PROGNOS Summary for Hockemeyer_AAVS1_Ranking-TALENv2.0

The hg19 genome was searched for the TALEN pair targeting:
5'- TTTTCTGTCACCAATCCT NN..NN ACTGTGGGGTGGAGGGGA -3'

Using these RVDs:
Left:  01NG02NG03NG04HD05NG06NN07NG08HD09NI10HD11HD12NI13NI14NG15HD16HD17NG
Right: 01HD02HD03HD04HD05NG06HD07HD08NI09HD10HD11HD12HD13NI14HD15NI16NN17NG

Up to 5 mismatches were allowed in each nuclease half-site.

Allowed spacing distances were: 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26

Only heterodimers of the nuclease pair were included in the output

292 total sites were located
Mismatch Types
Genomic Regions
  0 and 0: 1
  2 and 5: 1
  3 and 5: 5
  4 and 4: 2
  4 and 5: 60
  5 and 5: 223
  Exons: 10
  Promoters: 5
  Introns: 130
  Intergenic: 147


The top 100 sites are ranked below according to parameters optimized for TALEN off-target prediction.
For the full output, click here.

This output will be deleted from the server one week after it is generated.  Please save your results.

Primer pairs were designed using the 400 bp of sequence on either side of the cleavage site to melt close to 60 °C

For easy visualization of cleavage on gels:
The smallest fragment will be longer than 130 bp, which is also the minimum separation distance between uncleaved and cleaved products.
The two cleavage products will be separated by a minimum size of 0 bp
To optimize assay conditions, the amplicons have a minimum length of 260 bp, a maximum length of 450 bp, and an optimal length of 270 bp.
Rank: 1	Homology Score: 72	TALEN Score: 100
	Genomic Region: Intron	Closest Gene: PPP1R12C	Caution! This site scores very closely to the intended nuclease target. The potential for cleavage at this site is of great concern.
Match Type: L15R
	           Left Nuclease
	         TTTTCTGTCACCAATCCT
	5'-GGGGC TTTTCTGTCACCAATCCT      GTCCCTAGTGGCCCC       ACTGTGGGGTGGAGGGGA CAGAT-3'
	3'-CCCCG AAAAGACAGTGGTTAGGA      CAGGGATCACCGGGG       TGACACCCCACCTCCCCT GTCTA-5'
	                                                       TGACACCCCACCTCCCCT
	                                                            Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 019-055627003
Forward Primer: 5'-CCAGGAAATGGGGGTGTGTC-3' Tm: 59.508
Reverse Primer: 5'-GTCCACTTCAGGACAGCATGTTTG-3' Tm: 59.038
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 131, 139
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr19:55627107-55627157

Rank: 2	Homology Score: 17	TALEN Score: 57.02
	Genomic Region: Intergenic	Closest Gene: TFEC
Match Type: R11L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-CCCCC TCCCCGCCACCCCCCAGT        AATGGGGAGAG         AGGACATTTGACAGAGAA TGAAG-3'
	3'-GGGGG AGGGGCGGTGGGGGGTCA        TTACCCCTCTC         TCCTGTAAACTGTCTCTT ACTTC-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 007-115754830
Forward Primer: 5'-CGGTTGAGACTGAAGCATGCAAAG-3' Tm: 59.038
Reverse Primer: 5'-AAGGACCACACTACTGACATTAGATCTACC-3' Tm: 60.058
Expected PCR Product Length: 274
Expected CelI Cleavage Product Sizes: 143, 131
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr7:115754948-115754994

Rank: 3	Homology Score: 10	TALEN Score: 56.2
	Genomic Region: Intron	Closest Gene: STPG1
Match Type: R11L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-ACACT TCCCCACCACACCACAAT        GCCCTGTCTCA         AGAAACTGTGACAGAATA AAATG-3'
	3'-TGTGA AGGGGTGGTGTGGTGTTA        CGGGACAGAGT         TCTTTGACACTGTCTTAT TTTAC-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 001-024697363
Forward Primer: 5'-AGCCTCCCAAAGTGCTAGGAC-3' Tm: 59.378
Reverse Primer: 5'-GTGGAGAACATCCATTCACAGGG-3' Tm: 59.148
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 135, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr1:24697473-24697519

Rank: 4	Homology Score: 10	TALEN Score: 53.2
	Genomic Region: Intron	Closest Gene: ERCC5
Match Type: L20R
	           Left Nuclease
	         TTTTCTGTCACCAATCCT
	5'-CTTTA TTTTCTTTCTGCAACCAT    GAAGTCTCTGGAAGTGGTGG    ACTGTAGGGTGGTGTGGA GTAGC-3'
	3'-GAAAT AAAAGAAAGACGTTGGTA    CTTCAGAGACCTTCACCACC    TGACATCCCACCACACCT CATCG-5'
	                                                       TGACACCCCACCTCCCCT
	                                                            Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 013-103511881
Forward Primer: 5'-TCTCTGGCTGGCAGTTGAGG-3' Tm: 59.508
Reverse Primer: 5'-CAGGTCACTGAAAACTGCAAACCG-3' Tm: 59.038
Expected PCR Product Length: 346
Expected CelI Cleavage Product Sizes: 131, 215
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr13:103511982-103512037

Rank: 5	Homology Score: 5	TALEN Score: 52.88
	Genomic Region: Intergenic	Closest Gene: MRM1
Match Type: L25R
	           Left Nuclease
	         TTTTCTGTCACCAATCCT
	5'-TTCTT TTATCTGCCCCCAACACT CCAGGCATTATGTGCATTCATAAAA  AAGGGGGGGTGGAGTGGA GAGAA-3'
	3'-AAGAA AATAGACGGGGGTTGTGA GGTCCGTAATACACGTAAGTATTTT  TTCCCCCCCACCTCACCT CTCTT-5'
	                                                       TGACACCCCACCTCCCCT
	                                                            Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 017-035084330
Forward Primer: 5'-GAGCCTCTGCTGGTTGGATG-3' Tm: 59.508
Reverse Primer: 5'-ATGTTGGGTGCTGGGGAAGG-3' Tm: 59.508
Expected PCR Product Length: 287
Expected CelI Cleavage Product Sizes: 131, 156
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr17:35084429-35084489

Rank: 6	Homology Score: 10	TALEN Score: 52.53
	Genomic Region: Intergenic	Closest Gene: MIR548X
Match Type: R21L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-AAATC TTCTCTCCACACCACAGT   GCAGCTGCTGGGGGTTGGGAG    AGGAGTGGTGTAAGCAAT TTGAG-3'
	3'-TTTAG AAGAGAGGTGTGGTGTCA   CGTCGACGACCCCCAACCCTC    TCCTCACCACATTCGTTA AACTC-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 016-009446101
Forward Primer: 5'-ATCCTTCCTCTGGGGGCACT-3' Tm: 59.508
Reverse Primer: 5'-CTCCAACCTCCCAATGAAACACC-3' Tm: 59.148
Expected PCR Product Length: 334
Expected CelI Cleavage Product Sizes: 155, 179
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr16:9446226-9446282

Rank: 7	Homology Score: 5	TALEN Score: 52.48
	Genomic Region: Intron	Closest Gene: DMD
Match Type: L23R
	           Left Nuclease
	         TTTTCTGTCACCAATCCT
	5'-TCTTC TCTTCCATCACTAATTCT  GATGAACAGCTATCAAGCTATTC   ATGGTAGGGTGGAGGGTA GGTGG-3'
	3'-AGAAG AGAAGGTAGTGATTAAGA  CTACTTGTCGATAGTTCGATAAG   TACCATCCCACCTCCCAT CCACC-5'
	                                                       TGACACCCCACCTCCCCT
	                                                            Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 00X-031479613
Forward Primer: 5'-GTCTGGACATGTCTGAATGCCTAG-3' Tm: 59.038
Reverse Primer: 5'-GGTTAGGTAAAGTGTCCCCTGC-3' Tm: 59.258
Expected PCR Product Length: 339
Expected CelI Cleavage Product Sizes: 156, 183
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chrX:31479738-31479796

Rank: 8	Homology Score: 5	TALEN Score: 52.42
	Genomic Region: Intergenic	Closest Gene: C11orf87
Match Type: R26L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-ACAAC AACACTCCACCCCACAAT TCTCTGCTGAGTGTCTTGGCTACAAG GTGGTTTGTGACAGCAAA ACTCC-3'
	3'-TGTTG TTGTGAGGTGGGGTGTTA AGAGACGACTCACAGAACCGATGTTC CACCAAACACTGTCGTTT TGAGG-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 011-109627678
Forward Primer: 5'-TCCTCCTTGGAACCCCAGATTTG-3' Tm: 59.148
Reverse Primer: 5'-TGTCTTCACTGCTGCACATTCCC-3' Tm: 59.148
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 135, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr11:109627780-109627841

Rank: 9	Homology Score: 8	TALEN Score: 52.31
	Genomic Region: Intron	Closest Gene: NINL
Match Type: R23L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-GAGAC ACACCTGCACCCCACATT  TTCCAGGCCCTGGAAGGCAGCTT   ATGGTTTGAGACAGAAAA GGAGG-3'
	3'-CTCTG TGTGGACGTGGGGTGTAA  AAGGTCCGGGACCTTCCGTCGAA   TACCAAACTCTGTCTTTT CCTCC-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 020-025439956
Forward Primer: 5'-AGTCCCTGCACTGACTCAGC-3' Tm: 59.508
Reverse Primer: 5'-GTGCTTGATGCGTGGGAGTC-3' Tm: 59.508
Expected PCR Product Length: 285
Expected CelI Cleavage Product Sizes: 134, 151
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr20:25440059-25440117

Rank: 10	Homology Score: 5	TALEN Score: 51.95
	Genomic Region: Intergenic	Closest Gene: AMIGO2
Match Type: R11L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-GGATC CTCCCACCACCCCACGAT        GTAAAACTTGA         AGGGTTGATGGCTGAAAA AATAT-3'
	3'-CCTAG GAGGGTGGTGGGGTGCTA        CATTTTGAACT         TCCCAACTACCGACTTTT TTATA-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 012-047346370
Forward Primer: 5'-CTTGGTATGTTCCAGTGTGGCC-3' Tm: 59.258
Reverse Primer: 5'-GGATGTGTGCAGGATGTACAGG-3' Tm: 59.258
Expected PCR Product Length: 272
Expected CelI Cleavage Product Sizes: 134, 138
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr12:47346479-47346525

Rank: 11	Homology Score: 5	TALEN Score: 51.8
	Genomic Region: Intron	Closest Gene: ARHGAP10
Match Type: R17L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-TCCTG TTCCCACCACCCCACAAC     CTTCTTACATCTGAACA      ATGACAGATGACAGTAAA TCCAG-3'
	3'-AGGAC AAGGGTGGTGGGGTGTTG     GAAGAATGTAGACTTGT      TACTGTCTACTGTCATTT AGGTC-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 004-148942680
Forward Primer: 5'-GTAGGTAAGCCAGGGAGCTGT-3' Tm: 59.378
Reverse Primer: 5'-CCTGCCAACACCATGTTTCCAC-3' Tm: 59.258
Expected PCR Product Length: 299
Expected CelI Cleavage Product Sizes: 158, 141
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr4:148942810-148942862

Rank: 12	Homology Score: 5	TALEN Score: 51.72
	Genomic Region: Intergenic	Closest Gene: LOC729013
Match Type: L25R
	           Left Nuclease
	         TTTTCTGTCACCAATCCT
	5'-AGGTC TCCTCTGACACCACTCCA GTGAGTAGTGGAAAGATGCCTTATT  ACTGCTGGGTGGGGGTGA AGGTC-3'
	3'-TCCAG AGGAGACTGTGGTGAGGT CACTCATCACCTTTCTACGGAATAA  TGACGACCCACCCCCACT TCCAG-5'
	                                                       TGACACCCCACCTCCCCT
	                                                            Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 011-011075984
Forward Primer: 5'-GCCTCATTACTGCTAGGTGGAAG-3' Tm: 59.148
Reverse Primer: 5'-CACCCTAACATCCAGGCATGATGA-3' Tm: 59.038
Expected PCR Product Length: 316
Expected CelI Cleavage Product Sizes: 152, 164
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr11:11076104-11076164

Rank: 13	Homology Score: 5	TALEN Score: 51.58
	Genomic Region: Intron	Closest Gene: HEG1
Match Type: R18L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-CTCTA TCCCTTCCACCTCACCAA     ATAGTCCTCGCTAGCAGA     AGGGTTGGGTACAGAACA ACAGT-3'
	3'-GAGAT AGGGAAGGTGGAGTGGTT     TATCAGGAGCGATCGTCT     TCCCAACCCATGTCTTGT TGTCA-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 003-124702567
Forward Primer: 5'-TGCAAGTCTACAACCAGGGTGTG-3' Tm: 59.148
Reverse Primer: 5'-CAGAAGGCAGCTGGAAGTCTC-3' Tm: 59.378
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 134, 136
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr3:124702672-124702725

Rank: 14	Homology Score: 8	TALEN Score: 51.36
	Genomic Region: Intergenic	Closest Gene: INADL
Match Type: R25L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-TCAAC TCCTCTCAACCCCAGAGC CATGTGTTACTCTTTGGGGAACTTC  AGGATAGCTGTCTGAAAA CAGTC-3'
	3'-AGTTG AGGAGAGTTGGGGTCTCG GTACACAATGAGAAACCCCTTGAAG  TCCTATCGACAGACTTTT GTCAG-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 001-062633493
Forward Primer: 5'-TCCTCCTGCCTAATGGCTTTACTG-3' Tm: 59.038
Reverse Primer: 5'-GCTACTGAGCCCAGCCTAATTTAC-3' Tm: 59.038
Expected PCR Product Length: 273
Expected CelI Cleavage Product Sizes: 130, 143
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr1:62633591-62633651

Rank: 15	Homology Score: 5	TALEN Score: 51.13
	Genomic Region: Intergenic	Closest Gene: C20orf197
Match Type: R16L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-GCACC TCCCATCAACCCCATAGC      AGGAATGGGGCGAACT      TTGATTGATGTAAGAAAA TGAAA-3'
	3'-CGTGG AGGGTAGTTGGGGTATCG      TCCTTACCCCGCTTGA      AACTAACTACATTCTTTT ACTTT-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 020-058618528
Forward Primer: 5'-GGGAGCTATGATCATGCCATAGG-3' Tm: 59.148
Reverse Primer: 5'-GCTCCATGTCATTGTTCTGGGC-3' Tm: 59.258
Expected PCR Product Length: 290
Expected CelI Cleavage Product Sizes: 135, 155
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr20:58618635-58618686

Rank: 16	Homology Score: 5	TALEN Score: 51.05
	Genomic Region: Intergenic	Closest Gene: CLINT1
Match Type: L15R
	           Left Nuclease
	         TTTTCTGTCACCAATCCT
	5'-TGCAC TTTTCAGCCCCCACTCCT      CCTTTATGAAGAGAA       CCTGTGTGCTGGTGGGAA GAGCT-3'
	3'-ACGTG AAAAGTCGGGGGTGAGGA      GGAAATACTTCTCTT       GGACACACGACCACCCTT CTCGA-5'
	                                                       TGACACCCCACCTCCCCT
	                                                            Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 005-157580667
Forward Primer: 5'-GTAATCCAGGGTCTCTGTTTGTCC-3' Tm: 59.038
Reverse Primer: 5'-GTCTTTGGGTGGCATTTGTGAGG-3' Tm: 59.148
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 133, 137
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr5:157580773-157580823

Rank: 17	Homology Score: 10	TALEN Score: 50.69
	Genomic Region: Intron	Closest Gene: MYT1L
Match Type: R15L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-GAATG TTCCCTCCAACCCAGAGT      GCTGAGAAGGCTGGC       AGGATAGAGGCCAGAACA TGTCT-3'
	3'-CTTAC AAGGGAGGTTGGGTCTCA      CGACTCTTCCGACCG       TCCTATCTCCGGTCTTGT ACAGA-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 002-001827851
Forward Primer: 5'-GGGGGTTGGGGAACGTTTTTAG-3' Tm: 59.258
Reverse Primer: 5'-TCAGGACGAGAGCAGCTCAAATG-3' Tm: 59.148
Expected PCR Product Length: 446
Expected CelI Cleavage Product Sizes: 315, 131
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr2:1828139-1828189

Rank: 18	Homology Score: 5	TALEN Score: 50.62
	Genomic Region: Intron	Closest Gene: TBC1D12
Match Type: L19R
	           Left Nuclease
	         TTTTCTGTCACCAATCCT
	5'-CTTTC TTTTCTGTAGCAAATTCT    AGATCTATTTTTTCTTATT     TCTTTGAGGTGGGGAGGA GTAGT-3'
	3'-GAAAG AAAAGACATCGTTTAAGA    TCTAGATAAAAAAGAATAA     AGAAACTCCACCCCTCCT CATCA-5'
	                                                       TGACACCCCACCTCCCCT
	                                                            Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 010-096170810
Forward Primer: 5'-GGCATGCCAGAGATAGGACTATTC-3' Tm: 59.038
Reverse Primer: 5'-GTGCTACAGTGGCAACCTTCATG-3' Tm: 59.148
Expected PCR Product Length: 330
Expected CelI Cleavage Product Sizes: 191, 139
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr10:96170972-96171026

Rank: 19	Homology Score: 5	TALEN Score: 50.5
	Genomic Region: Intron	Closest Gene: NFATC2
Match Type: L25R
	           Left Nuclease
	         TTTTCTGTCACCAATCCT
	5'-TCGGG TTTGCTGTCACAACAACT GCAGGATGACCCGGTGCTGGGCAGC  ACTGCTGGCTGGAGGTGA GAAAT-3'
	3'-AGCCC AAACGACAGTGTTGTTGA CGTCCTACTGGGCCACGACCCGTCG  TGACGACCGACCTCCACT CTTTA-5'
	                                                       TGACACCCCACCTCCCCT
	                                                            Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 020-050029460
Forward Primer: 5'-AGAAGCACAGGATGAGGCCG-3' Tm: 59.508
Reverse Primer: 5'-CAGTGGATTGACAGAGGCAGATG-3' Tm: 59.148
Expected PCR Product Length: 336
Expected CelI Cleavage Product Sizes: 205, 131
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr20:50029633-50029693

Rank: 20	Homology Score: 5	TALEN Score: 50.34
	Genomic Region: Intron	Closest Gene: TEX264
Match Type: L24R
	           Left Nuclease
	         TTTTCTGTCACCAATCCT
	5'-TTCCC TTTTTTGCCAACACTCCT  GAGTGAGTAAGCAGGTGTTGGTGC  AGGGTGGGGTGGTGGTGG GGAAG-3'
	3'-AAGGG AAAAAACGGTTGTGAGGA  CTCACTCATTCGTCCACAACCACG  TCCCACCCCACCACCACC CCTTC-5'
	                                                       TGACACCCCACCTCCCCT
	                                                            Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 003-051729704
Forward Primer: 5'-GGAGATGAACTCAGGCTGAAGTG-3' Tm: 59.148
Reverse Primer: 5'-TTCCCTCCCACCTGGGTCTT-3' Tm: 59.508
Expected PCR Product Length: 271
Expected CelI Cleavage Product Sizes: 141, 130
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr3:51729813-51729872

Rank: 21	Homology Score: 5	TALEN Score: 50.25
	Genomic Region: Intergenic	Closest Gene: SMAD3
Match Type: R13L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-TGTTC TTCCACCCAGCCCTCAGT       GCTCTGCTGAGGC        AGGATGGGTGGCTGGAAA AATGG-3'
	3'-ACAAG AAGGTGGGTCGGGAGTCA       CGAGACGACTCCG        TCCTACCCACCGACCTTT TTACC-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 015-067305574
Forward Primer: 5'-CCCCACCTTGCTGAATGGAC-3' Tm: 59.508
Reverse Primer: 5'-CCAGTCCTTGATCTCTTCCTGAGT-3' Tm: 59.038
Expected PCR Product Length: 275
Expected CelI Cleavage Product Sizes: 142, 133
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr15:67305690-67305738

Rank: 22	Homology Score: 5	TALEN Score: 50.22
	Genomic Region: Intergenic	Closest Gene: CD6
Match Type: R22L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-TGGCT TCCCCTCCACTCCTGGGA   GACCTCATTATAATACGCCTCA   AGGATCTGGGACAGGAAA CACAA-3'
	3'-ACCGA AGGGGAGGTGAGGACCCT   CTGGAGTAATATTATGCGGAGT   TCCTAGACCCTGTCCTTT GTGTT-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 011-060790178
Forward Primer: 5'-GCCTGTGCTGAGACTGGGAA-3' Tm: 59.508
Reverse Primer: 5'-AGCTGTCAGTCAAATTGCTGGGTG-3' Tm: 59.038
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 131, 139
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr11:60790278-60790335

Rank: 23	Homology Score: 10	TALEN Score: 50.06
	Genomic Region: Intergenic	Closest Gene: FOXP1
Match Type: R20L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-TGAAA TGCCCCCCACCCCAGAGT    ATATTCATTAGGACTCTTTG    ATTATAAGAGACAGAAAA TCAAC-3'
	3'-ACTTT ACGGGGGGTGGGGTCTCA    TATAAGTAATCCTGAGAAAC    TAATATTCTCTGTCTTTT AGTTG-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 003-070995805
Forward Primer: 5'-GCCGTTGCTTTTGCCTTGAACATC-3' Tm: 59.038
Reverse Primer: 5'-TACTTGAGTCCCTGGATCTGGC-3' Tm: 59.258
Expected PCR Product Length: 306
Expected CelI Cleavage Product Sizes: 176, 130
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr3:70995951-70996006

Rank: 24	Homology Score: 5	TALEN Score: 50
	Genomic Region: Intergenic	Closest Gene: CDKL5
Match Type: R13L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-CCCTC TCCCCTCTACCTCCCTAT       GGTCAGAGAAGTT        AGGCTTGGTGAGAGAACT GACCA-3'
	3'-GGGAG AGGGGAGATGGAGGGATA       CCAGTCTCTTCAA        TCCGAACCACTCTCTTGA CTGGT-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 00X-018438373
Forward Primer: 5'-CAGGTCATGACCATCAGTGATCAG-3' Tm: 59.038
Reverse Primer: 5'-CACCTGTCAGGCTTGTGGCT-3' Tm: 59.508
Expected PCR Product Length: 278
Expected CelI Cleavage Product Sizes: 147, 131
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chrX:18438494-18438542

Rank: 25	Homology Score: 5	TALEN Score: 49.96
	Genomic Region: Intergenic	Closest Gene: TMTC1
Match Type: L12R
	           Left Nuclease
	         TTTTCTGTCACCAATCCT
	5'-GATTA TTTTCTAACAACCTTCCT        ACTGAGGAACAT        ACTCTGGGATGGATTGGA AGTGC-3'
	3'-CTAAT AAAAGATTGTTGGAAGGA        TGACTCCTTGTA        TGAGACCCTACCTAACCT TCACG-5'
	                                                       TGACACCCCACCTCCCCT
	                                                            Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 012-030180063
Forward Primer: 5'-CTATAAGGCTGCTGGGCCTTTC-3' Tm: 59.258
Reverse Primer: 5'-GAGGCAAACCTACTTTATGGAGGG-3' Tm: 59.038
Expected PCR Product Length: 333
Expected CelI Cleavage Product Sizes: 131, 202
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr12:30180168-30180215

Rank: 26	Homology Score: 5	TALEN Score: 49.96
	Genomic Region: Intergenic	Closest Gene: ZNF827
Match Type: R20L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-AGATT TCCTCTCCACACCACATG    GCCACTGCTGGGGCTGGGGG    AGGAGAGGTGTCAGCAAT TCAAG-3'
	3'-TCTAA AGGAGAGGTGTGGTGTAC    CGGTGACGACCCCGACCCCC    TCCTCTCCACAGTCGTTA AGTTC-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 004-146944903
Forward Primer: 5'-CAGAGGAGTTCAGCTCAGTTTTGC-3' Tm: 59.038
Reverse Primer: 5'-CCCTCCCCCAAGGACAACAA-3' Tm: 59.508
Expected PCR Product Length: 307
Expected CelI Cleavage Product Sizes: 135, 172
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr4:146945008-146945063

Rank: 27	Homology Score: 5	TALEN Score: 49.95
	Genomic Region: Intron	Closest Gene: LOC730102
Match Type: R25L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-AACCA TCCCCTCCAACCCCCATC AGTCCATGGAAAAACTGTCTTCCAC  AGAACTGGTGCCAAAAAG GTTGC-3'
	3'-TTGGT AGGGGAGGTTGGGGGTAG TCAGGTACCTTTTTGACAGAAGGTG  TCTTGACCACGGTTTTTC CAACG-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 001-178005473
Forward Primer: 5'-CTCACAGAAGTGCGAACCGTATTG-3' Tm: 59.038
Reverse Primer: 5'-CACCAATATGGCCACCTCTGTAC-3' Tm: 59.148
Expected PCR Product Length: 315
Expected CelI Cleavage Product Sizes: 148, 167
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr1:178005589-178005649

Rank: 28	Homology Score: 5	TALEN Score: 49.84
	Genomic Region: Intergenic	Closest Gene: FAXC
Match Type: L26R
	           Left Nuclease
	         TTTTCTGTCACCAATCCT
	5'-TGTCC ATTTCTGTAACAAATCTT TATTGAGCATTTATTACATGTACCAA GCTGTGAGGGGAAGTGGA TTGTG-3'
	3'-ACAGG TAAAGACATTGTTTAGAA ATAACTCGTAAATAATGTACATGGTT CGACACTCCCCTTCACCT AACAC-5'
	                                                       TGACACCCCACCTCCCCT
	                                                            Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 006-099703892
Forward Primer: 5'-GGACTGCATAAACCACTTGAGCC-3' Tm: 59.148
Reverse Primer: 5'-GCAGGACTCTCTAGCTGGCTAT-3' Tm: 59.258
Expected PCR Product Length: 330
Expected CelI Cleavage Product Sizes: 200, 130
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr6:99704059-99704120

Rank: 29	Homology Score: 5	TALEN Score: 49.8
	Genomic Region: Exon	Closest Gene: CACNG3
Match Type: R26L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-ACTCT CCCCCTCCAACCCCCAGC CGTCCAGAGTACCATGAAGAATTATG AGGATGTGTGACAGAGGT ATCCA-3'
	3'-TGAGA GGGGGAGGTTGGGGGTCG GCAGGTCTCATGGTACTTCTTAATAC TCCTACACACTGTCTCCA TAGGT-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 016-024267933
Forward Primer: 5'-CAAAGTCCCTGTGGATGCTGACAA-3' Tm: 59.038
Reverse Primer: 5'-TAGTCCTGCACACACCTCTGG-3' Tm: 59.378
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 135, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr16:24268035-24268096

Rank: 30	Homology Score: 5	TALEN Score: 49.8
	Genomic Region: Intergenic	Closest Gene: ABRA
Match Type: R12L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-CTGTT TCCCCACCCTCCCACATT        ATGTACCCTGGG        AGGAAGGGTGCCAGAGAG AGGCT-3'
	3'-GACAA AGGGGTGGGAGGGTGTAA        TACATGGGACCC        TCCTTCCCACGGTCTCTC TCCGA-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 008-107822970
Forward Primer: 5'-GGCTAAGTGTGAGAATCGAGTGAG-3' Tm: 59.038
Reverse Primer: 5'-GTTAGTGAGACAGCATGTCCCTTC-3' Tm: 59.038
Expected PCR Product Length: 280
Expected CelI Cleavage Product Sizes: 150, 130
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr8:107823094-107823141

Rank: 31	Homology Score: 5	TALEN Score: 49.78
	Genomic Region: Intergenic	Closest Gene: RPRM
Match Type: L14R
	           Left Nuclease
	         TTTTCTGTCACCAATCCT
	5'-ACTGT TTTTCTGACAGAAACCCT       TTGCAGAGTTTTTG       GCTGGGGGGTGGGGAGGT GGTTG-3'
	3'-TGACA AAAAGACTGTCTTTGGGA       AACGTCTCAAAAAC       CGACCCCCCACCCCTCCA CCAAC-5'
	                                                       TGACACCCCACCTCCCCT
	                                                            Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 002-154289086
Forward Primer: 5'-GGTTTCAAGTTTCACCTATTGCTTGGCAG-3' Tm: 60.178
Reverse Primer: 5'-CGTGTTATGTGTGTTTAGCCCCC-3' Tm: 59.148
Expected PCR Product Length: 411
Expected CelI Cleavage Product Sizes: 134, 277
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr2:154289193-154289242

Rank: 32	Homology Score: 2	TALEN Score: 49.75
	Genomic Region: Intergenic	Closest Gene: POM121L12
Match Type: L23R
	           Left Nuclease
	         TTTTCTGTCACCAATCCT
	5'-CTGAA TTTGCAGCCCCAAATCCT  GCCCACTGAGCAGACTTCTTTCT   ACCTTGGGGTGGGGTTGA AAGCG-3'
	3'-GACTT AAACGTCGGGGTTTAGGA  CGGGTGACTCGTCTGAAGAAAGA   TGGAACCCCACCCCAACT TTCGC-5'
	                                                       TGACACCCCACCTCCCCT
	                                                            Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 007-052309823
Forward Primer: 5'-CCTGAGTCCCTGCTGCATTAATTC-3' Tm: 59.038
Reverse Primer: 5'-ACTCTGTGTCCTCAACGGTGTTC-3' Tm: 59.148
Expected PCR Product Length: 341
Expected CelI Cleavage Product Sizes: 210, 131
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr7:52310002-52310060

Rank: 33	Homology Score: 5	TALEN Score: 49.69
	Genomic Region: Intron	Closest Gene: BOP1
Match Type: L22R
	           Left Nuclease
	         TTTTCTGTCACCAATCCT
	5'-CCAAA TTTCCTCTCCCTAATCCT   GCAGGAGCCCACAGCTGGCCAG   AGGGAGGGGAGGAGAGGA CAAGC-3'
	3'-GGTTT AAAGGAGAGGGATTAGGA   CGTCCTCGGGTGTCGACCGGTC   TCCCTCCCCTCCTCTCCT GTTCG-5'
	                                                       TGACACCCCACCTCCCCT
	                                                            Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 008-145493530
Forward Primer: 5'-GAGCCATTAAGGGCTAGAGGG-3' Tm: 59.378
Reverse Primer: 5'-GGAAAGCTCCCAAGAAGGGAAG-3' Tm: 59.258
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 135, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr8:145493634-145493691

Rank: 34	Homology Score: 5	TALEN Score: 49.69
	Genomic Region: Intergenic	Closest Gene: SCXB
Match Type: L22R
	           Left Nuclease
	         TTTTCTGTCACCAATCCT
	5'-CCAAA TTTCCTCTCCCTAATCCT   GCAGGAGCCCACAGCTGGCCAG   AGGGAGGGGAGGAGAGGA CAAGC-3'
	3'-GGTTT AAAGGAGAGGGATTAGGA   CGTCCTCGGGTGTCGACCGGTC   TCCCTCCCCTCCTCTCCT GTTCG-5'
	                                                       TGACACCCCACCTCCCCT
	                                                            Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 008-145324445
Forward Primer: 5'-GAGCCATTAAGGGCTAGAGGG-3' Tm: 59.378
Reverse Primer: 5'-GGAAAGCTCCCAAGAAGGGAAG-3' Tm: 59.258
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 135, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr8:145324549-145324606

Rank: 35	Homology Score: 5	TALEN Score: 49.62
	Genomic Region: Intergenic	Closest Gene: FBN2
Match Type: R20L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-GACTT CCACCTCCACCTAACAGT    AATGAGATGCCCCTGTCTGC    TGGGATGGTGTCAGAAGA TACCT-3'
	3'-CTGAA GGTGGAGGTGGATTGTCA    TTACTCTACGGGGACAGACG    ACCCTACCACAGTCTTCT ATGGA-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 005-127879056
Forward Primer: 5'-CTCCTGGATAGTGTCAGAGAAAGC-3' Tm: 59.038
Reverse Primer: 5'-CTGCTCCCAATGTAGTTTCCCATG-3' Tm: 59.038
Expected PCR Product Length: 267
Expected CelI Cleavage Product Sizes: 136, 131
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr5:127879162-127879217

Rank: 36	Homology Score: 2	TALEN Score: 49.51
	Genomic Region: Intergenic	Closest Gene: ADTRP
Match Type: R14L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-TAGCT TCCCTCCCACCCCCCACC       CTGGAACAACAGAT       TGGGTTAGTGCTAGAAAA TGTCT-3'
	3'-ATCGA AGGGAGGGTGGGGGGTGG       GACCTTGTTGTCTA       ACCCAATCACGATCTTTT ACAGA-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Warning! Primer pair was generated using suboptimal parameters. 
Primer Index: 006-011853744
Forward Primer: 5'-GTCTACGGATCTAACACTCCAAAAATCTTAGC-3' Tm: 59.848
Reverse Primer: 5'-CTAGGGGGCTGAAATGGAACAAC-3' Tm: 59.148
Expected PCR Product Length: 325
Expected CelI Cleavage Product Sizes: 172, 153
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr6:11853889-11853938

Rank: 37	Homology Score: 5	TALEN Score: 49.48
	Genomic Region: Intergenic	Closest Gene: TSPAN9
Match Type: L12R
	           Left Nuclease
	         TTTTCTGTCACCAATCCT
	5'-TTGGA TTTTCTGACACAAACCCA        TCAGGATCTGGA        ACAGTGGCGTCCAGGAGA ACTTT-3'
	3'-AACCT AAAAGACTGTGTTTGGGT        AGTCCTAGACCT        TGTCACCGCAGGTCCTCT TGAAA-5'
	                                                       TGACACCCCACCTCCCCT
	                                                            Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 012-003414995
Forward Primer: 5'-TGACGTTCCCTGGGCCAAGA-3' Tm: 59.508
Reverse Primer: 5'-AAAATCCAGTTCCTTGCTCGCACC-3' Tm: 59.038
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 132, 138
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr12:3415101-3415148

Rank: 38	Homology Score: 5	TALEN Score: 49.46
	Genomic Region: Intergenic	Closest Gene: SYT6
Match Type: L18R
	           Left Nuclease
	         TTTTCTGTCACCAATCCT
	5'-TTTCC CTTTCTGATAATAATCCT     ATAATGAACTCTAGGCAC     TCTGCTGGGTGGAGAGGA TATAA-3'
	3'-AAAGG GAAAGACTATTATTAGGA     TATTACTTGAGATCCGTG     AGACGACCCACCTCTCCT ATATT-5'
	                                                       TGACACCCCACCTCCCCT
	                                                            Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 001-114585589
Forward Primer: 5'-CCCCATAAATGATCTGCATCCAGG-3' Tm: 59.038
Reverse Primer: 5'-CGCTGAGAAGGCCAGATGAG-3' Tm: 59.508
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 135, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr1:114585695-114585748

Rank: 39	Homology Score: 5	TALEN Score: 49.38
	Genomic Region: Intron	Closest Gene: PTPRN2
Match Type: L25R
	           Left Nuclease
	         TTTTCTGTCACCAATCCT
	5'-AGAGA TCTGCTGTGACCACACCT GCAGGGTACTCAGGCCACCTTTCAT  ACTGGGGTGAGGAGAGGA AGTAA-3'
	3'-TCTCT AGACGACACTGGTGTGGA CGTCCCATGAGTCCGGTGGAAAGTA  TGACCCCACTCCTCTCCT TCATT-5'
	                                                       TGACACCCCACCTCCCCT
	                                                            Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 007-158192599
Forward Primer: 5'-TAGTCCCCAGGCACTTGCAG-3' Tm: 59.508
Reverse Primer: 5'-GCCTCGTAAGTTTAGGCATCCTC-3' Tm: 59.148
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 137, 133
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr7:158192704-158192764

Rank: 40	Homology Score: 2	TALEN Score: 49.38
	Genomic Region: Intron	Closest Gene: SMOC2
Match Type: R25L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-CCTCC CCCCCTCCCCCACAAAGA TTTGAAGCCTAACATTGCTGGGGGC  AGGATTAGAGGTTGAAAA CAAAG-3'
	3'-GGAGG GGGGGAGGGGGTGTTTCT AAACTTCGGATTGTAACGACCCCCG  TCCTAATCTCCAACTTTT GTTTC-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 006-168932997
Forward Primer: 5'-TCCTTCGTCCTGGGATTGGG-3' Tm: 59.508
Reverse Primer: 5'-GGGTGCACAGTTACAGAGGAG-3' Tm: 59.378
Expected PCR Product Length: 280
Expected CelI Cleavage Product Sizes: 149, 131
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr6:168933114-168933174

Rank: 41	Homology Score: 5	TALEN Score: 49.37
	Genomic Region: Exon	Closest Gene: CATSPERG
Match Type: R24L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-TCAAT CCCCTCCAACCCCATAGT  TTTCTACCCCTTCTTCTTGATTCA  AGATTTGGTGACAGGAGA CTCCG-3'
	3'-AGTTA GGGGAGGTTGGGGTATCA  AAAGATGGGGAAGAAGAACTAAGT  TCTAAACCACTGTCCTCT GAGGC-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 019-038858026
Forward Primer: 5'-TTCCTCTCCCCGCAGACTAC-3' Tm: 59.508
Reverse Primer: 5'-AGTGCACACAGACTGGCCTC-3' Tm: 59.508
Expected PCR Product Length: 281
Expected CelI Cleavage Product Sizes: 138, 143
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr19:38858132-38858191

Rank: 42	Homology Score: 5	TALEN Score: 49.32
	Genomic Region: Intron	Closest Gene: DACH2
Match Type: R16L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-TATGA TCCCTGCCATCCAACAGT      TAGTGTATTTATATTC      ATGTTTAGTGACATAAAT AGCAA-3'
	3'-ATACT AGGGACGGTAGGTTGTCA      ATCACATAAATATAAG      TACAAATCACTGTATTTA TCGTT-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 00X-085476489
Forward Primer: 5'-GGAAATGTGAGGCCATCAGCTTTG-3' Tm: 59.038
Reverse Primer: 5'-CAGCCCCCAGAACTGTGAGA-3' Tm: 59.508
Expected PCR Product Length: 346
Expected CelI Cleavage Product Sizes: 194, 152
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chrX:85476655-85476706

Rank: 43	Homology Score: 2	TALEN Score: 49.3
	Genomic Region: Intron	Closest Gene: KIRREL3
Match Type: R13L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-GTGTT TTCACTCACCACCACAGT       CAGGGCTGGCACA        GGGACTGGGGAATGAAAA ATAAA-3'
	3'-CACAA AAGTGAGTGGTGGTGTCA       GTCCCGACCGTGT        CCCTGACCCCTTACTTTT TATTT-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 011-126543310
Forward Primer: 5'-CCAGCAGCTCTGGAAATTGGATC-3' Tm: 59.148
Reverse Primer: 5'-GAGGAAGATCTTACAGACCCAACC-3' Tm: 59.038
Expected PCR Product Length: 345
Expected CelI Cleavage Product Sizes: 187, 158
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr11:126543471-126543519

Rank: 44	Homology Score: 2	TALEN Score: 49.28
	Genomic Region: Intron	Closest Gene: ABCB7
Match Type: L16R
	           Left Nuclease
	         TTTTCTGTCACCAATCCT
	5'-CCTAA ATTGCTGACACCACCCCT      CCCCGACACCGCAGAA      GCAGTGGTGTGGTGTGGA GAGCA-3'
	3'-GGATT TAACGACTGTGGTGGGGA      GGGGCTGTGGCGTCTT      CGTCACCACACCACACCT CTCGT-5'
	                                                       TGACACCCCACCTCCCCT
	                                                            Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 00X-074326678
Forward Primer: 5'-CCCTTCCCTTCAAGGACACC-3' Tm: 59.508
Reverse Primer: 5'-GCTGAGTTTCGTCCTGTTTCTGG-3' Tm: 59.148
Expected PCR Product Length: 306
Expected CelI Cleavage Product Sizes: 173, 133
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chrX:74326823-74326874

Rank: 45	Homology Score: 2	TALEN Score: 49.26
	Genomic Region: Intergenic	Closest Gene: SALL4
Match Type: L14R
	           Left Nuclease
	         TTTTCTGTCACCAATCCT
	5'-AAAAA ATCTCTGTCGCCAAACCA       TCTGAACTCTAGTG       AAAGTGGGGTGGGTGTGA ACAAA-3'
	3'-TTTTT TAGAGACAGCGGTTTGGT       AGACTTGAGATCAC       TTTCACCCCACCCACACT TGTTT-5'
	                                                       TGACACCCCACCTCCCCT
	                                                            Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 020-050472318
Forward Primer: 5'-CTGGGTCATGTGGTACTGCATGT-3' Tm: 59.148
Reverse Primer: 5'-CTGCAGTTGATCCATCCAGACC-3' Tm: 59.258
Expected PCR Product Length: 285
Expected CelI Cleavage Product Sizes: 155, 130
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr20:50472446-50472495

Rank: 46	Homology Score: 2	TALEN Score: 49.24
	Genomic Region: Intergenic	Closest Gene: MIR4643
Match Type: R11L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-GACCC TCCGCACCACCCCTCAAC        ACCTCTCCCAT         ATGAGGGGTGACAGCTAA TTGTT-3'
	3'-CTGGG AGGCGTGGTGGGGAGTTG        TGGAGAGGGTA         TACTCCCCACTGTCGATT AACAA-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 006-092651079
Forward Primer: 5'-CAGCAAGGAGGCCAAGAACTCA-3' Tm: 59.258
Reverse Primer: 5'-GTGTGTGGGCCTCAAGTTTTGTAC-3' Tm: 59.038
Expected PCR Product Length: 352
Expected CelI Cleavage Product Sizes: 151, 201
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr6:92651205-92651251

Rank: 47	Homology Score: 5	TALEN Score: 49.23
	Genomic Region: Intron	Closest Gene: PVRL1
Match Type: R17L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-TAAAG TCCTCTGCCACCCACACT     CTTCATGAGATCCCAAA      AGGATTGATCATGGAAAA TGCAA-3'
	3'-ATTTC AGGAGACGGTGGGTGTGA     GAAGTACTCTAGGGTTT      TCCTAACTAGTACCTTTT ACGTT-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 011-119574363
Forward Primer: 5'-CAACGAGGCTGACGCTCTTTC-3' Tm: 59.378
Reverse Primer: 5'-CTGAGCACCTGTGTGCCAGT-3' Tm: 59.508
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 135, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr11:119574470-119574522

Rank: 48	Homology Score: 2	TALEN Score: 49.22
	Genomic Region: Intron	Closest Gene: LYPD6B
Match Type: R23L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-CCTTA TCTCCTCCTTCCTAAAGT  AAGGTCTTTAGATATTCTTTTGA   AGTTTTGTTGATAGTAAA CTCCT-3'
	3'-GGAAT AGAGGAGGAAGGATTTCA  TTCCAGAAATCTATAAGAAAACT   TCAAAACAACTATCATTT GAGGA-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 002-149982161
Forward Primer: 5'-GGCATCTTCGAGTACCTTAACTCC-3' Tm: 59.038
Reverse Primer: 5'-CTCTTCTCAAGTTCAAGGGTCAGTTGTAC-3' Tm: 60.178
Expected PCR Product Length: 409
Expected CelI Cleavage Product Sizes: 219, 190
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr2:149982349-149982407

Rank: 49	Homology Score: 2	TALEN Score: 49.2
	Genomic Region: Intergenic	Closest Gene: RTN4RL2
Match Type: R13L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-TCGTC CCCCCTCCCCCCAACCCT       AACCGGAGCTAGG        TGGGGTGGGGACAGCAAA TGTGG-3'
	3'-AGCAG GGGGGAGGGGGGTTGGGA       TTGGCCTCGATCC        ACCCCACCCCTGTCGTTT ACACC-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 011-057225300
Forward Primer: 5'-AGCCTCACCGTACAGATGAGGA-3' Tm: 59.258
Reverse Primer: 5'-TTTAGGTGGCTGGCTGGTGG-3' Tm: 59.508
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 130, 140
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr11:57225404-57225452

Rank: 50	Homology Score: 5	TALEN Score: 49.17
	Genomic Region: Intergenic	Closest Gene: NEK7
Match Type: L25R
	           Left Nuclease
	         TTTTCTGTCACCAATCCT
	5'-GCTGA TTTCCTGACACCAAGGCT CCTCAGCTGAGGCTCCCACCTGCAA  GCTGTGAGCTGGAGATGA ATATC-3'
	3'-CGACT AAAGGACTGTGGTTCCGA GGAGTCGACTCCGAGGGTGGACGTT  CGACACTCGACCTCTACT TATAG-5'
	                                                       TGACACCCCACCTCCCCT
	                                                            Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 001-198360906
Forward Primer: 5'-GGTTTTATAGCCCTTCTCCTGCC-3' Tm: 59.148
Reverse Primer: 5'-GTTGTGGGATTTGAGCTGGTGG-3' Tm: 59.258
Expected PCR Product Length: 300
Expected CelI Cleavage Product Sizes: 130, 170
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr1:198361004-198361064

Rank: 51	Homology Score: 5	TALEN Score: 49.09
	Genomic Region: Intron	Closest Gene: LRRC4C
Match Type: L14R
	           Left Nuclease
	         TTTTCTGTCACCAATCCT
	5'-TAAAA TTGTCTGTCACACATTGT       TCTGTTAATTGCCA       ACTGGGGGGGGTAGGGGG AGGAA-3'
	3'-ATTTT AACAGACAGTGTGTAACA       AGACAATTAACGGT       TGACCCCCCCCATCCCCC TCCTT-5'
	                                                       TGACACCCCACCTCCCCT
	                                                            Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 011-040808649
Forward Primer: 5'-CCCATGTCTCAGTCTCTGTTCTAC-3' Tm: 59.038
Reverse Primer: 5'-CTCACTTGTCAGCAAGCACAAAGG-3' Tm: 59.038
Expected PCR Product Length: 309
Expected CelI Cleavage Product Sizes: 155, 154
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr11:40808777-40808826

Rank: 52	Homology Score: 2	TALEN Score: 49.06
	Genomic Region: Intergenic	Closest Gene: LMO1
Match Type: L22R
	           Left Nuclease
	         TTTTCTGTCACCAATCCT
	5'-ACCTT TATACTGTCCCCAAGCCC   CCACTTATCAAAACACATTCTG   ATTGAGGGGTAGAGAGAA GGGAG-3'
	3'-TGGAA ATATGACAGGGGTTCGGG   GGTGAATAGTTTTGTGTAAGAC   TAACTCCCCATCTCTCTT CCCTC-5'
	                                                       TGACACCCCACCTCCCCT
	                                                            Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 011-008291463
Forward Primer: 5'-GTTTCCTCGGGCAATGAATGAGC-3' Tm: 59.148
Reverse Primer: 5'-GCACAGTCCTCCTTTCTTCACC-3' Tm: 59.258
Expected PCR Product Length: 311
Expected CelI Cleavage Product Sizes: 155, 156
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr11:8291587-8291644

Rank: 53	Homology Score: 5	TALEN Score: 49.01
	Genomic Region: Exon	Closest Gene: CDKAL1
Match Type: R16L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-TTCCC TCCCCTTCTCTCCACAGA      TTGGTGAAAGACAACA      AGTGTTAGTAACAGAAGA ATCTT-3'
	3'-AAGGG AGGGGAAGAGAGGTGTCT      AACCACTTTCTGTTGT      TCACAATCATTGTCTTCT TAGAA-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 006-021198081
Forward Primer: 5'-CCGTGATTTGAACAGGGTTCGC-3' Tm: 59.258
Reverse Primer: 5'-CTCTCCCCTTTAAATGCCCTTTGC-3' Tm: 59.038
Expected PCR Product Length: 341
Expected CelI Cleavage Product Sizes: 182, 159
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr6:21198235-21198286

Rank: 54	Homology Score: 2	TALEN Score: 48.97
	Genomic Region: Intergenic	Closest Gene: LGALSL
Match Type: R26L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-CTGCC TCCCCTCCCCACAATATT TACAAGCATTGATTTTCTAGAAGGGA AGGGGTGGGGAGGGAAAA AGGAG-3'
	3'-GACGG AGGGGAGGGGTGTTATAA ATGTTCGTAACTAAAAGATCTTCCCT TCCCCACCCCTCCCTTTT TCCTC-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 002-064716657
Forward Primer: 5'-GAAATGCATCCCCTCTAGCCTG-3' Tm: 59.258
Reverse Primer: 5'-GCTGCATGGACAGTGCTCACA-3' Tm: 59.378
Expected PCR Product Length: 315
Expected CelI Cleavage Product Sizes: 131, 184
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr2:64716755-64716816

Rank: 55	Homology Score: 2	TALEN Score: 48.94
	Genomic Region: Intron	Closest Gene: CDKAL1
Match Type: L17R
	           Left Nuclease
	         TTTTCTGTCACCAATCCT
	5'-TCCCC TTTTATGTCAACAATTAA     AAAATGTTATATACAGT      ATTGTTGGGTGTAGGTAA GAAAA-3'
	3'-AGGGG AAAATACAGTTGTTAATT     TTTTACAATATATGTCA      TAACAACCCACATCCATT CTTTT-5'
	                                                       TGACACCCCACCTCCCCT
	                                                            Right Nuclease
Warning! Primer pair was generated using suboptimal parameters. 
Primer Index: 006-021174562
Forward Primer: 5'-GTCGGTATAATCTTAGTCAAAAGTTCAACAGGG-3' Tm: 59.758
Reverse Primer: 5'-TAGTTAAATTGCCCTCCAAAATGGCCTTATCAATG-3' Tm: 59.578
Expected PCR Product Length: 406
Expected CelI Cleavage Product Sizes: 275, 131
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr6:21174809-21174861

Rank: 56	Homology Score: 5	TALEN Score: 48.89
	Genomic Region: Intergenic	Closest Gene: ZBTB43
Match Type: R12L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-ATCCT ACCCCTCCGCCCCTCTGT        GTGGGGAGGCAG        AGGACAGGTGACAGCTCA GACAC-3'
	3'-TAGGA TGGGGAGGCGGGGAGACA        CACCCCTCCGTC        TCCTGTCCACTGTCGAGT CTGTG-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 009-129555142
Forward Primer: 5'-TTGACCCCTTCATGCCCCCT-3' Tm: 59.508
Reverse Primer: 5'-CCTCTTCCTGGAGCAGGGAT-3' Tm: 59.508
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 139, 131
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr9:129555255-129555302

Rank: 57	Homology Score: 5	TALEN Score: 48.72
	Genomic Region: Intron	Closest Gene: KIAA1217
Match Type: L20R
	           Left Nuclease
	         TTTTCTGTCACCAATCCT
	5'-GACAT TTTTCTTTTATCCATCCT    ACAGCACTGCACAATTATTG    ATTGTGGCTTGGAAGCGA AATGA-3'
	3'-CTGTA AAAAGAAAATAGGTAGGA    TGTCGTGACGTGTTAATAAC    TAACACCGAACCTTCGCT TTACT-5'
	                                                       TGACACCCCACCTCCCCT
	                                                            Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 010-024751913
Forward Primer: 5'-GGTGGATGTGCAGAGATGGAG-3' Tm: 59.378
Reverse Primer: 5'-CTCTACTGAGCCCTCCAGTG-3' Tm: 59.508
Expected PCR Product Length: 343
Expected CelI Cleavage Product Sizes: 208, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr10:24752091-24752146

Rank: 58	Homology Score: 5	TALEN Score: 48.71
	Genomic Region: Intron	Closest Gene: KLF7
Match Type: L20R
	           Left Nuclease
	         TTTTCTGTCACCAATCCT
	5'-TCATC ATGTCTGTCACCAAACAT    GACAAATATTTACCCTTCCA    ACTGTATGGTGGACTGGT TCTAA-3'
	3'-AGTAG TACAGACAGTGGTTTGTA    CTGTTTATAAATGGGAAGGT    TGACATACCACCTGACCA AGATT-5'
	                                                       TGACACCCCACCTCCCCT
	                                                            Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 002-207954053
Forward Primer: 5'-GTTTTAGCCACAGGGAAGCTGC-3' Tm: 59.258
Reverse Primer: 5'-GAAGCTGATCCCAAGAAGTTTGGC-3' Tm: 59.038
Expected PCR Product Length: 327
Expected CelI Cleavage Product Sizes: 171, 156
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr2:207954194-207954249

Rank: 59	Homology Score: 2	TALEN Score: 48.71
	Genomic Region: Intron	Closest Gene: NCMAP
Match Type: R17L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-CCTTG TCCCCTCCCCACCCAAGC     ATCTGGCGGAGGGGCCG      AGGCTCGCTGGAAGAAAA GGGAG-3'
	3'-GGAAC AGGGGAGGGGTGGGTTCG     TAGACCGCCTCCCCGGC      TCCGAGCGACCTTCTTTT CCCTC-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 001-024882812
Forward Primer: 5'-CATCTGTTGGAGGAAGTGCCTG-3' Tm: 59.258
Reverse Primer: 5'-CTCTGCATAGGGCAGACCCA-3' Tm: 59.508
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 137, 133
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr1:24882921-24882973

Rank: 60	Homology Score: 2	TALEN Score: 48.71
	Genomic Region: Intergenic	Closest Gene: NKX6-1
Match Type: R23L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-ACCAG TCGACACCACCCCACATG  CATTTTCTTTTTGATCCTGATCT   TGCATGGGTGACTGATAA TGAAA-3'
	3'-TGGTC AGCTGTGGTGGGGTGTAC  GTAAAAGAAAAACTAGGACTAGA   ACGTACCCACTGACTATT ACTTT-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 004-085429094
Forward Primer: 5'-GTCTCCCTTGCCAGACTATAACC-3' Tm: 59.148
Reverse Primer: 5'-CATGGCCAAGAGCTGTGCTTTC-3' Tm: 59.258
Expected PCR Product Length: 342
Expected CelI Cleavage Product Sizes: 146, 196
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr4:85429209-85429267

Rank: 61	Homology Score: 5	TALEN Score: 48.7
	Genomic Region: Intron	Closest Gene: TMEM9
Match Type: R20L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-GCAGC CACCCTCTTCCCCACATT    CCTTCTCCAACTTCTTAGGT    AGGATTGGGGAAACATAA AGGGC-3'
	3'-CGTCG GTGGGAGAAGGGGTGTAA    GGAAGAGGTTGAAGAATCCA    TCCTAACCCCTTTGTATT TCCCG-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 001-201121271
Forward Primer: 5'-GAGGGATGAGGGGAAATGGG-3' Tm: 59.508
Reverse Primer: 5'-GGAGCTGTTTGGGTAGATCTATGG-3' Tm: 59.038
Expected PCR Product Length: 373
Expected CelI Cleavage Product Sizes: 131, 242
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr1:201121372-201121427

Rank: 62	Homology Score: 5	TALEN Score: 48.65
	Genomic Region: Intron	Closest Gene: LOC100188947
Match Type: R17L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-TCTTC TGCCTGCCACCCCTCAGT     GGAAGCCTGGCAGAGAG      TGGATGGCTGTCAGAAGA TGCTG-3'
	3'-AGAAG ACGGACGGTGGGGAGTCA     CCTTCGGACCGTCTCTC      ACCTACCGACAGTCTTCT ACGAC-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 010-093286041
Forward Primer: 5'-GATTCTGAGCAGGGAGCTTGG-3' Tm: 59.378
Reverse Primer: 5'-ACAGGATGAGCTAGGGCCTGA-3' Tm: 59.378
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 135, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr10:93286148-93286200

Rank: 63	Homology Score: 5	TALEN Score: 48.61
	Genomic Region: Intergenic	Closest Gene: MIR802
Match Type: L21R
	           Left Nuclease
	         TTTTCTGTCACCAATCCT
	5'-CAGAG TCCTTTCCCACCAATCCT   TCATCAGCCTAATCAATACTA    GCAGTGGGGTGGAAGGAA GATGA-3'
	3'-GTCTC AGGAAAGGGTGGTTAGGA   AGTAGTCGGATTAGTTATGAT    CGTCACCCCACCTTCCTT CTACT-5'
	                                                       TGACACCCCACCTCCCCT
	                                                            Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 021-036773953
Forward Primer: 5'-CCAGAGCGGTTTTCCAAGATGC-3' Tm: 59.258
Reverse Primer: 5'-GGATTCACAACACCATGGATGGAC-3' Tm: 59.038
Expected PCR Product Length: 371
Expected CelI Cleavage Product Sizes: 175, 196
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr21:36774098-36774154

Rank: 64	Homology Score: 5	TALEN Score: 48.61
	Genomic Region: Intron	Closest Gene: AFF1
Match Type: L21R
	           Left Nuclease
	         TTTTCTGTCACCAATCCT
	5'-TTCTC TATTCTGCCTCAAAACCT   AGTTGCGGTTGTTCCTTGAGC    ACTGTGGCATGGAGCTGA GTTTC-3'
	3'-AAGAG ATAAGACGGAGTTTTGGA   TCAACGCCAACAAGGAACTCG    TGACACCGTACCTCGACT CAAAG-5'
	                                                       TGACACCCCACCTCCCCT
	                                                            Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 004-088055877
Forward Primer: 5'-TGGGGTGTTTGGATTGGGAGG-3' Tm: 59.378
Reverse Primer: 5'-TCAGCTAGCATTTCAGCTCAGGC-3' Tm: 59.148
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 135, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr4:88055982-88056038

Rank: 65	Homology Score: 2	TALEN Score: 48.59
	Genomic Region: Intron	Closest Gene: SNCA
Match Type: L18R
	           Left Nuclease
	         TTTTCTGTCACCAATCCT
	5'-TTCAA TTTTCTATTATCAATTCC     TAGTCTGCTCATAATGCC     ATTTTGGGGTGGGGAGCA AAGGG-3'
	3'-AAGTT AAAAGATAATAGTTAAGG     ATCAGACGAGTATTACGG     TAAAACCCCACCCCTCGT TTCCC-5'
	                                                       TGACACCCCACCTCCCCT
	                                                            Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 004-090656661
Forward Primer: 5'-CAGGGGAGTGTGTACATAGTGTAG-3' Tm: 59.038
Reverse Primer: 5'-CCAGAGTTGTTCCTCTCCACATAG-3' Tm: 59.038
Expected PCR Product Length: 359
Expected CelI Cleavage Product Sizes: 169, 190
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr4:90656801-90656854

Rank: 66	Homology Score: 2	TALEN Score: 48.48
	Genomic Region: Intergenic	Closest Gene: STIM2
Match Type: R22L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-GTCCC TCCTCTCCTCCCCATTAT   CTGTGGAACATTGACTTCATAT   TGCTTTGGTGACAGATTA TAGGG-3'
	3'-CAGGG AGGAGAGGAGGGGTAATA   GACACCTTGTAACTGAAGTATA   ACGAAACCACTGTCTAAT ATCCC-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 004-027261444
Forward Primer: 5'-GTGGGTTAAGAGAGGTATTTGGCC-3' Tm: 59.038
Reverse Primer: 5'-CCTATCCAAGCCACGATTAACTCC-3' Tm: 59.038
Expected PCR Product Length: 410
Expected CelI Cleavage Product Sizes: 210, 200
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr4:27261623-27261680

Rank: 67	Homology Score: 2	TALEN Score: 48.48
	Genomic Region: Intron	Closest Gene: CPN1
Match Type: R11L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-CCCAC TCCCTCCCACCCCACCTA        CCATGAAGAAT         AGGATTGGGGGCAGGACT GAGCT-3'
	3'-GGGTG AGGGAGGGTGGGGTGGAT        GGTACTTCTTA         TCCTAACCCCCGTCCTGA CTCGA-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 010-101836357
Forward Primer: 5'-CAGCCGCTAATGATCCCAGTTG-3' Tm: 59.258
Reverse Primer: 5'-GACATAGCAGGTGCCTTCCTATTC-3' Tm: 59.038
Expected PCR Product Length: 308
Expected CelI Cleavage Product Sizes: 177, 131
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr10:101836509-101836555

Rank: 68	Homology Score: 2	TALEN Score: 48.44
	Genomic Region: Intron	Closest Gene: TYW1
Match Type: L25R
	           Left Nuclease
	         TTTTCTGTCACCAATCCT
	5'-AAGCT TCTTCTGACACCACTCAA GTGTATGGGGGAGAGGTGCCTTGTT  ACTGCTAGGTGGAGTGAA AGTCC-3'
	3'-TTCGA AGAAGACTGTGGTGAGTT CACATACCCCCTCTCCACGGAACAA  TGACGATCCACCTCACTT TCAGG-5'
	                                                       TGACACCCCACCTCCCCT
	                                                            Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 007-066670329
Forward Primer: 5'-TGACTGTTCCCCCCTTAGACTC-3' Tm: 59.258
Reverse Primer: 5'-TTGACTCCTACTACCAGGGCG-3' Tm: 59.378
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 135, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr7:66670432-66670492

Rank: 69	Homology Score: 5	TALEN Score: 48.42
	Genomic Region: Intron	Closest Gene: STARD7
Match Type: R17L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-CTCCG TGCCCTCCACCTCACCCT     ACCCCCTGCAACATTTA      AAGAAAGGTTACAGATAA GAAAC-3'
	3'-GAGGC ACGGGAGGTGGAGTGGGA     TGGGGGACGTTGTAAAT      TTCTTTCCAATGTCTATT CTTTG-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 002-096866458
Forward Primer: 5'-CGCCGAAACTGGCCTCAAATTCC-3' Tm: 61.088
Reverse Primer: 5'-CTGCCTAGCAGTTACCTTTGTGG-3' Tm: 59.148
Expected PCR Product Length: 295
Expected CelI Cleavage Product Sizes: 130, 165
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr2:96866560-96866612

Rank: 70	Homology Score: 2	TALEN Score: 48.39
	Genomic Region: Intron	Closest Gene: CHD2
Match Type: R19L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-AATAA TACCATCTACCCAACAGG    ATTATTAGAAACTATGCTA     AGATTTTGTGACATTAAA TAAAT-3'
	3'-TTATT ATGGTAGATGGGTTGTCC    TAATAATCTTTGATACGAT     TCTAAAACACTGTAATTT ATTTA-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 015-093472933
Forward Primer: 5'-GGTGTGGATCCCGTTGACAATCT-3' Tm: 59.148
Reverse Primer: 5'-GCCAAGTGCAGGTTTGCTTACAG-3' Tm: 59.148
Expected PCR Product Length: 406
Expected CelI Cleavage Product Sizes: 262, 144
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr15:93473166-93473220

Rank: 71	Homology Score: 2	TALEN Score: 48.35
	Genomic Region: Intergenic	Closest Gene: HERC6
Match Type: R14L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-TGCCC TCCCATCCCCACCCCAGG       GGGCTTCCATGTCA       CAGATAGGTGAGAGATAA ATGGT-3'
	3'-ACGGG AGGGTAGGGGTGGGGTCC       CCCGAAGGTACAGT       GTCTATCCACTCTCTATT TACCA-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 004-089270564
Forward Primer: 5'-GTGGTTGGGGCACAGCTTAG-3' Tm: 59.508
Reverse Primer: 5'-CACACCTGCCTCCCATTCTATTC-3' Tm: 59.148
Expected PCR Product Length: 298
Expected CelI Cleavage Product Sizes: 165, 133
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr4:89270702-89270751

Rank: 72	Homology Score: 5	TALEN Score: 48.34
	Genomic Region: Intergenic	Closest Gene: MOBP
Match Type: L13R
	           Left Nuclease
	         TTTTCTGTCACCAATCCT
	5'-TAGAT TTTTTTGTAAGCAATCTT       TTGAGACACAAGA        AGTGGGGTGTAGAGAGGA ATTCA-3'
	3'-ATCTA AAAAAACATTCGTTAGAA       AACTCTGTGTTCT        TCACCCCACATCTCTCCT TAAGT-5'
	                                                       TGACACCCCACCTCCCCT
	                                                            Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 003-039683322
Forward Primer: 5'-GGTCTCCAGGGAGCATTATACAG-3' Tm: 59.148
Reverse Primer: 5'-CTAGACACAGAGCCTTCAGTAAGC-3' Tm: 59.038
Expected PCR Product Length: 410
Expected CelI Cleavage Product Sizes: 229, 181
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr3:39683525-39683573

Rank: 73	Homology Score: 2	TALEN Score: 48.33
	Genomic Region: Intergenic	Closest Gene: AXIN2
Match Type: L20R
	           Left Nuclease
	         TTTTCTGTCACCAATCCT
	5'-AAAGT TTTTCCATCAACATTTCT    AAATGGAAGAGAGAAGGCAA    AAAGTGGGGTGGGGGTGG GAGGG-3'
	3'-TTTCA AAAAGGTAGTTGTAAAGA    TTTACCTTCTCTCTTCCGTT    TTTCACCCCACCCCCACC CTCCC-5'
	                                                       TGACACCCCACCTCCCCT
	                                                            Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 017-063492561
Forward Primer: 5'-CTCTGTTCCCCACTGATCACC-3' Tm: 59.378
Reverse Primer: 5'-AGCATGGAAGGTCCCCCACA-3' Tm: 59.508
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 135, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr17:63492666-63492721

Rank: 74	Homology Score: 2	TALEN Score: 48.33
	Genomic Region: Intergenic	Closest Gene: NFE2L1
Match Type: R12L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-GAGCC TCTCCACAACTCCCCAGT        CTCCAGTTGGAA        AGGTTTGGTCATATCAAA CAAGA-3'
	3'-CTCGG AGAGGTGTTGAGGGGTCA        GAGGTCAACCTT        TCCAAACCAGTATAGTTT GTTCT-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 017-046139902
Forward Primer: 5'-TGTAACCTGGTTCAGCTCAGTCC-3' Tm: 59.148
Reverse Primer: 5'-ACCGCACCAGGCCTCAAACT-3' Tm: 59.508
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 135, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr17:46140011-46140058

Rank: 75	Homology Score: 2	TALEN Score: 48.31
	Genomic Region: Intergenic	Closest Gene: DIAPH2
Match Type: R11L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-CAAAC TCCCCTCTACCCCCGTGA        TGCTCAGACTA         GGTATTGGTGTCAGGGAA AAAGG-3'
	3'-GTTTG AGGGGAGATGGGGGCACT        ACGAGTCTGAT         CCATAACCACAGTCCCTT TTTCC-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 00X-096909115
Forward Primer: 5'-TAGAGAATGGTGTTTGGGGCTGC-3' Tm: 59.148
Reverse Primer: 5'-GGCTTGTATCTCAAAACTCCCACAGATAAC-3' Tm: 60.058
Expected PCR Product Length: 325
Expected CelI Cleavage Product Sizes: 130, 195
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chrX:96909220-96909266

Rank: 76	Homology Score: 2	TALEN Score: 48.29
	Genomic Region: Intergenic	Closest Gene: CD83
Match Type: L16R
	           Left Nuclease
	         TTTTCTGTCACCAATCCT
	5'-GAAGG TTCTCTGTCCCCTTTCCA      GGGATTCTCAGTAATC      CCTGTGGTGGGGGTGGGA AGGCA-3'
	3'-CTTCC AAGAGACAGGGGAAAGGT      CCCTAAGAGTCATTAG      GGACACCACCCCCACCCT TCCGT-5'
	                                                       TGACACCCCACCTCCCCT
	                                                            Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 006-014315745
Forward Primer: 5'-GGAAGGAAGGAAGGAAGGAGAAAG-3' Tm: 59.038
Reverse Primer: 5'-CCCTGCTTGTTTTTAAGGGGCC-3' Tm: 59.258
Expected PCR Product Length: 379
Expected CelI Cleavage Product Sizes: 215, 164
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr6:14315932-14315983

Rank: 77	Homology Score: 5	TALEN Score: 48.27
	Genomic Region: Intron	Closest Gene: ERC2
Match Type: L11R
	           Left Nuclease
	         TTTTCTGTCACCAATCCT
	5'-TTCAC TTTTCTGGGACAAATGCT        ACCAGAGTGCA         ACTGCTGGGTTGAATGGA AGTTG-3'
	3'-AAGTG AAAAGACCCTGTTTACGA        TGGTCTCACGT         TGACGACCCAACTTACCT TCAAC-5'
	                                                       TGACACCCCACCTCCCCT
	                                                            Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 003-056061208
Forward Primer: 5'-GGCATGGATATCCAGGCTTGCT-3' Tm: 59.258
Reverse Primer: 5'-TGGGGATGTACAGAGGCCAG-3' Tm: 59.508
Expected PCR Product Length: 292
Expected CelI Cleavage Product Sizes: 161, 131
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr3:56061344-56061390

Rank: 78	Homology Score: 2	TALEN Score: 48.25
	Genomic Region: Exon	Closest Gene: HMCN1
Match Type: R23L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-AATTT TTCTCTCCACTCAACAGG  GTGGTGGTGATTGCCCAGAAATG   AGTATTGGAGCTATAAAA ATTGC-3'
	3'-TTAAA AAGAGAGGTGAGTTGTCC  CACCACCACTAACGGGTCTTTAC   TCATAACCTCGATATTTT TAACG-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 001-185833320
Forward Primer: 5'-CATCGTGTAAGAGAAGGCAGAGTG-3' Tm: 59.038
Reverse Primer: 5'-CTGTTGGATAAGTTGCAGCACCTC-3' Tm: 59.038
Expected PCR Product Length: 429
Expected CelI Cleavage Product Sizes: 296, 133
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr1:185833585-185833643

Rank: 79	Homology Score: 2	TALEN Score: 48.25
	Genomic Region: Intergenic	Closest Gene: TBRG4
Match Type: R25L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-TGGAT TCCTCTCGACCCCCAAGC CAGGAGCACAGCCAGGGTGTAGAAA  GTGTTTGGAGAAAGAAAA TCCCA-3'
	3'-ACCTA AGGAGAGCTGGGGGTTCG GTCCTCGTGTCGGTCCCACATCTTT  CACAAACCTCTTTCTTTT AGGGT-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 007-045162226
Forward Primer: 5'-GGGGAGGCTGGTGATTTTTTTGAG-3' Tm: 59.038
Reverse Primer: 5'-GCTGAACACGGTATACAAGGCAAC-3' Tm: 59.038
Expected PCR Product Length: 284
Expected CelI Cleavage Product Sizes: 145, 139
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr7:45162339-45162399

Rank: 80	Homology Score: 2	TALEN Score: 48.17
	Genomic Region: Intron	Closest Gene: GPC4
Match Type: R25L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-TGGAA TCCCCTGCACCCAGCATA CTGCTCTGGATACAGGAGCCATTCA  ATGATTGCTGATAATAAA TTTCT-3'
	3'-ACCTT AGGGGACGTGGGTCGTAT GACGAGACCTATGTCCTCGGTAAGT  TACTAACGACTATTATTT AAAGA-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 00X-132539743
Forward Primer: 5'-CTGCCATAGACTGGGAAACTGGA-3' Tm: 59.148
Reverse Primer: 5'-CCAGAACACAGCTGGGAATGTGGA-3' Tm: 60.908
Expected PCR Product Length: 283
Expected CelI Cleavage Product Sizes: 152, 131
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chrX:132539863-132539923

Rank: 81	Homology Score: 5	TALEN Score: 48.14
	Genomic Region: Intron	Closest Gene: CPED1
Match Type: L12R
	           Left Nuclease
	         TTTTCTGTCACCAATCCT
	5'-CTCCG TTATATGTCATCAAGGCT        GAAGGTTGACAG        ACAGTAGGGTGGAGGAGT GAATG-3'
	3'-GAGGC AATATACAGTAGTTCCGA        CTTCCAACTGTC        TGTCATCCCACCTCCTCA CTTAC-5'
	                                                       TGACACCCCACCTCCCCT
	                                                            Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 007-120742464
Forward Primer: 5'-GGGACAGAATGTGCTCCAAACATG-3' Tm: 59.038
Reverse Primer: 5'-CCCTGTCTTCCTCTAGGGCA-3' Tm: 59.508
Expected PCR Product Length: 325
Expected CelI Cleavage Product Sizes: 164, 161
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr7:120742602-120742649

Rank: 82	Homology Score: 2	TALEN Score: 48.13
	Genomic Region: Promoter	Closest Gene: ODF4
Match Type: R20L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-TCACC TCCCATCACCACCACACT    GGGGAGCAAATTTCAGCATG    AGGTTTGGTGAAGACAAA TTAAC-3'
	3'-AGTGG AGGGTAGTGGTGGTGTGA    CCCCTCGTTTAAAGTCGTAC    TCCAAACCACTTCTGTTT AATTG-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 017-008242168
Forward Primer: 5'-CAGCTATTGCGGGGAGCTAATG-3' Tm: 59.258
Reverse Primer: 5'-AGGCCACAGCTCAGAACTGTGGA-3' Tm: 61.088
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 136, 134
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr17:8242274-8242329

Rank: 83	Homology Score: 2	TALEN Score: 48.12
	Genomic Region: Intron	Closest Gene: CPEB2
Match Type: R22L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-TTTCC TTCCCTGCCCCCAACAAT   TTGTAAAAAGGCTTACTGTGTG   AGTATTTTTGCCAGAAAT TGCTT-3'
	3'-AAAGG AAGGGACGGGGGTTGTTA   AACATTTTTCCGAATGACACAC   TCATAAAAACGGTCTTTA ACGAA-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Warning! Primer pair was generated using suboptimal parameters. 
Primer Index: 004-015022872
Forward Primer: 5'-CCTATTTTCTGGTGACTATCTTGATTCTTGGC-3' Tm: 59.848
Reverse Primer: 5'-CAGATCTTCCCCCAAACCGTTAC-3' Tm: 59.148
Expected PCR Product Length: 411
Expected CelI Cleavage Product Sizes: 264, 147
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr4:15023105-15023162

Rank: 84	Homology Score: 2	TALEN Score: 48.12
	Genomic Region: Intergenic	Closest Gene: UGT8
Match Type: L22R
	           Left Nuclease
	         TTTTCTGTCACCAATCCT
	5'-TATAG TTTTCTTTCAACATTCAC   TCAGGAAATGATTATGTGTGAG   TATGTGGATTTGAGGGGA AGTTA-3'
	3'-ATATC AAAAGAAAGTTGTAAGTG   AGTCCTTTACTAATACACACTC   ATACACCTAAACTCCCCT TCAAT-5'
	                                                       TGACACCCCACCTCCCCT
	                                                            Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 004-115491385
Forward Primer: 5'-CATGTTGCACAGGCTGAGTACTCT-3' Tm: 59.038
Reverse Primer: 5'-CCCATAGCTCTTTGGTCAGGTC-3' Tm: 59.258
Expected PCR Product Length: 399
Expected CelI Cleavage Product Sizes: 224, 175
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr4:115491578-115491635

Rank: 85	Homology Score: 2	TALEN Score: 48.11
	Genomic Region: Intergenic	Closest Gene: SUCLG1
Match Type: L13R
	           Left Nuclease
	         TTTTCTGTCACCAATCCT
	5'-AATGA GCTTCTGCCTCTAATCCT       GATGATGATAATG        ATGATGGTGTGGTGGGGA TGGTA-3'
	3'-TTACT CGAAGACGGAGATTAGGA       CTACTACTATTAC        TACTACCACACCACCCCT ACCAT-5'
	                                                       TGACACCCCACCTCCCCT
	                                                            Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 002-084649832
Forward Primer: 5'-AAAGAATGTGGGCTCTGGATGGG-3' Tm: 59.148
Reverse Primer: 5'-GAGGCTGTAACAGTGTCAACGTG-3' Tm: 59.148
Expected PCR Product Length: 352
Expected CelI Cleavage Product Sizes: 130, 222
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr2:84649936-84649984

Rank: 86	Homology Score: 2	TALEN Score: 48.09
	Genomic Region: Intergenic	Closest Gene: FAM9C
Match Type: R22L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-GAATT TCCCTTCTATCCCCCAGA   TCCACACTTCTCTATTCAAGTC   AGGGTTTGTGACAGGGTA GCCTA-3'
	3'-CTTAA AGGGAAGATAGGGGGTCT   AGGTGTGAAGAGATAAGTTCAG   TCCCAAACACTGTCCCAT CGGAT-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 00X-013108270
Forward Primer: 5'-GCCATTTTGGCAGAGCCACTGA-3' Tm: 59.258
Reverse Primer: 5'-GCATGTTGTGCACGAAATGACCTC-3' Tm: 59.038
Expected PCR Product Length: 271
Expected CelI Cleavage Product Sizes: 131, 140
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chrX:13108370-13108427

Rank: 87	Homology Score: 2	TALEN Score: 48.08
	Genomic Region: Intron	Closest Gene: ANGPT1
Match Type: R12L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-ATCTC TTCCCTGCTCCCCACCAT        TCAGAATTTGTT        GTAATTTGTGAAAGAAAA GTGAA-3'
	3'-TAGAG AAGGGACGAGGGGTGGTA        AGTCTTAAACAA        CATTAAACACTTTCTTTT CACTT-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 008-108496180
Forward Primer: 5'-GATGGGAAAGATGCCTAGGAAGC-3' Tm: 59.148
Reverse Primer: 5'-GGAAACTGAGGGCCACTAAAGTC-3' Tm: 59.148
Expected PCR Product Length: 339
Expected CelI Cleavage Product Sizes: 186, 153
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr8:108496340-108496387

Rank: 88	Homology Score: 2	TALEN Score: 48.08
	Genomic Region: Intron	Closest Gene: KIAA0556
Match Type: L21R
	           Left Nuclease
	         TTTTCTGTCACCAATCCT
	5'-GGACC CATTCAGTGACCACTCCT   ATAGTTCCCTAGTTCACTTGT    CTTGTGGGGGTGAGAGGA GGGAC-3'
	3'-CCTGG GTAAGTCACTGGTGAGGA   TATCAAGGGATCAAGTGAACA    GAACACCCCCACTCTCCT CCCTG-5'
	                                                       TGACACCCCACCTCCCCT
	                                                            Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 016-027592598
Forward Primer: 5'-CCCTGTGCACATAGGAATAGCC-3' Tm: 59.258
Reverse Primer: 5'-GGGTGATGGGTGCATCAATATCTC-3' Tm: 59.038
Expected PCR Product Length: 416
Expected CelI Cleavage Product Sizes: 241, 175
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr16:27592809-27592865

Rank: 89	Homology Score: 5	TALEN Score: 48.06
	Genomic Region: Intergenic	Closest Gene: NUF2
Match Type: R11L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-TTGCT TGACCTCCACACCACACT        CCATCTCTCTT         GGGATAAGTGCCAAAAAA GAGGT-3'
	3'-AACGA ACTGGAGGTGTGGTGTGA        GGTAGAGAGAA         CCCTATTCACGGTTTTTT CTCCA-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 001-163803301
Forward Primer: 5'-GGTTAAGTGCTTGACCACTGCTG-3' Tm: 59.148
Reverse Primer: 5'-CACCATGGCACATGTCTACTTGAG-3' Tm: 59.038
Expected PCR Product Length: 336
Expected CelI Cleavage Product Sizes: 139, 197
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr1:163803415-163803461

Rank: 90	Homology Score: 2	TALEN Score: 48.05
	Genomic Region: Intergenic	Closest Gene: PLCXD3
Match Type: L12R
	           Left Nuclease
	         TTTTCTGTCACCAATCCT
	5'-AATTC ATTTCTGTCTCCAGGCCA        TAGATGAGAGAG        AGGGAGAGGGGGAGGGGA ATAGG-3'
	3'-TTAAG TAAAGACAGAGGTCCGGT        ATCTACTCTCTC        TCCCTCTCCCCCTCCCCT TATCC-5'
	                                                       TGACACCCCACCTCCCCT
	                                                            Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 005-041303604
Forward Primer: 5'-GTCTTGAGAGAATGAGAAGCCAGG-3' Tm: 59.038
Reverse Primer: 5'-CTCCTGGTCCCAAGTGATCC-3' Tm: 59.508
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 136, 134
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr5:41303714-41303761

Rank: 91	Homology Score: 2	TALEN Score: 48.02
	Genomic Region: Intron	Closest Gene: SLC9A2
Match Type: L25R
	           Left Nuclease
	         TTTTCTGTCACCAATCCT
	5'-TTTCC TTCTCTGACACCACCCCA ATAGAAAAGCCAGGGTGTTCCTTAC  ACTCTGGTGAGGATGGAA TCTAA-3'
	3'-AAAGG AAGAGACTGTGGTGGGGT TATCTTTTCGGTCCCACAAGGAATG  TGAGACCACTCCTACCTT AGATT-5'
	                                                       TGACACCCCACCTCCCCT
	                                                            Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 002-103259635
Forward Primer: 5'-GTTCTCTGCGTTCTTTACGTCGTC-3' Tm: 59.038
Reverse Primer: 5'-GTCAACCTCACAAGGCACAGAAG-3' Tm: 59.148
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 130, 140
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr2:103259733-103259793

Rank: 92	Homology Score: 2	TALEN Score: 47.96
	Genomic Region: Intergenic	Closest Gene: TSPAN8
Match Type: L11R
	           Left Nuclease
	         TTTTCTGTCACCAATCCT
	5'-TGAAT TACTCTTTCACCAACCCA        CTTAGTGACCA         ACTATGGTTTGAAGGAGA CTGCA-3'
	3'-ACTTA ATGAGAAAGTGGTTGGGT        GAATCACTGGT         TGATACCAAACTTCCTCT GACGT-5'
	                                                       TGACACCCCACCTCCCCT
	                                                            Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 012-071464141
Forward Primer: 5'-CCACAAGTTTGGGGAGCAAGAC-3' Tm: 59.258
Reverse Primer: 5'-AAGCAAATGCCTAGTGTGGGAAGC-3' Tm: 59.038
Expected PCR Product Length: 290
Expected CelI Cleavage Product Sizes: 159, 131
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr12:71464275-71464321

Rank: 93	Homology Score: 2	TALEN Score: 47.95
	Genomic Region: Intron	Closest Gene: LGR6
Match Type: R22L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-GATGT CCCCCTCCACTCCAAAAG   GCACAGGCTGTGGGCCAGGTAA   GGGAGTGGGAAGAGAAAA ATAAT-3'
	3'-CTACA GGGGGAGGTGAGGTTTTC   CGTGTCCGACACCCGGTCCATT   CCCTCACCCTTCTCTTTT TATTA-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 001-202164461
Forward Primer: 5'-TGCTCAGTGTTGGGAGTGTCCT-3' Tm: 59.258
Reverse Primer: 5'-TTTGCCAGATGCTGAGCGCC-3' Tm: 59.508
Expected PCR Product Length: 276
Expected CelI Cleavage Product Sizes: 131, 145
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr1:202164561-202164618

Rank: 94	Homology Score: 2	TALEN Score: 47.95
	Genomic Region: Intergenic	Closest Gene: USP9X
Match Type: L26R
	           Left Nuclease
	         TTTTCTGTCACCAATCCT
	5'-TCCAC TCTTCTGACACCACCCCA GAGAGGAGGGGGAGGAGTACTTTGTT ACTGCCTGGTGGAGGTGG ACATC-3'
	3'-AGGTG AGAAGACTGTGGTGGGGT CTCTCCTCCCCCTCCTCATGAAACAA TGACGGACCACCTCCACC TGTAG-5'
	                                                       TGACACCCCACCTCCCCT
	                                                            Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 00X-040907277
Forward Primer: 5'-CTGGTTAGGAGGGTTGAAGGTG-3' Tm: 59.258
Reverse Primer: 5'-GGTCAGAGAAGGCTGAGTAGG-3' Tm: 59.378
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 137, 133
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chrX:40907381-40907442

Rank: 95	Homology Score: 2	TALEN Score: 47.95
	Genomic Region: Intergenic	Closest Gene: FLJ25363
Match Type: L21R
	           Left Nuclease
	         TTTTCTGTCACCAATCCT
	5'-TTTAA TTTCCTTTCAGCAAACAT   TTATTGAGTGCCAAGCATTGA    ACTAGGTGTTGGAAGGGA ATACA-3'
	3'-AAATT AAAGGAAAGTCGTTTGTA   AATAACTCACGGTTCGTAACT    TGATCCACAACCTTCCCT TATGT-5'
	                                                       TGACACCCCACCTCCCCT
	                                                            Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 003-109619335
Forward Primer: 5'-CAGGGAAGTCTCAGTGGTTTTTGG-3' Tm: 59.038
Reverse Primer: 5'-CCAGGTGTATTCTGTGTGGCTAG-3' Tm: 59.148
Expected PCR Product Length: 421
Expected CelI Cleavage Product Sizes: 266, 155
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr3:109619571-109619627

Rank: 96	Homology Score: 2	TALEN Score: 47.93
	Genomic Region: Intron	Closest Gene: ANKRD28
Match Type: L22R
	           Left Nuclease
	         TTTTCTGTCACCAATCCT
	5'-GACCA TCCTCTGAGACCCATCCT   TTCTCAGAGGCTGGGAAGGGTA   GTTGGGGGATGGAGGGGT GGGGA-3'
	3'-CTGGT AGGAGACTCTGGGTAGGA   AAGAGTCTCCGACCCTTCCCAT   CAACCCCCTACCTCCCCA CCCCT-5'
	                                                       TGACACCCCACCTCCCCT
	                                                            Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 003-015883800
Forward Primer: 5'-GCCAGGCACAAAAAGACAAAGTTTGCATG-3' Tm: 60.178
Reverse Primer: 5'-GGGGTATCCATCACCTCAAGC-3' Tm: 59.378
Expected PCR Product Length: 342
Expected CelI Cleavage Product Sizes: 141, 201
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr3:15883910-15883967

Rank: 97	Homology Score: 2	TALEN Score: 47.92
	Genomic Region: Intron	Closest Gene: ADCK2
Match Type: R24L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-TGGGC CCGTCTCCAGCCCACAGA  AAGGCTTCAGTCTGGAGAGAGGAT  GGGATTTGTGTCTGTAAA ACTGT-3'
	3'-ACCCG GGCAGAGGTCGGGTGTCT  TTCCGAAGTCAGACCTCTCTCCTA  CCCTAAACACAGACATTT TGACA-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 007-140379038
Forward Primer: 5'-TCTGCGCCCCTTTGTCACCA-3' Tm: 59.508
Reverse Primer: 5'-CCTCTAAGGCAGGTCACCAAC-3' Tm: 59.378
Expected PCR Product Length: 299
Expected CelI Cleavage Product Sizes: 132, 167
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr7:140379138-140379197

Rank: 98	Homology Score: 2	TALEN Score: 47.9
	Genomic Region: Intron	Closest Gene: C16orf46
Match Type: L17R
	           Left Nuclease
	         TTTTCTGTCACCAATCCT
	5'-GTTTA TTTTCTTTTACCAATAAC     ATGTTTTGCTTCAAAAT      AATGTGAGGTTGAGGTTA GGGAG-3'
	3'-CAAAT AAAAGAAAATGGTTATTG     TACAAAACGAAGTTTTA      TTACACTCCAACTCCAAT CCCTC-5'
	                                                       TGACACCCCACCTCCCCT
	                                                            Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 016-081100645
Forward Primer: 5'-GCCCCCAAAGTGCATGTTTGGA-3' Tm: 59.258
Reverse Primer: 5'-GAGCTCTCCGATAACATCATGGC-3' Tm: 59.148
Expected PCR Product Length: 420
Expected CelI Cleavage Product Sizes: 244, 176
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr16:81100861-81100913

Rank: 99	Homology Score: 2	TALEN Score: 47.88
	Genomic Region: Intergenic	Closest Gene: ADCK2
Match Type: R22L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-TCTAG TCCCCTGCATCCCGCAAC   ATTTCTTGGCGAGCCAGCCAGG   AGGAGTGGAGACGGCAGA TCTAC-3'
	3'-AGATC AGGGGACGTAGGGCGTTG   TAAAGAACCGCTCGGTCGGTCC   TCCTCACCTCTGCCGTCT AGATG-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 007-140355184
Forward Primer: 5'-CCCACAAGGGCCTTATTTTCAGTC-3' Tm: 59.038
Reverse Primer: 5'-GAGAGCTGATTTCACCCCTCTAG-3' Tm: 59.148
Expected PCR Product Length: 369
Expected CelI Cleavage Product Sizes: 236, 133
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr7:140355389-140355446

Rank: 100	Homology Score: 2	TALEN Score: 47.88
	Genomic Region: Intergenic	Closest Gene: TAS2R42
Match Type: L16R
	           Left Nuclease
	         TTTTCTGTCACCAATCCT
	5'-GTATT TTTTCTTTTTCAAAACCT      ATGGGCTGTACTTTTC      CATGTGGGGTGATGGGTA ACAAG-3'
	3'-CATAA AAAAGAAAAAGTTTTGGA      TACCCGACATGAAAAG      GTACACCCCACTACCCAT TGTTC-5'
	                                                       TGACACCCCACCTCCCCT
	                                                            Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 012-011342495
Forward Primer: 5'-CTGTCTTCCAGTCTAAAGCAACTACCTC-3' Tm: 60.298
Reverse Primer: 5'-CCACACATCCTCTTCCTCCTATTC-3' Tm: 59.038
Expected PCR Product Length: 385
Expected CelI Cleavage Product Sizes: 132, 253
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr12:11342599-11342650

Plate format Oligo Order Form
Well
Name
Sequence
5' mods
Internal
Notes
Primer Plate #1
A1
019-055627003F
CCAGGAAATGGGGGTGTGTC
Hockemeyer_AAVS1_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
A2
019-055627003R
GTCCACTTCAGGACAGCATGTTTG
Hockemeyer_AAVS1_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
B1
007-115754830F
CGGTTGAGACTGAAGCATGCAAAG
Hockemeyer_AAVS1_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
B2
007-115754830R
AAGGACCACACTACTGACATTAGATCTACC
Hockemeyer_AAVS1_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
C1
001-024697363F
AGCCTCCCAAAGTGCTAGGAC
Hockemeyer_AAVS1_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
C2
001-024697363R
GTGGAGAACATCCATTCACAGGG
Hockemeyer_AAVS1_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
D1
013-103511881F
TCTCTGGCTGGCAGTTGAGG
Hockemeyer_AAVS1_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
D2
013-103511881R
CAGGTCACTGAAAACTGCAAACCG
Hockemeyer_AAVS1_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
E1
017-035084330F
GAGCCTCTGCTGGTTGGATG
Hockemeyer_AAVS1_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
E2
017-035084330R
ATGTTGGGTGCTGGGGAAGG
Hockemeyer_AAVS1_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
F1
016-009446101F
ATCCTTCCTCTGGGGGCACT
Hockemeyer_AAVS1_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
F2
016-009446101R
CTCCAACCTCCCAATGAAACACC
Hockemeyer_AAVS1_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
G1
00X-031479613F
GTCTGGACATGTCTGAATGCCTAG
Hockemeyer_AAVS1_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
G2
00X-031479613R
GGTTAGGTAAAGTGTCCCCTGC
Hockemeyer_AAVS1_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
H1
011-109627678F
TCCTCCTTGGAACCCCAGATTTG
Hockemeyer_AAVS1_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
H2
011-109627678R
TGTCTTCACTGCTGCACATTCCC
Hockemeyer_AAVS1_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
A3
020-025439956F
AGTCCCTGCACTGACTCAGC
Hockemeyer_AAVS1_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
A4
020-025439956R
GTGCTTGATGCGTGGGAGTC
Hockemeyer_AAVS1_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
B3
012-047346370F
CTTGGTATGTTCCAGTGTGGCC
Hockemeyer_AAVS1_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
B4
012-047346370R
GGATGTGTGCAGGATGTACAGG
Hockemeyer_AAVS1_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
C3
004-148942680F
GTAGGTAAGCCAGGGAGCTGT
Hockemeyer_AAVS1_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
C4
004-148942680R
CCTGCCAACACCATGTTTCCAC
Hockemeyer_AAVS1_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
D3
011-011075984F
GCCTCATTACTGCTAGGTGGAAG
Hockemeyer_AAVS1_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
D4
011-011075984R
CACCCTAACATCCAGGCATGATGA
Hockemeyer_AAVS1_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
E3
003-124702567F
TGCAAGTCTACAACCAGGGTGTG
Hockemeyer_AAVS1_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
E4
003-124702567R
CAGAAGGCAGCTGGAAGTCTC
Hockemeyer_AAVS1_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
F3
001-062633493F
TCCTCCTGCCTAATGGCTTTACTG
Hockemeyer_AAVS1_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
F4
001-062633493R
GCTACTGAGCCCAGCCTAATTTAC
Hockemeyer_AAVS1_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
G3
020-058618528F
GGGAGCTATGATCATGCCATAGG
Hockemeyer_AAVS1_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
G4
020-058618528R
GCTCCATGTCATTGTTCTGGGC
Hockemeyer_AAVS1_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
H3
005-157580667F
GTAATCCAGGGTCTCTGTTTGTCC
Hockemeyer_AAVS1_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
H4
005-157580667R
GTCTTTGGGTGGCATTTGTGAGG
Hockemeyer_AAVS1_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
A5
002-001827851F
GGGGGTTGGGGAACGTTTTTAG
Hockemeyer_AAVS1_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
A6
002-001827851R
TCAGGACGAGAGCAGCTCAAATG
Hockemeyer_AAVS1_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
B5
010-096170810F
GGCATGCCAGAGATAGGACTATTC
Hockemeyer_AAVS1_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
B6
010-096170810R
GTGCTACAGTGGCAACCTTCATG
Hockemeyer_AAVS1_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
C5
020-050029460F
AGAAGCACAGGATGAGGCCG
Hockemeyer_AAVS1_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
C6
020-050029460R
CAGTGGATTGACAGAGGCAGATG
Hockemeyer_AAVS1_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
D5
003-051729704F
GGAGATGAACTCAGGCTGAAGTG
Hockemeyer_AAVS1_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
D6
003-051729704R
TTCCCTCCCACCTGGGTCTT
Hockemeyer_AAVS1_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
E5
015-067305574F
CCCCACCTTGCTGAATGGAC
Hockemeyer_AAVS1_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
E6
015-067305574R
CCAGTCCTTGATCTCTTCCTGAGT
Hockemeyer_AAVS1_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
F5
011-060790178F
GCCTGTGCTGAGACTGGGAA
Hockemeyer_AAVS1_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
F6
011-060790178R
AGCTGTCAGTCAAATTGCTGGGTG
Hockemeyer_AAVS1_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
G5
003-070995805F
GCCGTTGCTTTTGCCTTGAACATC
Hockemeyer_AAVS1_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
G6
003-070995805R
TACTTGAGTCCCTGGATCTGGC
Hockemeyer_AAVS1_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
H5
00X-018438373F
CAGGTCATGACCATCAGTGATCAG
Hockemeyer_AAVS1_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
H6
00X-018438373R
CACCTGTCAGGCTTGTGGCT
Hockemeyer_AAVS1_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
A7
012-030180063F
CTATAAGGCTGCTGGGCCTTTC
Hockemeyer_AAVS1_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
A8
012-030180063R
GAGGCAAACCTACTTTATGGAGGG
Hockemeyer_AAVS1_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
B7
004-146944903F
CAGAGGAGTTCAGCTCAGTTTTGC
Hockemeyer_AAVS1_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
B8
004-146944903R
CCCTCCCCCAAGGACAACAA
Hockemeyer_AAVS1_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
C7
001-178005473F
CTCACAGAAGTGCGAACCGTATTG
Hockemeyer_AAVS1_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
C8
001-178005473R
CACCAATATGGCCACCTCTGTAC
Hockemeyer_AAVS1_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
D7
006-099703892F
GGACTGCATAAACCACTTGAGCC
Hockemeyer_AAVS1_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
D8
006-099703892R
GCAGGACTCTCTAGCTGGCTAT
Hockemeyer_AAVS1_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
E7
016-024267933F
CAAAGTCCCTGTGGATGCTGACAA
Hockemeyer_AAVS1_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
E8
016-024267933R
TAGTCCTGCACACACCTCTGG
Hockemeyer_AAVS1_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
F7
008-107822970F
GGCTAAGTGTGAGAATCGAGTGAG
Hockemeyer_AAVS1_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
F8
008-107822970R
GTTAGTGAGACAGCATGTCCCTTC
Hockemeyer_AAVS1_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
G7
002-154289086F
GGTTTCAAGTTTCACCTATTGCTTGGCAG
Hockemeyer_AAVS1_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
G8
002-154289086R
CGTGTTATGTGTGTTTAGCCCCC
Hockemeyer_AAVS1_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
H7
007-052309823F
CCTGAGTCCCTGCTGCATTAATTC
Hockemeyer_AAVS1_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
H8
007-052309823R
ACTCTGTGTCCTCAACGGTGTTC
Hockemeyer_AAVS1_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
A9
008-145493530F
GAGCCATTAAGGGCTAGAGGG
Hockemeyer_AAVS1_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
A10
008-145493530R
GGAAAGCTCCCAAGAAGGGAAG
Hockemeyer_AAVS1_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
B9
008-145324445F
GAGCCATTAAGGGCTAGAGGG
Hockemeyer_AAVS1_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
B10
008-145324445R
GGAAAGCTCCCAAGAAGGGAAG
Hockemeyer_AAVS1_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
C9
005-127879056F
CTCCTGGATAGTGTCAGAGAAAGC
Hockemeyer_AAVS1_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
C10
005-127879056R
CTGCTCCCAATGTAGTTTCCCATG
Hockemeyer_AAVS1_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
D9
006-011853744F
GTCTACGGATCTAACACTCCAAAAATCTTAGC
Hockemeyer_AAVS1_Ranking-TALENv2.0	Warning! Primer pair was generated using suboptimal parameters. 
D10
006-011853744R
CTAGGGGGCTGAAATGGAACAAC
Hockemeyer_AAVS1_Ranking-TALENv2.0	Warning! Primer pair was generated using suboptimal parameters. 
E9
012-003414995F
TGACGTTCCCTGGGCCAAGA
Hockemeyer_AAVS1_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
E10
012-003414995R
AAAATCCAGTTCCTTGCTCGCACC
Hockemeyer_AAVS1_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
F9
001-114585589F
CCCCATAAATGATCTGCATCCAGG
Hockemeyer_AAVS1_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
F10
001-114585589R
CGCTGAGAAGGCCAGATGAG
Hockemeyer_AAVS1_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
G9
007-158192599F
TAGTCCCCAGGCACTTGCAG
Hockemeyer_AAVS1_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
G10
007-158192599R
GCCTCGTAAGTTTAGGCATCCTC
Hockemeyer_AAVS1_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
H9
006-168932997F
TCCTTCGTCCTGGGATTGGG
Hockemeyer_AAVS1_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
H10
006-168932997R
GGGTGCACAGTTACAGAGGAG
Hockemeyer_AAVS1_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
A11
019-038858026F
TTCCTCTCCCCGCAGACTAC
Hockemeyer_AAVS1_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
A12
019-038858026R
AGTGCACACAGACTGGCCTC
Hockemeyer_AAVS1_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
B11
00X-085476489F
GGAAATGTGAGGCCATCAGCTTTG
Hockemeyer_AAVS1_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
B12
00X-085476489R
CAGCCCCCAGAACTGTGAGA
Hockemeyer_AAVS1_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
C11
011-126543310F
CCAGCAGCTCTGGAAATTGGATC
Hockemeyer_AAVS1_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
C12
011-126543310R
GAGGAAGATCTTACAGACCCAACC
Hockemeyer_AAVS1_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
D11
00X-074326678F
CCCTTCCCTTCAAGGACACC
Hockemeyer_AAVS1_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
D12
00X-074326678R
GCTGAGTTTCGTCCTGTTTCTGG
Hockemeyer_AAVS1_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
E11
020-050472318F
CTGGGTCATGTGGTACTGCATGT
Hockemeyer_AAVS1_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
E12
020-050472318R
CTGCAGTTGATCCATCCAGACC
Hockemeyer_AAVS1_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
F11
006-092651079F
CAGCAAGGAGGCCAAGAACTCA
Hockemeyer_AAVS1_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
F12
006-092651079R
GTGTGTGGGCCTCAAGTTTTGTAC
Hockemeyer_AAVS1_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
G11
011-119574363F
CAACGAGGCTGACGCTCTTTC
Hockemeyer_AAVS1_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
G12
011-119574363R
CTGAGCACCTGTGTGCCAGT
Hockemeyer_AAVS1_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
H11
002-149982161F
GGCATCTTCGAGTACCTTAACTCC
Hockemeyer_AAVS1_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
H12
002-149982161R
CTCTTCTCAAGTTCAAGGGTCAGTTGTAC
Hockemeyer_AAVS1_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
Primer Plate #2
A1
011-057225300F
AGCCTCACCGTACAGATGAGGA
Hockemeyer_AAVS1_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
A2
011-057225300R
TTTAGGTGGCTGGCTGGTGG
Hockemeyer_AAVS1_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
B1
001-198360906F
GGTTTTATAGCCCTTCTCCTGCC
Hockemeyer_AAVS1_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
B2
001-198360906R
GTTGTGGGATTTGAGCTGGTGG
Hockemeyer_AAVS1_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
C1
011-040808649F
CCCATGTCTCAGTCTCTGTTCTAC
Hockemeyer_AAVS1_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
C2
011-040808649R
CTCACTTGTCAGCAAGCACAAAGG
Hockemeyer_AAVS1_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
D1
011-008291463F
GTTTCCTCGGGCAATGAATGAGC
Hockemeyer_AAVS1_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
D2
011-008291463R
GCACAGTCCTCCTTTCTTCACC
Hockemeyer_AAVS1_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
E1
006-021198081F
CCGTGATTTGAACAGGGTTCGC
Hockemeyer_AAVS1_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
E2
006-021198081R
CTCTCCCCTTTAAATGCCCTTTGC
Hockemeyer_AAVS1_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
F1
002-064716657F
GAAATGCATCCCCTCTAGCCTG
Hockemeyer_AAVS1_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
F2
002-064716657R
GCTGCATGGACAGTGCTCACA
Hockemeyer_AAVS1_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
G1
006-021174562F
GTCGGTATAATCTTAGTCAAAAGTTCAACAGGG
Hockemeyer_AAVS1_Ranking-TALENv2.0	Warning! Primer pair was generated using suboptimal parameters. 
G2
006-021174562R
TAGTTAAATTGCCCTCCAAAATGGCCTTATCAATG
Hockemeyer_AAVS1_Ranking-TALENv2.0	Warning! Primer pair was generated using suboptimal parameters. 
H1
009-129555142F
TTGACCCCTTCATGCCCCCT
Hockemeyer_AAVS1_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
H2
009-129555142R
CCTCTTCCTGGAGCAGGGAT
Hockemeyer_AAVS1_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
A3
010-024751913F
GGTGGATGTGCAGAGATGGAG
Hockemeyer_AAVS1_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
A4
010-024751913R
CTCTACTGAGCCCTCCAGTG
Hockemeyer_AAVS1_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
B3
002-207954053F
GTTTTAGCCACAGGGAAGCTGC
Hockemeyer_AAVS1_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
B4
002-207954053R
GAAGCTGATCCCAAGAAGTTTGGC
Hockemeyer_AAVS1_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
C3
001-024882812F
CATCTGTTGGAGGAAGTGCCTG
Hockemeyer_AAVS1_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
C4
001-024882812R
CTCTGCATAGGGCAGACCCA
Hockemeyer_AAVS1_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
D3
004-085429094F
GTCTCCCTTGCCAGACTATAACC
Hockemeyer_AAVS1_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
D4
004-085429094R
CATGGCCAAGAGCTGTGCTTTC
Hockemeyer_AAVS1_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
E3
001-201121271F
GAGGGATGAGGGGAAATGGG
Hockemeyer_AAVS1_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
E4
001-201121271R
GGAGCTGTTTGGGTAGATCTATGG
Hockemeyer_AAVS1_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
F3
010-093286041F
GATTCTGAGCAGGGAGCTTGG
Hockemeyer_AAVS1_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
F4
010-093286041R
ACAGGATGAGCTAGGGCCTGA
Hockemeyer_AAVS1_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
G3
021-036773953F
CCAGAGCGGTTTTCCAAGATGC
Hockemeyer_AAVS1_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
G4
021-036773953R
GGATTCACAACACCATGGATGGAC
Hockemeyer_AAVS1_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
H3
004-088055877F
TGGGGTGTTTGGATTGGGAGG
Hockemeyer_AAVS1_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
H4
004-088055877R
TCAGCTAGCATTTCAGCTCAGGC
Hockemeyer_AAVS1_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
A5
004-090656661F
CAGGGGAGTGTGTACATAGTGTAG
Hockemeyer_AAVS1_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
A6
004-090656661R
CCAGAGTTGTTCCTCTCCACATAG
Hockemeyer_AAVS1_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
B5
004-027261444F
GTGGGTTAAGAGAGGTATTTGGCC
Hockemeyer_AAVS1_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
B6
004-027261444R
CCTATCCAAGCCACGATTAACTCC
Hockemeyer_AAVS1_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
C5
010-101836357F
CAGCCGCTAATGATCCCAGTTG
Hockemeyer_AAVS1_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
C6
010-101836357R
GACATAGCAGGTGCCTTCCTATTC
Hockemeyer_AAVS1_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
D5
007-066670329F
TGACTGTTCCCCCCTTAGACTC
Hockemeyer_AAVS1_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
D6
007-066670329R
TTGACTCCTACTACCAGGGCG
Hockemeyer_AAVS1_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
E5
002-096866458F
CGCCGAAACTGGCCTCAAATTCC
Hockemeyer_AAVS1_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
E6
002-096866458R
CTGCCTAGCAGTTACCTTTGTGG
Hockemeyer_AAVS1_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
F5
015-093472933F
GGTGTGGATCCCGTTGACAATCT
Hockemeyer_AAVS1_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
F6
015-093472933R
GCCAAGTGCAGGTTTGCTTACAG
Hockemeyer_AAVS1_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
G5
004-089270564F
GTGGTTGGGGCACAGCTTAG
Hockemeyer_AAVS1_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
G6
004-089270564R
CACACCTGCCTCCCATTCTATTC
Hockemeyer_AAVS1_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
H5
003-039683322F
GGTCTCCAGGGAGCATTATACAG
Hockemeyer_AAVS1_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
H6
003-039683322R
CTAGACACAGAGCCTTCAGTAAGC
Hockemeyer_AAVS1_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
A7
017-063492561F
CTCTGTTCCCCACTGATCACC
Hockemeyer_AAVS1_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
A8
017-063492561R
AGCATGGAAGGTCCCCCACA
Hockemeyer_AAVS1_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
B7
017-046139902F
TGTAACCTGGTTCAGCTCAGTCC
Hockemeyer_AAVS1_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
B8
017-046139902R
ACCGCACCAGGCCTCAAACT
Hockemeyer_AAVS1_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
C7
00X-096909115F
TAGAGAATGGTGTTTGGGGCTGC
Hockemeyer_AAVS1_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
C8
00X-096909115R
GGCTTGTATCTCAAAACTCCCACAGATAAC
Hockemeyer_AAVS1_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
D7
006-014315745F
GGAAGGAAGGAAGGAAGGAGAAAG
Hockemeyer_AAVS1_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
D8
006-014315745R
CCCTGCTTGTTTTTAAGGGGCC
Hockemeyer_AAVS1_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
E7
003-056061208F
GGCATGGATATCCAGGCTTGCT
Hockemeyer_AAVS1_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
E8
003-056061208R
TGGGGATGTACAGAGGCCAG
Hockemeyer_AAVS1_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
F7
001-185833320F
CATCGTGTAAGAGAAGGCAGAGTG
Hockemeyer_AAVS1_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
F8
001-185833320R
CTGTTGGATAAGTTGCAGCACCTC
Hockemeyer_AAVS1_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
G7
007-045162226F
GGGGAGGCTGGTGATTTTTTTGAG
Hockemeyer_AAVS1_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
G8
007-045162226R
GCTGAACACGGTATACAAGGCAAC
Hockemeyer_AAVS1_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
H7
00X-132539743F
CTGCCATAGACTGGGAAACTGGA
Hockemeyer_AAVS1_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
H8
00X-132539743R
CCAGAACACAGCTGGGAATGTGGA
Hockemeyer_AAVS1_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
A9
007-120742464F
GGGACAGAATGTGCTCCAAACATG
Hockemeyer_AAVS1_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
A10
007-120742464R
CCCTGTCTTCCTCTAGGGCA
Hockemeyer_AAVS1_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
B9
017-008242168F
CAGCTATTGCGGGGAGCTAATG
Hockemeyer_AAVS1_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
B10
017-008242168R
AGGCCACAGCTCAGAACTGTGGA
Hockemeyer_AAVS1_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
C9
004-015022872F
CCTATTTTCTGGTGACTATCTTGATTCTTGGC
Hockemeyer_AAVS1_Ranking-TALENv2.0	Warning! Primer pair was generated using suboptimal parameters. 
C10
004-015022872R
CAGATCTTCCCCCAAACCGTTAC
Hockemeyer_AAVS1_Ranking-TALENv2.0	Warning! Primer pair was generated using suboptimal parameters. 
D9
004-115491385F
CATGTTGCACAGGCTGAGTACTCT
Hockemeyer_AAVS1_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
D10
004-115491385R
CCCATAGCTCTTTGGTCAGGTC
Hockemeyer_AAVS1_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
E9
002-084649832F
AAAGAATGTGGGCTCTGGATGGG
Hockemeyer_AAVS1_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
E10
002-084649832R
GAGGCTGTAACAGTGTCAACGTG
Hockemeyer_AAVS1_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
F9
00X-013108270F
GCCATTTTGGCAGAGCCACTGA
Hockemeyer_AAVS1_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
F10
00X-013108270R
GCATGTTGTGCACGAAATGACCTC
Hockemeyer_AAVS1_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
G9
008-108496180F
GATGGGAAAGATGCCTAGGAAGC
Hockemeyer_AAVS1_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
G10
008-108496180R
GGAAACTGAGGGCCACTAAAGTC
Hockemeyer_AAVS1_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
H9
016-027592598F
CCCTGTGCACATAGGAATAGCC
Hockemeyer_AAVS1_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
H10
016-027592598R
GGGTGATGGGTGCATCAATATCTC
Hockemeyer_AAVS1_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
A11
001-163803301F
GGTTAAGTGCTTGACCACTGCTG
Hockemeyer_AAVS1_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
A12
001-163803301R
CACCATGGCACATGTCTACTTGAG
Hockemeyer_AAVS1_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
B11
005-041303604F
GTCTTGAGAGAATGAGAAGCCAGG
Hockemeyer_AAVS1_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
B12
005-041303604R
CTCCTGGTCCCAAGTGATCC
Hockemeyer_AAVS1_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
C11
002-103259635F
GTTCTCTGCGTTCTTTACGTCGTC
Hockemeyer_AAVS1_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
C12
002-103259635R
GTCAACCTCACAAGGCACAGAAG
Hockemeyer_AAVS1_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
D11
012-071464141F
CCACAAGTTTGGGGAGCAAGAC
Hockemeyer_AAVS1_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
D12
012-071464141R
AAGCAAATGCCTAGTGTGGGAAGC
Hockemeyer_AAVS1_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
E11
001-202164461F
TGCTCAGTGTTGGGAGTGTCCT
Hockemeyer_AAVS1_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
E12
001-202164461R
TTTGCCAGATGCTGAGCGCC
Hockemeyer_AAVS1_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
F11
00X-040907277F
CTGGTTAGGAGGGTTGAAGGTG
Hockemeyer_AAVS1_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
F12
00X-040907277R
GGTCAGAGAAGGCTGAGTAGG
Hockemeyer_AAVS1_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
G11
003-109619335F
CAGGGAAGTCTCAGTGGTTTTTGG
Hockemeyer_AAVS1_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
G12
003-109619335R
CCAGGTGTATTCTGTGTGGCTAG
Hockemeyer_AAVS1_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
H11
003-015883800F
GCCAGGCACAAAAAGACAAAGTTTGCATG
Hockemeyer_AAVS1_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
H12
003-015883800R
GGGGTATCCATCACCTCAAGC
Hockemeyer_AAVS1_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
Primer Plate #3
A1
007-140379038F
TCTGCGCCCCTTTGTCACCA
Hockemeyer_AAVS1_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
A2
007-140379038R
CCTCTAAGGCAGGTCACCAAC
Hockemeyer_AAVS1_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
B1
016-081100645F
GCCCCCAAAGTGCATGTTTGGA
Hockemeyer_AAVS1_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
B2
016-081100645R
GAGCTCTCCGATAACATCATGGC
Hockemeyer_AAVS1_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
C1
007-140355184F
CCCACAAGGGCCTTATTTTCAGTC
Hockemeyer_AAVS1_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
C2
007-140355184R
GAGAGCTGATTTCACCCCTCTAG
Hockemeyer_AAVS1_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
D1
012-011342495F
CTGTCTTCCAGTCTAAAGCAACTACCTC
Hockemeyer_AAVS1_Ranking-TALENv2.0	Primer pair generated using optimal parameters. 
D2
012-011342495R
CCACACATCCTCTTCCTCCTATTC
Hockemeyer_AAVS1_Ranking-TALENv2.0	Primer pair generated using optimal parameters.