-3' T_m: 59.038
Reverse Primer: 5'-TGGGCACACAGTCAGTTCCTAG-3' T_m: 59.258
Expected PCR Product Length: 304
Expected CelI Cleavage Product Sizes: 174, 130
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr10:100332517-100332562

Rank: 28	Homology Score: 13
	Genomic Region: Intron	Closest Gene: RALGPS1
Match Type: R22R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TGAGC TGCGCCTGAGTCCAGG           CCTTGCTATTCCTGAGATAGAA           AACGGAGTCAGATGCA GAGCT-3'
	3'-ACTCG ACGCGGACTCAGGTCC           GGAACGATAAGGACTCTATCTT           TTGCCTCAGTCTACGT CTCGA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 009-129960176
Forward Primer: 5'-CTTCCTTGGAGTGTCCAATGGG-3' T_m: 59.258
Reverse Primer: 5'-CATCAGGGCTTTTGGCCTCAAAC-3' T_m: 59.148
Expected PCR Product Length: 278
Expected CelI Cleavage Product Sizes: 148, 130
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr9:129960295-129960348

Rank: 29	Homology Score: 13
	Genomic Region: Intron	Closest Gene: GRIA2
Match Type: R19R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-ATCCA TACACCTGTCTGCTGT            AACCTTTTCCAGATTGGCT             ACTGGAGTGAAGTGGA CAAAA-3'
	3'-TAGGT ATGTGGACAGACGACA            TTGGAAAAGGTCTAACCGA             TGACCTCACTTCACCT GTTTT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Acceptable Primer Pair Not Found!
Left Primers Found: 53
Right Primers Found: 119
Primers may not have been generated due to repetitive genomic sequence.
Primer pairs may not have been generated since primer dimers would form or product sizes are incompatible with specified parameters.
A careful manual examination of the genomic sequence may allow design of primers suitable for amplification of desired product.
View in Genome Browser, Chromosomal Coordinates - chr4:158255134-158255184

Rank: 30	Homology Score: 13
	Genomic Region: Intron	Closest Gene: ERGIC1
Match Type: R10R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-AGGTC TGCTCCTGCCTCCAGT                 AGAAGCAGCC                 ACAGGAGTCCTGGCCA CAGCC-3'
	3'-TCCAG ACGAGGACGGAGGTCA                 TCTTCGTCGG                 TGTCCTCAGGACCGGT GTCGG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 005-172280139
Forward Primer: 5'-TGGTTTACCACGTGCTGTTGTTGC-3' T_m: 59.038
Reverse Primer: 5'-AAGATGCCTCTGGGTGGCCT-3' T_m: 59.508
Expected PCR Product Length: 272
Expected CelI Cleavage Product Sizes: 131, 141
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr5:172280247-172280288

Rank: 31	Homology Score: 13
	Genomic Region: Intron	Closest Gene: SORBS2
Match Type: R12R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-AATGT TTTTCCTGACTTCTGT                GATGTATAGGGC                ACTGTAGTCAGGTGAA ATAAC-3'
	3'-TTACA AAAAGGACTGAAGACA                CTACATATCCCG                TGACATCAGTCCACTT TATTG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 004-186747672
Forward Primer: 5'-CTGTGAGCTACTGAGGATATGCG-3' T_m: 59.148
Reverse Primer: 5'-GGGCAGATGCTTAAATCACTCCCA-3' T_m: 59.038
Expected PCR Product Length: 374
Expected CelI Cleavage Product Sizes: 218, 156
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr4:186747866-186747909

Rank: 32	Homology Score: 13
	Genomic Region: Intron	Closest Gene: SCRN1
Match Type: R10R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GTCAC TGTACCTGACTGATGT                 ATACTTTTTA                 TCATAAGCCAGGTGCA GTGGC-3'
	3'-CAGTG ACATGGACTGACTACA                 TATGAAAAAT                 AGTATTCGGTCCACGT CACCG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 007-030006854
Forward Primer: 5'-CCAAGAAGGTTATGCCCTGAATGC-3' T_m: 59.038
Reverse Primer: 5'-TAGTGGGTGTCCTTGGCTCTG-3' T_m: 59.378
Expected PCR Product Length: 433
Expected CelI Cleavage Product Sizes: 303, 130
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr7:30007134-30007175

Rank: 33	Homology Score: 13
	Genomic Region: Intron	Closest Gene: PBRM1
Match Type: R22R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TCTCC TGCAACTGAAGCCTGT           ATTTAATTTAAGCAGGAATGGC           ACTGGAATCAGGCTCA GCAGT-3'
	3'-AGAGG ACGTTGACTTCGGACA           TAAATTAAATTCGTCCTTACCG           TGACCTTAGTCCGAGT CGTCA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 003-052584959
Forward Primer: 5'-GGCTAGGAATCTTAAGCACCCC-3' T_m: 59.258
Reverse Primer: 5'-GAGGGGATAGCAAGGCTGTG-3' T_m: 59.508
Expected PCR Product Length: 378
Expected CelI Cleavage Product Sizes: 246, 132
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr3:52585176-52585229

Rank: 34	Homology Score: 13
	Genomic Region: Intron	Closest Gene: CACNA2D3
Match Type: R11R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CAAGG TGCCCCACACTCCTGT                GGTCTGGAAGG                 AATGGAGCCATGTGCA CACTT-3'
	3'-GTTCC ACGGGGTGTGAGGACA                CCAGACCTTCC                 TTACCTCGGTACACGT GTGAA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 003-054731501
Forward Primer: 5'-CCTACAGTTTCCAAGGACAAGCAG-3' T_m: 59.038
Reverse Primer: 5'-CCCAGCAGTAGCTTTAGCATCC-3' T_m: 59.258
Expected PCR Product Length: 320
Expected CelI Cleavage Product Sizes: 160, 160
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr3:54731638-54731680

Rank: 35	Homology Score: 13
	Genomic Region: Intron	Closest Gene: HYDIN
Match Type: R26R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CCCTC TGCACACAACTCCTAT         GCACTCTGCACGCACCACCCTCCTGC         ACAGGCGTCCCGTGCA GGCTG-3'
	3'-GGGAG ACGTGTGTTGAGGATA         CGTGAGACGTGCGTGGTGGGAGGACG         TGTCCGCAGGGCACGT CCGAC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 016-071012605
Forward Primer: 5'-CTCCTAGCTTCCTGATTCTTTGCC-3' T_m: 59.038
Reverse Primer: 5'-CCCACCCTGGTACAGCAAATG-3' T_m: 59.378
Expected PCR Product Length: 355
Expected CelI Cleavage Product Sizes: 168, 187
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr16:71012742-71012799

Rank: 36	Homology Score: 13
	Genomic Region: Intron	Closest Gene: C14orf166B
Match Type: R24R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TAACT TGCCCCCGACTCATGT          TTTCTTAGATTATCTCTGGCAATC          ACAGGAGGCAGAGGTA AAGCT-3'
	3'-ATTGA ACGGGGGCTGAGTACA          AAAGAATCTAATAGAGACCGTTAG          TGTCCTCCGTCTCCAT TTCGA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 014-077327606
Forward Primer: 5'-GCCTGGTATAGAACAAGCACCTG-3' T_m: 59.148
Reverse Primer: 5'-CCTCCTGTGATTGCCAGAGC-3' T_m: 59.508
Expected PCR Product Length: 368
Expected CelI Cleavage Product Sizes: 179, 189
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr14:77327755-77327810

Rank: 37	Homology Score: 13
	Genomic Region: Intron	Closest Gene: ACSS3
Match Type: R12R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TTGCA TGCACCCTAGTCCTGT                TGTGTGTTAATA                ACAGGCATCAATTGCA TTTTC-3'
	3'-AACGT ACGTGGGATCAGGACA                ACACACAATTAT                TGTCCGTAGTTAACGT AAAAG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Warning! Primer pair was generated using suboptimal parameters. 
Primer Index: 012-081520008
Forward Primer: 5'-GGCAGCAACTCTCCAGTGAGTA-3' T_m: 59.258
Reverse Primer: 5'-CCTGTATTTCCCTATGTATATGTGCTCAGTC-3' T_m: 59.958
Expected PCR Product Length: 413
Expected CelI Cleavage Product Sizes: 171, 242
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr12:81520155-81520198

Rank: 38	Homology Score: 13
	Genomic Region: Intron	Closest Gene: RNF103-CHMP3
Match Type: R19R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-AGTGA TCCTCCTGCCTCCTGT            CCCCCAAAATGCTGGGATT             ACAGGTGTGAGCTGCC ATGAC-3'
	3'-TCACT AGGAGGACGGAGGACA            GGGGGTTTTACGACCCTAA             TGTCCACACTCGACGG TACTG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Warning! Primer pair was generated using suboptimal parameters. 
Primer Index: 002-086899302
Forward Primer: 5'-GGCTCTGTCTTTTGTTATGTCAAATGAGAAGCA-3' T_m: 59.758
Reverse Primer: 5'-CTCCATCATTAATTCAAACAGCCTTCAATACCC-3' T_m: 59.758
Expected PCR Product Length: 373
Expected CelI Cleavage Product Sizes: 235, 138
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr2:86899510-86899560

Rank: 39	Homology Score: 13
	Genomic Region: Intergenic	Closest Gene: SLC25A47
Match Type: R28R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CCTGC TGCAGCTGGCTGTTGT        CCCCGTTCACAGATAGGGAAACCGAGGC        ACAGGAGTTAAGTCCA TTGGC-3'
	3'-GGACG ACGTCGACCGACAACA        GGGGCAAGTGTCTATCCCTTTGGCTCCG        TGTCCTCAATTCAGGT AACCG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 014-100782711
Forward Primer: 5'-TGAAGGGACAGAGCCAGGGT-3' T_m: 59.508
Reverse Primer: 5'-ACACCAGCCTGGGCAACAGA-3' T_m: 59.508
Expected PCR Product Length: 286
Expected CelI Cleavage Product Sizes: 156, 130
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr14:100782835-100782894

Rank: 40	Homology Score: 13
	Genomic Region: Intergenic	Closest Gene: GAS2L3
Match Type: R29R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TTTTG CGCACCCGACTGCCGT       GGAGTGTTCCAGCAGAAGGTCGTCCACGT        ACAGGAGCTAGATGCA TCCTG-3'
	3'-AAAAC GCGTGGGCTGACGGCA       CCTCACAAGGTCGTCTTCCAGCAGGTGCA        TGTCCTCGATCTACGT AGGAC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 012-101038644
Forward Primer: 5'-CTGTGCTCCCCTTTCTGAATAGTG-3' T_m: 59.038
Reverse Primer: 5'-TACACTTGGACCCGACTTCCC-3' T_m: 59.378
Expected PCR Product Length: 272
Expected CelI Cleavage Product Sizes: 141, 131
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr12:101038753-101038813

Rank: 41	Homology Score: 13
	Genomic Region: Intergenic	Closest Gene: CTSB
Match Type: R29R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CTTGG TGCACCTGACTGCCAT       GGGGTGTCCCAGCAAAAGGGCATCAATCT        ACTGGAGCCACATGCA GTCTA-3'
	3'-GAACC ACGTGGACTGACGGTA       CCCCACAGGGTCGTTTTCCCGTAGTTAGA        TGACCTCGGTGTACGT CAGAT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 008-011765812
Forward Primer: 5'-ATCCCCAGTAATGTACCTGCTGTC-3' T_m: 59.038
Reverse Primer: 5'-CTCAGTACACTTGCACCCGG-3' T_m: 59.508
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 134, 136
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr8:11765914-11765974

Rank: 42	Homology Score: 13
	Genomic Region: Intergenic	Closest Gene: SGCZ
Match Type: R10R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-ATGGA TGCTCCTGCCACCTGT                 GGAGCCAGCC                 TCAGGAGGCAGGTGAG GGAGG-3'
	3'-TACCT ACGAGGACGGTGGACA                 CCTCGGTCGG                 AGTCCTCCGTCCACTC CCTCC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 008-015147764
Forward Primer: 5'-CCCCACTTCATCTGCTAAACTTCC-3' T_m: 59.038
Reverse Primer: 5'-GAGGGGGAAAATGTGGGAAGTTCT-3' T_m: 59.038
Expected PCR Product Length: 313
Expected CelI Cleavage Product Sizes: 168, 145
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr8:15147909-15147950

Rank: 43	Homology Score: 13
	Genomic Region: Intergenic	Closest Gene: FAM41AY1
Match Type: R29R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TAGGC TGCATCTTACTCCAGT       ACATAGACAACCTTCTGCTAGGATACTCC        ACAGCAGTCGGGTGTG CAAGA-3'
	3'-ATCCG ACGTAGAATGAGGTCA       TGTATCTGTTGGAAGACGATCCTATGAGG        TGTCGTCAGCCCACAC GTTCT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 00Y-018946879
Forward Primer: 5'-ATACACTTGGACTCGGCTTCCC-3' T_m: 59.258
Reverse Primer: 5'-GTGAATCTCAGGTAGTGTACCTGC-3' T_m: 59.038
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 135, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chrY:18946982-18947042

Rank: 44	Homology Score: 13
	Genomic Region: Intergenic	Closest Gene: FRG1
Match Type: R14R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GTTTC TACCCCTGACCCCTGC               AGTCTACTCTGAGC               ACAGCAGTCAGAAGCA TTCTT-3'
	3'-CAAAG ATGGGGACTGGGGACG               TCAGATGAGACTCG               TGTCGTCAGTCTTCGT AAGAA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 004-190659833
Forward Primer: 5'-CTGCCTCCAATCCCTCAGAAAATG-3' T_m: 59.038
Reverse Primer: 5'-CTGGTATTGGGTCCAGACCAC-3' T_m: 59.378
Expected PCR Product Length: 308
Expected CelI Cleavage Product Sizes: 178, 130
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr4:190659986-190660031

Rank: 45	Homology Score: 13
	Genomic Region: Intergenic	Closest Gene: PLCL1
Match Type: R13R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GCAGA TACACCTGACTTCTAT               TTGAGGCCCAGAT                ACTGGAGACAGGAGCC TTATT-3'
	3'-CGTCT ATGTGGACTGAAGATA               AACTCCGGGTCTA                TGACCTCTGTCCTCGG AATAA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 002-199208411
Forward Primer: 5'-GCCTTACACATAACCTCACTGCC-3' T_m: 59.148
Reverse Primer: 5'-CTCCAACATAACTCAGCCTCGAAG-3' T_m: 59.038
Expected PCR Product Length: 393
Expected CelI Cleavage Product Sizes: 166, 227
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr2:199208553-199208597

Rank: 46	Homology Score: 13
	Genomic Region: Intergenic	Closest Gene: HYDIN2
Match Type: R26R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CCCTC TGCACACAACTCCTAT         GCACTCTGCACGCACCACCCTCCTGC         ACAGGTGTCCCGTGCA GGCTG-3'
	3'-GGGAG ACGTGTGTTGAGGATA         CGTGAGACGTGCGTGGTGGGAGGACG         TGTCCACAGGGCACGT CCGAC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: dom-000216374
Forward Primer: 5'-TCTCCTTTCTGTACCTCAGAGACC-3' T_m: 59.038
Reverse Primer: 5'-CCCACCCTGGTACAGCAAATG-3' T_m: 59.378
Expected PCR Product Length: 318
Expected CelI Cleavage Product Sizes: 131, 187
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr1_gl000192_random:216474-216531

Rank: 47	Homology Score: 13
	Genomic Region: Intergenic	Closest Gene: GPC1
Match Type: R29R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GAGCC TTCACCTGATTCCAGC       CGCAAAGCCCTTTCCAGTACGGCCCATTC        ACAGGTTCCAGGTGCA AAGCC-3'
	3'-CTCGG AAGTGGACTAAGGTCG       GCGTTTCGGGAAAGGTCATGCCGGGTAAG        TGTCCAAGGTCCACGT TTCGG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 002-241334168
Forward Primer: 5'-CATGCACCCCTGGCTTGTGT-3' T_m: 59.508
Reverse Primer: 5'-AGCTTTGCACCTGGAACCTGTGA-3' T_m: 59.148
Expected PCR Product Length: 297
Expected CelI Cleavage Product Sizes: 138, 159
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr2:241334274-241334334

Rank: 48	Homology Score: 13
	Genomic Region: Intergenic	Closest Gene: RNU6-78
Match Type: R22R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TGAGT ATCACCTCACACCTGT           TAGGATGGCTATTATAAAAAAG           GCAGCAGCCAGGTGCA GTGGC-3'
	3'-ACTCA TAGTGGAGTGTGGACA           ATCCTACCGATAATATTTTTTC           CGTCGTCGGTCCACGT CACCG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 012-032161181
Forward Primer: 5'-GCTCAATGGCAGAAAACCAAATAACCCAAC-3' T_m: 60.058
Reverse Primer: 5'-GAGACGGGTTTTTGCCATGTTGC-3' T_m: 59.148
Expected PCR Product Length: 315
Expected CelI Cleavage Product Sizes: 185, 130
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr12:32161337-32161390

Rank: 49	Homology Score: 13
	Genomic Region: Intergenic	Closest Gene: ATP8B1
Match Type: R11R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-AGGGC TGCACGTGACTTCTGG                GGCAAAGGTAA                 GTAGGAGAAAGGTGCA CCTTC-3'
	3'-TCCCG ACGTGCACTGAAGACC                CCGTTTCCATT                 CATCCTCTTTCCACGT GGAAG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 018-055555293
Forward Primer: 5'-TCTGGGAAGAAGCTGCCAGG-3' T_m: 59.508
Reverse Primer: 5'-GGCTATGCTACCTGCTGTAAAACC-3' T_m: 59.038
Expected PCR Product Length: 342
Expected CelI Cleavage Product Sizes: 134, 208
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr18:55555404-55555446

Rank: 50	Homology Score: 13
	Genomic Region: Intergenic	Closest Gene: FAM69C
Match Type: R26R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CCTAG TTCACTTCACTCCTGT         TATCCACTAAGCTCATTATCACATCG         CCAGAAGTGAGGTCCA TTTGT-3'
	3'-GGATC AAGTGAAGTGAGGACA         ATAGGTGATTCGAGTAATAGTGTAGC         GGTCTTCACTCCAGGT AAACA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 018-072086920
Forward Primer: 5'-GCTGTCACCAGGAAAAACATAAGAGCC-3' T_m: 60.438
Reverse Primer: 5'-AAGCTGCTGAAATGGGCAGAGG-3' T_m: 59.258
Expected PCR Product Length: 289
Expected CelI Cleavage Product Sizes: 159, 130
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr18:72087048-72087105

Rank: 51	Homology Score: 13
	Genomic Region: Intergenic	Closest Gene: ZNF812
Match Type: R29R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TTTTG TGCACCCAACTGCTGT       GGAGTGTCCCAGCAGAACGTCGTCCATGT        ACAGGAGCAAGACGCA GCCTA-3'
	3'-AAAAC ACGTGGGTTGACGACA       CCTCACAGGGTCGTCTTGCAGCAGGTACA        TGTCCTCGTTCTGCGT CGGAT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 019-009837811
Forward Primer: 5'-AATCCCAGGTAGCGTACGTGC-3' T_m: 59.378
Reverse Primer: 5'-TCAGTACACATGGACCTGGCTTC-3' T_m: 59.148
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 135, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr19:9837914-9837974

Rank: 52	Homology Score: 10
	Genomic Region: Exon	Closest Gene: TMEM132C
Match Type: R27R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TCGTG ATCACCTGACTCCAGT        TCTCCGTGTGCTCCATTGGCTGCGGCT         GCAGGAGGAAGATGCC TGACA-3'
	3'-AGCAC TAGTGGACTGAGGTCA        AGAGGCACACGAGGTAACCGACGCCGA         CGTCCTCCTTCTACGG ACTGT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 012-129191997
Forward Primer: 5'-GGTGGGGTATACCAGCATAAACTC-3' T_m: 59.038
Reverse Primer: 5'-GGCTTCAAGGACCCTTTCTTAGCT-3' T_m: 59.038
Expected PCR Product Length: 337
Expected CelI Cleavage Product Sizes: 197, 140
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr12:129192163-129192221

Rank: 53	Homology Score: 10
	Genomic Region: Exon	Closest Gene: KIF20A
Match Type: R14R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CATCT TACACCCCTCTCCTTT               GGCCCCAGATCTCA               ACTGGATTCATGTGCA AGATG-3'
	3'-GTAGA ATGTGGGGAGAGGAAA               CCGGGGTCTAGAGT               TGACCTAAGTACACGT TCTAC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 005-137519020
Forward Primer: 5'-GCGAGGATCAAAATGGCAATCCC-3' T_m: 59.148
Reverse Primer: 5'-GGCAGAGGACTAATGTTTCCTGG-3' T_m: 59.148
Expected PCR Product Length: 310
Expected CelI Cleavage Product Sizes: 140, 170
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr5:137519135-137519180

Rank: 54	Homology Score: 10
	Genomic Region: Exon	Closest Gene: KIAA1244
Match Type: R19R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CATTG TGTAGCCGACTCCTGT            TCTACTCTCCCACCAAATA             ACAGTAGTGAGGGTTA GAGTC-3'
	3'-GTAAC ACATCGGCTGAGGACA            AGATGAGAGGGTGGTTTAT             TGTCATCACTCCCAAT CTCAG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 006-138657515
Forward Primer: 5'-GTTCCCGTGCATCAGTCAGC-3' T_m: 59.508
Reverse Primer: 5'-GCTGATCAGTGGTTGGAAAGGC-3' T_m: 59.258
Expected PCR Product Length: 272
Expected CelI Cleavage Product Sizes: 131, 141
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr6:138657619-138657669

Rank: 55	Homology Score: 10
	Genomic Region: Exon	Closest Gene: NOL6
Match Type: R28R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-ACCGT TGCACCTCACCTCTGT        GTAGCGCAGCACTGGGTGAGCTCCCTGA        ACAGCAGACACGGTCA GTGGG-3'
	3'-TGGCA ACGTGGAGTGGAGACA        CATCGCGTCGTGACCCACTCGAGGGACT        TGTCGTCTGTGCCAGT CACCC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 009-033466447
Forward Primer: 5'-AATGGGCCTAGGACTGGGAG-3' T_m: 59.508
Reverse Primer: 5'-TCTCCCTCTCAGACCTCCAG-3' T_m: 59.508
Expected PCR Product Length: 273
Expected CelI Cleavage Product Sizes: 142, 131
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr9:33466557-33466616

Rank: 56	Homology Score: 10
	Genomic Region: Exon	Closest Gene: NCDN
Match Type: R22R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-ACCTG AGCATCTGATTCCTGT           CCCCCTGGTGCCATCTGGCCTG           GCTGGAGCCAGGAACA GGAGG-3'
	3'-TGGAC TCGTAGACTAAGGACA           GGGGGACCACGGTAGACCGGAC           CGACCTCGGTCCTTGT CCTCC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 001-036031906
Forward Primer: 5'-CAGAGTCTTTGGGCCCAGATC-3' T_m: 59.378
Reverse Primer: 5'-TGCAGAGAGGAGTCATAGCAGG-3' T_m: 59.258
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 140, 130
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr1:36032017-36032070

Rank: 57	Homology Score: 10
	Genomic Region: Exon	Closest Gene: AMIGO2
Match Type: R15R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GCAGA AGTACCTGACGCGAGT              GCCTGGAGTCAGACC               ACAGGCGACAGGTGTA ATCGT-3'
	3'-CGTCT TCATGGACTGCGCTCA              CGGACCTCAGTCTGG               TGTCCGCTGTCCACAT TAGCA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 012-047471859
Forward Primer: 5'-GTGGACCATCAGTCTTTCCCC-3' T_m: 59.378
Reverse Primer: 5'-CTGAGAGGCATCTACCTTCATGG-3' T_m: 59.148
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 134, 136
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr12:47471968-47472014

Rank: 58	Homology Score: 10
	Genomic Region: Exon	Closest Gene: DCHS1
Match Type: R23R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GCTCC TGCACCTGGCAGCAGC          AGCCCTGCCTTTCGGCCCTTATA           CCAGGTGTCAGGGGCA GGTGG-3'
	3'-CGAGG ACGTGGACCGTCGTCG          TCGGGACGGAAAGCCGGGAATAT           GGTCCACAGTCCCCGT CCACC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 011-006643499
Forward Primer: 5'-TGGGAAGCCATAGTCCCCAG-3' T_m: 59.508
Reverse Primer: 5'-ATCAATGAGTTCCCCCGTGTGG-3' T_m: 59.258
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 131, 139
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr11:6643601-6643655

Rank: 59	Homology Score: 10
	Genomic Region: Exon	Closest Gene: C10orf99
Match Type: R17R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-AGGTA CCCAGCAGCCTCCTGT             CTCCCCTTTCAGCCTTC              ACAGCAGTGAGCTGCA ATGTT-3'
	3'-TCCAT GGGTCGTCGGAGGACA             GAGGGGAAAGTCGGAAG              TGTCGTCACTCGACGT TACAA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 010-085944463
Forward Primer: 5'-TGCAAGCTTGAGCCAGAGCC-3' T_m: 59.508
Reverse Primer: 5'-GAGGTTGGCTCATGATAGCTCTG-3' T_m: 59.148
Expected PCR Product Length: 274
Expected CelI Cleavage Product Sizes: 142, 132
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr10:85944579-85944627

Rank: 60	Homology Score: 10
	Genomic Region: Promoter	Closest Gene: C1orf198
Match Type: R11R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CTCTC TCCATCTGACTGATTT                TAACAAGACTA                 ACAGGACTCAGGTGAG AATTG-3'
	3'-GAGAG AGGTAGACTGACTAAA                ATTGTTCTGAT                 TGTCCTGAGTCCACTC TTAAC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 001-231005091
Forward Primer: 5'-GCGACATCCCAACAGGTAGG-3' T_m: 59.508
Reverse Primer: 5'-CGGAAGATGAGGCCATCTCAC-3' T_m: 59.378
Expected PCR Product Length: 298
Expected CelI Cleavage Product Sizes: 131, 167
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr1:231005199-231005241

Rank: 61	Homology Score: 10
	Genomic Region: Promoter	Closest Gene: MAMSTR
Match Type: R25R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CTACC GGCAGGTGCCACCTGT         CGCCTACTTTTTCCCTGACGCAGCA          ACTGGAGTCAAGTTCA GGGGA-3'
	3'-GATGG CCGTCCACGGTGGACA         GCGGATGAAAAAGGGACTGCGTCGT          TGACCTCAGTTCAAGT CCCCT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 019-049223033
Forward Primer: 5'-AACCCACCTTCCTGGGGTGT-3' T_m: 59.508
Reverse Primer: 5'-GAGGCCCCTGCCTCTTTTTC-3' T_m: 59.508
Expected PCR Product Length: 423
Expected CelI Cleavage Product Sizes: 292, 131
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr19:49223295-49223351

Rank: 62	Homology Score: 10
	Genomic Region: Intron	Closest Gene: NPAS2
Match Type: R14R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GGTTG TGCACCTGTCTCCAGA               CTCTAAGCCTGGGC               ACAGGAGGGGACTGCA CTGAC-3'
	3'-CCAAC ACGTGGACAGAGGTCT               GAGATTCGGACCCG               TGTCCTCCCCTGACGT GACTG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 002-101527279
Forward Primer: 5'-CATGCCCAGCCTAATGTCTTCTTC-3' T_m: 59.038
Reverse Primer: 5'-GCCAGAGGAGGTTATTTTCTTGCC-3' T_m: 59.038
Expected PCR Product Length: 427
Expected CelI Cleavage Product Sizes: 148, 279
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr2:101527402-101527447

Rank: 63	Homology Score: 10
	Genomic Region: Intron	Closest Gene: PITX3
Match Type: R20R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CGTCC TGAACCTGAGTCCTGG            GCCAATGTTCCCATCCCTGA            GCAGGAGGCAGGCAGA GATAT-3'
	3'-GCAGG ACTTGGACTCAGGACC            CGGTTACAAGGGTAGGGACT            CGTCCTCCGTCCGTCT CTATA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 010-103998000
Forward Primer: 5'-CTTCAGGTTAGTCTAGGAGGCATC-3' T_m: 59.038
Reverse Primer: 5'-TGACAGACGGGCCACCCTAT-3' T_m: 59.508
Expected PCR Product Length: 281
Expected CelI Cleavage Product Sizes: 144, 137
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr10:103998116-103998167

Rank: 64	Homology Score: 10
	Genomic Region: Intron	Closest Gene: GRIA4
Match Type: R21R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TATGT TGCACATGATTCCTTT           TAATCAACTCAACTGGAAGCC            AAAGCACACAGCTGCA GACCA-3'
	3'-ATACA ACGTGTACTAAGGAAA           ATTAGTTGAGTTGACCTTCGG            TTTCGTGTGTCGACGT CTGGT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 011-105719867
Forward Primer: 5'-CAGATTGCGCCACTGCATTCC-3' T_m: 59.378
Reverse Primer: 5'-TGTCTCCTTCAGAACTGAGCTGG-3' T_m: 59.148
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 136, 134
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr11:105719975-105720027

Rank: 65	Homology Score: 10
	Genomic Region: Intron	Closest Gene: COL25A1
Match Type: R11R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TGCAA TGCAGCGGGCTCCCCT                TACACACCTTT                 ACAGGAGTTAGTTGAA TATCA-3'
	3'-ACGTT ACGTCGCCCGAGGGGA                ATGTGTGGAAA                 TGTCCTCAATCAACTT ATAGT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 004-110007835
Forward Primer: 5'-GACTGCTGGGAATTGCTCTTTGAC-3' T_m: 59.038
Reverse Primer: 5'-TCCCTCAACTTCCACTGGGC-3' T_m: 59.508
Expected PCR Product Length: 290
Expected CelI Cleavage Product Sizes: 157, 133
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr4:110007969-110008011

Rank: 66	Homology Score: 10
	Genomic Region: Intron	Closest Gene: PHTF1
Match Type: R11R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TATTG TGAACTTGACTACAGA                CCTACTCGTGA                 ACAGGATTCAGCTCCA AAACG-3'
	3'-ATAAC ACTTGAACTGATGTCT                GGATGAGCACT                 TGTCCTAAGTCGAGGT TTTGC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 001-114253666
Forward Primer: 5'-AGGTGTTCTGGGAGGGGAGT-3' T_m: 59.508
Reverse Primer: 5'-GAGTAGATGGTGGTGGTGTGG-3' T_m: 59.378
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 131, 139
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr1:114253774-114253816

Rank: 67	Homology Score: 10
	Genomic Region: Intron	Closest Gene: DSCAML1
Match Type: R23R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-ACGGT GCCATCTGACACCAGT          CCGTGGGTGAGGAGGCGTCTGGG           ACAGGAGTAGGGTGGA TGAAG-3'
	3'-TGCCA CGGTAGACTGTGGTCA          GGCACCCACTCCTCCGCAGACCC           TGTCCTCATCCCACCT ACTTC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 011-117354866
Forward Primer: 5'-GCTCAGGCAAAGTTGTTCCCAG-3' T_m: 59.258
Reverse Primer: 5'-ATGTGCTGAGAGGCTGGTGC-3' T_m: 59.508
Expected PCR Product Length: 286
Expected CelI Cleavage Product Sizes: 130, 156
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr11:117354967-117355021

Rank: 68	Homology Score: 10
	Genomic Region: Intron	Closest Gene: NSMCE2
Match Type: R28R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TGTGC TGCACCTCCCTCGTGT        CATTCTCAAGAATTAAAGTCTTACAGGC        AAAGGACTGAGGTGGC ATATC-3'
	3'-ACACG ACGTGGAGGGAGCACA        GTAAGAGTTCTTAATTTCAGAATGTCCG        TTTCCTGACTCCACCG TATAG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 008-126184331
Forward Primer: 5'-CTGAGTGGTGAAGAAACTTGCCC-3' T_m: 59.148
Reverse Primer: 5'-GCCAAGCTTACCTGTCTAGAACTG-3' T_m: 59.038
Expected PCR Product Length: 310
Expected CelI Cleavage Product Sizes: 150, 160
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr8:126184449-126184508

Rank: 69	Homology Score: 10
	Genomic Region: Intron	Closest Gene: KIRREL3
Match Type: R18R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TCTGT TGCACTTTTCTCTTCT             TAATCTATCGTTTGTTAC             ACGGGTGTCAGCTGCA AACCT-3'
	3'-AGACA ACGTGAAAAGAGAAGA             ATTAGATAGCAAACAATG             TGCCCACAGTCGACGT TTGGA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 011-126353104
Forward Primer: 5'-GAGGTCATACCCCCTTTTTTGTCC-3' T_m: 59.038
Reverse Primer: 5'-GGGCCCAGTTCTGAATCAAACAGC-3' T_m: 60.908
Expected PCR Product Length: 305
Expected CelI Cleavage Product Sizes: 175, 130
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr11:126353252-126353301

Rank: 70	Homology Score: 10
	Genomic Region: Intron	Closest Gene: TEAD1
Match Type: R30R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GTTTC TGCACCTGGCATCTGT       TGATATACACAGGTGGGGGAGGACAGGGGC       ACAGGATACAGTTGGG AGAGA-3'
	3'-CAAAG ACGTGGACCGTAGACA       ACTATATGTGTCCACCCCCTCCTGTCCCCG       TGTCCTATGTCAACCC TCTCT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 011-012784194
Forward Primer: 5'-GCTGCCAAGTCTACAAAGCTGGA-3' T_m: 59.148
Reverse Primer: 5'-ACAACACGGACACCCAGGAG-3' T_m: 59.508
Expected PCR Product Length: 278
Expected CelI Cleavage Product Sizes: 131, 147
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr11:12784292-12784353

Rank: 71	Homology Score: 10
	Genomic Region: Intron	Closest Gene: POTEF
Match Type: R29R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-AGGAT TGCAGCCAGCTCCTGC       CCCGGTGCAGTGTATACAGTGTATACTGC        ACAGGTGTTGGGTGCA CCAAA-3'
	3'-TCCTA ACGTCGGTCGAGGACG       GGGCCACGTCACATATGTCACATATGACG        TGTCCACAACCCACGT GGTTT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Warning! Primer pair was generated using suboptimal parameters. 
Primer Index: 002-130879063
Forward Primer: 5'-AAGACGCCCACCGAGCCCA-3' T_m: 62.008
Reverse Primer: 5'-GCTAATGGGTGCAAAGTTTCTTTTAAGGAGCATA-3' T_m: 59.668
Expected PCR Product Length: 450
Expected CelI Cleavage Product Sizes: 144, 306
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr2:130879175-130879235

Rank: 72	Homology Score: 10
	Genomic Region: Intron	Closest Gene: ACSL6
Match Type: R28R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TCCCA AATACCTGACTCCTGT        TCCAGGTCCTTCCCTGGGCCCTCGGCCC        ACAGAAGTCCTGGACA CTCAA-3'
	3'-AGGGT TTATGGACTGAGGACA        AGGTCCAGGAAGGGACCCGGGAGCCGGG        TGTCTTCAGGACCTGT GAGTT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 005-131322201
Forward Primer: 5'-CTGCATCTTCTGGAGAGTTTGCC-3' T_m: 59.148
Reverse Primer: 5'-TTGAGCGAGATGGCGTGCAG-3' T_m: 59.508
Expected PCR Product Length: 287
Expected CelI Cleavage Product Sizes: 145, 142
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr5:131322314-131322373

Rank: 73	Homology Score: 10
	Genomic Region: Intron	Closest Gene: LOC100507254
Match Type: R28R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-ATCAC TGGAACTGGTTCCAGT        GCGCATATATACTCTGATCAGCTTTTAC        ACAGGAGTCTGTGGCA GAGAA-3'
	3'-TAGTG ACCTTGACCAAGGTCA        CGCGTATATATGAGACTAGTCGAAAATG        TGTCCTCAGACACCGT CTCTT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 006-132465014
Forward Primer: 5'-GTTGTGTTGTGGCTGCTCAGAGT-3' T_m: 59.148
Reverse Primer: 5'-CCTTCTATCCCCATCCTCTCTTTC-3' T_m: 59.038
Expected PCR Product Length: 293
Expected CelI Cleavage Product Sizes: 136, 157
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr6:132465118-132465177

Rank: 74	Homology Score: 10
	Genomic Region: Intron	Closest Gene: JAKMIP3
Match Type: R16R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-AGGGG TGCACCTCACAACTGT              GGGAGCAGGTGATGTG              GCATGAGGCAGGGGCG GGGCT-3'
	3'-TCCCC ACGTGGAGTGTTGACA              CCCTCGTCCACTACAC              CGTACTCCGTCCCCGC CCCGA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 010-133959783
Forward Primer: 5'-TTTCTCTCGGCACCTCCCAC-3' T_m: 59.508
Reverse Primer: 5'-GTCCCTGCCCATGGACAAGT-3' T_m: 59.508
Expected PCR Product Length: 287
Expected CelI Cleavage Product Sizes: 157, 130
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr10:133959914-133959961

Rank: 75	Homology Score: 10
	Genomic Region: Intron	Closest Gene: MED27
Match Type: R27R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TTGTT TACAAATCACTCCTGC        CATCAACCTCATTTTACAGATCAGGAA         ACAGGAGTGAGCTGAA CAACG-3'
	3'-AACAA ATGTTTAGTGAGGACG        GTAGTTGGAGTAAAATGTCTAGTCCTT         TGTCCTCACTCGACTT GTTGC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 009-134936820
Forward Primer: 5'-GCTGGTAAGTGAAGTTGATGTGGC-3' T_m: 59.038
Reverse Primer: 5'-CCAGGAGCAGCTGCGATGAA-3' T_m: 59.508
Expected PCR Product Length: 318
Expected CelI Cleavage Product Sizes: 187, 131
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr9:134936976-134937034

Rank: 76	Homology Score: 10
	Genomic Region: Intron	Closest Gene: SPOCK1
Match Type: R12R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GGAAG TTTGCCAGGCTCCTGT                CCCTCCAGTGTG                ACAGGAGTGAGCTACA ATGCA-3'
	3'-CCTTC AAACGGTCCGAGGACA                GGGAGGTCACAC                TGTCCTCACTCGATGT TACGT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 005-136419378
Forward Primer: 5'-GCTATGGGAGAGCCGAAAACTG-3' T_m: 59.258
Reverse Primer: 5'-TGGAGTGGGCAAGCAAGAGC-3' T_m: 59.508
Expected PCR Product Length: 280
Expected CelI Cleavage Product Sizes: 150, 130
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr5:136419504-136419547

Rank: 77	Homology Score: 10
	Genomic Region: Intron	Closest Gene: SLC6A3
Match Type: R14R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CTCAA TGAATGTGACTCCTGT               GGGAACCTCCCGTC               AGTGGAGTCACGTGGC ACGGG-3'
	3'-GAGTT ACTTACACTGAGGACA               CCCTTGGAGGGCAG               TCACCTCAGTGCACCG TGCCC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 005-001406854
Forward Primer: 5'-TCCATCTTCAGAACTCCATCCTCC-3' T_m: 59.038
Reverse Primer: 5'-CTCATGACGGAATATGATTCGGCC-3' T_m: 59.038
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 136, 134
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr5:1406965-1407010

Rank: 78	Homology Score: 10
	Genomic Region: Intron	Closest Gene: BFAR
Match Type: R26R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GGTGA TCCACCTGCCTCCTGA         CCTCAGCCTCCCAAAGTGCTGGGATT         ACAGGCGTCAGCCACC GCGCC-3'
	3'-CCACT AGGTGGACGGAGGACT         GGAGTCGGAGGGTTTCACGACCCTAA         TGTCCGCAGTCGGTGG CGCGG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 016-014749429
Forward Primer: 5'-TACAGGCACACGCCACCACA-3' T_m: 59.508
Reverse Primer: 5'-CTGGCTTAAAGAGCAGATGCGG-3' T_m: 59.258
Expected PCR Product Length: 293
Expected CelI Cleavage Product Sizes: 131, 162
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr16:14749529-14749586

Rank: 79	Homology Score: 10
	Genomic Region: Intron	Closest Gene: FAM134B
Match Type: R20R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-AAGAT TGCCCCTGACTCAGGT            CCTTGGAAACCCAGAGTTGC            TCAGGATACAGTTGTA AGCAG-3'
	3'-TTCTA ACGGGGACTGAGTCCA            GGAACCTTTGGGTCTCAACG            AGTCCTATGTCAACAT TCGTC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 005-016519335
Forward Primer: 5'-CCTAGATCAGCGGCTTTCAAAGCA-3' T_m: 59.038
Reverse Primer: 5'-GGGCTGAAAGGAGCTTTGAGG-3' T_m: 59.378
Expected PCR Product Length: 374
Expected CelI Cleavage Product Sizes: 189, 185
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr5:16519496-16519547

Rank: 80	Homology Score: 10
	Genomic Region: Intron	Closest Gene: LDB2
Match Type: R18R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CTACG AGCACGTGACTCCTAT             AGGTGCAAGCTTTAGAAG             GTAGGAGTCAAGGCCA GGCAC-3'
	3'-GATGC TCGTGCACTGAGGATA             TCCACGTTCGAAATCTTC             CATCCTCAGTTCCGGT CCGTG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 004-016836013
Forward Primer: 5'-CACCTATGTGGCGTGATTGAGTGA-3' T_m: 59.038
Reverse Primer: 5'-AGACAGGGTTTCAGCATGTTGGC-3' T_m: 59.148
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 139, 131
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr4:16836125-16836174

Rank: 81	Homology Score: 10
	Genomic Region: Intron	Closest Gene: SH3RF1
Match Type: R14R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GTTTC TGTACCTCACTTCTGT               TTTCTGTGAATCAG               AGAGGATTTCGGTCCA TGAAT-3'
	3'-CAAAG ACATGGAGTGAAGACA               AAAGACACTTAGTC               TCTCCTAAAGCCAGGT ACTTA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 004-170092193
Forward Primer: 5'-GACCTAAAGGTTTCTCCACACATAGTGAAG-3' T_m: 60.058
Reverse Primer: 5'-GACTGTTCTGAGATCTTGGGTGTG-3' T_m: 59.038
Expected PCR Product Length: 350
Expected CelI Cleavage Product Sizes: 192, 158
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr4:170092360-170092405

Rank: 82	Homology Score: 10
	Genomic Region: Intron	Closest Gene: PLD1
Match Type: R10R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CTCAG GACACCTGAATCCTGT                 GACGACATTC                 CCAAGGGTCAGAAGCA CATGG-3'
	3'-GAGTC CTGTGGACTTAGGACA                 CTGCTGTAAG                 GGTTCCCAGTCTTCGT GTACC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 003-171470945
Forward Primer: 5'-GACTCTGCTCAGTGGGTGTG-3' T_m: 59.508
Reverse Primer: 5'-AAGCAAGGTGGCTTATCAGTCTGG-3' T_m: 59.038
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 136, 134
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr3:171471058-171471099

Rank: 83	Homology Score: 10
	Genomic Region: Intron	Closest Gene: COL23A1
Match Type: R29R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-ATCCC TGCACCTCACACCTAT       CCTGAAGCAAACTAATCAGTAGTTAAATA        AGAGGAGGAGGGTACA TTACG-3'
	3'-TAGGG ACGTGGAGTGTGGATA       GGACTTCGTTTGATTAGTCATCAATTTAT        TCTCCTCCTCCCATGT AATGC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 005-177943719
Forward Primer: 5'-AAGCTTTCTGCCTCCTCAGGCC-3' T_m: 61.288
Reverse Primer: 5'-CACCTCAGTGACAGGCGCTT-3' T_m: 59.508
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 137, 133
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr5:177943824-177943884

Rank: 84	Homology Score: 10
	Genomic Region: Intron	Closest Gene: GFPT2
Match Type: R19R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GAAGC AGCACCTGCCTGTTCT            TTAACAGGAGTCAGAATCT             CCAGGGGGCAGGTGCA GGAGA-3'
	3'-CTTCG TCGTGGACGGACAAGA            AATTGTCCTCAGTCTTAGA             GGTCCCCCGTCCACGT CCTCT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 005-179732702
Forward Primer: 5'-ATCTCCCGCCAGCACGTTCA-3' T_m: 59.508
Reverse Primer: 5'-GAACGAGACTCCCATCTCAACG-3' T_m: 59.258
Expected PCR Product Length: 318
Expected CelI Cleavage Product Sizes: 138, 180
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr5:179732813-179732863

Rank: 85	Homology Score: 10
	Genomic Region: Intron	Closest Gene: DENND4C
Match Type: R25R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GAGTC TGCAGATGACTTCTGT         TTATTTCCTATAGTACAAAATTAGT          ATGGGAGTGCTGTGCA CAGTG-3'
	3'-CTCAG ACGTCTACTGAAGACA         AATAAAGGATATCATGTTTTAATCA          TACCCTCACGACACGT GTCAC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 009-019336805
Forward Primer: 5'-GTCCATTGATAATCACTCTAGCACAGGTAC-3' T_m: 60.058
Reverse Primer: 5'-GTGTGACTTCCTTCCTTCCCTC-3' T_m: 59.258
Expected PCR Product Length: 422
Expected CelI Cleavage Product Sizes: 199, 223
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr9:19336974-19337030

Rank: 86	Homology Score: 10
	Genomic Region: Intron	Closest Gene: CCDC144C
Match Type: R13R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-AATGG TGCTCCTCATTCCCTT               CTGAGTTCAGAAC                ACAGGAGTCAGATAGA TGGAC-3'
	3'-TTACC ACGAGGAGTAAGGGAA               GACTCAAGTCTTG                TGTCCTCAGTCTATCT ACCTG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 017-020300643
Forward Primer: 5'-GGTGACAGAGCAAGACTCCATC-3' T_m: 59.258
Reverse Primer: 5'-CTCTGTGGGTTAGTGCCTAATCTG-3' T_m: 59.038
Expected PCR Product Length: 312
Expected CelI Cleavage Product Sizes: 181, 131
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr17:20300800-20300844

Rank: 87	Homology Score: 10
	Genomic Region: Intron	Closest Gene: WNT10A
Match Type: R26R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GTGTC TGCCCCAGACAGCTGG         GGCCAGAACCGAAACTGGGGCCCTGA         ACAGGAGCTGGGTGCA GACCC-3'
	3'-CACAG ACGGGGTCTGTCGACC         CCGGTCTTGGCTTTGACCCCGGGACT         TGTCCTCGACCCACGT CTGGG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 002-219751327
Forward Primer: 5'-TGTGTCTTAACGAGCCGCCC-3' T_m: 59.508
Reverse Primer: 5'-CTGACAGCTGTCATAGCCACG-3' T_m: 59.378
Expected PCR Product Length: 284
Expected CelI Cleavage Product Sizes: 154, 130
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr2:219751450-219751507

Rank: 88	Homology Score: 10
	Genomic Region: Intron	Closest Gene: ZNF676
Match Type: R15R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CCTAC TGCCGCTGTCTCCTTC              TTATTTTGTCAGACA               ACAGGAGTCAGGTTTT GAGTC-3'
	3'-GGATG ACGGCGACAGAGGAAG              AATAAAACAGTCTGT               TGTCCTCAGTCCAAAA CTCAG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 019-022376173
Forward Primer: 5'-GGTACTCTCACTGTGGAAAACTGG-3' T_m: 59.038
Reverse Primer: 5'-GGCCAATTCTCCTCTCCTTTTTCC-3' T_m: 59.038
Expected PCR Product Length: 287
Expected CelI Cleavage Product Sizes: 146, 141
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr19:22376294-22376340

Rank: 89	Homology Score: 10
	Genomic Region: Intron	Closest Gene: SGSM2
Match Type: R10R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CCTGG TGCTTCTGACTCCTGA                 CTTAACAGAA                 ACAGGATGGAGGTACC GGGTC-3'
	3'-GGACC ACGAAGACTGAGGACT                 GAATTGTCTT                 TGTCCTACCTCCATGG CCCAG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 017-002270979
Forward Primer: 5'-GAATGGATCTCTGAGCCCATTCCT-3' T_m: 59.038
Reverse Primer: 5'-CATGCCAAAGTACCAGGCCC-3' T_m: 59.508
Expected PCR Product Length: 284
Expected CelI Cleavage Product Sizes: 131, 153
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr17:2271087-2271128

Rank: 90	Homology Score: 10
	Genomic Region: Intron	Closest Gene: CDC42BPA
Match Type: R26R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-ACTTC TGCAGCTGACTCAACA         ATCTGGGTTTCAACCACCCTTCCTCC         ATAGGATTCAGATGCA TGCCA-3'
	3'-TGAAG ACGTCGACTGAGTTGT         TAGACCCAAAGTTGGTGGGAAGGAGG         TATCCTAAGTCTACGT ACGGT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 001-227379954
Forward Primer: 5'-GAGATCACTCTCACCACCCAAAAC-3' T_m: 59.038
Reverse Primer: 5'-GGAAACAGCAGAACGTTGTTGGTG-3' T_m: 59.038
Expected PCR Product Length: 383
Expected CelI Cleavage Product Sizes: 190, 193
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr1:227380113-227380170

Rank: 91	Homology Score: 10
	Genomic Region: Intron	Closest Gene: C1orf198
Match Type: R20R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GGGCT TGCCCTTGACACCTGT            CTCAGGCCCAGTTTGGCTAC            ATGTGAGACTGGTGCA GAGTG-3'
	3'-CCCGA ACGGGAACTGTGGACA            GAGTCCGGGTCAAACCGATG            TACACTCTGACCACGT CTCAC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 001-230976433
Forward Primer: 5'-GCGCCAAGGTCTTCTTGAGC-3' T_m: 59.508
Reverse Primer: 5'-GGAGAGTAGGAGACAGCACATG-3' T_m: 59.258
Expected PCR Product Length: 312
Expected CelI Cleavage Product Sizes: 136, 176
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr1:230976541-230976592

Rank: 92	Homology Score: 10
	Genomic Region: Intron	Closest Gene: KLHL29
Match Type: R14R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TCCTG TGCACATGAGGCCTGT               GGCCATGTCAGGCC               CTGGGAGAGAGGTGCA AGTCT-3'
	3'-AGGAC ACGTGTACTCCGGACA               CCGGTACAGTCCGG               GACCCTCTCTCCACGT TCAGA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 002-023872858
Forward Primer: 5'-GTGGTCTTAGGCACCTCCTG-3' T_m: 59.508
Reverse Primer: 5'-CTCACTGTCCCCTTAACTTTCCTG-3' T_m: 59.038
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 136, 134
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr2:23872969-23873014

Rank: 93	Homology Score: 10
	Genomic Region: Intron	Closest Gene: APP
Match Type: R30R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CTCTT TGCAGCTGACCCCTTT       AACGTTACCCTCTAAAACATCTACTTCTTT       ACTGGAATTAGCTACA AGCAC-3'
	3'-GAGAA ACGTCGACTGGGGAAA       TTGCAATGGGAGATTTTGTAGATGAAGAAA       TGACCTTAATCGATGT TCGTG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 021-027390640
Forward Primer: 5'-CCTCCCTGCAGTATCTCATCTTTG-3' T_m: 59.038
Reverse Primer: 5'-GCAGGTCCATTCATTCCACCTC-3' T_m: 59.258
Expected PCR Product Length: 372
Expected CelI Cleavage Product Sizes: 214, 158
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr21:27390821-27390882

Rank: 94	Homology Score: 10
	Genomic Region: Intron	Closest Gene: BDNF-AS
Match Type: R12R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TCTGA TACCTCTGACTCCTGT                TTACCTAGAGGA                AGAAGAATCTGGAGCA GCAGT-3'
	3'-AGACT ATGGAGACTGAGGACA                AATGGATCTCCT                TCTTCTTAGACCTCGT CGTCA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 011-027668392
Forward Primer: 5'-GGTGGGTCCATTCTGACTCTG-3' T_m: 59.378
Reverse Primer: 5'-TCATGATCCACCCACCTCGG-3' T_m: 59.508
Expected PCR Product Length: 362
Expected CelI Cleavage Product Sizes: 219, 143
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr11:27668587-27668630

Rank: 95	Homology Score: 10
	Genomic Region: Intron	Closest Gene: LOC100507605
Match Type: R27R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TCTGG CGCAACTCACCCCTGG        CTTGCACCAAACATTCTTAAAATGTTT         ATTGGGGTCAGGTGCA ATGGC-3'
	3'-AGACC GCGTTGAGTGGGGACC        GAACGTGGTTTGTAAGAATTTTACAAA         TAACCCCAGTCCACGT TACCG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 010-029082810
Forward Primer: 5'-CCTCTGAGCCCCATTCTCCT-3' T_m: 59.508
Reverse Primer: 5'-TAGAGATGGGGTCTCACCTTGTTG-3' T_m: 59.038
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 135, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr10:29082914-29082972

Rank: 96	Homology Score: 10
	Genomic Region: Intron	Closest Gene: TGFBR2
Match Type: R17R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-ATTCC TCCACCTGAGTCCTAT             TGAGGTGCTCCCCATTA              TAAGGAGTCCTGTGAA TCAAA-3'
	3'-TAAGG AGGTGGACTCAGGATA             ACTCCACGAGGGGTAAT              ATTCCTCAGGACACTT AGTTT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 003-030699112
Forward Primer: 5'-CTGGCACAGAGTGAGCACTTAATG-3' T_m: 59.038
Reverse Primer: 5'-GGTCGTCATGAGGAGAACACCTT-3' T_m: 59.148
Expected PCR Product Length: 327
Expected CelI Cleavage Product Sizes: 172, 155
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr3:30699258-30699306

Rank: 97	Homology Score: 10
	Genomic Region: Intron	Closest Gene: LOC100507194
Match Type: R30R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-AGATT TGCATGTGAAGCATGT       CAGAGAATGACGAATGGAGGACTGGGGATG       CCAGGAGTCAGGTGGT GCATT-3'
	3'-TCTAA ACGTACACTTCGTACA       GTCTCTTACTGCTTACCTCCTGACCCCTAC       GGTCCTCAGTCCACCA CGTAA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 006-003193167
Forward Primer: 5'-CACCACAACCGGCCGAGAAT-3' T_m: 59.508
Reverse Primer: 5'-CCCTGGGGGATAGTACAAAGGT-3' T_m: 59.258
Expected PCR Product Length: 374
Expected CelI Cleavage Product Sizes: 233, 141
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr6:3193367-3193428

Rank: 98	Homology Score: 10
	Genomic Region: Intron	Closest Gene: CCNY
Match Type: R30R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GAAAA TGAACCTTACTCTTGT       ATCCAGGATATTTTGTAAGGGAGCATTGTA       ACAGGAATCCAGTGAC CCTTG-3'
	3'-CTTTT ACTTGGAATGAGAACA       TAGGTCCTATAAAACATTCCCTCGTAACAT       TGTCCTTAGGTCACTG GGAAC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 010-035637732
Forward Primer: 5'-GGGATGGTCTGAATCTGGACAC-3' T_m: 59.258
Reverse Primer: 5'-GCGGCCCTCTATTTTACATCCC-3' T_m: 59.258
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 133, 137
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr10:35637832-35637893

Rank: 99	Homology Score: 10
	Genomic Region: Intron	Closest Gene: CSMD1
Match Type: R27R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GGGAA AGGACCTGAATCCTGT        GCTGAGGGTGCTGCCCCTAAGGTGGGC         ACAGGAGGAAGCACCA CCTGG-3'
	3'-CCCTT TCCTGGACTTAGGACA        CGACTCCCACGACGGGGATTCCACCCG         TGTCCTCCTTCGTGGT GGACC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 008-003640891
Forward Primer: 5'-AAGCAGTCACATGCCCCTGG-3' T_m: 59.508
Reverse Primer: 5'-CAGCAGGGTGCTTCAACCATG-3' T_m: 59.378
Expected PCR Product Length: 303
Expected CelI Cleavage Product Sizes: 167, 136
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr8:3641027-3641085

Rank: 100	Homology Score: 10
	Genomic Region: Intron	Closest Gene: ZZEF1
Match Type: R29R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-ACTAC TGCCACAGACTCCTAA       CTGCTTTTCTTATTTCCACCTCTTTTCAC        ACAGCAGCCAGGGGCA CCTCT-3'
	3'-TGATG ACGGTGTCTGAGGATT       GACGAAAAGAATAAAGGTGGAGAAAAGTG        TGTCGTCGGTCCCCGT GGAGA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 017-003965044
Forward Primer: 5'-CGTAAGTCCTGACAACTCTACCTC-3' T_m: 59.038
Reverse Primer: 5'-GAAAGGCAGGACCCTTATCACTGT-3' T_m: 59.038
Expected PCR Product Length: 290
Expected CelI Cleavage Product Sizes: 143, 147
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr17:3965155-3965215

Rank: 101	Homology Score: 10
	Genomic Region: Intron	Closest Gene: PLCG1
Match Type: R20R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-AGCTT CTCTCCAGACTCTTGT            GTGTGTGTGTACACAGATGC            ACAGGATTCAGTTGGA GTTTA-3'
	3'-TCGAA GAGAGGTCTGAGAACA            CACACACACATGTGTCTACG            TGTCCTAAGTCAACCT CAAAT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 020-039770399
Forward Primer: 5'-CCAGCTGGGATCCTCTGTTTC-3' T_m: 59.378
Reverse Primer: 5'-CCTAGCACTGGGCAAATGGG-3' T_m: 59.508
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 135, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr20:39770506-39770557

Rank: 102	Homology Score: 10
	Genomic Region: Intron	Closest Gene: SLC2A13
Match Type: R19R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TAAGA TTCAACTGACTCCTGA            GTTAGATGCAAAAAATTTT             ATAGTAGTGAGGTCTA CTGTT-3'
	3'-ATTCT AAGTTGACTGAGGACT            CAATCTACGTTTTTTAAAA             TATCATCACTCCAGAT GACAA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 012-040234817
Forward Primer: 5'-CCTTACAATTCACGGCTCTCAGAC-3' T_m: 59.038
Reverse Primer: 5'-CCACTGGAGTTGACAGATTTGGC-3' T_m: 59.148
Expected PCR Product Length: 436
Expected CelI Cleavage Product Sizes: 130, 306
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr12:40234920-40234970

Rank: 103	Homology Score: 10
	Genomic Region: Intron	Closest Gene: FBL
Match Type: R12R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CCCAC GGGGACTGAATCCTGT                GGGGGAAACAAA                ACAGGAGTCAGGGCAA TGAAG-3'
	3'-GGGTG CCCCTGACTTAGGACA                CCCCCTTTGTTT                TGTCCTCAGTCCCGTT ACTTC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 019-040331309
Forward Primer: 5'-ACGACCTCTAAAGCCTCCGC-3' T_m: 59.508
Reverse Primer: 5'-GCACAGCAAGACCCTGTCTC-3' T_m: 59.508
Expected PCR Product Length: 441
Expected CelI Cleavage Product Sizes: 131, 310
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr19:40331416-40331459

Rank: 104	Homology Score: 10
	Genomic Region: Intron	Closest Gene: VPS18
Match Type: R14R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TTTGC ACCACTGTACTCCTGT               CTCTTTAAAAAAAA               AAAAAAGTCAGGTGCA GTGGC-3'
	3'-AAACG TGGTGACATGAGGACA               GAGAAATTTTTTTT               TTTTTTCAGTCCACGT CACCG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 015-041188560
Forward Primer: 5'-GACCCCCGTCTCTACCAAAAC-3' T_m: 59.378
Reverse Primer: 5'-CCACGCCCGGCTAACTTTTG-3' T_m: 59.508
Expected PCR Product Length: 314
Expected CelI Cleavage Product Sizes: 157, 157
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr15:41188692-41188737

Rank: 105	Homology Score: 10
	Genomic Region: Intron	Closest Gene: ANK1
Match Type: R15R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TATAA TGAACCTGGCTCCCGT              TCCTTTTATATAAGT               AGGGGAGTGAGGAGGA GGGGA-3'
	3'-ATATT ACTTGGACCGAGGGCA              AGGAAAATATATTCA               TCCCCTCACTCCTCCT CCCCT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 008-041570215
Forward Primer: 5'-GGGTGCAGAGAGATTTGCATGTG-3' T_m: 59.148
Reverse Primer: 5'-TGTCTCCTCCTGTCCCTGCT-3' T_m: 59.508
Expected PCR Product Length: 289
Expected CelI Cleavage Product Sizes: 153, 136
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr8:41570343-41570389

Rank: 106	Homology Score: 10
	Genomic Region: Intron	Closest Gene: ST3GAL3
Match Type: R24R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GGGCA GCCTCCTGGCTCCTGA          GGTTCCATCTATGCTGTGAGAGAC          ACAGGGGTCAGATCCA CACTC-3'
	3'-CCCGT CGGAGGACCGAGGACT          CCAAGGTAGATACGACACTCTCTG          TGTCCCCAGTCTAGGT GTGAG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 001-044350778
Forward Primer: 5'-TGGAAGATCTCTTAGGAGGGCC-3' T_m: 59.258
Reverse Primer: 5'-GGGAAGGAGGAGACGGAAGT-3' T_m: 59.508
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 136, 134
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr1:44350884-44350939

Rank: 107	Homology Score: 10
	Genomic Region: Intron	Closest Gene: TMEM72-AS1
Match Type: R29R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GGGGG TGCCCATGACTCCCCC       TTAGGTTTAACAATGCACTTGAATGACTC        ACAGGACTCAGGAGGA CACTC-3'
	3'-CCCCC ACGGGTACTGAGGGGG       AATCCAAATTGTTACGTGAACTTACTGAG        TGTCCTGAGTCCTCCT GTGAG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 010-045378787
Forward Primer: 5'-GGTGAGGCTCCATCCCACAA-3' T_m: 59.508
Reverse Primer: 5'-ACCCAGAGAGGGTGTGAGAG-3' T_m: 59.508
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 133, 137
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr10:45378888-45378948

Rank: 108	Homology Score: 10
	Genomic Region: Intron	Closest Gene: EIF2B3
Match Type: R12R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-AAAAA TGCAGCTCACACCTGT                GATCCCAGTACT                TTAGGAGGCAGAGGCA GGCAG-3'
	3'-TTTTT ACGTCGAGTGTGGACA                CTAGGGTCATGA                AATCCTCCGTCTCCGT CCGTC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 001-045422945
Forward Primer: 5'-GCCCCCAAACACCACAAAGTC-3' T_m: 59.378
Reverse Primer: 5'-ATTACAGGCACATGCCACCACG-3' T_m: 59.258
Expected PCR Product Length: 370
Expected CelI Cleavage Product Sizes: 240, 130
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr1:45423161-45423204

Rank: 109	Homology Score: 10
	Genomic Region: Intron	Closest Gene: HMGN2P46
Match Type: R18R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GCCCA TGCACTTGACTTCTGC             TAGTCCCTTGAAGGCTGT             ACCAGAGTCAAGGGCC ATCTT-3'
	3'-CGGGT ACGTGAACTGAAGACG             ATCAGGGAACTTCCGACA             TGGTCTCAGTTCCCGG TAGAA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Warning! Primer pair was generated using suboptimal parameters. 
Primer Index: 015-045847122
Forward Primer: 5'-CTCTCAGCATGTTTTTGAGATTTATCCATTTATGGTG-3' T_m: 59.428
Reverse Primer: 5'-CAGATGCAAGAAGATCTAAAGCTGGAATAAATTAGATG-3' T_m: 59.358
Expected PCR Product Length: 412
Expected CelI Cleavage Product Sizes: 279, 133
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr15:45847374-45847423

Rank: 110	Homology Score: 10
	Genomic Region: Intron	Closest Gene: SYMPK
Match Type: R23R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CCACC TGCTGCTGCTTCCTGA          GCCTGCTCTGCGGTGAATGCCAA           ACAGGAGCCAGGGCCA GGCTC-3'
	3'-GGTGG ACGACGACGAAGGACT          CGGACGAGACGCCACTTACGGTT           TGTCCTCGGTCCCGGT CCGAG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 019-046327426
Forward Primer: 5'-GTGCTTCGTTCACTGGTGAAGATG-3' T_m: 59.038
Reverse Primer: 5'-GTCCAGCTGTGGAAAAGTGATCTC-3' T_m: 59.038
Expected PCR Product Length: 284
Expected CelI Cleavage Product Sizes: 134, 150
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr19:46327531-46327585

Rank: 111	Homology Score: 10
	Genomic Region: Intron	Closest Gene: SLC9A8
Match Type: R27R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CCACA TGCACCTTCCTTCTGT        TCCTCAGGCAGCAGGCTTGCTCCCACC         GCAGGAGTCGCTTGCT GTCAC-3'
	3'-GGTGT ACGTGGAAGGAAGACA        AGGAGTCCGTCGTCCGAACGAGGGTGG         CGTCCTCAGCGAACGA CAGTG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 020-048470754
Forward Primer: 5'-GAGAGTGAATTGTCCAGGCTCATG-3' T_m: 59.038
Reverse Primer: 5'-TTAGATGGGGCGGTCTGGAG-3' T_m: 59.508
Expected PCR Product Length: 280
Expected CelI Cleavage Product Sizes: 144, 136
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr20:48470867-48470925

Rank: 112	Homology Score: 10
	Genomic Region: Intron	Closest Gene: KRT82
Match Type: R23R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CCCCA TGCACCTCTCTCCTTT          CCCTAGAGCCTGGGCAAGGCCAG           GCTGGATTCAGCTTCA CCATT-3'
	3'-GGGGT ACGTGGAGAGAGGAAA          GGGATCTCGGACCCGTTCCGGTC           CGACCTAAGTCGAAGT GGTAA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 012-052789060
Forward Primer: 5'-ATTCCTCCCACCTCCTGCCA-3' T_m: 59.508
Reverse Primer: 5'-TTCAGGGCCCAGAAGCCACA-3' T_m: 59.508
Expected PCR Product Length: 267
Expected CelI Cleavage Product Sizes: 131, 136
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr12:52789162-52789216

Rank: 113	Homology Score: 10
	Genomic Region: Intron	Closest Gene: LNX1
Match Type: R10R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-ATACC TGCACCTGCCTCTTGC                 CCTTGTCATG                 ACAGGAACCAGGATAA ACAAC-3'
	3'-TATGG ACGTGGACGGAGAACG                 GGAACAGTAC                 TGTCCTTGGTCCTATT TGTTG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 004-054438610
Forward Primer: 5'-CCCAGGCCATTGCTTTTGATGC-3' T_m: 59.258
Reverse Primer: 5'-GGCTGCCTCTCATTTTGTCCAC-3' T_m: 59.258
Expected PCR Product Length: 279
Expected CelI Cleavage Product Sizes: 134, 145
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr4:54438721-54438762

Rank: 114	Homology Score: 10
	Genomic Region: Intron	Closest Gene: TOX
Match Type: R22R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-AAAGG TCACACTGACTCTTGT           GTGGAGAATGTATCATGGAGGA           ACAGGAGTCAGGAGGC GAGGC-3'
	3'-TTTCC AGTGTGACTGAGAACA           CACCTCTTACATAGTACCTCCT           TGTCCTCAGTCCTCCG CTCCG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 008-059835610
Forward Primer: 5'-GGGATCTTGGCAGAGCCTGA-3' T_m: 59.508
Reverse Primer: 5'-CAAGTCTCCACAGAAATGCCTCC-3' T_m: 59.148
Expected PCR Product Length: 303
Expected CelI Cleavage Product Sizes: 148, 155
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr8:59835729-59835782

Rank: 115	Homology Score: 10
	Genomic Region: Intron	Closest Gene: CDH4
Match Type: R13R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TGCAT TTCTCCTGACTTGTAT               TACTCATTTTACG                GCTGGAGACAGGTGCA CATGG-3'
	3'-ACGTA AAGAGGACTGAACATA               ATGAGTAAAATGC                CGACCTCTGTCCACGT GTACC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 020-059992627
Forward Primer: 5'-TGGCCCTGTTTCTGGCTGGA-3' T_m: 59.508
Reverse Primer: 5'-AGTACACTGAAGTGCAGTCTCTGG-3' T_m: 59.038
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 136, 134
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr20:59992739-59992783

Rank: 116	Homology Score: 10
	Genomic Region: Intron	Closest Gene: MS4A4A
Match Type: R22R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TGACC AGAACCTGACCTATGT           TACCAAAGAATTATGGGAAAGC           ATAGGAGTCAGTTACA AGCTT-3'
	3'-ACTGG TCTTGGACTGGATACA           ATGGTTTCTTAATACCCTTTCG           TATCCTCAGTCAATGT TCGAA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 011-060049460
Forward Primer: 5'-GGGGACTTCATGTGCCGTAG-3' T_m: 59.508
Reverse Primer: 5'-GGTCTGTCTTATCAAACGCACTGG-3' T_m: 59.038
Expected PCR Product Length: 281
Expected CelI Cleavage Product Sizes: 150, 131
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr11:60049581-60049634

Rank: 117	Homology Score: 10
	Genomic Region: Intron	Closest Gene: CDH4
Match Type: R23R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TCTGA TGCCCCTGACTCCTCC          CGGCTCCCTCCCCCAGGACTACG           ACCTCAGCCAGCTGCA GCAGC-3'
	3'-AGACT ACGGGGACTGAGGAGG          GCCGAGGGAGGGGGTCCTGATGC           TGGAGTCGGTCGACGT CGTCG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 020-060508972
Forward Primer: 5'-CATGGTTCTGTGGGATGCTTGG-3' T_m: 59.258
Reverse Primer: 5'-ACCATGGGCCTGATCGGGTA-3' T_m: 59.508
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 139, 131
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr20:60509082-60509136

Rank: 118	Homology Score: 10
	Genomic Region: Intron	Closest Gene: PFKFB3
Match Type: R20R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TCCTG TGCACCTGTCTCATTT            CCTCAACAAGGAATAGAATG            CTTGCAGTCAGGTGCC CCAGC-3'
	3'-AGGAC ACGTGGACAGAGTAAA            GGAGTTGTTCCTTATCTTAC            GAACGTCAGTCCACGG GGTCG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 010-006225239
Forward Primer: 5'-CGCCAACTTTGGGGTTGGCT-3' T_m: 59.508
Reverse Primer: 5'-AAGTGCTGGGATTACAGGCATGAG-3' T_m: 59.038
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 138, 132
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr10:6225349-6225400

Rank: 119	Homology Score: 10
	Genomic Region: Intron	Closest Gene: PCDH9
Match Type: R17R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-ATGCT TGCACCACAGTTCTTT             GTGAATGGGGGATGTGG              ACTGGATTCAGGTGAA AGAAA-3'
	3'-TACGA ACGTGGTGTCAAGAAA             CACTTACCCCCTACACC              TGACCTAAGTCCACTT TCTTT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 013-067512369
Forward Primer: 5'-GGCATCAACAGGGCTTCTTAAACG-3' T_m: 59.038
Reverse Primer: 5'-CAATGTCAGTCGTTTCTGCATGAAGATAGG-3' T_m: 60.058
Expected PCR Product Length: 388
Expected CelI Cleavage Product Sizes: 227, 161
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr13:67512570-67512618

Rank: 120	Homology Score: 10
	Genomic Region: Intron	Closest Gene: PRMT7
Match Type: R22R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GGAAA TGCACGTGGCTCCTGC           TTGTCATCCCAGCACTCTGGGA           ACCTGAGGCTGGTGGA TTGCT-3'
	3'-CCTTT ACGTGCACCGAGGACG           AACAGTAGGGTCGTGAGACCCT           TGGACTCCGACCACCT AACGA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 016-068387900
Forward Primer: 5'-GCGCCAGTGGTCCTTGGATT-3' T_m: 59.508
Reverse Primer: 5'-CTGGGAATACAGGTGTGCACTAC-3' T_m: 59.148
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 134, 136
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr16:68388005-68388058

Rank: 121	Homology Score: 10
	Genomic Region: Intron	Closest Gene: GOLGA6B
Match Type: R15R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CTGCC TGCACCTGGCTCATCT              GTTGGCCTTGGCCTG               GAAGGAGCCAGAGGCA GAGGC-3'
	3'-GACGG ACGTGGACCGAGTAGA              CAACCGGAACCGGAC               CTTCCTCGGTCTCCGT CTCCG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 015-072957610
Forward Primer: 5'-TACTCACCTCTTGCCTTCAAGTGG-3' T_m: 59.038
Reverse Primer: 5'-CTTTTCTTGGCCCATGCCAGG-3' T_m: 59.378
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 133, 137
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr15:72957718-72957764

Rank: 122	Homology Score: 10
	Genomic Region: Intron	Closest Gene: MLXIPL
Match Type: R17R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CACCC TTCACCCCATCCCTGT             CTGCTCAGCGCAAGGCT              ACAGGAGGCAGGGGCT GGTCT-3'
	3'-GTGGG AAGTGGGGTAGGGACA             GACGAGTCGCGTTCCGA              TGTCCTCCGTCCCCGA CCAGA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 007-073021712
Forward Primer: 5'-GATGCGCCGCTTCCAGTAGT-3' T_m: 59.508
Reverse Primer: 5'-TGTGAAGCGGAGGAAGAGCC-3' T_m: 59.508
Expected PCR Product Length: 290
Expected CelI Cleavage Product Sizes: 130, 160
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr7:73021816-73021864

Rank: 123	Homology Score: 10
	Genomic Region: Intron	Closest Gene: PIBF1
Match Type: R23R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-AAATT TACCCCTCTCTCCTCT          GAATTTAGAACTATTAAGGAGAA           ACAAGAGTCAGTTACA TATCT-3'
	3'-TTTAA ATGGGGAGAGAGGAGA          CTTAAATCTTGATAATTCCTCTT           TGTTCTCAGTCAATGT ATAGA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 013-073489472
Forward Primer: 5'-GCGCTCTGCTTCTAAGACACATTG-3' T_m: 59.038
Reverse Primer: 5'-CACATGGAAGAGATGATACATGCCATTACC-3' T_m: 60.058
Expected PCR Product Length: 386
Expected CelI Cleavage Product Sizes: 243, 143
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr13:73489686-73489740

Rank: 124	Homology Score: 10
	Genomic Region: Intron	Closest Gene: SLC16A2
Match Type: R21R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GCATG TTTACCTGATCCCTGA           TGTTCCAATCTCATAGTGACC            ACAGGAGCCTGGAGCA TAGTA-3'
	3'-CGTAC AAATGGACTAGGGACT           ACAAGGTTAGAGTATCACTGG            TGTCCTCGGACCTCGT ATCAT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 00X-073723246
Forward Primer: 5'-GTACAGTGGGGAGTCTGGAG-3' T_m: 59.508
Reverse Primer: 5'-CAGAAGACCAGAAGTAGGCCTAAC-3' T_m: 59.038
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 135, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chrX:73723353-73723405

Rank: 125	Homology Score: 10
	Genomic Region: Intron	Closest Gene: KANK1
Match Type: R27R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GTTAG TGCGCCTGGTTCCTGT        GCTCAGGAATGCACCCGTAACCAGCAG         ACAGGACTGCGGTGGC CATTC-3'
	3'-CAATC ACGCGGACCAAGGACA        CGAGTCCTTACGTGGGCATTGGTCGTC         TGTCCTGACGCCACCG GTAAG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 009-000740828
Forward Primer: 5'-ACACCCCCATCATGTTGGCG-3' T_m: 59.508
Reverse Primer: 5'-TTTGGTCAGCAAGGAGTGCCAG-3' T_m: 59.258
Expected PCR Product Length: 274
Expected CelI Cleavage Product Sizes: 143, 131
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr9:740940-740998

Rank: 126	Homology Score: 10
	Genomic Region: Intron	Closest Gene: GOLGA6A
Match Type: R15R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GCCTC TGCCTCTGGCTCCTTC              CAGGCGAAGGCCAAC               AGATGAGCCAGGTGCA GGCAG-3'
	3'-CGGAG ACGGAGACCGAGGAAG              GTCCGCTTCCGGTTG               TCTACTCGGTCCACGT CCGTC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 015-074364061
Forward Primer: 5'-TTGGCCCATGCCAGGACTCA-3' T_m: 59.508
Reverse Primer: 5'-GCCCCTACAAGAATACTCACCTC-3' T_m: 59.148
Expected PCR Product Length: 277
Expected CelI Cleavage Product Sizes: 134, 143
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr15:74364170-74364216

Rank: 127	Homology Score: 10
	Genomic Region: Intron	Closest Gene: MBP
Match Type: R20R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GGCTC TGCTCCTCTCTCCTGT            CTTGGCTGGCAGGGGCTGCC            CCTGGGGTCGGGTGGA CTCCA-3'
	3'-CCGAG ACGAGGAGAGAGGACA            GAACCGACCGTCCCCGACGG            GGACCCCAGCCCACCT GAGGT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 018-074790610
Forward Primer: 5'-GAGCTGTGGACTGAAGAGCAAC-3' T_m: 59.258
Reverse Primer: 5'-CACAGTCTTGGGAGGGTGCT-3' T_m: 59.508
Expected PCR Product Length: 304
Expected CelI Cleavage Product Sizes: 133, 171
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr18:74790715-74790766

Rank: 128	Homology Score: 10
	Genomic Region: Intron	Closest Gene: SEPT9
Match Type: R11R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CCCCA GGCCTCTGACCCGTGT                GTGGTGTCTTA                 ACAGCAGCCAGGAGCA GGGCC-3'
	3'-GGGGT CCGGAGACTGGGCACA                CACCACAGAAT                 TGTCGTCGGTCCTCGT CCCGG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 017-075477142
Forward Primer: 5'-TGTACCAGCCAGCCCTGATG-3' T_m: 59.508
Reverse Primer: 5'-TGCACACCCACAGGCCAAGA-3' T_m: 59.508
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 138, 132
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr17:75477257-75477299

Rank: 129	Homology Score: 10
	Genomic Region: Intron	Closest Gene: GOLGA6C
Match Type: R15R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CCGCC TGCACCTGGCTCATCT              GGTGGCCTTGGCCTG               GAAGGAGCCAGAGGCA GAGGC-3'
	3'-GGCGG ACGTGGACCGAGTAGA              CCACCGGAACCGGAC               CTTCCTCGGTCTCCGT CTCCG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 015-075561464
Forward Primer: 5'-TACTCACCTCTTGCCTTCAAGTGG-3' T_m: 59.038
Reverse Primer: 5'-TTCTTGGCCCATGCCAGGAC-3' T_m: 59.508
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 133, 137
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr15:75561572-75561618

Rank: 130	Homology Score: 10
	Genomic Region: Intron	Closest Gene: GOLGA6D
Match Type: R15R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CTGCC TGCACCTGGCTCATCT              GGTGGCCTTGGCCTG               GAAGGAGCCAGAGGCA GAGGC-3'
	3'-GACGG ACGTGGACCGAGTAGA              CCACCGGAACCGGAC               CTTCCTCGGTCTCCGT CTCCG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 015-075585740
Forward Primer: 5'-TACTCACCTCTTGCCTTCAAGTGG-3' T_m: 59.038
Reverse Primer: 5'-TTCTTGGCCCATGCCAGGAC-3' T_m: 59.508
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 133, 137
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr15:75585848-75585894

Rank: 131	Homology Score: 10
	Genomic Region: Intron	Closest Gene: IQGAP2
Match Type: R29R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TGGTC TGAAAGAGACTCGTGT       GAAGGTCTAGAACAGGAAGGGGGAAGTAA        ACGGGTGTGAGGTGCA GCCAG-3'
	3'-ACCAG ACTTTCTCTGAGCACA       CTTCCAGATCTTGTCCTTCCCCCTTCATT        TGCCCACACTCCACGT CGGTC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 005-075922611
Forward Primer: 5'-AGGGTAGCCCGGGAAGACTTT-3' T_m: 59.378
Reverse Primer: 5'-TAAAGCTTCCCCAAAGGCTTCCC-3' T_m: 59.148
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 135, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr5:75922714-75922774

Rank: 132	Homology Score: 10
	Genomic Region: Intron	Closest Gene: RBFOX1
Match Type: R12R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GTGGT GGCACCAGACACCTGT                AATCTCAAGTAC                TCAGGAGGCTGAGGCA GGGGA-3'
	3'-CACCA CCGTGGTCTGTGGACA                TTAGAGTTCATG                AGTCCTCCGACTCCGT CCCCT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 016-007614298
Forward Primer: 5'-TTGGGACGCAGAGGTGGGTA-3' T_m: 59.508
Reverse Primer: 5'-CTGGCTTGCAGATGAGTGCCT-3' T_m: 59.378
Expected PCR Product Length: 293
Expected CelI Cleavage Product Sizes: 132, 161
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr16:7614406-7614449

Rank: 133	Homology Score: 10
	Genomic Region: Intron	Closest Gene: C14orf166B
Match Type: R24R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TAACT TGCCCCCAACTCATCT          TTTCTTAGATTAGCTCTGGCAATC          ACAGGAGGCAGATGTA ACAGT-3'
	3'-ATTGA ACGGGGGTTGAGTAGA          AAAGAATCTAATCGAGACCGTTAG          TGTCCTCCGTCTACAT TGTCA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 014-077327823
Forward Primer: 5'-CCAACAGCTGGTAAGGATGTCAAG-3' T_m: 59.038
Reverse Primer: 5'-CTGTGGAAACACCAAAGCAGGG-3' T_m: 59.258
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 133, 137
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr14:77327926-77327981

Rank: 134	Homology Score: 10
	Genomic Region: Intron	Closest Gene: CTSH
Match Type: R17R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CGCCC AGCACCTGGCAGGTGT             CATGAAGGGTCTGACAC              ACAGGAGTCTGGGCCA CGAGC-3'
	3'-GCGGG TCGTGGACCGTCCACA             GTACTTCCCAGACTGTG              TGTCCTCAGACCCGGT GCTCG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 015-079217430
Forward Primer: 5'-CCTGTGTCTGTCGTATCCCTTC-3' T_m: 59.258
Reverse Primer: 5'-GTATAGAACCGGCATCTACTCCAG-3' T_m: 59.038
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 137, 133
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr15:79217541-79217589

Rank: 135	Homology Score: 10
	Genomic Region: Intron	Closest Gene: TMED3
Match Type: R17R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TCCTG TATTCCTGTCTCCTTT             GAGAGATCCCGTGGCCC              ACAGGTGACAGGAGCA GGTCT-3'
	3'-AGGAC ATAAGGACAGAGGAAA             CTCTCTAGGGCACCGGG              TGTCCACTGTCCTCGT CCAGA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 015-079604259
Forward Primer: 5'-TTGGAAAGGAGGGATCAGTGCAG-3' T_m: 59.148
Reverse Primer: 5'-GTGCCTATCAGCATGCGTCC-3' T_m: 59.508
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 135, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr15:79604368-79604416

Rank: 136	Homology Score: 10
	Genomic Region: Intron	Closest Gene: ICA1
Match Type: R26R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GGGTT TTCTCCTGGCTCCACT         CCAGTTTCCTCCCATATCCCAAAGCT         ACACAAGTCAGGTGAA TGTGC-3'
	3'-CCCAA AAGAGGACCGAGGTGA         GGTCAAAGGAGGGTATAGGGTTTCGA         TGTGTTCAGTCCACTT ACACG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 007-008187341
Forward Primer: 5'-CACACACACACACACACACAAGC-3' T_m: 59.148
Reverse Primer: 5'-ACACAGGATGGGAACCAGTCCTA-3' T_m: 59.148
Expected PCR Product Length: 370
Expected CelI Cleavage Product Sizes: 240, 130
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr7:8187550-8187607

Rank: 137	Homology Score: 10
	Genomic Region: Intron	Closest Gene: LINC00299
Match Type: R25R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GCTTT TGCACCTGCCCCCAGT         GCTGTAAGTTCTATGGAAATTCCAC          CCTGGTGTCAGCTCCA CACTG-3'
	3'-CGAAA ACGTGGACGGGGGTCA         CGACATTCAAGATACCTTTAAGGTG          GGACCACAGTCGAGGT GTGAC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 002-008374518
Forward Primer: 5'-TCCCGGAAACATGTCAGCCC-3' T_m: 59.508
Reverse Primer: 5'-CATCCTACATCCCCTGAACATGG-3' T_m: 59.148
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 139, 131
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr2:8374627-8374683

Rank: 138	Homology Score: 10
	Genomic Region: Intron	Closest Gene: ME1
Match Type: R22R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CTGTT TGCACTTGACATATCT           GAATAAGATTTCTTTAAATAAG           ACAGGAGTGTCGTGCA CTGAT-3'
	3'-GACAA ACGTGAACTGTATAGA           CTTATTCTAAAGAAATTTATTC           TGTCCTCACAGCACGT GACTA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 006-083995632
Forward Primer: 5'-CAGTTGTTCGGATACGGCTTATCTTCATG-3' T_m: 60.178
Reverse Primer: 5'-GGGTAAGAAAACAGAGGCACAGAG-3' T_m: 59.038
Expected PCR Product Length: 307
Expected CelI Cleavage Product Sizes: 171, 136
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr6:83995774-83995827

Rank: 139	Homology Score: 10
	Genomic Region: Intron	Closest Gene: RERE
Match Type: R16R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GGTGC TGGACCTGACCCCTAT              CTGCAGTAGCAAGAGA              GCAGAAGACAGGGGCT AAGCT-3'
	3'-CCACG ACCTGGACTGGGGATA              GACGTCATCGTTCTCT              CGTCTTCTGTCCCCGA TTCGA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 001-008489463
Forward Primer: 5'-GGATAGAGAATACTGGGCTGAGAG-3' T_m: 59.038
Reverse Primer: 5'-GTAAGAGGACTGAGTAGATGTCCC-3' T_m: 59.038
Expected PCR Product Length: 308
Expected CelI Cleavage Product Sizes: 137, 171
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr1:8489574-8489621

Rank: 140	Homology Score: 10
	Genomic Region: Intron	Closest Gene: NXPH1
Match Type: R21R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-ATTAA TGCAACAGACTTCTGT           ACTTAGAAGAACTGCCTAAGA            AGAGAACTCAGGTTAA CTCTG-3'
	3'-TAATT ACGTTGTCTGAAGACA           TGAATCTTCTTGACGGATTCT            TCTCTTGAGTCCAATT GAGAC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 007-008646994
Forward Primer: 5'-GGATCGTGTCACTCGATACAGTAC-3' T_m: 59.038
Reverse Primer: 5'-GGGTCTAGCAACATATTCCCACAG-3' T_m: 59.038
Expected PCR Product Length: 329
Expected CelI Cleavage Product Sizes: 179, 150
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr7:8647145-8647197

Rank: 141	Homology Score: 10
	Genomic Region: Intron	Closest Gene: DAPK1
Match Type: R30R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TAAGC ACCACGTGTTTCCTGT       TATTGTTGGAACCCCGCAGACCACTCTGGA       ACAGCTGTGAGGTGCA GCAGT-3'
	3'-ATTCG TGGTGCACAAAGGACA       ATAACAACCTTGGGGCGTCTGGTGAGACCT       TGTCGACACTCCACGT CGTCA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 009-090286947
Forward Primer: 5'-CCGCTTGTCAAATGGTTGAGTCAG-3' T_m: 59.038
Reverse Primer: 5'-CAGCAACATTCCCCTCTGTAAACC-3' T_m: 59.038
Expected PCR Product Length: 302
Expected CelI Cleavage Product Sizes: 139, 163
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr9:90287053-90287114

Rank: 142	Homology Score: 10
	Genomic Region: Intron	Closest Gene: NTN1
Match Type: R16R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GGAAT TACAGAGGACTCCTGG              GGAATTAGCCACATCC              ACAGCAGCCAGGAGCA CCAGG-3'
	3'-CCTTA ATGTCTCCTGAGGACC              CCTTAATCGGTGTAGG              TGTCGTCGGTCCTCGT GGTCC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 017-009048384
Forward Primer: 5'-AGCTGAGGCAGGCGCATGAA-3' T_m: 59.508
Reverse Primer: 5'-CCCGGTGGCGTTGTTTTATGAC-3' T_m: 59.258
Expected PCR Product Length: 313
Expected CelI Cleavage Product Sizes: 131, 182
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr17:9048489-9048536

Rank: 143	Homology Score: 10
	Genomic Region: Intron	Closest Gene: TDP1
Match Type: R22R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TAAAT TCCACATCACTCCTGT           TTGGCTTCTGTTGTCAGCATTT           ACAAAAGTCTGGTCCT TTGTA-3'
	3'-ATTTA AGGTGTAGTGAGGACA           AACCGAAGACAACAGTCGTAAA           TGTTTTCAGACCAGGA AACAT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 014-090487653
Forward Primer: 5'-GGAAACAATGAAGTCACATCACTAGGAGG-3' T_m: 60.178
Reverse Primer: 5'-CCCCTATAAGGTCGGTTTAGGGA-3' T_m: 59.148
Expected PCR Product Length: 355
Expected CelI Cleavage Product Sizes: 133, 222
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr14:90487757-90487810

Rank: 144	Homology Score: 10
	Genomic Region: Intron	Closest Gene: CLMN
Match Type: R24R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CAGTA AGCTTCTGAGTACTGT          ACCTGCAGTGGCAACAGAGAATGG          ACAGGCGTCAGGGGCT ACAGC-3'
	3'-GTCAT TCGAAGACTCATGACA          TGGACGTCACCGTTGTCTCTTACC          TGTCCGCAGTCCCCGA TGTCG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 014-095659628
Forward Primer: 5'-CCAACAGTTCTCCGACAGCAAG-3' T_m: 59.258
Reverse Primer: 5'-GTTCTGAGCCTGCAGCTGTG-3' T_m: 59.508
Expected PCR Product Length: 303
Expected CelI Cleavage Product Sizes: 137, 166
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr14:95659735-95659790

Rank: 145	Homology Score: 10
	Genomic Region: Intron	Closest Gene: USP43
Match Type: R21R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TCATG TGAAACTGACTCCTGA           GAAAATGTGGGCAGGAATGAT            TTAGCAGGCAGGTGGA AGGTG-3'
	3'-AGTAC ACTTTGACTGAGGACT           CTTTTACACCCGTCCTTACTA            AATCGTCCGTCCACCT TCCAC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 017-009569400
Forward Primer: 5'-CAAGGGGAATGAAAAGGACAGCAG-3' T_m: 59.038
Reverse Primer: 5'-CCTGCGCGTTATTTCTGTCCC-3' T_m: 59.378
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 135, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr17:9569507-9569559

Rank: 146	Homology Score: 10
	Genomic Region: Intron	Closest Gene: USP43
Match Type: R28R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TCATG TGAAACTGACTCCTGA        GAAAATGTGGGCAGGAATGATTTAGCAG        GCAGGTGGAAGGTGGA GAGAT-3'
	3'-AGTAC ACTTTGACTGAGGACT        CTTTTACACCCGTCCTTACTAAATCGTC        CGTCCACCTTCCACCT CTCTA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 017-009569404
Forward Primer: 5'-GGGAATGAAAAGGACAGCAGGAC-3' T_m: 59.148
Reverse Primer: 5'-CGGCCCTGCGCGTTATTTCT-3' T_m: 59.508
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 135, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr17:9569507-9569566

Rank: 147	Homology Score: 10
	Genomic Region: Intron	Closest Gene: LOC100616530
Match Type: R24R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GAGCT TGGACCTGTCTCCTCT          GCATCTGCAGTGGAGCCACGCCTC          ACTCGACTCAGTAGCA GGTCA-3'
	3'-CTCGA ACCTGGACAGAGGAGA          CGTAGACGTCACCTCGGTGCGGAG          TGAGCTGAGTCATCGT CCAGT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 008-096438369
Forward Primer: 5'-GACCAAGCCCCAGAATGGTTAATC-3' T_m: 59.038
Reverse Primer: 5'-CAGCTCCCCCTAAACTACCAG-3' T_m: 59.378
Expected PCR Product Length: 310
Expected CelI Cleavage Product Sizes: 178, 132
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr8:96438517-96438572

Rank: 148	Homology Score: 10
	Genomic Region: Intron	Closest Gene: TBC1D23
Match Type: R23R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TGGAT GGCACATGGCTTCTGA          AAGATCGTGGGGAGTGGGTGGAG           ATAAGAGTCAGGTGAA GTTAC-3'
	3'-ACCTA CCGTGTACCGAAGACT          TTCTAGCACCCCTCACCCACCTC           TATTCTCAGTCCACTT CAATG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 003-099990183
Forward Primer: 5'-GTGGAGACGTTTTTGTGGTGGAC-3' T_m: 59.148
Reverse Primer: 5'-CTCAGCCCCCTCCAAAAACAAAC-3' T_m: 59.148
Expected PCR Product Length: 287
Expected CelI Cleavage Product Sizes: 147, 140
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr3:99990301-99990355

Rank: 149	Homology Score: 10
	Genomic Region: Intergenic	Closest Gene: LOC100129138
Match Type: R16R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CATGA TTTACCTGAATGCAGT              TCTTTCTCTCTTACCA              ACAGAAGTGAGGTTCA GGGCC-3'
	3'-GTACT AAATGGACTTACGTCA              AGAAAGAGAGAATGGT              TGTCTTCACTCCAAGT CCCGG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 001-104689409
Forward Primer: 5'-GTTGAGGGAGTCATCCTGAGC-3' T_m: 59.378
Reverse Primer: 5'-GGTTTCACTGTGTTAGCCAGGATG-3' T_m: 59.038
Expected PCR Product Length: 334
Expected CelI Cleavage Product Sizes: 204, 130
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr1:104689587-104689634

Rank: 150	Homology Score: 10
	Genomic Region: Intergenic	Closest Gene: AIM1
Match Type: R24R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CTCAG TGTCCCAGACTGCAGT          CTATGACTGTGGTGGTATGGTTTT          GCAGGGGTCAGGGGCA CTAAG-3'
	3'-GAGTC ACAGGGTCTGACGTCA          GATACTGACACCACCATACCAAAA          CGTCCCCAGTCCCCGT GATTC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 006-106935880
Forward Primer: 5'-TGGTGCATTGACTTTGGTTCTGGG-3' T_m: 59.038
Reverse Primer: 5'-TCTGACCCAGACCTGACAGATAG-3' T_m: 59.148
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 132, 138
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr6:106935982-106936037

Rank: 151	Homology Score: 10
	Genomic Region: Intergenic	Closest Gene: ABRA
Match Type: R28R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TTCTT TGGACCTGACTCCTTG        AAAGTGAGCTGTCCTTCTTATGTATCCA        AAAAGTCTCAGGTGTA TTATA-3'
	3'-AAGAA ACCTGGACTGAGGAAC        TTTCACTCGACAGGAAGAATACATAGGT        TTTTCAGAGTCCACAT AATAT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 008-107911219
Forward Primer: 5'-GGCTTCACTTTAGGTCTCTCTTCC-3' T_m: 59.038
Reverse Primer: 5'-CCATAGCAAGCCATTTCAGCCTTC-3' T_m: 59.038
Expected PCR Product Length: 401
Expected CelI Cleavage Product Sizes: 248, 153
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr8:107911435-107911494

Rank: 152	Homology Score: 10
	Genomic Region: Intergenic	Closest Gene: DOCK6
Match Type: R15R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CCAGC TGCCCCTGCCTCCTGG              ATGCTTCTCTAAACA               CCAGGACACAGCTCCA CTCAA-3'
	3'-GGTCG ACGGGGACGGAGGACC              TACGAAGAGATTTGT               GGTCCTGTGTCGAGGT GAGTT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 019-011375833
Forward Primer: 5'-GAGAGTGAGACCCTGTCTCAAAAC-3' T_m: 59.038
Reverse Primer: 5'-TAGGCTACATTAGGGCGAGTCC-3' T_m: 59.258
Expected PCR Product Length: 321
Expected CelI Cleavage Product Sizes: 180, 141
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr19:11375988-11376034

Rank: 153	Homology Score: 10
	Genomic Region: Intergenic	Closest Gene: TNFSF15
Match Type: R20R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-ATCAC CCCAGCAGACCCCTGT            GGGTTTGCTCACATAAGGGA            ACAGGAGTTATGAGCA ATGAA-3'
	3'-TAGTG GGGTCGTCTGGGGACA            CCCAAACGAGTGTATTCCCT            TGTCCTCAATACTCGT TACTT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 009-117519299
Forward Primer: 5'-CAAAAGGACCTGCATCTAAGCACC-3' T_m: 59.038
Reverse Primer: 5'-ATTGTTCTTCCCTGGCCACCC-3' T_m: 59.378
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 135, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr9:117519406-117519457

Rank: 154	Homology Score: 10
	Genomic Region: Intergenic	Closest Gene: MAN1A2
Match Type: R11R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-ATCTT TGACCCTGCCTCCTGT                CTTTATTTCAA                 AAAGGATGCAATTGCA AAGAT-3'
	3'-TAGAA ACTGGGACGGAGGACA                GAAATAAAGTT                 TTTCCTACGTTAACGT TTCTA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Warning! Primer pair was generated using suboptimal parameters. 
Primer Index: 001-118086389
Forward Primer: 5'-CTGTTCATTTTCTTAAGGTGGAGACTTACCTG-3' T_m: 59.848
Reverse Primer: 5'-GCACTCTGGAAACATTCTCTTTCAATGTGTC-3' T_m: 59.958
Expected PCR Product Length: 448
Expected CelI Cleavage Product Sizes: 270, 178
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr1:118086636-118086678

Rank: 155	Homology Score: 10
	Genomic Region: Intergenic	Closest Gene: NDST3
Match Type: R26R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TTACA TGCATCTATCTCATTT         AATGCTTTTAACAATGCTACAACTAA         AAAGTAGTCAGGTGTA GACTG-3'
	3'-AATGT ACGTAGATAGAGTAAA         TTACGAAAATTGTTACGATGTTGATT         TTTCATCAGTCCACAT CTGAC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Warning! Primer pair was generated using suboptimal parameters. 
Primer Index: 004-118702135
Forward Primer: 5'-GCAGGGACACTGGTGTTATGTCT-3' T_m: 59.148
Reverse Primer: 5'-GATAGTAACTTATTTAGAATTGAGGTTGTGTGATGGAG-3' T_m: 59.358
Expected PCR Product Length: 429
Expected CelI Cleavage Product Sizes: 293, 136
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr4:118702397-118702454

Rank: 156	Homology Score: 10
	Genomic Region: Intergenic	Closest Gene: HAS2
Match Type: R18R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CCTCT TGGACCTGACACCTCT             TCCCCAAACCAGAGGAAC             TCTGTAGCCAGGGGCA TCAAC-3'
	3'-GGAGA ACCTGGACTGTGGAGA             AGGGGTTTGGTCTCCTTG             AGACATCGGTCCCCGT AGTTG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 008-122428182
Forward Primer: 5'-ACAAGTTCTGCCAACTACACCAGG-3' T_m: 59.038
Reverse Primer: 5'-AGAGCCATTCCCAAGTGGGGT-3' T_m: 59.378
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 134, 136
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr8:122428289-122428338

Rank: 157	Homology Score: 10
	Genomic Region: Intergenic	Closest Gene: PLXNA1
Match Type: R28R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TGACT TGCAGCTCACTCCTCT        GTTCCTCTGCCACCCTGGACAGGACGGC        AGAGTGGTCAGGTGTG CCTCT-3'
	3'-ACTGA ACGTCGAGTGAGGAGA        CAAGGAGACGGTGGGACCTGTCCTGCCG        TCTCACCAGTCCACAC GGAGA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 003-126892267
Forward Primer: 5'-ACCCAGACCCAGACATCCAC-3' T_m: 59.508
Reverse Primer: 5'-CTATGTGCAGCTGGAGAAACTGAG-3' T_m: 59.038
Expected PCR Product Length: 282
Expected CelI Cleavage Product Sizes: 152, 130
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr3:126892387-126892446

Rank: 158	Homology Score: 10
	Genomic Region: Intergenic	Closest Gene: POTEE
Match Type: R29R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TTTGG TGCACCCAACACCTGT       GCAGTATACACTGTATACACTGCACCGGG        GCAGGAGCTGGCTGCA ATCCT-3'
	3'-AAACC ACGTGGGTTGTGGACA       CGTCATATGTGACATATGTGACGTGGCCC        CGTCCTCGACCGACGT TAGGA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Warning! Primer pair was generated using suboptimal parameters. 
Primer Index: 002-131974522
Forward Primer: 5'-GCTAATGGGTGCAAAGTTTCTTTTAAGGAGCATA-3' T_m: 59.668
Reverse Primer: 5'-AAGACGCCCACCGAGCCCA-3' T_m: 62.008
Expected PCR Product Length: 450
Expected CelI Cleavage Product Sizes: 309, 141
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr2:131974799-131974859

Rank: 159	Homology Score: 10
	Genomic Region: Intergenic	Closest Gene: PCDH10
Match Type: R26R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CCAAT TGCATCAGATACCTGA         TTTAGCACACTGATTAAAAGGAATGA         ACTGTAGTCAGGTTCA GTGGC-3'
	3'-GGTTA ACGTAGTCTATGGACT         AAATCGTGTGACTAATTTTCCTTACT         TGACATCAGTCCAAGT CACCG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 004-132113714
Forward Primer: 5'-GCTGTTCTGAGGTTGTTCCTGTG-3' T_m: 59.148
Reverse Primer: 5'-CCAGACTGAAGCATAGTCCCATGA-3' T_m: 59.038
Expected PCR Product Length: 291
Expected CelI Cleavage Product Sizes: 151, 140
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr4:132113834-132113891

Rank: 160	Homology Score: 10
	Genomic Region: Intergenic	Closest Gene: RXRA
Match Type: R11R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TTCCT GGCTCCTGATTCCTGT                CTTTTAGCCCA                 GCACGGGGCAGGTCCA GCAGG-3'
	3'-AAGGA CCGAGGACTAAGGACA                GAAAATCGGGT                 CGTGCCCCGTCCAGGT CGTCC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 009-137130018
Forward Primer: 5'-ATGGGGTTTGGAAGGAGATGGG-3' T_m: 59.258
Reverse Primer: 5'-AGCTCCTTCCAGAACAGGGAC-3' T_m: 59.378
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 135, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr9:137130130-137130172

Rank: 161	Homology Score: 10
	Genomic Region: Intergenic	Closest Gene: ARAP3
Match Type: R23R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TGTTA TGCTCTTGGCTCCTAG          CCAGTGGTTATATGACATCAGGG           AAAGGACACAGGTGCA TGGAG-3'
	3'-ACAAT ACGAGAACCGAGGATC          GGTCACCAATATACTGTAGTCCC           TTTCCTGTGTCCACGT ACCTC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 005-141074133
Forward Primer: 5'-TCTGCTTGGGCCTTAGAGGG-3' T_m: 59.508
Reverse Primer: 5'-ATTCAGCATCCCTGCCCCAGA-3' T_m: 59.378
Expected PCR Product Length: 292
Expected CelI Cleavage Product Sizes: 139, 153
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr5:141074243-141074297

Rank: 162	Homology Score: 10
	Genomic Region: Intergenic	Closest Gene: FLJ43860
Match Type: R24R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TTTGT TAAACCACATTCCTGT          GAGAGGCAACTTTTCAACTGGAGA          ACAGTACTCATGTGCA GTTCC-3'
	3'-AAACA ATTTGGTGTAAGGACA          CTCTCCGTTGAAAAGTTGACCTCT          TGTCATGAGTACACGT CAAGG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 008-142885698
Forward Primer: 5'-GTGAACTACCTCTCTGGCACTG-3' T_m: 59.258
Reverse Primer: 5'-CTCACCCAAAACTGGAAGCAACC-3' T_m: 59.148
Expected PCR Product Length: 336
Expected CelI Cleavage Product Sizes: 132, 204
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr8:142885800-142885855

Rank: 163	Homology Score: 10
	Genomic Region: Intergenic	Closest Gene: TONSL
Match Type: R18R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CCTTG GGTATCTGACTCCCGG             CTACACAGCACACCCAGC             ACTGCACTCAGGTGCA GTGGC-3'
	3'-GGAAC CCATAGACTGAGGGCC             GATGTGTCGTGTGGGTCG             TGACGTGAGTCCACGT CACCG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 008-145671350
Forward Primer: 5'-CAACAGAGCGAGACTCTTGTCTCA-3' T_m: 59.038
Reverse Primer: 5'-GCCCAACGACTTGCTGTAATTACG-3' T_m: 59.038
Expected PCR Product Length: 330
Expected CelI Cleavage Product Sizes: 190, 140
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr8:145671513-145671562

Rank: 164	Homology Score: 10
	Genomic Region: Intergenic	Closest Gene: AFF2
Match Type: R21R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GGATT TGAGCCTGACTTCTCA           GCCCTAATGCTCCTTTACAGG            ACAGGAGCCATGTGAA ATTAA-3'
	3'-CCTAA ACTCGGACTGAAGAGT           CGGGATTACGAGGAAATGTCC            TGTCCTCGGTACACTT TAATT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 00X-148285072
Forward Primer: 5'-GGAGCAAAAAGGACTATGTCACTGAGTCAA-3' T_m: 60.058
Reverse Primer: 5'-CCACGTAAGTCCTACAGGTGACTA-3' T_m: 59.038
Expected PCR Product Length: 450
Expected CelI Cleavage Product Sizes: 307, 143
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chrX:148285351-148285403

Rank: 165	Homology Score: 10
	Genomic Region: Intergenic	Closest Gene: DCLK2
Match Type: R24R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GCAGG TTGACTTGACTCCTTG          TGGAATCACCACAGCATGACCTGG          ACAGGTGTCAGTGGCA ACAGC-3'
	3'-CGTCC AACTGAACTGAGGAAC          ACCTTAGTGGTGTCGTACTGGACC          TGTCCACAGTCACCGT TGTCG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 004-150284205
Forward Primer: 5'-GAGGAAGTCCCCAGTGAGAAAATG-3' T_m: 59.038
Reverse Primer: 5'-TATTCCTGGACCCACCCTACTTC-3' T_m: 59.148
Expected PCR Product Length: 309
Expected CelI Cleavage Product Sizes: 179, 130
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr4:150284354-150284409

Rank: 166	Homology Score: 10
	Genomic Region: Intergenic	Closest Gene: LOC285889
Match Type: R19R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-ATTGA TGGACTTGACACCTGT            ATCCCTGGAGGGTCTGTCC             TCAGCAGTGGGCTGCA GCCCA-3'
	3'-TAACT ACCTGAACTGTGGACA            TAGGGACCTCCCAGACAGG             AGTCGTCACCCGACGT CGGGT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 007-156274056
Forward Primer: 5'-CAGGCGAGGTCTTACCCGAT-3' T_m: 59.508
Reverse Primer: 5'-GTCCACGTGTTCCCATTTTTGGC-3' T_m: 59.148
Expected PCR Product Length: 383
Expected CelI Cleavage Product Sizes: 130, 253
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr7:156274159-156274209

Rank: 167	Homology Score: 10
	Genomic Region: Intergenic	Closest Gene: LRP2
Match Type: R13R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GAACG TGCACCAGAATTCAGC               CAAGCTCCCTATC                AAAGGAGTCAGGTCTA TCTTT-3'
	3'-CTTGC ACGTGGTCTTAAGTCG               GTTCGAGGGATAG                TTTCCTCAGTCCAGAT AGAAA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 002-170227380
Forward Primer: 5'-GGCCAGAAGACCAAGATGATTCC-3' T_m: 59.148
Reverse Primer: 5'-GCCAAAAGCCAAATGTAGACTTTGTGCTG-3' T_m: 60.178
Expected PCR Product Length: 403
Expected CelI Cleavage Product Sizes: 259, 144
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr2:170227615-170227659

Rank: 168	Homology Score: 10
	Genomic Region: Intergenic	Closest Gene: MSX2
Match Type: R16R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TTTGT TGATCCTGTGTCCAGT              GCTGTGGCTGGAAATG              ACAGCAGTCACGTTCA TGCAC-3'
	3'-AAACA ACTAGGACACAGGTCA              CGACACCGACCTTTAC              TGTCGTCAGTGCAAGT ACGTG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 005-174033940
Forward Primer: 5'-CAGGAAGTCTTCCTTGACTGCAC-3' T_m: 59.148
Reverse Primer: 5'-GGCAGGCATTTTCCAGATGAGG-3' T_m: 59.258
Expected PCR Product Length: 330
Expected CelI Cleavage Product Sizes: 186, 144
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr5:174034100-174034147

Rank: 169	Homology Score: 10
	Genomic Region: Intergenic	Closest Gene: TEX35
Match Type: R19R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-AACAC AGCATCTCACTCCTGT            CAACCTGGTGGCCAATGAC             AACAGAGTGAGGGGCA GTTGG-3'
	3'-TTGTG TCGTAGAGTGAGGACA            GTTGGACCACCGGTTACTG             TTGTCTCACTCCCCGT CAACC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 001-178499007
Forward Primer: 5'-CACTTATCATGTCGAGGCCTGC-3' T_m: 59.258
Reverse Primer: 5'-CCCTCTTAATTGCTCCCAGATGAC-3' T_m: 59.038
Expected PCR Product Length: 334
Expected CelI Cleavage Product Sizes: 199, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr1:178499179-178499229

Rank: 170	Homology Score: 10
	Genomic Region: Intergenic	Closest Gene: LINC00290
Match Type: R30R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GCTTC TGCTCCGAAGTCCTGC       ATGGACATGCTTGTATATATTTATACCATT       AAAGGAGTCAGTTTCA AAAAT-3'
	3'-CGAAG ACGAGGCTTCAGGACG       TACCTGTACGAACATATATAAATATGGTAA       TTTCCTCAGTCAAAGT TTTTA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 004-182299969
Forward Primer: 5'-GACAAGGAAACTGAGGCACAGAAG-3' T_m: 59.038
Reverse Primer: 5'-ACACTGATTCCTGACCTCAGCG-3' T_m: 59.258
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 138, 132
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr4:182300074-182300135

Rank: 171	Homology Score: 10
	Genomic Region: Intergenic	Closest Gene: SH3YL1
Match Type: R19R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-AAAAG TGAACATGAGTCATAT            GTTACCTGACTTGTTCAAT             ACATGAGTCAGGACCA CCTTC-3'
	3'-TTTTC ACTTGTACTCAGTATA            CAATGGACTGAACAAGTTA             TGTACTCAGTCCTGGT GGAAG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 002-000185249
Forward Primer: 5'-CCACTGACCATAGACTGGTTCTG-3' T_m: 59.148
Reverse Primer: 5'-CCTGAACATTCCTGCAACCTTCAC-3' T_m: 59.038
Expected PCR Product Length: 392
Expected CelI Cleavage Product Sizes: 157, 235
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr2:185379-185429

Rank: 172	Homology Score: 10
	Genomic Region: Intergenic	Closest Gene: CDH18
Match Type: R22R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TCTTG ATTAAATGACTCCTGT           GATTTTTGTTTGTTTGTTTTTT           TCAGTAGTCAGGAGCA CAAAC-3'
	3'-AGAAC TAATTTACTGAGGACA           CTAAAAACAAACAAACAAAAAA           AGTCATCAGTCCTCGT GTTTG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 005-019219531
Forward Primer: 5'-GGACATTCAGACAAATCCCTAGGC-3' T_m: 59.038
Reverse Primer: 5'-GGGAGTTAAAGGAGTGAAAGATGAACACTG-3' T_m: 60.058
Expected PCR Product Length: 406
Expected CelI Cleavage Product Sizes: 152, 254
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr5:19219654-19219707

Rank: 173	Homology Score: 10
	Genomic Region: Intergenic	Closest Gene: KCNT2
Match Type: R10R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CAGAT TGGACGTGACTACTGT                 GCCCTTGCCC                 AGAGGAGTCCTATGTA TCCAT-3'
	3'-GTCTA ACCTGCACTGATGACA                 CGGGAACGGG                 TCTCCTCAGGATACAT AGGTA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 001-195936317
Forward Primer: 5'-GGACCATCAGCAGATAGTACGAAG-3' T_m: 59.038
Reverse Primer: 5'-GTTGGGGTCAGTTTCCTAGGC-3' T_m: 59.378
Expected PCR Product Length: 383
Expected CelI Cleavage Product Sizes: 251, 132
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr1:195936545-195936586

Rank: 174	Homology Score: 10
	Genomic Region: Intergenic	Closest Gene: BTG2
Match Type: R22R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TTCTG TAGGCCTGACTCCTGT           AAGGAGCCTTTGGTAAGGAAGG           TGAGGACTCAGGTGTC CTGGC-3'
	3'-AAGAC ATCCGGACTGAGGACA           TTCCTCGGAAACCATTCCTTCC           ACTCCTGAGTCCACAG GACCG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 001-203281134
Forward Primer: 5'-TGCATGCTGCAGATTAAGAGCCC-3' T_m: 59.148
Reverse Primer: 5'-TAAGAAGTACCAGGACCACAACCC-3' T_m: 59.038
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 137, 133
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr1:203281242-203281295

Rank: 175	Homology Score: 10
	Genomic Region: Intergenic	Closest Gene: LOC100505912
Match Type: R20R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TCTGC TGCTTCTGACTTCTGT            TGGGAAAGGTTCTCTTCCTT            TAAGGAGTCATGTGAT TCAAT-3'
	3'-AGACG ACGAAGACTGAAGACA            ACCCTTTCCAAGAGAAGGAA            ATTCCTCAGTACACTA AGTTA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 004-022231229
Forward Primer: 5'-CTTGGTTCTTGTACACAGGACCC-3' T_m: 59.148
Reverse Primer: 5'-CTGCAGCCTGATTCTGAACTGTG-3' T_m: 59.148
Expected PCR Product Length: 286
Expected CelI Cleavage Product Sizes: 136, 150
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr4:22231337-22231388

Rank: 176	Homology Score: 10
	Genomic Region: Intergenic	Closest Gene: MIR4686
Match Type: R25R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TCGGC TCCACCTGACTCCTAC         ACTGGCTCTGCAACCATTCCCAGCC          ACAGAGGTTGGCTGCA AGTAC-3'
	3'-AGCCG AGGTGGACTGAGGATG         TGACCGAGACGTTGGTAAGGGTCGG          TGTCTCCAACCGACGT TCATG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 011-002239013
Forward Primer: 5'-AGCTGTCCAGCCTGCAGCAT-3' T_m: 59.508
Reverse Primer: 5'-TCATATTCCTGGGTGGTCACTCC-3' T_m: 59.148
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 138, 132
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr11:2239121-2239177

Rank: 177	Homology Score: 10
	Genomic Region: Intergenic	Closest Gene: COG2
Match Type: R28R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TTATT TGAACATGACTTCTGT        GCATTCCATTGCACCATGTGGTGGGGAG        AAAGGTGTCTGGTCTA TTTTT-3'
	3'-AATAA ACTTGTACTGAAGACA        CGTAAGGTAACGTGGTACACCACCCCTC        TTTCCACAGACCAGAT AAAAA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 001-230749531
Forward Primer: 5'-CAAAGATGCAAAGGCTGATTTAGAGCACC-3' T_m: 60.178
Reverse Primer: 5'-CCCCAAATTTAGCAATACTGTCTGCTGGA-3' T_m: 60.178
Expected PCR Product Length: 441
Expected CelI Cleavage Product Sizes: 131, 310
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr1:230749630-230749689

Rank: 178	Homology Score: 10
	Genomic Region: Intergenic	Closest Gene: PPARGC1A
Match Type: R13R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-ACAAA TGCACCCTACTCATGT               AAGATGTTATAAC                AGGGGAGACTGGGGCA GGGCA-3'
	3'-TGTTT ACGTGGGATGAGTACA               TTCTACAATATTG                TCCCCTCTGACCCCGT CCCGT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 004-024170953
Forward Primer: 5'-CTGGATGGGATCCCAGAACAGA-3' T_m: 59.258
Reverse Primer: 5'-GAGCCAACATGCTTGGCTGAG-3' T_m: 59.378
Expected PCR Product Length: 276
Expected CelI Cleavage Product Sizes: 140, 136
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr4:24171069-24171113

Rank: 179	Homology Score: 10
	Genomic Region: Intergenic	Closest Gene: LOC200772
Match Type: R29R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CCCAC TGCACCTGTCACCTCT       GGCCCAGCACAAGGGCTGCATGTGGCCAC        ACCTTAGGCAGGTCCA ACCGC-3'
	3'-GGGTG ACGTGGACAGTGGAGA       CCGGGTCGTGTTCCCGACGTACACCGGTG        TGGAATCCGTCCAGGT TGGCG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 002-241889867
Forward Primer: 5'-TCAGAGCCTGAAGCAGGAGC-3' T_m: 59.508
Reverse Primer: 5'-GGGCAAGTGGATGGGTGGAT-3' T_m: 59.508
Expected PCR Product Length: 287
Expected CelI Cleavage Product Sizes: 135, 152
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr2:241889970-241890030

Rank: 180	Homology Score: 10
	Genomic Region: Intergenic	Closest Gene: DHRS2
Match Type: R10R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TCTCC TTCACCTGACTCTAGT                 TGTTGCTGGA                 ACAGGACTTTGTTGTA TATTC-3'
	3'-AGAGG AAGTGGACTGAGATCA                 ACAACGACCT                 TGTCCTGAAACAACAT ATAAG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 014-024242484
Forward Primer: 5'-CAAGTCTTCCTCAGCCATCAGACA-3' T_m: 59.038
Reverse Primer: 5'-CCAGACACATCAACAGAGTGGAAG-3' T_m: 59.038
Expected PCR Product Length: 298
Expected CelI Cleavage Product Sizes: 138, 160
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr14:24242599-24242640

Rank: 181	Homology Score: 10
	Genomic Region: Intergenic	Closest Gene: EIF3B
Match Type: R23R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CTTTG TTCTCCCGTTTCCTGT          GTATGGTTCAGCCCCAGTGAGCC           ACAGGAGACAGGGCCA GGAGC-3'
	3'-GAAAC AAGAGGGCAAAGGACA          CATACCAAGTCGGGGTCACTCGG           TGTCCTCTGTCCCGGT CCTCG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 007-002427539
Forward Primer: 5'-TTGGCCAGGCTGGTCTCGAA-3' T_m: 59.508
Reverse Primer: 5'-CTTTGATGAACCCACAGGGCG-3' T_m: 59.378
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 136, 134
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr7:2427646-2427700

Rank: 182	Homology Score: 10
	Genomic Region: Intergenic	Closest Gene: LOC100507173
Match Type: R12R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GGCCT GGCAACTGACACCTGT                AATCCCAGCTAC                TCGGGAGGCAGAGGCA GAGGC-3'
	3'-CCGGA CCGTTGACTGTGGACA                TTAGGGTCGATG                AGCCCTCCGTCTCCGT CTCCG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 006-027625538
Forward Primer: 5'-ATCCGAGAACTGTGGGAGGC-3' T_m: 59.508
Reverse Primer: 5'-CTCCCTGTTAATGCCACCCC-3' T_m: 59.508
Expected PCR Product Length: 334
Expected CelI Cleavage Product Sizes: 142, 192
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr6:27625656-27625699

Rank: 183	Homology Score: 10
	Genomic Region: Intergenic	Closest Gene: DKFZp686K1684
Match Type: R11R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GAACT TTCACCTGACTCCTTA                TGCTTAGCTCA                 AACGGGGGCAGGTTCA AGCAG-3'
	3'-CTTGA AAGTGGACTGAGGAAT                ACGAATCGAGT                 TTGCCCCCGTCCAAGT TCGTC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 011-031965225
Forward Primer: 5'-CCAGCCTGAGACAACCATCTTTAC-3' T_m: 59.038
Reverse Primer: 5'-GAAGGGCCATAACTTGGGCATTAG-3' T_m: 59.038
Expected PCR Product Length: 355
Expected CelI Cleavage Product Sizes: 192, 163
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr11:31965394-31965436

Rank: 184	Homology Score: 10
	Genomic Region: Intergenic	Closest Gene: NUBPL
Match Type: R13R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CTTTT TGCCCCTGAGTTCTGT               GGAAATCTTGGAA                ACAGGGCCCAGGAACA TATTC-3'
	3'-GAAAA ACGGGGACTCAAGACA               CCTTTAGAACCTT                TGTCCCGGGTCCTTGT ATAAG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 014-032435668
Forward Primer: 5'-GCAGTAAGCTTGTTTCCTTGGCC-3' T_m: 59.148
Reverse Primer: 5'-AACATGGGCTTCTTGCAAGGAGAG-3' T_m: 59.038
Expected PCR Product Length: 366
Expected CelI Cleavage Product Sizes: 235, 131
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr14:32435879-32435923

Rank: 185	Homology Score: 10
	Genomic Region: Intergenic	Closest Gene: FAM47A
Match Type: R10R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-AGTCT TCACCCTTACTCCTAT                 GCTTATTCTA                 ACAGGAGTTAGATGTA CTTCT-3'
	3'-TCAGA AGTGGGAATGAGGATA                 CGAATAAGAT                 TGTCCTCAATCTACAT GAAGA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 00X-034076224
Forward Primer: 5'-AGTATTGCCCTTTCTGCTAGGGAC-3' T_m: 59.038
Reverse Primer: 5'-CACTGCACACTTCTGTGGCTC-3' T_m: 59.378
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 134, 136
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chrX:34076335-34076376

Rank: 186	Homology Score: 10
	Genomic Region: Intergenic	Closest Gene: C1orf94
Match Type: R12R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GAGCC TGGATCAGCCTCCTGG                ATCAGCCAACCC                ACAGCAGTCAGGAACA TATCA-3'
	3'-CTCGG ACCTAGTCGGAGGACC                TAGTCGGTTGGG                TGTCGTCAGTCCTTGT ATAGT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 001-034895940
Forward Primer: 5'-TTACCTGGGAGGTGGAAACATGG-3' T_m: 59.148
Reverse Primer: 5'-CCTTCCTTGCTTTGTTGGTGGATG-3' T_m: 59.038
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 135, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr1:34896051-34896094

Rank: 187	Homology Score: 10
	Genomic Region: Intergenic	Closest Gene: LOC100233156
Match Type: R25R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-AGCCA TGCATCTGATGCCTGT         GAGAGTGATCTCTAGGAGCCACTGC          ACAGGGGGCAGATGAG GGAAC-3'
	3'-TCGGT ACGTAGACTACGGACA         CTCTCACTAGAGATCCTCGGTGACG          TGTCCCCCGTCTACTC CCTTG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 218-000035382
Forward Primer: 5'-AGGAGTGAGGATCCTGTTACCG-3' T_m: 59.258
Reverse Primer: 5'-CCTAGAGTCAGAGCTGTGTTCTGT-3' T_m: 59.038
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 137, 133
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chrUn_gl000218:35489-35545

Rank: 188	Homology Score: 10
	Genomic Region: Intergenic	Closest Gene: LINC00310
Match Type: R26R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CAGAA TGCACTTGACGTCGGG         TAGCACAGCAGGGTGCGGTAATGGTG         ACAAGAATCAGCTGCA GTGTG-3'
	3'-GTCTT ACGTGAACTGCAGCCC         ATCGTGTCGTCCCACGCCATTACCAC         TGTTCTTAGTCGACGT CACAC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 021-035539617
Forward Primer: 5'-GGCCTTCCTCACTATCTTACAGTC-3' T_m: 59.038
Reverse Primer: 5'-GCAAAACCGTGTCTGCCCAAATC-3' T_m: 59.148
Expected PCR Product Length: 303
Expected CelI Cleavage Product Sizes: 144, 159
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr21:35539730-35539787

Rank: 189	Homology Score: 10
	Genomic Region: Intergenic	Closest Gene: TRAPPC3
Match Type: R11R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TCCCC TGCACCTGCCTTCTCT                GGGGACAGGCC                 ACAGCCATCCAGTGCA AGGCT-3'
	3'-AGGGG ACGTGGACGGAAGAGA                CCCCTGTCCGG                 TGTCGGTAGGTCACGT TCCGA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 001-036588039
Forward Primer: 5'-GCACATGTGTGTCCACATATGGC-3' T_m: 59.148
Reverse Primer: 5'-GGGCAGAGTTTAAGGAGAAAGGTG-3' T_m: 59.038
Expected PCR Product Length: 273
Expected CelI Cleavage Product Sizes: 140, 133
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr1:36588156-36588198

Rank: 190	Homology Score: 10
	Genomic Region: Intergenic	Closest Gene: ACTR3BP5
Match Type: R13R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-AATCA TCTAACGGACTCATGT               GGAATCATCATCA                AAAGGAGTCGAGTGCA ATCAT-3'
	3'-TTAGT AGATTGCCTGAGTACA               CCTTAGTAGTAGT                TTTCCTCAGCTCACGT TAGTA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Warning! Primer pair was generated using suboptimal parameters. 
Primer Index: 010-038882221
Forward Primer: 5'-GCAATCATTGAATGGACTGGAAAGGAATCATC-3' T_m: 59.848
Reverse Primer: 5'-CCATGCAATGATGATTCCATTCGATTCCATTC-3' T_m: 59.848
Expected PCR Product Length: 336
Expected CelI Cleavage Product Sizes: 152, 184
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr10:38882349-38882393

Rank: 191	Homology Score: 10
	Genomic Region: Intergenic	Closest Gene: DAB2
Match Type: R15R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-AGAGT TGCACCTATCTGCTGT              CTATTAGAGACAAAC               TCAAGAGTCAAAGGCA CAAAT-3'
	3'-TCTCA ACGTGGATAGACGACA              GATAATCTCTGTTTG               AGTTCTCAGTTTCCGT GTTTA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 005-039782571
Forward Primer: 5'-GTTAAAGACGTGAGAGAGAAAACCAACAGC-3' T_m: 60.058
Reverse Primer: 5'-CTGAGGAACTATTCTGAGGGGC-3' T_m: 59.258
Expected PCR Product Length: 364
Expected CelI Cleavage Product Sizes: 132, 232
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr5:39782678-39782724

Rank: 192	Homology Score: 10
	Genomic Region: Intergenic	Closest Gene: PTPRT
Match Type: R27R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GGGTC TGCACCAGGCCACTCT        CCTTTCTGAGCACCGAGTGAAGGCATC         ACAGGATCCAGTTGCA GAGCA-3'
	3'-CCCAG ACGTGGTCCGGTGAGA        GGAAAGACTCGTGGCTCACTTCCGTAG         TGTCCTAGGTCAACGT CTCGT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 020-040489952
Forward Primer: 5'-GTGCCACCCACACAGCATTC-3' T_m: 59.508
Reverse Primer: 5'-GCCAGCAATGTAGAGGGACAATG-3' T_m: 59.148
Expected PCR Product Length: 313
Expected CelI Cleavage Product Sizes: 182, 131
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr20:40490103-40490161

Rank: 193	Homology Score: 10
	Genomic Region: Intergenic	Closest Gene: GRXCR1
Match Type: R25R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TTGTA TCCACCTGGCAGCTGG         TATCATGTAGCACCGCTTATCTTGC          AAAAGAGTTAGGTGCA TGGGT-3'
	3'-AACAT AGGTGGACCGTCGACC         ATAGTACATCGTGGCGAATAGAACG          TTTTCTCAATCCACGT ACCCA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 004-043460367
Forward Primer: 5'-GCTAAGAGCCTCTTGGGCTTTTG-3' T_m: 59.148
Reverse Primer: 5'-AGCAGAGGCAGAAAGGACCAG-3' T_m: 59.378
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 136, 134
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr4:43460473-43460529

Rank: 194	Homology Score: 10
	Genomic Region: Intergenic	Closest Gene: WFDC3
Match Type: R19R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-TTTCC TTCCTCTGATTACTGT            AAAAAACAGTGTTACATAA             ACAAGATTCAAGTGCA ATTTT-3'
	3'-AAAGG AAGGAGACTAATGACA            TTTTTTGTCACAATGTATT             TGTTCTAAGTTCACGT TAAAA-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 020-044380819
Forward Primer: 5'-GCTTCCAGGAATTACTCCTGTGC-3' T_m: 59.148
Reverse Primer: 5'-GGCAATTACTCAAGTGCCCAGAAG-3' T_m: 59.038
Expected PCR Product Length: 355
Expected CelI Cleavage Product Sizes: 181, 174
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr20:44380973-44381023

Rank: 195	Homology Score: 10
	Genomic Region: Intergenic	Closest Gene: SIK1
Match Type: R24R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-AGAGG TGCTCCTGACTCCTTC          CTGTTTCAACAGTCCTCAATCTGG          TCCGGAGTCAAGTCCC ACCTC-3'
	3'-TCTCC ACGAGGACTGAGGAAG          GACAAAGTTGTCAGGAGTTAGACC          AGGCCTCAGTTCAGGG TGGAG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 021-044723716
Forward Primer: 5'-GGCCTGCAGTCATATTTATACCCG-3' T_m: 59.038
Reverse Primer: 5'-TCAGCCTGTATCCCCCTACG-3' T_m: 59.508
Expected PCR Product Length: 325
Expected CelI Cleavage Product Sizes: 195, 130
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr21:44723881-44723936

Rank: 196	Homology Score: 10
	Genomic Region: Intergenic	Closest Gene: GNPDA2
Match Type: R15R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GTGGA TGCACCTGAATTGTGT              TCTGAGCTGGGAAAT               CCAGGAGTGACCTGGA GATTC-3'
	3'-CACCT ACGTGGACTTAACACA              AGACTCGACCCTTTA               GGTCCTCACTGGACCT CTAAG-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 004-044831243
Forward Primer: 5'-GCCACTATGTTTGCTTCCCAACTC-3' T_m: 59.038
Reverse Primer: 5'-CTCTACCTGGCAGCCTTCATTG-3' T_m: 59.258
Expected PCR Product Length: 284
Expected CelI Cleavage Product Sizes: 152, 132
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr4:44831370-44831416

Rank: 197	Homology Score: 10
	Genomic Region: Intergenic	Closest Gene: LOC100216001
Match Type: R14R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CTTTT TGCAACTGGCTTCTGT               TACTGGTCCACACG               GCAGAAGTCAAGGCCA CCCAG-3'
	3'-GAAAA ACGTTGACCGAAGACA               ATGACCAGGTGTGC               CGTCTTCAGTTCCGGT GGGTC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 010-004489187
Forward Primer: 5'-GGTAGCCTGCACTCATGATTCTG-3' T_m: 59.148
Reverse Primer: 5'-CTCACAGCACCTATGACGGTTC-3' T_m: 59.258
Expected PCR Product Length: 444
Expected CelI Cleavage Product Sizes: 263, 181
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr10:4489425-4489470

Rank: 198	Homology Score: 10
	Genomic Region: Intergenic	Closest Gene: GOSR2
Match Type: R16R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-GAGGC TGCAACTGACTGCTGC              CCCTGGGGAAGGTGGG              ACGGGGGCCAGATTCA CAGGG-3'
	3'-CTCCG ACGTTGACTGACGACG              GGGACCCCTTCCACCC              TGCCCCCGGTCTAAGT GTCCC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 017-045024202
Forward Primer: 5'-CTGGGTGACGAAATGAGACTCTG-3' T_m: 59.148
Reverse Primer: 5'-TTCATGTCAGGGTCCCCTGG-3' T_m: 59.508
Expected PCR Product Length: 308
Expected CelI Cleavage Product Sizes: 161, 147
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr17:45024337-45024384

Rank: 199	Homology Score: 10
	Genomic Region: Intergenic	Closest Gene: SPERT
Match Type: R30R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-CACAG TGAACCTGATTCCTAT       AGATTTCTCGGTGGCAGGGCAAGAAGGAAC       AGAGCAGGCACATGCA ATGAA-3'
	3'-GTGTC ACTTGGACTAAGGATA       TCTAAAGAGCCACCGTCCCGTTCTTCCTTG       TCTCGTCCGTGTACGT TACTT-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 013-046233753
Forward Primer: 5'-CTCCCTATGTTGTCCAGGCTAGA-3' T_m: 59.148
Reverse Primer: 5'-GCTGGGTTTGGTAGTTCTAACTCC-3' T_m: 59.038
Expected PCR Product Length: 362
Expected CelI Cleavage Product Sizes: 188, 174
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr13:46233908-46233969

Rank: 200	Homology Score: 10
	Genomic Region: Intergenic	Closest Gene: FOXK1
Match Type: R24R
	          Right Nuclease
	         TGCACCTGACTCCTGT
	5'-ATCAG GGCTGCTGACACCTGC          CAATTTGAGAAAGGTCTCTCCAGG          AGAGGAGGCAGCTGCA TTCTG-3'
	3'-TAGTC CCGACGACTGTGGACG          GTTAAACTCTTTCCAGAGAGGTCC          TCTCCTCCGTCGACGT AAGAC-5'
	                                                                     TGTCCTCAGTCCACGT
	                                                                        Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 007-004647137
Forward Primer: 5'-AGCAAGAGTGCAGGAACCACG-3' T_m: 59.378
Reverse Primer: 5'-GTGGATTTTCTCCATCCACCGC-3' T_m: 59.258
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 135, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr7:4647242-4647297

Plate format Oligo Order Form
Well Name Sequence 5' mods Internal Notes
Primer Plate #1
A1 011-005248069F GTGCCTATCAGAAACCCAAGAGTC S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
A2 011-005248069R CAATCTACTCCCAGGAGCAGG S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
B1 011-005255477F GTCAGTGCCTATCAGAAACCCAAG S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
B2 011-005255477R TCACTGGAGCAGGGAGGACA S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
C1 022-045287459F CAAAGGCTCTGCCTGCAGGA S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
C2 022-045287459R CAGGACTAAGGCCGCTCTTC S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
D1 003-058640715F CATATGAGGAAACTGAGAGGCGG S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
D2 003-058640715R CCTCGAGGAGGGCTATTCTAGA S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
E1 005-059619945F CCAAGACTTAGCTTAGCACCCAAC S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
E2 005-059619945R CATGCCTGGAGTTAGCCTCTCT S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
F1 005-111863094F AGTACACTTGGACCCAGCTTACC S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
F2 005-111863094R TGAATCCCAGGTAGCGTACCTGC S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
G1 006-123066634F GTAGTGGGATCGTGCCTGTG S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
G2 006-123066634R TCAGTACACTTGGACCCGGC S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
H1 010-111642202F CTCGCTCACAGCATAGCTGG S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
H2 010-111642202R TATGTGTGTCTGTGTTGCCACCTC S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
A3 015-082621505F TGGCTTTCACGGGTCTGTGC S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
A4 015-082621505R TAGACATGCAGCCTGGAGCC S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
B3 015-082918832F TGGCTTTCACGGGTCTGTGC S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
B4 015-082918832R TAGACATGCAGCCTGGAGCC S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
C3 003-123352705F GGTTTTAAGAAATGGGGGTCTCGC S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
C4 003-123352705R TCCCACCAGAGTATCAGATGGC S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
D3 002-205984816F GTGGGTACTGGCTGCTAAGTATG S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
D4 002-205984816R GTTCCTAGCAGCCACAGCAC S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
E3 022-024555541F CACAGCCATTCTGTCTACTCCC S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
E4 022-024555541R TGGCAGGTGGCCAGTGTCTT S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
F3 007-002962408F ACAGCGTTTGTCTCCTGGCC S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
F4 007-002962408R GCCCCTAAGCCTTGCATTCAG S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
G3 020-003459687F GCCAGTGTGCCCAGCCAATT S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
G4 020-003459687R GTGTACGTAGCTGTCTTCCCTTC S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
H3 007-003795640F CCTGACTCCTTTCACATGGCATAG S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
H4 007-003795640R TGTGTCACACGTGCTATGCCATG S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
A5 007-003795876F GAGTCCTTTCACATGGCATAGCAC S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
A6 007-003795876R ACATGTCTCACACGCACAATGCC S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
B5 019-039625781F CCTCACCAACATTTGGTACCGTC S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
B6 019-039625781R CGGAGGTTGCAGTGAGCTGA S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
C5 006-101676033F CCAGGTAACATACTTGCTGTCTGC S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
C6 006-101676033R GTCACTACTCAGTACACTTGGACC S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
D5 001-110268884F CAGGCAGGCAATCTTGATGTGG S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
D6 001-110268884R CTCCTTTTTCCTGCGTAGCAAGTG S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
E5 006-110284189F GCAATGTTGCTCCAAGAGGCC S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
E6 006-110284189R GAACTTATTCCTCCCACCTAGCTG S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
F5 002-235805535F ACCTCCACCAGACCACGAATG S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
F6 002-235805535R GACCCTTAGCAGGTCACACTC S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
G5 020-045428486F GTCTCACTGAGACCTAGGCG S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
G6 020-045428486R TGGGCCTGGGGAACCAACAA S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
H5 012-072604585F GTTGAGAGTTGACACCAACTTCTTTTCTTTTTCC S2_S5_NN_Ranking-Homology	Warning! Primer pair was generated using suboptimal parameters. 
H6 012-072604585R CCACTCCTAGTGTGGAACCTC S2_S5_NN_Ranking-Homology	Warning! Primer pair was generated using suboptimal parameters. 
A7 015-085803797F TAGACATGCAGCCTGGAGCC S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
A8 015-085803797R TGGCTTTCACGGGTCTGTGC S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
B7 017-072588236F CAGAGCGTCTCTGTCTCCAAG S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
B8 017-072588236R GGCACACCTTGCAACATACACTG S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
C7 010-100332368F GAGAGTGAACTCACCTTCCATGAC S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
C8 010-100332368R TGGGCACACAGTCAGTTCCTAG S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
D7 009-129960176F CTTCCTTGGAGTGTCCAATGGG S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
D8 009-129960176R CATCAGGGCTTTTGGCCTCAAAC S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
E7 N/A Primers Not Generated 
E8 N/A Primers Not Generated! 
F7 005-172280139F TGGTTTACCACGTGCTGTTGTTGC S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
F8 005-172280139R AAGATGCCTCTGGGTGGCCT S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
G7 004-186747672F CTGTGAGCTACTGAGGATATGCG S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
G8 004-186747672R GGGCAGATGCTTAAATCACTCCCA S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
H7 007-030006854F CCAAGAAGGTTATGCCCTGAATGC S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
H8 007-030006854R TAGTGGGTGTCCTTGGCTCTG S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
A9 003-052584959F GGCTAGGAATCTTAAGCACCCC S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
A10 003-052584959R GAGGGGATAGCAAGGCTGTG S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
B9 003-054731501F CCTACAGTTTCCAAGGACAAGCAG S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
B10 003-054731501R CCCAGCAGTAGCTTTAGCATCC S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
C9 016-071012605F CTCCTAGCTTCCTGATTCTTTGCC S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
C10 016-071012605R CCCACCCTGGTACAGCAAATG S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
D9 014-077327606F GCCTGGTATAGAACAAGCACCTG S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
D10 014-077327606R CCTCCTGTGATTGCCAGAGC S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
E9 012-081520008F GGCAGCAACTCTCCAGTGAGTA S2_S5_NN_Ranking-Homology	Warning! Primer pair was generated using suboptimal parameters. 
E10 012-081520008R CCTGTATTTCCCTATGTATATGTGCTCAGTC S2_S5_NN_Ranking-Homology	Warning! Primer pair was generated using suboptimal parameters. 
F9 002-086899302F GGCTCTGTCTTTTGTTATGTCAAATGAGAAGCA S2_S5_NN_Ranking-Homology	Warning! Primer pair was generated using suboptimal parameters. 
F10 002-086899302R CTCCATCATTAATTCAAACAGCCTTCAATACCC S2_S5_NN_Ranking-Homology	Warning! Primer pair was generated using suboptimal parameters. 
G9 014-100782711F TGAAGGGACAGAGCCAGGGT S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
G10 014-100782711R ACACCAGCCTGGGCAACAGA S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
H9 012-101038644F CTGTGCTCCCCTTTCTGAATAGTG S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
H10 012-101038644R TACACTTGGACCCGACTTCCC S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
A11 008-011765812F ATCCCCAGTAATGTACCTGCTGTC S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
A12 008-011765812R CTCAGTACACTTGCACCCGG S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
B11 008-015147764F CCCCACTTCATCTGCTAAACTTCC S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
B12 008-015147764R GAGGGGGAAAATGTGGGAAGTTCT S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
C11 00Y-018946879F ATACACTTGGACTCGGCTTCCC S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
C12 00Y-018946879R GTGAATCTCAGGTAGTGTACCTGC S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
D11 004-190659833F CTGCCTCCAATCCCTCAGAAAATG S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
D12 004-190659833R CTGGTATTGGGTCCAGACCAC S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
E11 002-199208411F GCCTTACACATAACCTCACTGCC S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
E12 002-199208411R CTCCAACATAACTCAGCCTCGAAG S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
F11 dom-000216374F TCTCCTTTCTGTACCTCAGAGACC S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
F12 dom-000216374R CCCACCCTGGTACAGCAAATG S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
G11 002-241334168F CATGCACCCCTGGCTTGTGT S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
G12 002-241334168R AGCTTTGCACCTGGAACCTGTGA S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
H11 012-032161181F GCTCAATGGCAGAAAACCAAATAACCCAAC S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
H12 012-032161181R GAGACGGGTTTTTGCCATGTTGC S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
Primer Plate #2
A1 018-055555293F TCTGGGAAGAAGCTGCCAGG S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
A2 018-055555293R GGCTATGCTACCTGCTGTAAAACC S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
B1 018-072086920F GCTGTCACCAGGAAAAACATAAGAGCC S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
B2 018-072086920R AAGCTGCTGAAATGGGCAGAGG S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
C1 019-009837811F AATCCCAGGTAGCGTACGTGC S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
C2 019-009837811R TCAGTACACATGGACCTGGCTTC S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
D1 012-129191997F GGTGGGGTATACCAGCATAAACTC S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
D2 012-129191997R GGCTTCAAGGACCCTTTCTTAGCT S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
E1 005-137519020F GCGAGGATCAAAATGGCAATCCC S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
E2 005-137519020R GGCAGAGGACTAATGTTTCCTGG S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
F1 006-138657515F GTTCCCGTGCATCAGTCAGC S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
F2 006-138657515R GCTGATCAGTGGTTGGAAAGGC S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
G1 009-033466447F AATGGGCCTAGGACTGGGAG S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
G2 009-033466447R TCTCCCTCTCAGACCTCCAG S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
H1 001-036031906F CAGAGTCTTTGGGCCCAGATC S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
H2 001-036031906R TGCAGAGAGGAGTCATAGCAGG S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
A3 012-047471859F GTGGACCATCAGTCTTTCCCC S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
A4 012-047471859R CTGAGAGGCATCTACCTTCATGG S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
B3 011-006643499F TGGGAAGCCATAGTCCCCAG S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
B4 011-006643499R ATCAATGAGTTCCCCCGTGTGG S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
C3 010-085944463F TGCAAGCTTGAGCCAGAGCC S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
C4 010-085944463R GAGGTTGGCTCATGATAGCTCTG S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
D3 001-231005091F GCGACATCCCAACAGGTAGG S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
D4 001-231005091R CGGAAGATGAGGCCATCTCAC S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
E3 019-049223033F AACCCACCTTCCTGGGGTGT S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
E4 019-049223033R GAGGCCCCTGCCTCTTTTTC S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
F3 002-101527279F CATGCCCAGCCTAATGTCTTCTTC S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
F4 002-101527279R GCCAGAGGAGGTTATTTTCTTGCC S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
G3 010-103998000F CTTCAGGTTAGTCTAGGAGGCATC S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
G4 010-103998000R TGACAGACGGGCCACCCTAT S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
H3 011-105719867F CAGATTGCGCCACTGCATTCC S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
H4 011-105719867R TGTCTCCTTCAGAACTGAGCTGG S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
A5 004-110007835F GACTGCTGGGAATTGCTCTTTGAC S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
A6 004-110007835R TCCCTCAACTTCCACTGGGC S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
B5 001-114253666F AGGTGTTCTGGGAGGGGAGT S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
B6 001-114253666R GAGTAGATGGTGGTGGTGTGG S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
C5 011-117354866F GCTCAGGCAAAGTTGTTCCCAG S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
C6 011-117354866R ATGTGCTGAGAGGCTGGTGC S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
D5 008-126184331F CTGAGTGGTGAAGAAACTTGCCC S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
D6 008-126184331R GCCAAGCTTACCTGTCTAGAACTG S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
E5 011-126353104F GAGGTCATACCCCCTTTTTTGTCC S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
E6 011-126353104R GGGCCCAGTTCTGAATCAAACAGC S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
F5 011-012784194F GCTGCCAAGTCTACAAAGCTGGA S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
F6 011-012784194R ACAACACGGACACCCAGGAG S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
G5 002-130879063F AAGACGCCCACCGAGCCCA S2_S5_NN_Ranking-Homology	Warning! Primer pair was generated using suboptimal parameters. 
G6 002-130879063R GCTAATGGGTGCAAAGTTTCTTTTAAGGAGCATA S2_S5_NN_Ranking-Homology	Warning! Primer pair was generated using suboptimal parameters. 
H5 005-131322201F CTGCATCTTCTGGAGAGTTTGCC S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
H6 005-131322201R TTGAGCGAGATGGCGTGCAG S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
A7 006-132465014F GTTGTGTTGTGGCTGCTCAGAGT S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
A8 006-132465014R CCTTCTATCCCCATCCTCTCTTTC S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
B7 010-133959783F TTTCTCTCGGCACCTCCCAC S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
B8 010-133959783R GTCCCTGCCCATGGACAAGT S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
C7 009-134936820F GCTGGTAAGTGAAGTTGATGTGGC S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
C8 009-134936820R CCAGGAGCAGCTGCGATGAA S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
D7 005-136419378F GCTATGGGAGAGCCGAAAACTG S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
D8 005-136419378R TGGAGTGGGCAAGCAAGAGC S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
E7 005-001406854F TCCATCTTCAGAACTCCATCCTCC S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
E8 005-001406854R CTCATGACGGAATATGATTCGGCC S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
F7 016-014749429F TACAGGCACACGCCACCACA S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
F8 016-014749429R CTGGCTTAAAGAGCAGATGCGG S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
G7 005-016519335F CCTAGATCAGCGGCTTTCAAAGCA S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
G8 005-016519335R GGGCTGAAAGGAGCTTTGAGG S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
H7 004-016836013F CACCTATGTGGCGTGATTGAGTGA S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
H8 004-016836013R AGACAGGGTTTCAGCATGTTGGC S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
A9 004-170092193F GACCTAAAGGTTTCTCCACACATAGTGAAG S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
A10 004-170092193R GACTGTTCTGAGATCTTGGGTGTG S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
B9 003-171470945F GACTCTGCTCAGTGGGTGTG S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
B10 003-171470945R AAGCAAGGTGGCTTATCAGTCTGG S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
C9 005-177943719F AAGCTTTCTGCCTCCTCAGGCC S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
C10 005-177943719R CACCTCAGTGACAGGCGCTT S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
D9 005-179732702F ATCTCCCGCCAGCACGTTCA S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
D10 005-179732702R GAACGAGACTCCCATCTCAACG S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
E9 009-019336805F GTCCATTGATAATCACTCTAGCACAGGTAC S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
E10 009-019336805R GTGTGACTTCCTTCCTTCCCTC S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
F9 017-020300643F GGTGACAGAGCAAGACTCCATC S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
F10 017-020300643R CTCTGTGGGTTAGTGCCTAATCTG S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
G9 002-219751327F TGTGTCTTAACGAGCCGCCC S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
G10 002-219751327R CTGACAGCTGTCATAGCCACG S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
H9 019-022376173F GGTACTCTCACTGTGGAAAACTGG S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
H10 019-022376173R GGCCAATTCTCCTCTCCTTTTTCC S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
A11 017-002270979F GAATGGATCTCTGAGCCCATTCCT S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
A12 017-002270979R CATGCCAAAGTACCAGGCCC S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
B11 001-227379954F GAGATCACTCTCACCACCCAAAAC S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
B12 001-227379954R GGAAACAGCAGAACGTTGTTGGTG S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
C11 001-230976433F GCGCCAAGGTCTTCTTGAGC S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
C12 001-230976433R GGAGAGTAGGAGACAGCACATG S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
D11 002-023872858F GTGGTCTTAGGCACCTCCTG S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
D12 002-023872858R CTCACTGTCCCCTTAACTTTCCTG S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
E11 021-027390640F CCTCCCTGCAGTATCTCATCTTTG S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
E12 021-027390640R GCAGGTCCATTCATTCCACCTC S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
F11 011-027668392F GGTGGGTCCATTCTGACTCTG S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
F12 011-027668392R TCATGATCCACCCACCTCGG S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
G11 010-029082810F CCTCTGAGCCCCATTCTCCT S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
G12 010-029082810R TAGAGATGGGGTCTCACCTTGTTG S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
H11 003-030699112F CTGGCACAGAGTGAGCACTTAATG S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
H12 003-030699112R GGTCGTCATGAGGAGAACACCTT S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
Primer Plate #3
A1 006-003193167F CACCACAACCGGCCGAGAAT S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
A2 006-003193167R CCCTGGGGGATAGTACAAAGGT S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
B1 010-035637732F GGGATGGTCTGAATCTGGACAC S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
B2 010-035637732R GCGGCCCTCTATTTTACATCCC S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
C1 008-003640891F AAGCAGTCACATGCCCCTGG S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
C2 008-003640891R CAGCAGGGTGCTTCAACCATG S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
D1 017-003965044F CGTAAGTCCTGACAACTCTACCTC S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
D2 017-003965044R GAAAGGCAGGACCCTTATCACTGT S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
E1 020-039770399F CCAGCTGGGATCCTCTGTTTC S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
E2 020-039770399R CCTAGCACTGGGCAAATGGG S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
F1 012-040234817F CCTTACAATTCACGGCTCTCAGAC S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
F2 012-040234817R CCACTGGAGTTGACAGATTTGGC S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
G1 019-040331309F ACGACCTCTAAAGCCTCCGC S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
G2 019-040331309R GCACAGCAAGACCCTGTCTC S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
H1 015-041188560F GACCCCCGTCTCTACCAAAAC S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
H2 015-041188560R CCACGCCCGGCTAACTTTTG S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
A3 008-041570215F GGGTGCAGAGAGATTTGCATGTG S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
A4 008-041570215R TGTCTCCTCCTGTCCCTGCT S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
B3 001-044350778F TGGAAGATCTCTTAGGAGGGCC S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
B4 001-044350778R GGGAAGGAGGAGACGGAAGT S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
C3 010-045378787F GGTGAGGCTCCATCCCACAA S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
C4 010-045378787R ACCCAGAGAGGGTGTGAGAG S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
D3 001-045422945F GCCCCCAAACACCACAAAGTC S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
D4 001-045422945R ATTACAGGCACATGCCACCACG S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
E3 015-045847122F CTCTCAGCATGTTTTTGAGATTTATCCATTTATGGTG S2_S5_NN_Ranking-Homology	Warning! Primer pair was generated using suboptimal parameters. 
E4 015-045847122R CAGATGCAAGAAGATCTAAAGCTGGAATAAATTAGATG S2_S5_NN_Ranking-Homology	Warning! Primer pair was generated using suboptimal parameters. 
F3 019-046327426F GTGCTTCGTTCACTGGTGAAGATG S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
F4 019-046327426R GTCCAGCTGTGGAAAAGTGATCTC S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
G3 020-048470754F GAGAGTGAATTGTCCAGGCTCATG S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
G4 020-048470754R TTAGATGGGGCGGTCTGGAG S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
H3 012-052789060F ATTCCTCCCACCTCCTGCCA S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
H4 012-052789060R TTCAGGGCCCAGAAGCCACA S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
A5 004-054438610F CCCAGGCCATTGCTTTTGATGC S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
A6 004-054438610R GGCTGCCTCTCATTTTGTCCAC S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
B5 008-059835610F GGGATCTTGGCAGAGCCTGA S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
B6 008-059835610R CAAGTCTCCACAGAAATGCCTCC S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
C5 020-059992627F TGGCCCTGTTTCTGGCTGGA S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
C6 020-059992627R AGTACACTGAAGTGCAGTCTCTGG S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
D5 011-060049460F GGGGACTTCATGTGCCGTAG S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
D6 011-060049460R GGTCTGTCTTATCAAACGCACTGG S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
E5 020-060508972F CATGGTTCTGTGGGATGCTTGG S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
E6 020-060508972R ACCATGGGCCTGATCGGGTA S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
F5 010-006225239F CGCCAACTTTGGGGTTGGCT S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
F6 010-006225239R AAGTGCTGGGATTACAGGCATGAG S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
G5 013-067512369F GGCATCAACAGGGCTTCTTAAACG S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
G6 013-067512369R CAATGTCAGTCGTTTCTGCATGAAGATAGG S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
H5 016-068387900F GCGCCAGTGGTCCTTGGATT S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
H6 016-068387900R CTGGGAATACAGGTGTGCACTAC S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
A7 015-072957610F TACTCACCTCTTGCCTTCAAGTGG S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
A8 015-072957610R CTTTTCTTGGCCCATGCCAGG S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
B7 007-073021712F GATGCGCCGCTTCCAGTAGT S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
B8 007-073021712R TGTGAAGCGGAGGAAGAGCC S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
C7 013-073489472F GCGCTCTGCTTCTAAGACACATTG S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
C8 013-073489472R CACATGGAAGAGATGATACATGCCATTACC S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
D7 00X-073723246F GTACAGTGGGGAGTCTGGAG S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
D8 00X-073723246R CAGAAGACCAGAAGTAGGCCTAAC S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
E7 009-000740828F ACACCCCCATCATGTTGGCG S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
E8 009-000740828R TTTGGTCAGCAAGGAGTGCCAG S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
F7 015-074364061F TTGGCCCATGCCAGGACTCA S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
F8 015-074364061R GCCCCTACAAGAATACTCACCTC S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
G7 018-074790610F GAGCTGTGGACTGAAGAGCAAC S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
G8 018-074790610R CACAGTCTTGGGAGGGTGCT S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
H7 017-075477142F TGTACCAGCCAGCCCTGATG S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
H8 017-075477142R TGCACACCCACAGGCCAAGA S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
A9 015-075561464F TACTCACCTCTTGCCTTCAAGTGG S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
A10 015-075561464R TTCTTGGCCCATGCCAGGAC S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
B9 015-075585740F TACTCACCTCTTGCCTTCAAGTGG S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
B10 015-075585740R TTCTTGGCCCATGCCAGGAC S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
C9 005-075922611F AGGGTAGCCCGGGAAGACTTT S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
C10 005-075922611R TAAAGCTTCCCCAAAGGCTTCCC S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
D9 016-007614298F TTGGGACGCAGAGGTGGGTA S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
D10 016-007614298R CTGGCTTGCAGATGAGTGCCT S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
E9 014-077327823F CCAACAGCTGGTAAGGATGTCAAG S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
E10 014-077327823R CTGTGGAAACACCAAAGCAGGG S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
F9 015-079217430F CCTGTGTCTGTCGTATCCCTTC S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
F10 015-079217430R GTATAGAACCGGCATCTACTCCAG S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
G9 015-079604259F TTGGAAAGGAGGGATCAGTGCAG S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
G10 015-079604259R GTGCCTATCAGCATGCGTCC S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
H9 007-008187341F CACACACACACACACACACAAGC S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
H10 007-008187341R ACACAGGATGGGAACCAGTCCTA S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
A11 002-008374518F TCCCGGAAACATGTCAGCCC S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
A12 002-008374518R CATCCTACATCCCCTGAACATGG S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
B11 006-083995632F CAGTTGTTCGGATACGGCTTATCTTCATG S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
B12 006-083995632R GGGTAAGAAAACAGAGGCACAGAG S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
C11 001-008489463F GGATAGAGAATACTGGGCTGAGAG S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
C12 001-008489463R GTAAGAGGACTGAGTAGATGTCCC S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
D11 007-008646994F GGATCGTGTCACTCGATACAGTAC S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
D12 007-008646994R GGGTCTAGCAACATATTCCCACAG S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
E11 009-090286947F CCGCTTGTCAAATGGTTGAGTCAG S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
E12 009-090286947R CAGCAACATTCCCCTCTGTAAACC S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
F11 017-009048384F AGCTGAGGCAGGCGCATGAA S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
F12 017-009048384R CCCGGTGGCGTTGTTTTATGAC S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
G11 014-090487653F GGAAACAATGAAGTCACATCACTAGGAGG S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
G12 014-090487653R CCCCTATAAGGTCGGTTTAGGGA S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
H11 014-095659628F CCAACAGTTCTCCGACAGCAAG S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
H12 014-095659628R GTTCTGAGCCTGCAGCTGTG S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
Primer Plate #4
A1 017-009569400F CAAGGGGAATGAAAAGGACAGCAG S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
A2 017-009569400R CCTGCGCGTTATTTCTGTCCC S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
B1 017-009569404F GGGAATGAAAAGGACAGCAGGAC S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
B2 017-009569404R CGGCCCTGCGCGTTATTTCT S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
C1 008-096438369F GACCAAGCCCCAGAATGGTTAATC S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
C2 008-096438369R CAGCTCCCCCTAAACTACCAG S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
D1 003-099990183F GTGGAGACGTTTTTGTGGTGGAC S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
D2 003-099990183R CTCAGCCCCCTCCAAAAACAAAC S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
E1 001-104689409F GTTGAGGGAGTCATCCTGAGC S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
E2 001-104689409R GGTTTCACTGTGTTAGCCAGGATG S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
F1 006-106935880F TGGTGCATTGACTTTGGTTCTGGG S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
F2 006-106935880R TCTGACCCAGACCTGACAGATAG S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
G1 008-107911219F GGCTTCACTTTAGGTCTCTCTTCC S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
G2 008-107911219R CCATAGCAAGCCATTTCAGCCTTC S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
H1 019-011375833F GAGAGTGAGACCCTGTCTCAAAAC S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
H2 019-011375833R TAGGCTACATTAGGGCGAGTCC S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
A3 009-117519299F CAAAAGGACCTGCATCTAAGCACC S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
A4 009-117519299R ATTGTTCTTCCCTGGCCACCC S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
B3 001-118086389F CTGTTCATTTTCTTAAGGTGGAGACTTACCTG S2_S5_NN_Ranking-Homology	Warning! Primer pair was generated using suboptimal parameters. 
B4 001-118086389R GCACTCTGGAAACATTCTCTTTCAATGTGTC S2_S5_NN_Ranking-Homology	Warning! Primer pair was generated using suboptimal parameters. 
C3 004-118702135F GCAGGGACACTGGTGTTATGTCT S2_S5_NN_Ranking-Homology	Warning! Primer pair was generated using suboptimal parameters. 
C4 004-118702135R GATAGTAACTTATTTAGAATTGAGGTTGTGTGATGGAG S2_S5_NN_Ranking-Homology	Warning! Primer pair was generated using suboptimal parameters. 
D3 008-122428182F ACAAGTTCTGCCAACTACACCAGG S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
D4 008-122428182R AGAGCCATTCCCAAGTGGGGT S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
E3 003-126892267F ACCCAGACCCAGACATCCAC S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
E4 003-126892267R CTATGTGCAGCTGGAGAAACTGAG S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
F3 002-131974522F GCTAATGGGTGCAAAGTTTCTTTTAAGGAGCATA S2_S5_NN_Ranking-Homology	Warning! Primer pair was generated using suboptimal parameters. 
F4 002-131974522R AAGACGCCCACCGAGCCCA S2_S5_NN_Ranking-Homology	Warning! Primer pair was generated using suboptimal parameters. 
G3 004-132113714F GCTGTTCTGAGGTTGTTCCTGTG S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
G4 004-132113714R CCAGACTGAAGCATAGTCCCATGA S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
H3 009-137130018F ATGGGGTTTGGAAGGAGATGGG S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
H4 009-137130018R AGCTCCTTCCAGAACAGGGAC S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
A5 005-141074133F TCTGCTTGGGCCTTAGAGGG S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
A6 005-141074133R ATTCAGCATCCCTGCCCCAGA S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
B5 008-142885698F GTGAACTACCTCTCTGGCACTG S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
B6 008-142885698R CTCACCCAAAACTGGAAGCAACC S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
C5 008-145671350F CAACAGAGCGAGACTCTTGTCTCA S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
C6 008-145671350R GCCCAACGACTTGCTGTAATTACG S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
D5 00X-148285072F GGAGCAAAAAGGACTATGTCACTGAGTCAA S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
D6 00X-148285072R CCACGTAAGTCCTACAGGTGACTA S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
E5 004-150284205F GAGGAAGTCCCCAGTGAGAAAATG S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
E6 004-150284205R TATTCCTGGACCCACCCTACTTC S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
F5 007-156274056F CAGGCGAGGTCTTACCCGAT S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
F6 007-156274056R GTCCACGTGTTCCCATTTTTGGC S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
G5 002-170227380F GGCCAGAAGACCAAGATGATTCC S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
G6 002-170227380R GCCAAAAGCCAAATGTAGACTTTGTGCTG S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
H5 005-174033940F CAGGAAGTCTTCCTTGACTGCAC S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
H6 005-174033940R GGCAGGCATTTTCCAGATGAGG S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
A7 001-178499007F CACTTATCATGTCGAGGCCTGC S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
A8 001-178499007R CCCTCTTAATTGCTCCCAGATGAC S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
B7 004-182299969F GACAAGGAAACTGAGGCACAGAAG S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
B8 004-182299969R ACACTGATTCCTGACCTCAGCG S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
C7 002-000185249F CCACTGACCATAGACTGGTTCTG S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
C8 002-000185249R CCTGAACATTCCTGCAACCTTCAC S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
D7 005-019219531F GGACATTCAGACAAATCCCTAGGC S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
D8 005-019219531R GGGAGTTAAAGGAGTGAAAGATGAACACTG S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
E7 001-195936317F GGACCATCAGCAGATAGTACGAAG S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
E8 001-195936317R GTTGGGGTCAGTTTCCTAGGC S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
F7 001-203281134F TGCATGCTGCAGATTAAGAGCCC S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
F8 001-203281134R TAAGAAGTACCAGGACCACAACCC S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
G7 004-022231229F CTTGGTTCTTGTACACAGGACCC S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
G8 004-022231229R CTGCAGCCTGATTCTGAACTGTG S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
H7 011-002239013F AGCTGTCCAGCCTGCAGCAT S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
H8 011-002239013R TCATATTCCTGGGTGGTCACTCC S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
A9 001-230749531F CAAAGATGCAAAGGCTGATTTAGAGCACC S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
A10 001-230749531R CCCCAAATTTAGCAATACTGTCTGCTGGA S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
B9 004-024170953F CTGGATGGGATCCCAGAACAGA S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
B10 004-024170953R GAGCCAACATGCTTGGCTGAG S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
C9 002-241889867F TCAGAGCCTGAAGCAGGAGC S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
C10 002-241889867R GGGCAAGTGGATGGGTGGAT S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
D9 014-024242484F CAAGTCTTCCTCAGCCATCAGACA S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
D10 014-024242484R CCAGACACATCAACAGAGTGGAAG S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
E9 007-002427539F TTGGCCAGGCTGGTCTCGAA S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
E10 007-002427539R CTTTGATGAACCCACAGGGCG S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
F9 006-027625538F ATCCGAGAACTGTGGGAGGC S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
F10 006-027625538R CTCCCTGTTAATGCCACCCC S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
G9 011-031965225F CCAGCCTGAGACAACCATCTTTAC S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
G10 011-031965225R GAAGGGCCATAACTTGGGCATTAG S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
H9 014-032435668F GCAGTAAGCTTGTTTCCTTGGCC S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
H10 014-032435668R AACATGGGCTTCTTGCAAGGAGAG S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
A11 00X-034076224F AGTATTGCCCTTTCTGCTAGGGAC S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
A12 00X-034076224R CACTGCACACTTCTGTGGCTC S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
B11 001-034895940F TTACCTGGGAGGTGGAAACATGG S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
B12 001-034895940R CCTTCCTTGCTTTGTTGGTGGATG S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
C11 218-000035382F AGGAGTGAGGATCCTGTTACCG S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
C12 218-000035382R CCTAGAGTCAGAGCTGTGTTCTGT S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
D11 021-035539617F GGCCTTCCTCACTATCTTACAGTC S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
D12 021-035539617R GCAAAACCGTGTCTGCCCAAATC S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
E11 001-036588039F GCACATGTGTGTCCACATATGGC S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
E12 001-036588039R GGGCAGAGTTTAAGGAGAAAGGTG S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
F11 010-038882221F GCAATCATTGAATGGACTGGAAAGGAATCATC S2_S5_NN_Ranking-Homology	Warning! Primer pair was generated using suboptimal parameters. 
F12 010-038882221R CCATGCAATGATGATTCCATTCGATTCCATTC S2_S5_NN_Ranking-Homology	Warning! Primer pair was generated using suboptimal parameters. 
G11 005-039782571F GTTAAAGACGTGAGAGAGAAAACCAACAGC S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
G12 005-039782571R CTGAGGAACTATTCTGAGGGGC S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
H11 020-040489952F GTGCCACCCACACAGCATTC S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
H12 020-040489952R GCCAGCAATGTAGAGGGACAATG S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
Primer Plate #5
A1 004-043460367F GCTAAGAGCCTCTTGGGCTTTTG S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
A2 004-043460367R AGCAGAGGCAGAAAGGACCAG S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
B1 020-044380819F GCTTCCAGGAATTACTCCTGTGC S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
B2 020-044380819R GGCAATTACTCAAGTGCCCAGAAG S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
C1 021-044723716F GGCCTGCAGTCATATTTATACCCG S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
C2 021-044723716R TCAGCCTGTATCCCCCTACG S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
D1 004-044831243F GCCACTATGTTTGCTTCCCAACTC S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
D2 004-044831243R CTCTACCTGGCAGCCTTCATTG S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
E1 010-004489187F GGTAGCCTGCACTCATGATTCTG S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
E2 010-004489187R CTCACAGCACCTATGACGGTTC S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
F1 017-045024202F CTGGGTGACGAAATGAGACTCTG S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
F2 017-045024202R TTCATGTCAGGGTCCCCTGG S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
G1 013-046233753F CTCCCTATGTTGTCCAGGCTAGA S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
G2 013-046233753R GCTGGGTTTGGTAGTTCTAACTCC S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
H1 007-004647137F AGCAAGAGTGCAGGAACCACG S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters. 
H2 007-004647137R GTGGATTTTCTCCATCCACCGC S2_S5_NN_Ranking-Homology	Primer pair generated using optimal parameters.
7329 total sites were located
Mismatch Types	Genomic Regions
0 and 2: 1 2 and 2: 1 3 and 3: 5 2 and 5: 18 3 and 4: 26 3 and 5: 182 4 and 4: 102 4 and 5: 1568 5 and 5: 5426	Exons: 358 Promoters: 77 Introns: 3048 Intergenic: 3846