PROGNOS Summary for Hockemeyer_AAVS1_Ranking-RVDs_5TC

The hg19 genome was searched for the TALEN pair targeting:
5'- TTTTCTGTCACCAATCCT NN..NN ACTGTGGGGTGGAGGGGA -3'

Using these RVDs:
Left:  01NG02NG03NG04HD05NG06NN07NG08HD09NI10HD11HD12NI13NI14NG15HD16HD17NG
Right: 01HD02HD03HD04HD05NG06HD07HD08NI09HD10HD11HD12HD13NI14HD15NI16NN17NG

Up to 5 mismatches were allowed in each nuclease half-site.

Allowed spacing distances were: 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26

Only heterodimers of the nuclease pair were included in the output

207 total sites were located
Mismatch Types
Genomic Regions
  0 and 0: 1
  2 and 5: 1
  3 and 5: 4
  4 and 4: 1
  4 and 5: 45
  5 and 5: 155
  Exons: 8
  Promoters: 3
  Introns: 95
  Intergenic: 101


The top 100 sites are ranked below according to a weighting of the RVD base preferences. All sites listed contain flanking 5' pyrimidines.
For the full output, click here.

This output will be deleted from the server one week after it is generated.  Please save your results.

Primer pairs were designed using the 400 bp of sequence on either side of the cleavage site to melt close to 60 °C

For easy visualization of cleavage on gels:
The smallest fragment will be longer than 130 bp, which is also the minimum separation distance between uncleaved and cleaved products.
The two cleavage products will be separated by a minimum size of 0 bp
To optimize assay conditions, the amplicons have a minimum length of 260 bp, a maximum length of 450 bp, and an optimal length of 270 bp.
Rank: 1	Homology Score: 72	RVD Match Score: 2	Left Half-Site RVD Score: 2.343	Right Half-Site RVD Score: 2.083
	Genomic Region: Intron	Closest Gene: PPP1R12C	Caution! This site scores very closely to the intended nuclease target. The potential for cleavage at this site is of great concern.
Match Type: L15R
	           Left Nuclease
	         TTTTCTGTCACCAATCCT
	5'-GGGGC TTTTCTGTCACCAATCCT      GTCCCTAGTGGCCCC       ACTGTGGGGTGGAGGGGA CAGAT-3'
	3'-CCCCG AAAAGACAGTGGTTAGGA      CAGGGATCACCGGGG       TGACACCCCACCTCCCCT GTCTA-5'
	                                                       TGACACCCCACCTCCCCT
	                                                            Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 019-055627003
Forward Primer: 5'-CCAGGAAATGGGGGTGTGTC-3' Tm: 59.508
Reverse Primer: 5'-GTCCACTTCAGGACAGCATGTTTG-3' Tm: 59.038
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 131, 139
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr19:55627107-55627157

Rank: 2	Homology Score: 5	RVD Match Score: 3.145	Left Half-Site RVD Score: 5.991	Right Half-Site RVD Score: 4.981
	Genomic Region: Intron	Closest Gene: TEX264
Match Type: L24R
	           Left Nuclease
	         TTTTCTGTCACCAATCCT
	5'-TTCCC TTTTTTGCCAACACTCCT  GAGTGAGTAAGCAGGTGTTGGTGC  AGGGTGGGGTGGTGGTGG GGAAG-3'
	3'-AAGGG AAAAAACGGTTGTGAGGA  CTCACTCATTCGTCCACAACCACG  TCCCACCCCACCACCACC CCTTC-5'
	                                                       TGACACCCCACCTCCCCT
	                                                            Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 003-051729704
Forward Primer: 5'-GGAGATGAACTCAGGCTGAAGTG-3' Tm: 59.148
Reverse Primer: 5'-TTCCCTCCCACCTGGGTCTT-3' Tm: 59.508
Expected PCR Product Length: 271
Expected CelI Cleavage Product Sizes: 141, 130
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr3:51729813-51729872

Rank: 3	Homology Score: 17	RVD Match Score: 3.15	Left Half-Site RVD Score: 3.963	Right Half-Site RVD Score: 7.356
	Genomic Region: Intergenic	Closest Gene: TFEC
Match Type: R11L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-CCCCC TCCCCGCCACCCCCCAGT        AATGGGGAGAG         AGGACATTTGACAGAGAA TGAAG-3'
	3'-GGGGG AGGGGCGGTGGGGGGTCA        TTACCCCTCTC         TCCTGTAAACTGTCTCTT ACTTC-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 007-115754830
Forward Primer: 5'-CGGTTGAGACTGAAGCATGCAAAG-3' Tm: 59.038
Reverse Primer: 5'-AAGGACCACACTACTGACATTAGATCTACC-3' Tm: 60.058
Expected PCR Product Length: 274
Expected CelI Cleavage Product Sizes: 143, 131
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr7:115754948-115754994

Rank: 4	Homology Score: 10	RVD Match Score: 3.161	Left Half-Site RVD Score: 3.845	Right Half-Site RVD Score: 7.621
	Genomic Region: Intron	Closest Gene: STPG1
Match Type: R11L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-ACACT TCCCCACCACACCACAAT        GCCCTGTCTCA         AGAAACTGTGACAGAATA AAATG-3'
	3'-TGTGA AGGGGTGGTGTGGTGTTA        CGGGACAGAGT         TCTTTGACACTGTCTTAT TTTAC-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 001-024697363
Forward Primer: 5'-AGCCTCCCAAAGTGCTAGGAC-3' Tm: 59.378
Reverse Primer: 5'-GTGGAGAACATCCATTCACAGGG-3' Tm: 59.148
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 135, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr1:24697473-24697519

Rank: 5	Homology Score: 5	RVD Match Score: 3.179	Left Half-Site RVD Score: 5.326	Right Half-Site RVD Score: 5.853
	Genomic Region: Intergenic	Closest Gene: AMIGO2
Match Type: R11L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-GGATC CTCCCACCACCCCACGAT        GTAAAACTTGA         AGGGTTGATGGCTGAAAA AATAT-3'
	3'-CCTAG GAGGGTGGTGGGGTGCTA        CATTTTGAACT         TCCCAACTACCGACTTTT TTATA-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 012-047346370
Forward Primer: 5'-CTTGGTATGTTCCAGTGTGGCC-3' Tm: 59.258
Reverse Primer: 5'-GGATGTGTGCAGGATGTACAGG-3' Tm: 59.258
Expected PCR Product Length: 272
Expected CelI Cleavage Product Sizes: 134, 138
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr12:47346479-47346525

Rank: 6	Homology Score: 5	RVD Match Score: 3.195	Left Half-Site RVD Score: 6.316	Right Half-Site RVD Score: 5.03
	Genomic Region: Intron	Closest Gene: DMD
Match Type: L23R
	           Left Nuclease
	         TTTTCTGTCACCAATCCT
	5'-TCTTC TCTTCCATCACTAATTCT  GATGAACAGCTATCAAGCTATTC   ATGGTAGGGTGGAGGGTA GGTGG-3'
	3'-AGAAG AGAAGGTAGTGATTAAGA  CTACTTGTCGATAGTTCGATAAG   TACCATCCCACCTCCCAT CCACC-5'
	                                                       TGACACCCCACCTCCCCT
	                                                            Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 00X-031479613
Forward Primer: 5'-GTCTGGACATGTCTGAATGCCTAG-3' Tm: 59.038
Reverse Primer: 5'-GGTTAGGTAAAGTGTCCCCTGC-3' Tm: 59.258
Expected PCR Product Length: 339
Expected CelI Cleavage Product Sizes: 156, 183
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chrX:31479738-31479796

Rank: 7	Homology Score: 2	RVD Match Score: 3.224	Left Half-Site RVD Score: 6.495	Right Half-Site RVD Score: 5.07
	Genomic Region: Intron	Closest Gene: TRPM3
Match Type: L16R
	           Left Nuclease
	         TTTTCTGTCACCAATCCT
	5'-TCCTC CTTTCTGTCCAGAATCGT      TGGTTAGTTTTTGAAG      GTTGTGCGGTGGAGGTGG TTGGG-3'
	3'-AGGAG GAAAGACAGGTCTTAGCA      ACCAATCAAAAACTTC      CAACACGCCACCTCCACC AACCC-5'
	                                                       TGACACCCCACCTCCCCT
	                                                            Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 009-073729326
Forward Primer: 5'-CCACATGCTCAAGAGCCAGG-3' Tm: 59.508
Reverse Primer: 5'-GGCATGTCCCTTAGATTCCAAGGT-3' Tm: 59.038
Expected PCR Product Length: 330
Expected CelI Cleavage Product Sizes: 186, 144
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr9:73729484-73729535

Rank: 8	Homology Score: 2	RVD Match Score: 3.226	Left Half-Site RVD Score: 5.002	Right Half-Site RVD Score: 6.591
	Genomic Region: Intergenic	Closest Gene: RTN4RL2
Match Type: R13L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-TCGTC CCCCCTCCCCCCAACCCT       AACCGGAGCTAGG        TGGGGTGGGGACAGCAAA TGTGG-3'
	3'-AGCAG GGGGGAGGGGGGTTGGGA       TTGGCCTCGATCC        ACCCCACCCCTGTCGTTT ACACC-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 011-057225300
Forward Primer: 5'-AGCCTCACCGTACAGATGAGGA-3' Tm: 59.258
Reverse Primer: 5'-TTTAGGTGGCTGGCTGGTGG-3' Tm: 59.508
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 130, 140
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr11:57225404-57225452

Rank: 9	Homology Score: 2	RVD Match Score: 3.236	Left Half-Site RVD Score: 5.304	Right Half-Site RVD Score: 6.309
	Genomic Region: Intron	Closest Gene: LGR6
Match Type: R22L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-GATGT CCCCCTCCACTCCAAAAG   GCACAGGCTGTGGGCCAGGTAA   GGGAGTGGGAAGAGAAAA ATAAT-3'
	3'-CTACA GGGGGAGGTGAGGTTTTC   CGTGTCCGACACCCGGTCCATT   CCCTCACCCTTCTCTTTT TATTA-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 001-202164461
Forward Primer: 5'-TGCTCAGTGTTGGGAGTGTCCT-3' Tm: 59.258
Reverse Primer: 5'-TTTGCCAGATGCTGAGCGCC-3' Tm: 59.508
Expected PCR Product Length: 276
Expected CelI Cleavage Product Sizes: 131, 145
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr1:202164561-202164618

Rank: 10	Homology Score: 5	RVD Match Score: 3.245	Left Half-Site RVD Score: 5.045	Right Half-Site RVD Score: 6.688
	Genomic Region: Intergenic	Closest Gene: FBN2
Match Type: R20L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-GACTT CCACCTCCACCTAACAGT    AATGAGATGCCCCTGTCTGC    TGGGATGGTGTCAGAAGA TACCT-3'
	3'-CTGAA GGTGGAGGTGGATTGTCA    TTACTCTACGGGGACAGACG    ACCCTACCACAGTCTTCT ATGGA-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 005-127879056
Forward Primer: 5'-CTCCTGGATAGTGTCAGAGAAAGC-3' Tm: 59.038
Reverse Primer: 5'-CTGCTCCCAATGTAGTTTCCCATG-3' Tm: 59.038
Expected PCR Product Length: 267
Expected CelI Cleavage Product Sizes: 136, 131
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr5:127879162-127879217

Rank: 11	Homology Score: 5	RVD Match Score: 3.25	Left Half-Site RVD Score: 6.391	Right Half-Site RVD Score: 5.326
	Genomic Region: Intergenic	Closest Gene: MRM1
Match Type: L25R
	           Left Nuclease
	         TTTTCTGTCACCAATCCT
	5'-TTCTT TTATCTGCCCCCAACACT CCAGGCATTATGTGCATTCATAAAA  AAGGGGGGGTGGAGTGGA GAGAA-3'
	3'-AAGAA AATAGACGGGGGTTGTGA GGTCCGTAATACACGTAAGTATTTT  TTCCCCCCCACCTCACCT CTCTT-5'
	                                                       TGACACCCCACCTCCCCT
	                                                            Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 017-035084330
Forward Primer: 5'-GAGCCTCTGCTGGTTGGATG-3' Tm: 59.508
Reverse Primer: 5'-ATGTTGGGTGCTGGGGAAGG-3' Tm: 59.508
Expected PCR Product Length: 287
Expected CelI Cleavage Product Sizes: 131, 156
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr17:35084429-35084489

Rank: 12	Homology Score: 5	RVD Match Score: 3.264	Left Half-Site RVD Score: 5.57	Right Half-Site RVD Score: 6.222
	Genomic Region: Intergenic	Closest Gene: ABRA
Match Type: R12L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-CTGTT TCCCCACCCTCCCACATT        ATGTACCCTGGG        AGGAAGGGTGCCAGAGAG AGGCT-3'
	3'-GACAA AGGGGTGGGAGGGTGTAA        TACATGGGACCC        TCCTTCCCACGGTCTCTC TCCGA-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 008-107822970
Forward Primer: 5'-GGCTAAGTGTGAGAATCGAGTGAG-3' Tm: 59.038
Reverse Primer: 5'-GTTAGTGAGACAGCATGTCCCTTC-3' Tm: 59.038
Expected PCR Product Length: 280
Expected CelI Cleavage Product Sizes: 150, 130
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr8:107823094-107823141

Rank: 13	Homology Score: 2	RVD Match Score: 3.268	Left Half-Site RVD Score: 5.466	Right Half-Site RVD Score: 6.369
	Genomic Region: Intron	Closest Gene: SMOC2
Match Type: R25L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-CCTCC CCCCCTCCCCCACAAAGA TTTGAAGCCTAACATTGCTGGGGGC  AGGATTAGAGGTTGAAAA CAAAG-3'
	3'-GGAGG GGGGGAGGGGGTGTTTCT AAACTTCGGATTGTAACGACCCCCG  TCCTAATCTCCAACTTTT GTTTC-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 006-168932997
Forward Primer: 5'-TCCTTCGTCCTGGGATTGGG-3' Tm: 59.508
Reverse Primer: 5'-GGGTGCACAGTTACAGAGGAG-3' Tm: 59.378
Expected PCR Product Length: 280
Expected CelI Cleavage Product Sizes: 149, 131
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr6:168933114-168933174

Rank: 14	Homology Score: 5	RVD Match Score: 3.274	Left Half-Site RVD Score: 7.511	Right Half-Site RVD Score: 4.588
	Genomic Region: Intron	Closest Gene: LRRC4C
Match Type: L14R
	           Left Nuclease
	         TTTTCTGTCACCAATCCT
	5'-TAAAA TTGTCTGTCACACATTGT       TCTGTTAATTGCCA       ACTGGGGGGGGTAGGGGG AGGAA-3'
	3'-ATTTT AACAGACAGTGTGTAACA       AGACAATTAACGGT       TGACCCCCCCCATCCCCC TCCTT-5'
	                                                       TGACACCCCACCTCCCCT
	                                                            Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 011-040808649
Forward Primer: 5'-CCCATGTCTCAGTCTCTGTTCTAC-3' Tm: 59.038
Reverse Primer: 5'-CTCACTTGTCAGCAAGCACAAAGG-3' Tm: 59.038
Expected PCR Product Length: 309
Expected CelI Cleavage Product Sizes: 155, 154
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr11:40808777-40808826

Rank: 15	Homology Score: 2	RVD Match Score: 3.288	Left Half-Site RVD Score: 4.892	Right Half-Site RVD Score: 7.228
	Genomic Region: Intron	Closest Gene: PKHD1L1
Match Type: R15L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-CCCCC CTCCCCCCACCCCACAAC      AGGCCCCAGGGACAC       AGGAAGGGGAACATAAAA CATAG-3'
	3'-GGGGG GAGGGGGGTGGGGTGTTG      TCCGGGGTCCCTGTG       TCCTTCCCCTTGTATTTT GTATC-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 008-110449611
Forward Primer: 5'-GTGCACAACGTGCAGGTTAGTTAC-3' Tm: 59.038
Reverse Primer: 5'-CCTGCAGATTGCAAATACATAATACCCCC-3' Tm: 60.178
Expected PCR Product Length: 293
Expected CelI Cleavage Product Sizes: 138, 155
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr8:110449722-110449772

Rank: 16	Homology Score: 5	RVD Match Score: 3.29	Left Half-Site RVD Score: 6.091	Right Half-Site RVD Score: 5.853
	Genomic Region: Exon	Closest Gene: CATSPERG
Match Type: R24L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-TCAAT CCCCTCCAACCCCATAGT  TTTCTACCCCTTCTTCTTGATTCA  AGATTTGGTGACAGGAGA CTCCG-3'
	3'-AGTTA GGGGAGGTTGGGGTATCA  AAAGATGGGGAAGAAGAACTAAGT  TCTAAACCACTGTCCTCT GAGGC-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 019-038858026
Forward Primer: 5'-TTCCTCTCCCCGCAGACTAC-3' Tm: 59.508
Reverse Primer: 5'-AGTGCACACAGACTGGCCTC-3' Tm: 59.508
Expected PCR Product Length: 281
Expected CelI Cleavage Product Sizes: 138, 143
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr19:38858132-38858191

Rank: 17	Homology Score: 10	RVD Match Score: 3.291	Left Half-Site RVD Score: 7.204	Right Half-Site RVD Score: 4.928
	Genomic Region: Intron	Closest Gene: ERCC5
Match Type: L20R
	           Left Nuclease
	         TTTTCTGTCACCAATCCT
	5'-CTTTA TTTTCTTTCTGCAACCAT    GAAGTCTCTGGAAGTGGTGG    ACTGTAGGGTGGTGTGGA GTAGC-3'
	3'-GAAAT AAAAGAAAGACGTTGGTA    CTTCAGAGACCTTCACCACC    TGACATCCCACCACACCT CATCG-5'
	                                                       TGACACCCCACCTCCCCT
	                                                            Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 013-103511881
Forward Primer: 5'-TCTCTGGCTGGCAGTTGAGG-3' Tm: 59.508
Reverse Primer: 5'-CAGGTCACTGAAAACTGCAAACCG-3' Tm: 59.038
Expected PCR Product Length: 346
Expected CelI Cleavage Product Sizes: 131, 215
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr13:103511982-103512037

Rank: 18	Homology Score: 5	RVD Match Score: 3.295	Left Half-Site RVD Score: 6.076	Right Half-Site RVD Score: 5.904
	Genomic Region: Intron	Closest Gene: HEG1
Match Type: R18L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-CTCTA TCCCTTCCACCTCACCAA     ATAGTCCTCGCTAGCAGA     AGGGTTGGGTACAGAACA ACAGT-3'
	3'-GAGAT AGGGAAGGTGGAGTGGTT     TATCAGGAGCGATCGTCT     TCCCAACCCATGTCTTGT TGTCA-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 003-124702567
Forward Primer: 5'-TGCAAGTCTACAACCAGGGTGTG-3' Tm: 59.148
Reverse Primer: 5'-CAGAAGGCAGCTGGAAGTCTC-3' Tm: 59.378
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 134, 136
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr3:124702672-124702725

Rank: 19	Homology Score: 5	RVD Match Score: 3.296	Left Half-Site RVD Score: 6.1	Right Half-Site RVD Score: 5.891
	Genomic Region: Intron	Closest Gene: TMEM9
Match Type: R20L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-GCAGC CACCCTCTTCCCCACATT    CCTTCTCCAACTTCTTAGGT    AGGATTGGGGAAACATAA AGGGC-3'
	3'-CGTCG GTGGGAGAAGGGGTGTAA    GGAAGAGGTTGAAGAATCCA    TCCTAACCCCTTTGTATT TCCCG-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 001-201121271
Forward Primer: 5'-GAGGGATGAGGGGAAATGGG-3' Tm: 59.508
Reverse Primer: 5'-GGAGCTGTTTGGGTAGATCTATGG-3' Tm: 59.038
Expected PCR Product Length: 373
Expected CelI Cleavage Product Sizes: 131, 242
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr1:201121372-201121427

Rank: 20	Homology Score: 2	RVD Match Score: 3.303	Left Half-Site RVD Score: 5.935	Right Half-Site RVD Score: 6.107
	Genomic Region: Intron	Closest Gene: KIAA0556
Match Type: L21R
	           Left Nuclease
	         TTTTCTGTCACCAATCCT
	5'-GGACC CATTCAGTGACCACTCCT   ATAGTTCCCTAGTTCACTTGT    CTTGTGGGGGTGAGAGGA GGGAC-3'
	3'-CCTGG GTAAGTCACTGGTGAGGA   TATCAAGGGATCAAGTGAACA    GAACACCCCCACTCTCCT CCCTG-5'
	                                                       TGACACCCCACCTCCCCT
	                                                            Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 016-027592598
Forward Primer: 5'-CCCTGTGCACATAGGAATAGCC-3' Tm: 59.258
Reverse Primer: 5'-GGGTGATGGGTGCATCAATATCTC-3' Tm: 59.038
Expected PCR Product Length: 416
Expected CelI Cleavage Product Sizes: 241, 175
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr16:27592809-27592865

Rank: 21	Homology Score: 2	RVD Match Score: 3.307	Left Half-Site RVD Score: 6.769	Right Half-Site RVD Score: 5.388
	Genomic Region: Intron	Closest Gene: SLC24A3
Match Type: L18R
	           Left Nuclease
	         TTTTCTGTCACCAATCCT
	5'-CCATT CTTGGTGTCAGAAATCCT     TGGGCTCTCTAAGCCCCA     GTTGTGGGGAGGAGGGCG GTAGG-3'
	3'-GGTAA GAACCACAGTCTTTAGGA     ACCCGAGAGATTCGGGGT     CAACACCCCTCCTCCCGC CATCC-5'
	                                                       TGACACCCCACCTCCCCT
	                                                            Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 020-019256133
Forward Primer: 5'-GGAAGGGTTTGCAAGCCCTG-3' Tm: 59.508
Reverse Primer: 5'-AAGCAAGCCGCATGCACTAAGG-3' Tm: 59.258
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 135, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr20:19256239-19256292

Rank: 22	Homology Score: 5	RVD Match Score: 3.316	Left Half-Site RVD Score: 6.275	Right Half-Site RVD Score: 5.88
	Genomic Region: Intergenic	Closest Gene: LOC729013
Match Type: L25R
	           Left Nuclease
	         TTTTCTGTCACCAATCCT
	5'-AGGTC TCCTCTGACACCACTCCA GTGAGTAGTGGAAAGATGCCTTATT  ACTGCTGGGTGGGGGTGA AGGTC-3'
	3'-TCCAG AGGAGACTGTGGTGAGGT CACTCATCACCTTTCTACGGAATAA  TGACGACCCACCCCCACT TCCAG-5'
	                                                       TGACACCCCACCTCCCCT
	                                                            Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 011-011075984
Forward Primer: 5'-GCCTCATTACTGCTAGGTGGAAG-3' Tm: 59.148
Reverse Primer: 5'-CACCCTAACATCCAGGCATGATGA-3' Tm: 59.038
Expected PCR Product Length: 316
Expected CelI Cleavage Product Sizes: 152, 164
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr11:11076104-11076164

Rank: 23	Homology Score: 5	RVD Match Score: 3.319	Left Half-Site RVD Score: 5.305	Right Half-Site RVD Score: 6.964
	Genomic Region: Intron	Closest Gene: LOC730102
Match Type: R25L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-AACCA TCCCCTCCAACCCCCATC AGTCCATGGAAAAACTGTCTTCCAC  AGAACTGGTGCCAAAAAG GTTGC-3'
	3'-TTGGT AGGGGAGGTTGGGGGTAG TCAGGTACCTTTTTGACAGAAGGTG  TCTTGACCACGGTTTTTC CAACG-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 001-178005473
Forward Primer: 5'-CTCACAGAAGTGCGAACCGTATTG-3' Tm: 59.038
Reverse Primer: 5'-CACCAATATGGCCACCTCTGTAC-3' Tm: 59.148
Expected PCR Product Length: 315
Expected CelI Cleavage Product Sizes: 148, 167
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr1:178005589-178005649

Rank: 24	Homology Score: 2	RVD Match Score: 3.32	Left Half-Site RVD Score: 6.486	Right Half-Site RVD Score: 5.719
	Genomic Region: Intergenic	Closest Gene: AXIN2
Match Type: L20R
	           Left Nuclease
	         TTTTCTGTCACCAATCCT
	5'-AAAGT TTTTCCATCAACATTTCT    AAATGGAAGAGAGAAGGCAA    AAAGTGGGGTGGGGGTGG GAGGG-3'
	3'-TTTCA AAAAGGTAGTTGTAAAGA    TTTACCTTCTCTCTTCCGTT    TTTCACCCCACCCCCACC CTCCC-5'
	                                                       TGACACCCCACCTCCCCT
	                                                            Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 017-063492561
Forward Primer: 5'-CTCTGTTCCCCACTGATCACC-3' Tm: 59.378
Reverse Primer: 5'-AGCATGGAAGGTCCCCCACA-3' Tm: 59.508
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 135, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr17:63492666-63492721

Rank: 25	Homology Score: 5	RVD Match Score: 3.323	Left Half-Site RVD Score: 6.587	Right Half-Site RVD Score: 5.595
	Genomic Region: Intron	Closest Gene: PVRL1
Match Type: R17L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-TAAAG TCCTCTGCCACCCACACT     CTTCATGAGATCCCAAA      AGGATTGATCATGGAAAA TGCAA-3'
	3'-ATTTC AGGAGACGGTGGGTGTGA     GAAGTACTCTAGGGTTT      TCCTAACTAGTACCTTTT ACGTT-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 011-119574363
Forward Primer: 5'-CAACGAGGCTGACGCTCTTTC-3' Tm: 59.378
Reverse Primer: 5'-CTGAGCACCTGTGTGCCAGT-3' Tm: 59.508
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 135, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr11:119574470-119574522

Rank: 26	Homology Score: 2	RVD Match Score: 3.328	Left Half-Site RVD Score: 6.112	Right Half-Site RVD Score: 6.119
	Genomic Region: Intron	Closest Gene: AUTS2
Match Type: R12L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-ACCCA CCCCTTCCCTCACACAGT        CTCAGGCAATTA        TTGATTTGTGAGGGAAAA GGGGT-3'
	3'-TGGGT GGGGAAGGGAGTGTGTCA        GAGTCCGTTAAT        AACTAAACACTCCCTTTT CCCCA-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 007-069107597
Forward Primer: 5'-GATGTCAGTGTCCTCAGCTGTTAG-3' Tm: 59.038
Reverse Primer: 5'-CCTGACTCACAGAAACTGTGGG-3' Tm: 59.258
Expected PCR Product Length: 319
Expected CelI Cleavage Product Sizes: 156, 163
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr7:69107727-69107774

Rank: 27	Homology Score: 2	RVD Match Score: 3.331	Left Half-Site RVD Score: 6.028	Right Half-Site RVD Score: 6.229
	Genomic Region: Intergenic	Closest Gene: ADTRP
Match Type: R14L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-TAGCT TCCCTCCCACCCCCCACC       CTGGAACAACAGAT       TGGGTTAGTGCTAGAAAA TGTCT-3'
	3'-ATCGA AGGGAGGGTGGGGGGTGG       GACCTTGTTGTCTA       ACCCAATCACGATCTTTT ACAGA-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Warning! Primer pair was generated using suboptimal parameters. 
Primer Index: 006-011853744
Forward Primer: 5'-GTCTACGGATCTAACACTCCAAAAATCTTAGC-3' Tm: 59.848
Reverse Primer: 5'-CTAGGGGGCTGAAATGGAACAAC-3' Tm: 59.148
Expected PCR Product Length: 325
Expected CelI Cleavage Product Sizes: 172, 153
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr6:11853889-11853938

Rank: 28	Homology Score: 2	RVD Match Score: 3.332	Left Half-Site RVD Score: 6.732	Right Half-Site RVD Score: 5.591
	Genomic Region: Promoter	Closest Gene: RASSF5
Match Type: L25R
	           Left Nuclease
	         TTTTCTGTCACCAATCCT
	5'-TTTTT TCTTCTCTAACCATTCCG ATTGACCCGGCTCCCCCACCCAGCG  AATGGGGGAGGGAGGGGG TGGGG-3'
	3'-AAAAA AGAAGAGATTGGTAAGGC TAACTGGGCCGAGGGGGTGGGTCGC  TTACCCCCTCCCTCCCCC ACCCC-5'
	                                                       TGACACCCCACCTCCCCT
	                                                            Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 001-206729981
Forward Primer: 5'-CTCTCTAGGTTGCCATAACTCTGC-3' Tm: 59.038
Reverse Primer: 5'-TCTCATCAGCCCCGGTGACT-3' Tm: 59.508
Expected PCR Product Length: 287
Expected CelI Cleavage Product Sizes: 155, 132
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr1:206730104-206730164

Rank: 29	Homology Score: 5	RVD Match Score: 3.335	Left Half-Site RVD Score: 4.892	Right Half-Site RVD Score: 7.621
	Genomic Region: Intron	Closest Gene: ARHGAP10
Match Type: R17L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-TCCTG TTCCCACCACCCCACAAC     CTTCTTACATCTGAACA      ATGACAGATGACAGTAAA TCCAG-3'
	3'-AGGAC AAGGGTGGTGGGGTGTTG     GAAGAATGTAGACTTGT      TACTGTCTACTGTCATTT AGGTC-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 004-148942680
Forward Primer: 5'-GTAGGTAAGCCAGGGAGCTGT-3' Tm: 59.378
Reverse Primer: 5'-CCTGCCAACACCATGTTTCCAC-3' Tm: 59.258
Expected PCR Product Length: 299
Expected CelI Cleavage Product Sizes: 158, 141
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr4:148942810-148942862

Rank: 30	Homology Score: 2	RVD Match Score: 3.345	Left Half-Site RVD Score: 6.409	Right Half-Site RVD Score: 5.94
	Genomic Region: Intergenic	Closest Gene: BUD13
Match Type: R23L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-CACCA TCTCCCTCCCCCCACACT  CCCATACCCCAATCCTCCTAGGC   AGAATGGGAGAGAGAAAG AAAGA-3'
	3'-GTGGT AGAGGGAGGGGGGTGTGA  GGGTATGGGGTTAGGAGGATCCG   TCTTACCCTCTCTCTTTC TTTCT-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 011-116565112
Forward Primer: 5'-CATCTATGCCCCACCAAGAGG-3' Tm: 59.378
Reverse Primer: 5'-ACATCTGTGGCAGGTGGAGC-3' Tm: 59.508
Expected PCR Product Length: 279
Expected CelI Cleavage Product Sizes: 137, 142
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr11:116565218-116565276

Rank: 31	Homology Score: 2	RVD Match Score: 3.349	Left Half-Site RVD Score: 6.288	Right Half-Site RVD Score: 6.1
	Genomic Region: Exon	Closest Gene: CBLN2
Match Type: L13R
	           Left Nuclease
	         TTTTCTGTCACCAATCCT
	5'-CTATA TTTTCTCTCCTCAACCCC       AGCAAGAAAAATA        AAAGTGGGGTTGAGGAGG GAGAG-3'
	3'-GATAT AAAAGAGAGGAGTTGGGG       TCGTTCTTTTTAT        TTTCACCCCAACTCCTCC CTCTC-5'
	                                                       TGACACCCCACCTCCCCT
	                                                            Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 018-070204440
Forward Primer: 5'-CCACTCAGGAACACCAGGTTCT-3' Tm: 59.258
Reverse Primer: 5'-CATTGGGAACCTGTCAAGAAGTGC-3' Tm: 59.038
Expected PCR Product Length: 419
Expected CelI Cleavage Product Sizes: 267, 152
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr18:70204681-70204729

Rank: 32	Homology Score: 5	RVD Match Score: 3.351	Left Half-Site RVD Score: 6.125	Right Half-Site RVD Score: 6.27
	Genomic Region: Intergenic	Closest Gene: TMTC1
Match Type: L12R
	           Left Nuclease
	         TTTTCTGTCACCAATCCT
	5'-GATTA TTTTCTAACAACCTTCCT        ACTGAGGAACAT        ACTCTGGGATGGATTGGA AGTGC-3'
	3'-CTAAT AAAAGATTGTTGGAAGGA        TGACTCCTTGTA        TGAGACCCTACCTAACCT TCACG-5'
	                                                       TGACACCCCACCTCCCCT
	                                                            Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 012-030180063
Forward Primer: 5'-CTATAAGGCTGCTGGGCCTTTC-3' Tm: 59.258
Reverse Primer: 5'-GAGGCAAACCTACTTTATGGAGGG-3' Tm: 59.038
Expected PCR Product Length: 333
Expected CelI Cleavage Product Sizes: 131, 202
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr12:30180168-30180215

Rank: 33	Homology Score: 5	RVD Match Score: 3.352	Left Half-Site RVD Score: 5.506	Right Half-Site RVD Score: 6.897
	Genomic Region: Intron	Closest Gene: BOP1
Match Type: L22R
	           Left Nuclease
	         TTTTCTGTCACCAATCCT
	5'-CCAAA TTTCCTCTCCCTAATCCT   GCAGGAGCCCACAGCTGGCCAG   AGGGAGGGGAGGAGAGGA CAAGC-3'
	3'-GGTTT AAAGGAGAGGGATTAGGA   CGTCCTCGGGTGTCGACCGGTC   TCCCTCCCCTCCTCTCCT GTTCG-5'
	                                                       TGACACCCCACCTCCCCT
	                                                            Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 008-145493530
Forward Primer: 5'-GAGCCATTAAGGGCTAGAGGG-3' Tm: 59.378
Reverse Primer: 5'-GGAAAGCTCCCAAGAAGGGAAG-3' Tm: 59.258
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 135, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr8:145493634-145493691

Rank: 34	Homology Score: 5	RVD Match Score: 3.352	Left Half-Site RVD Score: 5.506	Right Half-Site RVD Score: 6.897
	Genomic Region: Intergenic	Closest Gene: SCXB
Match Type: L22R
	           Left Nuclease
	         TTTTCTGTCACCAATCCT
	5'-CCAAA TTTCCTCTCCCTAATCCT   GCAGGAGCCCACAGCTGGCCAG   AGGGAGGGGAGGAGAGGA CAAGC-3'
	3'-GGTTT AAAGGAGAGGGATTAGGA   CGTCCTCGGGTGTCGACCGGTC   TCCCTCCCCTCCTCTCCT GTTCG-5'
	                                                       TGACACCCCACCTCCCCT
	                                                            Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 008-145324445
Forward Primer: 5'-GAGCCATTAAGGGCTAGAGGG-3' Tm: 59.378
Reverse Primer: 5'-GGAAAGCTCCCAAGAAGGGAAG-3' Tm: 59.258
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 135, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr8:145324549-145324606

Rank: 35	Homology Score: 5	RVD Match Score: 3.363	Left Half-Site RVD Score: 6.352	Right Half-Site RVD Score: 6.144
	Genomic Region: Intergenic	Closest Gene: SYT6
Match Type: L18R
	           Left Nuclease
	         TTTTCTGTCACCAATCCT
	5'-TTTCC CTTTCTGATAATAATCCT     ATAATGAACTCTAGGCAC     TCTGCTGGGTGGAGAGGA TATAA-3'
	3'-AAAGG GAAAGACTATTATTAGGA     TATTACTTGAGATCCGTG     AGACGACCCACCTCTCCT ATATT-5'
	                                                       TGACACCCCACCTCCCCT
	                                                            Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 001-114585589
Forward Primer: 5'-CCCCATAAATGATCTGCATCCAGG-3' Tm: 59.038
Reverse Primer: 5'-CGCTGAGAAGGCCAGATGAG-3' Tm: 59.508
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 135, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr1:114585695-114585748

Rank: 36	Homology Score: 5	RVD Match Score: 3.364	Left Half-Site RVD Score: 5.827	Right Half-Site RVD Score: 6.708
	Genomic Region: Intergenic	Closest Gene: C20orf197
Match Type: R16L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-GCACC TCCCATCAACCCCATAGC      AGGAATGGGGCGAACT      TTGATTGATGTAAGAAAA TGAAA-3'
	3'-CGTGG AGGGTAGTTGGGGTATCG      TCCTTACCCCGCTTGA      AACTAACTACATTCTTTT ACTTT-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 020-058618528
Forward Primer: 5'-GGGAGCTATGATCATGCCATAGG-3' Tm: 59.148
Reverse Primer: 5'-GCTCCATGTCATTGTTCTGGGC-3' Tm: 59.258
Expected PCR Product Length: 290
Expected CelI Cleavage Product Sizes: 135, 155
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr20:58618635-58618686

Rank: 37	Homology Score: 8	RVD Match Score: 3.364	Left Half-Site RVD Score: 6.153	Right Half-Site RVD Score: 6.349
	Genomic Region: Intergenic	Closest Gene: INADL
Match Type: R25L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-TCAAC TCCTCTCAACCCCAGAGC CATGTGTTACTCTTTGGGGAACTTC  AGGATAGCTGTCTGAAAA CAGTC-3'
	3'-AGTTG AGGAGAGTTGGGGTCTCG GTACACAATGAGAAACCCCTTGAAG  TCCTATCGACAGACTTTT GTCAG-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 001-062633493
Forward Primer: 5'-TCCTCCTGCCTAATGGCTTTACTG-3' Tm: 59.038
Reverse Primer: 5'-GCTACTGAGCCCAGCCTAATTTAC-3' Tm: 59.038
Expected PCR Product Length: 273
Expected CelI Cleavage Product Sizes: 130, 143
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr1:62633591-62633651

Rank: 38	Homology Score: 2	RVD Match Score: 3.366	Left Half-Site RVD Score: 6.792	Right Half-Site RVD Score: 5.769
	Genomic Region: Intron	Closest Gene: COPE
Match Type: L21R
	           Left Nuclease
	         TTTTCTGTCACCAATCCT
	5'-ACACA CTTTCTGTGACCAGTGCC   CTGGACATGGCCCGGAGTCAC    ATCCTAGGGTGGAGGGGG GCACC-3'
	3'-TGTGT GAAAGACACTGGTCACGG   GACCTGTACCGGGCCTCAGTG    TAGGATCCCACCTCCCCC CGTGG-5'
	                                                       TGACACCCCACCTCCCCT
	                                                            Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 019-019017199
Forward Primer: 5'-GGGATGGGAGCAGATCCACA-3' Tm: 59.508
Reverse Primer: 5'-AAGCCTTCTGTGTCTCGGCC-3' Tm: 59.508
Expected PCR Product Length: 273
Expected CelI Cleavage Product Sizes: 130, 143
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr19:19017299-19017355

Rank: 39	Homology Score: 2	RVD Match Score: 3.368	Left Half-Site RVD Score: 7.808	Right Half-Site RVD Score: 4.96
	Genomic Region: Intron	Closest Gene: GHRHR
Match Type: L21R
	           Left Nuclease
	         TTTTCTGTCACCAATCCT
	5'-AAACA TTTGCTGACATGGATCCT   GCTAGACATTGAGGGTGTGTG    TGTGTGGGGTGGGGGTGG GGGCC-3'
	3'-TTTGT AAACGACTGTACCTAGGA   CGATCTGTAACTCCCACACAC    ACACACCCCACCCCCACC CCCGG-5'
	                                                       TGACACCCCACCTCCCCT
	                                                            Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 007-031005868
Forward Primer: 5'-GTCAAAGTCTCTACTCTCTGACCC-3' Tm: 59.038
Reverse Primer: 5'-GGGAGAAGAGCATCTGGAACAGAA-3' Tm: 59.038
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 131, 139
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr7:31005969-31006025

Rank: 40	Homology Score: 2	RVD Match Score: 3.368	Left Half-Site RVD Score: 6.586	Right Half-Site RVD Score: 5.927
	Genomic Region: Intergenic	Closest Gene: LGALSL
Match Type: R26L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-CTGCC TCCCCTCCCCACAATATT TACAAGCATTGATTTTCTAGAAGGGA AGGGGTGGGGAGGGAAAA AGGAG-3'
	3'-GACGG AGGGGAGGGGTGTTATAA ATGTTCGTAACTAAAAGATCTTCCCT TCCCCACCCCTCCCTTTT TCCTC-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 002-064716657
Forward Primer: 5'-GAAATGCATCCCCTCTAGCCTG-3' Tm: 59.258
Reverse Primer: 5'-GCTGCATGGACAGTGCTCACA-3' Tm: 59.378
Expected PCR Product Length: 315
Expected CelI Cleavage Product Sizes: 131, 184
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr2:64716755-64716816

Rank: 41	Homology Score: 2	RVD Match Score: 3.378	Left Half-Site RVD Score: 6.275	Right Half-Site RVD Score: 6.322
	Genomic Region: Intergenic	Closest Gene: USP9X
Match Type: L26R
	           Left Nuclease
	         TTTTCTGTCACCAATCCT
	5'-TCCAC TCTTCTGACACCACCCCA GAGAGGAGGGGGAGGAGTACTTTGTT ACTGCCTGGTGGAGGTGG ACATC-3'
	3'-AGGTG AGAAGACTGTGGTGGGGT CTCTCCTCCCCCTCCTCATGAAACAA TGACGGACCACCTCCACC TGTAG-5'
	                                                       TGACACCCCACCTCCCCT
	                                                            Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 00X-040907277
Forward Primer: 5'-CTGGTTAGGAGGGTTGAAGGTG-3' Tm: 59.258
Reverse Primer: 5'-GGTCAGAGAAGGCTGAGTAGG-3' Tm: 59.378
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 137, 133
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chrX:40907381-40907442

Rank: 42	Homology Score: 2	RVD Match Score: 3.385	Left Half-Site RVD Score: 6.352	Right Half-Site RVD Score: 6.302
	Genomic Region: Intron	Closest Gene: C20orf112
Match Type: L26R
	           Left Nuclease
	         TTTTCTGTCACCAATCCT
	5'-TCTCC CTTTCTGTTAACAAACTT CAACAAAACTTACATTAGAGAATAAA AGTGTGGGCTGGGTGTGA TGGCT-3'
	3'-AGAGG GAAAGACAATTGTTTGAA GTTGTTTTGAATGTAATCTCTTATTT TCACACCCGACCCACACT ACCGA-5'
	                                                       TGACACCCCACCTCCCCT
	                                                            Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 020-031067101
Forward Primer: 5'-CTGCTCAGAGACCCAAGGTG-3' Tm: 59.508
Reverse Primer: 5'-TAGAGACAGCGTCTCACTGTGTTG-3' Tm: 59.038
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 135, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr20:31067203-31067264

Rank: 43	Homology Score: 5	RVD Match Score: 3.389	Left Half-Site RVD Score: 6.267	Right Half-Site RVD Score: 6.409
	Genomic Region: Intergenic	Closest Gene: MIR802
Match Type: L21R
	           Left Nuclease
	         TTTTCTGTCACCAATCCT
	5'-CAGAG TCCTTTCCCACCAATCCT   TCATCAGCCTAATCAATACTA    GCAGTGGGGTGGAAGGAA GATGA-3'
	3'-GTCTC AGGAAAGGGTGGTTAGGA   AGTAGTCGGATTAGTTATGAT    CGTCACCCCACCTTCCTT CTACT-5'
	                                                       TGACACCCCACCTCCCCT
	                                                            Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 021-036773953
Forward Primer: 5'-CCAGAGCGGTTTTCCAAGATGC-3' Tm: 59.258
Reverse Primer: 5'-GGATTCACAACACCATGGATGGAC-3' Tm: 59.038
Expected PCR Product Length: 371
Expected CelI Cleavage Product Sizes: 175, 196
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr21:36774098-36774154

Rank: 44	Homology Score: 5	RVD Match Score: 3.391	Left Half-Site RVD Score: 6.47	Right Half-Site RVD Score: 6.223
	Genomic Region: Intergenic	Closest Gene: SND1
Match Type: R16L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-ACCTC CCCCTCCCGCCCCAGAGT      TACGGGTTAAGGAGAA      AGACTTGGTCAGAGAAAA CGTGA-3'
	3'-TGGAG GGGGAGGGCGGGGTCTCA      ATGCCCAATTCCTCTT      TCTGAACCAGTCTCTTTT GCACT-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 007-127776243
Forward Primer: 5'-ATGACAGAAGGGGCGTGGTG-3' Tm: 59.508
Reverse Primer: 5'-AGTGGCGCCTCCTAGAAGCA-3' Tm: 59.508
Expected PCR Product Length: 292
Expected CelI Cleavage Product Sizes: 161, 131
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr7:127776376-127776427

Rank: 45	Homology Score: 5	RVD Match Score: 3.392	Left Half-Site RVD Score: 6.417	Right Half-Site RVD Score: 6.288
	Genomic Region: Promoter	Closest Gene: CHAT
Match Type: R11L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-ACCTC TGCTCCCCACCCCATAGT        GCCAGCAGGAA         TGGGATGGTAACAGAAAG GGAAG-3'
	3'-TGGAG ACGAGGGGTGGGGTATCA        CGGTCGTCCTT         ACCCTACCATTGTCTTTC CCTTC-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 010-050821729
Forward Primer: 5'-GTGGGGGATGCTTTTGTCTGAG-3' Tm: 59.258
Reverse Primer: 5'-AAGTCTCTGCTCGCTGCCCT-3' Tm: 59.508
Expected PCR Product Length: 273
Expected CelI Cleavage Product Sizes: 130, 143
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr10:50821834-50821880

Rank: 46	Homology Score: 2	RVD Match Score: 3.395	Left Half-Site RVD Score: 6.501	Right Half-Site RVD Score: 6.235
	Genomic Region: Intergenic	Closest Gene: IFLTD1
Match Type: L20R
	           Left Nuclease
	         TTTTCTGTCACCAATCCT
	5'-CCAAC TTTTCTCACACAAATTAT    CTCATTTAGTTTTCCGAATA    ACTCTCTGGGGGAGGGGG GAATT-3'
	3'-GGTTG AAAAGAGTGTGTTTAATA    GAGTAAATCAAAAGGCTTAT    TGAGAGACCCCCTCCCCC CTTAA-5'
	                                                       TGACACCCCACCTCCCCT
	                                                            Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 012-025903897
Forward Primer: 5'-CCAGCATCTTGTGGCTGAGTAG-3' Tm: 59.258
Reverse Primer: 5'-GGGAAGATGACAGTGTTCTTGACAGG-3' Tm: 60.578
Expected PCR Product Length: 290
Expected CelI Cleavage Product Sizes: 131, 159
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr12:25903997-25904052

Rank: 47	Homology Score: 5	RVD Match Score: 3.396	Left Half-Site RVD Score: 5.513	Right Half-Site RVD Score: 7.23
	Genomic Region: Intergenic	Closest Gene: CLINT1
Match Type: L15R
	           Left Nuclease
	         TTTTCTGTCACCAATCCT
	5'-TGCAC TTTTCAGCCCCCACTCCT      CCTTTATGAAGAGAA       CCTGTGTGCTGGTGGGAA GAGCT-3'
	3'-ACGTG AAAAGTCGGGGGTGAGGA      GGAAATACTTCTCTT       GGACACACGACCACCCTT CTCGA-5'
	                                                       TGACACCCCACCTCCCCT
	                                                            Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 005-157580667
Forward Primer: 5'-GTAATCCAGGGTCTCTGTTTGTCC-3' Tm: 59.038
Reverse Primer: 5'-GTCTTTGGGTGGCATTTGTGAGG-3' Tm: 59.148
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 133, 137
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr5:157580773-157580823

Rank: 48	Homology Score: 2	RVD Match Score: 3.396	Left Half-Site RVD Score: 7.07	Right Half-Site RVD Score: 5.74
	Genomic Region: Intergenic	Closest Gene: ZNRF2
Match Type: L12R
	           Left Nuclease
	         TTTTCTGTCACCAATCCT
	5'-TCCCA TCCTCTGTCATCTATCCC        TACAATCTGCAG        CCTGTGTGGTGTGGGGGG CAGGA-3'
	3'-AGGGT AGGAGACAGTAGATAGGG        ATGTTAGACGTC        GGACACACCACACCCCCC GTCCT-5'
	                                                       TGACACCCCACCTCCCCT
	                                                            Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 007-030310150
Forward Primer: 5'-CTGCTGCCAAAAGCTACCCATG-3' Tm: 59.258
Reverse Primer: 5'-GAGCTCCAGTTTCCTGCACTGT-3' Tm: 59.258
Expected PCR Product Length: 386
Expected CelI Cleavage Product Sizes: 130, 256
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr7:30310251-30310298

Rank: 49	Homology Score: 2	RVD Match Score: 3.401	Left Half-Site RVD Score: 7.533	Right Half-Site RVD Score: 5.395
	Genomic Region: Intergenic	Closest Gene: IQUB
Match Type: L24R
	           Left Nuclease
	         TTTTCTGTCACCAATCCT
	5'-TGTTT TCTTCTGTCGTCCATGCT  GTAAGCTTTGTAGAACCCTAGCCT  TGTGTTGGGAGGAGGGGG AAGAG-3'
	3'-ACAAA AGAAGACAGCAGGTACGA  CATTCGAAACATCTTGGGATCGGA  ACACAACCCTCCTCCCCC TTCTC-5'
	                                                       TGACACCCCACCTCCCCT
	                                                            Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 007-123033818
Forward Primer: 5'-CTACCCCTATGCAGGTTTGCTGA-3' Tm: 59.148
Reverse Primer: 5'-CTTCCCCCAACTCTCACAGG-3' Tm: 59.508
Expected PCR Product Length: 311
Expected CelI Cleavage Product Sizes: 155, 156
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr7:123033941-123034000

Rank: 50	Homology Score: 5	RVD Match Score: 3.406	Left Half-Site RVD Score: 6.265	Right Half-Site RVD Score: 6.54
	Genomic Region: Intron	Closest Gene: ARID1B
Match Type: L22R
	           Left Nuclease
	         TTTTCTGTCACCAATCCT
	5'-AGGGT CTTTCTGAAATCAAGCCT   TGCAAGTGATGTTTCCAAGGAG   GCTGTCGGCTGGAGCGGA GCCTG-3'
	3'-TCCCA GAAAGACTTTAGTTCGGA   ACGTTCACTACAAAGGTTCCTC   CGACAGCCGACCTCGCCT CGGAC-5'
	                                                       TGACACCCCACCTCCCCT
	                                                            Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 006-157508829
Forward Primer: 5'-GCCAGCCTCCTCTATTAATGGAGT-3' Tm: 59.038
Reverse Primer: 5'-GAGTAAAGGAAGAAAGCCGTCTTTTGCAG-3' Tm: 60.178
Expected PCR Product Length: 320
Expected CelI Cleavage Product Sizes: 178, 142
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr6:157508976-157509033

Rank: 51	Homology Score: 2	RVD Match Score: 3.409	Left Half-Site RVD Score: 6.349	Right Half-Site RVD Score: 6.482
	Genomic Region: Intron	Closest Gene: CCDC64
Match Type: L11R
	           Left Nuclease
	         TTTTCTGTCACCAATCCT
	5'-GGCAT CCCTCTGTCACCAGTGCT        GAGACCCCTGG         ACTGTGGTGGTGATGTGA GATCC-3'
	3'-CCGTA GGGAGACAGTGGTCACGA        CTCTGGGGACC         TGACACCACCACTACACT CTAGG-5'
	                                                       TGACACCCCACCTCCCCT
	                                                            Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 012-120471484
Forward Primer: 5'-GCCGGCTGAGAGTTCTTGCT-3' Tm: 59.508
Reverse Primer: 5'-GTATGCCTGTATCTGGCTGTGTTG-3' Tm: 59.038
Expected PCR Product Length: 359
Expected CelI Cleavage Product Sizes: 224, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr12:120471683-120471729

Rank: 52	Homology Score: 2	RVD Match Score: 3.41	Left Half-Site RVD Score: 6.246	Right Half-Site RVD Score: 6.603
	Genomic Region: Intron	Closest Gene: MEGF11
Match Type: R16L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-CCAGT TCCCCTCCTCACTCCAAT      CCCCAGCATCACTGTG      AGGAATGGGGACAACAAG GACAG-3'
	3'-GGTCA AGGGGAGGAGTGAGGTTA      GGGGTCGTAGTGACAC      TCCTTACCCCTGTTGTTC CTGTC-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 015-066339107
Forward Primer: 5'-CACACAGTAGGCTTTGCTGGC-3' Tm: 59.378
Reverse Primer: 5'-GGTGAACTTCATGGGATGTGCTG-3' Tm: 59.148
Expected PCR Product Length: 274
Expected CelI Cleavage Product Sizes: 131, 143
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr15:66339210-66339261

Rank: 53	Homology Score: 2	RVD Match Score: 3.41	Left Half-Site RVD Score: 5.997	Right Half-Site RVD Score: 6.886
	Genomic Region: Promoter	Closest Gene: ODF4
Match Type: R20L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-TCACC TCCCATCACCACCACACT    GGGGAGCAAATTTCAGCATG    AGGTTTGGTGAAGACAAA TTAAC-3'
	3'-AGTGG AGGGTAGTGGTGGTGTGA    CCCCTCGTTTAAAGTCGTAC    TCCAAACCACTTCTGTTT AATTG-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 017-008242168
Forward Primer: 5'-CAGCTATTGCGGGGAGCTAATG-3' Tm: 59.258
Reverse Primer: 5'-AGGCCACAGCTCAGAACTGTGGA-3' Tm: 61.088
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 136, 134
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr17:8242274-8242329

Rank: 54	Homology Score: 5	RVD Match Score: 3.411	Left Half-Site RVD Score: 7.369	Right Half-Site RVD Score: 5.492
	Genomic Region: Intergenic	Closest Gene: SMAD3
Match Type: R13L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-TGTTC TTCCACCCAGCCCTCAGT       GCTCTGCTGAGGC        AGGATGGGTGGCTGGAAA AATGG-3'
	3'-ACAAG AAGGTGGGTCGGGAGTCA       CGAGACGACTCCG        TCCTACCCACCGACCTTT TTACC-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 015-067305574
Forward Primer: 5'-CCCCACCTTGCTGAATGGAC-3' Tm: 59.508
Reverse Primer: 5'-CCAGTCCTTGATCTCTTCCTGAGT-3' Tm: 59.038
Expected PCR Product Length: 275
Expected CelI Cleavage Product Sizes: 142, 133
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr15:67305690-67305738

Rank: 55	Homology Score: 2	RVD Match Score: 3.411	Left Half-Site RVD Score: 6.057	Right Half-Site RVD Score: 6.826
	Genomic Region: Intergenic	Closest Gene: CREM
Match Type: R24L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-ATGTC CCTGCCCCACCCCCCAGT  TTCAAGAGTGGATCTGATCAGTGT  GTGGTGGGAGACAGAAAA TTGGG-3'
	3'-TACAG GGACGGGGTGGGGGGTCA  AAGTTCTCACCTAGACTAGTCACA  CACCACCCTCTGTCTTTT AACCC-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 010-035501885
Forward Primer: 5'-CAGGATGCTGTGTCCCCTCA-3' Tm: 59.508
Reverse Primer: 5'-GAGCTCCGACTCTGAAGGAAG-3' Tm: 59.378
Expected PCR Product Length: 303
Expected CelI Cleavage Product Sizes: 131, 172
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr10:35501984-35502043

Rank: 56	Homology Score: 2	RVD Match Score: 3.412	Left Half-Site RVD Score: 6.479	Right Half-Site RVD Score: 6.371
	Genomic Region: Intron	Closest Gene: ADCK2
Match Type: R24L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-TGGGC CCGTCTCCAGCCCACAGA  AAGGCTTCAGTCTGGAGAGAGGAT  GGGATTTGTGTCTGTAAA ACTGT-3'
	3'-ACCCG GGCAGAGGTCGGGTGTCT  TTCCGAAGTCAGACCTCTCTCCTA  CCCTAAACACAGACATTT TGACA-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 007-140379038
Forward Primer: 5'-TCTGCGCCCCTTTGTCACCA-3' Tm: 59.508
Reverse Primer: 5'-CCTCTAAGGCAGGTCACCAAC-3' Tm: 59.378
Expected PCR Product Length: 299
Expected CelI Cleavage Product Sizes: 132, 167
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr7:140379138-140379197

Rank: 57	Homology Score: 2	RVD Match Score: 3.412	Left Half-Site RVD Score: 6.17	Right Half-Site RVD Score: 6.707
	Genomic Region: Intergenic	Closest Gene: MIR4643
Match Type: R11L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-GACCC TCCGCACCACCCCTCAAC        ACCTCTCCCAT         ATGAGGGGTGACAGCTAA TTGTT-3'
	3'-CTGGG AGGCGTGGTGGGGAGTTG        TGGAGAGGGTA         TACTCCCCACTGTCGATT AACAA-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 006-092651079
Forward Primer: 5'-CAGCAAGGAGGCCAAGAACTCA-3' Tm: 59.258
Reverse Primer: 5'-GTGTGTGGGCCTCAAGTTTTGTAC-3' Tm: 59.038
Expected PCR Product Length: 352
Expected CelI Cleavage Product Sizes: 151, 201
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr6:92651205-92651251

Rank: 58	Homology Score: 10	RVD Match Score: 3.414	Left Half-Site RVD Score: 5.315	Right Half-Site RVD Score: 7.738
	Genomic Region: Intergenic	Closest Gene: FOXP1
Match Type: R20L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-TGAAA TGCCCCCCACCCCAGAGT    ATATTCATTAGGACTCTTTG    ATTATAAGAGACAGAAAA TCAAC-3'
	3'-ACTTT ACGGGGGGTGGGGTCTCA    TATAAGTAATCCTGAGAAAC    TAATATTCTCTGTCTTTT AGTTG-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 003-070995805
Forward Primer: 5'-GCCGTTGCTTTTGCCTTGAACATC-3' Tm: 59.038
Reverse Primer: 5'-TACTTGAGTCCCTGGATCTGGC-3' Tm: 59.258
Expected PCR Product Length: 306
Expected CelI Cleavage Product Sizes: 176, 130
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr3:70995951-70996006

Rank: 59	Homology Score: 10	RVD Match Score: 3.415	Left Half-Site RVD Score: 5.371	Right Half-Site RVD Score: 7.681
	Genomic Region: Intron	Closest Gene: MYT1L
Match Type: R15L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-GAATG TTCCCTCCAACCCAGAGT      GCTGAGAAGGCTGGC       AGGATAGAGGCCAGAACA TGTCT-3'
	3'-CTTAC AAGGGAGGTTGGGTCTCA      CGACTCTTCCGACCG       TCCTATCTCCGGTCTTGT ACAGA-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 002-001827851
Forward Primer: 5'-GGGGGTTGGGGAACGTTTTTAG-3' Tm: 59.258
Reverse Primer: 5'-TCAGGACGAGAGCAGCTCAAATG-3' Tm: 59.148
Expected PCR Product Length: 446
Expected CelI Cleavage Product Sizes: 315, 131
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr2:1828139-1828189

Rank: 60	Homology Score: 5	RVD Match Score: 3.418	Left Half-Site RVD Score: 6.211	Right Half-Site RVD Score: 6.677
	Genomic Region: Intron	Closest Gene: KIAA1217
Match Type: L20R
	           Left Nuclease
	         TTTTCTGTCACCAATCCT
	5'-GACAT TTTTCTTTTATCCATCCT    ACAGCACTGCACAATTATTG    ATTGTGGCTTGGAAGCGA AATGA-3'
	3'-CTGTA AAAAGAAAATAGGTAGGA    TGTCGTGACGTGTTAATAAC    TAACACCGAACCTTCGCT TTACT-5'
	                                                       TGACACCCCACCTCCCCT
	                                                            Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 010-024751913
Forward Primer: 5'-GGTGGATGTGCAGAGATGGAG-3' Tm: 59.378
Reverse Primer: 5'-CTCTACTGAGCCCTCCAGTG-3' Tm: 59.508
Expected PCR Product Length: 343
Expected CelI Cleavage Product Sizes: 208, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr10:24752091-24752146

Rank: 61	Homology Score: 2	RVD Match Score: 3.42	Left Half-Site RVD Score: 6.697	Right Half-Site RVD Score: 6.234
	Genomic Region: Intron	Closest Gene: SNCA
Match Type: L18R
	           Left Nuclease
	         TTTTCTGTCACCAATCCT
	5'-TTCAA TTTTCTATTATCAATTCC     TAGTCTGCTCATAATGCC     ATTTTGGGGTGGGGAGCA AAGGG-3'
	3'-AAGTT AAAAGATAATAGTTAAGG     ATCAGACGAGTATTACGG     TAAAACCCCACCCCTCGT TTCCC-5'
	                                                       TGACACCCCACCTCCCCT
	                                                            Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 004-090656661
Forward Primer: 5'-CAGGGGAGTGTGTACATAGTGTAG-3' Tm: 59.038
Reverse Primer: 5'-CCAGAGTTGTTCCTCTCCACATAG-3' Tm: 59.038
Expected PCR Product Length: 359
Expected CelI Cleavage Product Sizes: 169, 190
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr4:90656801-90656854

Rank: 62	Homology Score: 2	RVD Match Score: 3.426	Left Half-Site RVD Score: 6.986	Right Half-Site RVD Score: 6.025
	Genomic Region: Intron	Closest Gene: CDKAL1
Match Type: L17R
	           Left Nuclease
	         TTTTCTGTCACCAATCCT
	5'-TCCCC TTTTATGTCAACAATTAA     AAAATGTTATATACAGT      ATTGTTGGGTGTAGGTAA GAAAA-3'
	3'-AGGGG AAAATACAGTTGTTAATT     TTTTACAATATATGTCA      TAACAACCCACATCCATT CTTTT-5'
	                                                       TGACACCCCACCTCCCCT
	                                                            Right Nuclease
Warning! Primer pair was generated using suboptimal parameters. 
Primer Index: 006-021174562
Forward Primer: 5'-GTCGGTATAATCTTAGTCAAAAGTTCAACAGGG-3' Tm: 59.758
Reverse Primer: 5'-TAGTTAAATTGCCCTCCAAAATGGCCTTATCAATG-3' Tm: 59.578
Expected PCR Product Length: 406
Expected CelI Cleavage Product Sizes: 275, 131
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr6:21174809-21174861

Rank: 63	Homology Score: 2	RVD Match Score: 3.439	Left Half-Site RVD Score: 7.46	Right Half-Site RVD Score: 5.708
	Genomic Region: Intron	Closest Gene: CFHR5
Match Type: L19R
	           Left Nuclease
	         TTTTCTGTCACCAATCCT
	5'-AGTTA TTTACTTTTACCTTTCCT    CTTTAAGTGACAATTGAAT     ATTAAGGGGTGGAGGAGG AACCT-3'
	3'-TCAAT AAATGAAAATGGAAAGGA    GAAATTCACTGTTAACTTA     TAATTCCCCACCTCCTCC TTGGA-5'
	                                                       TGACACCCCACCTCCCCT
	                                                            Right Nuclease
Warning! Primer pair was generated using suboptimal parameters. 
Primer Index: 001-196973362
Forward Primer: 5'-GCAGAAGAGTGTGTCTGCAATTCATTTTCAGT-3' Tm: 59.848
Reverse Primer: 5'-CACAATATGCAGTAGACTCTGGAAAACAGC-3' Tm: 60.058
Expected PCR Product Length: 447
Expected CelI Cleavage Product Sizes: 152, 295
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr1:196973485-196973539

Rank: 64	Homology Score: 2	RVD Match Score: 3.443	Left Half-Site RVD Score: 6.951	Right Half-Site RVD Score: 6.173
	Genomic Region: Intron	Closest Gene: MECR
Match Type: L11R
	           Left Nuclease
	         TTTTCTGTCACCAATCCT
	5'-GGGGC TTAGCAGTCCCTAATCCT        CTTCTCAAGAG         ACTCTGGGTGGGAGGGAG GCAGC-3'
	3'-CCCCG AATCGTCAGGGATTAGGA        GAAGAGTTCTC         TGAGACCCACCCTCCCTC CGTCG-5'
	                                                       TGACACCCCACCTCCCCT
	                                                            Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 001-029537012
Forward Primer: 5'-TGCACCAAATGCTTAGTGCAGGG-3' Tm: 59.148
Reverse Primer: 5'-GTCACACACTAGTGAGTGGTAGAG-3' Tm: 59.038
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 135, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr1:29537122-29537168

Rank: 65	Homology Score: 5	RVD Match Score: 3.445	Left Half-Site RVD Score: 5.52	Right Half-Site RVD Score: 7.746
	Genomic Region: Intergenic	Closest Gene: NUF2
Match Type: R11L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-TTGCT TGACCTCCACACCACACT        CCATCTCTCTT         GGGATAAGTGCCAAAAAA GAGGT-3'
	3'-AACGA ACTGGAGGTGTGGTGTGA        GGTAGAGAGAA         CCCTATTCACGGTTTTTT CTCCA-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 001-163803301
Forward Primer: 5'-GGTTAAGTGCTTGACCACTGCTG-3' Tm: 59.148
Reverse Primer: 5'-CACCATGGCACATGTCTACTTGAG-3' Tm: 59.038
Expected PCR Product Length: 336
Expected CelI Cleavage Product Sizes: 139, 197
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr1:163803415-163803461

Rank: 66	Homology Score: 5	RVD Match Score: 3.445	Left Half-Site RVD Score: 6.803	Right Half-Site RVD Score: 6.322
	Genomic Region: Intron	Closest Gene: NFATC2
Match Type: L25R
	           Left Nuclease
	         TTTTCTGTCACCAATCCT
	5'-TCGGG TTTGCTGTCACAACAACT GCAGGATGACCCGGTGCTGGGCAGC  ACTGCTGGCTGGAGGTGA GAAAT-3'
	3'-AGCCC AAACGACAGTGTTGTTGA CGTCCTACTGGGCCACGACCCGTCG  TGACGACCGACCTCCACT CTTTA-5'
	                                                       TGACACCCCACCTCCCCT
	                                                            Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 020-050029460
Forward Primer: 5'-AGAAGCACAGGATGAGGCCG-3' Tm: 59.508
Reverse Primer: 5'-CAGTGGATTGACAGAGGCAGATG-3' Tm: 59.148
Expected PCR Product Length: 336
Expected CelI Cleavage Product Sizes: 205, 131
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr20:50029633-50029693

Rank: 67	Homology Score: 2	RVD Match Score: 3.447	Left Half-Site RVD Score: 5.466	Right Half-Site RVD Score: 7.833
	Genomic Region: Intron	Closest Gene: PINX1
Match Type: R16L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-CCCAC CCCCCCCCCCACCAAAGT      AGAACAAAGATACAGT      AAAATTGGAGACAAAAGA AAAAT-3'
	3'-GGGTG GGGGGGGGGGTGGTTTCA      TCTTGTTTCTATGTCA      TTTTAACCTCTGTTTTCT TTTTA-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 008-010664694
Forward Primer: 5'-GTATCCGTGAGACAAGAACAGGAC-3' Tm: 59.038
Reverse Primer: 5'-CCAGGCACTTGGTAGAACTTTCAG-3' Tm: 59.038
Expected PCR Product Length: 343
Expected CelI Cleavage Product Sizes: 173, 170
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr8:10664839-10664890

Rank: 68	Homology Score: 5	RVD Match Score: 3.449	Left Half-Site RVD Score: 7.012	Right Half-Site RVD Score: 6.165
	Genomic Region: Intron	Closest Gene: PTPRN2
Match Type: L25R
	           Left Nuclease
	         TTTTCTGTCACCAATCCT
	5'-AGAGA TCTGCTGTGACCACACCT GCAGGGTACTCAGGCCACCTTTCAT  ACTGGGGTGAGGAGAGGA AGTAA-3'
	3'-TCTCT AGACGACACTGGTGTGGA CGTCCCATGAGTCCGGTGGAAAGTA  TGACCCCACTCCTCTCCT TCATT-5'
	                                                       TGACACCCCACCTCCCCT
	                                                            Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 007-158192599
Forward Primer: 5'-TAGTCCCCAGGCACTTGCAG-3' Tm: 59.508
Reverse Primer: 5'-GCCTCGTAAGTTTAGGCATCCTC-3' Tm: 59.148
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 137, 133
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr7:158192704-158192764

Rank: 69	Homology Score: 5	RVD Match Score: 3.455	Left Half-Site RVD Score: 5.689	Right Half-Site RVD Score: 7.621
	Genomic Region: Intron	Closest Gene: STARD7
Match Type: R17L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-CTCCG TGCCCTCCACCTCACCCT     ACCCCCTGCAACATTTA      AAGAAAGGTTACAGATAA GAAAC-3'
	3'-GAGGC ACGGGAGGTGGAGTGGGA     TGGGGGACGTTGTAAAT      TTCTTTCCAATGTCTATT CTTTG-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 002-096866458
Forward Primer: 5'-CGCCGAAACTGGCCTCAAATTCC-3' Tm: 61.088
Reverse Primer: 5'-CTGCCTAGCAGTTACCTTTGTGG-3' Tm: 59.148
Expected PCR Product Length: 295
Expected CelI Cleavage Product Sizes: 130, 165
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr2:96866560-96866612

Rank: 70	Homology Score: 2	RVD Match Score: 3.456	Left Half-Site RVD Score: 6.956	Right Half-Site RVD Score: 6.222
	Genomic Region: Intergenic	Closest Gene: SS18
Match Type: R13L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-AACCC TCAGCTCCACCTCCCAGA       GGTATCTGGGCTC        AGGATGGGAGACAGAGCG CTAAA-3'
	3'-TTGGG AGTCGAGGTGGAGGGTCT       CCATAGACCCGAG        TCCTACCCTCTGTCTCGC GATTT-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 018-023479636
Forward Primer: 5'-CCTGAGCAACAGAGTGAAACTCC-3' Tm: 59.148
Reverse Primer: 5'-GGGTTTCCCTCAGGCATGTG-3' Tm: 59.508
Expected PCR Product Length: 302
Expected CelI Cleavage Product Sizes: 171, 131
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr18:23479781-23479829

Rank: 71	Homology Score: 2	RVD Match Score: 3.459	Left Half-Site RVD Score: 7.07	Right Half-Site RVD Score: 6.185
	Genomic Region: Intergenic	Closest Gene: ZNRF2
Match Type: L14R
	           Left Nuclease
	         TTTTCTGTCACCAATCCT
	5'-TCCCA TCCTCTGTCATCTATCCC       TACAATCTGCAGCC       TGTGTGGTGTGGGGGGCA GGAGA-3'
	3'-AGGGT AGGAGACAGTAGATAGGG       ATGTTAGACGTCGG       ACACACCACACCCCCCGT CCTCT-5'
	                                                       TGACACCCCACCTCCCCT
	                                                            Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 007-030310150
Forward Primer: 5'-CTGCTGCCAAAAGCTACCCATG-3' Tm: 59.258
Reverse Primer: 5'-GAGCTCCAGTTTCCTGCACTGT-3' Tm: 59.258
Expected PCR Product Length: 386
Expected CelI Cleavage Product Sizes: 130, 256
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr7:30310251-30310300

Rank: 72	Homology Score: 2	RVD Match Score: 3.461	Left Half-Site RVD Score: 6.331	Right Half-Site RVD Score: 6.917
	Genomic Region: Intergenic	Closest Gene: CNOT6
Match Type: R16L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-TCCCA CGCCCCCCACCTCACAGG      GGTGAGCACAGGTCTG      AGGTTTGTTGAACGAACA GCTCC-3'
	3'-AGGGT GCGGGGGGTGGAGTGTCC      CCACTCGTGTCCAGAC      TCCAAACAACTTGCTTGT CGAGG-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 005-179890957
Forward Primer: 5'-AAATGGACCCTCATCGTGGGGTG-3' Tm: 61.088
Reverse Primer: 5'-AATGCTGGGATTTGTAGCTGGGG-3' Tm: 59.148
Expected PCR Product Length: 301
Expected CelI Cleavage Product Sizes: 171, 130
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr5:179891100-179891151

Rank: 73	Homology Score: 2	RVD Match Score: 3.462	Left Half-Site RVD Score: 7.398	Right Half-Site RVD Score: 5.833
	Genomic Region: Intron	Closest Gene: ARHGAP23
Match Type: R18L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-CTGAG TCCTCTCCAGGACCCAGT     TTACAGCTGAGGAGACTG     AGGCTTGGAGAGGGAAAG GACTT-3'
	3'-GACTC AGGAGAGGTCCTGGGTCA     AATGTCGACTCCTCTGAC     TCCGAACCTCTCCCTTTC CTGAA-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 017-036596557
Forward Primer: 5'-CCCAGGCTCTGCTTAGGAAC-3' Tm: 59.508
Reverse Primer: 5'-TTCACTGGGTGCCACCAAGG-3' Tm: 59.508
Expected PCR Product Length: 340
Expected CelI Cleavage Product Sizes: 204, 136
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr17:36596732-36596785

Rank: 74	Homology Score: 2	RVD Match Score: 3.463	Left Half-Site RVD Score: 6.616	Right Half-Site RVD Score: 6.626
	Genomic Region: Intergenic	Closest Gene: HAS2-AS1
Match Type: L13R
	           Left Nuclease
	         TTTTCTGTCACCAATCCT
	5'-AGTAC CATTTTGTCATTAATCCT       TTGCATTGGGGGT        TATGTTGGGTGGACTGGA CTTTC-3'
	3'-TCATG GTAAAACAGTAATTAGGA       AACGTAACCCCCA        ATACAACCCACCTGACCT GAAAG-5'
	                                                       TGACACCCCACCTCCCCT
	                                                            Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 008-123011852
Forward Primer: 5'-CCCTGATACCTACCAGCTACTTTG-3' Tm: 59.038
Reverse Primer: 5'-GTCCTGTGTGTCATATGTCTGCTC-3' Tm: 59.038
Expected PCR Product Length: 287
Expected CelI Cleavage Product Sizes: 147, 140
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr8:123011973-123012021

Rank: 75	Homology Score: 5	RVD Match Score: 3.472	Left Half-Site RVD Score: 6.794	Right Half-Site RVD Score: 6.526
	Genomic Region: Intergenic	Closest Gene: MOBP
Match Type: L13R
	           Left Nuclease
	         TTTTCTGTCACCAATCCT
	5'-TAGAT TTTTTTGTAAGCAATCTT       TTGAGACACAAGA        AGTGGGGTGTAGAGAGGA ATTCA-3'
	3'-ATCTA AAAAAACATTCGTTAGAA       AACTCTGTGTTCT        TCACCCCACATCTCTCCT TAAGT-5'
	                                                       TGACACCCCACCTCCCCT
	                                                            Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 003-039683322
Forward Primer: 5'-GGTCTCCAGGGAGCATTATACAG-3' Tm: 59.148
Reverse Primer: 5'-CTAGACACAGAGCCTTCAGTAAGC-3' Tm: 59.038
Expected PCR Product Length: 410
Expected CelI Cleavage Product Sizes: 229, 181
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr3:39683525-39683573

Rank: 76	Homology Score: 2	RVD Match Score: 3.472	Left Half-Site RVD Score: 6.894	Right Half-Site RVD Score: 6.408
	Genomic Region: Intergenic	Closest Gene: NFE2L1
Match Type: R12L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-GAGCC TCTCCACAACTCCCCAGT        CTCCAGTTGGAA        AGGTTTGGTCATATCAAA CAAGA-3'
	3'-CTCGG AGAGGTGTTGAGGGGTCA        GAGGTCAACCTT        TCCAAACCAGTATAGTTT GTTCT-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 017-046139902
Forward Primer: 5'-TGTAACCTGGTTCAGCTCAGTCC-3' Tm: 59.148
Reverse Primer: 5'-ACCGCACCAGGCCTCAAACT-3' Tm: 59.508
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 135, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr17:46140011-46140058

Rank: 77	Homology Score: 2	RVD Match Score: 3.473	Left Half-Site RVD Score: 7.398	Right Half-Site RVD Score: 5.995
	Genomic Region: Intergenic	Closest Gene: COG2
Match Type: L19R
	           Left Nuclease
	         TTTTCTGTCACCAATCCT
	5'-ATCTC TATTTTGACATTAATCCT    TGTCAGCTGTTGTGTCTAA     ACTGTGAAGGGGTGGGGG GAGTG-3'
	3'-TAGAG ATAAAACTGTAATTAGGA    ACAGTCGACAACACAGATT     TGACACTTCCCCACCCCC CTCAC-5'
	                                                       TGACACCCCACCTCCCCT
	                                                            Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 001-230708668
Forward Primer: 5'-GGGCACGCGATTTAACCCTTG-3' Tm: 59.378
Reverse Primer: 5'-CCTCCTAACCAACAGAACAGACC-3' Tm: 59.148
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 132, 138
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr1:230708771-230708825

Rank: 78	Homology Score: 2	RVD Match Score: 3.477	Left Half-Site RVD Score: 6.501	Right Half-Site RVD Score: 6.839
	Genomic Region: Intergenic	Closest Gene: IFLTD1
Match Type: L22R
	           Left Nuclease
	         TTTTCTGTCACCAATCCT
	5'-CCAAC TTTTCTCACACAAATTAT   CTCATTTAGTTTTCCGAATAAC   TCTCTGGGGGAGGGGGGA ATTAA-3'
	3'-GGTTG AAAAGAGTGTGTTTAATA   GAGTAAATCAAAAGGCTTATTG   AGAGACCCCCTCCCCCCT TAATT-5'
	                                                       TGACACCCCACCTCCCCT
	                                                            Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 012-025903897
Forward Primer: 5'-CCAGCATCTTGTGGCTGAGTAG-3' Tm: 59.258
Reverse Primer: 5'-GGGAAGATGACAGTGTTCTTGACAGG-3' Tm: 60.578
Expected PCR Product Length: 290
Expected CelI Cleavage Product Sizes: 131, 159
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr12:25903997-25904054

Rank: 79	Homology Score: 2	RVD Match Score: 3.482	Left Half-Site RVD Score: 6.572	Right Half-Site RVD Score: 6.825
	Genomic Region: Intron	Closest Gene: ANGPT1
Match Type: R12L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-ATCTC TTCCCTGCTCCCCACCAT        TCAGAATTTGTT        GTAATTTGTGAAAGAAAA GTGAA-3'
	3'-TAGAG AAGGGACGAGGGGTGGTA        AGTCTTAAACAA        CATTAAACACTTTCTTTT CACTT-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 008-108496180
Forward Primer: 5'-GATGGGAAAGATGCCTAGGAAGC-3' Tm: 59.148
Reverse Primer: 5'-GGAAACTGAGGGCCACTAAAGTC-3' Tm: 59.148
Expected PCR Product Length: 339
Expected CelI Cleavage Product Sizes: 186, 153
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr8:108496340-108496387

Rank: 80	Homology Score: 5	RVD Match Score: 3.483	Left Half-Site RVD Score: 6.805	Right Half-Site RVD Score: 6.595
	Genomic Region: Intron	Closest Gene: AFF1
Match Type: L21R
	           Left Nuclease
	         TTTTCTGTCACCAATCCT
	5'-TTCTC TATTCTGCCTCAAAACCT   AGTTGCGGTTGTTCCTTGAGC    ACTGTGGCATGGAGCTGA GTTTC-3'
	3'-AAGAG ATAAGACGGAGTTTTGGA   TCAACGCCAACAAGGAACTCG    TGACACCGTACCTCGACT CAAAG-5'
	                                                       TGACACCCCACCTCCCCT
	                                                            Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 004-088055877
Forward Primer: 5'-TGGGGTGTTTGGATTGGGAGG-3' Tm: 59.378
Reverse Primer: 5'-TCAGCTAGCATTTCAGCTCAGGC-3' Tm: 59.148
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 135, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr4:88055982-88056038

Rank: 81	Homology Score: 5	RVD Match Score: 3.484	Left Half-Site RVD Score: 6.521	Right Half-Site RVD Score: 6.873
	Genomic Region: Intron	Closest Gene: TBC1D12
Match Type: L19R
	           Left Nuclease
	         TTTTCTGTCACCAATCCT
	5'-CTTTC TTTTCTGTAGCAAATTCT    AGATCTATTTTTTCTTATT     TCTTTGAGGTGGGGAGGA GTAGT-3'
	3'-GAAAG AAAAGACATCGTTTAAGA    TCTAGATAAAAAAGAATAA     AGAAACTCCACCCCTCCT CATCA-5'
	                                                       TGACACCCCACCTCCCCT
	                                                            Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 010-096170810
Forward Primer: 5'-GGCATGCCAGAGATAGGACTATTC-3' Tm: 59.038
Reverse Primer: 5'-GTGCTACAGTGGCAACCTTCATG-3' Tm: 59.148
Expected PCR Product Length: 330
Expected CelI Cleavage Product Sizes: 191, 139
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr10:96170972-96171026

Rank: 82	Homology Score: 2	RVD Match Score: 3.485	Left Half-Site RVD Score: 6.747	Right Half-Site RVD Score: 6.665
	Genomic Region: Intron	Closest Gene: KIRREL3
Match Type: R13L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-GTGTT TTCACTCACCACCACAGT       CAGGGCTGGCACA        GGGACTGGGGAATGAAAA ATAAA-3'
	3'-CACAA AAGTGAGTGGTGGTGTCA       GTCCCGACCGTGT        CCCTGACCCCTTACTTTT TATTT-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 011-126543310
Forward Primer: 5'-CCAGCAGCTCTGGAAATTGGATC-3' Tm: 59.148
Reverse Primer: 5'-GAGGAAGATCTTACAGACCCAACC-3' Tm: 59.038
Expected PCR Product Length: 345
Expected CelI Cleavage Product Sizes: 187, 158
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr11:126543471-126543519

Rank: 83	Homology Score: 2	RVD Match Score: 3.488	Left Half-Site RVD Score: 6.878	Right Half-Site RVD Score: 6.565
	Genomic Region: Intergenic	Closest Gene: UGT8
Match Type: L22R
	           Left Nuclease
	         TTTTCTGTCACCAATCCT
	5'-TATAG TTTTCTTTCAACATTCAC   TCAGGAAATGATTATGTGTGAG   TATGTGGATTTGAGGGGA AGTTA-3'
	3'-ATATC AAAAGAAAGTTGTAAGTG   AGTCCTTTACTAATACACACTC   ATACACCTAAACTCCCCT TCAAT-5'
	                                                       TGACACCCCACCTCCCCT
	                                                            Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 004-115491385
Forward Primer: 5'-CATGTTGCACAGGCTGAGTACTCT-3' Tm: 59.038
Reverse Primer: 5'-CCCATAGCTCTTTGGTCAGGTC-3' Tm: 59.258
Expected PCR Product Length: 399
Expected CelI Cleavage Product Sizes: 224, 175
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr4:115491578-115491635

Rank: 84	Homology Score: 2	RVD Match Score: 3.49	Left Half-Site RVD Score: 6.944	Right Half-Site RVD Score: 6.494
	Genomic Region: Intron	Closest Gene: GPC4
Match Type: R25L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-TGGAA TCCCCTGCACCCAGCATA CTGCTCTGGATACAGGAGCCATTCA  ATGATTGCTGATAATAAA TTTCT-3'
	3'-ACCTT AGGGGACGTGGGTCGTAT GACGAGACCTATGTCCTCGGTAAGT  TACTAACGACTATTATTT AAAGA-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 00X-132539743
Forward Primer: 5'-CTGCCATAGACTGGGAAACTGGA-3' Tm: 59.148
Reverse Primer: 5'-CCAGAACACAGCTGGGAATGTGGA-3' Tm: 60.908
Expected PCR Product Length: 283
Expected CelI Cleavage Product Sizes: 152, 131
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chrX:132539863-132539923

Rank: 85	Homology Score: 2	RVD Match Score: 3.49	Left Half-Site RVD Score: 6.57	Right Half-Site RVD Score: 6.871
	Genomic Region: Intergenic	Closest Gene: LMO1
Match Type: L22R
	           Left Nuclease
	         TTTTCTGTCACCAATCCT
	5'-ACCTT TATACTGTCCCCAAGCCC   CCACTTATCAAAACACATTCTG   ATTGAGGGGTAGAGAGAA GGGAG-3'
	3'-TGGAA ATATGACAGGGGTTCGGG   GGTGAATAGTTTTGTGTAAGAC   TAACTCCCCATCTCTCTT CCCTC-5'
	                                                       TGACACCCCACCTCCCCT
	                                                            Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 011-008291463
Forward Primer: 5'-GTTTCCTCGGGCAATGAATGAGC-3' Tm: 59.148
Reverse Primer: 5'-GCACAGTCCTCCTTTCTTCACC-3' Tm: 59.258
Expected PCR Product Length: 311
Expected CelI Cleavage Product Sizes: 155, 156
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr11:8291587-8291644

Rank: 86	Homology Score: 2	RVD Match Score: 3.492	Left Half-Site RVD Score: 7.689	Right Half-Site RVD Score: 5.787
	Genomic Region: Intron	Closest Gene: LYPD6B
Match Type: R23L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-CCTTA TCTCCTCCTTCCTAAAGT  AAGGTCTTTAGATATTCTTTTGA   AGTTTTGTTGATAGTAAA CTCCT-3'
	3'-GGAAT AGAGGAGGAAGGATTTCA  TTCCAGAAATCTATAAGAAAACT   TCAAAACAACTATCATTT GAGGA-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 002-149982161
Forward Primer: 5'-GGCATCTTCGAGTACCTTAACTCC-3' Tm: 59.038
Reverse Primer: 5'-CTCTTCTCAAGTTCAAGGGTCAGTTGTAC-3' Tm: 60.178
Expected PCR Product Length: 409
Expected CelI Cleavage Product Sizes: 219, 190
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr2:149982349-149982407

Rank: 87	Homology Score: 2	RVD Match Score: 3.492	Left Half-Site RVD Score: 6.687	Right Half-Site RVD Score: 6.779
	Genomic Region: Intergenic	Closest Gene: POM121L12
Match Type: L23R
	           Left Nuclease
	         TTTTCTGTCACCAATCCT
	5'-CTGAA TTTGCAGCCCCAAATCCT  GCCCACTGAGCAGACTTCTTTCT   ACCTTGGGGTGGGGTTGA AAGCG-3'
	3'-GACTT AAACGTCGGGGTTTAGGA  CGGGTGACTCGTCTGAAGAAAGA   TGGAACCCCACCCCAACT TTCGC-5'
	                                                       TGACACCCCACCTCCCCT
	                                                            Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 007-052309823
Forward Primer: 5'-CCTGAGTCCCTGCTGCATTAATTC-3' Tm: 59.038
Reverse Primer: 5'-ACTCTGTGTCCTCAACGGTGTTC-3' Tm: 59.148
Expected PCR Product Length: 341
Expected CelI Cleavage Product Sizes: 210, 131
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr7:52310002-52310060

Rank: 88	Homology Score: 5	RVD Match Score: 3.494	Left Half-Site RVD Score: 6.765	Right Half-Site RVD Score: 6.717
	Genomic Region: Intron	Closest Gene: LOC100188947
Match Type: R17L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-TCTTC TGCCTGCCACCCCTCAGT     GGAAGCCTGGCAGAGAG      TGGATGGCTGTCAGAAGA TGCTG-3'
	3'-AGAAG ACGGACGGTGGGGAGTCA     CCTTCGGACCGTCTCTC      ACCTACCGACAGTCTTCT ACGAC-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 010-093286041
Forward Primer: 5'-GATTCTGAGCAGGGAGCTTGG-3' Tm: 59.378
Reverse Primer: 5'-ACAGGATGAGCTAGGGCCTGA-3' Tm: 59.378
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 135, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr10:93286148-93286200

Rank: 89	Homology Score: 2	RVD Match Score: 3.497	Left Half-Site RVD Score: 6.825	Right Half-Site RVD Score: 6.682
	Genomic Region: Intron	Closest Gene: C16orf46
Match Type: L17R
	           Left Nuclease
	         TTTTCTGTCACCAATCCT
	5'-GTTTA TTTTCTTTTACCAATAAC     ATGTTTTGCTTCAAAAT      AATGTGAGGTTGAGGTTA GGGAG-3'
	3'-CAAAT AAAAGAAAATGGTTATTG     TACAAAACGAAGTTTTA      TTACACTCCAACTCCAAT CCCTC-5'
	                                                       TGACACCCCACCTCCCCT
	                                                            Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 016-081100645
Forward Primer: 5'-GCCCCCAAAGTGCATGTTTGGA-3' Tm: 59.258
Reverse Primer: 5'-GAGCTCTCCGATAACATCATGGC-3' Tm: 59.148
Expected PCR Product Length: 420
Expected CelI Cleavage Product Sizes: 244, 176
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr16:81100861-81100913

Rank: 90	Homology Score: 5	RVD Match Score: 3.498	Left Half-Site RVD Score: 6.409	Right Half-Site RVD Score: 7.134
	Genomic Region: Exon	Closest Gene: CDKAL1
Match Type: R16L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-TTCCC TCCCCTTCTCTCCACAGA      TTGGTGAAAGACAACA      AGTGTTAGTAACAGAAGA ATCTT-3'
	3'-AAGGG AGGGGAAGAGAGGTGTCT      AACCACTTTCTGTTGT      TCACAATCATTGTCTTCT TAGAA-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 006-021198081
Forward Primer: 5'-CCGTGATTTGAACAGGGTTCGC-3' Tm: 59.258
Reverse Primer: 5'-CTCTCCCCTTTAAATGCCCTTTGC-3' Tm: 59.038
Expected PCR Product Length: 341
Expected CelI Cleavage Product Sizes: 182, 159
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr6:21198235-21198286

Rank: 91	Homology Score: 2	RVD Match Score: 3.499	Left Half-Site RVD Score: 7.07	Right Half-Site RVD Score: 6.471
	Genomic Region: Intergenic	Closest Gene: ZNRF2
Match Type: L19R
	           Left Nuclease
	         TTTTCTGTCACCAATCCT
	5'-TCCCA TCCTCTGTCATCTATCCC    TACAATCTGCAGCCTGTGT     GGTGTGGGGGGCAGGAGA AGATT-3'
	3'-AGGGT AGGAGACAGTAGATAGGG    ATGTTAGACGTCGGACACA     CCACACCCCCCGTCCTCT TCTAA-5'
	                                                       TGACACCCCACCTCCCCT
	                                                            Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 007-030310150
Forward Primer: 5'-CTGCTGCCAAAAGCTACCCATG-3' Tm: 59.258
Reverse Primer: 5'-GAGCTCCAGTTTCCTGCACTGT-3' Tm: 59.258
Expected PCR Product Length: 386
Expected CelI Cleavage Product Sizes: 130, 256
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr7:30310251-30310305

Rank: 92	Homology Score: 2	RVD Match Score: 3.5	Left Half-Site RVD Score: 6.804	Right Half-Site RVD Score: 6.717
	Genomic Region: Intergenic	Closest Gene: NKX6-1
Match Type: R23L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-ACCAG TCGACACCACCCCACATG  CATTTTCTTTTTGATCCTGATCT   TGCATGGGTGACTGATAA TGAAA-3'
	3'-TGGTC AGCTGTGGTGGGGTGTAC  GTAAAAGAAAAACTAGGACTAGA   ACGTACCCACTGACTATT ACTTT-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 004-085429094
Forward Primer: 5'-GTCTCCCTTGCCAGACTATAACC-3' Tm: 59.148
Reverse Primer: 5'-CATGGCCAAGAGCTGTGCTTTC-3' Tm: 59.258
Expected PCR Product Length: 342
Expected CelI Cleavage Product Sizes: 146, 196
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr4:85429209-85429267

Rank: 93	Homology Score: 5	RVD Match Score: 3.501	Left Half-Site RVD Score: 6.21	Right Half-Site RVD Score: 7.316
	Genomic Region: Intergenic	Closest Gene: NEK7
Match Type: L25R
	           Left Nuclease
	         TTTTCTGTCACCAATCCT
	5'-GCTGA TTTCCTGACACCAAGGCT CCTCAGCTGAGGCTCCCACCTGCAA  GCTGTGAGCTGGAGATGA ATATC-3'
	3'-CGACT AAAGGACTGTGGTTCCGA GGAGTCGACTCCGAGGGTGGACGTT  CGACACTCGACCTCTACT TATAG-5'
	                                                       TGACACCCCACCTCCCCT
	                                                            Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 001-198360906
Forward Primer: 5'-GGTTTTATAGCCCTTCTCCTGCC-3' Tm: 59.148
Reverse Primer: 5'-GTTGTGGGATTTGAGCTGGTGG-3' Tm: 59.258
Expected PCR Product Length: 300
Expected CelI Cleavage Product Sizes: 130, 170
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr1:198361004-198361064

Rank: 94	Homology Score: 2	RVD Match Score: 3.501	Left Half-Site RVD Score: 6.551	Right Half-Site RVD Score: 7.004
	Genomic Region: Intergenic	Closest Gene: ADCK2
Match Type: R22L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-TCTAG TCCCCTGCATCCCGCAAC   ATTTCTTGGCGAGCCAGCCAGG   AGGAGTGGAGACGGCAGA TCTAC-3'
	3'-AGATC AGGGGACGTAGGGCGTTG   TAAAGAACCGCTCGGTCGGTCC   TCCTCACCTCTGCCGTCT AGATG-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 007-140355184
Forward Primer: 5'-CCCACAAGGGCCTTATTTTCAGTC-3' Tm: 59.038
Reverse Primer: 5'-GAGAGCTGATTTCACCCCTCTAG-3' Tm: 59.148
Expected PCR Product Length: 369
Expected CelI Cleavage Product Sizes: 236, 133
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr7:140355389-140355446

Rank: 95	Homology Score: 2	RVD Match Score: 3.502	Left Half-Site RVD Score: 7.159	Right Half-Site RVD Score: 6.371
	Genomic Region: Intergenic	Closest Gene: FAM9C
Match Type: R22L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-GAATT TCCCTTCTATCCCCCAGA   TCCACACTTCTCTATTCAAGTC   AGGGTTTGTGACAGGGTA GCCTA-3'
	3'-CTTAA AGGGAAGATAGGGGGTCT   AGGTGTGAAGAGATAAGTTCAG   TCCCAAACACTGTCCCAT CGGAT-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 00X-013108270
Forward Primer: 5'-GCCATTTTGGCAGAGCCACTGA-3' Tm: 59.258
Reverse Primer: 5'-GCATGTTGTGCACGAAATGACCTC-3' Tm: 59.038
Expected PCR Product Length: 271
Expected CelI Cleavage Product Sizes: 131, 140
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chrX:13108370-13108427

Rank: 96	Homology Score: 2	RVD Match Score: 3.502	Left Half-Site RVD Score: 7.134	Right Half-Site RVD Score: 6.437
	Genomic Region: Intron	Closest Gene: VIT
Match Type: L21R
	           Left Nuclease
	         TTTTCTGTCACCAATCCT
	5'-ACACG TTCTTTGTAATCAACCCT   AGGATATCAAAATTGGAAACA    GTTGTGGGATGAAGGGAA CTGTT-3'
	3'-TGTGC AAGAAACATTAGTTGGGA   TCCTATAGTTTTAACCTTTGT    CAACACCCTACTTCCCTT GACAA-5'
	                                                       TGACACCCCACCTCCCCT
	                                                            Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 002-037018108
Forward Primer: 5'-TTTCAGCTGCATCCCGGACC-3' Tm: 59.508
Reverse Primer: 5'-AGGAGATGCGGCCAAGTCAG-3' Tm: 59.508
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 135, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr2:37018213-37018269

Rank: 97	Homology Score: 2	RVD Match Score: 3.502	Left Half-Site RVD Score: 6.564	Right Half-Site RVD Score: 6.997
	Genomic Region: Intergenic	Closest Gene: HERC6
Match Type: R14L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-TGCCC TCCCATCCCCACCCCAGG       GGGCTTCCATGTCA       CAGATAGGTGAGAGATAA ATGGT-3'
	3'-ACGGG AGGGTAGGGGTGGGGTCC       CCCGAAGGTACAGT       GTCTATCCACTCTCTATT TACCA-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 004-089270564
Forward Primer: 5'-GTGGTTGGGGCACAGCTTAG-3' Tm: 59.508
Reverse Primer: 5'-CACACCTGCCTCCCATTCTATTC-3' Tm: 59.148
Expected PCR Product Length: 298
Expected CelI Cleavage Product Sizes: 165, 133
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr4:89270702-89270751

Rank: 98	Homology Score: 2	RVD Match Score: 3.502	Left Half-Site RVD Score: 6.926	Right Half-Site RVD Score: 6.612
	Genomic Region: Intron	Closest Gene: MAPT
Match Type: R24L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-TTCCT TACCTCCCACCCCACTGC  ATCTAAGTCACTGCCTGAGAAAAC  AGGATTGAGGAAAGGAGA AAGGA-3'
	3'-AAGGA ATGGAGGGTGGGGTGACG  TAGATTCAGTGACGGACTCTTTTG  TCCTAACTCCTTTCCTCT TTCCT-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 017-044029096
Forward Primer: 5'-GGACGGACTCAGGAGGCAAT-3' Tm: 59.508
Reverse Primer: 5'-CACACCAAGGCTGTTTTCCTCAGA-3' Tm: 59.038
Expected PCR Product Length: 266
Expected CelI Cleavage Product Sizes: 136, 130
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr17:44029200-44029259

Rank: 99	Homology Score: 2	RVD Match Score: 3.502	Left Half-Site RVD Score: 6.612	Right Half-Site RVD Score: 6.926
	Genomic Region: Intron	Closest Gene: MAPT
Match Type: L24R
	           Left Nuclease
	         TTTTCTGTCACCAATCCT
	5'-TCCTT TCTCCTTTCCTCAATCCT  GTTTTCTCAGGCAGTGACTTAGAT  GCAGTGGGGTGGGAGGTA AGGAA-3'
	3'-AGGAA AGAGGAAAGGAGTTAGGA  CAAAAGAGTCCGTCACTGAATCTA  CGTCACCCCACCCTCCAT TCCTT-5'
	                                                       TGACACCCCACCTCCCCT
	                                                            Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: ap1-000838311
Forward Primer: 5'-ACCAAGGCTGTTTTCCTCAGACC-3' Tm: 59.148
Reverse Primer: 5'-AACCACAGGGACGGACTCAG-3' Tm: 59.508
Expected PCR Product Length: 271
Expected CelI Cleavage Product Sizes: 131, 140
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr17_ctg5_hap1:838410-838469

Rank: 100	Homology Score: 2	RVD Match Score: 3.504	Left Half-Site RVD Score: 7.986	Right Half-Site RVD Score: 5.731
	Genomic Region: Intergenic	Closest Gene: PRKRIR
Match Type: L21R
	           Left Nuclease
	         TTTTCTGTCACCAATCCT
	5'-TTCCT TGTTCTGTAGCCAATGGT   GCAATTATTTGTAACCCAGTG    GCTGGGAGGTGGAGGGTG GCTCT-3'
	3'-AAGGA ACAAGACATCGGTTACCA   CGTTAATAAACATTGGGTCAC    CGACCCTCCACCTCCCAC CGAGA-5'
	                                                       TGACACCCCACCTCCCCT
	                                                            Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 011-076020841
Forward Primer: 5'-AAGGAGACTCCCCACATGTTGC-3' Tm: 59.258
Reverse Primer: 5'-GGTTACAGCTGCTTCTGCCAC-3' Tm: 59.378
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 131, 139
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr11:76020942-76020998

Plate format Oligo Order Form
Well
Name
Sequence
5' mods
Internal
Notes
Primer Plate #1
A1
019-055627003F
CCAGGAAATGGGGGTGTGTC
Hockemeyer_AAVS1_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
A2
019-055627003R
GTCCACTTCAGGACAGCATGTTTG
Hockemeyer_AAVS1_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
B1
003-051729704F
GGAGATGAACTCAGGCTGAAGTG
Hockemeyer_AAVS1_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
B2
003-051729704R
TTCCCTCCCACCTGGGTCTT
Hockemeyer_AAVS1_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
C1
007-115754830F
CGGTTGAGACTGAAGCATGCAAAG
Hockemeyer_AAVS1_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
C2
007-115754830R
AAGGACCACACTACTGACATTAGATCTACC
Hockemeyer_AAVS1_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
D1
001-024697363F
AGCCTCCCAAAGTGCTAGGAC
Hockemeyer_AAVS1_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
D2
001-024697363R
GTGGAGAACATCCATTCACAGGG
Hockemeyer_AAVS1_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
E1
012-047346370F
CTTGGTATGTTCCAGTGTGGCC
Hockemeyer_AAVS1_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
E2
012-047346370R
GGATGTGTGCAGGATGTACAGG
Hockemeyer_AAVS1_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
F1
00X-031479613F
GTCTGGACATGTCTGAATGCCTAG
Hockemeyer_AAVS1_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
F2
00X-031479613R
GGTTAGGTAAAGTGTCCCCTGC
Hockemeyer_AAVS1_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
G1
009-073729326F
CCACATGCTCAAGAGCCAGG
Hockemeyer_AAVS1_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
G2
009-073729326R
GGCATGTCCCTTAGATTCCAAGGT
Hockemeyer_AAVS1_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
H1
011-057225300F
AGCCTCACCGTACAGATGAGGA
Hockemeyer_AAVS1_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
H2
011-057225300R
TTTAGGTGGCTGGCTGGTGG
Hockemeyer_AAVS1_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
A3
001-202164461F
TGCTCAGTGTTGGGAGTGTCCT
Hockemeyer_AAVS1_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
A4
001-202164461R
TTTGCCAGATGCTGAGCGCC
Hockemeyer_AAVS1_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
B3
005-127879056F
CTCCTGGATAGTGTCAGAGAAAGC
Hockemeyer_AAVS1_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
B4
005-127879056R
CTGCTCCCAATGTAGTTTCCCATG
Hockemeyer_AAVS1_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
C3
017-035084330F
GAGCCTCTGCTGGTTGGATG
Hockemeyer_AAVS1_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
C4
017-035084330R
ATGTTGGGTGCTGGGGAAGG
Hockemeyer_AAVS1_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
D3
008-107822970F
GGCTAAGTGTGAGAATCGAGTGAG
Hockemeyer_AAVS1_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
D4
008-107822970R
GTTAGTGAGACAGCATGTCCCTTC
Hockemeyer_AAVS1_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
E3
006-168932997F
TCCTTCGTCCTGGGATTGGG
Hockemeyer_AAVS1_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
E4
006-168932997R
GGGTGCACAGTTACAGAGGAG
Hockemeyer_AAVS1_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
F3
011-040808649F
CCCATGTCTCAGTCTCTGTTCTAC
Hockemeyer_AAVS1_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
F4
011-040808649R
CTCACTTGTCAGCAAGCACAAAGG
Hockemeyer_AAVS1_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
G3
008-110449611F
GTGCACAACGTGCAGGTTAGTTAC
Hockemeyer_AAVS1_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
G4
008-110449611R
CCTGCAGATTGCAAATACATAATACCCCC
Hockemeyer_AAVS1_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
H3
019-038858026F
TTCCTCTCCCCGCAGACTAC
Hockemeyer_AAVS1_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
H4
019-038858026R
AGTGCACACAGACTGGCCTC
Hockemeyer_AAVS1_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
A5
013-103511881F
TCTCTGGCTGGCAGTTGAGG
Hockemeyer_AAVS1_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
A6
013-103511881R
CAGGTCACTGAAAACTGCAAACCG
Hockemeyer_AAVS1_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
B5
003-124702567F
TGCAAGTCTACAACCAGGGTGTG
Hockemeyer_AAVS1_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
B6
003-124702567R
CAGAAGGCAGCTGGAAGTCTC
Hockemeyer_AAVS1_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
C5
001-201121271F
GAGGGATGAGGGGAAATGGG
Hockemeyer_AAVS1_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
C6
001-201121271R
GGAGCTGTTTGGGTAGATCTATGG
Hockemeyer_AAVS1_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
D5
016-027592598F
CCCTGTGCACATAGGAATAGCC
Hockemeyer_AAVS1_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
D6
016-027592598R
GGGTGATGGGTGCATCAATATCTC
Hockemeyer_AAVS1_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
E5
020-019256133F
GGAAGGGTTTGCAAGCCCTG
Hockemeyer_AAVS1_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
E6
020-019256133R
AAGCAAGCCGCATGCACTAAGG
Hockemeyer_AAVS1_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
F5
011-011075984F
GCCTCATTACTGCTAGGTGGAAG
Hockemeyer_AAVS1_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
F6
011-011075984R
CACCCTAACATCCAGGCATGATGA
Hockemeyer_AAVS1_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
G5
001-178005473F
CTCACAGAAGTGCGAACCGTATTG
Hockemeyer_AAVS1_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
G6
001-178005473R
CACCAATATGGCCACCTCTGTAC
Hockemeyer_AAVS1_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
H5
017-063492561F
CTCTGTTCCCCACTGATCACC
Hockemeyer_AAVS1_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
H6
017-063492561R
AGCATGGAAGGTCCCCCACA
Hockemeyer_AAVS1_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
A7
011-119574363F
CAACGAGGCTGACGCTCTTTC
Hockemeyer_AAVS1_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
A8
011-119574363R
CTGAGCACCTGTGTGCCAGT
Hockemeyer_AAVS1_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
B7
007-069107597F
GATGTCAGTGTCCTCAGCTGTTAG
Hockemeyer_AAVS1_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
B8
007-069107597R
CCTGACTCACAGAAACTGTGGG
Hockemeyer_AAVS1_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
C7
006-011853744F
GTCTACGGATCTAACACTCCAAAAATCTTAGC
Hockemeyer_AAVS1_Ranking-RVDs_5TC	Warning! Primer pair was generated using suboptimal parameters. 
C8
006-011853744R
CTAGGGGGCTGAAATGGAACAAC
Hockemeyer_AAVS1_Ranking-RVDs_5TC	Warning! Primer pair was generated using suboptimal parameters. 
D7
001-206729981F
CTCTCTAGGTTGCCATAACTCTGC
Hockemeyer_AAVS1_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
D8
001-206729981R
TCTCATCAGCCCCGGTGACT
Hockemeyer_AAVS1_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
E7
004-148942680F
GTAGGTAAGCCAGGGAGCTGT
Hockemeyer_AAVS1_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
E8
004-148942680R
CCTGCCAACACCATGTTTCCAC
Hockemeyer_AAVS1_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
F7
011-116565112F
CATCTATGCCCCACCAAGAGG
Hockemeyer_AAVS1_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
F8
011-116565112R
ACATCTGTGGCAGGTGGAGC
Hockemeyer_AAVS1_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
G7
018-070204440F
CCACTCAGGAACACCAGGTTCT
Hockemeyer_AAVS1_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
G8
018-070204440R
CATTGGGAACCTGTCAAGAAGTGC
Hockemeyer_AAVS1_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
H7
012-030180063F
CTATAAGGCTGCTGGGCCTTTC
Hockemeyer_AAVS1_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
H8
012-030180063R
GAGGCAAACCTACTTTATGGAGGG
Hockemeyer_AAVS1_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
A9
008-145493530F
GAGCCATTAAGGGCTAGAGGG
Hockemeyer_AAVS1_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
A10
008-145493530R
GGAAAGCTCCCAAGAAGGGAAG
Hockemeyer_AAVS1_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
B9
008-145324445F
GAGCCATTAAGGGCTAGAGGG
Hockemeyer_AAVS1_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
B10
008-145324445R
GGAAAGCTCCCAAGAAGGGAAG
Hockemeyer_AAVS1_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
C9
001-114585589F
CCCCATAAATGATCTGCATCCAGG
Hockemeyer_AAVS1_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
C10
001-114585589R
CGCTGAGAAGGCCAGATGAG
Hockemeyer_AAVS1_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
D9
020-058618528F
GGGAGCTATGATCATGCCATAGG
Hockemeyer_AAVS1_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
D10
020-058618528R
GCTCCATGTCATTGTTCTGGGC
Hockemeyer_AAVS1_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
E9
001-062633493F
TCCTCCTGCCTAATGGCTTTACTG
Hockemeyer_AAVS1_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
E10
001-062633493R
GCTACTGAGCCCAGCCTAATTTAC
Hockemeyer_AAVS1_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
F9
019-019017199F
GGGATGGGAGCAGATCCACA
Hockemeyer_AAVS1_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
F10
019-019017199R
AAGCCTTCTGTGTCTCGGCC
Hockemeyer_AAVS1_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
G9
007-031005868F
GTCAAAGTCTCTACTCTCTGACCC
Hockemeyer_AAVS1_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
G10
007-031005868R
GGGAGAAGAGCATCTGGAACAGAA
Hockemeyer_AAVS1_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
H9
002-064716657F
GAAATGCATCCCCTCTAGCCTG
Hockemeyer_AAVS1_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
H10
002-064716657R
GCTGCATGGACAGTGCTCACA
Hockemeyer_AAVS1_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
A11
00X-040907277F
CTGGTTAGGAGGGTTGAAGGTG
Hockemeyer_AAVS1_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
A12
00X-040907277R
GGTCAGAGAAGGCTGAGTAGG
Hockemeyer_AAVS1_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
B11
020-031067101F
CTGCTCAGAGACCCAAGGTG
Hockemeyer_AAVS1_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
B12
020-031067101R
TAGAGACAGCGTCTCACTGTGTTG
Hockemeyer_AAVS1_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
C11
021-036773953F
CCAGAGCGGTTTTCCAAGATGC
Hockemeyer_AAVS1_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
C12
021-036773953R
GGATTCACAACACCATGGATGGAC
Hockemeyer_AAVS1_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
D11
007-127776243F
ATGACAGAAGGGGCGTGGTG
Hockemeyer_AAVS1_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
D12
007-127776243R
AGTGGCGCCTCCTAGAAGCA
Hockemeyer_AAVS1_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
E11
010-050821729F
GTGGGGGATGCTTTTGTCTGAG
Hockemeyer_AAVS1_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
E12
010-050821729R
AAGTCTCTGCTCGCTGCCCT
Hockemeyer_AAVS1_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
F11
012-025903897F
CCAGCATCTTGTGGCTGAGTAG
Hockemeyer_AAVS1_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
F12
012-025903897R
GGGAAGATGACAGTGTTCTTGACAGG
Hockemeyer_AAVS1_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
G11
005-157580667F
GTAATCCAGGGTCTCTGTTTGTCC
Hockemeyer_AAVS1_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
G12
005-157580667R
GTCTTTGGGTGGCATTTGTGAGG
Hockemeyer_AAVS1_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
H11
007-030310150F
CTGCTGCCAAAAGCTACCCATG
Hockemeyer_AAVS1_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
H12
007-030310150R
GAGCTCCAGTTTCCTGCACTGT
Hockemeyer_AAVS1_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
Primer Plate #2
A1
007-123033818F
CTACCCCTATGCAGGTTTGCTGA
Hockemeyer_AAVS1_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
A2
007-123033818R
CTTCCCCCAACTCTCACAGG
Hockemeyer_AAVS1_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
B1
006-157508829F
GCCAGCCTCCTCTATTAATGGAGT
Hockemeyer_AAVS1_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
B2
006-157508829R
GAGTAAAGGAAGAAAGCCGTCTTTTGCAG
Hockemeyer_AAVS1_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
C1
012-120471484F
GCCGGCTGAGAGTTCTTGCT
Hockemeyer_AAVS1_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
C2
012-120471484R
GTATGCCTGTATCTGGCTGTGTTG
Hockemeyer_AAVS1_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
D1
015-066339107F
CACACAGTAGGCTTTGCTGGC
Hockemeyer_AAVS1_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
D2
015-066339107R
GGTGAACTTCATGGGATGTGCTG
Hockemeyer_AAVS1_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
E1
017-008242168F
CAGCTATTGCGGGGAGCTAATG
Hockemeyer_AAVS1_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
E2
017-008242168R
AGGCCACAGCTCAGAACTGTGGA
Hockemeyer_AAVS1_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
F1
015-067305574F
CCCCACCTTGCTGAATGGAC
Hockemeyer_AAVS1_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
F2
015-067305574R
CCAGTCCTTGATCTCTTCCTGAGT
Hockemeyer_AAVS1_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
G1
010-035501885F
CAGGATGCTGTGTCCCCTCA
Hockemeyer_AAVS1_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
G2
010-035501885R
GAGCTCCGACTCTGAAGGAAG
Hockemeyer_AAVS1_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
H1
007-140379038F
TCTGCGCCCCTTTGTCACCA
Hockemeyer_AAVS1_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
H2
007-140379038R
CCTCTAAGGCAGGTCACCAAC
Hockemeyer_AAVS1_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
A3
006-092651079F
CAGCAAGGAGGCCAAGAACTCA
Hockemeyer_AAVS1_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
A4
006-092651079R
GTGTGTGGGCCTCAAGTTTTGTAC
Hockemeyer_AAVS1_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
B3
003-070995805F
GCCGTTGCTTTTGCCTTGAACATC
Hockemeyer_AAVS1_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
B4
003-070995805R
TACTTGAGTCCCTGGATCTGGC
Hockemeyer_AAVS1_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
C3
002-001827851F
GGGGGTTGGGGAACGTTTTTAG
Hockemeyer_AAVS1_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
C4
002-001827851R
TCAGGACGAGAGCAGCTCAAATG
Hockemeyer_AAVS1_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
D3
010-024751913F
GGTGGATGTGCAGAGATGGAG
Hockemeyer_AAVS1_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
D4
010-024751913R
CTCTACTGAGCCCTCCAGTG
Hockemeyer_AAVS1_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
E3
004-090656661F
CAGGGGAGTGTGTACATAGTGTAG
Hockemeyer_AAVS1_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
E4
004-090656661R
CCAGAGTTGTTCCTCTCCACATAG
Hockemeyer_AAVS1_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
F3
006-021174562F
GTCGGTATAATCTTAGTCAAAAGTTCAACAGGG
Hockemeyer_AAVS1_Ranking-RVDs_5TC	Warning! Primer pair was generated using suboptimal parameters. 
F4
006-021174562R
TAGTTAAATTGCCCTCCAAAATGGCCTTATCAATG
Hockemeyer_AAVS1_Ranking-RVDs_5TC	Warning! Primer pair was generated using suboptimal parameters. 
G3
001-196973362F
GCAGAAGAGTGTGTCTGCAATTCATTTTCAGT
Hockemeyer_AAVS1_Ranking-RVDs_5TC	Warning! Primer pair was generated using suboptimal parameters. 
G4
001-196973362R
CACAATATGCAGTAGACTCTGGAAAACAGC
Hockemeyer_AAVS1_Ranking-RVDs_5TC	Warning! Primer pair was generated using suboptimal parameters. 
H3
001-029537012F
TGCACCAAATGCTTAGTGCAGGG
Hockemeyer_AAVS1_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
H4
001-029537012R
GTCACACACTAGTGAGTGGTAGAG
Hockemeyer_AAVS1_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
A5
001-163803301F
GGTTAAGTGCTTGACCACTGCTG
Hockemeyer_AAVS1_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
A6
001-163803301R
CACCATGGCACATGTCTACTTGAG
Hockemeyer_AAVS1_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
B5
020-050029460F
AGAAGCACAGGATGAGGCCG
Hockemeyer_AAVS1_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
B6
020-050029460R
CAGTGGATTGACAGAGGCAGATG
Hockemeyer_AAVS1_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
C5
008-010664694F
GTATCCGTGAGACAAGAACAGGAC
Hockemeyer_AAVS1_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
C6
008-010664694R
CCAGGCACTTGGTAGAACTTTCAG
Hockemeyer_AAVS1_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
D5
007-158192599F
TAGTCCCCAGGCACTTGCAG
Hockemeyer_AAVS1_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
D6
007-158192599R
GCCTCGTAAGTTTAGGCATCCTC
Hockemeyer_AAVS1_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
E5
002-096866458F
CGCCGAAACTGGCCTCAAATTCC
Hockemeyer_AAVS1_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
E6
002-096866458R
CTGCCTAGCAGTTACCTTTGTGG
Hockemeyer_AAVS1_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
F5
018-023479636F
CCTGAGCAACAGAGTGAAACTCC
Hockemeyer_AAVS1_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
F6
018-023479636R
GGGTTTCCCTCAGGCATGTG
Hockemeyer_AAVS1_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
G5
007-030310150F
CTGCTGCCAAAAGCTACCCATG
Hockemeyer_AAVS1_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
G6
007-030310150R
GAGCTCCAGTTTCCTGCACTGT
Hockemeyer_AAVS1_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
H5
005-179890957F
AAATGGACCCTCATCGTGGGGTG
Hockemeyer_AAVS1_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
H6
005-179890957R
AATGCTGGGATTTGTAGCTGGGG
Hockemeyer_AAVS1_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
A7
017-036596557F
CCCAGGCTCTGCTTAGGAAC
Hockemeyer_AAVS1_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
A8
017-036596557R
TTCACTGGGTGCCACCAAGG
Hockemeyer_AAVS1_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
B7
008-123011852F
CCCTGATACCTACCAGCTACTTTG
Hockemeyer_AAVS1_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
B8
008-123011852R
GTCCTGTGTGTCATATGTCTGCTC
Hockemeyer_AAVS1_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
C7
003-039683322F
GGTCTCCAGGGAGCATTATACAG
Hockemeyer_AAVS1_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
C8
003-039683322R
CTAGACACAGAGCCTTCAGTAAGC
Hockemeyer_AAVS1_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
D7
017-046139902F
TGTAACCTGGTTCAGCTCAGTCC
Hockemeyer_AAVS1_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
D8
017-046139902R
ACCGCACCAGGCCTCAAACT
Hockemeyer_AAVS1_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
E7
001-230708668F
GGGCACGCGATTTAACCCTTG
Hockemeyer_AAVS1_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
E8
001-230708668R
CCTCCTAACCAACAGAACAGACC
Hockemeyer_AAVS1_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
F7
012-025903897F
CCAGCATCTTGTGGCTGAGTAG
Hockemeyer_AAVS1_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
F8
012-025903897R
GGGAAGATGACAGTGTTCTTGACAGG
Hockemeyer_AAVS1_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
G7
008-108496180F
GATGGGAAAGATGCCTAGGAAGC
Hockemeyer_AAVS1_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
G8
008-108496180R
GGAAACTGAGGGCCACTAAAGTC
Hockemeyer_AAVS1_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
H7
004-088055877F
TGGGGTGTTTGGATTGGGAGG
Hockemeyer_AAVS1_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
H8
004-088055877R
TCAGCTAGCATTTCAGCTCAGGC
Hockemeyer_AAVS1_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
A9
010-096170810F
GGCATGCCAGAGATAGGACTATTC
Hockemeyer_AAVS1_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
A10
010-096170810R
GTGCTACAGTGGCAACCTTCATG
Hockemeyer_AAVS1_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
B9
011-126543310F
CCAGCAGCTCTGGAAATTGGATC
Hockemeyer_AAVS1_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
B10
011-126543310R
GAGGAAGATCTTACAGACCCAACC
Hockemeyer_AAVS1_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
C9
004-115491385F
CATGTTGCACAGGCTGAGTACTCT
Hockemeyer_AAVS1_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
C10
004-115491385R
CCCATAGCTCTTTGGTCAGGTC
Hockemeyer_AAVS1_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
D9
00X-132539743F
CTGCCATAGACTGGGAAACTGGA
Hockemeyer_AAVS1_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
D10
00X-132539743R
CCAGAACACAGCTGGGAATGTGGA
Hockemeyer_AAVS1_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
E9
011-008291463F
GTTTCCTCGGGCAATGAATGAGC
Hockemeyer_AAVS1_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
E10
011-008291463R
GCACAGTCCTCCTTTCTTCACC
Hockemeyer_AAVS1_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
F9
002-149982161F
GGCATCTTCGAGTACCTTAACTCC
Hockemeyer_AAVS1_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
F10
002-149982161R
CTCTTCTCAAGTTCAAGGGTCAGTTGTAC
Hockemeyer_AAVS1_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
G9
007-052309823F
CCTGAGTCCCTGCTGCATTAATTC
Hockemeyer_AAVS1_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
G10
007-052309823R
ACTCTGTGTCCTCAACGGTGTTC
Hockemeyer_AAVS1_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
H9
010-093286041F
GATTCTGAGCAGGGAGCTTGG
Hockemeyer_AAVS1_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
H10
010-093286041R
ACAGGATGAGCTAGGGCCTGA
Hockemeyer_AAVS1_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
A11
016-081100645F
GCCCCCAAAGTGCATGTTTGGA
Hockemeyer_AAVS1_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
A12
016-081100645R
GAGCTCTCCGATAACATCATGGC
Hockemeyer_AAVS1_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
B11
006-021198081F
CCGTGATTTGAACAGGGTTCGC
Hockemeyer_AAVS1_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
B12
006-021198081R
CTCTCCCCTTTAAATGCCCTTTGC
Hockemeyer_AAVS1_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
C11
007-030310150F
CTGCTGCCAAAAGCTACCCATG
Hockemeyer_AAVS1_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
C12
007-030310150R
GAGCTCCAGTTTCCTGCACTGT
Hockemeyer_AAVS1_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
D11
004-085429094F
GTCTCCCTTGCCAGACTATAACC
Hockemeyer_AAVS1_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
D12
004-085429094R
CATGGCCAAGAGCTGTGCTTTC
Hockemeyer_AAVS1_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
E11
001-198360906F
GGTTTTATAGCCCTTCTCCTGCC
Hockemeyer_AAVS1_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
E12
001-198360906R
GTTGTGGGATTTGAGCTGGTGG
Hockemeyer_AAVS1_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
F11
007-140355184F
CCCACAAGGGCCTTATTTTCAGTC
Hockemeyer_AAVS1_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
F12
007-140355184R
GAGAGCTGATTTCACCCCTCTAG
Hockemeyer_AAVS1_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
G11
00X-013108270F
GCCATTTTGGCAGAGCCACTGA
Hockemeyer_AAVS1_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
G12
00X-013108270R
GCATGTTGTGCACGAAATGACCTC
Hockemeyer_AAVS1_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
H11
002-037018108F
TTTCAGCTGCATCCCGGACC
Hockemeyer_AAVS1_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
H12
002-037018108R
AGGAGATGCGGCCAAGTCAG
Hockemeyer_AAVS1_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
Primer Plate #3
A1
004-089270564F
GTGGTTGGGGCACAGCTTAG
Hockemeyer_AAVS1_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
A2
004-089270564R
CACACCTGCCTCCCATTCTATTC
Hockemeyer_AAVS1_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
B1
017-044029096F
GGACGGACTCAGGAGGCAAT
Hockemeyer_AAVS1_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
B2
017-044029096R
CACACCAAGGCTGTTTTCCTCAGA
Hockemeyer_AAVS1_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
C1
ap1-000838311F
ACCAAGGCTGTTTTCCTCAGACC
Hockemeyer_AAVS1_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
C2
ap1-000838311R
AACCACAGGGACGGACTCAG
Hockemeyer_AAVS1_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
D1
011-076020841F
AAGGAGACTCCCCACATGTTGC
Hockemeyer_AAVS1_Ranking-RVDs_5TC	Primer pair generated using optimal parameters. 
D2
011-076020841R
GGTTACAGCTGCTTCTGCCAC
Hockemeyer_AAVS1_Ranking-RVDs_5TC	Primer pair generated using optimal parameters.