Top Cleavage Sites for Hockemeyer_AAVS1_Ranking-RVDs

PROGNOS Summary for Hockemeyer_AAVS1_Ranking-RVDs_5T

The hg19 genome was searched for the TALEN pair targeting:
5'- TTTTCTGTCACCAATCCT NN..NN ACTGTGGGGTGGAGGGGA -3'

Using these RVDs:
Left:  01NG02NG03NG04HD05NG06NN07NG08HD09NI10HD11HD12NI13NI14NG15HD16HD17NG
Right: 01HD02HD03HD04HD05NG06HD07HD08NI09HD10HD11HD12HD13NI14HD15NI16NN17NG

Up to 5 mismatches were allowed in each nuclease half-site.

Allowed spacing distances were: 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26

Only heterodimers of the nuclease pair were included in the output

157 total sites were located
Mismatch Types Genomic Regions
  0 and 0: 1
  2 and 5: 1
  3 and 5: 4
  4 and 4: 1
  4 and 5: 33
  5 and 5: 117
  Exons: 6
  Promoters: 1
  Introns: 70
  Intergenic: 80


The top 100 sites are ranked below according to a weighting of the RVD base preferences. All sites listed contain flanking 5' thymidines.
For the full output, click here.

This output will be deleted from the server one week after it is generated.  Please save your results.

Primer pairs were designed using the 400 bp of sequence on either side of the cleavage site to melt close to 60 °C

For easy visualization of cleavage on gels:
The smallest fragment will be longer than 130 bp, which is also the minimum separation distance between uncleaved and cleaved products.
The two cleavage products will be separated by a minimum size of 0 bp
To optimize assay conditions, the amplicons have a minimum length of 260 bp, a maximum length of 450 bp, and an optimal length of 270 bp.
Rank: 1	Homology Score: 72	RVD Match Score: 2	Left Half-Site RVD Score: 2.343	Right Half-Site RVD Score: 2.083
	Genomic Region: Intron	Closest Gene: PPP1R12C	Caution! This site scores very closely to the intended nuclease target. The potential for cleavage at this site is of great concern.
Match Type: L15R
	           Left Nuclease
	         TTTTCTGTCACCAATCCT
	5'-GGGGC TTTTCTGTCACCAATCCT      GTCCCTAGTGGCCCC       ACTGTGGGGTGGAGGGGA CAGAT-3'
	3'-CCCCG AAAAGACAGTGGTTAGGA      CAGGGATCACCGGGG       TGACACCCCACCTCCCCT GTCTA-5'
	                                                       TGACACCCCACCTCCCCT
	                                                            Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 019-055627003
Forward Primer: 5'-CCAGGAAATGGGGGTGTGTC-3' T_m: 59.508
Reverse Primer: 5'-GTCCACTTCAGGACAGCATGTTTG-3' T_m: 59.038
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 131, 139
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr19:55627107-55627157

Rank: 2	Homology Score: 17	RVD Match Score: 3.15	Left Half-Site RVD Score: 3.963	Right Half-Site RVD Score: 7.356
	Genomic Region: Intergenic	Closest Gene: TFEC
Match Type: R11L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-CCCCC TCCCCGCCACCCCCCAGT        AATGGGGAGAG         AGGACATTTGACAGAGAA TGAAG-3'
	3'-GGGGG AGGGGCGGTGGGGGGTCA        TTACCCCTCTC         TCCTGTAAACTGTCTCTT ACTTC-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 007-115754830
Forward Primer: 5'-CGGTTGAGACTGAAGCATGCAAAG-3' T_m: 59.038
Reverse Primer: 5'-AAGGACCACACTACTGACATTAGATCTACC-3' T_m: 60.058
Expected PCR Product Length: 274
Expected CelI Cleavage Product Sizes: 143, 131
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr7:115754948-115754994

Rank: 3	Homology Score: 10	RVD Match Score: 3.161	Left Half-Site RVD Score: 3.845	Right Half-Site RVD Score: 7.621
	Genomic Region: Intron	Closest Gene: STPG1
Match Type: R11L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-ACACT TCCCCACCACACCACAAT        GCCCTGTCTCA         AGAAACTGTGACAGAATA AAATG-3'
	3'-TGTGA AGGGGTGGTGTGGTGTTA        CGGGACAGAGT         TCTTTGACACTGTCTTAT TTTAC-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 001-024697363
Forward Primer: 5'-AGCCTCCCAAAGTGCTAGGAC-3' T_m: 59.378
Reverse Primer: 5'-GTGGAGAACATCCATTCACAGGG-3' T_m: 59.148
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 135, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr1:24697473-24697519

Rank: 4	Homology Score: 5	RVD Match Score: 3.195	Left Half-Site RVD Score: 6.316	Right Half-Site RVD Score: 5.03
	Genomic Region: Intron	Closest Gene: DMD
Match Type: L23R
	           Left Nuclease
	         TTTTCTGTCACCAATCCT
	5'-TCTTC TCTTCCATCACTAATTCT  GATGAACAGCTATCAAGCTATTC   ATGGTAGGGTGGAGGGTA GGTGG-3'
	3'-AGAAG AGAAGGTAGTGATTAAGA  CTACTTGTCGATAGTTCGATAAG   TACCATCCCACCTCCCAT CCACC-5'
	                                                       TGACACCCCACCTCCCCT
	                                                            Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 00X-031479613
Forward Primer: 5'-GTCTGGACATGTCTGAATGCCTAG-3' T_m: 59.038
Reverse Primer: 5'-GGTTAGGTAAAGTGTCCCCTGC-3' T_m: 59.258
Expected PCR Product Length: 339
Expected CelI Cleavage Product Sizes: 156, 183
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chrX:31479738-31479796

Rank: 5	Homology Score: 5	RVD Match Score: 3.25	Left Half-Site RVD Score: 6.391	Right Half-Site RVD Score: 5.326
	Genomic Region: Intergenic	Closest Gene: MRM1
Match Type: L25R
	           Left Nuclease
	         TTTTCTGTCACCAATCCT
	5'-TTCTT TTATCTGCCCCCAACACT CCAGGCATTATGTGCATTCATAAAA  AAGGGGGGGTGGAGTGGA GAGAA-3'
	3'-AAGAA AATAGACGGGGGTTGTGA GGTCCGTAATACACGTAAGTATTTT  TTCCCCCCCACCTCACCT CTCTT-5'
	                                                       TGACACCCCACCTCCCCT
	                                                            Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 017-035084330
Forward Primer: 5'-GAGCCTCTGCTGGTTGGATG-3' T_m: 59.508
Reverse Primer: 5'-ATGTTGGGTGCTGGGGAAGG-3' T_m: 59.508
Expected PCR Product Length: 287
Expected CelI Cleavage Product Sizes: 131, 156
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr17:35084429-35084489

Rank: 6	Homology Score: 10	RVD Match Score: 3.291	Left Half-Site RVD Score: 7.204	Right Half-Site RVD Score: 4.928
	Genomic Region: Intron	Closest Gene: ERCC5
Match Type: L20R
	           Left Nuclease
	         TTTTCTGTCACCAATCCT
	5'-CTTTA TTTTCTTTCTGCAACCAT    GAAGTCTCTGGAAGTGGTGG    ACTGTAGGGTGGTGTGGA GTAGC-3'
	3'-GAAAT AAAAGAAAGACGTTGGTA    CTTCAGAGACCTTCACCACC    TGACATCCCACCACACCT CATCG-5'
	                                                       TGACACCCCACCTCCCCT
	                                                            Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 013-103511881
Forward Primer: 5'-TCTCTGGCTGGCAGTTGAGG-3' T_m: 59.508
Reverse Primer: 5'-CAGGTCACTGAAAACTGCAAACCG-3' T_m: 59.038
Expected PCR Product Length: 346
Expected CelI Cleavage Product Sizes: 131, 215
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr13:103511982-103512037

Rank: 7	Homology Score: 5	RVD Match Score: 3.295	Left Half-Site RVD Score: 6.076	Right Half-Site RVD Score: 5.904
	Genomic Region: Intron	Closest Gene: HEG1
Match Type: R18L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-CTCTA TCCCTTCCACCTCACCAA     ATAGTCCTCGCTAGCAGA     AGGGTTGGGTACAGAACA ACAGT-3'
	3'-GAGAT AGGGAAGGTGGAGTGGTT     TATCAGGAGCGATCGTCT     TCCCAACCCATGTCTTGT TGTCA-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 003-124702567
Forward Primer: 5'-TGCAAGTCTACAACCAGGGTGTG-3' T_m: 59.148
Reverse Primer: 5'-CAGAAGGCAGCTGGAAGTCTC-3' T_m: 59.378
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 134, 136
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr3:124702672-124702725

Rank: 8	Homology Score: 5	RVD Match Score: 3.316	Left Half-Site RVD Score: 6.275	Right Half-Site RVD Score: 5.88
	Genomic Region: Intergenic	Closest Gene: LOC729013
Match Type: L25R
	           Left Nuclease
	         TTTTCTGTCACCAATCCT
	5'-AGGTC TCCTCTGACACCACTCCA GTGAGTAGTGGAAAGATGCCTTATT  ACTGCTGGGTGGGGGTGA AGGTC-3'
	3'-TCCAG AGGAGACTGTGGTGAGGT CACTCATCACCTTTCTACGGAATAA  TGACGACCCACCCCCACT TCCAG-5'
	                                                       TGACACCCCACCTCCCCT
	                                                            Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 011-011075984
Forward Primer: 5'-GCCTCATTACTGCTAGGTGGAAG-3' T_m: 59.148
Reverse Primer: 5'-CACCCTAACATCCAGGCATGATGA-3' T_m: 59.038
Expected PCR Product Length: 316
Expected CelI Cleavage Product Sizes: 152, 164
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr11:11076104-11076164

Rank: 9	Homology Score: 5	RVD Match Score: 3.323	Left Half-Site RVD Score: 6.587	Right Half-Site RVD Score: 5.595
	Genomic Region: Intron	Closest Gene: PVRL1
Match Type: R17L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-TAAAG TCCTCTGCCACCCACACT     CTTCATGAGATCCCAAA      AGGATTGATCATGGAAAA TGCAA-3'
	3'-ATTTC AGGAGACGGTGGGTGTGA     GAAGTACTCTAGGGTTT      TCCTAACTAGTACCTTTT ACGTT-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 011-119574363
Forward Primer: 5'-CAACGAGGCTGACGCTCTTTC-3' T_m: 59.378
Reverse Primer: 5'-CTGAGCACCTGTGTGCCAGT-3' T_m: 59.508
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 135, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr11:119574470-119574522

Rank: 10	Homology Score: 2	RVD Match Score: 3.331	Left Half-Site RVD Score: 6.028	Right Half-Site RVD Score: 6.229
	Genomic Region: Intergenic	Closest Gene: ADTRP
Match Type: R14L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-TAGCT TCCCTCCCACCCCCCACC       CTGGAACAACAGAT       TGGGTTAGTGCTAGAAAA TGTCT-3'
	3'-ATCGA AGGGAGGGTGGGGGGTGG       GACCTTGTTGTCTA       ACCCAATCACGATCTTTT ACAGA-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Warning! Primer pair was generated using suboptimal parameters. 
Primer Index: 006-011853744
Forward Primer: 5'-GTCTACGGATCTAACACTCCAAAAATCTTAGC-3' T_m: 59.848
Reverse Primer: 5'-CTAGGGGGCTGAAATGGAACAAC-3' T_m: 59.148
Expected PCR Product Length: 325
Expected CelI Cleavage Product Sizes: 172, 153
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr6:11853889-11853938

Rank: 11	Homology Score: 5	RVD Match Score: 3.335	Left Half-Site RVD Score: 4.892	Right Half-Site RVD Score: 7.621
	Genomic Region: Intron	Closest Gene: ARHGAP10
Match Type: R17L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-TCCTG TTCCCACCACCCCACAAC     CTTCTTACATCTGAACA      ATGACAGATGACAGTAAA TCCAG-3'
	3'-AGGAC AAGGGTGGTGGGGTGTTG     GAAGAATGTAGACTTGT      TACTGTCTACTGTCATTT AGGTC-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 004-148942680
Forward Primer: 5'-GTAGGTAAGCCAGGGAGCTGT-3' T_m: 59.378
Reverse Primer: 5'-CCTGCCAACACCATGTTTCCAC-3' T_m: 59.258
Expected PCR Product Length: 299
Expected CelI Cleavage Product Sizes: 158, 141
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr4:148942810-148942862

Rank: 12	Homology Score: 5	RVD Match Score: 3.351	Left Half-Site RVD Score: 6.125	Right Half-Site RVD Score: 6.27
	Genomic Region: Intergenic	Closest Gene: TMTC1
Match Type: L12R
	           Left Nuclease
	         TTTTCTGTCACCAATCCT
	5'-GATTA TTTTCTAACAACCTTCCT        ACTGAGGAACAT        ACTCTGGGATGGATTGGA AGTGC-3'
	3'-CTAAT AAAAGATTGTTGGAAGGA        TGACTCCTTGTA        TGAGACCCTACCTAACCT TCACG-5'
	                                                       TGACACCCCACCTCCCCT
	                                                            Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 012-030180063
Forward Primer: 5'-CTATAAGGCTGCTGGGCCTTTC-3' T_m: 59.258
Reverse Primer: 5'-GAGGCAAACCTACTTTATGGAGGG-3' T_m: 59.038
Expected PCR Product Length: 333
Expected CelI Cleavage Product Sizes: 131, 202
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr12:30180168-30180215

Rank: 13	Homology Score: 5	RVD Match Score: 3.352	Left Half-Site RVD Score: 5.506	Right Half-Site RVD Score: 6.897
	Genomic Region: Intron	Closest Gene: BOP1
Match Type: L22R
	           Left Nuclease
	         TTTTCTGTCACCAATCCT
	5'-CCAAA TTTCCTCTCCCTAATCCT   GCAGGAGCCCACAGCTGGCCAG   AGGGAGGGGAGGAGAGGA CAAGC-3'
	3'-GGTTT AAAGGAGAGGGATTAGGA   CGTCCTCGGGTGTCGACCGGTC   TCCCTCCCCTCCTCTCCT GTTCG-5'
	                                                       TGACACCCCACCTCCCCT
	                                                            Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 008-145493530
Forward Primer: 5'-GAGCCATTAAGGGCTAGAGGG-3' T_m: 59.378
Reverse Primer: 5'-GGAAAGCTCCCAAGAAGGGAAG-3' T_m: 59.258
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 135, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr8:145493634-145493691

Rank: 14	Homology Score: 5	RVD Match Score: 3.352	Left Half-Site RVD Score: 5.506	Right Half-Site RVD Score: 6.897
	Genomic Region: Intergenic	Closest Gene: SCXB
Match Type: L22R
	           Left Nuclease
	         TTTTCTGTCACCAATCCT
	5'-CCAAA TTTCCTCTCCCTAATCCT   GCAGGAGCCCACAGCTGGCCAG   AGGGAGGGGAGGAGAGGA CAAGC-3'
	3'-GGTTT AAAGGAGAGGGATTAGGA   CGTCCTCGGGTGTCGACCGGTC   TCCCTCCCCTCCTCTCCT GTTCG-5'
	                                                       TGACACCCCACCTCCCCT
	                                                            Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 008-145324445
Forward Primer: 5'-GAGCCATTAAGGGCTAGAGGG-3' T_m: 59.378
Reverse Primer: 5'-GGAAAGCTCCCAAGAAGGGAAG-3' T_m: 59.258
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 135, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr8:145324549-145324606

Rank: 15	Homology Score: 5	RVD Match Score: 3.364	Left Half-Site RVD Score: 5.827	Right Half-Site RVD Score: 6.708
	Genomic Region: Intergenic	Closest Gene: C20orf197
Match Type: R16L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-GCACC TCCCATCAACCCCATAGC      AGGAATGGGGCGAACT      TTGATTGATGTAAGAAAA TGAAA-3'
	3'-CGTGG AGGGTAGTTGGGGTATCG      TCCTTACCCCGCTTGA      AACTAACTACATTCTTTT ACTTT-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 020-058618528
Forward Primer: 5'-GGGAGCTATGATCATGCCATAGG-3' T_m: 59.148
Reverse Primer: 5'-GCTCCATGTCATTGTTCTGGGC-3' T_m: 59.258
Expected PCR Product Length: 290
Expected CelI Cleavage Product Sizes: 135, 155
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr20:58618635-58618686

Rank: 16	Homology Score: 8	RVD Match Score: 3.364	Left Half-Site RVD Score: 6.153	Right Half-Site RVD Score: 6.349
	Genomic Region: Intergenic	Closest Gene: INADL
Match Type: R25L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-TCAAC TCCTCTCAACCCCAGAGC CATGTGTTACTCTTTGGGGAACTTC  AGGATAGCTGTCTGAAAA CAGTC-3'
	3'-AGTTG AGGAGAGTTGGGGTCTCG GTACACAATGAGAAACCCCTTGAAG  TCCTATCGACAGACTTTT GTCAG-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 001-062633493
Forward Primer: 5'-TCCTCCTGCCTAATGGCTTTACTG-3' T_m: 59.038
Reverse Primer: 5'-GCTACTGAGCCCAGCCTAATTTAC-3' T_m: 59.038
Expected PCR Product Length: 273
Expected CelI Cleavage Product Sizes: 130, 143
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr1:62633591-62633651

Rank: 17	Homology Score: 2	RVD Match Score: 3.368	Left Half-Site RVD Score: 6.586	Right Half-Site RVD Score: 5.927
	Genomic Region: Intergenic	Closest Gene: LGALSL
Match Type: R26L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-CTGCC TCCCCTCCCCACAATATT TACAAGCATTGATTTTCTAGAAGGGA AGGGGTGGGGAGGGAAAA AGGAG-3'
	3'-GACGG AGGGGAGGGGTGTTATAA ATGTTCGTAACTAAAAGATCTTCCCT TCCCCACCCCTCCCTTTT TCCTC-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 002-064716657
Forward Primer: 5'-GAAATGCATCCCCTCTAGCCTG-3' T_m: 59.258
Reverse Primer: 5'-GCTGCATGGACAGTGCTCACA-3' T_m: 59.378
Expected PCR Product Length: 315
Expected CelI Cleavage Product Sizes: 131, 184
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr2:64716755-64716816

Rank: 18	Homology Score: 5	RVD Match Score: 3.389	Left Half-Site RVD Score: 6.267	Right Half-Site RVD Score: 6.409
	Genomic Region: Intergenic	Closest Gene: MIR802
Match Type: L21R
	           Left Nuclease
	         TTTTCTGTCACCAATCCT
	5'-CAGAG TCCTTTCCCACCAATCCT   TCATCAGCCTAATCAATACTA    GCAGTGGGGTGGAAGGAA GATGA-3'
	3'-GTCTC AGGAAAGGGTGGTTAGGA   AGTAGTCGGATTAGTTATGAT    CGTCACCCCACCTTCCTT CTACT-5'
	                                                       TGACACCCCACCTCCCCT
	                                                            Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 021-036773953
Forward Primer: 5'-CCAGAGCGGTTTTCCAAGATGC-3' T_m: 59.258
Reverse Primer: 5'-GGATTCACAACACCATGGATGGAC-3' T_m: 59.038
Expected PCR Product Length: 371
Expected CelI Cleavage Product Sizes: 175, 196
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr21:36774098-36774154

Rank: 19	Homology Score: 5	RVD Match Score: 3.396	Left Half-Site RVD Score: 5.513	Right Half-Site RVD Score: 7.23
	Genomic Region: Intergenic	Closest Gene: CLINT1
Match Type: L15R
	           Left Nuclease
	         TTTTCTGTCACCAATCCT
	5'-TGCAC TTTTCAGCCCCCACTCCT      CCTTTATGAAGAGAA       CCTGTGTGCTGGTGGGAA GAGCT-3'
	3'-ACGTG AAAAGTCGGGGGTGAGGA      GGAAATACTTCTCTT       GGACACACGACCACCCTT CTCGA-5'
	                                                       TGACACCCCACCTCCCCT
	                                                            Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 005-157580667
Forward Primer: 5'-GTAATCCAGGGTCTCTGTTTGTCC-3' T_m: 59.038
Reverse Primer: 5'-GTCTTTGGGTGGCATTTGTGAGG-3' T_m: 59.148
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 133, 137
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr5:157580773-157580823

Rank: 20	Homology Score: 2	RVD Match Score: 3.41	Left Half-Site RVD Score: 5.997	Right Half-Site RVD Score: 6.886
	Genomic Region: Promoter	Closest Gene: ODF4
Match Type: R20L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-TCACC TCCCATCACCACCACACT    GGGGAGCAAATTTCAGCATG    AGGTTTGGTGAAGACAAA TTAAC-3'
	3'-AGTGG AGGGTAGTGGTGGTGTGA    CCCCTCGTTTAAAGTCGTAC    TCCAAACCACTTCTGTTT AATTG-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 017-008242168
Forward Primer: 5'-CAGCTATTGCGGGGAGCTAATG-3' T_m: 59.258
Reverse Primer: 5'-AGGCCACAGCTCAGAACTGTGGA-3' T_m: 61.088
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 136, 134
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr17:8242274-8242329

Rank: 21	Homology Score: 5	RVD Match Score: 3.411	Left Half-Site RVD Score: 7.369	Right Half-Site RVD Score: 5.492
	Genomic Region: Intergenic	Closest Gene: SMAD3
Match Type: R13L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-TGTTC TTCCACCCAGCCCTCAGT       GCTCTGCTGAGGC        AGGATGGGTGGCTGGAAA AATGG-3'
	3'-ACAAG AAGGTGGGTCGGGAGTCA       CGAGACGACTCCG        TCCTACCCACCGACCTTT TTACC-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 015-067305574
Forward Primer: 5'-CCCCACCTTGCTGAATGGAC-3' T_m: 59.508
Reverse Primer: 5'-CCAGTCCTTGATCTCTTCCTGAGT-3' T_m: 59.038
Expected PCR Product Length: 275
Expected CelI Cleavage Product Sizes: 142, 133
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr15:67305690-67305738

Rank: 22	Homology Score: 2	RVD Match Score: 3.412	Left Half-Site RVD Score: 6.17	Right Half-Site RVD Score: 6.707
	Genomic Region: Intergenic	Closest Gene: MIR4643
Match Type: R11L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-GACCC TCCGCACCACCCCTCAAC        ACCTCTCCCAT         ATGAGGGGTGACAGCTAA TTGTT-3'
	3'-CTGGG AGGCGTGGTGGGGAGTTG        TGGAGAGGGTA         TACTCCCCACTGTCGATT AACAA-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 006-092651079
Forward Primer: 5'-CAGCAAGGAGGCCAAGAACTCA-3' T_m: 59.258
Reverse Primer: 5'-GTGTGTGGGCCTCAAGTTTTGTAC-3' T_m: 59.038
Expected PCR Product Length: 352
Expected CelI Cleavage Product Sizes: 151, 201
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr6:92651205-92651251

Rank: 23	Homology Score: 10	RVD Match Score: 3.414	Left Half-Site RVD Score: 5.315	Right Half-Site RVD Score: 7.738
	Genomic Region: Intergenic	Closest Gene: FOXP1
Match Type: R20L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-TGAAA TGCCCCCCACCCCAGAGT    ATATTCATTAGGACTCTTTG    ATTATAAGAGACAGAAAA TCAAC-3'
	3'-ACTTT ACGGGGGGTGGGGTCTCA    TATAAGTAATCCTGAGAAAC    TAATATTCTCTGTCTTTT AGTTG-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 003-070995805
Forward Primer: 5'-GCCGTTGCTTTTGCCTTGAACATC-3' T_m: 59.038
Reverse Primer: 5'-TACTTGAGTCCCTGGATCTGGC-3' T_m: 59.258
Expected PCR Product Length: 306
Expected CelI Cleavage Product Sizes: 176, 130
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr3:70995951-70996006

Rank: 24	Homology Score: 10	RVD Match Score: 3.415	Left Half-Site RVD Score: 5.371	Right Half-Site RVD Score: 7.681
	Genomic Region: Intron	Closest Gene: MYT1L
Match Type: R15L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-GAATG TTCCCTCCAACCCAGAGT      GCTGAGAAGGCTGGC       AGGATAGAGGCCAGAACA TGTCT-3'
	3'-CTTAC AAGGGAGGTTGGGTCTCA      CGACTCTTCCGACCG       TCCTATCTCCGGTCTTGT ACAGA-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 002-001827851
Forward Primer: 5'-GGGGGTTGGGGAACGTTTTTAG-3' T_m: 59.258
Reverse Primer: 5'-TCAGGACGAGAGCAGCTCAAATG-3' T_m: 59.148
Expected PCR Product Length: 446
Expected CelI Cleavage Product Sizes: 315, 131
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr2:1828139-1828189

Rank: 25	Homology Score: 5	RVD Match Score: 3.418	Left Half-Site RVD Score: 6.211	Right Half-Site RVD Score: 6.677
	Genomic Region: Intron	Closest Gene: KIAA1217
Match Type: L20R
	           Left Nuclease
	         TTTTCTGTCACCAATCCT
	5'-GACAT TTTTCTTTTATCCATCCT    ACAGCACTGCACAATTATTG    ATTGTGGCTTGGAAGCGA AATGA-3'
	3'-CTGTA AAAAGAAAATAGGTAGGA    TGTCGTGACGTGTTAATAAC    TAACACCGAACCTTCGCT TTACT-5'
	                                                       TGACACCCCACCTCCCCT
	                                                            Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 010-024751913
Forward Primer: 5'-GGTGGATGTGCAGAGATGGAG-3' T_m: 59.378
Reverse Primer: 5'-CTCTACTGAGCCCTCCAGTG-3' T_m: 59.508
Expected PCR Product Length: 343
Expected CelI Cleavage Product Sizes: 208, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr10:24752091-24752146

Rank: 26	Homology Score: 2	RVD Match Score: 3.42	Left Half-Site RVD Score: 6.697	Right Half-Site RVD Score: 6.234
	Genomic Region: Intron	Closest Gene: SNCA
Match Type: L18R
	           Left Nuclease
	         TTTTCTGTCACCAATCCT
	5'-TTCAA TTTTCTATTATCAATTCC     TAGTCTGCTCATAATGCC     ATTTTGGGGTGGGGAGCA AAGGG-3'
	3'-AAGTT AAAAGATAATAGTTAAGG     ATCAGACGAGTATTACGG     TAAAACCCCACCCCTCGT TTCCC-5'
	                                                       TGACACCCCACCTCCCCT
	                                                            Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 004-090656661
Forward Primer: 5'-CAGGGGAGTGTGTACATAGTGTAG-3' T_m: 59.038
Reverse Primer: 5'-CCAGAGTTGTTCCTCTCCACATAG-3' T_m: 59.038
Expected PCR Product Length: 359
Expected CelI Cleavage Product Sizes: 169, 190
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr4:90656801-90656854

Rank: 27	Homology Score: 2	RVD Match Score: 3.426	Left Half-Site RVD Score: 6.986	Right Half-Site RVD Score: 6.025
	Genomic Region: Intron	Closest Gene: CDKAL1
Match Type: L17R
	           Left Nuclease
	         TTTTCTGTCACCAATCCT
	5'-TCCCC TTTTATGTCAACAATTAA     AAAATGTTATATACAGT      ATTGTTGGGTGTAGGTAA GAAAA-3'
	3'-AGGGG AAAATACAGTTGTTAATT     TTTTACAATATATGTCA      TAACAACCCACATCCATT CTTTT-5'
	                                                       TGACACCCCACCTCCCCT
	                                                            Right Nuclease
Warning! Primer pair was generated using suboptimal parameters. 
Primer Index: 006-021174562
Forward Primer: 5'-GTCGGTATAATCTTAGTCAAAAGTTCAACAGGG-3' T_m: 59.758
Reverse Primer: 5'-TAGTTAAATTGCCCTCCAAAATGGCCTTATCAATG-3' T_m: 59.578
Expected PCR Product Length: 406
Expected CelI Cleavage Product Sizes: 275, 131
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr6:21174809-21174861

Rank: 28	Homology Score: 5	RVD Match Score: 3.445	Left Half-Site RVD Score: 5.52	Right Half-Site RVD Score: 7.746
	Genomic Region: Intergenic	Closest Gene: NUF2
Match Type: R11L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-TTGCT TGACCTCCACACCACACT        CCATCTCTCTT         GGGATAAGTGCCAAAAAA GAGGT-3'
	3'-AACGA ACTGGAGGTGTGGTGTGA        GGTAGAGAGAA         CCCTATTCACGGTTTTTT CTCCA-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 001-163803301
Forward Primer: 5'-GGTTAAGTGCTTGACCACTGCTG-3' T_m: 59.148
Reverse Primer: 5'-CACCATGGCACATGTCTACTTGAG-3' T_m: 59.038
Expected PCR Product Length: 336
Expected CelI Cleavage Product Sizes: 139, 197
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr1:163803415-163803461

Rank: 29	Homology Score: 5	RVD Match Score: 3.445	Left Half-Site RVD Score: 6.803	Right Half-Site RVD Score: 6.322
	Genomic Region: Intron	Closest Gene: NFATC2
Match Type: L25R
	           Left Nuclease
	         TTTTCTGTCACCAATCCT
	5'-TCGGG TTTGCTGTCACAACAACT GCAGGATGACCCGGTGCTGGGCAGC  ACTGCTGGCTGGAGGTGA GAAAT-3'
	3'-AGCCC AAACGACAGTGTTGTTGA CGTCCTACTGGGCCACGACCCGTCG  TGACGACCGACCTCCACT CTTTA-5'
	                                                       TGACACCCCACCTCCCCT
	                                                            Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 020-050029460
Forward Primer: 5'-AGAAGCACAGGATGAGGCCG-3' T_m: 59.508
Reverse Primer: 5'-CAGTGGATTGACAGAGGCAGATG-3' T_m: 59.148
Expected PCR Product Length: 336
Expected CelI Cleavage Product Sizes: 205, 131
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr20:50029633-50029693

Rank: 30	Homology Score: 5	RVD Match Score: 3.449	Left Half-Site RVD Score: 7.012	Right Half-Site RVD Score: 6.165
	Genomic Region: Intron	Closest Gene: PTPRN2
Match Type: L25R
	           Left Nuclease
	         TTTTCTGTCACCAATCCT
	5'-AGAGA TCTGCTGTGACCACACCT GCAGGGTACTCAGGCCACCTTTCAT  ACTGGGGTGAGGAGAGGA AGTAA-3'
	3'-TCTCT AGACGACACTGGTGTGGA CGTCCCATGAGTCCGGTGGAAAGTA  TGACCCCACTCCTCTCCT TCATT-5'
	                                                       TGACACCCCACCTCCCCT
	                                                            Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 007-158192599
Forward Primer: 5'-TAGTCCCCAGGCACTTGCAG-3' T_m: 59.508
Reverse Primer: 5'-GCCTCGTAAGTTTAGGCATCCTC-3' T_m: 59.148
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 137, 133
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr7:158192704-158192764

Rank: 31	Homology Score: 5	RVD Match Score: 3.455	Left Half-Site RVD Score: 5.689	Right Half-Site RVD Score: 7.621
	Genomic Region: Intron	Closest Gene: STARD7
Match Type: R17L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-CTCCG TGCCCTCCACCTCACCCT     ACCCCCTGCAACATTTA      AAGAAAGGTTACAGATAA GAAAC-3'
	3'-GAGGC ACGGGAGGTGGAGTGGGA     TGGGGGACGTTGTAAAT      TTCTTTCCAATGTCTATT CTTTG-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 002-096866458
Forward Primer: 5'-CGCCGAAACTGGCCTCAAATTCC-3' T_m: 61.088
Reverse Primer: 5'-CTGCCTAGCAGTTACCTTTGTGG-3' T_m: 59.148
Expected PCR Product Length: 295
Expected CelI Cleavage Product Sizes: 130, 165
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr2:96866560-96866612

Rank: 32	Homology Score: 2	RVD Match Score: 3.459	Left Half-Site RVD Score: 7.07	Right Half-Site RVD Score: 6.185
	Genomic Region: Intergenic	Closest Gene: ZNRF2
Match Type: L14R
	           Left Nuclease
	         TTTTCTGTCACCAATCCT
	5'-TCCCA TCCTCTGTCATCTATCCC       TACAATCTGCAGCC       TGTGTGGTGTGGGGGGCA GGAGA-3'
	3'-AGGGT AGGAGACAGTAGATAGGG       ATGTTAGACGTCGG       ACACACCACACCCCCCGT CCTCT-5'
	                                                       TGACACCCCACCTCCCCT
	                                                            Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 007-030310147
Forward Primer: 5'-TTGCTGCTGCCAAAAGCTACCC-3' T_m: 59.258
Reverse Primer: 5'-GAGCTCCAGTTTCCTGCACTGT-3' T_m: 59.258
Expected PCR Product Length: 389
Expected CelI Cleavage Product Sizes: 131, 258
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr7:30310251-30310300

Rank: 33	Homology Score: 5	RVD Match Score: 3.472	Left Half-Site RVD Score: 6.794	Right Half-Site RVD Score: 6.526
	Genomic Region: Intergenic	Closest Gene: MOBP
Match Type: L13R
	           Left Nuclease
	         TTTTCTGTCACCAATCCT
	5'-TAGAT TTTTTTGTAAGCAATCTT       TTGAGACACAAGA        AGTGGGGTGTAGAGAGGA ATTCA-3'
	3'-ATCTA AAAAAACATTCGTTAGAA       AACTCTGTGTTCT        TCACCCCACATCTCTCCT TAAGT-5'
	                                                       TGACACCCCACCTCCCCT
	                                                            Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 003-039683322
Forward Primer: 5'-GGTCTCCAGGGAGCATTATACAG-3' T_m: 59.148
Reverse Primer: 5'-CTAGACACAGAGCCTTCAGTAAGC-3' T_m: 59.038
Expected PCR Product Length: 410
Expected CelI Cleavage Product Sizes: 229, 181
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr3:39683525-39683573

Rank: 34	Homology Score: 2	RVD Match Score: 3.472	Left Half-Site RVD Score: 6.894	Right Half-Site RVD Score: 6.408
	Genomic Region: Intergenic	Closest Gene: NFE2L1
Match Type: R12L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-GAGCC TCTCCACAACTCCCCAGT        CTCCAGTTGGAA        AGGTTTGGTCATATCAAA CAAGA-3'
	3'-CTCGG AGAGGTGTTGAGGGGTCA        GAGGTCAACCTT        TCCAAACCAGTATAGTTT GTTCT-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 017-046139902
Forward Primer: 5'-TGTAACCTGGTTCAGCTCAGTCC-3' T_m: 59.148
Reverse Primer: 5'-ACCGCACCAGGCCTCAAACT-3' T_m: 59.508
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 135, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr17:46140011-46140058

Rank: 35	Homology Score: 2	RVD Match Score: 3.477	Left Half-Site RVD Score: 6.501	Right Half-Site RVD Score: 6.839
	Genomic Region: Intergenic	Closest Gene: IFLTD1
Match Type: L22R
	           Left Nuclease
	         TTTTCTGTCACCAATCCT
	5'-CCAAC TTTTCTCACACAAATTAT   CTCATTTAGTTTTCCGAATAAC   TCTCTGGGGGAGGGGGGA ATTAA-3'
	3'-GGTTG AAAAGAGTGTGTTTAATA   GAGTAAATCAAAAGGCTTATTG   AGAGACCCCCTCCCCCCT TAATT-5'
	                                                       TGACACCCCACCTCCCCT
	                                                            Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 012-025903897
Forward Primer: 5'-CCAGCATCTTGTGGCTGAGTAG-3' T_m: 59.258
Reverse Primer: 5'-GGGAAGATGACAGTGTTCTTGACAGG-3' T_m: 60.578
Expected PCR Product Length: 290
Expected CelI Cleavage Product Sizes: 131, 159
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr12:25903997-25904054

Rank: 36	Homology Score: 2	RVD Match Score: 3.482	Left Half-Site RVD Score: 6.572	Right Half-Site RVD Score: 6.825
	Genomic Region: Intron	Closest Gene: ANGPT1
Match Type: R12L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-ATCTC TTCCCTGCTCCCCACCAT        TCAGAATTTGTT        GTAATTTGTGAAAGAAAA GTGAA-3'
	3'-TAGAG AAGGGACGAGGGGTGGTA        AGTCTTAAACAA        CATTAAACACTTTCTTTT CACTT-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 008-108496180
Forward Primer: 5'-GATGGGAAAGATGCCTAGGAAGC-3' T_m: 59.148
Reverse Primer: 5'-GGAAACTGAGGGCCACTAAAGTC-3' T_m: 59.148
Expected PCR Product Length: 339
Expected CelI Cleavage Product Sizes: 186, 153
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr8:108496340-108496387

Rank: 37	Homology Score: 5	RVD Match Score: 3.483	Left Half-Site RVD Score: 6.805	Right Half-Site RVD Score: 6.595
	Genomic Region: Intron	Closest Gene: AFF1
Match Type: L21R
	           Left Nuclease
	         TTTTCTGTCACCAATCCT
	5'-TTCTC TATTCTGCCTCAAAACCT   AGTTGCGGTTGTTCCTTGAGC    ACTGTGGCATGGAGCTGA GTTTC-3'
	3'-AAGAG ATAAGACGGAGTTTTGGA   TCAACGCCAACAAGGAACTCG    TGACACCGTACCTCGACT CAAAG-5'
	                                                       TGACACCCCACCTCCCCT
	                                                            Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 004-088055877
Forward Primer: 5'-TGGGGTGTTTGGATTGGGAGG-3' T_m: 59.378
Reverse Primer: 5'-TCAGCTAGCATTTCAGCTCAGGC-3' T_m: 59.148
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 135, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr4:88055982-88056038

Rank: 38	Homology Score: 5	RVD Match Score: 3.484	Left Half-Site RVD Score: 6.521	Right Half-Site RVD Score: 6.873
	Genomic Region: Intron	Closest Gene: TBC1D12
Match Type: L19R
	           Left Nuclease
	         TTTTCTGTCACCAATCCT
	5'-CTTTC TTTTCTGTAGCAAATTCT    AGATCTATTTTTTCTTATT     TCTTTGAGGTGGGGAGGA GTAGT-3'
	3'-GAAAG AAAAGACATCGTTTAAGA    TCTAGATAAAAAAGAATAA     AGAAACTCCACCCCTCCT CATCA-5'
	                                                       TGACACCCCACCTCCCCT
	                                                            Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 010-096170810
Forward Primer: 5'-GGCATGCCAGAGATAGGACTATTC-3' T_m: 59.038
Reverse Primer: 5'-GTGCTACAGTGGCAACCTTCATG-3' T_m: 59.148
Expected PCR Product Length: 330
Expected CelI Cleavage Product Sizes: 191, 139
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr10:96170972-96171026

Rank: 39	Homology Score: 2	RVD Match Score: 3.485	Left Half-Site RVD Score: 6.747	Right Half-Site RVD Score: 6.665
	Genomic Region: Intron	Closest Gene: KIRREL3
Match Type: R13L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-GTGTT TTCACTCACCACCACAGT       CAGGGCTGGCACA        GGGACTGGGGAATGAAAA ATAAA-3'
	3'-CACAA AAGTGAGTGGTGGTGTCA       GTCCCGACCGTGT        CCCTGACCCCTTACTTTT TATTT-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 011-126543310
Forward Primer: 5'-CCAGCAGCTCTGGAAATTGGATC-3' T_m: 59.148
Reverse Primer: 5'-GAGGAAGATCTTACAGACCCAACC-3' T_m: 59.038
Expected PCR Product Length: 345
Expected CelI Cleavage Product Sizes: 187, 158
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr11:126543471-126543519

Rank: 40	Homology Score: 2	RVD Match Score: 3.488	Left Half-Site RVD Score: 6.878	Right Half-Site RVD Score: 6.565
	Genomic Region: Intergenic	Closest Gene: UGT8
Match Type: L22R
	           Left Nuclease
	         TTTTCTGTCACCAATCCT
	5'-TATAG TTTTCTTTCAACATTCAC   TCAGGAAATGATTATGTGTGAG   TATGTGGATTTGAGGGGA AGTTA-3'
	3'-ATATC AAAAGAAAGTTGTAAGTG   AGTCCTTTACTAATACACACTC   ATACACCTAAACTCCCCT TCAAT-5'
	                                                       TGACACCCCACCTCCCCT
	                                                            Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 004-115491385
Forward Primer: 5'-CATGTTGCACAGGCTGAGTACTCT-3' T_m: 59.038
Reverse Primer: 5'-CCCATAGCTCTTTGGTCAGGTC-3' T_m: 59.258
Expected PCR Product Length: 399
Expected CelI Cleavage Product Sizes: 224, 175
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr4:115491578-115491635

Rank: 41	Homology Score: 2	RVD Match Score: 3.49	Left Half-Site RVD Score: 6.944	Right Half-Site RVD Score: 6.494
	Genomic Region: Intron	Closest Gene: GPC4
Match Type: R25L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-TGGAA TCCCCTGCACCCAGCATA CTGCTCTGGATACAGGAGCCATTCA  ATGATTGCTGATAATAAA TTTCT-3'
	3'-ACCTT AGGGGACGTGGGTCGTAT GACGAGACCTATGTCCTCGGTAAGT  TACTAACGACTATTATTT AAAGA-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 00X-132539743
Forward Primer: 5'-CTGCCATAGACTGGGAAACTGGA-3' T_m: 59.148
Reverse Primer: 5'-CCAGAACACAGCTGGGAATGTGGA-3' T_m: 60.908
Expected PCR Product Length: 283
Expected CelI Cleavage Product Sizes: 152, 131
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chrX:132539863-132539923

Rank: 42	Homology Score: 2	RVD Match Score: 3.49	Left Half-Site RVD Score: 6.57	Right Half-Site RVD Score: 6.871
	Genomic Region: Intergenic	Closest Gene: LMO1
Match Type: L22R
	           Left Nuclease
	         TTTTCTGTCACCAATCCT
	5'-ACCTT TATACTGTCCCCAAGCCC   CCACTTATCAAAACACATTCTG   ATTGAGGGGTAGAGAGAA GGGAG-3'
	3'-TGGAA ATATGACAGGGGTTCGGG   GGTGAATAGTTTTGTGTAAGAC   TAACTCCCCATCTCTCTT CCCTC-5'
	                                                       TGACACCCCACCTCCCCT
	                                                            Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 011-008291463
Forward Primer: 5'-GTTTCCTCGGGCAATGAATGAGC-3' T_m: 59.148
Reverse Primer: 5'-GCACAGTCCTCCTTTCTTCACC-3' T_m: 59.258
Expected PCR Product Length: 311
Expected CelI Cleavage Product Sizes: 155, 156
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr11:8291587-8291644

Rank: 43	Homology Score: 2	RVD Match Score: 3.492	Left Half-Site RVD Score: 7.689	Right Half-Site RVD Score: 5.787
	Genomic Region: Intron	Closest Gene: LYPD6B
Match Type: R23L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-CCTTA TCTCCTCCTTCCTAAAGT  AAGGTCTTTAGATATTCTTTTGA   AGTTTTGTTGATAGTAAA CTCCT-3'
	3'-GGAAT AGAGGAGGAAGGATTTCA  TTCCAGAAATCTATAAGAAAACT   TCAAAACAACTATCATTT GAGGA-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 002-149982161
Forward Primer: 5'-GGCATCTTCGAGTACCTTAACTCC-3' T_m: 59.038
Reverse Primer: 5'-CTCTTCTCAAGTTCAAGGGTCAGTTGTAC-3' T_m: 60.178
Expected PCR Product Length: 409
Expected CelI Cleavage Product Sizes: 219, 190
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr2:149982349-149982407

Rank: 44	Homology Score: 2	RVD Match Score: 3.492	Left Half-Site RVD Score: 6.687	Right Half-Site RVD Score: 6.779
	Genomic Region: Intergenic	Closest Gene: POM121L12
Match Type: L23R
	           Left Nuclease
	         TTTTCTGTCACCAATCCT
	5'-CTGAA TTTGCAGCCCCAAATCCT  GCCCACTGAGCAGACTTCTTTCT   ACCTTGGGGTGGGGTTGA AAGCG-3'
	3'-GACTT AAACGTCGGGGTTTAGGA  CGGGTGACTCGTCTGAAGAAAGA   TGGAACCCCACCCCAACT TTCGC-5'
	                                                       TGACACCCCACCTCCCCT
	                                                            Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 007-052309823
Forward Primer: 5'-CCTGAGTCCCTGCTGCATTAATTC-3' T_m: 59.038
Reverse Primer: 5'-ACTCTGTGTCCTCAACGGTGTTC-3' T_m: 59.148
Expected PCR Product Length: 341
Expected CelI Cleavage Product Sizes: 210, 131
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr7:52310002-52310060

Rank: 45	Homology Score: 5	RVD Match Score: 3.494	Left Half-Site RVD Score: 6.765	Right Half-Site RVD Score: 6.717
	Genomic Region: Intron	Closest Gene: LOC100188947
Match Type: R17L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-TCTTC TGCCTGCCACCCCTCAGT     GGAAGCCTGGCAGAGAG      TGGATGGCTGTCAGAAGA TGCTG-3'
	3'-AGAAG ACGGACGGTGGGGAGTCA     CCTTCGGACCGTCTCTC      ACCTACCGACAGTCTTCT ACGAC-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 010-093286041
Forward Primer: 5'-GATTCTGAGCAGGGAGCTTGG-3' T_m: 59.378
Reverse Primer: 5'-ACAGGATGAGCTAGGGCCTGA-3' T_m: 59.378
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 135, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr10:93286148-93286200

Rank: 46	Homology Score: 2	RVD Match Score: 3.497	Left Half-Site RVD Score: 6.825	Right Half-Site RVD Score: 6.682
	Genomic Region: Intron	Closest Gene: C16orf46
Match Type: L17R
	           Left Nuclease
	         TTTTCTGTCACCAATCCT
	5'-GTTTA TTTTCTTTTACCAATAAC     ATGTTTTGCTTCAAAAT      AATGTGAGGTTGAGGTTA GGGAG-3'
	3'-CAAAT AAAAGAAAATGGTTATTG     TACAAAACGAAGTTTTA      TTACACTCCAACTCCAAT CCCTC-5'
	                                                       TGACACCCCACCTCCCCT
	                                                            Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 016-081100645
Forward Primer: 5'-GCCCCCAAAGTGCATGTTTGGA-3' T_m: 59.258
Reverse Primer: 5'-GAGCTCTCCGATAACATCATGGC-3' T_m: 59.148
Expected PCR Product Length: 420
Expected CelI Cleavage Product Sizes: 244, 176
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr16:81100861-81100913

Rank: 47	Homology Score: 5	RVD Match Score: 3.498	Left Half-Site RVD Score: 6.409	Right Half-Site RVD Score: 7.134
	Genomic Region: Exon	Closest Gene: CDKAL1
Match Type: R16L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-TTCCC TCCCCTTCTCTCCACAGA      TTGGTGAAAGACAACA      AGTGTTAGTAACAGAAGA ATCTT-3'
	3'-AAGGG AGGGGAAGAGAGGTGTCT      AACCACTTTCTGTTGT      TCACAATCATTGTCTTCT TAGAA-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 006-021198081
Forward Primer: 5'-CCGTGATTTGAACAGGGTTCGC-3' T_m: 59.258
Reverse Primer: 5'-CTCTCCCCTTTAAATGCCCTTTGC-3' T_m: 59.038
Expected PCR Product Length: 341
Expected CelI Cleavage Product Sizes: 182, 159
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr6:21198235-21198286

Rank: 48	Homology Score: 2	RVD Match Score: 3.499	Left Half-Site RVD Score: 7.07	Right Half-Site RVD Score: 6.471
	Genomic Region: Intergenic	Closest Gene: ZNRF2
Match Type: L19R
	           Left Nuclease
	         TTTTCTGTCACCAATCCT
	5'-TCCCA TCCTCTGTCATCTATCCC    TACAATCTGCAGCCTGTGT     GGTGTGGGGGGCAGGAGA AGATT-3'
	3'-AGGGT AGGAGACAGTAGATAGGG    ATGTTAGACGTCGGACACA     CCACACCCCCCGTCCTCT TCTAA-5'
	                                                       TGACACCCCACCTCCCCT
	                                                            Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 007-030310150
Forward Primer: 5'-CTGCTGCCAAAAGCTACCCATG-3' T_m: 59.258
Reverse Primer: 5'-GAGCTCCAGTTTCCTGCACTGT-3' T_m: 59.258
Expected PCR Product Length: 386
Expected CelI Cleavage Product Sizes: 130, 256
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr7:30310251-30310305

Rank: 49	Homology Score: 2	RVD Match Score: 3.5	Left Half-Site RVD Score: 6.804	Right Half-Site RVD Score: 6.717
	Genomic Region: Intergenic	Closest Gene: NKX6-1
Match Type: R23L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-ACCAG TCGACACCACCCCACATG  CATTTTCTTTTTGATCCTGATCT   TGCATGGGTGACTGATAA TGAAA-3'
	3'-TGGTC AGCTGTGGTGGGGTGTAC  GTAAAAGAAAAACTAGGACTAGA   ACGTACCCACTGACTATT ACTTT-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 004-085429094
Forward Primer: 5'-GTCTCCCTTGCCAGACTATAACC-3' T_m: 59.148
Reverse Primer: 5'-CATGGCCAAGAGCTGTGCTTTC-3' T_m: 59.258
Expected PCR Product Length: 342
Expected CelI Cleavage Product Sizes: 146, 196
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr4:85429209-85429267

Rank: 50	Homology Score: 5	RVD Match Score: 3.501	Left Half-Site RVD Score: 6.21	Right Half-Site RVD Score: 7.316
	Genomic Region: Intergenic	Closest Gene: NEK7
Match Type: L25R
	           Left Nuclease
	         TTTTCTGTCACCAATCCT
	5'-GCTGA TTTCCTGACACCAAGGCT CCTCAGCTGAGGCTCCCACCTGCAA  GCTGTGAGCTGGAGATGA ATATC-3'
	3'-CGACT AAAGGACTGTGGTTCCGA GGAGTCGACTCCGAGGGTGGACGTT  CGACACTCGACCTCTACT TATAG-5'
	                                                       TGACACCCCACCTCCCCT
	                                                            Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 001-198360906
Forward Primer: 5'-GGTTTTATAGCCCTTCTCCTGCC-3' T_m: 59.148
Reverse Primer: 5'-GTTGTGGGATTTGAGCTGGTGG-3' T_m: 59.258
Expected PCR Product Length: 300
Expected CelI Cleavage Product Sizes: 130, 170
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr1:198361004-198361064

Rank: 51	Homology Score: 2	RVD Match Score: 3.501	Left Half-Site RVD Score: 6.551	Right Half-Site RVD Score: 7.004
	Genomic Region: Intergenic	Closest Gene: ADCK2
Match Type: R22L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-TCTAG TCCCCTGCATCCCGCAAC   ATTTCTTGGCGAGCCAGCCAGG   AGGAGTGGAGACGGCAGA TCTAC-3'
	3'-AGATC AGGGGACGTAGGGCGTTG   TAAAGAACCGCTCGGTCGGTCC   TCCTCACCTCTGCCGTCT AGATG-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 007-140355184
Forward Primer: 5'-CCCACAAGGGCCTTATTTTCAGTC-3' T_m: 59.038
Reverse Primer: 5'-GAGAGCTGATTTCACCCCTCTAG-3' T_m: 59.148
Expected PCR Product Length: 369
Expected CelI Cleavage Product Sizes: 236, 133
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr7:140355389-140355446

Rank: 52	Homology Score: 2	RVD Match Score: 3.502	Left Half-Site RVD Score: 7.159	Right Half-Site RVD Score: 6.371
	Genomic Region: Intergenic	Closest Gene: FAM9C
Match Type: R22L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-GAATT TCCCTTCTATCCCCCAGA   TCCACACTTCTCTATTCAAGTC   AGGGTTTGTGACAGGGTA GCCTA-3'
	3'-CTTAA AGGGAAGATAGGGGGTCT   AGGTGTGAAGAGATAAGTTCAG   TCCCAAACACTGTCCCAT CGGAT-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 00X-013108270
Forward Primer: 5'-GCCATTTTGGCAGAGCCACTGA-3' T_m: 59.258
Reverse Primer: 5'-GCATGTTGTGCACGAAATGACCTC-3' T_m: 59.038
Expected PCR Product Length: 271
Expected CelI Cleavage Product Sizes: 131, 140
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chrX:13108370-13108427

Rank: 53	Homology Score: 2	RVD Match Score: 3.502	Left Half-Site RVD Score: 7.134	Right Half-Site RVD Score: 6.437
	Genomic Region: Intron	Closest Gene: VIT
Match Type: L21R
	           Left Nuclease
	         TTTTCTGTCACCAATCCT
	5'-ACACG TTCTTTGTAATCAACCCT   AGGATATCAAAATTGGAAACA    GTTGTGGGATGAAGGGAA CTGTT-3'
	3'-TGTGC AAGAAACATTAGTTGGGA   TCCTATAGTTTTAACCTTTGT    CAACACCCTACTTCCCTT GACAA-5'
	                                                       TGACACCCCACCTCCCCT
	                                                            Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 002-037018108
Forward Primer: 5'-TTTCAGCTGCATCCCGGACC-3' T_m: 59.508
Reverse Primer: 5'-AGGAGATGCGGCCAAGTCAG-3' T_m: 59.508
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 135, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr2:37018213-37018269

Rank: 54	Homology Score: 2	RVD Match Score: 3.502	Left Half-Site RVD Score: 6.564	Right Half-Site RVD Score: 6.997
	Genomic Region: Intergenic	Closest Gene: HERC6
Match Type: R14L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-TGCCC TCCCATCCCCACCCCAGG       GGGCTTCCATGTCA       CAGATAGGTGAGAGATAA ATGGT-3'
	3'-ACGGG AGGGTAGGGGTGGGGTCC       CCCGAAGGTACAGT       GTCTATCCACTCTCTATT TACCA-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 004-089270564
Forward Primer: 5'-GTGGTTGGGGCACAGCTTAG-3' T_m: 59.508
Reverse Primer: 5'-CACACCTGCCTCCCATTCTATTC-3' T_m: 59.148
Expected PCR Product Length: 298
Expected CelI Cleavage Product Sizes: 165, 133
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr4:89270702-89270751

Rank: 55	Homology Score: 2	RVD Match Score: 3.502	Left Half-Site RVD Score: 6.926	Right Half-Site RVD Score: 6.612
	Genomic Region: Intron	Closest Gene: MAPT
Match Type: R24L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-TTCCT TACCTCCCACCCCACTGC  ATCTAAGTCACTGCCTGAGAAAAC  AGGATTGAGGAAAGGAGA AAGGA-3'
	3'-AAGGA ATGGAGGGTGGGGTGACG  TAGATTCAGTGACGGACTCTTTTG  TCCTAACTCCTTTCCTCT TTCCT-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 017-044029096
Forward Primer: 5'-GGACGGACTCAGGAGGCAAT-3' T_m: 59.508
Reverse Primer: 5'-CACACCAAGGCTGTTTTCCTCAGA-3' T_m: 59.038
Expected PCR Product Length: 266
Expected CelI Cleavage Product Sizes: 136, 130
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr17:44029200-44029259

Rank: 56	Homology Score: 2	RVD Match Score: 3.502	Left Half-Site RVD Score: 6.612	Right Half-Site RVD Score: 6.926
	Genomic Region: Intron	Closest Gene: MAPT
Match Type: L24R
	           Left Nuclease
	         TTTTCTGTCACCAATCCT
	5'-TCCTT TCTCCTTTCCTCAATCCT  GTTTTCTCAGGCAGTGACTTAGAT  GCAGTGGGGTGGGAGGTA AGGAA-3'
	3'-AGGAA AGAGGAAAGGAGTTAGGA  CAAAAGAGTCCGTCACTGAATCTA  CGTCACCCCACCCTCCAT TCCTT-5'
	                                                       TGACACCCCACCTCCCCT
	                                                            Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: ap1-000838311
Forward Primer: 5'-ACCAAGGCTGTTTTCCTCAGACC-3' T_m: 59.148
Reverse Primer: 5'-AACCACAGGGACGGACTCAG-3' T_m: 59.508
Expected PCR Product Length: 271
Expected CelI Cleavage Product Sizes: 131, 140
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr17_ctg5_hap1:838410-838469

Rank: 57	Homology Score: 5	RVD Match Score: 3.508	Left Half-Site RVD Score: 7.143	Right Half-Site RVD Score: 6.473
	Genomic Region: Intron	Closest Gene: PPP2R3A
Match Type: L21R
	           Left Nuclease
	         TTTTCTGTCACCAATCCT
	5'-CCTCG TTCTCTGTCTCCCTTCCC   TTTCAAACTCTGCTTCTCAGC    ACTTTGGGGTAAAAGGGA GGGGT-3'
	3'-GGAGC AAGAGACAGAGGGAAGGG   AAAGTTTGAGACGAAGAGTCG    TGAAACCCCATTTTCCCT CCCCA-5'
	                                                       TGACACCCCACCTCCCCT
	                                                            Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 003-135693383
Forward Primer: 5'-CTGTGCTTGCTTTGTCCCAGTC-3' T_m: 59.258
Reverse Primer: 5'-CCTTGCTAGGATTCCTAAGCCC-3' T_m: 59.258
Expected PCR Product Length: 377
Expected CelI Cleavage Product Sizes: 139, 238
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr3:135693492-135693548

Rank: 58	Homology Score: 2	RVD Match Score: 3.508	Left Half-Site RVD Score: 6.21	Right Half-Site RVD Score: 7.372
	Genomic Region: Intergenic	Closest Gene: TSPAN8
Match Type: L11R
	           Left Nuclease
	         TTTTCTGTCACCAATCCT
	5'-TGAAT TACTCTTTCACCAACCCA        CTTAGTGACCA         ACTATGGTTTGAAGGAGA CTGCA-3'
	3'-ACTTA ATGAGAAAGTGGTTGGGT        GAATCACTGGT         TGATACCAAACTTCCTCT GACGT-5'
	                                                       TGACACCCCACCTCCCCT
	                                                            Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 012-071464141
Forward Primer: 5'-CCACAAGTTTGGGGAGCAAGAC-3' T_m: 59.258
Reverse Primer: 5'-AAGCAAATGCCTAGTGTGGGAAGC-3' T_m: 59.038
Expected PCR Product Length: 290
Expected CelI Cleavage Product Sizes: 159, 131
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr12:71464275-71464321

Rank: 59	Homology Score: 2	RVD Match Score: 3.509	Left Half-Site RVD Score: 6.269	Right Half-Site RVD Score: 7.382
	Genomic Region: Intron	Closest Gene: NCMAP
Match Type: R17L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-CCTTG TCCCCTCCCCACCCAAGC     ATCTGGCGGAGGGGCCG      AGGCTCGCTGGAAGAAAA GGGAG-3'
	3'-GGAAC AGGGGAGGGGTGGGTTCG     TAGACCGCCTCCCCGGC      TCCGAGCGACCTTCTTTT CCCTC-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 001-024882812
Forward Primer: 5'-CATCTGTTGGAGGAAGTGCCTG-3' T_m: 59.258
Reverse Primer: 5'-CTCTGCATAGGGCAGACCCA-3' T_m: 59.508
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 137, 133
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr1:24882921-24882973

Rank: 60	Homology Score: 2	RVD Match Score: 3.513	Left Half-Site RVD Score: 6.924	Right Half-Site RVD Score: 6.697
	Genomic Region: Intergenic	Closest Gene: STIM2
Match Type: R22L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-GTCCC TCCTCTCCTCCCCATTAT   CTGTGGAACATTGACTTCATAT   TGCTTTGGTGACAGATTA TAGGG-3'
	3'-CAGGG AGGAGAGGAGGGGTAATA   GACACCTTGTAACTGAAGTATA   ACGAAACCACTGTCTAAT ATCCC-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 004-027261444
Forward Primer: 5'-GTGGGTTAAGAGAGGTATTTGGCC-3' T_m: 59.038
Reverse Primer: 5'-CCTATCCAAGCCACGATTAACTCC-3' T_m: 59.038
Expected PCR Product Length: 410
Expected CelI Cleavage Product Sizes: 210, 200
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr4:27261623-27261680

Rank: 61	Homology Score: 5	RVD Match Score: 3.513	Left Half-Site RVD Score: 6.717	Right Half-Site RVD Score: 6.905
	Genomic Region: Intron	Closest Gene: KCNT1
Match Type: L14R
	           Left Nuclease
	         TTTTCTGTCACCAATCCT
	5'-GTGGC TTCCCCGTCACCACTGCT       GTGGCCCACTGCCC       ACTGAGCAGAGGAGGGGA CGGGG-3'
	3'-CACCG AAGGGGCAGTGGTGACGA       CACCGGGTGACGGG       TGACTCGTCTCCTCCCCT GCCCC-5'
	                                                       TGACACCCCACCTCCCCT
	                                                            Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 009-138652006
Forward Primer: 5'-GGGAAGGGTCAAGGTGGACA-3' T_m: 59.508
Reverse Primer: 5'-AATAGCAGGGTCCCTCCAGGGT-3' T_m: 61.288
Expected PCR Product Length: 287
Expected CelI Cleavage Product Sizes: 131, 156
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr9:138652110-138652159

Rank: 62	Homology Score: 2	RVD Match Score: 3.515	Left Half-Site RVD Score: 7.138	Right Half-Site RVD Score: 6.494
	Genomic Region: Intron	Closest Gene: RYR3
Match Type: R24L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-CTCAA TCCCCATCATCCTACAGA  AATGACTTTAAAGTGAGAGCAAAC  AGCATTTGTGATGAAAAA AATGC-3'
	3'-GAGTT AGGGGTAGTAGGATGTCT  TTACTGAAATTTCACTCTCGTTTG  TCGTAAACACTACTTTTT TTACG-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 015-033799697
Forward Primer: 5'-GGCACTGTGGCCAATTTTATGGCT-3' T_m: 59.038
Reverse Primer: 5'-CCCAGTCCTACGAGATAGATTGC-3' T_m: 59.148
Expected PCR Product Length: 361
Expected CelI Cleavage Product Sizes: 193, 168
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr15:33799858-33799917

Rank: 63	Homology Score: 2	RVD Match Score: 3.517	Left Half-Site RVD Score: 7.273	Right Half-Site RVD Score: 6.371
	Genomic Region: Intergenic	Closest Gene: DIAPH2
Match Type: R11L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-CAAAC TCCCCTCTACCCCCGTGA        TGCTCAGACTA         GGTATTGGTGTCAGGGAA AAAGG-3'
	3'-GTTTG AGGGGAGATGGGGGCACT        ACGAGTCTGAT         CCATAACCACAGTCCCTT TTTCC-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 00X-096909115
Forward Primer: 5'-TAGAGAATGGTGTTTGGGGCTGC-3' T_m: 59.148
Reverse Primer: 5'-GGCTTGTATCTCAAAACTCCCACAGATAAC-3' T_m: 60.058
Expected PCR Product Length: 325
Expected CelI Cleavage Product Sizes: 130, 195
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chrX:96909220-96909266

Rank: 64	Homology Score: 2	RVD Match Score: 3.517	Left Half-Site RVD Score: 7.143	Right Half-Site RVD Score: 6.543
	Genomic Region: Intergenic	Closest Gene: CD83
Match Type: L16R
	           Left Nuclease
	         TTTTCTGTCACCAATCCT
	5'-GAAGG TTCTCTGTCCCCTTTCCA      GGGATTCTCAGTAATC      CCTGTGGTGGGGGTGGGA AGGCA-3'
	3'-CTTCC AAGAGACAGGGGAAAGGT      CCCTAAGAGTCATTAG      GGACACCACCCCCACCCT TCCGT-5'
	                                                       TGACACCCCACCTCCCCT
	                                                            Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 006-014315745
Forward Primer: 5'-GGAAGGAAGGAAGGAAGGAGAAAG-3' T_m: 59.038
Reverse Primer: 5'-CCCTGCTTGTTTTTAAGGGGCC-3' T_m: 59.258
Expected PCR Product Length: 379
Expected CelI Cleavage Product Sizes: 215, 164
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr6:14315932-14315983

Rank: 65	Homology Score: 2	RVD Match Score: 3.519	Left Half-Site RVD Score: 7.052	Right Half-Site RVD Score: 6.611
	Genomic Region: Intron	Closest Gene: MYO1D
Match Type: R24L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-TATTG TCCTCAGCACACAACAGT  ACCTATACAGTAGAATAAGAACCA  AAGATTGTTTATACAAAA ATTAA-3'
	3'-ATAAC AGGAGTCGTGTGTTGTCA  TGGATATGTCATCTTATTCTTGGT  TTCTAACAAATATGTTTT TAATT-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 017-031174782
Forward Primer: 5'-CTCAGCCTCCCAAAATGCTGAGA-3' T_m: 59.148
Reverse Primer: 5'-CGGAGAGAGGCCCATTTTTCATAG-3' T_m: 59.038
Expected PCR Product Length: 344
Expected CelI Cleavage Product Sizes: 144, 200
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr17:31174894-31174953

Rank: 66	Homology Score: 5	RVD Match Score: 3.52	Left Half-Site RVD Score: 5.589	Right Half-Site RVD Score: 8.138
	Genomic Region: Intergenic	Closest Gene: TSPAN9
Match Type: L12R
	           Left Nuclease
	         TTTTCTGTCACCAATCCT
	5'-TTGGA TTTTCTGACACAAACCCA        TCAGGATCTGGA        ACAGTGGCGTCCAGGAGA ACTTT-3'
	3'-AACCT AAAAGACTGTGTTTGGGT        AGTCCTAGACCT        TGTCACCGCAGGTCCTCT TGAAA-5'
	                                                       TGACACCCCACCTCCCCT
	                                                            Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 012-003414995
Forward Primer: 5'-TGACGTTCCCTGGGCCAAGA-3' T_m: 59.508
Reverse Primer: 5'-AAAATCCAGTTCCTTGCTCGCACC-3' T_m: 59.038
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 132, 138
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr12:3415101-3415148

Rank: 67	Homology Score: 2	RVD Match Score: 3.522	Left Half-Site RVD Score: 6.513	Right Half-Site RVD Score: 7.216
	Genomic Region: Intergenic	Closest Gene: NOVA1
Match Type: R18L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-AATTA TATCCACCACCCCCCAGA     AAATAAGAGGCCAGAACA     AGCATGGTTGACAAGAAA AGTTT-3'
	3'-TTAAT ATAGGTGGTGGGGGGTCT     TTTATTCTCCGGTCTTGT     TCGTACCAACTGTTCTTT TCAAA-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Warning! Primer pair was generated using suboptimal parameters. 
Primer Index: 014-026411302
Forward Primer: 5'-CCAGTCCCAAGGATTCCAGTTTAG-3' T_m: 59.038
Reverse Primer: 5'-TTCCAAATAAAATGATCTCTCCTTCCTTTGAATCTGGC-3' T_m: 60.528
Expected PCR Product Length: 400
Expected CelI Cleavage Product Sizes: 270, 130
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr14:26411543-26411596

Rank: 68	Homology Score: 2	RVD Match Score: 3.523	Left Half-Site RVD Score: 6.808	Right Half-Site RVD Score: 6.9
	Genomic Region: Intron	Closest Gene: ANKRD36
Match Type: R26L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-AACAT TCCCTCCCAACCCCCAGG AAAAAAAGAGTTGGTAAAGGAGAATG AGCAGTGGCTAGAGAAAA CTAGG-3'
	3'-TTGTA AGGGAGGGTTGGGGGTCC TTTTTTTCTCAACCATTTCCTCTTAC TCGTCACCGATCTCTTTT GATCC-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 002-097793909
Forward Primer: 5'-GGTTGGAGATGCAGACGTAGAATG-3' T_m: 59.038
Reverse Primer: 5'-GAAAAGGGTACCGCCAAAGAGC-3' T_m: 59.258
Expected PCR Product Length: 334
Expected CelI Cleavage Product Sizes: 163, 171
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr2:97794039-97794100

Rank: 69	Homology Score: 2	RVD Match Score: 3.524	Left Half-Site RVD Score: 6.505	Right Half-Site RVD Score: 7.239
	Genomic Region: Intron	Closest Gene: PARP1
Match Type: R17L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-ACATC TCCTCTCCATCACACACA     GCATACACTGGGACACC      AGGAATGGAGACATGAGA GGATG-3'
	3'-TGTAG AGGAGAGGTAGTGTGTGT     CGTATGTGACCCTGTGG      TCCTTACCTCTGTACTCT CCTAC-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 001-226575130
Forward Primer: 5'-GGAGACAAAAATGCCAGCCCTATC-3' T_m: 59.038
Reverse Primer: 5'-CCACTGTGGCACATCTGTGTC-3' T_m: 59.378
Expected PCR Product Length: 300
Expected CelI Cleavage Product Sizes: 169, 131
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr1:226575271-226575323

Rank: 70	Homology Score: 2	RVD Match Score: 3.525	Left Half-Site RVD Score: 6.956	Right Half-Site RVD Score: 6.761
	Genomic Region: Intergenic	Closest Gene: TBRG4
Match Type: R25L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-TGGAT TCCTCTCGACCCCCAAGC CAGGAGCACAGCCAGGGTGTAGAAA  GTGTTTGGAGAAAGAAAA TCCCA-3'
	3'-ACCTA AGGAGAGCTGGGGGTTCG GTCCTCGTGTCGGTCCCACATCTTT  CACAAACCTCTTTCTTTT AGGGT-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 007-045162226
Forward Primer: 5'-GGGGAGGCTGGTGATTTTTTTGAG-3' T_m: 59.038
Reverse Primer: 5'-GCTGAACACGGTATACAAGGCAAC-3' T_m: 59.038
Expected PCR Product Length: 284
Expected CelI Cleavage Product Sizes: 145, 139
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr7:45162339-45162399

Rank: 71	Homology Score: 5	RVD Match Score: 3.526	Left Half-Site RVD Score: 7.687	Right Half-Site RVD Score: 6.044
	Genomic Region: Intergenic	Closest Gene: CD6
Match Type: R22L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-TGGCT TCCCCTCCACTCCTGGGA   GACCTCATTATAATACGCCTCA   AGGATCTGGGACAGGAAA CACAA-3'
	3'-ACCGA AGGGGAGGTGAGGACCCT   CTGGAGTAATATTATGCGGAGT   TCCTAGACCCTGTCCTTT GTGTT-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 011-060790178
Forward Primer: 5'-GCCTGTGCTGAGACTGGGAA-3' T_m: 59.508
Reverse Primer: 5'-AGCTGTCAGTCAAATTGCTGGGTG-3' T_m: 59.038
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 131, 139
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr11:60790278-60790335

Rank: 72	Homology Score: 2	RVD Match Score: 3.526	Left Half-Site RVD Score: 6.409	Right Half-Site RVD Score: 7.365
	Genomic Region: Intergenic	Closest Gene: TNRC18
Match Type: R14L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-CCCCC TCCCCACCCCCTCATACT       TTTGTTTACCCAGC       GGGATAGGTGGCCAAAAA CTGTG-3'
	3'-GGGGG AGGGGTGGGGGAGTATGA       AAACAAATGGGTCG       CCCTATCCACCGGTTTTT GACAC-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 007-005466629
Forward Primer: 5'-AAATGTAAAAACCAGGCCTGGGCG-3' T_m: 59.038
Reverse Primer: 5'-CCCCAGCCGATTTTCTACCC-3' T_m: 59.508
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 133, 137
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr7:5466735-5466784

Rank: 73	Homology Score: 2	RVD Match Score: 3.529	Left Half-Site RVD Score: 6.482	Right Half-Site RVD Score: 7.308
	Genomic Region: Intron	Closest Gene: LRRC49
Match Type: R18L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-TAATT TACACTCCCACCAACAGT     GTAAAAGAACTTCACCCC     AGCCTGGGTGACAGAGCA AGACC-3'
	3'-ATTAA ATGTGAGGGTGGTTGTCA     CATTTTCTTGAAGTGGGG     TCGGACCCACTGTCTCGT TCTGG-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Warning! Primer pair was generated using suboptimal parameters. 
Primer Index: 015-071318700
Forward Primer: 5'-GTGCTGCAATAAACATACATGTGCATGTGTC-3' T_m: 59.958
Reverse Primer: 5'-CCCCATCAAAAAGTGGGTGAAGG-3' T_m: 59.148
Expected PCR Product Length: 425
Expected CelI Cleavage Product Sizes: 188, 237
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr15:71318859-71318912

Rank: 74	Homology Score: 2	RVD Match Score: 3.529	Left Half-Site RVD Score: 6.813	Right Half-Site RVD Score: 6.937
	Genomic Region: Intron	Closest Gene: PVT1
Match Type: L24R
	           Left Nuclease
	         TTTTCTGTCACCAATCCT
	5'-ATAAA TTTCCTGACACCAAAAGT  CAGTTCAAGTGAACTAGAATGGGC  AGAGAGGGGTCGTGGGGA TGGTT-3'
	3'-TATTT AAAGGACTGTGGTTTTCA  GTCAAGTTCACTTGATCTTACCCG  TCTCTCCCCAGCACCCCT ACCAA-5'
	                                                       TGACACCCCACCTCCCCT
	                                                            Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 008-129090046
Forward Primer: 5'-CATCCTCCCAGCCATCAATACC-3' T_m: 59.258
Reverse Primer: 5'-AGGCTGGGACCACAGGCATA-3' T_m: 59.508
Expected PCR Product Length: 304
Expected CelI Cleavage Product Sizes: 174, 130
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr8:129090188-129090247

Rank: 75	Homology Score: 2	RVD Match Score: 3.53	Left Half-Site RVD Score: 6.86	Right Half-Site RVD Score: 6.9
	Genomic Region: Intergenic	Closest Gene: TTTY3B
Match Type: R26L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-CAACA TTCCCTCCCGCCCCCAGA AAAAAAAGAGTTGGTAAAGGAGAATG AGCAGTGGCTAGAGAAAA GTAGG-3'
	3'-GTTGT AAGGGAGGGCGGGGGTCT TTTTTTTCTCAACCATTTCCTCTTAC TCGTCACCGATCTCTTTT CATCC-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 00Y-028570220
Forward Primer: 5'-CTGAGGTTGGAGATGCAGAAGTAG-3' T_m: 59.038
Reverse Primer: 5'-GAAAACGGTACCGCCAAAGAGC-3' T_m: 59.258
Expected PCR Product Length: 333
Expected CelI Cleavage Product Sizes: 162, 171
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chrY:28570349-28570410

Rank: 76	Homology Score: 2	RVD Match Score: 3.531	Left Half-Site RVD Score: 6.275	Right Half-Site RVD Score: 7.486
	Genomic Region: Intron	Closest Gene: SLC9A2
Match Type: L25R
	           Left Nuclease
	         TTTTCTGTCACCAATCCT
	5'-TTTCC TTCTCTGACACCACCCCA ATAGAAAAGCCAGGGTGTTCCTTAC  ACTCTGGTGAGGATGGAA TCTAA-3'
	3'-AAAGG AAGAGACTGTGGTGGGGT TATCTTTTCGGTCCCACAAGGAATG  TGAGACCACTCCTACCTT AGATT-5'
	                                                       TGACACCCCACCTCCCCT
	                                                            Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 002-103259635
Forward Primer: 5'-GTTCTCTGCGTTCTTTACGTCGTC-3' T_m: 59.038
Reverse Primer: 5'-GTCAACCTCACAAGGCACAGAAG-3' T_m: 59.148
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 130, 140
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr2:103259733-103259793

Rank: 77	Homology Score: 2	RVD Match Score: 3.533	Left Half-Site RVD Score: 6.769	Right Half-Site RVD Score: 7.011
	Genomic Region: Intergenic	Closest Gene: FLJ25363
Match Type: L21R
	           Left Nuclease
	         TTTTCTGTCACCAATCCT
	5'-TTTAA TTTCCTTTCAGCAAACAT   TTATTGAGTGCCAAGCATTGA    ACTAGGTGTTGGAAGGGA ATACA-3'
	3'-AAATT AAAGGAAAGTCGTTTGTA   AATAACTCACGGTTCGTAACT    TGATCCACAACCTTCCCT TATGT-5'
	                                                       TGACACCCCACCTCCCCT
	                                                            Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 003-109619335
Forward Primer: 5'-CAGGGAAGTCTCAGTGGTTTTTGG-3' T_m: 59.038
Reverse Primer: 5'-CCAGGTGTATTCTGTGTGGCTAG-3' T_m: 59.148
Expected PCR Product Length: 421
Expected CelI Cleavage Product Sizes: 266, 155
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr3:109619571-109619627

Rank: 78	Homology Score: 2	RVD Match Score: 3.537	Left Half-Site RVD Score: 7.094	Right Half-Site RVD Score: 6.71
	Genomic Region: Intron	Closest Gene: TMEM132D
Match Type: R12L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-GGGGT TTCTCTCCACCCCATATC        CCAAACCCTCTT        AGATTTGCTGAGAGAGAA TTTTG-3'
	3'-CCCCA AAGAGAGGTGGGGTATAG        GGTTTGGGAGAA        TCTAAACGACTCTCTCTT AAAAC-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 012-129962958
Forward Primer: 5'-CCACCATCTCATGTATGGTTGAGC-3' T_m: 59.038
Reverse Primer: 5'-GGGGATCAGAGCACATTCCAG-3' T_m: 59.378
Expected PCR Product Length: 337
Expected CelI Cleavage Product Sizes: 207, 130
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr12:129963139-129963186

Rank: 79	Homology Score: 2	RVD Match Score: 3.538	Left Half-Site RVD Score: 6.997	Right Half-Site RVD Score: 6.829
	Genomic Region: Intergenic	Closest Gene: KLF6
Match Type: L23R
	           Left Nuclease
	         TTTTCTGTCACCAATCCT
	5'-CTGCC TTTTCTCTCACTTAGCCC  TTCTCCTCAAAGCCCTCAGTATT   GATGTGGGATTGAGGAGA CGGGA-3'
	3'-GACGG AAAAGAGAGTGAATCGGG  AAGAGGAGTTTCGGGAGTCATAA   CTACACCCTAACTCCTCT GCCCT-5'
	                                                       TGACACCCCACCTCCCCT
	                                                            Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 010-003936310
Forward Primer: 5'-GGGTGATGTGGTTTTCAGCTGTTG-3' T_m: 59.038
Reverse Primer: 5'-TTCGCTAGGAATGGCCTCTACG-3' T_m: 59.258
Expected PCR Product Length: 301
Expected CelI Cleavage Product Sizes: 170, 131
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr10:3936449-3936507

Rank: 80	Homology Score: 2	RVD Match Score: 3.539	Left Half-Site RVD Score: 6.391	Right Half-Site RVD Score: 7.425
	Genomic Region: Intron	Closest Gene: TYW1
Match Type: L25R
	           Left Nuclease
	         TTTTCTGTCACCAATCCT
	5'-AAGCT TCTTCTGACACCACTCAA GTGTATGGGGGAGAGGTGCCTTGTT  ACTGCTAGGTGGAGTGAA AGTCC-3'
	3'-TTCGA AGAAGACTGTGGTGAGTT CACATACCCCCTCTCCACGGAACAA  TGACGATCCACCTCACTT TCAGG-5'
	                                                       TGACACCCCACCTCCCCT
	                                                            Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 007-066670329
Forward Primer: 5'-TGACTGTTCCCCCCTTAGACTC-3' T_m: 59.258
Reverse Primer: 5'-TTGACTCCTACTACCAGGGCG-3' T_m: 59.378
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 135, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr7:66670432-66670492

Rank: 81	Homology Score: 2	RVD Match Score: 3.542	Left Half-Site RVD Score: 6.133	Right Half-Site RVD Score: 7.716
	Genomic Region: Intergenic	Closest Gene: SLAIN2
Match Type: L21R
	           Left Nuclease
	         TTTTCTGTCACCAATCCT
	5'-CTACT TTCTCTATCACCACTGAT   ATGGACAGGAGACAGGGAAAT    ACTGGGCAGAGGAGGGCA TTCAT-3'
	3'-GATGA AAGAGATAGTGGTGACTA   TACCTGTCCTCTGTCCCTTTA    TGACCCGTCTCCTCCCGT AAGTA-5'
	                                                       TGACACCCCACCTCCCCT
	                                                            Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 004-048328999
Forward Primer: 5'-AAATCTCTTCCACCCCACCACTC-3' T_m: 59.148
Reverse Primer: 5'-TACAGGATAGGGGGGTGTGG-3' T_m: 59.508
Expected PCR Product Length: 281
Expected CelI Cleavage Product Sizes: 130, 151
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr4:48329099-48329155

Rank: 82	Homology Score: 2	RVD Match Score: 3.543	Left Half-Site RVD Score: 7.078	Right Half-Site RVD Score: 6.794
	Genomic Region: Intergenic	Closest Gene: C14orf132
Match Type: L21R
	           Left Nuclease
	         TTTTCTGTCACCAATCCT
	5'-AGCTC TGTTATGGCAACAAACCT   AAAATACGTGATACTTGCTTC    ATTGTGGGGTGACAGGCA TTGAG-3'
	3'-TCGAG ACAATACCGTTGTTTGGA   TTTTATGCACTATGAACGAAG    TAACACCCCACTGTCCGT AACTC-5'
	                                                       TGACACCCCACCTCCCCT
	                                                            Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 014-096450488
Forward Primer: 5'-GCTGCCCAAGCTCTTACAATGATC-3' T_m: 59.038
Reverse Primer: 5'-GTAAGCACATGGTCTGCTTCCG-3' T_m: 59.258
Expected PCR Product Length: 287
Expected CelI Cleavage Product Sizes: 147, 140
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr14:96450605-96450661

Rank: 83	Homology Score: 2	RVD Match Score: 3.544	Left Half-Site RVD Score: 7.142	Right Half-Site RVD Score: 6.743
	Genomic Region: Intergenic	Closest Gene: TAS2R42
Match Type: L16R
	           Left Nuclease
	         TTTTCTGTCACCAATCCT
	5'-GTATT TTTTCTTTTTCAAAACCT      ATGGGCTGTACTTTTC      CATGTGGGGTGATGGGTA ACAAG-3'
	3'-CATAA AAAAGAAAAAGTTTTGGA      TACCCGACATGAAAAG      GTACACCCCACTACCCAT TGTTC-5'
	                                                       TGACACCCCACCTCCCCT
	                                                            Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 012-011342495
Forward Primer: 5'-CTGTCTTCCAGTCTAAAGCAACTACCTC-3' T_m: 60.298
Reverse Primer: 5'-CCACACATCCTCTTCCTCCTATTC-3' T_m: 59.038
Expected PCR Product Length: 385
Expected CelI Cleavage Product Sizes: 132, 253
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr12:11342599-11342650

Rank: 84	Homology Score: 2	RVD Match Score: 3.547	Left Half-Site RVD Score: 7.021	Right Half-Site RVD Score: 6.869
	Genomic Region: Intron	Closest Gene: CHD2
Match Type: R19L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-AATAA TACCATCTACCCAACAGG    ATTATTAGAAACTATGCTA     AGATTTTGTGACATTAAA TAAAT-3'
	3'-TTATT ATGGTAGATGGGTTGTCC    TAATAATCTTTGATACGAT     TCTAAAACACTGTAATTT ATTTA-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 015-093472933
Forward Primer: 5'-GGTGTGGATCCCGTTGACAATCT-3' T_m: 59.148
Reverse Primer: 5'-GCCAAGTGCAGGTTTGCTTACAG-3' T_m: 59.148
Expected PCR Product Length: 406
Expected CelI Cleavage Product Sizes: 262, 144
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr15:93473166-93473220

Rank: 85	Homology Score: 2	RVD Match Score: 3.548	Left Half-Site RVD Score: 7.378	Right Half-Site RVD Score: 6.508
	Genomic Region: Intergenic	Closest Gene: HELQ
Match Type: R15L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-TTGTA TCCTCTGCAGCACACAGC      AAACCCTCAATAGAA       AGTATGGGTGTCATGAAA CAACT-3'
	3'-AACAT AGGAGACGTCGTGTGTCG      TTTGGGAGTTATCTT       TCATACCCACAGTACTTT GTTGA-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 004-084304603
Forward Primer: 5'-CCTACCTCTCCTCTTCAATTCCTC-3' T_m: 59.038
Reverse Primer: 5'-GCCTAGCTTCCAGAATCTGAACAC-3' T_m: 59.038
Expected PCR Product Length: 291
Expected CelI Cleavage Product Sizes: 151, 140
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr4:84304727-84304777

Rank: 86	Homology Score: 5	RVD Match Score: 3.55	Left Half-Site RVD Score: 6.479	Right Half-Site RVD Score: 7.489
	Genomic Region: Intergenic	Closest Gene: PJA1
Match Type: R21L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-GAGGA TCTCCTCCAGGCCACAGC   CCTACCTCACTCTGCAGTAGC    AGCAATGAGGAAAGAAAA ATGGT-3'
	3'-CTCCT AGAGGAGGTCCGGTGTCG   GGATGGAGTGAGACGTCATCG    TCGTTACTCCTTTCTTTT TACCA-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 00X-068408586
Forward Primer: 5'-GCACTCCTTTACCCTCCGATATC-3' T_m: 59.148
Reverse Primer: 5'-AGGTTTGAGGTGCACTACTTCAGG-3' T_m: 59.038
Expected PCR Product Length: 273
Expected CelI Cleavage Product Sizes: 142, 131
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chrX:68408698-68408754

Rank: 87	Homology Score: 2	RVD Match Score: 3.552	Left Half-Site RVD Score: 7.001	Right Half-Site RVD Score: 6.93
	Genomic Region: Intron	Closest Gene: DUSP22
Match Type: R19L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-AGAAC TTCCCTCAACCCCAGTCT    GTGGTAACTATGTGTTTCA     GGGATTTCTTACAGAGAA ATTCC-3'
	3'-TCTTG AAGGGAGTTGGGGTCAGA    CACCATTGATACACAAAGT     CCCTAAAGAATGTCTCTT TAAGG-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 006-000344885
Forward Primer: 5'-ACCTTCCTCACCTGCGTCAC-3' T_m: 59.508
Reverse Primer: 5'-AACCTGGTCATACTGTCTGTGGC-3' T_m: 59.148
Expected PCR Product Length: 276
Expected CelI Cleavage Product Sizes: 145, 131
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr6:345001-345055

Rank: 88	Homology Score: 2	RVD Match Score: 3.552	Left Half-Site RVD Score: 7.524	Right Half-Site RVD Score: 6.461
	Genomic Region: Intron	Closest Gene: PDE7B
Match Type: L21R
	           Left Nuclease
	         TTTTCTGTCACCAATCCT
	5'-ACTCT TTTTCTTTTAACAGTTCT   TTTAACTTTTGTAGCTACTGA    ACTTTGGGGTTTGGGGTA TCAAT-3'
	3'-TGAGA AAAAGAAAATTGTCAAGA   AAATTGAAAACATCGATGACT    TGAAACCCCAAACCCCAT AGTTA-5'
	                                                       TGACACCCCACCTCCCCT
	                                                            Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 006-136482738
Forward Primer: 5'-GGGAGGGAACCCATTGCAGT-3' T_m: 59.508
Reverse Primer: 5'-CTATTGAGGGCAGGGAGCGT-3' T_m: 59.508
Expected PCR Product Length: 289
Expected CelI Cleavage Product Sizes: 158, 131
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr6:136482866-136482922

Rank: 89	Homology Score: 5	RVD Match Score: 3.553	Left Half-Site RVD Score: 6.769	Right Half-Site RVD Score: 7.16
	Genomic Region: Intron	Closest Gene: ERC2
Match Type: L11R
	           Left Nuclease
	         TTTTCTGTCACCAATCCT
	5'-TTCAC TTTTCTGGGACAAATGCT        ACCAGAGTGCA         ACTGCTGGGTTGAATGGA AGTTG-3'
	3'-AAGTG AAAAGACCCTGTTTACGA        TGGTCTCACGT         TGACGACCCAACTTACCT TCAAC-5'
	                                                       TGACACCCCACCTCCCCT
	                                                            Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 003-056061208
Forward Primer: 5'-GGCATGGATATCCAGGCTTGCT-3' T_m: 59.258
Reverse Primer: 5'-TGGGGATGTACAGAGGCCAG-3' T_m: 59.508
Expected PCR Product Length: 292
Expected CelI Cleavage Product Sizes: 161, 131
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr3:56061344-56061390

Rank: 90	Homology Score: 2	RVD Match Score: 3.556	Left Half-Site RVD Score: 6.554	Right Half-Site RVD Score: 7.446
	Genomic Region: Exon	Closest Gene: SKIV2L2
Match Type: R24L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-TGTAT TCTCATCCACTTCACAAT  GATCCAAATTTGGAAACTGTGTAT  ACGCTTTGTGAAAAAAAA GCACA-3'
	3'-ACATA AGAGTAGGTGAAGTGTTA  CTAGGTTTAAACCTTTGACACATA  TGCGAAACACTTTTTTTT CGTGT-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 005-054696084
Forward Primer: 5'-CGGCATCCCCTTATTAGACCC-3' T_m: 59.378
Reverse Primer: 5'-CTGACAGGTTCAGAGCTGACAGT-3' T_m: 59.148
Expected PCR Product Length: 317
Expected CelI Cleavage Product Sizes: 132, 185
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr5:54696184-54696243

Rank: 91	Homology Score: 2	RVD Match Score: 3.556	Left Half-Site RVD Score: 6.873	Right Half-Site RVD Score: 7.099
	Genomic Region: Intron	Closest Gene: ITGB2
Match Type: R23L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-GGGAA TTCCTACCACCACACAGA  TGGTAGGCGGAAGTAGCCCCACG   AGGGTGGATCACAGTAAA ATCAC-3'
	3'-CCCTT AAGGATGGTGGTGTGTCT  ACCATCCGCCTTCATCGGGGTGC   TCCCACCTAGTGTCATTT TAGTG-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 021-046316063
Forward Primer: 5'-GATCCTGCCTGGTGGATTTCAAG-3' T_m: 59.148
Reverse Primer: 5'-ATGTGTGAGCATGGGGGAGG-3' T_m: 59.508
Expected PCR Product Length: 284
Expected CelI Cleavage Product Sizes: 130, 154
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr21:46316162-46316220

Rank: 92	Homology Score: 2	RVD Match Score: 3.568	Left Half-Site RVD Score: 6.9	Right Half-Site RVD Score: 7.155
	Genomic Region: Intron	Closest Gene: RNU6-76
Match Type: L26R
	           Left Nuclease
	         TTTTCTGTCACCAATCCT
	5'-CCTAC TTTTCTCTAGCCACTGCT CATTCTCCTTTACCAATTCTTTTTCT CCTGGGGGCGGGAGGGAA TGTTG-3'
	3'-GGATG AAAAGAGATCGGTGACGA GTAAGAGGAAATGGTTAAGAAAAAGA GGACCCCCGCCCTCCCTT ACAAC-5'
	                                                       TGACACCCCACCTCCCCT
	                                                            Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 016-033560648
Forward Primer: 5'-GTTCTTTTGAAAAGGGTACTGCCAAAGAGC-3' T_m: 60.058
Reverse Primer: 5'-GGTTGGAGATGCAGACGTAGAATG-3' T_m: 59.038
Expected PCR Product Length: 336
Expected CelI Cleavage Product Sizes: 182, 154
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr16:33560797-33560858

Rank: 93	Homology Score: 2	RVD Match Score: 3.569	Left Half-Site RVD Score: 6.782	Right Half-Site RVD Score: 7.272
	Genomic Region: Intergenic	Closest Gene: AFF2
Match Type: L11R
	           Left Nuclease
	         TTTTCTGTCACCAATCCT
	5'-TGGTT TATTCTTTCAACCTTCCT        GGAATACTGAG         AATGTCAGGTGGAGTGAA GTTTG-3'
	3'-ACCAA ATAAGAAAGTTGGAAGGA        CCTTATGACTC         TTACAGTCCACCTCACTT CAAAC-5'
	                                                       TGACACCCCACCTCCCCT
	                                                            Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 00X-147453463
Forward Primer: 5'-GCCACCCACAGATCATTTCAAAGG-3' T_m: 59.038
Reverse Primer: 5'-GGAAAGCTTTTCCCATGTGGACAG-3' T_m: 59.038
Expected PCR Product Length: 388
Expected CelI Cleavage Product Sizes: 194, 194
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chrX:147453632-147453678

Rank: 94	Homology Score: 2	RVD Match Score: 3.571	Left Half-Site RVD Score: 7.55	Right Half-Site RVD Score: 6.516
	Genomic Region: Exon	Closest Gene: HMCN1
Match Type: R23L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-AATTT TTCTCTCCACTCAACAGG  GTGGTGGTGATTGCCCAGAAATG   AGTATTGGAGCTATAAAA ATTGC-3'
	3'-TTAAA AAGAGAGGTGAGTTGTCC  CACCACCACTAACGGGTCTTTAC   TCATAACCTCGATATTTT TAACG-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 001-185833320
Forward Primer: 5'-CATCGTGTAAGAGAAGGCAGAGTG-3' T_m: 59.038
Reverse Primer: 5'-CTGTTGGATAAGTTGCAGCACCTC-3' T_m: 59.038
Expected PCR Product Length: 429
Expected CelI Cleavage Product Sizes: 296, 133
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr1:185833585-185833643

Rank: 95	Homology Score: 5	RVD Match Score: 3.573	Left Half-Site RVD Score: 7.642	Right Half-Site RVD Score: 6.509
	Genomic Region: Intron	Closest Gene: ZNF25
Match Type: L15R
	           Left Nuclease
	         TTTTCTGTCACCAATCCT
	5'-AAAAA TTTTTTGTCCCATTTCCT      GTCTTTAGAAACTAG       CCTCTGGGCTGGATGGGA TTCCC-3'
	3'-TTTTT AAAAAACAGGGTAAAGGA      CAGAAATCTTTGATC       GGAGACCCGACCTACCCT AAGGG-5'
	                                                       TGACACCCCACCTCCCCT
	                                                            Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 010-038264363
Forward Primer: 5'-GGTGCTTACGAAATGCCTAAGCTC-3' T_m: 59.038
Reverse Primer: 5'-AGGCCTTCCTGCATCCTCCA-3' T_m: 59.508
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 135, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr10:38264471-38264521

Rank: 96	Homology Score: 2	RVD Match Score: 3.573	Left Half-Site RVD Score: 6.871	Right Half-Site RVD Score: 7.238
	Genomic Region: Intergenic	Closest Gene: POU6F2
Match Type: R14L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-GAGTG TTTCTTCCTCCCCACAAT       GATGAAAAGGCCCC       AGGAGCTTTGACAGAACA AATGG-3'
	3'-CTCAC AAAGAAGGAGGGGTGTTA       CTACTTTTCCGGGG       TCCTCGAAACTGTCTTGT TTACC-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 007-039538608
Forward Primer: 5'-GCAGGGATGAATGTGATTCCACAG-3' T_m: 59.038
Reverse Primer: 5'-GAGATTGTGCTACTGCCCTCC-3' T_m: 59.378
Expected PCR Product Length: 281
Expected CelI Cleavage Product Sizes: 138, 143
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr7:39538719-39538768

Rank: 97	Homology Score: 2	RVD Match Score: 3.573	Left Half-Site RVD Score: 7.195	Right Half-Site RVD Score: 6.913
	Genomic Region: Intron	Closest Gene: FAM107B
Match Type: L13R
	           Left Nuclease
	         TTTTCTGTCACCAATCCT
	5'-TTTTT TTTTTTGAGACAAAACCT       CACTCTGTCACCC        AGGCTGGAGTGCAGGGGA GCAAT-3'
	3'-AAAAA AAAAAACTCTGTTTTGGA       GTGAGACAGTGGG        TCCGACCTCACGTCCCCT CGTTA-5'
	                                                       TGACACCCCACCTCCCCT
	                                                            Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 010-014726614
Forward Primer: 5'-GCATTGTGCCAAAAGGATCCCG-3' T_m: 59.258
Reverse Primer: 5'-GATAGGGAGACCCCATGTCTAC-3' T_m: 59.258
Expected PCR Product Length: 419
Expected CelI Cleavage Product Sizes: 244, 175
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr10:14726832-14726880

Rank: 98	Homology Score: 2	RVD Match Score: 3.576	Left Half-Site RVD Score: 7.096	Right Half-Site RVD Score: 7.026
	Genomic Region: Intergenic	Closest Gene: SOX4
Match Type: R21L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-AGTAT TCCCCTCCTCAACACGGC   TCCTCCAGTCCAAATAAGCAC    AGGATTGGAAAAAGGGAA GGAAG-3'
	3'-TCATA AGGGGAGGAGTTGTGCCG   AGGAGGTCAGGTTTATTCGTG    TCCTAACCTTTTTCCCTT CCTTC-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 006-021607069
Forward Primer: 5'-CGAATTTTGGCACTCAGGCCTAG-3' T_m: 59.148
Reverse Primer: 5'-GCAGACACCCCACTCTCTTATG-3' T_m: 59.258
Expected PCR Product Length: 318
Expected CelI Cleavage Product Sizes: 157, 161
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr6:21607196-21607252

Rank: 99	Homology Score: 2	RVD Match Score: 3.58	Left Half-Site RVD Score: 6.107	Right Half-Site RVD Score: 8.181
	Genomic Region: Intron	Closest Gene: EXOC6B
Match Type: R23L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-AGAGT TCCCCTCCCCACCATAAG  GTGGGCAAAACACATGGCCAAAT   AAAAAAGGTGCCAGAAAA AGGCC-3'
	3'-TCTCA AGGGGAGGGGTGGTATTC  CACCCGTTTTGTGTACCGGTTTA   TTTTTTCCACGGTCTTTT TCCGG-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 002-072909053
Forward Primer: 5'-CCCTTCTCTCCACTAGCCAAGAAA-3' T_m: 59.038
Reverse Primer: 5'-CTTAAGAAGAATGAGGAAATGGTTGGAGCC-3' T_m: 60.058
Expected PCR Product Length: 351
Expected CelI Cleavage Product Sizes: 220, 131
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr2:72909242-72909300

Rank: 100	Homology Score: 2	RVD Match Score: 3.58	Left Half-Site RVD Score: 7.425	Right Half-Site RVD Score: 6.717
	Genomic Region: Intron	Closest Gene: TYW1B
Match Type: R25L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-GGACT TTCACTCCACCTAGCAGT AACAAGGCACCTCTCCCCCATACAC  TCGAGTGGTGTCAGAAGA AGCTT-3'
	3'-CCTGA AAGTGAGGTGGATCGTCA TTGTTCCGTGGAGAGGGGGTATGTG  AGCTCACCACAGTCTTCT TCGAA-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 007-072071381
Forward Primer: 5'-ACTCCTACTACCAGGGCGGTAT-3' T_m: 59.258
Reverse Primer: 5'-TTGTGACTGTTCCCCCCTTAGAC-3' T_m: 59.148
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 135, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr7:72071484-72071544

Plate format Oligo Order Form
Well Name Sequence 5' mods Internal Notes
Primer Plate #1
A1 019-055627003F CCAGGAAATGGGGGTGTGTC Hockemeyer_AAVS1_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
A2 019-055627003R GTCCACTTCAGGACAGCATGTTTG Hockemeyer_AAVS1_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
B1 007-115754830F CGGTTGAGACTGAAGCATGCAAAG Hockemeyer_AAVS1_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
B2 007-115754830R AAGGACCACACTACTGACATTAGATCTACC Hockemeyer_AAVS1_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
C1 001-024697363F AGCCTCCCAAAGTGCTAGGAC Hockemeyer_AAVS1_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
C2 001-024697363R GTGGAGAACATCCATTCACAGGG Hockemeyer_AAVS1_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
D1 00X-031479613F GTCTGGACATGTCTGAATGCCTAG Hockemeyer_AAVS1_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
D2 00X-031479613R GGTTAGGTAAAGTGTCCCCTGC Hockemeyer_AAVS1_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
E1 017-035084330F GAGCCTCTGCTGGTTGGATG Hockemeyer_AAVS1_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
E2 017-035084330R ATGTTGGGTGCTGGGGAAGG Hockemeyer_AAVS1_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
F1 013-103511881F TCTCTGGCTGGCAGTTGAGG Hockemeyer_AAVS1_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
F2 013-103511881R CAGGTCACTGAAAACTGCAAACCG Hockemeyer_AAVS1_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
G1 003-124702567F TGCAAGTCTACAACCAGGGTGTG Hockemeyer_AAVS1_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
G2 003-124702567R CAGAAGGCAGCTGGAAGTCTC Hockemeyer_AAVS1_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
H1 011-011075984F GCCTCATTACTGCTAGGTGGAAG Hockemeyer_AAVS1_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
H2 011-011075984R CACCCTAACATCCAGGCATGATGA Hockemeyer_AAVS1_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
A3 011-119574363F CAACGAGGCTGACGCTCTTTC Hockemeyer_AAVS1_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
A4 011-119574363R CTGAGCACCTGTGTGCCAGT Hockemeyer_AAVS1_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
B3 006-011853744F GTCTACGGATCTAACACTCCAAAAATCTTAGC Hockemeyer_AAVS1_Ranking-RVDs_5T	Warning! Primer pair was generated using suboptimal parameters. 
B4 006-011853744R CTAGGGGGCTGAAATGGAACAAC Hockemeyer_AAVS1_Ranking-RVDs_5T	Warning! Primer pair was generated using suboptimal parameters. 
C3 004-148942680F GTAGGTAAGCCAGGGAGCTGT Hockemeyer_AAVS1_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
C4 004-148942680R CCTGCCAACACCATGTTTCCAC Hockemeyer_AAVS1_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
D3 012-030180063F CTATAAGGCTGCTGGGCCTTTC Hockemeyer_AAVS1_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
D4 012-030180063R GAGGCAAACCTACTTTATGGAGGG Hockemeyer_AAVS1_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
E3 008-145493530F GAGCCATTAAGGGCTAGAGGG Hockemeyer_AAVS1_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
E4 008-145493530R GGAAAGCTCCCAAGAAGGGAAG Hockemeyer_AAVS1_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
F3 008-145324445F GAGCCATTAAGGGCTAGAGGG Hockemeyer_AAVS1_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
F4 008-145324445R GGAAAGCTCCCAAGAAGGGAAG Hockemeyer_AAVS1_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
G3 020-058618528F GGGAGCTATGATCATGCCATAGG Hockemeyer_AAVS1_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
G4 020-058618528R GCTCCATGTCATTGTTCTGGGC Hockemeyer_AAVS1_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
H3 001-062633493F TCCTCCTGCCTAATGGCTTTACTG Hockemeyer_AAVS1_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
H4 001-062633493R GCTACTGAGCCCAGCCTAATTTAC Hockemeyer_AAVS1_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
A5 002-064716657F GAAATGCATCCCCTCTAGCCTG Hockemeyer_AAVS1_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
A6 002-064716657R GCTGCATGGACAGTGCTCACA Hockemeyer_AAVS1_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
B5 021-036773953F CCAGAGCGGTTTTCCAAGATGC Hockemeyer_AAVS1_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
B6 021-036773953R GGATTCACAACACCATGGATGGAC Hockemeyer_AAVS1_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
C5 005-157580667F GTAATCCAGGGTCTCTGTTTGTCC Hockemeyer_AAVS1_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
C6 005-157580667R GTCTTTGGGTGGCATTTGTGAGG Hockemeyer_AAVS1_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
D5 017-008242168F CAGCTATTGCGGGGAGCTAATG Hockemeyer_AAVS1_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
D6 017-008242168R AGGCCACAGCTCAGAACTGTGGA Hockemeyer_AAVS1_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
E5 015-067305574F CCCCACCTTGCTGAATGGAC Hockemeyer_AAVS1_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
E6 015-067305574R CCAGTCCTTGATCTCTTCCTGAGT Hockemeyer_AAVS1_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
F5 006-092651079F CAGCAAGGAGGCCAAGAACTCA Hockemeyer_AAVS1_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
F6 006-092651079R GTGTGTGGGCCTCAAGTTTTGTAC Hockemeyer_AAVS1_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
G5 003-070995805F GCCGTTGCTTTTGCCTTGAACATC Hockemeyer_AAVS1_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
G6 003-070995805R TACTTGAGTCCCTGGATCTGGC Hockemeyer_AAVS1_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
H5 002-001827851F GGGGGTTGGGGAACGTTTTTAG Hockemeyer_AAVS1_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
H6 002-001827851R TCAGGACGAGAGCAGCTCAAATG Hockemeyer_AAVS1_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
A7 010-024751913F GGTGGATGTGCAGAGATGGAG Hockemeyer_AAVS1_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
A8 010-024751913R CTCTACTGAGCCCTCCAGTG Hockemeyer_AAVS1_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
B7 004-090656661F CAGGGGAGTGTGTACATAGTGTAG Hockemeyer_AAVS1_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
B8 004-090656661R CCAGAGTTGTTCCTCTCCACATAG Hockemeyer_AAVS1_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
C7 006-021174562F GTCGGTATAATCTTAGTCAAAAGTTCAACAGGG Hockemeyer_AAVS1_Ranking-RVDs_5T	Warning! Primer pair was generated using suboptimal parameters. 
C8 006-021174562R TAGTTAAATTGCCCTCCAAAATGGCCTTATCAATG Hockemeyer_AAVS1_Ranking-RVDs_5T	Warning! Primer pair was generated using suboptimal parameters. 
D7 001-163803301F GGTTAAGTGCTTGACCACTGCTG Hockemeyer_AAVS1_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
D8 001-163803301R CACCATGGCACATGTCTACTTGAG Hockemeyer_AAVS1_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
E7 020-050029460F AGAAGCACAGGATGAGGCCG Hockemeyer_AAVS1_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
E8 020-050029460R CAGTGGATTGACAGAGGCAGATG Hockemeyer_AAVS1_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
F7 007-158192599F TAGTCCCCAGGCACTTGCAG Hockemeyer_AAVS1_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
F8 007-158192599R GCCTCGTAAGTTTAGGCATCCTC Hockemeyer_AAVS1_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
G7 002-096866458F CGCCGAAACTGGCCTCAAATTCC Hockemeyer_AAVS1_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
G8 002-096866458R CTGCCTAGCAGTTACCTTTGTGG Hockemeyer_AAVS1_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
H7 007-030310147F TTGCTGCTGCCAAAAGCTACCC Hockemeyer_AAVS1_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
H8 007-030310147R GAGCTCCAGTTTCCTGCACTGT Hockemeyer_AAVS1_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
A9 003-039683322F GGTCTCCAGGGAGCATTATACAG Hockemeyer_AAVS1_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
A10 003-039683322R CTAGACACAGAGCCTTCAGTAAGC Hockemeyer_AAVS1_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
B9 017-046139902F TGTAACCTGGTTCAGCTCAGTCC Hockemeyer_AAVS1_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
B10 017-046139902R ACCGCACCAGGCCTCAAACT Hockemeyer_AAVS1_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
C9 012-025903897F CCAGCATCTTGTGGCTGAGTAG Hockemeyer_AAVS1_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
C10 012-025903897R GGGAAGATGACAGTGTTCTTGACAGG Hockemeyer_AAVS1_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
D9 008-108496180F GATGGGAAAGATGCCTAGGAAGC Hockemeyer_AAVS1_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
D10 008-108496180R GGAAACTGAGGGCCACTAAAGTC Hockemeyer_AAVS1_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
E9 004-088055877F TGGGGTGTTTGGATTGGGAGG Hockemeyer_AAVS1_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
E10 004-088055877R TCAGCTAGCATTTCAGCTCAGGC Hockemeyer_AAVS1_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
F9 010-096170810F GGCATGCCAGAGATAGGACTATTC Hockemeyer_AAVS1_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
F10 010-096170810R GTGCTACAGTGGCAACCTTCATG Hockemeyer_AAVS1_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
G9 011-126543310F CCAGCAGCTCTGGAAATTGGATC Hockemeyer_AAVS1_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
G10 011-126543310R GAGGAAGATCTTACAGACCCAACC Hockemeyer_AAVS1_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
H9 004-115491385F CATGTTGCACAGGCTGAGTACTCT Hockemeyer_AAVS1_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
H10 004-115491385R CCCATAGCTCTTTGGTCAGGTC Hockemeyer_AAVS1_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
A11 00X-132539743F CTGCCATAGACTGGGAAACTGGA Hockemeyer_AAVS1_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
A12 00X-132539743R CCAGAACACAGCTGGGAATGTGGA Hockemeyer_AAVS1_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
B11 011-008291463F GTTTCCTCGGGCAATGAATGAGC Hockemeyer_AAVS1_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
B12 011-008291463R GCACAGTCCTCCTTTCTTCACC Hockemeyer_AAVS1_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
C11 002-149982161F GGCATCTTCGAGTACCTTAACTCC Hockemeyer_AAVS1_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
C12 002-149982161R CTCTTCTCAAGTTCAAGGGTCAGTTGTAC Hockemeyer_AAVS1_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
D11 007-052309823F CCTGAGTCCCTGCTGCATTAATTC Hockemeyer_AAVS1_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
D12 007-052309823R ACTCTGTGTCCTCAACGGTGTTC Hockemeyer_AAVS1_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
E11 010-093286041F GATTCTGAGCAGGGAGCTTGG Hockemeyer_AAVS1_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
E12 010-093286041R ACAGGATGAGCTAGGGCCTGA Hockemeyer_AAVS1_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
F11 016-081100645F GCCCCCAAAGTGCATGTTTGGA Hockemeyer_AAVS1_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
F12 016-081100645R GAGCTCTCCGATAACATCATGGC Hockemeyer_AAVS1_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
G11 006-021198081F CCGTGATTTGAACAGGGTTCGC Hockemeyer_AAVS1_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
G12 006-021198081R CTCTCCCCTTTAAATGCCCTTTGC Hockemeyer_AAVS1_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
H11 007-030310150F CTGCTGCCAAAAGCTACCCATG Hockemeyer_AAVS1_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
H12 007-030310150R GAGCTCCAGTTTCCTGCACTGT Hockemeyer_AAVS1_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
Primer Plate #2
A1 004-085429094F GTCTCCCTTGCCAGACTATAACC Hockemeyer_AAVS1_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
A2 004-085429094R CATGGCCAAGAGCTGTGCTTTC Hockemeyer_AAVS1_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
B1 001-198360906F GGTTTTATAGCCCTTCTCCTGCC Hockemeyer_AAVS1_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
B2 001-198360906R GTTGTGGGATTTGAGCTGGTGG Hockemeyer_AAVS1_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
C1 007-140355184F CCCACAAGGGCCTTATTTTCAGTC Hockemeyer_AAVS1_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
C2 007-140355184R GAGAGCTGATTTCACCCCTCTAG Hockemeyer_AAVS1_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
D1 00X-013108270F GCCATTTTGGCAGAGCCACTGA Hockemeyer_AAVS1_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
D2 00X-013108270R GCATGTTGTGCACGAAATGACCTC Hockemeyer_AAVS1_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
E1 002-037018108F TTTCAGCTGCATCCCGGACC Hockemeyer_AAVS1_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
E2 002-037018108R AGGAGATGCGGCCAAGTCAG Hockemeyer_AAVS1_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
F1 004-089270564F GTGGTTGGGGCACAGCTTAG Hockemeyer_AAVS1_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
F2 004-089270564R CACACCTGCCTCCCATTCTATTC Hockemeyer_AAVS1_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
G1 017-044029096F GGACGGACTCAGGAGGCAAT Hockemeyer_AAVS1_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
G2 017-044029096R CACACCAAGGCTGTTTTCCTCAGA Hockemeyer_AAVS1_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
H1 ap1-000838311F ACCAAGGCTGTTTTCCTCAGACC Hockemeyer_AAVS1_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
H2 ap1-000838311R AACCACAGGGACGGACTCAG Hockemeyer_AAVS1_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
A3 003-135693383F CTGTGCTTGCTTTGTCCCAGTC Hockemeyer_AAVS1_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
A4 003-135693383R CCTTGCTAGGATTCCTAAGCCC Hockemeyer_AAVS1_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
B3 012-071464141F CCACAAGTTTGGGGAGCAAGAC Hockemeyer_AAVS1_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
B4 012-071464141R AAGCAAATGCCTAGTGTGGGAAGC Hockemeyer_AAVS1_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
C3 001-024882812F CATCTGTTGGAGGAAGTGCCTG Hockemeyer_AAVS1_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
C4 001-024882812R CTCTGCATAGGGCAGACCCA Hockemeyer_AAVS1_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
D3 004-027261444F GTGGGTTAAGAGAGGTATTTGGCC Hockemeyer_AAVS1_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
D4 004-027261444R CCTATCCAAGCCACGATTAACTCC Hockemeyer_AAVS1_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
E3 009-138652006F GGGAAGGGTCAAGGTGGACA Hockemeyer_AAVS1_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
E4 009-138652006R AATAGCAGGGTCCCTCCAGGGT Hockemeyer_AAVS1_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
F3 015-033799697F GGCACTGTGGCCAATTTTATGGCT Hockemeyer_AAVS1_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
F4 015-033799697R CCCAGTCCTACGAGATAGATTGC Hockemeyer_AAVS1_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
G3 00X-096909115F TAGAGAATGGTGTTTGGGGCTGC Hockemeyer_AAVS1_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
G4 00X-096909115R GGCTTGTATCTCAAAACTCCCACAGATAAC Hockemeyer_AAVS1_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
H3 006-014315745F GGAAGGAAGGAAGGAAGGAGAAAG Hockemeyer_AAVS1_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
H4 006-014315745R CCCTGCTTGTTTTTAAGGGGCC Hockemeyer_AAVS1_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
A5 017-031174782F CTCAGCCTCCCAAAATGCTGAGA Hockemeyer_AAVS1_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
A6 017-031174782R CGGAGAGAGGCCCATTTTTCATAG Hockemeyer_AAVS1_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
B5 012-003414995F TGACGTTCCCTGGGCCAAGA Hockemeyer_AAVS1_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
B6 012-003414995R AAAATCCAGTTCCTTGCTCGCACC Hockemeyer_AAVS1_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
C5 014-026411302F CCAGTCCCAAGGATTCCAGTTTAG Hockemeyer_AAVS1_Ranking-RVDs_5T	Warning! Primer pair was generated using suboptimal parameters. 
C6 014-026411302R TTCCAAATAAAATGATCTCTCCTTCCTTTGAATCTGGC Hockemeyer_AAVS1_Ranking-RVDs_5T	Warning! Primer pair was generated using suboptimal parameters. 
D5 002-097793909F GGTTGGAGATGCAGACGTAGAATG Hockemeyer_AAVS1_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
D6 002-097793909R GAAAAGGGTACCGCCAAAGAGC Hockemeyer_AAVS1_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
E5 001-226575130F GGAGACAAAAATGCCAGCCCTATC Hockemeyer_AAVS1_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
E6 001-226575130R CCACTGTGGCACATCTGTGTC Hockemeyer_AAVS1_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
F5 007-045162226F GGGGAGGCTGGTGATTTTTTTGAG Hockemeyer_AAVS1_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
F6 007-045162226R GCTGAACACGGTATACAAGGCAAC Hockemeyer_AAVS1_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
G5 011-060790178F GCCTGTGCTGAGACTGGGAA Hockemeyer_AAVS1_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
G6 011-060790178R AGCTGTCAGTCAAATTGCTGGGTG Hockemeyer_AAVS1_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
H5 007-005466629F AAATGTAAAAACCAGGCCTGGGCG Hockemeyer_AAVS1_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
H6 007-005466629R CCCCAGCCGATTTTCTACCC Hockemeyer_AAVS1_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
A7 015-071318700F GTGCTGCAATAAACATACATGTGCATGTGTC Hockemeyer_AAVS1_Ranking-RVDs_5T	Warning! Primer pair was generated using suboptimal parameters. 
A8 015-071318700R CCCCATCAAAAAGTGGGTGAAGG Hockemeyer_AAVS1_Ranking-RVDs_5T	Warning! Primer pair was generated using suboptimal parameters. 
B7 008-129090046F CATCCTCCCAGCCATCAATACC Hockemeyer_AAVS1_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
B8 008-129090046R AGGCTGGGACCACAGGCATA Hockemeyer_AAVS1_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
C7 00Y-028570220F CTGAGGTTGGAGATGCAGAAGTAG Hockemeyer_AAVS1_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
C8 00Y-028570220R GAAAACGGTACCGCCAAAGAGC Hockemeyer_AAVS1_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
D7 002-103259635F GTTCTCTGCGTTCTTTACGTCGTC Hockemeyer_AAVS1_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
D8 002-103259635R GTCAACCTCACAAGGCACAGAAG Hockemeyer_AAVS1_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
E7 003-109619335F CAGGGAAGTCTCAGTGGTTTTTGG Hockemeyer_AAVS1_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
E8 003-109619335R CCAGGTGTATTCTGTGTGGCTAG Hockemeyer_AAVS1_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
F7 012-129962958F CCACCATCTCATGTATGGTTGAGC Hockemeyer_AAVS1_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
F8 012-129962958R GGGGATCAGAGCACATTCCAG Hockemeyer_AAVS1_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
G7 010-003936310F GGGTGATGTGGTTTTCAGCTGTTG Hockemeyer_AAVS1_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
G8 010-003936310R TTCGCTAGGAATGGCCTCTACG Hockemeyer_AAVS1_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
H7 007-066670329F TGACTGTTCCCCCCTTAGACTC Hockemeyer_AAVS1_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
H8 007-066670329R TTGACTCCTACTACCAGGGCG Hockemeyer_AAVS1_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
A9 004-048328999F AAATCTCTTCCACCCCACCACTC Hockemeyer_AAVS1_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
A10 004-048328999R TACAGGATAGGGGGGTGTGG Hockemeyer_AAVS1_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
B9 014-096450488F GCTGCCCAAGCTCTTACAATGATC Hockemeyer_AAVS1_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
B10 014-096450488R GTAAGCACATGGTCTGCTTCCG Hockemeyer_AAVS1_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
C9 012-011342495F CTGTCTTCCAGTCTAAAGCAACTACCTC Hockemeyer_AAVS1_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
C10 012-011342495R CCACACATCCTCTTCCTCCTATTC Hockemeyer_AAVS1_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
D9 015-093472933F GGTGTGGATCCCGTTGACAATCT Hockemeyer_AAVS1_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
D10 015-093472933R GCCAAGTGCAGGTTTGCTTACAG Hockemeyer_AAVS1_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
E9 004-084304603F CCTACCTCTCCTCTTCAATTCCTC Hockemeyer_AAVS1_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
E10 004-084304603R GCCTAGCTTCCAGAATCTGAACAC Hockemeyer_AAVS1_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
F9 00X-068408586F GCACTCCTTTACCCTCCGATATC Hockemeyer_AAVS1_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
F10 00X-068408586R AGGTTTGAGGTGCACTACTTCAGG Hockemeyer_AAVS1_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
G9 006-000344885F ACCTTCCTCACCTGCGTCAC Hockemeyer_AAVS1_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
G10 006-000344885R AACCTGGTCATACTGTCTGTGGC Hockemeyer_AAVS1_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
H9 006-136482738F GGGAGGGAACCCATTGCAGT Hockemeyer_AAVS1_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
H10 006-136482738R CTATTGAGGGCAGGGAGCGT Hockemeyer_AAVS1_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
A11 003-056061208F GGCATGGATATCCAGGCTTGCT Hockemeyer_AAVS1_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
A12 003-056061208R TGGGGATGTACAGAGGCCAG Hockemeyer_AAVS1_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
B11 005-054696084F CGGCATCCCCTTATTAGACCC Hockemeyer_AAVS1_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
B12 005-054696084R CTGACAGGTTCAGAGCTGACAGT Hockemeyer_AAVS1_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
C11 021-046316063F GATCCTGCCTGGTGGATTTCAAG Hockemeyer_AAVS1_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
C12 021-046316063R ATGTGTGAGCATGGGGGAGG Hockemeyer_AAVS1_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
D11 016-033560648F GTTCTTTTGAAAAGGGTACTGCCAAAGAGC Hockemeyer_AAVS1_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
D12 016-033560648R GGTTGGAGATGCAGACGTAGAATG Hockemeyer_AAVS1_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
E11 00X-147453463F GCCACCCACAGATCATTTCAAAGG Hockemeyer_AAVS1_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
E12 00X-147453463R GGAAAGCTTTTCCCATGTGGACAG Hockemeyer_AAVS1_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
F11 001-185833320F CATCGTGTAAGAGAAGGCAGAGTG Hockemeyer_AAVS1_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
F12 001-185833320R CTGTTGGATAAGTTGCAGCACCTC Hockemeyer_AAVS1_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
G11 010-038264363F GGTGCTTACGAAATGCCTAAGCTC Hockemeyer_AAVS1_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
G12 010-038264363R AGGCCTTCCTGCATCCTCCA Hockemeyer_AAVS1_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
H11 007-039538608F GCAGGGATGAATGTGATTCCACAG Hockemeyer_AAVS1_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
H12 007-039538608R GAGATTGTGCTACTGCCCTCC Hockemeyer_AAVS1_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
Primer Plate #3
A1 010-014726614F GCATTGTGCCAAAAGGATCCCG Hockemeyer_AAVS1_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
A2 010-014726614R GATAGGGAGACCCCATGTCTAC Hockemeyer_AAVS1_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
B1 006-021607069F CGAATTTTGGCACTCAGGCCTAG Hockemeyer_AAVS1_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
B2 006-021607069R GCAGACACCCCACTCTCTTATG Hockemeyer_AAVS1_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
C1 002-072909053F CCCTTCTCTCCACTAGCCAAGAAA Hockemeyer_AAVS1_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
C2 002-072909053R CTTAAGAAGAATGAGGAAATGGTTGGAGCC Hockemeyer_AAVS1_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
D1 007-072071381F ACTCCTACTACCAGGGCGGTAT Hockemeyer_AAVS1_Ranking-RVDs_5T	Primer pair generated using optimal parameters. 
D2 007-072071381R TTGTGACTGTTCCCCCCTTAGAC Hockemeyer_AAVS1_Ranking-RVDs_5T	Primer pair generated using optimal parameters.
157 total sites were located
Mismatch Types	Genomic Regions
0 and 0: 1 2 and 5: 1 3 and 5: 4 4 and 4: 1 4 and 5: 33 5 and 5: 117	Exons: 6 Promoters: 1 Introns: 70 Intergenic: 80