Top Cleavage Sites for Huang_tnikb

PROGNOS Summary for Huang_tnikb_Ranking-TALENv2.0

The danRer7 genome was searched for the TALEN pair targeting:
5'- TGTTATTTTCTCCCCT NN..NN ATTTTTGAGCTGGTGGA -3'

Using these RVDs:
Left:  01NN02NG03NG04NI05NG06NG07NG08NG09HD10NG11HD12HD13HD14HD15NG
Right: 01HD02HD03NI04HD05HD06NI07NN08HD09NG10HD11NI12NI13NI14NI15NI16NG

Up to 4 mismatches were allowed in each nuclease half-site.

Allowed spacing distances were: 13 14 15 16 17 18 19

Homodimers and heterodimers of the nucleases were included in the output

106 total sites were located
Mismatch Types Genomic Regions
  0 and 0: 1
  2 and 3: 1
  2 and 4: 1
  3 and 4: 13
  4 and 4: 90
  Exons: 4
  Promoters: 3
  Introns: 29
  Intergenic: 70


The top 106 sites are ranked below according to parameters optimized for TALEN off-target prediction.
For the full output, click here.

This output will be deleted from the server one week after it is generated.  Please save your results.

Primer pairs were designed using the 400 bp of sequence on either side of the cleavage site to melt close to 60 °C

For easy visualization of cleavage on gels:
The smallest fragment will be longer than 130 bp, which is also the minimum separation distance between uncleaved and cleaved products.
The two cleavage products will be separated by a minimum size of 0 bp
To optimize assay conditions, the amplicons have a minimum length of 260 bp, a maximum length of 450 bp, and an optimal length of 270 bp.
Rank: 1	Homology Score: 50	TALEN Score: 100
	Genomic Region: Exon	Closest Gene: tnikb	Caution! This site scores very closely to the intended nuclease target. The potential for cleavage at this site is of great concern.
Match Type: R15L
	          Right Nuclease
	         TCCACCAGCTCAAAAAT
	5'-CAAGC TCCACCAGCTCAAAAAT   GCCCGCAGGATCCTT    AGGGGAGAAAATAACA GAAGA-3'
	3'-GTTCG AGGTGGTCGAGTTTTTA   CGGGCGTCCTAGGAA    TCCCCTCTTTTATTGT CTTCT-5'
	                                                TCCCCTCTTTTATTGT
	                                                    Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 024-027401962
Forward Primer: 5'-GCTGAGGGGAACACCAAACATG-3' T_m: 59.258
Reverse Primer: 5'-CCAGTATTGCAGCCCTTGTAATACACTGTT-3' T_m: 60.058
Expected PCR Product Length: 350
Expected CelI Cleavage Product Sizes: 154, 196
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr24:27402090-27402137

Rank: 2	Homology Score: 13	TALEN Score: 61.39
	Genomic Region: Intergenic	Closest Gene: cmtm6
Match Type: L13L
	           Left Nuclease
	         TGTTATTTTCTCCCCT
	5'-TCAGA TATTATTTTCTCTTCT     GCCACGCAGGAGC     AGGGACGAAAATAACA AAGTG-3'
	3'-AGTCT ATAATAAAAGAGAAGA     CGGTGCGTCCTCG     TCCCTGCTTTTATTGT TTCAC-5'
	                                                TCCCCTCTTTTATTGT
	                                                    Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 016-044051024
Forward Primer: 5'-GTGCTTAATGCACAGCGGCATC-3' T_m: 59.258
Reverse Primer: 5'-CAACCCTCACCCTAGTCTACC-3' T_m: 59.378
Expected PCR Product Length: 361
Expected CelI Cleavage Product Sizes: 205, 156
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr16:44051205-44051249

Rank: 3	Homology Score: 5	TALEN Score: 57.19
	Genomic Region: Intron	Closest Gene: foxp3a
Match Type: L18L
	           Left Nuclease
	         TGTTATTTTCTCCCCT
	5'-TTTTT TCTTCTTTTCTCTCTT  TCAACTTGTTTAGAGGCT   AGGGGTGGAAATAATA TTTGT-3'
	3'-AAAAA AGAAGAAAAGAGAGAA  AGTTGAACAAATCTCCGA   TCCCCACCTTTATTAT AAACA-5'
	                                                TCCCCTCTTTTATTGT
	                                                    Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 008-026575347
Forward Primer: 5'-GTCTTAAGGCCTTTCGATTGCAATGCAAAC-3' T_m: 60.058
Reverse Primer: 5'-GTGTGGGTGTTACCCAGCACT-3' T_m: 59.378
Expected PCR Product Length: 377
Expected CelI Cleavage Product Sizes: 247, 130
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr8:26575567-26575616

Rank: 4	Homology Score: 5	TALEN Score: 56.75
	Genomic Region: Intergenic	Closest Gene: dcaf13
Match Type: L18L
	           Left Nuclease
	         TGTTATTTTCTCCCCT
	5'-TGTGC TTTTATTTTCTCCTCA  CTCGCGGCAAGTTCAGGC   ATGGGAGTATTTAACA AGATA-3'
	3'-ACACG AAAATAAAAGAGGAGT  GAGCGCCGTTCAAGTCCG   TACCCTCATAAATTGT TCTAT-5'
	                                                TCCCCTCTTTTATTGT
	                                                    Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 016-047429150
Forward Primer: 5'-CACTCCTCAACTATAGGGGTGG-3' T_m: 59.258
Reverse Primer: 5'-CACTGATGCCTGTCGACGACT-3' T_m: 59.378
Expected PCR Product Length: 372
Expected CelI Cleavage Product Sizes: 206, 166
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr16:47429329-47429378

Rank: 5	Homology Score: 10	TALEN Score: 55.93
	Genomic Region: Intergenic	Closest Gene: vav3b
Match Type: L15L
	           Left Nuclease
	         TGTTATTTTCTCCCCT
	5'-AAAAT TGTTATTTTCTCATCT    TCTTCTGCCTTTTTT    AGGAAAGAAAACAACC CTTTC-3'
	3'-TTTTA ACAATAAAAGAGTAGA    AGAAGACGGAAAAAA    TCCTTTCTTTTGTTGG GAAAG-5'
	                                                TCCCCTCTTTTATTGT
	                                                    Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 002-015693319
Forward Primer: 5'-GCAACCGGGTAACCACACTTC-3' T_m: 59.378
Reverse Primer: 5'-GGAGACTGCAAAGAAACAAAGGCC-3' T_m: 59.038
Expected PCR Product Length: 299
Expected CelI Cleavage Product Sizes: 156, 143
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr2:15693450-15693496

Rank: 6	Homology Score: 5	TALEN Score: 54.72
	Genomic Region: Intergenic	Closest Gene: cyp3c1
Match Type: L17L
	           Left Nuclease
	         TGTTATTTTCTCCCCT
	5'-TTTTT TGTAAATTACTCCCCT   GATTCATGCAACGCATG   ATGGGACACAATAAAA AAGCA-3'
	3'-AAAAA ACATTTAATGAGGGGA   CTAAGTACGTTGCGTAC   TACCCTGTGTTATTTT TTCGT-5'
	                                                TCCCCTCTTTTATTGT
	                                                    Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 003-041144063
Forward Primer: 5'-CTGTGAATTGTGTGGAACCCTGC-3' T_m: 59.148
Reverse Primer: 5'-GGCAAAAGGCCAATAAAGTGTCCG-3' T_m: 59.038
Expected PCR Product Length: 396
Expected CelI Cleavage Product Sizes: 265, 131
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr3:41144302-41144350

Rank: 7	Homology Score: 5	TALEN Score: 53.48
	Genomic Region: Intergenic	Closest Gene: pop4
Match Type: L18L
	           Left Nuclease
	         TGTTATTTTCTCCCCT
	5'-GGTCT TCCTATTTTCTCTACT  TTGTGATATGAATGAAAA   AGGGTAGAACATAAGA GTGGA-3'
	3'-CCAGA AGGATAAAAGAGATGA  AACACTATACTTACTTTT   TCCCATCTTGTATTCT CACCT-5'
	                                                TCCCCTCTTTTATTGT
	                                                    Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 007-047515148
Forward Primer: 5'-GCACAGATCGTCTTATCCTCTCTC-3' T_m: 59.038
Reverse Primer: 5'-GGTCACTACTGCACAGGTACATG-3' T_m: 59.148
Expected PCR Product Length: 362
Expected CelI Cleavage Product Sizes: 138, 224
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr7:47515259-47515308

Rank: 8	Homology Score: 2	TALEN Score: 53.18
	Genomic Region: Intergenic	Closest Gene: gpc1
Match Type: L16L
	           Left Nuclease
	         TGTTATTTTCTCCCCT
	5'-TTAAT TATTATTTTCCCCACA   GATAAAAGGATAGTGA    AGAAAAGAAAATATCA AGGGG-3'
	3'-AATTA ATAATAAAAGGGGTGT   CTATTTTCCTATCACT    TCTTTTCTTTTATAGT TCCCC-5'
	                                                TCCCCTCTTTTATTGT
	                                                    Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 002-046852941
Forward Primer: 5'-GTGACCCCTGATGAATAAAGGGAC-3' T_m: 59.038
Reverse Primer: 5'-CAAAGGCTACCAAGGGTAACGC-3' T_m: 59.258
Expected PCR Product Length: 297
Expected CelI Cleavage Product Sizes: 167, 130
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr2:46853082-46853129

Rank: 9	Homology Score: 5	TALEN Score: 53.07
	Genomic Region: Intergenic	Closest Gene: pcdh15a
Match Type: R16L
	          Right Nuclease
	         TCCACCAGCTCAAAAAT
	5'-TTTTT TCTACCAGCCCGAAAAC  ATTTTCTCAATTTTAC    AGCAGAGAAAAGAACA TCATA-3'
	3'-AAAAA AGATGGTCGGGCTTTTG  TAAAAGAGTTAAAATG    TCGTCTCTTTTCTTGT AGTAT-5'
	                                                TCCCCTCTTTTATTGT
	                                                    Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 013-042252335
Forward Primer: 5'-CGTTGCCTGGTTTGATGATTCTCG-3' T_m: 59.038
Reverse Primer: 5'-GGGAGAAGTCGTGATTATGGGG-3' T_m: 59.258
Expected PCR Product Length: 277
Expected CelI Cleavage Product Sizes: 130, 147
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr13:42252439-42252487

Rank: 10	Homology Score: 2	TALEN Score: 53.01
	Genomic Region: Intergenic	Closest Gene: zgc:162928
Match Type: R14R
	          Right Nuclease
	         TCCACCAGCTCAAAAAT
	5'-CCCAT TCCACAAGCTCCAACAG   ACCTTTGTTGTCGT    TTTTCTGAGCTGTTTGA ATTGG-3'
	3'-GGGTA AGGTGTTCGAGGTTGTC   TGGAAACAACAGCA    AAAAGACTCGACAAACT TAACC-5'
	                                               TAAAAACTCGACCACCT
	                                                   Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 024-019588664
Forward Primer: 5'-GCTCAGACGTTCCACAATCCAACA-3' T_m: 59.038
Reverse Primer: 5'-GTGTTGGGACAACATGAAGCAATTATGTGG-3' T_m: 60.058
Expected PCR Product Length: 357
Expected CelI Cleavage Product Sizes: 131, 226
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr24:19588769-19588816

Rank: 11	Homology Score: 5	TALEN Score: 52.95
	Genomic Region: Promoter	Closest Gene: nedd1
Match Type: L18L
	           Left Nuclease
	         TGTTATTTTCTCCCCT
	5'-TTATT GGTTAGGTTCTCCCCT  AGTTGTGACATAAGTCAA   GGGTGAGTAAATGACA GAATA-3'
	3'-AATAA CCAATCCAAGAGGGGA  TCAACACTGTATTCAGTT   CCCACTCATTTACTGT CTTAT-5'
	                                                TCCCCTCTTTTATTGT
	                                                    Left Nuclease
No Primers
Primer Index: No Primers
Forward Primer: 5'-No Primers-3' T_m: No Primers
Reverse Primer: 5'-No Primers-3' T_m: No Primers
Expected PCR Product Length: No Primers
Expected CelI Cleavage Product Sizes: No Primers, No Primers
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr25:20030662-20030711

Rank: 12	Homology Score: 2	TALEN Score: 52.74
	Genomic Region: Intron	Closest Gene: ap2b1
Match Type: L14L
	           Left Nuclease
	         TGTTATTTTCTCCCCT
	5'-TTTCT TGATATTTTCCCCTAT    AATTATTGAAAATA     AGTTAAGAAAATAATA ATAAT-3'
	3'-AAAGA ACTATAAAAGGGGATA    TTAATAACTTTTAT     TCAATTCTTTTATTAT TATTA-5'
	                                                TCCCCTCTTTTATTGT
	                                                    Left Nuclease
No Primers
Primer Index: No Primers
Forward Primer: 5'-No Primers-3' T_m: No Primers
Reverse Primer: 5'-No Primers-3' T_m: No Primers
Expected PCR Product Length: No Primers
Expected CelI Cleavage Product Sizes: No Primers, No Primers
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr5:64771888-64771933

Rank: 13	Homology Score: 2	TALEN Score: 52.64
	Genomic Region: Exon	Closest Gene: crkl
Match Type: L15R
	           Left Nuclease
	         TGTTATTTTCTCCCCT
	5'-TTTGG GGTTATTTACACCTCT    CCTAAAACCGCCGCG   AATCTTGTGTTGGTGGA AAATT-3'
	3'-AAACC CCAATAAATGTGGAGA    GGATTTTGGCGGCGC   TTAGAACACAACCACCT TTTAA-5'
	                                               TAAAAACTCGACCACCT
	                                                   Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 021-017765608
Forward Primer: 5'-GGCTAGCTCAGCTAACGCGT-3' T_m: 59.508
Reverse Primer: 5'-CAAGCGACCTAGCACTTCTAGAC-3' T_m: 59.148
Expected PCR Product Length: 276
Expected CelI Cleavage Product Sizes: 146, 130
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr21:17765728-17765775

Rank: 14	Homology Score: 5	TALEN Score: 52.3
	Genomic Region: Intron	Closest Gene: tnnc1a
Match Type: L18L
	           Left Nuclease
	         TGTTATTTTCTCCCCT
	5'-GGTGG TGGAATTTTTTCCCTT  TTCAAAACCTAAAACCCA   AGAGGAGAAAAAACCA ACAAA-3'
	3'-CCACC ACCTTAAAAAAGGGAA  AAGTTTTGGATTTTGGGT   TCTCCTCTTTTTTGGT TGTTT-5'
	                                                TCCCCTCTTTTATTGT
	                                                    Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 023-004000336
Forward Primer: 5'-GCGCAAGATTCTGACAAGCTTTCG-3' T_m: 59.038
Reverse Primer: 5'-GCATGCGCTTGACGCAGAAGT-3' T_m: 59.378
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 133, 137
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr23:4000442-4000491

Rank: 15	Homology Score: 2	TALEN Score: 52.26
	Genomic Region: Intron	Closest Gene: adck1
Match Type: L15L
	           Left Nuclease
	         TGTTATTTTCTCCCCT
	5'-TTGGT TGTTCTTTTCAAACCT    CCAAAGTACAGAGTG    AGTAGAGAATATAAAA GGCGT-3'
	3'-AACCA ACAAGAAAAGTTTGGA    GGTTTCATGTCTCAC    TCATCTCTTATATTTT CCGCA-5'
	                                                TCCCCTCTTTTATTGT
	                                                    Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 017-017427769
Forward Primer: 5'-GTTTCGCCTCTCCTTTGAGGAC-3' T_m: 59.258
Reverse Primer: 5'-GCAGGTTGAGAGTGGGTGGT-3' T_m: 59.508
Expected PCR Product Length: 337
Expected CelI Cleavage Product Sizes: 198, 139
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr17:17427942-17427988

Rank: 16	Homology Score: 2	TALEN Score: 52.24
	Genomic Region: Intergenic	Closest Gene: jkamp
Match Type: L14L
	           Left Nuclease
	         TGTTATTTTCTCCCCT
	5'-ACTGC TGCTATTTTCTCTCTC    CAAAGATGTTATTG     AGGGGAGAAAGGCATA TTTAA-3'
	3'-TGACG ACGATAAAAGAGAGAG    GTTTCTACAATAAC     TCCCCTCTTTCCGTAT AAATT-5'
	                                                TCCCCTCTTTTATTGT
	                                                    Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 017-014089806
Forward Primer: 5'-CTCTGTTATTTTCCCTCTCTGCCC-3' T_m: 59.038
Reverse Primer: 5'-CCCACCTGCCCCTGAAATCT-3' T_m: 59.508
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 132, 138
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr17:14089913-14089958

Rank: 17	Homology Score: 5	TALEN Score: 52.17
	Genomic Region: Intergenic	Closest Gene: zgc:110204
Match Type: L13L
	           Left Nuclease
	         TGTTATTTTCTCCCCT
	5'-AACCA TGTCATTTTTTTCCCT     TTCAGCAAGAAAG     AGCGATGAAAACAACA GAAAG-3'
	3'-TTGGT ACAGTAAAAAAAGGGA     AAGTCGTTCTTTC     TCGCTACTTTTGTTGT CTTTC-5'
	                                                TCCCCTCTTTTATTGT
	                                                    Left Nuclease
Warning! Primer pair was generated using suboptimal parameters. 
Primer Index: 014-004719194
Forward Primer: 5'-AGCATGTTGAAATCAATAAATAGCCTACTACTGTCC-3' T_m: 59.498
Reverse Primer: 5'-GCTGAGGCGCTGGACAAAAAC-3' T_m: 59.378
Expected PCR Product Length: 284
Expected CelI Cleavage Product Sizes: 130, 154
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr14:4719300-4719344

Rank: 18	Homology Score: 2	TALEN Score: 52.03
	Genomic Region: Intergenic	Closest Gene: pkmb
Match Type: L17L
	           Left Nuclease
	         TGTTATTTTCTCCCCT
	5'-CATGT TTTTATTATGTCCCAT   GATGCTTTGTGTAAATT   ATGGGAGTAGATTACA TGAAA-3'
	3'-GTACA AAAATAATACAGGGTA   CTACGAAACACATTTAA   TACCCTCATCTAATGT ACTTT-5'
	                                                TCCCCTCTTTTATTGT
	                                                    Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 025-030215268
Forward Primer: 5'-GCATTGTGGCAACAAGCTTTCGAC-3' T_m: 59.038
Reverse Primer: 5'-CTTATGTGTATCTGTGGCCAAAGCTCAATG-3' T_m: 60.058
Expected PCR Product Length: 420
Expected CelI Cleavage Product Sizes: 144, 276
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr25:30215386-30215434

Rank: 19	Homology Score: 2	TALEN Score: 51.94
	Genomic Region: Intergenic	Closest Gene: sulf2l
Match Type: L18L
	           Left Nuclease
	         TGTTATTTTCTCCCCT
	5'-CGAAA TGATTTCTTCTCCACT  TCTCCGAAAGAGATGCAC   TGAGGAGAAAAGAAAA CACAA-3'
	3'-GCTTT ACTAAAGAAGAGGTGA  AGAGGCTTTCTCTACGTG   ACTCCTCTTTTCTTTT GTGTT-5'
	                                                TCCCCTCTTTTATTGT
	                                                    Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 023-015609480
Forward Primer: 5'-GTTTGACGGACAGTGTTTAGGAGG-3' T_m: 59.038
Reverse Primer: 5'-CGAGAGGGGGTATAAGGACAG-3' T_m: 59.378
Expected PCR Product Length: 375
Expected CelI Cleavage Product Sizes: 132, 243
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr23:15609585-15609634

Rank: 20	Homology Score: 5	TALEN Score: 51.84
	Genomic Region: Intergenic	Closest Gene: gadd45ba
Match Type: L13L
	           Left Nuclease
	         TGTTATTTTCTCCCCT
	5'-ACCTC ACATATTTTCTCCTCT     TGAATTTTTTGCA     AGAGGAGAAAATACCC TTTTT-3'
	3'-TGGAG TGTATAAAAGAGGAGA     ACTTAAAAAACGT     TCTCCTCTTTTATGGG AAAAA-5'
	                                                TCCCCTCTTTTATTGT
	                                                    Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 011-006696390
Forward Primer: 5'-CTCTTCAGAGCTCTAGCAGGATTC-3' T_m: 59.038
Reverse Primer: 5'-CACATGCACGCAGTTCTGTAGAG-3' T_m: 59.148
Expected PCR Product Length: 275
Expected CelI Cleavage Product Sizes: 144, 131
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr11:6696510-6696554

Rank: 21	Homology Score: 2	TALEN Score: 51.74
	Genomic Region: Intergenic	Closest Gene: enc1
Match Type: L13L
	           Left Nuclease
	         TGTTATTTTCTCCCCT
	5'-ACATC TGTCATTATCTCAACT     GAAACACAAAGAC     AGGGCGGGAAATATCA GAGCT-3'
	3'-TGTAG ACAGTAATAGAGTTGA     CTTTGTGTTTCTG     TCCCGCCCTTTATAGT CTCGA-5'
	                                                TCCCCTCTTTTATTGT
	                                                    Left Nuclease
Warning! Primer pair was generated using suboptimal parameters. 
Primer Index: 022-010953992
Forward Primer: 5'-GTATTTTGGTCTTAGTAGTGCTTTGAAATGCGC-3' T_m: 59.758
Reverse Primer: 5'-GCCATGCCCCCTTAAAGATGC-3' T_m: 59.378
Expected PCR Product Length: 422
Expected CelI Cleavage Product Sizes: 282, 140
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr22:10954250-10954294

Rank: 22	Homology Score: 2	TALEN Score: 51.7
	Genomic Region: Intergenic	Closest Gene: pex3
Match Type: L18L
	           Left Nuclease
	         TGTTATTTTCTCCCCT
	5'-GTTTG TGTTTTATTCTACACT  CGCTCGCACATGGAAGGT   AAGTGATAAAATAAAA AATAC-3'
	3'-CAAAC ACAAAATAAGATGTGA  GCGAGCGTGTACCTTCCA   TTCACTATTTTATTTT TTATG-5'
	                                                TCCCCTCTTTTATTGT
	                                                    Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 020-037794081
Forward Primer: 5'-GACGTATGGGAGATCTGCCCT-3' T_m: 59.378
Reverse Primer: 5'-GCTTGGTCATTTGTTTTGGGTGAACTATCC-3' T_m: 60.058
Expected PCR Product Length: 438
Expected CelI Cleavage Product Sizes: 256, 182
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr20:37794310-37794359

Rank: 23	Homology Score: 5	TALEN Score: 51.64
	Genomic Region: Intergenic	Closest Gene: zgc:85909
Match Type: L15R
	           Left Nuclease
	         TGTTATTTTCTCCCCT
	5'-AGAAG TGTTTTTTTTTCTCCT    TGTTTGGCGATGAGT   TTCTTTGAGCTGCAGGA CACAT-3'
	3'-TCTTC ACAAAAAAAAAGAGGA    ACAAACCGCTACTCA   AAGAAACTCGACGTCCT GTGTA-5'
	                                               TAAAAACTCGACCACCT
	                                                   Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 017-004427364
Forward Primer: 5'-GGAGTCCGCAGAGTTTAACTGG-3' T_m: 59.258
Reverse Primer: 5'-GAAGTTTCCTGCTCGAGGAGCT-3' T_m: 59.258
Expected PCR Product Length: 387
Expected CelI Cleavage Product Sizes: 230, 157
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr17:4427568-4427615

Rank: 24	Homology Score: 2	TALEN Score: 51.61
	Genomic Region: Intron	Closest Gene: LOC100003595
Match Type: L17L
	           Left Nuclease
	         TGTTATTTTCTCCCCT
	5'-CTTTC TATTATTTTTTCCCTA   TTATTCAACCTCCTTTT   AGGTGTCAAAATCACA GTTTT-3'
	3'-GAAAG ATAATAAAAAAGGGAT   AATAAGTTGGAGGAAAA   TCCACAGTTTTAGTGT CAAAA-5'
	                                                TCCCCTCTTTTATTGT
	                                                    Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 002-008682501
Forward Primer: 5'-CTACGGGCACTTTTATGTCCTTTGATTTCC-3' T_m: 60.058
Reverse Primer: 5'-GGAAATGACACTGATACTGTGTGACAGC-3' T_m: 60.298
Expected PCR Product Length: 450
Expected CelI Cleavage Product Sizes: 145, 305
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr2:8682620-8682668

Rank: 25	Homology Score: 2	TALEN Score: 51.58
	Genomic Region: Intergenic	Closest Gene: bms1l
Match Type: L17L
	           Left Nuclease
	         TGTTATTTTCTCCCCT
	5'-ACTAT TATTATTTTGTCACTT   TTCTTTACTGCTTTAAA   CGGTAAGATAATAACA CCAAT-3'
	3'-TGATA ATAATAAAACAGTGAA   AAGAAATGACGAAATTT   GCCATTCTATTATTGT GGTTA-5'
	                                                TCCCCTCTTTTATTGT
	                                                    Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 012-030852351
Forward Primer: 5'-TTGGACTGTGGGGGAAACCCAT-3' T_m: 59.258
Reverse Primer: 5'-GTCTTCAGTGTCACATGATCCCTC-3' T_m: 59.038
Expected PCR Product Length: 367
Expected CelI Cleavage Product Sizes: 130, 237
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr12:30852455-30852503

Rank: 26	Homology Score: 5	TALEN Score: 51.56
	Genomic Region: Intron	Closest Gene: zgc:153043
Match Type: L15L
	           Left Nuclease
	         TGTTATTTTCTCCCCT
	5'-AAAGG TGTTATTTTTTTCTCT    TCGTTAAACATAAGA    TGGGGAAAAAATAAAC AGGGG-3'
	3'-TTTCC ACAATAAAAAAAGAGA    AGCAATTTGTATTCT    ACCCCTTTTTTATTTG TCCCC-5'
	                                                TCCCCTCTTTTATTGT
	                                                    Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 007-058097761
Forward Primer: 5'-CTCTAACCTGCCTAGGTAACCC-3' T_m: 59.258
Reverse Primer: 5'-GAATGGGCAGATGAACTGATATGTCAACAC-3' T_m: 60.058
Expected PCR Product Length: 419
Expected CelI Cleavage Product Sizes: 200, 219
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr7:58097936-58097982

Rank: 27	Homology Score: 2	TALEN Score: 51.48
	Genomic Region: Intergenic	Closest Gene: trim35-28
Match Type: L13L
	           Left Nuclease
	         TGTTATTTTCTCCCCT
	5'-TGAAA AGTCATTTTCTCCTCC     ACCCACAAAGGGC     ACGAGAGAAAATGATA GAGAA-3'
	3'-ACTTT TCAGTAAAAGAGGAGG     TGGGTGTTTCCCG     TGCTCTCTTTTACTAT CTCTT-5'
	                                                TCCCCTCTTTTATTGT
	                                                    Left Nuclease
Warning! Primer pair was generated using suboptimal parameters. 
Primer Index: 016-028686098
Forward Primer: 5'-TGACGTATCCCGGGACTGC-3' T_m: 59.658
Reverse Primer: 5'-GGGGGACAATTCATATTCATAAAATCCAAGCC-3' T_m: 59.848
Expected PCR Product Length: 359
Expected CelI Cleavage Product Sizes: 136, 223
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr16:28686210-28686254

Rank: 28	Homology Score: 2	TALEN Score: 51.43
	Genomic Region: Intron	Closest Gene: pygmb
Match Type: R13L
	          Right Nuclease
	         TCCACCAGCTCAAAAAT
	5'-TTGAC TCCACCTGATCAGAATT    AAAGCCTCATTGA     AGGAGAGAAATAAATA CATGA-3'
	3'-AACTG AGGTGGACTAGTCTTAA    TTTCGGAGTAACT     TCCTCTCTTTATTTAT GTACT-5'
	                                                TCCCCTCTTTTATTGT
	                                                    Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 007-024009350
Forward Primer: 5'-GGATTCTTGCTCCAAAGATCCCAG-3' T_m: 59.038
Reverse Primer: 5'-GCGATTACCACAGTGGAATGAACC-3' T_m: 59.038
Expected PCR Product Length: 314
Expected CelI Cleavage Product Sizes: 133, 181
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr7:24009458-24009503

Rank: 29	Homology Score: 5	TALEN Score: 51.41
	Genomic Region: Intron	Closest Gene: lpp
Match Type: L14L
	           Left Nuclease
	         TGTTATTTTCTCCCCT
	5'-CCTGT TGTGTTTTTTTCCCCT    CTTACTTTTTAATT     AGAGCTGAAAACAACA TGACC-3'
	3'-GGACA ACACAAAAAAAGGGGA    GAATGAAAAATTAA     TCTCGACTTTTGTTGT ACTGG-5'
	                                                TCCCCTCTTTTATTGT
	                                                    Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 006-028310006
Forward Primer: 5'-GTGTCGGTGTGAACTTCCAGTAG-3' T_m: 59.148
Reverse Primer: 5'-CGAAAACAAGCTCAAGGCCACC-3' T_m: 59.258
Expected PCR Product Length: 279
Expected CelI Cleavage Product Sizes: 149, 130
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr6:28310130-28310175

Rank: 30	Homology Score: 2	TALEN Score: 51.36
	Genomic Region: Intergenic	Closest Gene: tdo2b
Match Type: L14L
	           Left Nuclease
	         TGTTATTTTCTCCCCT
	5'-AGCGG TTCTATTTTCCCACCT    ACTTTGGCACATAC     AGTAGAGAAAATAAGT ATTGA-3'
	3'-TCGCC AAGATAAAAGGGTGGA    TGAAACCGTGTATG     TCATCTCTTTTATTCA TAACT-5'
	                                                TCCCCTCTTTTATTGT
	                                                    Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 011-034935667
Forward Primer: 5'-CCTGTGTATGGGTAGCCTTCC-3' T_m: 59.378
Reverse Primer: 5'-GCAGTATCTCAGTACGTTTTCCTTGTGCT-3' T_m: 60.178
Expected PCR Product Length: 384
Expected CelI Cleavage Product Sizes: 202, 182
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr11:34935844-34935889

Rank: 31	Homology Score: 5	TALEN Score: 51.35
	Genomic Region: Intergenic	Closest Gene: vat1l
Match Type: L19L
	           Left Nuclease
	         TGTTATTTTCTCCCCT
	5'-TCAAT TCTTATTTTCTTCGCT  GTATGCAAATGAAGTAAAA  AAGGAAAAAAATAACT AAACC-3'
	3'-AGTTA AGAATAAAAGAAGCGA  CATACGTTTACTTCATTTT  TTCCTTTTTTTATTGA TTTGG-5'
	                                                TCCCCTCTTTTATTGT
	                                                    Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 485-000132960
Forward Primer: 5'-GCCTTTCTACACCTCCCTTTCTTC-3' T_m: 59.038
Reverse Primer: 5'-CTCTCTTCTCACCAGCAGAAGC-3' T_m: 59.258
Expected PCR Product Length: 426
Expected CelI Cleavage Product Sizes: 221, 205
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - Zv9_scaffold3485:133154-133204

Rank: 32	Homology Score: 2	TALEN Score: 51.26
	Genomic Region: Promoter	Closest Gene: ft1
Match Type: L13L
	           Left Nuclease
	         TGTTATTTTCTCCCCT
	5'-CAATA TTTTATTTTCCCTTCT     TCCATGCAACAGT     TATGGATAAAATAACA CTCAC-3'
	3'-GTTAT AAAATAAAAGGGAAGA     AGGTACGTTGTCA     ATACCTATTTTATTGT GAGTG-5'
	                                                TCCCCTCTTTTATTGT
	                                                    Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 019-006276406
Forward Primer: 5'-GATGGGACAGTATGGACGCG-3' T_m: 59.508
Reverse Primer: 5'-GACGTCCACAGCAGGGAATTTG-3' T_m: 59.258
Expected PCR Product Length: 388
Expected CelI Cleavage Product Sizes: 238, 150
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr19:6276620-6276664

Rank: 33	Homology Score: 2	TALEN Score: 51.24
	Genomic Region: Promoter	Closest Gene: tab2
Match Type: R17R
	          Right Nuclease
	         TCCACCAGCTCAAAAAT
	5'-TAAAA TACCCCTGCTGAAAAAT  CCAGCTTAAACCAGCCT  AGGTTTTAGCTGGTTGA CCAGC-3'
	3'-ATTTT ATGGGGACGACTTTTTA  GGTCGAATTTGGTCGGA  TCCAAAATCGACCAACT GGTCG-5'
	                                               TAAAAACTCGACCACCT
	                                                   Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 020-001352095
Forward Primer: 5'-GTGAGCCGGATGCAAGTGTC-3' T_m: 59.508
Reverse Primer: 5'-TGTCCAGTTTAAACCAGGCTGGTC-3' T_m: 59.038
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 135, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr20:1352203-1352253

Rank: 34	Homology Score: 2	TALEN Score: 51.18
	Genomic Region: Intron	Closest Gene: ube4b
Match Type: L16L
	           Left Nuclease
	         TGTTATTTTCTCCCCT
	5'-TGTCC TGTTAGCTTATCCCAT   CATTACAGCGATTGAA    AAGGGTGACAATGACA AGCCA-3'
	3'-ACAGG ACAATCGAATAGGGTA   GTAATGTCGCTAACTT    TTCCCACTGTTACTGT TCGGT-5'
	                                                TCCCCTCTTTTATTGT
	                                                    Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 023-029796044
Forward Primer: 5'-ACGACGGCAAGTAAGTGGTCC-3' T_m: 59.378
Reverse Primer: 5'-CTCAGTCCTGAAGAGGTAAGGC-3' T_m: 59.258
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 133, 137
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr23:29796151-29796198

Rank: 35	Homology Score: 2	TALEN Score: 51.15
	Genomic Region: Intergenic	Closest Gene: rdh14a
Match Type: L19L
	           Left Nuclease
	         TGTTATTTTCTCCCCT
	5'-ACTAC AGTTCTTTTTCCCCCT  TGGTGCAGTTCGTTTGGGC  AGGTGTGAAGGTAACA ATTGC-3'
	3'-TGATG TCAAGAAAAAGGGGGA  ACCACGTCAAGCAAACCCG  TCCACACTTCCATTGT TAACG-5'
	                                                TCCCCTCTTTTATTGT
	                                                    Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 013-032722528
Forward Primer: 5'-TGGACTGATTACACACACATGGCC-3' T_m: 59.038
Reverse Primer: 5'-TTGCGGTTGGCTCTGTTTTAGTGC-3' T_m: 59.038
Expected PCR Product Length: 269
Expected CelI Cleavage Product Sizes: 135, 134
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr13:32722636-32722686

Rank: 36	Homology Score: 2	TALEN Score: 51.11
	Genomic Region: Intergenic	Closest Gene: nrp2b
Match Type: L15L
	           Left Nuclease
	         TGTTATTTTCTCCCCT
	5'-GCAAA TTTTCTTTGCTTCCCT    CTGTTAAACAGAAAT    TGGGGAAAAAGTAAAA CAAGG-3'
	3'-CGTTT AAAAGAAACGAAGGGA    GACAATTTGTCTTTA    ACCCCTTTTTCATTTT GTTCC-5'
	                                                TCCCCTCTTTTATTGT
	                                                    Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 009-014999443
Forward Primer: 5'-CTTCATGGAATGCCGGATGCC-3' T_m: 59.378
Reverse Primer: 5'-GGCCCATGTAGTCTCAGGATAAG-3' T_m: 59.148
Expected PCR Product Length: 391
Expected CelI Cleavage Product Sizes: 203, 188
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr9:14999621-14999667

Rank: 37	Homology Score: 2	TALEN Score: 51.08
	Genomic Region: Intron	Closest Gene: pcdh17
Match Type: R19R
	          Right Nuclease
	         TCCACCAGCTCAAAAAT
	5'-GATTA TCCAGCAGCAAAAATAT TTTGTTGATAGAGTCTCAT ATGTTTGAATTGGTGGT GCATT-3'
	3'-CTAAT AGGTCGTCGTTTTTATA AAACAACTATCTCAGAGTA TACAAACTTAACCACCA CGTAA-5'
	                                               TAAAAACTCGACCACCT
	                                                   Right Nuclease
Warning! Primer pair was generated using suboptimal parameters. 
Primer Index: 011-033515570
Forward Primer: 5'-CAGAATTTGTGTTTGCAGAACATTTGCATGAGG-3' T_m: 59.758
Reverse Primer: 5'-GGTGCCCAAAAATCTCCATATAGTTTTTCCACA-3' T_m: 59.758
Expected PCR Product Length: 330
Expected CelI Cleavage Product Sizes: 130, 200
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr11:33515672-33515724

Rank: 38	Homology Score: 2	TALEN Score: 51.07
	Genomic Region: Intergenic	Closest Gene: zgc:103697
Match Type: L15L
	           Left Nuclease
	         TGTTATTTTCTCCCCT
	5'-AAACT TATTTTTTTCTTCTCT    CTGTTAAACAACAAT    TGGGAAAAAAATAAAA AAGGG-3'
	3'-TTTGA ATAAAAAAAGAAGAGA    GACAATTTGTTGTTA    ACCCTTTTTTTATTTT TTCCC-5'
	                                                TCCCCTCTTTTATTGT
	                                                    Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 005-037597563
Forward Primer: 5'-CTTGCCTGTCTTGCCTTGTTAACC-3' T_m: 59.038
Reverse Primer: 5'-CACAGAAACGCCAACCGACC-3' T_m: 59.508
Expected PCR Product Length: 317
Expected CelI Cleavage Product Sizes: 186, 131
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr5:37597724-37597770

Rank: 39	Homology Score: 2	TALEN Score: 51.04
	Genomic Region: Intergenic	Closest Gene: ppm1db
Match Type: L19L
	           Left Nuclease
	         TGTTATTTTCTCCCCT
	5'-GCACA AGTTAGTTTATCCTCT  TGGTACGGTTAATTTTGGC  AGGTGTGAACATAGCA ATCCC-3'
	3'-CGTGT TCAATCAAATAGGAGA  ACCATGCCAATTAAAACCG  TCCACACTTGTATCGT TAGGG-5'
	                                                TCCCCTCTTTTATTGT
	                                                    Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 005-058179155
Forward Primer: 5'-CAAGCAGTCATTCTGACCACTCTC-3' T_m: 59.038
Reverse Primer: 5'-CCACAAACGAAGTGCTACAGGG-3' T_m: 59.258
Expected PCR Product Length: 374
Expected CelI Cleavage Product Sizes: 241, 133
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr5:58179369-58179419

Rank: 40	Homology Score: 2	TALEN Score: 51.02
	Genomic Region: Intron	Closest Gene: arrb2b
Match Type: L17L
	           Left Nuclease
	         TGTTATTTTCTCCCCT
	5'-CAAAA AGTTATATGCACCCCT   GTTTAGAGCATGGACTT   AGGGGAGAACCTGAAA ATGTG-3'
	3'-GTTTT TCAATATACGTGGGGA   CAAATCTCGTACCTGAA   TCCCCTCTTGGACTTT TACAC-5'
	                                                TCCCCTCTTTTATTGT
	                                                    Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 007-027053585
Forward Primer: 5'-CGAGCTGACATATCAGCGATCTCT-3' T_m: 59.038
Reverse Primer: 5'-GGCTAAATGCCTTCTGTCTCCATC-3' T_m: 59.038
Expected PCR Product Length: 418
Expected CelI Cleavage Product Sizes: 139, 279
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr7:27053698-27053746

Rank: 41	Homology Score: 2	TALEN Score: 50.91
	Genomic Region: Intron	Closest Gene: sema4ba
Match Type: L15R
	           Left Nuclease
	         TGTTATTTTCTCCCCT
	5'-AGTCT TCTTATTTTCTCTTCA    TTGGGTGTTCATTGT   ATATTTTGGGTGGTGGA CAAGA-3'
	3'-TCAGA AGAATAAAAGAGAAGT    AACCCACAAGTAACA   TATAAAACCCACCACCT GTTCT-5'
	                                               TAAAAACTCGACCACCT
	                                                   Right Nuclease
Warning! Primer pair was generated using suboptimal parameters. 
Primer Index: 018-025634847
Forward Primer: 5'-GAGACTAAAAGAGAAGAGCTGGTAAAACAGG-3' T_m: 59.958
Reverse Primer: 5'-CTCCCCAGCTTCGCATATGTTTC-3' T_m: 59.148
Expected PCR Product Length: 289
Expected CelI Cleavage Product Sizes: 131, 158
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr18:25634952-25634999

Rank: 42	Homology Score: 2	TALEN Score: 50.9
	Genomic Region: Intergenic	Closest Gene: diras1a
Match Type: L15L
	           Left Nuclease
	         TGTTATTTTCTCCCCT
	5'-GTAAC TCTTATTTTCAACACT    TTGAACCAACAAAGC    AGAGAAAAAGATAACA GTGAC-3'
	3'-CATTG AGAATAAAAGTTGTGA    AACTTGGTTGTTTCG    TCTCTTTTTCTATTGT CACTG-5'
	                                                TCCCCTCTTTTATTGT
	                                                    Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 011-007452947
Forward Primer: 5'-GGTGCATAGGATGGGAATCATCTG-3' T_m: 59.038
Reverse Primer: 5'-GAGTGGGTGTTGAGAGTGTTGATC-3' T_m: 59.038
Expected PCR Product Length: 371
Expected CelI Cleavage Product Sizes: 160, 211
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr11:7453082-7453128

Rank: 43	Homology Score: 2	TALEN Score: 50.9
	Genomic Region: Intergenic	Closest Gene: gata3
Match Type: L16L
	           Left Nuclease
	         TGTTATTTTCTCCCCT
	5'-TGTGT TGAGATTTTTTCCCCC   TCACTAAACAGAAATT    GGGGAAGAAAATAAAT AGGGG-3'
	3'-ACACA ACTCTAAAAAAGGGGG   AGTGATTTGTCTTTAA    CCCCTTCTTTTATTTA TCCCC-5'
	                                                TCCCCTCTTTTATTGT
	                                                    Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 004-023758407
Forward Primer: 5'-GCCCTAACTTGCCTAGTTGACC-3' T_m: 59.258
Reverse Primer: 5'-GCTAAGGCTTAGAACGTGAACACAAGCA-3' T_m: 60.298
Expected PCR Product Length: 361
Expected CelI Cleavage Product Sizes: 204, 157
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr4:23758585-23758632

Rank: 44	Homology Score: 2	TALEN Score: 50.9
	Genomic Region: Intergenic	Closest Gene: gata3
Match Type: L16L
	           Left Nuclease
	         TGTTATTTTCTCCCCT
	5'-CCCCT ATTTATTTTCTTCCCC   AATTTCTGTTTAGTGA    GGGGGAAAAAATCTCA ACACA-3'
	3'-GGGGA TAAATAAAAGAAGGGG   TTAAAGACAAATCACT    CCCCCTTTTTTAGAGT TGTGT-5'
	                                                TCCCCTCTTTTATTGT
	                                                    Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 004-024096464
Forward Primer: 5'-GCTAAGGCTTAGAACGTGAACACAAGCA-3' T_m: 60.298
Reverse Primer: 5'-GCCCTAACTTGCCTAGTTGACC-3' T_m: 59.258
Expected PCR Product Length: 361
Expected CelI Cleavage Product Sizes: 161, 200
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr4:24096599-24096646

Rank: 45	Homology Score: 2	TALEN Score: 50.85
	Genomic Region: Intron	Closest Gene: pdlim5a
Match Type: L13R
	           Left Nuclease
	         TGTTATTTTCTCCCCT
	5'-CTCTG TGTTGTTTTCACCATT     AGTTATTTCTCCT    TTTTTTCAGCTGACGGA TGCCG-3'
	3'-GAGAC ACAACAAAAGTGGTAA     TCAATAAAGAGGA    AAAAAAGTCGACTGCCT ACGGC-5'
	                                               TAAAAACTCGACCACCT
	                                                   Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 005-008593040
Forward Primer: 5'-GTGGACGCGGATCGTTTTCATTC-3' T_m: 59.148
Reverse Primer: 5'-GCTTTAATCTTGTTCTGGGCCTCC-3' T_m: 59.038
Expected PCR Product Length: 340
Expected CelI Cleavage Product Sizes: 208, 132
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr5:8593223-8593268

Rank: 46	Homology Score: 2	TALEN Score: 50.82
	Genomic Region: Intergenic	Closest Gene: zmiz1a
Match Type: L13L
	           Left Nuclease
	         TGTTATTTTCTCCCCT
	5'-GTTGA TTTTTTTTTCTCTCCG     TTAAACAGAAATT     GGGGGGGAAATAAACA GTGGG-3'
	3'-CAACT AAAAAAAAAGAGAGGC     AATTTGTCTTTAA     CCCCCCCTTTATTTGT CACCC-5'
	                                                TCCCCTCTTTTATTGT
	                                                    Left Nuclease
No Primers
Primer Index: No Primers
Forward Primer: 5'-No Primers-3' T_m: No Primers
Reverse Primer: 5'-No Primers-3' T_m: No Primers
Expected PCR Product Length: No Primers
Expected CelI Cleavage Product Sizes: No Primers, No Primers
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr13:30646772-30646816

Rank: 47	Homology Score: 2	TALEN Score: 50.82
	Genomic Region: Intergenic	Closest Gene: zgc:92502
Match Type: L15L
	           Left Nuclease
	         TGTTATTTTCTCCCCT
	5'-CCTCT GTTTATTTTTTCCCCA    ATTTCTGTTTAACGG    AGGGAAGAATTTATCA ACACA-3'
	3'-GGAGA CAAATAAAAAAGGGGT    TAAAGACAAATTGCC    TCCCTTCTTAAATAGT TGTGT-5'
	                                                TCCCCTCTTTTATTGT
	                                                    Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 011-042909323
Forward Primer: 5'-GCAATTAGAGGGATACACACAGGG-3' T_m: 59.038
Reverse Primer: 5'-CCCAATTACCCTATCCTGCCTAGT-3' T_m: 59.038
Expected PCR Product Length: 411
Expected CelI Cleavage Product Sizes: 204, 207
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr11:42909502-42909548

Rank: 48	Homology Score: 2	TALEN Score: 50.63
	Genomic Region: Intergenic	Closest Gene: N/A
Match Type: L14L
	           Left Nuclease
	         TGTTATTTTCTCCCCT
	5'-ATGTT TGTTTTTTTCAGACCT    AATTAAAGAGAATG     AGGGGAGTAAAGAGAA GGAAC-3'
	3'-TACAA ACAAAAAAAGTCTGGA    TTAATTTCTCTTAC     TCCCCTCATTTCTCTT CCTTG-5'
	                                                TCCCCTCTTTTATTGT
	                                                    Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 296-000012696
Forward Primer: 5'-CTCTGAAAACTGGACTGACACGG-3' T_m: 59.148
Reverse Primer: 5'-ACTCTTTCCACACTGAGGGCAGG-3' T_m: 61.088
Expected PCR Product Length: 410
Expected CelI Cleavage Product Sizes: 280, 130
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - Zv9_NA296:12951-12996

Rank: 49	Homology Score: 2	TALEN Score: 50.6
	Genomic Region: Intergenic	Closest Gene: oep
Match Type: L15L
	           Left Nuclease
	         TGTTATTTTCTCCCCT
	5'-AAAAC TATTATGTTCTTCTCT    CAGTTAAACAGAAAT    TGGGGAAAAAATAAAC AGGGC-3'
	3'-TTTTG ATAATACAAGAAGAGA    GTCAATTTGTCTTTA    ACCCCTTTTTTATTTG TCCCG-5'
	                                                TCCCCTCTTTTATTGT
	                                                    Left Nuclease
Warning! Primer pair was generated using suboptimal parameters. 
Primer Index: 010-002176503
Forward Primer: 5'-CTGCCTAGTTAACCTAGTTACGCC-3' T_m: 59.038
Reverse Primer: 5'-GCAATGCTGTTCTTTTGAAGCTGCTATTCATC-3' T_m: 59.848
Expected PCR Product Length: 410
Expected CelI Cleavage Product Sizes: 166, 244
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr10:2176644-2176690

Rank: 50	Homology Score: 2	TALEN Score: 50.52
	Genomic Region: Intergenic	Closest Gene: tmed5
Match Type: R13L
	          Right Nuclease
	         TCCACCAGCTCAAAAAT
	5'-TATTT TCTGCCTGCTCAAAAGT    TCAAAGGCAGAAA     AGGGGAAAAAATATAT ACCAT-3'
	3'-ATAAA AGACGGACGAGTTTTCA    AGTTTCCGTCTTT     TCCCCTTTTTTATATA TGGTA-5'
	                                                TCCCCTCTTTTATTGT
	                                                    Left Nuclease
No Primers
Primer Index: No Primers
Forward Primer: 5'-No Primers-3' T_m: No Primers
Reverse Primer: 5'-No Primers-3' T_m: No Primers
Expected PCR Product Length: No Primers
Expected CelI Cleavage Product Sizes: No Primers, No Primers
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr20:14695212-14695257

Rank: 51	Homology Score: 2	TALEN Score: 50.52
	Genomic Region: Exon	Closest Gene: tln2
Match Type: R18L
	          Right Nuclease
	         TCCACCAGCTCAAAAAT
	5'-TCAGC TTCAGCAGCTCAAAGAG AACTCGTAGCCCAGGGCA   AGGTAAGAAAATAATG ACAAT-3'
	3'-AGTCG AAGTCGTCGAGTTTCTC TTGAGCATCGGGTCCCGT   TCCATTCTTTTATTAC TGTTA-5'
	                                                TCCCCTCTTTTATTGT
	                                                    Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 007-031277548
Forward Primer: 5'-GGAGTGCACAGTGTTCTCACTTG-3' T_m: 59.148
Reverse Primer: 5'-CACGCACGCACACAACACAAC-3' T_m: 59.378
Expected PCR Product Length: 426
Expected CelI Cleavage Product Sizes: 295, 131
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr7:31277816-31277866

Rank: 52	Homology Score: 2	TALEN Score: 50.5
	Genomic Region: Intergenic	Closest Gene: lmo4b
Match Type: L14L
	           Left Nuclease
	         TGTTATTTTCTCCCCT
	5'-TAGCA TGGTAGCTTCTCCACT    CACTAATCTGACAA     AGGGGGGAAAATCCCT AATCA-3'
	3'-ATCGT ACCATCGAAGAGGTGA    GTGATTAGACTGTT     TCCCCCCTTTTAGGGA TTAGT-5'
	                                                TCCCCTCTTTTATTGT
	                                                    Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 006-025723296
Forward Primer: 5'-GAGGGCATGGCTAAGTACAAACTG-3' T_m: 59.038
Reverse Primer: 5'-GTGCCAATCCCCGTATTAGATGC-3' T_m: 59.148
Expected PCR Product Length: 311
Expected CelI Cleavage Product Sizes: 153, 158
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr6:25723424-25723469

Rank: 53	Homology Score: 2	TALEN Score: 50.36
	Genomic Region: Intergenic	Closest Gene: spcs3
Match Type: L16L
	           Left Nuclease
	         TGTTATTTTCTCCCCT
	5'-AAATG TGTAAATTTCTTCTCT   CAGTTAAACGGAAATT    GGGGGAAAAATAAACA GAGGG-3'
	3'-TTTAC ACATTTAAAGAAGAGA   GTCAATTTGCCTTTAA    CCCCCTTTTTATTTGT CTCCC-5'
	                                                TCCCCTCTTTTATTGT
	                                                    Left Nuclease
Warning! Primer pair was generated using suboptimal parameters. Warning! The sequence of this region of the genome is poorly mapped. 
Primer Index: 001-039182385
Forward Primer: 5'-GCCTTTAAATGTCACTTTAAGCTGTATAGAAGTGTC-3' T_m: 59.498
Reverse Primer: 5'-GGTGGGGGGTTATAGTTTTGTAGC-3' T_m: 59.038
Expected PCR Product Length: 306
Expected CelI Cleavage Product Sizes: 162, 144
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr1:39182521-39182568

Rank: 54	Homology Score: 2	TALEN Score: 50.33
	Genomic Region: Intron	Closest Gene: wnt2bb
Match Type: L15L
	           Left Nuclease
	         TGTTATTTTCTCCCCT
	5'-CGCCT GTTTATTTTTTCCCCA    ATTTTTGTTTAACGG    AGAGAAGAAAATTTCA ACACA-3'
	3'-GCGGA CAAATAAAAAAGGGGT    TAAAAACAAATTGCC    TCTCTTCTTTTAAAGT TGTGT-5'
	                                                TCCCCTCTTTTATTGT
	                                                    Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 008-028609709
Forward Primer: 5'-CCGACACTGGGTATTCGTGTAG-3' T_m: 59.258
Reverse Primer: 5'-GCCTTATTATCCTAACCTGCCAAGTTAACC-3' T_m: 60.058
Expected PCR Product Length: 388
Expected CelI Cleavage Product Sizes: 180, 208
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr8:28609864-28609910

Rank: 55	Homology Score: 2	TALEN Score: 50.33
	Genomic Region: Intron	Closest Gene: tbck
Match Type: L15L
	           Left Nuclease
	         TGTTATTTTCTCCCCT
	5'-TGTGT TGAAATTTTCTTCTCT    CTGTTAAACAGAATT    TGGGGAAAAAATAAAC AAGCG-3'
	3'-ACACA ACTTTAAAAGAAGAGA    GACAATTTGTCTTAA    ACCCCTTTTTTATTTG TTCGC-5'
	                                                TCCCCTCTTTTATTGT
	                                                    Left Nuclease
Warning! Primer pair was generated using suboptimal parameters. 
Primer Index: 001-050760345
Forward Primer: 5'-CTCGTCGGCAGAGAATAAGGTG-3' T_m: 59.258
Reverse Primer: 5'-GTCATATGTGCCATCATTGGTTTGAACTTAGC-3' T_m: 59.848
Expected PCR Product Length: 325
Expected CelI Cleavage Product Sizes: 163, 162
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr1:50760483-50760529

Rank: 56	Homology Score: 2	TALEN Score: 50.3
	Genomic Region: Intron	Closest Gene: zgc:174650
Match Type: L14L
	           Left Nuclease
	         TGTTATTTTCTCCCCT
	5'-GTTGC TCCTCTTTACTCCCCT    CGTTCTCTTTAATT     AGGTCTGAAAAAAACA AACAT-3'
	3'-CAACG AGGAGAAATGAGGGGA    GCAAGAGAAATTAA     TCCAGACTTTTTTTGT TTGTA-5'
	                                                TCCCCTCTTTTATTGT
	                                                    Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 004-034644828
Forward Primer: 5'-CTTTCCACACTGAGGGCAGG-3' T_m: 59.508
Reverse Primer: 5'-CTGAAAACTGGGCTGACACGG-3' T_m: 59.378
Expected PCR Product Length: 407
Expected CelI Cleavage Product Sizes: 131, 276
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr4:34644934-34644979

Rank: 57	Homology Score: 2	TALEN Score: 50.3
	Genomic Region: Intergenic	Closest Gene: zgc:174275
Match Type: L14L
	           Left Nuclease
	         TGTTATTTTCTCCCCT
	5'-GTTCC TCCTCTTTACTCCCCT    CATTATCTTCAATT     AGGTCTGAAAAAAACA TTAGT-3'
	3'-CAAGG AGGAGAAATGAGGGGA    GTAATAGAAGTTAA     TCCAGACTTTTTTTGT AATCA-5'
	                                                TCCCCTCTTTTATTGT
	                                                    Left Nuclease
Warning! Primer pair was generated using suboptimal parameters. 
Primer Index: 004-057253541
Forward Primer: 5'-GCTTGCCAAACTCTTTCCACACTG-3' T_m: 59.038
Reverse Primer: 5'-CTATACCTTCTATGTTAAGCGGCTTTGACAC-3' T_m: 59.958
Expected PCR Product Length: 372
Expected CelI Cleavage Product Sizes: 134, 238
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr4:57253650-57253695

Rank: 58	Homology Score: 2	TALEN Score: 50.27
	Genomic Region: Intergenic	Closest Gene: mettl14
Match Type: L15L
	           Left Nuclease
	         TGTTATTTTCTCCCCT
	5'-TACAA TGTCATTTTCTATCAT    ATTTTGTATGATGAG    AGCGTAAAAAATTACA AGTTT-3'
	3'-ATGTT ACAGTAAAAGATAGTA    TAAAACATACTACTC    TCGCATTTTTTAATGT TCAAA-5'
	                                                TCCCCTCTTTTATTGT
	                                                    Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 001-019043719
Forward Primer: 5'-CGCAAGAAGGGGCGAATTTAACC-3' T_m: 59.148
Reverse Primer: 5'-CCGCCATTTGGGCCAAATTTTCC-3' T_m: 59.148
Expected PCR Product Length: 293
Expected CelI Cleavage Product Sizes: 145, 148
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr1:19043839-19043885

Rank: 59	Homology Score: 2	TALEN Score: 50.16
	Genomic Region: Intergenic	Closest Gene: nmrk2
Match Type: R15R
	          Right Nuclease
	         TCCACCAGCTCAAAAAT
	5'-TTTTT TCCTGCATCTCAGAAAT   CAACGGGCTTTATTA   ATCTTTGATCTAGTGAA AAAAG-3'
	3'-AAAAA AGGACGTAGAGTCTTTA   GTTGCCCGAAATAAT   TAGAAACTAGATCACTT TTTTC-5'
	                                               TAAAAACTCGACCACCT
	                                                   Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 002-056190023
Forward Primer: 5'-GGTCAAATCCAACTGTTGGCTCATAAAGTG-3' T_m: 60.058
Reverse Primer: 5'-CCGGGTGACCAGGGACTATA-3' T_m: 59.508
Expected PCR Product Length: 278
Expected CelI Cleavage Product Sizes: 139, 139
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr2:56190136-56190184

Rank: 60	Homology Score: 2	TALEN Score: 50.1
	Genomic Region: Intergenic	Closest Gene: LOC559950
Match Type: L17L
	           Left Nuclease
	         TGTTATTTTCTCCCCT
	5'-TCCCC TGTTTATTTTTCCCCC   CATTTTCTGTCTAACGG   AGAGCAGAAAATTTCA ACACA-3'
	3'-AGGGG ACAAATAAAAAGGGGG   GTAAAAGACAGATTGCC   TCTCGTCTTTTAAAGT TGTGT-5'
	                                                TCCCCTCTTTTATTGT
	                                                    Left Nuclease
No Primers
Primer Index: No Primers
Forward Primer: 5'-No Primers-3' T_m: No Primers
Reverse Primer: 5'-No Primers-3' T_m: No Primers
Expected PCR Product Length: No Primers
Expected CelI Cleavage Product Sizes: No Primers, No Primers
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr10:42410951-42410999

Rank: 61	Homology Score: 2	TALEN Score: 50.08
	Genomic Region: Intron	Closest Gene: zgc:194578
Match Type: R15L
	          Right Nuclease
	         TCCACCAGCTCAAAAAT
	5'-ATCAA TACAATTGCTCAAAAAT   ATTCTTTTATATCTT    TGGGGATAAGAAAACA GTGTC-3'
	3'-TAGTT ATGTTAACGAGTTTTTA   TAAGAAAATATAGAA    ACCCCTATTCTTTTGT CACAG-5'
	                                                TCCCCTCTTTTATTGT
	                                                    Left Nuclease
No Primers
Primer Index: No Primers
Forward Primer: 5'-No Primers-3' T_m: No Primers
Reverse Primer: 5'-No Primers-3' T_m: No Primers
Expected PCR Product Length: No Primers
Expected CelI Cleavage Product Sizes: No Primers, No Primers
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr19:11016703-11016750

Rank: 62	Homology Score: 2	TALEN Score: 50.03
	Genomic Region: Intron	Closest Gene: nrxn3b
Match Type: L13L
	           Left Nuclease
	         TGTTATTTTCTCCCCT
	5'-AAATA TGTAATCTTCTCTCCG     TTAAACAGAAATT     GGGGGAAAAAATAAAC AGGGG-3'
	3'-TTTAT ACATTAGAAGAGAGGC     AATTTGTCTTTAA     CCCCCTTTTTTATTTG TCCCC-5'
	                                                TCCCCTCTTTTATTGT
	                                                    Left Nuclease
Primer pair generated using optimal parameters. Warning! The sequence of this region of the genome is poorly mapped. 
Primer Index: 020-005773433
Forward Primer: 5'-CCCTAACCTGCCTAGTTACCC-3' T_m: 59.378
Reverse Primer: 5'-GGGGGCTCAACTCCTTATAAGGT-3' T_m: 59.148
Expected PCR Product Length: 331
Expected CelI Cleavage Product Sizes: 198, 133
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr20:5773607-5773651

Rank: 63	Homology Score: 2	TALEN Score: 49.97
	Genomic Region: Intron	Closest Gene: rbm41
Match Type: R13L
	          Right Nuclease
	         TCCACCAGCTCAAAAAT
	5'-CACAG TTGACCAGCTCAGAAAA    TGACACTCAGAAG     AGGGAAGAAAAGTGCA GAGAT-3'
	3'-GTGTC AACTGGTCGAGTCTTTT    ACTGTGAGTCTTC     TCCCTTCTTTTCACGT CTCTA-5'
	                                                TCCCCTCTTTTATTGT
	                                                    Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 021-036802701
Forward Primer: 5'-GACCGCATTTTTCTCCTCCTCAC-3' T_m: 59.148
Reverse Primer: 5'-CCGGGTTTGTGCGTTTTAAGTCC-3' T_m: 59.148
Expected PCR Product Length: 308
Expected CelI Cleavage Product Sizes: 174, 134
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr21:36802850-36802895

Rank: 64	Homology Score: 2	TALEN Score: 49.95
	Genomic Region: Intergenic	Closest Gene: t54l
Match Type: L14L
	           Left Nuclease
	         TGTTATTTTCTCCCCT
	5'-AAATT AGTTATCTTCTCTCCG    TTAAACAGAAATTG     GGGGGAAAAATAAACA GGGGG-3'
	3'-TTTAA TCAATAGAAGAGAGGC    AATTTGTCTTTAAC     CCCCCTTTTTATTTGT CCCCC-5'
	                                                TCCCCTCTTTTATTGT
	                                                    Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 008-037798987
Forward Primer: 5'-GCCTAATTACCCTAACCCGCC-3' T_m: 59.378
Reverse Primer: 5'-CTGCATGGGGTGGTAGCTTTTC-3' T_m: 59.258
Expected PCR Product Length: 375
Expected CelI Cleavage Product Sizes: 177, 198
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr8:37799139-37799184

Rank: 65	Homology Score: 2	TALEN Score: 49.95
	Genomic Region: Intergenic	Closest Gene: trps1
Match Type: L19L
	           Left Nuclease
	         TGTTATTTTCTCCCCT
	5'-TATAT TATTTTTTCCTCTCCT  CGAAAAACAAAAATAGAGG  ACGGGAGAAAAACACT TTCAC-3'
	3'-ATATA ATAAAAAAGGAGAGGA  GCTTTTTGTTTTTATCTCC  TGCCCTCTTTTTGTGA AAGTG-5'
	                                                TCCCCTCTTTTATTGT
	                                                    Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 019-047559875
Forward Primer: 5'-CTGGAAGAACGGCTCAAGGTG-3' T_m: 59.378
Reverse Primer: 5'-GACTTTAGCTGTTCTCCACATGGTTTGC-3' T_m: 60.298
Expected PCR Product Length: 305
Expected CelI Cleavage Product Sizes: 174, 131
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr19:47560022-47560072

Rank: 66	Homology Score: 2	TALEN Score: 49.92
	Genomic Region: Intergenic	Closest Gene: tpst1
Match Type: L17L
	           Left Nuclease
	         TGTTATTTTCTCCCCT
	5'-AAAAG TTTTATTTTGTCACTT   TTGACTAACTTGTTGAA   AGGGCAACAAATAATA ATTTG-3'
	3'-TTTTC AAAATAAAACAGTGAA   AACTGATTGAACAACTT   TCCCGTTGTTTATTAT TAAAC-5'
	                                                TCCCCTCTTTTATTGT
	                                                    Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 015-019562303
Forward Primer: 5'-CCTAGAGCTGGTGTCTTGCTTTC-3' T_m: 59.148
Reverse Primer: 5'-GGTCCATGCAGGTTCCAATAGG-3' T_m: 59.258
Expected PCR Product Length: 309
Expected CelI Cleavage Product Sizes: 162, 147
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr15:19562439-19562487

Rank: 67	Homology Score: 2	TALEN Score: 49.91
	Genomic Region: Intergenic	Closest Gene: rchy1
Match Type: L18R
	           Left Nuclease
	         TGTTATTTTCTCCCCT
	5'-CTTTA TAATATTTTTTGCCCT  GTTAAATAATTTATTTTT  ATTATTCAGCTGTGGGA ACGGG-3'
	3'-GAAAT ATTATAAAAAACGGGA  CAATTTATTAAATAAAAA  TAATAAGTCGACACCCT TGCCC-5'
	                                               TAAAAACTCGACCACCT
	                                                   Right Nuclease
Warning! Primer pair was generated using suboptimal parameters. 
Primer Index: 005-009640443
Forward Primer: 5'-CGCAATTCGGACTTTATAACTTGCAATTCTGAC-3' T_m: 59.758
Reverse Primer: 5'-GTGTTGGGACAAGATGAAAGAATAGTTTGGC-3' T_m: 59.958
Expected PCR Product Length: 402
Expected CelI Cleavage Product Sizes: 236, 166
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr5:9640652-9640702

Rank: 68	Homology Score: 2	TALEN Score: 49.91
	Genomic Region: Intergenic	Closest Gene: tal2
Match Type: L18R
	           Left Nuclease
	         TGTTATTTTCTCCCCT
	5'-CTTTA TAATATTTTTTGCCCT  GTTAAATAATTTATTTTT  ATTATTCAGCTGTGGGA ACGGG-3'
	3'-GAAAT ATTATAAAAAACGGGA  CAATTTATTAAATAAAAA  TAATAAGTCGACACCCT TGCCC-5'
	                                               TAAAAACTCGACCACCT
	                                                   Right Nuclease
Warning! Primer pair was generated using suboptimal parameters. 
Primer Index: 005-009701307
Forward Primer: 5'-CGCAATTCGGACTTTATAACTTGCAATTCTGAC-3' T_m: 59.758
Reverse Primer: 5'-GTTGGGACAAGATGAAGGAATAGTTTGGC-3' T_m: 60.178
Expected PCR Product Length: 379
Expected CelI Cleavage Product Sizes: 215, 164
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr5:9701495-9701545

Rank: 69	Homology Score: 2	TALEN Score: 49.87
	Genomic Region: Intergenic	Closest Gene: calr
Match Type: L15L
	           Left Nuclease
	         TGTTATTTTCTCCCCT
	5'-TGTAC TGTTATTTTATCTTCA    TCATCAAACACACAA    AGAGAACAAAATATCA TCATT-3'
	3'-ACATG ACAATAAAATAGAAGT    AGTAGTTTGTGTGTT    TCTCTTGTTTTATAGT AGTAA-5'
	                                                TCCCCTCTTTTATTGT
	                                                    Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 022-004365783
Forward Primer: 5'-GGTGTTTGTGATGGTCAGAGATCC-3' T_m: 59.038
Reverse Primer: 5'-GAAGTAACTTGCACAACACTGCATTCAACG-3' T_m: 60.058
Expected PCR Product Length: 377
Expected CelI Cleavage Product Sizes: 241, 136
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr22:4365999-4366045

Rank: 70	Homology Score: 2	TALEN Score: 49.86
	Genomic Region: Intron	Closest Gene: ntm
Match Type: L13L
	           Left Nuclease
	         TGTTATTTTCTCCCCT
	5'-ACATT TTTTTTTTTCTCTCCG     TTAAACAGAAGCT     GGGGGAAAAATAAACA AGGGG-3'
	3'-TGTAA AAAAAAAAAGAGAGGC     AATTTGTCTTCGA     CCCCCTTTTTATTTGT TCCCC-5'
	                                                TCCCCTCTTTTATTGT
	                                                    Left Nuclease
No Primers
Primer Index: No Primers
Forward Primer: 5'-No Primers-3' T_m: No Primers
Reverse Primer: 5'-No Primers-3' T_m: No Primers
Expected PCR Product Length: No Primers
Expected CelI Cleavage Product Sizes: No Primers, No Primers
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr10:31372361-31372405

Rank: 71	Homology Score: 2	TALEN Score: 49.75
	Genomic Region: Intergenic	Closest Gene: kcnd3
Match Type: L19L
	           Left Nuclease
	         TGTTATTTTCTCCCCT
	5'-TTTAA TGTTTTTTGCCACCCT  TCAGAGACACACTGAAAAT  ACGGGAGAAAATACAT AAAAA-3'
	3'-AAATT ACAAAAAACGGTGGGA  AGTCTCTGTGTGACTTTTA  TGCCCTCTTTTATGTA TTTTT-5'
	                                                TCCCCTCTTTTATTGT
	                                                    Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 008-028840626
Forward Primer: 5'-CGTCAAACATCATCAGGGAAACGC-3' T_m: 59.038
Reverse Primer: 5'-GCGAGCACACTATACACTATGCAC-3' T_m: 59.038
Expected PCR Product Length: 449
Expected CelI Cleavage Product Sizes: 267, 182
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr8:28840866-28840916

Rank: 72	Homology Score: 2	TALEN Score: 49.62
	Genomic Region: Intergenic	Closest Gene: fbxo30a
Match Type: R18L
	          Right Nuclease
	         TCCACCAGCTCAAAAAT
	5'-TGTGG TCCTCGTGGTCAAAAAT GACCACCATAGGAAACGA   ATGGGAGATAATCAAA AATAT-3'
	3'-ACACC AGGAGCACCAGTTTTTA CTGGTGGTATCCTTTGCT   TACCCTCTATTAGTTT TTATA-5'
	                                                TCCCCTCTTTTATTGT
	                                                    Left Nuclease
Warning! Primer pair was generated using suboptimal parameters. 
Primer Index: 017-007362226
Forward Primer: 5'-CAGGATCATTGGAATGGTCTAAAACTCAGTG-3' T_m: 59.958
Reverse Primer: 5'-CCCTTTTCGCAGTGCTGTACAAC-3' T_m: 59.148
Expected PCR Product Length: 338
Expected CelI Cleavage Product Sizes: 151, 187
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr17:7362350-7362400

Rank: 73	Homology Score: 2	TALEN Score: 49.6
	Genomic Region: Intergenic	Closest Gene: adpgk
Match Type: L13L
	           Left Nuclease
	         TGTTATTTTCTCCCCT
	5'-AGGTT TCTTTTTTTAACCCCT     TTATGAAAAACAA     AAGGAAAAAAATGACA TAATT-3'
	3'-TCCAA AGAAAAAAATTGGGGA     AATACTTTTTGTT     TTCCTTTTTTTACTGT ATTAA-5'
	                                                TCCCCTCTTTTATTGT
	                                                    Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 025-002697030
Forward Primer: 5'-TGTGGCTCAGAGACACACACACAT-3' T_m: 59.038
Reverse Primer: 5'-GCGACCCCTGATTAATAAAGGGAC-3' T_m: 59.038
Expected PCR Product Length: 309
Expected CelI Cleavage Product Sizes: 130, 179
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr25:2697136-2697180

Rank: 74	Homology Score: 2	TALEN Score: 49.52
	Genomic Region: Intergenic	Closest Gene: spag9a
Match Type: L13R
	           Left Nuclease
	         TGTTATTTTCTCCCCT
	5'-TTTTT TGTTAATTTTACCCTT     CACAAAAGACTTG    AATTATGAGCAGATGGA CAATT-3'
	3'-AAAAA ACAATTAAAATGGGAA     GTGTTTTCTGAAC    TTAATACTCGTCTACCT GTTAA-5'
	                                               TAAAAACTCGACCACCT
	                                                   Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 003-018061176
Forward Primer: 5'-GGTCCTCTCCTAACATTCCTTCTG-3' T_m: 59.038
Reverse Primer: 5'-GCTGCTGCTGACACATTCAGG-3' T_m: 59.378
Expected PCR Product Length: 423
Expected CelI Cleavage Product Sizes: 204, 219
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr3:18061355-18061400

Rank: 75	Homology Score: 2	TALEN Score: 49.51
	Genomic Region: Intergenic	Closest Gene: skor1b
Match Type: L17L
	           Left Nuclease
	         TGTTATTTTCTCCCCT
	5'-CCCCT GTTTATTTTTTCCCCA   ATTTCTGTTTAACCATG   ATGACAGTAAATAACA TTTGA-3'
	3'-GGGGA CAAATAAAAAAGGGGT   TAAAGACAAATTGGTAC   TACTGTCATTTATTGT AAACT-5'
	                                                TCCCCTCTTTTATTGT
	                                                    Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 018-019742010
Forward Primer: 5'-GAGCAGAAAGAGGGGGAAGATC-3' T_m: 59.258
Reverse Primer: 5'-CAATAACTTCCCTAATTACCCTAACCTGCC-3' T_m: 60.058
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 136, 134
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr18:19742120-19742168

Rank: 76	Homology Score: 2	TALEN Score: 49.45
	Genomic Region: Intergenic	Closest Gene: setd3
Match Type: L16L
	           Left Nuclease
	         TGTTATTTTCTCCCCT
	5'-TCATA TTTTATTTACTGCCTT   CTGTTTAATCGTTAAC    AGGACCGAAAAGAACA TTAAT-3'
	3'-AGTAT AAAATAAATGACGGAA   GACAAATTAGCAATTG    TCCTGGCTTTTCTTGT AATTA-5'
	                                                TCCCCTCTTTTATTGT
	                                                    Left Nuclease
Warning! Primer pair was generated using suboptimal parameters. 
Primer Index: 017-017908369
Forward Primer: 5'-TCTTGCTGCCGGAGCAGAG-3' T_m: 59.658
Reverse Primer: 5'-GATTCAGTTCAGTCATTTTTAAACCTGGCCC-3' T_m: 59.958
Expected PCR Product Length: 384
Expected CelI Cleavage Product Sizes: 138, 246
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr17:17908481-17908528

Rank: 77	Homology Score: 2	TALEN Score: 49.37
	Genomic Region: Intron	Closest Gene: gstcd
Match Type: L16L
	           Left Nuclease
	         TGTTATTTTCTCCCCT
	5'-CCCCT GTTTATTTTGTCCCCC   AATTTCTGTTTAACGG    AGAGAAGAATTTAACA CATTT-3'
	3'-GGGGA CAAATAAAACAGGGGG   TTAAAGACAAATTGCC    TCTCTTCTTAAATTGT GTAAA-5'
	                                                TCCCCTCTTTTATTGT
	                                                    Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 001-050482666
Forward Primer: 5'-CTGCTGTCTGATTTGGCTACTGC-3' T_m: 59.148
Reverse Primer: 5'-CCCAAACGAACCGTACCAAAAGAG-3' T_m: 59.038
Expected PCR Product Length: 356
Expected CelI Cleavage Product Sizes: 133, 223
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr1:50482773-50482820

Rank: 78	Homology Score: 2	TALEN Score: 49.35
	Genomic Region: Intergenic	Closest Gene: lifra
Match Type: L13L
	           Left Nuclease
	         TGTTATTTTCTCCCCT
	5'-GCCCC TGTTTATTTTTCCCCC     AATTTCTGTTTAA     AGGAGAGAAAAAAAAT TCAAC-3'
	3'-CGGGG ACAAATAAAAAGGGGG     TTAAAGACAAATT     TCCTCTCTTTTTTTTA AGTTG-5'
	                                                TCCCCTCTTTTATTGT
	                                                    Left Nuclease
Warning! Primer pair was generated using suboptimal parameters. 
Primer Index: 005-007749854
Forward Primer: 5'-GTGAACCTTGATAAGTGCTGAGCG-3' T_m: 59.038
Reverse Primer: 5'-CCTAGTTAAGCCTTTAAATGTCGCTTTAAGCTG-3' T_m: 59.758
Expected PCR Product Length: 381
Expected CelI Cleavage Product Sizes: 208, 173
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr5:7750038-7750082

Rank: 79	Homology Score: 2	TALEN Score: 49.31
	Genomic Region: Intron	Closest Gene: magi2
Match Type: R15L
	          Right Nuclease
	         TCCACCAGCTCAAAAAT
	5'-TCATT TCCACCATTCGAAAAAT   GAAAAACTAAATGAA    AGTGGCAAAAATAAAA GAGAC-3'
	3'-AGTAA AGGTGGTAAGCTTTTTA   CTTTTTGATTTACTT    TCACCGTTTTTATTTT CTCTG-5'
	                                                TCCCCTCTTTTATTGT
	                                                    Left Nuclease
Warning! Primer pair was generated using suboptimal parameters. 
Primer Index: 004-021822817
Forward Primer: 5'-CCTTGCTCTGTTGCTCTAAACATCATTTAGGA-3' T_m: 59.848
Reverse Primer: 5'-CTTGACAGAAAAGCTGTGGCCTAG-3' T_m: 59.038
Expected PCR Product Length: 271
Expected CelI Cleavage Product Sizes: 139, 132
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr4:21822930-21822977

Rank: 80	Homology Score: 2	TALEN Score: 49.28
	Genomic Region: Intron	Closest Gene: si:dkey-11f4.16
Match Type: R13L
	          Right Nuclease
	         TCCACCAGCTCAAAAAT
	5'-ACTCA TTCACAAGCTGAAAAAA    ATTACAAACTACC     AGGAGAGAAAAACAAA GAGAA-3'
	3'-TGAGT AAGTGTTCGACTTTTTT    TAATGTTTGATGG     TCCTCTCTTTTTGTTT CTCTT-5'
	                                                TCCCCTCTTTTATTGT
	                                                    Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 009-000021675
Forward Primer: 5'-TTATCGGGGGTCGCCACAGT-3' T_m: 59.508
Reverse Primer: 5'-GCCTTGGTGCAAATATTACAGCCG-3' T_m: 59.038
Expected PCR Product Length: 298
Expected CelI Cleavage Product Sizes: 137, 161
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr9:21787-21832

Rank: 81	Homology Score: 2	TALEN Score: 49.26
	Genomic Region: Intergenic	Closest Gene: brd3a
Match Type: R13L
	          Right Nuclease
	         TCCACCAGCTCAAAAAT
	5'-AAATC AAAACCAGTTCAAAAAT    CCCACCACCTAAT     AAGGGATAAAAACACA CTCAC-3'
	3'-TTTAG TTTTGGTCAAGTTTTTA    GGGTGGTGGATTA     TTCCCTATTTTTGTGT GAGTG-5'
	                                                TCCCCTCTTTTATTGT
	                                                    Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 021-017236818
Forward Primer: 5'-GGAAGCAGGGGACATTCAACAAAG-3' T_m: 59.038
Reverse Primer: 5'-GCACACTACACAGGGCATATGG-3' T_m: 59.258
Expected PCR Product Length: 350
Expected CelI Cleavage Product Sizes: 218, 132
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr21:17237011-17237056

Rank: 82	Homology Score: 2	TALEN Score: 49.26
	Genomic Region: Intergenic	Closest Gene: smpd4
Match Type: R13L
	          Right Nuclease
	         TCCACCAGCTCAAAAAT
	5'-AAATC AAAACCAGTTCAAAAAT    CCCACCACCTAAT     AAGGGATAAAAACACA CTCAC-3'
	3'-TTTAG TTTTGGTCAAGTTTTTA    GGGTGGTGGATTA     TTCCCTATTTTTGTGT GAGTG-5'
	                                                TCCCCTCTTTTATTGT
	                                                    Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 021-017452153
Forward Primer: 5'-GGAAGCAGGGGACATTCAACAAAG-3' T_m: 59.038
Reverse Primer: 5'-GCACACTACACAGGGCATATGG-3' T_m: 59.258
Expected PCR Product Length: 349
Expected CelI Cleavage Product Sizes: 217, 132
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr21:17452345-17452390

Rank: 83	Homology Score: 2	TALEN Score: 49.22
	Genomic Region: Intergenic	Closest Gene: fstl1b
Match Type: L19R
	           Left Nuclease
	         TGTTATTTTCTCCCCT
	5'-TAAAG GTTTTTTTTTTCCCCT  TCACCTCGTCAATTGCTAA ACTTTTGGACTTGTGGA ACTGC-3'
	3'-ATTTC CAAAAAAAAAAGGGGA  AGTGGAGCAGTTAACGATT TGAAAACCTGAACACCT TGACG-5'
	                                               TAAAAACTCGACCACCT
	                                                   Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 009-009947600
Forward Primer: 5'-ATGATGGACGTCCAGGTTCTCCAG-3' T_m: 60.908
Reverse Primer: 5'-GGTGACAGGTCCAGACAGATATC-3' T_m: 59.148
Expected PCR Product Length: 392
Expected CelI Cleavage Product Sizes: 131, 261
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr9:9947703-9947754

Rank: 84	Homology Score: 2	TALEN Score: 49.19
	Genomic Region: Exon	Closest Gene: dmd
Match Type: L17R
	           Left Nuclease
	         TGTTATTTTCTCCCCT
	5'-TTGAG TCTCTTTTTCTCACCT   TGGCTGTGAGGGAAGGA  GTTGTTGAGCTGCTCGA TGACG-3'
	3'-AACTC AGAGAAAAAGAGTGGA   ACCGACACTCCCTTCCT  CAACAACTCGACGAGCT ACTGC-5'
	                                               TAAAAACTCGACCACCT
	                                                   Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 001-010011820
Forward Primer: 5'-CCATGGGAGCTCACTGTCCT-3' T_m: 59.508
Reverse Primer: 5'-GCAGGACAACTTCCAGTGGC-3' T_m: 59.508
Expected PCR Product Length: 432
Expected CelI Cleavage Product Sizes: 283, 149
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr1:10012076-10012125

Rank: 85	Homology Score: 2	TALEN Score: 49.18
	Genomic Region: Intergenic	Closest Gene: zgc:153153
Match Type: L17R
	           Left Nuclease
	         TGTTATTTTCTCCCCT
	5'-GTTTT AGTTATTTCTTTCCCT   TCGTCCTTTATTTGCAT  ATTTTTAATGTTGTGGA ATTGG-3'
	3'-CAAAA TCAATAAAGAAAGGGA   AGCAGGAAATAAACGTA  TAAAAATTACAACACCT TAACC-5'
	                                               TAAAAACTCGACCACCT
	                                                   Right Nuclease
Warning! Primer pair was generated using suboptimal parameters. 
Primer Index: 021-020150488
Forward Primer: 5'-CTGGCTTAGCTCTCTAGTACTCTC-3' T_m: 59.038
Reverse Primer: 5'-CGTGAGTAGCTTAAAGGCATAGTTCACTCAA-3' T_m: 59.958
Expected PCR Product Length: 450
Expected CelI Cleavage Product Sizes: 195, 255
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr21:20150656-20150705

Rank: 86	Homology Score: 2	TALEN Score: 49.15
	Genomic Region: Intergenic	Closest Gene: ppp1r13ba
Match Type: L16R
	           Left Nuclease
	         TGTTATTTTCTCCCCT
	5'-GGTTA AGTTATTTTTTGCCTT   TTTACTTCATTTGCAT   ACTTTTGATGTGGTGAA ATTAG-3'
	3'-CCAAT TCAATAAAAAACGGAA   AAATGAAGTAAACGTA   TGAAAACTACACCACTT TAATC-5'
	                                               TAAAAACTCGACCACCT
	                                                   Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 017-014447063
Forward Primer: 5'-GATAGGTGACAAGACTTTTGTCAGGTAGTG-3' T_m: 60.058
Reverse Primer: 5'-CATCTGAGGAGAAATGGTCTGTGC-3' T_m: 59.038
Expected PCR Product Length: 396
Expected CelI Cleavage Product Sizes: 188, 208
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr17:14447225-14447273

Rank: 87	Homology Score: 2	TALEN Score: 49.08
	Genomic Region: Intron	Closest Gene: gpd2
Match Type: L15L
	           Left Nuclease
	         TGTTATTTTCTCCCCT
	5'-TGCTG TGTTATTTGCTAAGCT    CCTGTATTTAAAAAA    AGAAGGGAAAAAAACA GCCAG-3'
	3'-ACGAC ACAATAAACGATTCGA    GGACATAAATTTTTT    TCTTCCCTTTTTTTGT CGGTC-5'
	                                                TCCCCTCTTTTATTGT
	                                                    Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 006-012302933
Forward Primer: 5'-TCTGTATATACGGCATGTATAGCACTGGAG-3' T_m: 60.058
Reverse Primer: 5'-TCCCCCCAAAAAGTAACTAGTCGC-3' T_m: 59.038
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 134, 136
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr6:12303042-12303088

Rank: 88	Homology Score: 2	TALEN Score: 49.05
	Genomic Region: Intergenic	Closest Gene: nr2f2
Match Type: L13L
	           Left Nuclease
	         TGTTATTTTCTCCCCT
	5'-CCCAC TGTTTATTTTTTCCCT     TCAATTTCTGTTT     AATGGAGAAAAAAAAA AATTC-3'
	3'-GGGTG ACAAATAAAAAAGGGA     AGTTAAAGACAAA     TTACCTCTTTTTTTTT TTAAG-5'
	                                                TCCCCTCTTTTATTGT
	                                                    Left Nuclease
Warning! Primer pair was generated using suboptimal parameters. 
Primer Index: 018-023875023
Forward Primer: 5'-CATTCTAGCCGGAAAAAAAAAAAAATTTTCTCCAGAGG-3' T_m: 59.358
Reverse Primer: 5'-CCTTAAGATGTATAGAAGTGTCGTGAAAATATCTAGTG-3' T_m: 59.358
Expected PCR Product Length: 428
Expected CelI Cleavage Product Sizes: 276, 152
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr18:23875275-23875319

Rank: 89	Homology Score: 2	TALEN Score: 49.04
	Genomic Region: Intergenic	Closest Gene: zgc:195356
Match Type: R18R
	          Right Nuclease
	         TCCACCAGCTCAAAAAT
	5'-TGCTG TCCAACAGCTGCAATAT TTGTCAAACTGTAGTGAG  TTTATTGATCTGCTGGA GTAAT-3'
	3'-ACGAC AGGTTGTCGACGTTATA AACAGTTTGACATCACTC  AAATAACTAGACGACCT CATTA-5'
	                                               TAAAAACTCGACCACCT
	                                                   Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 007-038203459
Forward Primer: 5'-GGATGCAGATGCTCAGTATCTCAG-3' T_m: 59.038
Reverse Primer: 5'-CTGTTCTTGTGAGCAAATGCGGC-3' T_m: 59.148
Expected PCR Product Length: 444
Expected CelI Cleavage Product Sizes: 189, 255
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr7:38203620-38203671

Rank: 90	Homology Score: 2	TALEN Score: 49.03
	Genomic Region: Intergenic	Closest Gene: pcdh19
Match Type: L13L
	           Left Nuclease
	         TGTTATTTTCTCCCCT
	5'-TTGAA TGTTTTTTCCTTCCGT     TAAACAGAAATTG     GGGGGAAAAATAAACA GGAGG-3'
	3'-AACTT ACAAAAAAGGAAGGCA     ATTTGTCTTTAAC     CCCCCTTTTTATTTGT CCTCC-5'
	                                                TCCCCTCTTTTATTGT
	                                                    Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 014-041273544
Forward Primer: 5'-CCTGCCTAGTTACCCCAATTTGC-3' T_m: 59.148
Reverse Primer: 5'-GTGTTTCCATTACAGATTGTACAGCGGTTC-3' T_m: 60.058
Expected PCR Product Length: 345
Expected CelI Cleavage Product Sizes: 195, 150
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr14:41273715-41273759

Rank: 91	Homology Score: 2	TALEN Score: 49.02
	Genomic Region: Intergenic	Closest Gene: epha4a
Match Type: R14L
	          Right Nuclease
	         TCCACCAGCTCAAAAAT
	5'-TTTCT TGCACAATCTCATAAAT   ACTTATCACCAGAC     AGAGGAGAAATTAAGC CGACT-3'
	3'-AAAGA ACGTGTTAGAGTATTTA   TGAATAGTGGTCTG     TCTCCTCTTTAATTCG GCTGA-5'
	                                                TCCCCTCTTTTATTGT
	                                                    Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 002-039762596
Forward Primer: 5'-GAAAAGGTTCCAGGGCTTTGTTGC-3' T_m: 59.038
Reverse Primer: 5'-CATTTCCCATAGCGCCGACAAC-3' T_m: 59.258
Expected PCR Product Length: 285
Expected CelI Cleavage Product Sizes: 154, 131
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr2:39762725-39762771

Rank: 92	Homology Score: 2	TALEN Score: 48.98
	Genomic Region: Intergenic	Closest Gene: N/A
Match Type: R18L
	          Right Nuclease
	         TCCACCAGCTCAAAAAT
	5'-CACTG TCGAGCAGCTCCAATAT TAAATCACAGCACATTCC   TGGGGAAAAAAAAAAA AAGTG-3'
	3'-GTGAC AGCTCGTCGAGGTTATA ATTTAGTGTCGTGTAAGG   ACCCCTTTTTTTTTTT TTCAC-5'
	                                                TCCCCTCTTTTATTGT
	                                                    Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 163-000017005
Forward Primer: 5'-CGGTGTATAATGCTGGTGTAGCTC-3' T_m: 59.038
Reverse Primer: 5'-GCACCCTTTCTGTTGACTAAAGCC-3' T_m: 59.038
Expected PCR Product Length: 313
Expected CelI Cleavage Product Sizes: 144, 169
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - Zv9_NA163:17122-17172

Rank: 93	Homology Score: 2	TALEN Score: 48.95
	Genomic Region: Intron	Closest Gene: msi2b
Match Type: L18L
	           Left Nuclease
	         TGTTATTTTCTCCCCT
	5'-TCTCT CGTTATTGTCTCCTTT  CAGCAAGAGGTTTCTTTC   AGGAGAAAAAACAAAA CATTC-3'
	3'-AGAGA GCAATAACAGAGGAAA  GTCGTTCTCCAAAGAAAG   TCCTCTTTTTTGTTTT GTAAG-5'
	                                                TCCCCTCTTTTATTGT
	                                                    Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 015-029982775
Forward Primer: 5'-GGCAGAGCCAAATTACAGTCCATC-3' T_m: 59.038
Reverse Primer: 5'-TTACGTGGCTGCCGTTTCTGC-3' T_m: 59.378
Expected PCR Product Length: 273
Expected CelI Cleavage Product Sizes: 143, 130
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr15:29982891-29982940

Rank: 94	Homology Score: 2	TALEN Score: 48.87
	Genomic Region: Intergenic	Closest Gene: zic3
Match Type: R16L
	          Right Nuclease
	         TCCACCAGCTCAAAAAT
	5'-AGCTT TTCATCAGTTTAAAAAT  GTCCTTAAAGGCACAC    AAGGCTTAAAATAACA GTTCT-3'
	3'-TCGAA AAGTAGTCAAATTTTTA  CAGGAATTTCCGTGTG    TTCCGAATTTTATTGT CAAGA-5'
	                                                TCCCCTCTTTTATTGT
	                                                    Left Nuclease
Warning! Primer pair was generated using suboptimal parameters. 
Primer Index: 014-032838949
Forward Primer: 5'-GTGCGTCTTTACGGACTTGAATATATTTACCAG-3' T_m: 59.758
Reverse Primer: 5'-CACATTTTCTGGGTGTACTTCACATTTGGTTC-3' T_m: 59.848
Expected PCR Product Length: 395
Expected CelI Cleavage Product Sizes: 260, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr14:32839183-32839231

Rank: 95	Homology Score: 2	TALEN Score: 48.84
	Genomic Region: Intergenic	Closest Gene: piwil2
Match Type: L19L
	           Left Nuclease
	         TGTTATTTTCTCCCCT
	5'-GACAC TGTTATTTTCTCGGAA  GAATGCAAAGGTCACTTAG  AGGGGAGAAAGAGAGA GAGAG-3'
	3'-CTGTG ACAATAAAAGAGCCTT  CTTACGTTTCCAGTGAATC  TCCCCTCTTTCTCTCT CTCTC-5'
	                                                TCCCCTCTTTTATTGT
	                                                    Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 005-018181209
Forward Primer: 5'-GGCCACCCTGATCACGATTAAC-3' T_m: 59.258
Reverse Primer: 5'-CAACTTCACACAAGCACATTCCGC-3' T_m: 59.038
Expected PCR Product Length: 292
Expected CelI Cleavage Product Sizes: 143, 149
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr5:18181325-18181375

Rank: 96	Homology Score: 2	TALEN Score: 48.84
	Genomic Region: Intron	Closest Gene: pgm2l1
Match Type: L16L
	           Left Nuclease
	         TGTTATTTTCTCCCCT
	5'-AACAC TTTTTTTTTCTCTCGT   AAATAAGTAAAATAAT    ATTAAAGAAAATAACA TCTGC-3'
	3'-TTGTG AAAAAAAAAGAGAGCA   TTTATTCATTTTATTA    TAATTTCTTTTATTGT AGACG-5'
	                                                TCCCCTCTTTTATTGT
	                                                    Left Nuclease
Warning! Primer pair was generated using suboptimal parameters. 
Primer Index: 015-004995201
Forward Primer: 5'-CTCAACATTTTATCAGGTAATGCAATACCAGTGAC-3' T_m: 59.578
Reverse Primer: 5'-TGCCACGAAAGATTAAGGCCATTCTGC-3' T_m: 60.438
Expected PCR Product Length: 407
Expected CelI Cleavage Product Sizes: 277, 130
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr15:4995452-4995499

Rank: 97	Homology Score: 2	TALEN Score: 48.73
	Genomic Region: Intergenic	Closest Gene: hnf4b
Match Type: L18L
	           Left Nuclease
	         TGTTATTTTCTCCCCT
	5'-TCAGC GGTTATCTTCACCTCT  GGAGGGACACTGACGGGG   AGGGGAAAAAAAACAA TTGCG-3'
	3'-AGTCG CCAATAGAAGTGGAGA  CCTCCCTGTGACTGCCCC   TCCCCTTTTTTTTGTT AACGC-5'
	                                                TCCCCTCTTTTATTGT
	                                                    Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 007-070059039
Forward Primer: 5'-GGTGTTGGAGGCTGCTTTTAGG-3' T_m: 59.258
Reverse Primer: 5'-GCAAGAAATTCTCCAACCGAGAGG-3' T_m: 59.038
Expected PCR Product Length: 334
Expected CelI Cleavage Product Sizes: 149, 185
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr7:70059161-70059210

Rank: 98	Homology Score: 2	TALEN Score: 48.67
	Genomic Region: Intergenic	Closest Gene: csf1ra
Match Type: L19R
	           Left Nuclease
	         TGTTATTTTCTCCCCT
	5'-AGCTT TGTTCTTCTCTGCTCT  TAGTTAGTGTGACTGGAGG ATAGTTGAGCTGCTGGT GTATT-3'
	3'-TCGAA ACAAGAAGAGACGAGA  ATCAATCACACTGACCTCC TATCAACTCGACGACCA CATAA-5'
	                                               TAAAAACTCGACCACCT
	                                                   Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 015-043743524
Forward Primer: 5'-GCTCATGGTGGATGTGAATGTCTG-3' T_m: 59.038
Reverse Primer: 5'-TGCTAACCACTGTGTCACCCC-3' T_m: 59.378
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 140, 130
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr15:43743636-43743687

Rank: 99	Homology Score: 2	TALEN Score: 48.67
	Genomic Region: Intron	Closest Gene: zgc:112142
Match Type: L14L
	           Left Nuclease
	         TGTTATTTTCTCCCCT
	5'-CCCCC TGTTTATTTTTTCCCT    CAACTTCTGTTTAA     AGGAGAGAAAAAAAAT TTCAA-3'
	3'-GGGGG ACAAATAAAAAAGGGA    GTTGAAGACAAATT     TCCTCTCTTTTTTTTA AAGTT-5'
	                                                TCCCCTCTTTTATTGT
	                                                    Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 007-017155191
Forward Primer: 5'-GGAGAGCAATGGTACTTCATTAGCAGC-3' T_m: 60.438
Reverse Primer: 5'-CTTGCCTAATTACGCTAACCTGCC-3' T_m: 59.038
Expected PCR Product Length: 440
Expected CelI Cleavage Product Sizes: 226, 214
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr7:17155392-17155437

Rank: 100	Homology Score: 2	TALEN Score: 48.67
	Genomic Region: Intron	Closest Gene: cntfr
Match Type: L17L
	           Left Nuclease
	         TGTTATTTTCTCCCCT
	5'-TAAAA TGTTAATATTTACCCT   CCTAAAAGTTCAGAAAC   AGCAAAAAAAATAACA GGTAA-3'
	3'-ATTTT ACAATTATAAATGGGA   GGATTTTCAAGTCTTTG   TCGTTTTTTTTATTGT CCATT-5'
	                                                TCCCCTCTTTTATTGT
	                                                    Left Nuclease
Warning! Primer pair was generated using suboptimal parameters. 
Primer Index: 010-013532358
Forward Primer: 5'-CAAAGGGCTCACGGTTTGTCTTGT-3' T_m: 59.038
Reverse Primer: 5'-GGTTTTGTTTGCTGTTTCTTAAGTTTGAAGGGG-3' T_m: 59.758
Expected PCR Product Length: 445
Expected CelI Cleavage Product Sizes: 293, 152
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr10:13532625-13532673

Rank: 101	Homology Score: 2	TALEN Score: 48.35
	Genomic Region: Intergenic	Closest Gene: hs3st2
Match Type: L17L
	           Left Nuclease
	         TGTTATTTTCTCCCCT
	5'-CATGC TGGTATTTCTTCCCTT   TGGCTTTAGGTTTTGGA   AAGGGAAAAAAAAACG CCACC-3'
	3'-GTACG ACCATAAAGAAGGGAA   ACCGAAATCCAAAACCT   TTCCCTTTTTTTTTGC GGTGG-5'
	                                                TCCCCTCTTTTATTGT
	                                                    Left Nuclease
Warning! Primer pair was generated using suboptimal parameters. 
Primer Index: 003-045064753
Forward Primer: 5'-CGTTCATTGTTAGTTCATGTTAACTCATGGTGC-3' T_m: 59.758
Reverse Primer: 5'-CACGGGAGGTCTGTCAAGC-3' T_m: 59.658
Expected PCR Product Length: 445
Expected CelI Cleavage Product Sizes: 313, 132
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr3:45065040-45065088

Rank: 102	Homology Score: 2	TALEN Score: 48.34
	Genomic Region: Intergenic	Closest Gene: zgc:194737
Match Type: L16R
	           Left Nuclease
	         TGTTATTTTCTCCCCT
	5'-TTGTA TGTTTTTTTTTCCTTT   TAAAGCAGAGAATATT   ATTTATGAGCTTGTTGG GGGAT-3'
	3'-AACAT ACAAAAAAAAAGGAAA   ATTTCGTCTCTTATAA   TAAATACTCGAACAACC CCCTA-5'
	                                               TAAAAACTCGACCACCT
	                                                   Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 015-014303624
Forward Primer: 5'-CGCATGACCGCGTATCGATACA-3' T_m: 59.258
Reverse Primer: 5'-CTGCTCCCCTGAAACAGTCAAC-3' T_m: 59.258
Expected PCR Product Length: 390
Expected CelI Cleavage Product Sizes: 212, 178
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr15:14303810-14303858

Rank: 103	Homology Score: 2	TALEN Score: 48.24
	Genomic Region: Intron	Closest Gene: scn8ab
Match Type: L13L
	           Left Nuclease
	         TGTTATTTTCTCCCCT
	5'-TGTCA AGTTATTTTCATCGCT     TTCAATGAACAAC     AGGGAACAAAATAAGG GCCTA-3'
	3'-ACAGT TCAATAAAAGTAGCGA     AAGTTACTTGTTG     TCCCTTGTTTTATTCC CGGAT-5'
	                                                TCCCCTCTTTTATTGT
	                                                    Left Nuclease
No Primers
Primer Index: No Primers
Forward Primer: 5'-No Primers-3' T_m: No Primers
Reverse Primer: 5'-No Primers-3' T_m: No Primers
Expected PCR Product Length: No Primers
Expected CelI Cleavage Product Sizes: No Primers, No Primers
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr6:39339584-39339628

Rank: 104	Homology Score: 2	TALEN Score: 48.24
	Genomic Region: Intergenic	Closest Gene: aqp3b
Match Type: L15L
	           Left Nuclease
	         TGTTATTTTCTCCCCT
	5'-CTATT GGTTATTATCTTCTCT    TCGTAAACAGAAATT    AGGGAAAAAATAAACA GGGGG-3'
	3'-GATAA CCAATAATAGAAGAGA    AGCATTTGTCTTTAA    TCCCTTTTTTATTTGT CCCCC-5'
	                                                TCCCCTCTTTTATTGT
	                                                    Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 021-015439457
Forward Primer: 5'-CGCAACACAATAACTTGCCTAAACTGCC-3' T_m: 60.298
Reverse Primer: 5'-GGCTGTACGAGTTCTGACATTGC-3' T_m: 59.148
Expected PCR Product Length: 347
Expected CelI Cleavage Product Sizes: 179, 168
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr21:15439611-15439657

Rank: 105	Homology Score: 2	TALEN Score: 48.01
	Genomic Region: Intergenic	Closest Gene: wnt9b
Match Type: R15L
	          Right Nuclease
	         TCCACCAGCTCAAAAAT
	5'-ACGGT GCCACCAGCTTTACAAT   GAAAGCCAATGTGAA    AGGCATGAAAATAAAA ATGGA-3'
	3'-TGCCA CGGTGGTCGAAATGTTA   CTTTCGGTTACACTT    TCCGTACTTTTATTTT TACCT-5'
	                                                TCCCCTCTTTTATTGT
	                                                    Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 003-037596249
Forward Primer: 5'-CCATTGCGGACGTGTCACTG-3' T_m: 59.508
Reverse Primer: 5'-CACGTCGAAAAATCGGCAAGACTG-3' T_m: 59.038
Expected PCR Product Length: 285
Expected CelI Cleavage Product Sizes: 155, 130
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr3:37596378-37596425

Rank: 106	Homology Score: 2	TALEN Score: 47.94
	Genomic Region: Intergenic	Closest Gene: vegfc
Match Type: L14L
	           Left Nuclease
	         TGTTATTTTCTCCCCT
	5'-TCTTT TCTTTTTTTCTCTGCT    AACCAACTAAGGCT     AGGCGATAAAATCACT ATCTT-3'
	3'-AGAAA AGAAAAAAAGAGACGA    TTGGTTGATTCCGA     TCCGCTATTTTAGTGA TAGAA-5'
	                                                TCCCCTCTTTTATTGT
	                                                    Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 001-039497098
Forward Primer: 5'-TTGAGGCAAGTTGGTGCAAAACCC-3' T_m: 59.038
Reverse Primer: 5'-CCACGTCACAAGTCTCCATTGGAA-3' T_m: 59.038
Expected PCR Product Length: 333
Expected CelI Cleavage Product Sizes: 131, 202
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr1:39497204-39497249

Plate format Oligo Order Form
Well Name Sequence 5' mods Internal Notes
Primer Plate #1
A1 024-027401962F GCTGAGGGGAACACCAAACATG Huang_tnikb_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
A2 024-027401962R CCAGTATTGCAGCCCTTGTAATACACTGTT Huang_tnikb_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
B1 016-044051024F GTGCTTAATGCACAGCGGCATC Huang_tnikb_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
B2 016-044051024R CAACCCTCACCCTAGTCTACC Huang_tnikb_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
C1 008-026575347F GTCTTAAGGCCTTTCGATTGCAATGCAAAC Huang_tnikb_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
C2 008-026575347R GTGTGGGTGTTACCCAGCACT Huang_tnikb_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
D1 016-047429150F CACTCCTCAACTATAGGGGTGG Huang_tnikb_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
D2 016-047429150R CACTGATGCCTGTCGACGACT Huang_tnikb_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
E1 002-015693319F GCAACCGGGTAACCACACTTC Huang_tnikb_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
E2 002-015693319R GGAGACTGCAAAGAAACAAAGGCC Huang_tnikb_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
F1 003-041144063F CTGTGAATTGTGTGGAACCCTGC Huang_tnikb_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
F2 003-041144063R GGCAAAAGGCCAATAAAGTGTCCG Huang_tnikb_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
G1 007-047515148F GCACAGATCGTCTTATCCTCTCTC Huang_tnikb_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
G2 007-047515148R GGTCACTACTGCACAGGTACATG Huang_tnikb_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
H1 002-046852941F GTGACCCCTGATGAATAAAGGGAC Huang_tnikb_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
H2 002-046852941R CAAAGGCTACCAAGGGTAACGC Huang_tnikb_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
A3 013-042252335F CGTTGCCTGGTTTGATGATTCTCG Huang_tnikb_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
A4 013-042252335R GGGAGAAGTCGTGATTATGGGG Huang_tnikb_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
B3 024-019588664F GCTCAGACGTTCCACAATCCAACA Huang_tnikb_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
B4 024-019588664R GTGTTGGGACAACATGAAGCAATTATGTGG Huang_tnikb_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
C3 No PrimersF No Primers Huang_tnikb_Ranking-TALENv2.0	No Primers
C4 No PrimersR No Primers Huang_tnikb_Ranking-TALENv2.0	No Primers
D3 No PrimersF No Primers Huang_tnikb_Ranking-TALENv2.0	No Primers
D4 No PrimersR No Primers Huang_tnikb_Ranking-TALENv2.0	No Primers
E3 021-017765608F GGCTAGCTCAGCTAACGCGT Huang_tnikb_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
E4 021-017765608R CAAGCGACCTAGCACTTCTAGAC Huang_tnikb_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
F3 023-004000336F GCGCAAGATTCTGACAAGCTTTCG Huang_tnikb_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
F4 023-004000336R GCATGCGCTTGACGCAGAAGT Huang_tnikb_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
G3 017-017427769F GTTTCGCCTCTCCTTTGAGGAC Huang_tnikb_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
G4 017-017427769R GCAGGTTGAGAGTGGGTGGT Huang_tnikb_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
H3 017-014089806F CTCTGTTATTTTCCCTCTCTGCCC Huang_tnikb_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
H4 017-014089806R CCCACCTGCCCCTGAAATCT Huang_tnikb_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
A5 014-004719194F AGCATGTTGAAATCAATAAATAGCCTACTACTGTCC Huang_tnikb_Ranking-TALENv2.0	Warning! Primer pair was generated using suboptimal parameters. 
A6 014-004719194R GCTGAGGCGCTGGACAAAAAC Huang_tnikb_Ranking-TALENv2.0	Warning! Primer pair was generated using suboptimal parameters. 
B5 025-030215268F GCATTGTGGCAACAAGCTTTCGAC Huang_tnikb_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
B6 025-030215268R CTTATGTGTATCTGTGGCCAAAGCTCAATG Huang_tnikb_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
C5 023-015609480F GTTTGACGGACAGTGTTTAGGAGG Huang_tnikb_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
C6 023-015609480R CGAGAGGGGGTATAAGGACAG Huang_tnikb_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
D5 011-006696390F CTCTTCAGAGCTCTAGCAGGATTC Huang_tnikb_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
D6 011-006696390R CACATGCACGCAGTTCTGTAGAG Huang_tnikb_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
E5 022-010953992F GTATTTTGGTCTTAGTAGTGCTTTGAAATGCGC Huang_tnikb_Ranking-TALENv2.0	Warning! Primer pair was generated using suboptimal parameters. 
E6 022-010953992R GCCATGCCCCCTTAAAGATGC Huang_tnikb_Ranking-TALENv2.0	Warning! Primer pair was generated using suboptimal parameters. 
F5 020-037794081F GACGTATGGGAGATCTGCCCT Huang_tnikb_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
F6 020-037794081R GCTTGGTCATTTGTTTTGGGTGAACTATCC Huang_tnikb_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
G5 017-004427364F GGAGTCCGCAGAGTTTAACTGG Huang_tnikb_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
G6 017-004427364R GAAGTTTCCTGCTCGAGGAGCT Huang_tnikb_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
H5 002-008682501F CTACGGGCACTTTTATGTCCTTTGATTTCC Huang_tnikb_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
H6 002-008682501R GGAAATGACACTGATACTGTGTGACAGC Huang_tnikb_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
A7 012-030852351F TTGGACTGTGGGGGAAACCCAT Huang_tnikb_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
A8 012-030852351R GTCTTCAGTGTCACATGATCCCTC Huang_tnikb_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
B7 007-058097761F CTCTAACCTGCCTAGGTAACCC Huang_tnikb_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
B8 007-058097761R GAATGGGCAGATGAACTGATATGTCAACAC Huang_tnikb_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
C7 016-028686098F TGACGTATCCCGGGACTGC Huang_tnikb_Ranking-TALENv2.0	Warning! Primer pair was generated using suboptimal parameters. 
C8 016-028686098R GGGGGACAATTCATATTCATAAAATCCAAGCC Huang_tnikb_Ranking-TALENv2.0	Warning! Primer pair was generated using suboptimal parameters. 
D7 007-024009350F GGATTCTTGCTCCAAAGATCCCAG Huang_tnikb_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
D8 007-024009350R GCGATTACCACAGTGGAATGAACC Huang_tnikb_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
E7 006-028310006F GTGTCGGTGTGAACTTCCAGTAG Huang_tnikb_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
E8 006-028310006R CGAAAACAAGCTCAAGGCCACC Huang_tnikb_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
F7 011-034935667F CCTGTGTATGGGTAGCCTTCC Huang_tnikb_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
F8 011-034935667R GCAGTATCTCAGTACGTTTTCCTTGTGCT Huang_tnikb_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
G7 485-000132960F GCCTTTCTACACCTCCCTTTCTTC Huang_tnikb_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
G8 485-000132960R CTCTCTTCTCACCAGCAGAAGC Huang_tnikb_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
H7 019-006276406F GATGGGACAGTATGGACGCG Huang_tnikb_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
H8 019-006276406R GACGTCCACAGCAGGGAATTTG Huang_tnikb_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
A9 020-001352095F GTGAGCCGGATGCAAGTGTC Huang_tnikb_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
A10 020-001352095R TGTCCAGTTTAAACCAGGCTGGTC Huang_tnikb_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
B9 023-029796044F ACGACGGCAAGTAAGTGGTCC Huang_tnikb_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
B10 023-029796044R CTCAGTCCTGAAGAGGTAAGGC Huang_tnikb_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
C9 013-032722528F TGGACTGATTACACACACATGGCC Huang_tnikb_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
C10 013-032722528R TTGCGGTTGGCTCTGTTTTAGTGC Huang_tnikb_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
D9 009-014999443F CTTCATGGAATGCCGGATGCC Huang_tnikb_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
D10 009-014999443R GGCCCATGTAGTCTCAGGATAAG Huang_tnikb_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
E9 011-033515570F CAGAATTTGTGTTTGCAGAACATTTGCATGAGG Huang_tnikb_Ranking-TALENv2.0	Warning! Primer pair was generated using suboptimal parameters. 
E10 011-033515570R GGTGCCCAAAAATCTCCATATAGTTTTTCCACA Huang_tnikb_Ranking-TALENv2.0	Warning! Primer pair was generated using suboptimal parameters. 
F9 005-037597563F CTTGCCTGTCTTGCCTTGTTAACC Huang_tnikb_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
F10 005-037597563R CACAGAAACGCCAACCGACC Huang_tnikb_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
G9 005-058179155F CAAGCAGTCATTCTGACCACTCTC Huang_tnikb_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
G10 005-058179155R CCACAAACGAAGTGCTACAGGG Huang_tnikb_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
H9 007-027053585F CGAGCTGACATATCAGCGATCTCT Huang_tnikb_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
H10 007-027053585R GGCTAAATGCCTTCTGTCTCCATC Huang_tnikb_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
A11 018-025634847F GAGACTAAAAGAGAAGAGCTGGTAAAACAGG Huang_tnikb_Ranking-TALENv2.0	Warning! Primer pair was generated using suboptimal parameters. 
A12 018-025634847R CTCCCCAGCTTCGCATATGTTTC Huang_tnikb_Ranking-TALENv2.0	Warning! Primer pair was generated using suboptimal parameters. 
B11 011-007452947F GGTGCATAGGATGGGAATCATCTG Huang_tnikb_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
B12 011-007452947R GAGTGGGTGTTGAGAGTGTTGATC Huang_tnikb_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
C11 004-023758407F GCCCTAACTTGCCTAGTTGACC Huang_tnikb_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
C12 004-023758407R GCTAAGGCTTAGAACGTGAACACAAGCA Huang_tnikb_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
D11 004-024096464F GCTAAGGCTTAGAACGTGAACACAAGCA Huang_tnikb_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
D12 004-024096464R GCCCTAACTTGCCTAGTTGACC Huang_tnikb_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
E11 005-008593040F GTGGACGCGGATCGTTTTCATTC Huang_tnikb_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
E12 005-008593040R GCTTTAATCTTGTTCTGGGCCTCC Huang_tnikb_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
F11 No PrimersF No Primers Huang_tnikb_Ranking-TALENv2.0	No Primers
F12 No PrimersR No Primers Huang_tnikb_Ranking-TALENv2.0	No Primers
G11 011-042909323F GCAATTAGAGGGATACACACAGGG Huang_tnikb_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
G12 011-042909323R CCCAATTACCCTATCCTGCCTAGT Huang_tnikb_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
H11 296-000012696F CTCTGAAAACTGGACTGACACGG Huang_tnikb_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
H12 296-000012696R ACTCTTTCCACACTGAGGGCAGG Huang_tnikb_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
Primer Plate #2
A1 010-002176503F CTGCCTAGTTAACCTAGTTACGCC Huang_tnikb_Ranking-TALENv2.0	Warning! Primer pair was generated using suboptimal parameters. 
A2 010-002176503R GCAATGCTGTTCTTTTGAAGCTGCTATTCATC Huang_tnikb_Ranking-TALENv2.0	Warning! Primer pair was generated using suboptimal parameters. 
B1 No PrimersF No Primers Huang_tnikb_Ranking-TALENv2.0	No Primers
B2 No PrimersR No Primers Huang_tnikb_Ranking-TALENv2.0	No Primers
C1 007-031277548F GGAGTGCACAGTGTTCTCACTTG Huang_tnikb_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
C2 007-031277548R CACGCACGCACACAACACAAC Huang_tnikb_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
D1 006-025723296F GAGGGCATGGCTAAGTACAAACTG Huang_tnikb_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
D2 006-025723296R GTGCCAATCCCCGTATTAGATGC Huang_tnikb_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
E1 001-039182385F GCCTTTAAATGTCACTTTAAGCTGTATAGAAGTGTC Huang_tnikb_Ranking-TALENv2.0	Warning! Primer pair was generated using suboptimal parameters. Warning! The sequence of this region of the genome is poorly mapped. 
E2 001-039182385R GGTGGGGGGTTATAGTTTTGTAGC Huang_tnikb_Ranking-TALENv2.0	Warning! Primer pair was generated using suboptimal parameters. Warning! The sequence of this region of the genome is poorly mapped. 
F1 008-028609709F CCGACACTGGGTATTCGTGTAG Huang_tnikb_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
F2 008-028609709R GCCTTATTATCCTAACCTGCCAAGTTAACC Huang_tnikb_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
G1 001-050760345F CTCGTCGGCAGAGAATAAGGTG Huang_tnikb_Ranking-TALENv2.0	Warning! Primer pair was generated using suboptimal parameters. 
G2 001-050760345R GTCATATGTGCCATCATTGGTTTGAACTTAGC Huang_tnikb_Ranking-TALENv2.0	Warning! Primer pair was generated using suboptimal parameters. 
H1 004-034644828F CTTTCCACACTGAGGGCAGG Huang_tnikb_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
H2 004-034644828R CTGAAAACTGGGCTGACACGG Huang_tnikb_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
A3 004-057253541F GCTTGCCAAACTCTTTCCACACTG Huang_tnikb_Ranking-TALENv2.0	Warning! Primer pair was generated using suboptimal parameters. 
A4 004-057253541R CTATACCTTCTATGTTAAGCGGCTTTGACAC Huang_tnikb_Ranking-TALENv2.0	Warning! Primer pair was generated using suboptimal parameters. 
B3 001-019043719F CGCAAGAAGGGGCGAATTTAACC Huang_tnikb_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
B4 001-019043719R CCGCCATTTGGGCCAAATTTTCC Huang_tnikb_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
C3 002-056190023F GGTCAAATCCAACTGTTGGCTCATAAAGTG Huang_tnikb_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
C4 002-056190023R CCGGGTGACCAGGGACTATA Huang_tnikb_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
D3 No PrimersF No Primers Huang_tnikb_Ranking-TALENv2.0	No Primers
D4 No PrimersR No Primers Huang_tnikb_Ranking-TALENv2.0	No Primers
E3 No PrimersF No Primers Huang_tnikb_Ranking-TALENv2.0	No Primers
E4 No PrimersR No Primers Huang_tnikb_Ranking-TALENv2.0	No Primers
F3 020-005773433F CCCTAACCTGCCTAGTTACCC Huang_tnikb_Ranking-TALENv2.0	Primer pair generated using optimal parameters. Warning! The sequence of this region of the genome is poorly mapped. 
F4 020-005773433R GGGGGCTCAACTCCTTATAAGGT Huang_tnikb_Ranking-TALENv2.0	Primer pair generated using optimal parameters. Warning! The sequence of this region of the genome is poorly mapped. 
G3 021-036802701F GACCGCATTTTTCTCCTCCTCAC Huang_tnikb_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
G4 021-036802701R CCGGGTTTGTGCGTTTTAAGTCC Huang_tnikb_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
H3 008-037798987F GCCTAATTACCCTAACCCGCC Huang_tnikb_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
H4 008-037798987R CTGCATGGGGTGGTAGCTTTTC Huang_tnikb_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
A5 019-047559875F CTGGAAGAACGGCTCAAGGTG Huang_tnikb_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
A6 019-047559875R GACTTTAGCTGTTCTCCACATGGTTTGC Huang_tnikb_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
B5 015-019562303F CCTAGAGCTGGTGTCTTGCTTTC Huang_tnikb_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
B6 015-019562303R GGTCCATGCAGGTTCCAATAGG Huang_tnikb_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
C5 005-009640443F CGCAATTCGGACTTTATAACTTGCAATTCTGAC Huang_tnikb_Ranking-TALENv2.0	Warning! Primer pair was generated using suboptimal parameters. 
C6 005-009640443R GTGTTGGGACAAGATGAAAGAATAGTTTGGC Huang_tnikb_Ranking-TALENv2.0	Warning! Primer pair was generated using suboptimal parameters. 
D5 005-009701307F CGCAATTCGGACTTTATAACTTGCAATTCTGAC Huang_tnikb_Ranking-TALENv2.0	Warning! Primer pair was generated using suboptimal parameters. 
D6 005-009701307R GTTGGGACAAGATGAAGGAATAGTTTGGC Huang_tnikb_Ranking-TALENv2.0	Warning! Primer pair was generated using suboptimal parameters. 
E5 022-004365783F GGTGTTTGTGATGGTCAGAGATCC Huang_tnikb_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
E6 022-004365783R GAAGTAACTTGCACAACACTGCATTCAACG Huang_tnikb_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
F5 No PrimersF No Primers Huang_tnikb_Ranking-TALENv2.0	No Primers
F6 No PrimersR No Primers Huang_tnikb_Ranking-TALENv2.0	No Primers
G5 008-028840626F CGTCAAACATCATCAGGGAAACGC Huang_tnikb_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
G6 008-028840626R GCGAGCACACTATACACTATGCAC Huang_tnikb_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
H5 017-007362226F CAGGATCATTGGAATGGTCTAAAACTCAGTG Huang_tnikb_Ranking-TALENv2.0	Warning! Primer pair was generated using suboptimal parameters. 
H6 017-007362226R CCCTTTTCGCAGTGCTGTACAAC Huang_tnikb_Ranking-TALENv2.0	Warning! Primer pair was generated using suboptimal parameters. 
A7 025-002697030F TGTGGCTCAGAGACACACACACAT Huang_tnikb_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
A8 025-002697030R GCGACCCCTGATTAATAAAGGGAC Huang_tnikb_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
B7 003-018061176F GGTCCTCTCCTAACATTCCTTCTG Huang_tnikb_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
B8 003-018061176R GCTGCTGCTGACACATTCAGG Huang_tnikb_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
C7 018-019742010F GAGCAGAAAGAGGGGGAAGATC Huang_tnikb_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
C8 018-019742010R CAATAACTTCCCTAATTACCCTAACCTGCC Huang_tnikb_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
D7 017-017908369F TCTTGCTGCCGGAGCAGAG Huang_tnikb_Ranking-TALENv2.0	Warning! Primer pair was generated using suboptimal parameters. 
D8 017-017908369R GATTCAGTTCAGTCATTTTTAAACCTGGCCC Huang_tnikb_Ranking-TALENv2.0	Warning! Primer pair was generated using suboptimal parameters. 
E7 001-050482666F CTGCTGTCTGATTTGGCTACTGC Huang_tnikb_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
E8 001-050482666R CCCAAACGAACCGTACCAAAAGAG Huang_tnikb_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
F7 005-007749854F GTGAACCTTGATAAGTGCTGAGCG Huang_tnikb_Ranking-TALENv2.0	Warning! Primer pair was generated using suboptimal parameters. 
F8 005-007749854R CCTAGTTAAGCCTTTAAATGTCGCTTTAAGCTG Huang_tnikb_Ranking-TALENv2.0	Warning! Primer pair was generated using suboptimal parameters. 
G7 004-021822817F CCTTGCTCTGTTGCTCTAAACATCATTTAGGA Huang_tnikb_Ranking-TALENv2.0	Warning! Primer pair was generated using suboptimal parameters. 
G8 004-021822817R CTTGACAGAAAAGCTGTGGCCTAG Huang_tnikb_Ranking-TALENv2.0	Warning! Primer pair was generated using suboptimal parameters. 
H7 009-000021675F TTATCGGGGGTCGCCACAGT Huang_tnikb_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
H8 009-000021675R GCCTTGGTGCAAATATTACAGCCG Huang_tnikb_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
A9 021-017236818F GGAAGCAGGGGACATTCAACAAAG Huang_tnikb_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
A10 021-017236818R GCACACTACACAGGGCATATGG Huang_tnikb_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
B9 021-017452153F GGAAGCAGGGGACATTCAACAAAG Huang_tnikb_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
B10 021-017452153R GCACACTACACAGGGCATATGG Huang_tnikb_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
C9 009-009947600F ATGATGGACGTCCAGGTTCTCCAG Huang_tnikb_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
C10 009-009947600R GGTGACAGGTCCAGACAGATATC Huang_tnikb_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
D9 001-010011820F CCATGGGAGCTCACTGTCCT Huang_tnikb_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
D10 001-010011820R GCAGGACAACTTCCAGTGGC Huang_tnikb_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
E9 021-020150488F CTGGCTTAGCTCTCTAGTACTCTC Huang_tnikb_Ranking-TALENv2.0	Warning! Primer pair was generated using suboptimal parameters. 
E10 021-020150488R CGTGAGTAGCTTAAAGGCATAGTTCACTCAA Huang_tnikb_Ranking-TALENv2.0	Warning! Primer pair was generated using suboptimal parameters. 
F9 017-014447063F GATAGGTGACAAGACTTTTGTCAGGTAGTG Huang_tnikb_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
F10 017-014447063R CATCTGAGGAGAAATGGTCTGTGC Huang_tnikb_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
G9 006-012302933F TCTGTATATACGGCATGTATAGCACTGGAG Huang_tnikb_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
G10 006-012302933R TCCCCCCAAAAAGTAACTAGTCGC Huang_tnikb_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
H9 018-023875023F CATTCTAGCCGGAAAAAAAAAAAAATTTTCTCCAGAGG Huang_tnikb_Ranking-TALENv2.0	Warning! Primer pair was generated using suboptimal parameters. 
H10 018-023875023R CCTTAAGATGTATAGAAGTGTCGTGAAAATATCTAGTG Huang_tnikb_Ranking-TALENv2.0	Warning! Primer pair was generated using suboptimal parameters. 
A11 007-038203459F GGATGCAGATGCTCAGTATCTCAG Huang_tnikb_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
A12 007-038203459R CTGTTCTTGTGAGCAAATGCGGC Huang_tnikb_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
B11 014-041273544F CCTGCCTAGTTACCCCAATTTGC Huang_tnikb_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
B12 014-041273544R GTGTTTCCATTACAGATTGTACAGCGGTTC Huang_tnikb_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
C11 002-039762596F GAAAAGGTTCCAGGGCTTTGTTGC Huang_tnikb_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
C12 002-039762596R CATTTCCCATAGCGCCGACAAC Huang_tnikb_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
D11 163-000017005F CGGTGTATAATGCTGGTGTAGCTC Huang_tnikb_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
D12 163-000017005R GCACCCTTTCTGTTGACTAAAGCC Huang_tnikb_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
E11 015-029982775F GGCAGAGCCAAATTACAGTCCATC Huang_tnikb_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
E12 015-029982775R TTACGTGGCTGCCGTTTCTGC Huang_tnikb_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
F11 014-032838949F GTGCGTCTTTACGGACTTGAATATATTTACCAG Huang_tnikb_Ranking-TALENv2.0	Warning! Primer pair was generated using suboptimal parameters. 
F12 014-032838949R CACATTTTCTGGGTGTACTTCACATTTGGTTC Huang_tnikb_Ranking-TALENv2.0	Warning! Primer pair was generated using suboptimal parameters. 
G11 005-018181209F GGCCACCCTGATCACGATTAAC Huang_tnikb_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
G12 005-018181209R CAACTTCACACAAGCACATTCCGC Huang_tnikb_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
H11 015-004995201F CTCAACATTTTATCAGGTAATGCAATACCAGTGAC Huang_tnikb_Ranking-TALENv2.0	Warning! Primer pair was generated using suboptimal parameters. 
H12 015-004995201R TGCCACGAAAGATTAAGGCCATTCTGC Huang_tnikb_Ranking-TALENv2.0	Warning! Primer pair was generated using suboptimal parameters. 
Primer Plate #3
A1 007-070059039F GGTGTTGGAGGCTGCTTTTAGG Huang_tnikb_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
A2 007-070059039R GCAAGAAATTCTCCAACCGAGAGG Huang_tnikb_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
B1 015-043743524F GCTCATGGTGGATGTGAATGTCTG Huang_tnikb_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
B2 015-043743524R TGCTAACCACTGTGTCACCCC Huang_tnikb_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
C1 007-017155191F GGAGAGCAATGGTACTTCATTAGCAGC Huang_tnikb_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
C2 007-017155191R CTTGCCTAATTACGCTAACCTGCC Huang_tnikb_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
D1 010-013532358F CAAAGGGCTCACGGTTTGTCTTGT Huang_tnikb_Ranking-TALENv2.0	Warning! Primer pair was generated using suboptimal parameters. 
D2 010-013532358R GGTTTTGTTTGCTGTTTCTTAAGTTTGAAGGGG Huang_tnikb_Ranking-TALENv2.0	Warning! Primer pair was generated using suboptimal parameters. 
E1 003-045064753F CGTTCATTGTTAGTTCATGTTAACTCATGGTGC Huang_tnikb_Ranking-TALENv2.0	Warning! Primer pair was generated using suboptimal parameters. 
E2 003-045064753R CACGGGAGGTCTGTCAAGC Huang_tnikb_Ranking-TALENv2.0	Warning! Primer pair was generated using suboptimal parameters. 
F1 015-014303624F CGCATGACCGCGTATCGATACA Huang_tnikb_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
F2 015-014303624R CTGCTCCCCTGAAACAGTCAAC Huang_tnikb_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
G1 No PrimersF No Primers Huang_tnikb_Ranking-TALENv2.0	No Primers
G2 No PrimersR No Primers Huang_tnikb_Ranking-TALENv2.0	No Primers
H1 021-015439457F CGCAACACAATAACTTGCCTAAACTGCC Huang_tnikb_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
H2 021-015439457R GGCTGTACGAGTTCTGACATTGC Huang_tnikb_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
A3 003-037596249F CCATTGCGGACGTGTCACTG Huang_tnikb_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
A4 003-037596249R CACGTCGAAAAATCGGCAAGACTG Huang_tnikb_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
B3 001-039497098F TTGAGGCAAGTTGGTGCAAAACCC Huang_tnikb_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
B4 001-039497098R CCACGTCACAAGTCTCCATTGGAA Huang_tnikb_Ranking-TALENv2.0	Primer pair generated using optimal parameters.
106 total sites were located
Mismatch Types	Genomic Regions
0 and 0: 1 2 and 3: 1 2 and 4: 1 3 and 4: 13 4 and 4: 90	Exons: 4 Promoters: 3 Introns: 29 Intergenic: 70