PROGNOS Summary for Hockemeyer_AAVS1_Ranking-RVDs

The hg19 genome was searched for the TALEN pair targeting:
5'- TTTTCTGTCACCAATCCT NN..NN ACTGTGGGGTGGAGGGGA -3'

Using these RVDs:
Left:  01NG02NG03NG04HD05NG06NN07NG08HD09NI10HD11HD12NI13NI14NG15HD16HD17NG
Right: 01HD02HD03HD04HD05NG06HD07HD08NI09HD10HD11HD12HD13NI14HD15NI16NN17NG

Up to 5 mismatches were allowed in each nuclease half-site.

Allowed spacing distances were: 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26

Only heterodimers of the nuclease pair were included in the output

292 total sites were located
Mismatch Types
Genomic Regions
  0 and 0: 1
  2 and 5: 1
  3 and 5: 5
  4 and 4: 2
  4 and 5: 60
  5 and 5: 223
  Exons: 10
  Promoters: 5
  Introns: 130
  Intergenic: 147


The top 100 sites are ranked below according to a weighting of the RVD base preferences.
For the full output, click here.

This output will be deleted from the server one week after it is generated.  Please save your results.

Primer pairs were designed using the 400 bp of sequence on either side of the cleavage site to melt close to 60 °C

For easy visualization of cleavage on gels:
The smallest fragment will be longer than 130 bp, which is also the minimum separation distance between uncleaved and cleaved products.
The two cleavage products will be separated by a minimum size of 0 bp
To optimize assay conditions, the amplicons have a minimum length of 260 bp, a maximum length of 450 bp, and an optimal length of 270 bp.
Rank: 1	Homology Score: 72	RVD Match Score: 2	Left Half-Site RVD Score: 2.343	Right Half-Site RVD Score: 2.083
	Genomic Region: Intron	Closest Gene: PPP1R12C	Caution! This site scores very closely to the intended nuclease target. The potential for cleavage at this site is of great concern.
Match Type: L15R
	           Left Nuclease
	         TTTTCTGTCACCAATCCT
	5'-GGGGC TTTTCTGTCACCAATCCT      GTCCCTAGTGGCCCC       ACTGTGGGGTGGAGGGGA CAGAT-3'
	3'-CCCCG AAAAGACAGTGGTTAGGA      CAGGGATCACCGGGG       TGACACCCCACCTCCCCT GTCTA-5'
	                                                       TGACACCCCACCTCCCCT
	                                                            Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 019-055627003
Forward Primer: 5'-CCAGGAAATGGGGGTGTGTC-3' Tm: 59.508
Reverse Primer: 5'-GTCCACTTCAGGACAGCATGTTTG-3' Tm: 59.038
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 131, 139
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr19:55627107-55627157

Rank: 2	Homology Score: 5	RVD Match Score: 3.027	Left Half-Site RVD Score: 4.567	Right Half-Site RVD Score: 5.609
	Genomic Region: Exon	Closest Gene: CACNG3
Match Type: R26L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-ACTCT CCCCCTCCAACCCCCAGC CGTCCAGAGTACCATGAAGAATTATG AGGATGTGTGACAGAGGT ATCCA-3'
	3'-TGAGA GGGGGAGGTTGGGGGTCG GCAGGTCTCATGGTACTTCTTAATAC TCCTACACACTGTCTCCA TAGGT-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 016-024267933
Forward Primer: 5'-CAAAGTCCCTGTGGATGCTGACAA-3' Tm: 59.038
Reverse Primer: 5'-TAGTCCTGCACACACCTCTGG-3' Tm: 59.378
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 135, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr16:24268035-24268096

Rank: 3	Homology Score: 5	RVD Match Score: 3.08	Left Half-Site RVD Score: 3.962	Right Half-Site RVD Score: 6.783
	Genomic Region: Intergenic	Closest Gene: C11orf87
Match Type: R26L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-ACAAC AACACTCCACCCCACAAT TCTCTGCTGAGTGTCTTGGCTACAAG GTGGTTTGTGACAGCAAA ACTCC-3'
	3'-TGTTG TTGTGAGGTGGGGTGTTA AGAGACGACTCACAGAACCGATGTTC CACCAAACACTGTCGTTT TGAGG-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 011-109627678
Forward Primer: 5'-TCCTCCTTGGAACCCCAGATTTG-3' Tm: 59.148
Reverse Primer: 5'-TGTCTTCACTGCTGCACATTCCC-3' Tm: 59.148
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 135, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr11:109627780-109627841

Rank: 4	Homology Score: 5	RVD Match Score: 3.145	Left Half-Site RVD Score: 5.991	Right Half-Site RVD Score: 4.981
	Genomic Region: Intron	Closest Gene: TEX264
Match Type: L24R
	           Left Nuclease
	         TTTTCTGTCACCAATCCT
	5'-TTCCC TTTTTTGCCAACACTCCT  GAGTGAGTAAGCAGGTGTTGGTGC  AGGGTGGGGTGGTGGTGG GGAAG-3'
	3'-AAGGG AAAAAACGGTTGTGAGGA  CTCACTCATTCGTCCACAACCACG  TCCCACCCCACCACCACC CCTTC-5'
	                                                       TGACACCCCACCTCCCCT
	                                                            Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 003-051729704
Forward Primer: 5'-GGAGATGAACTCAGGCTGAAGTG-3' Tm: 59.148
Reverse Primer: 5'-TTCCCTCCCACCTGGGTCTT-3' Tm: 59.508
Expected PCR Product Length: 271
Expected CelI Cleavage Product Sizes: 141, 130
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr3:51729813-51729872

Rank: 5	Homology Score: 17	RVD Match Score: 3.15	Left Half-Site RVD Score: 3.963	Right Half-Site RVD Score: 7.356
	Genomic Region: Intergenic	Closest Gene: TFEC
Match Type: R11L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-CCCCC TCCCCGCCACCCCCCAGT        AATGGGGAGAG         AGGACATTTGACAGAGAA TGAAG-3'
	3'-GGGGG AGGGGCGGTGGGGGGTCA        TTACCCCTCTC         TCCTGTAAACTGTCTCTT ACTTC-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 007-115754830
Forward Primer: 5'-CGGTTGAGACTGAAGCATGCAAAG-3' Tm: 59.038
Reverse Primer: 5'-AAGGACCACACTACTGACATTAGATCTACC-3' Tm: 60.058
Expected PCR Product Length: 274
Expected CelI Cleavage Product Sizes: 143, 131
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr7:115754948-115754994

Rank: 6	Homology Score: 2	RVD Match Score: 3.158	Left Half-Site RVD Score: 5.88	Right Half-Site RVD Score: 5.124
	Genomic Region: Exon	Closest Gene: KIAA1244
Match Type: R26L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-CTGCT GCCCCCCAGCCCCAAAGT GGAGAAGAAGGATCCCAGCCGGAAGA AGGAGTGGTGGGAGAATG CGGGG-3'
	3'-GACGA CGGGGGGTCGGGGTTTCA CCTCTTCTTCCTAGGGTCGGCCTTCT TCCTCACCACCCTCTTAC GCCCC-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 006-138655843
Forward Primer: 5'-GGCTTCTCTGGGAAAGAAACCCCT-3' Tm: 60.908
Reverse Primer: 5'-TTTGGGGAACGCGGACAGGT-3' Tm: 59.508
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 137, 133
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr6:138655947-138656008

Rank: 7	Homology Score: 8	RVD Match Score: 3.159	Left Half-Site RVD Score: 4.945	Right Half-Site RVD Score: 6.14
	Genomic Region: Intron	Closest Gene: NINL
Match Type: R23L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-GAGAC ACACCTGCACCCCACATT  TTCCAGGCCCTGGAAGGCAGCTT   ATGGTTTGAGACAGAAAA GGAGG-3'
	3'-CTCTG TGTGGACGTGGGGTGTAA  AAGGTCCGGGACCTTCCGTCGAA   TACCAAACTCTGTCTTTT CCTCC-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 020-025439956
Forward Primer: 5'-AGTCCCTGCACTGACTCAGC-3' Tm: 59.508
Reverse Primer: 5'-GTGCTTGATGCGTGGGAGTC-3' Tm: 59.508
Expected PCR Product Length: 285
Expected CelI Cleavage Product Sizes: 134, 151
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr20:25440059-25440117

Rank: 8	Homology Score: 10	RVD Match Score: 3.161	Left Half-Site RVD Score: 3.845	Right Half-Site RVD Score: 7.621
	Genomic Region: Intron	Closest Gene: STPG1
Match Type: R11L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-ACACT TCCCCACCACACCACAAT        GCCCTGTCTCA         AGAAACTGTGACAGAATA AAATG-3'
	3'-TGTGA AGGGGTGGTGTGGTGTTA        CGGGACAGAGT         TCTTTGACACTGTCTTAT TTTAC-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 001-024697363
Forward Primer: 5'-AGCCTCCCAAAGTGCTAGGAC-3' Tm: 59.378
Reverse Primer: 5'-GTGGAGAACATCCATTCACAGGG-3' Tm: 59.148
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 135, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr1:24697473-24697519

Rank: 9	Homology Score: 10	RVD Match Score: 3.161	Left Half-Site RVD Score: 5.309	Right Half-Site RVD Score: 5.744
	Genomic Region: Intergenic	Closest Gene: MIR548X
Match Type: R21L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-AAATC TTCTCTCCACACCACAGT   GCAGCTGCTGGGGGTTGGGAG    AGGAGTGGTGTAAGCAAT TTGAG-3'
	3'-TTTAG AAGAGAGGTGTGGTGTCA   CGTCGACGACCCCCAACCCTC    TCCTCACCACATTCGTTA AACTC-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 016-009446101
Forward Primer: 5'-ATCCTTCCTCTGGGGGCACT-3' Tm: 59.508
Reverse Primer: 5'-CTCCAACCTCCCAATGAAACACC-3' Tm: 59.148
Expected PCR Product Length: 334
Expected CelI Cleavage Product Sizes: 155, 179
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr16:9446226-9446282

Rank: 10	Homology Score: 5	RVD Match Score: 3.179	Left Half-Site RVD Score: 5.326	Right Half-Site RVD Score: 5.853
	Genomic Region: Intergenic	Closest Gene: AMIGO2
Match Type: R11L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-GGATC CTCCCACCACCCCACGAT        GTAAAACTTGA         AGGGTTGATGGCTGAAAA AATAT-3'
	3'-CCTAG GAGGGTGGTGGGGTGCTA        CATTTTGAACT         TCCCAACTACCGACTTTT TTATA-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 012-047346370
Forward Primer: 5'-CTTGGTATGTTCCAGTGTGGCC-3' Tm: 59.258
Reverse Primer: 5'-GGATGTGTGCAGGATGTACAGG-3' Tm: 59.258
Expected PCR Product Length: 272
Expected CelI Cleavage Product Sizes: 134, 138
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr12:47346479-47346525

Rank: 11	Homology Score: 5	RVD Match Score: 3.195	Left Half-Site RVD Score: 6.316	Right Half-Site RVD Score: 5.03
	Genomic Region: Intron	Closest Gene: DMD
Match Type: L23R
	           Left Nuclease
	         TTTTCTGTCACCAATCCT
	5'-TCTTC TCTTCCATCACTAATTCT  GATGAACAGCTATCAAGCTATTC   ATGGTAGGGTGGAGGGTA GGTGG-3'
	3'-AGAAG AGAAGGTAGTGATTAAGA  CTACTTGTCGATAGTTCGATAAG   TACCATCCCACCTCCCAT CCACC-5'
	                                                       TGACACCCCACCTCCCCT
	                                                            Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 00X-031479613
Forward Primer: 5'-GTCTGGACATGTCTGAATGCCTAG-3' Tm: 59.038
Reverse Primer: 5'-GGTTAGGTAAAGTGTCCCCTGC-3' Tm: 59.258
Expected PCR Product Length: 339
Expected CelI Cleavage Product Sizes: 156, 183
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chrX:31479738-31479796

Rank: 12	Homology Score: 5	RVD Match Score: 3.199	Left Half-Site RVD Score: 5.956	Right Half-Site RVD Score: 5.371
	Genomic Region: Intergenic	Closest Gene: C3orf80
Match Type: L22R
	           Left Nuclease
	         TTTTCTGTCACCAATCCT
	5'-TGCCC ACTTCTGTCACCCCTGCT   AGACCACTCTGGGTTGTGGGGG   ACTGTAGGGTGCATGGGC CTCAG-3'
	3'-ACGGG TGAAGACAGTGGGGACGA   TCTGGTGAGACCCAACACCCCC   TGACATCCCACGTACCCG GAGTC-5'
	                                                       TGACACCCCACCTCCCCT
	                                                            Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 003-159874261
Forward Primer: 5'-GCCCAGCTCTGAAAAGGACTAAG-3' Tm: 59.148
Reverse Primer: 5'-GCTCCACTGAATATCCCCCAAG-3' Tm: 59.258
Expected PCR Product Length: 284
Expected CelI Cleavage Product Sizes: 146, 138
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr3:159874376-159874433

Rank: 13	Homology Score: 2	RVD Match Score: 3.208	Left Half-Site RVD Score: 4.892	Right Half-Site RVD Score: 6.583
	Genomic Region: Intergenic	Closest Gene: SUCNR1
Match Type: R24L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-GGGAA CTCCCACCACCCCACAAA  GGGGGAATTACTCTTTTCTTCCAT  CAGAATGGTGGCAGAAAC CTGTG-3'
	3'-CCCTT GAGGGTGGTGGGGTGTTT  CCCCCTTAATGAGAAAAGAAGGTA  GTCTTACCACCGTCTTTG GACAC-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 003-151589613
Forward Primer: 5'-GGCTACAGTCTAGATGACGTGTTC-3' Tm: 59.038
Reverse Primer: 5'-CGACTCTGATTCTTGACTGCCTAC-3' Tm: 59.038
Expected PCR Product Length: 440
Expected CelI Cleavage Product Sizes: 152, 288
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr3:151589733-151589792

Rank: 14	Homology Score: 5	RVD Match Score: 3.21	Left Half-Site RVD Score: 5.102	Right Half-Site RVD Score: 6.27
	Genomic Region: Intron	Closest Gene: KLF7
Match Type: L20R
	           Left Nuclease
	         TTTTCTGTCACCAATCCT
	5'-TCATC ATGTCTGTCACCAAACAT    GACAAATATTTACCCTTCCA    ACTGTATGGTGGACTGGT TCTAA-3'
	3'-AGTAG TACAGACAGTGGTTTGTA    CTGTTTATAAATGGGAAGGT    TGACATACCACCTGACCA AGATT-5'
	                                                       TGACACCCCACCTCCCCT
	                                                            Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 002-207954053
Forward Primer: 5'-GTTTTAGCCACAGGGAAGCTGC-3' Tm: 59.258
Reverse Primer: 5'-GAAGCTGATCCCAAGAAGTTTGGC-3' Tm: 59.038
Expected PCR Product Length: 327
Expected CelI Cleavage Product Sizes: 171, 156
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr2:207954194-207954249

Rank: 15	Homology Score: 5	RVD Match Score: 3.215	Left Half-Site RVD Score: 6.511	Right Half-Site RVD Score: 4.912
	Genomic Region: Intergenic	Closest Gene: CDKL5
Match Type: R13L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-CCCTC TCCCCTCTACCTCCCTAT       GGTCAGAGAAGTT        AGGCTTGGTGAGAGAACT GACCA-3'
	3'-GGGAG AGGGGAGATGGAGGGATA       CCAGTCTCTTCAA        TCCGAACCACTCTCTTGA CTGGT-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 00X-018438373
Forward Primer: 5'-CAGGTCATGACCATCAGTGATCAG-3' Tm: 59.038
Reverse Primer: 5'-CACCTGTCAGGCTTGTGGCT-3' Tm: 59.508
Expected PCR Product Length: 278
Expected CelI Cleavage Product Sizes: 147, 131
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chrX:18438494-18438542

Rank: 16	Homology Score: 2	RVD Match Score: 3.224	Left Half-Site RVD Score: 6.495	Right Half-Site RVD Score: 5.07
	Genomic Region: Intron	Closest Gene: TRPM3
Match Type: L16R
	           Left Nuclease
	         TTTTCTGTCACCAATCCT
	5'-TCCTC CTTTCTGTCCAGAATCGT      TGGTTAGTTTTTGAAG      GTTGTGCGGTGGAGGTGG TTGGG-3'
	3'-AGGAG GAAAGACAGGTCTTAGCA      ACCAATCAAAAACTTC      CAACACGCCACCTCCACC AACCC-5'
	                                                       TGACACCCCACCTCCCCT
	                                                            Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 009-073729326
Forward Primer: 5'-CCACATGCTCAAGAGCCAGG-3' Tm: 59.508
Reverse Primer: 5'-GGCATGTCCCTTAGATTCCAAGGT-3' Tm: 59.038
Expected PCR Product Length: 330
Expected CelI Cleavage Product Sizes: 186, 144
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr9:73729484-73729535

Rank: 17	Homology Score: 2	RVD Match Score: 3.226	Left Half-Site RVD Score: 5.002	Right Half-Site RVD Score: 6.591
	Genomic Region: Intergenic	Closest Gene: RTN4RL2
Match Type: R13L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-TCGTC CCCCCTCCCCCCAACCCT       AACCGGAGCTAGG        TGGGGTGGGGACAGCAAA TGTGG-3'
	3'-AGCAG GGGGGAGGGGGGTTGGGA       TTGGCCTCGATCC        ACCCCACCCCTGTCGTTT ACACC-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 011-057225300
Forward Primer: 5'-AGCCTCACCGTACAGATGAGGA-3' Tm: 59.258
Reverse Primer: 5'-TTTAGGTGGCTGGCTGGTGG-3' Tm: 59.508
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 130, 140
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr11:57225404-57225452

Rank: 18	Homology Score: 2	RVD Match Score: 3.228	Left Half-Site RVD Score: 6.717	Right Half-Site RVD Score: 4.913
	Genomic Region: Intron	Closest Gene: ANKRD28
Match Type: L22R
	           Left Nuclease
	         TTTTCTGTCACCAATCCT
	5'-GACCA TCCTCTGAGACCCATCCT   TTCTCAGAGGCTGGGAAGGGTA   GTTGGGGGATGGAGGGGT GGGGA-3'
	3'-CTGGT AGGAGACTCTGGGTAGGA   AAGAGTCTCCGACCCTTCCCAT   CAACCCCCTACCTCCCCA CCCCT-5'
	                                                       TGACACCCCACCTCCCCT
	                                                            Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 003-015883800
Forward Primer: 5'-GCCAGGCACAAAAAGACAAAGTTTGCATG-3' Tm: 60.178
Reverse Primer: 5'-GGGGTATCCATCACCTCAAGC-3' Tm: 59.378
Expected PCR Product Length: 342
Expected CelI Cleavage Product Sizes: 141, 201
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr3:15883910-15883967

Rank: 19	Homology Score: 2	RVD Match Score: 3.231	Left Half-Site RVD Score: 6.169	Right Half-Site RVD Score: 5.395
	Genomic Region: Intergenic	Closest Gene: MAS1
Match Type: L25R
	           Left Nuclease
	         TTTTCTGTCACCAATCCT
	5'-TGTTT GTTTCTGTCAGAACTCAT GTTGAAATTTGATCTCTAATGTGGC  AGTGTTGGGCGGTGGGGC CTAGT-3'
	3'-ACAAA CAAAGACAGTCTTGAGTA CAACTTTAAACTAGAGATTACACCG  TCACAACCCGCCACCCCG GATCA-5'
	                                                       TGACACCCCACCTCCCCT
	                                                            Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 006-160296301
Forward Primer: 5'-CTGGTGAGCTTTCCCTGTGTAC-3' Tm: 59.258
Reverse Primer: 5'-GAACTCATCTAGTCTCCCCGG-3' Tm: 59.378
Expected PCR Product Length: 275
Expected CelI Cleavage Product Sizes: 142, 133
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr6:160296411-160296471

Rank: 20	Homology Score: 2	RVD Match Score: 3.236	Left Half-Site RVD Score: 5.304	Right Half-Site RVD Score: 6.309
	Genomic Region: Intron	Closest Gene: LGR6
Match Type: R22L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-GATGT CCCCCTCCACTCCAAAAG   GCACAGGCTGTGGGCCAGGTAA   GGGAGTGGGAAGAGAAAA ATAAT-3'
	3'-CTACA GGGGGAGGTGAGGTTTTC   CGTGTCCGACACCCGGTCCATT   CCCTCACCCTTCTCTTTT TATTA-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 001-202164461
Forward Primer: 5'-TGCTCAGTGTTGGGAGTGTCCT-3' Tm: 59.258
Reverse Primer: 5'-TTTGCCAGATGCTGAGCGCC-3' Tm: 59.508
Expected PCR Product Length: 276
Expected CelI Cleavage Product Sizes: 131, 145
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr1:202164561-202164618

Rank: 21	Homology Score: 5	RVD Match Score: 3.245	Left Half-Site RVD Score: 5.045	Right Half-Site RVD Score: 6.688
	Genomic Region: Intergenic	Closest Gene: FBN2
Match Type: R20L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-GACTT CCACCTCCACCTAACAGT    AATGAGATGCCCCTGTCTGC    TGGGATGGTGTCAGAAGA TACCT-3'
	3'-CTGAA GGTGGAGGTGGATTGTCA    TTACTCTACGGGGACAGACG    ACCCTACCACAGTCTTCT ATGGA-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 005-127879056
Forward Primer: 5'-CTCCTGGATAGTGTCAGAGAAAGC-3' Tm: 59.038
Reverse Primer: 5'-CTGCTCCCAATGTAGTTTCCCATG-3' Tm: 59.038
Expected PCR Product Length: 267
Expected CelI Cleavage Product Sizes: 136, 131
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr5:127879162-127879217

Rank: 22	Homology Score: 5	RVD Match Score: 3.245	Left Half-Site RVD Score: 6.031	Right Half-Site RVD Score: 5.614
	Genomic Region: Intergenic	Closest Gene: RPRM
Match Type: L14R
	           Left Nuclease
	         TTTTCTGTCACCAATCCT
	5'-ACTGT TTTTCTGACAGAAACCCT       TTGCAGAGTTTTTG       GCTGGGGGGTGGGGAGGT GGTTG-3'
	3'-TGACA AAAAGACTGTCTTTGGGA       AACGTCTCAAAAAC       CGACCCCCCACCCCTCCA CCAAC-5'
	                                                       TGACACCCCACCTCCCCT
	                                                            Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 002-154289086
Forward Primer: 5'-GGTTTCAAGTTTCACCTATTGCTTGGCAG-3' Tm: 60.178
Reverse Primer: 5'-CGTGTTATGTGTGTTTAGCCCCC-3' Tm: 59.148
Expected PCR Product Length: 411
Expected CelI Cleavage Product Sizes: 134, 277
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr2:154289193-154289242

Rank: 23	Homology Score: 2	RVD Match Score: 3.249	Left Half-Site RVD Score: 6.443	Right Half-Site RVD Score: 5.278
	Genomic Region: Intron	Closest Gene: GRIK3
Match Type: L22R
	           Left Nuclease
	         TTTTCTGTCACCAATCCT
	5'-CTGGC ATGGCTGTCATCAACCCT   GCCAGCAGGACAGGAAGGGGCA   GGTGTGGGGCGGTGGGGT ACAGG-3'
	3'-GACCG TACCGACAGTAGTTGGGA   CGGTCGTCCTGTCCTTCCCCGT   CCACACCCCGCCACCCCA TGTCC-5'
	                                                       TGACACCCCACCTCCCCT
	                                                            Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 001-037420737
Forward Primer: 5'-CACTGATTTGCTGAGGCTCAGATG-3' Tm: 59.038
Reverse Primer: 5'-GCCTCTGTGTATCCATCCACG-3' Tm: 59.378
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 140, 130
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr1:37420846-37420903

Rank: 24	Homology Score: 5	RVD Match Score: 3.25	Left Half-Site RVD Score: 6.391	Right Half-Site RVD Score: 5.326
	Genomic Region: Intergenic	Closest Gene: MRM1
Match Type: L25R
	           Left Nuclease
	         TTTTCTGTCACCAATCCT
	5'-TTCTT TTATCTGCCCCCAACACT CCAGGCATTATGTGCATTCATAAAA  AAGGGGGGGTGGAGTGGA GAGAA-3'
	3'-AAGAA AATAGACGGGGGTTGTGA GGTCCGTAATACACGTAAGTATTTT  TTCCCCCCCACCTCACCT CTCTT-5'
	                                                       TGACACCCCACCTCCCCT
	                                                            Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 017-035084330
Forward Primer: 5'-GAGCCTCTGCTGGTTGGATG-3' Tm: 59.508
Reverse Primer: 5'-ATGTTGGGTGCTGGGGAAGG-3' Tm: 59.508
Expected PCR Product Length: 287
Expected CelI Cleavage Product Sizes: 131, 156
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr17:35084429-35084489

Rank: 25	Homology Score: 5	RVD Match Score: 3.264	Left Half-Site RVD Score: 5.57	Right Half-Site RVD Score: 6.222
	Genomic Region: Intergenic	Closest Gene: ABRA
Match Type: R12L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-CTGTT TCCCCACCCTCCCACATT        ATGTACCCTGGG        AGGAAGGGTGCCAGAGAG AGGCT-3'
	3'-GACAA AGGGGTGGGAGGGTGTAA        TACATGGGACCC        TCCTTCCCACGGTCTCTC TCCGA-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 008-107822970
Forward Primer: 5'-GGCTAAGTGTGAGAATCGAGTGAG-3' Tm: 59.038
Reverse Primer: 5'-GTTAGTGAGACAGCATGTCCCTTC-3' Tm: 59.038
Expected PCR Product Length: 280
Expected CelI Cleavage Product Sizes: 150, 130
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr8:107823094-107823141

Rank: 26	Homology Score: 2	RVD Match Score: 3.268	Left Half-Site RVD Score: 5.466	Right Half-Site RVD Score: 6.369
	Genomic Region: Intron	Closest Gene: SMOC2
Match Type: R25L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-CCTCC CCCCCTCCCCCACAAAGA TTTGAAGCCTAACATTGCTGGGGGC  AGGATTAGAGGTTGAAAA CAAAG-3'
	3'-GGAGG GGGGGAGGGGGTGTTTCT AAACTTCGGATTGTAACGACCCCCG  TCCTAATCTCCAACTTTT GTTTC-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 006-168932997
Forward Primer: 5'-TCCTTCGTCCTGGGATTGGG-3' Tm: 59.508
Reverse Primer: 5'-GGGTGCACAGTTACAGAGGAG-3' Tm: 59.378
Expected PCR Product Length: 280
Expected CelI Cleavage Product Sizes: 149, 131
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr6:168933114-168933174

Rank: 27	Homology Score: 2	RVD Match Score: 3.268	Left Half-Site RVD Score: 5.827	Right Half-Site RVD Score: 5.97
	Genomic Region: Intergenic	Closest Gene: MIR5580
Match Type: R22L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-AAGCA GCCATTCCACCCCAAAGC   CCTTGTGTTCCTCAATATCTTC   AGAATTGTTGTCAGAAGC CACTC-3'
	3'-TTCGT CGGTAAGGTGGGGTTTCG   GGAACACAAGGAGTTATAGAAG   TCTTAACAACAGTCTTCG GTGAG-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 014-054175647
Forward Primer: 5'-GCCAGAACAGCCTTGTGGATAC-3' Tm: 59.258
Reverse Primer: 5'-GACAGGATGGGACGCTGATAG-3' Tm: 59.378
Expected PCR Product Length: 310
Expected CelI Cleavage Product Sizes: 179, 131
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr14:54175795-54175852

Rank: 28	Homology Score: 5	RVD Match Score: 3.274	Left Half-Site RVD Score: 7.511	Right Half-Site RVD Score: 4.588
	Genomic Region: Intron	Closest Gene: LRRC4C
Match Type: L14R
	           Left Nuclease
	         TTTTCTGTCACCAATCCT
	5'-TAAAA TTGTCTGTCACACATTGT       TCTGTTAATTGCCA       ACTGGGGGGGGTAGGGGG AGGAA-3'
	3'-ATTTT AACAGACAGTGTGTAACA       AGACAATTAACGGT       TGACCCCCCCCATCCCCC TCCTT-5'
	                                                       TGACACCCCACCTCCCCT
	                                                            Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 011-040808649
Forward Primer: 5'-CCCATGTCTCAGTCTCTGTTCTAC-3' Tm: 59.038
Reverse Primer: 5'-CTCACTTGTCAGCAAGCACAAAGG-3' Tm: 59.038
Expected PCR Product Length: 309
Expected CelI Cleavage Product Sizes: 155, 154
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr11:40808777-40808826

Rank: 29	Homology Score: 2	RVD Match Score: 3.283	Left Half-Site RVD Score: 5.792	Right Half-Site RVD Score: 6.119
	Genomic Region: Intergenic	Closest Gene: WDR59
Match Type: R16L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-CCAAT GGCCCTCCACCACCCAGC      CATTATCTAATATTCT      GGGATTGTTTAGGGAAAA TGTTT-3'
	3'-GGTTA CCGGGAGGTGGTGGGTCG      GTAATAGATTATAAGA      CCCTAACAAATCCCTTTT ACAAA-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 016-074868261
Forward Primer: 5'-TCAAGAATCAGGATGTCAGGGGAG-3' Tm: 59.038
Reverse Primer: 5'-CTACTGGAAAGTTCTACTGGACGG-3' Tm: 59.038
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 135, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr16:74868368-74868419

Rank: 30	Homology Score: 2	RVD Match Score: 3.284	Left Half-Site RVD Score: 5.726	Right Half-Site RVD Score: 6.172
	Genomic Region: Intron	Closest Gene: CBFB
Match Type: L18R
	           Left Nuclease
	         TTTTCTGTCACCAATCCT
	5'-TTTCA GTCTCTGTCACAACTACT     CATCTCTGTAACTCTGCT     GTTGTGGTGTGAAAGGGA CCCTA-3'
	3'-AAAGT CAGAGACAGTGTTGATGA     GTAGAGACATTGAGACGA     CAACACCACACTTTCCCT GGGAT-5'
	                                                       TGACACCCCACCTCCCCT
	                                                            Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 016-067073560
Forward Primer: 5'-GAGTGATAGAACTATGCAGGCTATCAGG-3' Tm: 60.298
Reverse Primer: 5'-GCACGGAACAGTACAGTACGAG-3' Tm: 59.258
Expected PCR Product Length: 393
Expected CelI Cleavage Product Sizes: 144, 249
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr16:67073675-67073728

Rank: 31	Homology Score: 2	RVD Match Score: 3.288	Left Half-Site RVD Score: 4.892	Right Half-Site RVD Score: 7.228
	Genomic Region: Intron	Closest Gene: PKHD1L1
Match Type: R15L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-CCCCC CTCCCCCCACCCCACAAC      AGGCCCCAGGGACAC       AGGAAGGGGAACATAAAA CATAG-3'
	3'-GGGGG GAGGGGGGTGGGGTGTTG      TCCGGGGTCCCTGTG       TCCTTCCCCTTGTATTTT GTATC-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 008-110449611
Forward Primer: 5'-GTGCACAACGTGCAGGTTAGTTAC-3' Tm: 59.038
Reverse Primer: 5'-CCTGCAGATTGCAAATACATAATACCCCC-3' Tm: 60.178
Expected PCR Product Length: 293
Expected CelI Cleavage Product Sizes: 138, 155
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr8:110449722-110449772

Rank: 32	Homology Score: 5	RVD Match Score: 3.29	Left Half-Site RVD Score: 6.091	Right Half-Site RVD Score: 5.853
	Genomic Region: Exon	Closest Gene: CATSPERG
Match Type: R24L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-TCAAT CCCCTCCAACCCCATAGT  TTTCTACCCCTTCTTCTTGATTCA  AGATTTGGTGACAGGAGA CTCCG-3'
	3'-AGTTA GGGGAGGTTGGGGTATCA  AAAGATGGGGAAGAAGAACTAAGT  TCTAAACCACTGTCCTCT GAGGC-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 019-038858026
Forward Primer: 5'-TTCCTCTCCCCGCAGACTAC-3' Tm: 59.508
Reverse Primer: 5'-AGTGCACACAGACTGGCCTC-3' Tm: 59.508
Expected PCR Product Length: 281
Expected CelI Cleavage Product Sizes: 138, 143
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr19:38858132-38858191

Rank: 33	Homology Score: 10	RVD Match Score: 3.291	Left Half-Site RVD Score: 7.204	Right Half-Site RVD Score: 4.928
	Genomic Region: Intron	Closest Gene: ERCC5
Match Type: L20R
	           Left Nuclease
	         TTTTCTGTCACCAATCCT
	5'-CTTTA TTTTCTTTCTGCAACCAT    GAAGTCTCTGGAAGTGGTGG    ACTGTAGGGTGGTGTGGA GTAGC-3'
	3'-GAAAT AAAAGAAAGACGTTGGTA    CTTCAGAGACCTTCACCACC    TGACATCCCACCACACCT CATCG-5'
	                                                       TGACACCCCACCTCCCCT
	                                                            Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 013-103511881
Forward Primer: 5'-TCTCTGGCTGGCAGTTGAGG-3' Tm: 59.508
Reverse Primer: 5'-CAGGTCACTGAAAACTGCAAACCG-3' Tm: 59.038
Expected PCR Product Length: 346
Expected CelI Cleavage Product Sizes: 131, 215
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr13:103511982-103512037

Rank: 34	Homology Score: 5	RVD Match Score: 3.295	Left Half-Site RVD Score: 6.076	Right Half-Site RVD Score: 5.904
	Genomic Region: Intron	Closest Gene: HEG1
Match Type: R18L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-CTCTA TCCCTTCCACCTCACCAA     ATAGTCCTCGCTAGCAGA     AGGGTTGGGTACAGAACA ACAGT-3'
	3'-GAGAT AGGGAAGGTGGAGTGGTT     TATCAGGAGCGATCGTCT     TCCCAACCCATGTCTTGT TGTCA-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 003-124702567
Forward Primer: 5'-TGCAAGTCTACAACCAGGGTGTG-3' Tm: 59.148
Reverse Primer: 5'-CAGAAGGCAGCTGGAAGTCTC-3' Tm: 59.378
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 134, 136
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr3:124702672-124702725

Rank: 35	Homology Score: 5	RVD Match Score: 3.296	Left Half-Site RVD Score: 6.1	Right Half-Site RVD Score: 5.891
	Genomic Region: Intron	Closest Gene: TMEM9
Match Type: R20L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-GCAGC CACCCTCTTCCCCACATT    CCTTCTCCAACTTCTTAGGT    AGGATTGGGGAAACATAA AGGGC-3'
	3'-CGTCG GTGGGAGAAGGGGTGTAA    GGAAGAGGTTGAAGAATCCA    TCCTAACCCCTTTGTATT TCCCG-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 001-201121271
Forward Primer: 5'-GAGGGATGAGGGGAAATGGG-3' Tm: 59.508
Reverse Primer: 5'-GGAGCTGTTTGGGTAGATCTATGG-3' Tm: 59.038
Expected PCR Product Length: 373
Expected CelI Cleavage Product Sizes: 131, 242
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr1:201121372-201121427

Rank: 36	Homology Score: 2	RVD Match Score: 3.297	Left Half-Site RVD Score: 6.148	Right Half-Site RVD Score: 5.865
	Genomic Region: Intergenic	Closest Gene: PTGER4
Match Type: L20R
	           Left Nuclease
	         TTTTCTGTCACCAATCCT
	5'-TTTGT CATGCTGTCACCAATCTC    TCGGCTTGTATGTAAGGTCT    CATGGGGAGTGGAGGGGT AGTTC-3'
	3'-AAACA GTACGACAGTGGTTAGAG    AGCCGAACATACATTCCAGA    GTACCCCTCACCTCCCCA TCAAG-5'
	                                                       TGACACCCCACCTCCCCT
	                                                            Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 005-040515613
Forward Primer: 5'-GTTGAAAAGTGGGGAAAGGGGG-3' Tm: 59.258
Reverse Primer: 5'-CTTTAGGACCACTCCTGAATGGG-3' Tm: 59.148
Expected PCR Product Length: 290
Expected CelI Cleavage Product Sizes: 137, 153
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr5:40515720-40515775

Rank: 37	Homology Score: 2	RVD Match Score: 3.303	Left Half-Site RVD Score: 5.935	Right Half-Site RVD Score: 6.107
	Genomic Region: Intron	Closest Gene: KIAA0556
Match Type: L21R
	           Left Nuclease
	         TTTTCTGTCACCAATCCT
	5'-GGACC CATTCAGTGACCACTCCT   ATAGTTCCCTAGTTCACTTGT    CTTGTGGGGGTGAGAGGA GGGAC-3'
	3'-CCTGG GTAAGTCACTGGTGAGGA   TATCAAGGGATCAAGTGAACA    GAACACCCCCACTCTCCT CCCTG-5'
	                                                       TGACACCCCACCTCCCCT
	                                                            Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 016-027592598
Forward Primer: 5'-CCCTGTGCACATAGGAATAGCC-3' Tm: 59.258
Reverse Primer: 5'-GGGTGATGGGTGCATCAATATCTC-3' Tm: 59.038
Expected PCR Product Length: 416
Expected CelI Cleavage Product Sizes: 241, 175
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr16:27592809-27592865

Rank: 38	Homology Score: 2	RVD Match Score: 3.304	Left Half-Site RVD Score: 6.558	Right Half-Site RVD Score: 5.485
	Genomic Region: Intergenic	Closest Gene: LOC100144595
Match Type: R26L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-TTCTT TCCACTCCACCTCTCACC TATGCAGTTTCAACTTCCACATGGAC AGGATTGGTGAGTAGAAT AGGAC-3'
	3'-AAGAA AGGTGAGGTGGAGAGTGG ATACGTCAAAGTTGAAGGTGTACCTG TCCTAACCACTCATCTTA TCCTG-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 002-155321775
Forward Primer: 5'-TACCCCCCACCCCCTTGATA-3' Tm: 59.508
Reverse Primer: 5'-GAAGGTGGTGTCACTTGTCTTCC-3' Tm: 59.148
Expected PCR Product Length: 338
Expected CelI Cleavage Product Sizes: 133, 205
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr2:155321875-155321936

Rank: 39	Homology Score: 2	RVD Match Score: 3.307	Left Half-Site RVD Score: 6.769	Right Half-Site RVD Score: 5.388
	Genomic Region: Intron	Closest Gene: SLC24A3
Match Type: L18R
	           Left Nuclease
	         TTTTCTGTCACCAATCCT
	5'-CCATT CTTGGTGTCAGAAATCCT     TGGGCTCTCTAAGCCCCA     GTTGTGGGGAGGAGGGCG GTAGG-3'
	3'-GGTAA GAACCACAGTCTTTAGGA     ACCCGAGAGATTCGGGGT     CAACACCCCTCCTCCCGC CATCC-5'
	                                                       TGACACCCCACCTCCCCT
	                                                            Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 020-019256133
Forward Primer: 5'-GGAAGGGTTTGCAAGCCCTG-3' Tm: 59.508
Reverse Primer: 5'-AAGCAAGCCGCATGCACTAAGG-3' Tm: 59.258
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 135, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr20:19256239-19256292

Rank: 40	Homology Score: 2	RVD Match Score: 3.308	Left Half-Site RVD Score: 6.288	Right Half-Site RVD Score: 5.812
	Genomic Region: Intergenic	Closest Gene: SUCLG1
Match Type: L13R
	           Left Nuclease
	         TTTTCTGTCACCAATCCT
	5'-AATGA GCTTCTGCCTCTAATCCT       GATGATGATAATG        ATGATGGTGTGGTGGGGA TGGTA-3'
	3'-TTACT CGAAGACGGAGATTAGGA       CTACTACTATTAC        TACTACCACACCACCCCT ACCAT-5'
	                                                       TGACACCCCACCTCCCCT
	                                                            Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 002-084649832
Forward Primer: 5'-AAAGAATGTGGGCTCTGGATGGG-3' Tm: 59.148
Reverse Primer: 5'-GAGGCTGTAACAGTGTCAACGTG-3' Tm: 59.148
Expected PCR Product Length: 352
Expected CelI Cleavage Product Sizes: 130, 222
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr2:84649936-84649984

Rank: 41	Homology Score: 5	RVD Match Score: 3.309	Left Half-Site RVD Score: 6.739	Right Half-Site RVD Score: 5.424
	Genomic Region: Intergenic	Closest Gene: ACBD3
Match Type: L13R
	           Left Nuclease
	         TTTTCTGTCACCAATCCT
	5'-TTTTA TTTTCTGAGATGAATCCT       CGCTCTGTTGCCC        AGGGTGGGGTGCAGTGGC GCAAT-3'
	3'-AAAAT AAAAGACTCTACTTAGGA       GCGAGACAACGGG        TCCCACCCCACGTCACCG CGTTA-5'
	                                                       TGACACCCCACCTCCCCT
	                                                            Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 001-226315854
Forward Primer: 5'-AAGCGCCCTCAGCAACCTCA-3' Tm: 59.508
Reverse Primer: 5'-ATTTAGCCAACCGTGGTGGCAG-3' Tm: 59.258
Expected PCR Product Length: 271
Expected CelI Cleavage Product Sizes: 140, 131
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr1:226315968-226316016

Rank: 42	Homology Score: 5	RVD Match Score: 3.316	Left Half-Site RVD Score: 6.275	Right Half-Site RVD Score: 5.88
	Genomic Region: Intergenic	Closest Gene: LOC729013
Match Type: L25R
	           Left Nuclease
	         TTTTCTGTCACCAATCCT
	5'-AGGTC TCCTCTGACACCACTCCA GTGAGTAGTGGAAAGATGCCTTATT  ACTGCTGGGTGGGGGTGA AGGTC-3'
	3'-TCCAG AGGAGACTGTGGTGAGGT CACTCATCACCTTTCTACGGAATAA  TGACGACCCACCCCCACT TCCAG-5'
	                                                       TGACACCCCACCTCCCCT
	                                                            Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 011-011075984
Forward Primer: 5'-GCCTCATTACTGCTAGGTGGAAG-3' Tm: 59.148
Reverse Primer: 5'-CACCCTAACATCCAGGCATGATGA-3' Tm: 59.038
Expected PCR Product Length: 316
Expected CelI Cleavage Product Sizes: 152, 164
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr11:11076104-11076164

Rank: 43	Homology Score: 2	RVD Match Score: 3.317	Left Half-Site RVD Score: 6.352	Right Half-Site RVD Score: 5.788
	Genomic Region: Intron	Closest Gene: CPN1
Match Type: R11L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-CCCAC TCCCTCCCACCCCACCTA        CCATGAAGAAT         AGGATTGGGGGCAGGACT GAGCT-3'
	3'-GGGTG AGGGAGGGTGGGGTGGAT        GGTACTTCTTA         TCCTAACCCCCGTCCTGA CTCGA-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 010-101836357
Forward Primer: 5'-CAGCCGCTAATGATCCCAGTTG-3' Tm: 59.258
Reverse Primer: 5'-GACATAGCAGGTGCCTTCCTATTC-3' Tm: 59.038
Expected PCR Product Length: 308
Expected CelI Cleavage Product Sizes: 177, 131
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr10:101836509-101836555

Rank: 44	Homology Score: 5	RVD Match Score: 3.319	Left Half-Site RVD Score: 5.305	Right Half-Site RVD Score: 6.964
	Genomic Region: Intron	Closest Gene: LOC730102
Match Type: R25L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-AACCA TCCCCTCCAACCCCCATC AGTCCATGGAAAAACTGTCTTCCAC  AGAACTGGTGCCAAAAAG GTTGC-3'
	3'-TTGGT AGGGGAGGTTGGGGGTAG TCAGGTACCTTTTTGACAGAAGGTG  TCTTGACCACGGTTTTTC CAACG-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 001-178005473
Forward Primer: 5'-CTCACAGAAGTGCGAACCGTATTG-3' Tm: 59.038
Reverse Primer: 5'-CACCAATATGGCCACCTCTGTAC-3' Tm: 59.148
Expected PCR Product Length: 315
Expected CelI Cleavage Product Sizes: 148, 167
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr1:178005589-178005649

Rank: 45	Homology Score: 2	RVD Match Score: 3.32	Left Half-Site RVD Score: 6.486	Right Half-Site RVD Score: 5.719
	Genomic Region: Intergenic	Closest Gene: AXIN2
Match Type: L20R
	           Left Nuclease
	         TTTTCTGTCACCAATCCT
	5'-AAAGT TTTTCCATCAACATTTCT    AAATGGAAGAGAGAAGGCAA    AAAGTGGGGTGGGGGTGG GAGGG-3'
	3'-TTTCA AAAAGGTAGTTGTAAAGA    TTTACCTTCTCTCTTCCGTT    TTTCACCCCACCCCCACC CTCCC-5'
	                                                       TGACACCCCACCTCCCCT
	                                                            Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 017-063492561
Forward Primer: 5'-CTCTGTTCCCCACTGATCACC-3' Tm: 59.378
Reverse Primer: 5'-AGCATGGAAGGTCCCCCACA-3' Tm: 59.508
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 135, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr17:63492666-63492721

Rank: 46	Homology Score: 5	RVD Match Score: 3.32	Left Half-Site RVD Score: 5.961	Right Half-Site RVD Score: 6.222
	Genomic Region: Intergenic	Closest Gene: ZNF827
Match Type: R20L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-AGATT TCCTCTCCACACCACATG    GCCACTGCTGGGGCTGGGGG    AGGAGAGGTGTCAGCAAT TCAAG-3'
	3'-TCTAA AGGAGAGGTGTGGTGTAC    CGGTGACGACCCCGACCCCC    TCCTCTCCACAGTCGTTA AGTTC-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 004-146944903
Forward Primer: 5'-CAGAGGAGTTCAGCTCAGTTTTGC-3' Tm: 59.038
Reverse Primer: 5'-CCCTCCCCCAAGGACAACAA-3' Tm: 59.508
Expected PCR Product Length: 307
Expected CelI Cleavage Product Sizes: 135, 172
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr4:146945008-146945063

Rank: 47	Homology Score: 5	RVD Match Score: 3.323	Left Half-Site RVD Score: 6.587	Right Half-Site RVD Score: 5.595
	Genomic Region: Intron	Closest Gene: PVRL1
Match Type: R17L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-TAAAG TCCTCTGCCACCCACACT     CTTCATGAGATCCCAAA      AGGATTGATCATGGAAAA TGCAA-3'
	3'-ATTTC AGGAGACGGTGGGTGTGA     GAAGTACTCTAGGGTTT      TCCTAACTAGTACCTTTT ACGTT-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 011-119574363
Forward Primer: 5'-CAACGAGGCTGACGCTCTTTC-3' Tm: 59.378
Reverse Primer: 5'-CTGAGCACCTGTGTGCCAGT-3' Tm: 59.508
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 135, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr11:119574470-119574522

Rank: 48	Homology Score: 5	RVD Match Score: 3.323	Left Half-Site RVD Score: 5.305	Right Half-Site RVD Score: 6.894
	Genomic Region: Intergenic	Closest Gene: FAXC
Match Type: L26R
	           Left Nuclease
	         TTTTCTGTCACCAATCCT
	5'-TGTCC ATTTCTGTAACAAATCTT TATTGAGCATTTATTACATGTACCAA GCTGTGAGGGGAAGTGGA TTGTG-3'
	3'-ACAGG TAAAGACATTGTTTAGAA ATAACTCGTAAATAATGTACATGGTT CGACACTCCCCTTCACCT AACAC-5'
	                                                       TGACACCCCACCTCCCCT
	                                                            Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 006-099703892
Forward Primer: 5'-GGACTGCATAAACCACTTGAGCC-3' Tm: 59.148
Reverse Primer: 5'-GCAGGACTCTCTAGCTGGCTAT-3' Tm: 59.258
Expected PCR Product Length: 330
Expected CelI Cleavage Product Sizes: 200, 130
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr6:99704059-99704120

Rank: 49	Homology Score: 2	RVD Match Score: 3.328	Left Half-Site RVD Score: 6.112	Right Half-Site RVD Score: 6.119
	Genomic Region: Intron	Closest Gene: AUTS2
Match Type: R12L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-ACCCA CCCCTTCCCTCACACAGT        CTCAGGCAATTA        TTGATTTGTGAGGGAAAA GGGGT-3'
	3'-TGGGT GGGGAAGGGAGTGTGTCA        GAGTCCGTTAAT        AACTAAACACTCCCTTTT CCCCA-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 007-069107597
Forward Primer: 5'-GATGTCAGTGTCCTCAGCTGTTAG-3' Tm: 59.038
Reverse Primer: 5'-CCTGACTCACAGAAACTGTGGG-3' Tm: 59.258
Expected PCR Product Length: 319
Expected CelI Cleavage Product Sizes: 156, 163
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr7:69107727-69107774

Rank: 50	Homology Score: 2	RVD Match Score: 3.331	Left Half-Site RVD Score: 6.028	Right Half-Site RVD Score: 6.229
	Genomic Region: Intergenic	Closest Gene: ADTRP
Match Type: R14L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-TAGCT TCCCTCCCACCCCCCACC       CTGGAACAACAGAT       TGGGTTAGTGCTAGAAAA TGTCT-3'
	3'-ATCGA AGGGAGGGTGGGGGGTGG       GACCTTGTTGTCTA       ACCCAATCACGATCTTTT ACAGA-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Warning! Primer pair was generated using suboptimal parameters. 
Primer Index: 006-011853744
Forward Primer: 5'-GTCTACGGATCTAACACTCCAAAAATCTTAGC-3' Tm: 59.848
Reverse Primer: 5'-CTAGGGGGCTGAAATGGAACAAC-3' Tm: 59.148
Expected PCR Product Length: 325
Expected CelI Cleavage Product Sizes: 172, 153
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr6:11853889-11853938

Rank: 51	Homology Score: 2	RVD Match Score: 3.332	Left Half-Site RVD Score: 6.732	Right Half-Site RVD Score: 5.591
	Genomic Region: Promoter	Closest Gene: RASSF5
Match Type: L25R
	           Left Nuclease
	         TTTTCTGTCACCAATCCT
	5'-TTTTT TCTTCTCTAACCATTCCG ATTGACCCGGCTCCCCCACCCAGCG  AATGGGGGAGGGAGGGGG TGGGG-3'
	3'-AAAAA AGAAGAGATTGGTAAGGC TAACTGGGCCGAGGGGGTGGGTCGC  TTACCCCCTCCCTCCCCC ACCCC-5'
	                                                       TGACACCCCACCTCCCCT
	                                                            Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 001-206729981
Forward Primer: 5'-CTCTCTAGGTTGCCATAACTCTGC-3' Tm: 59.038
Reverse Primer: 5'-TCTCATCAGCCCCGGTGACT-3' Tm: 59.508
Expected PCR Product Length: 287
Expected CelI Cleavage Product Sizes: 155, 132
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr1:206730104-206730164

Rank: 52	Homology Score: 5	RVD Match Score: 3.335	Left Half-Site RVD Score: 4.892	Right Half-Site RVD Score: 7.621
	Genomic Region: Intron	Closest Gene: ARHGAP10
Match Type: R17L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-TCCTG TTCCCACCACCCCACAAC     CTTCTTACATCTGAACA      ATGACAGATGACAGTAAA TCCAG-3'
	3'-AGGAC AAGGGTGGTGGGGTGTTG     GAAGAATGTAGACTTGT      TACTGTCTACTGTCATTT AGGTC-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 004-148942680
Forward Primer: 5'-GTAGGTAAGCCAGGGAGCTGT-3' Tm: 59.378
Reverse Primer: 5'-CCTGCCAACACCATGTTTCCAC-3' Tm: 59.258
Expected PCR Product Length: 299
Expected CelI Cleavage Product Sizes: 158, 141
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr4:148942810-148942862

Rank: 53	Homology Score: 2	RVD Match Score: 3.345	Left Half-Site RVD Score: 6.409	Right Half-Site RVD Score: 5.94
	Genomic Region: Intergenic	Closest Gene: BUD13
Match Type: R23L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-CACCA TCTCCCTCCCCCCACACT  CCCATACCCCAATCCTCCTAGGC   AGAATGGGAGAGAGAAAG AAAGA-3'
	3'-GTGGT AGAGGGAGGGGGGTGTGA  GGGTATGGGGTTAGGAGGATCCG   TCTTACCCTCTCTCTTTC TTTCT-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 011-116565112
Forward Primer: 5'-CATCTATGCCCCACCAAGAGG-3' Tm: 59.378
Reverse Primer: 5'-ACATCTGTGGCAGGTGGAGC-3' Tm: 59.508
Expected PCR Product Length: 279
Expected CelI Cleavage Product Sizes: 137, 142
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr11:116565218-116565276

Rank: 54	Homology Score: 2	RVD Match Score: 3.346	Left Half-Site RVD Score: 5.788	Right Half-Site RVD Score: 6.567
	Genomic Region: Intron	Closest Gene: ABCB7
Match Type: L21R
	           Left Nuclease
	         TTTTCTGTCACCAATCCT
	5'-CCTAA ATTGCTGACACCACCCCT   CCCCGACACCGCAGAAGCAGT    GGTGTGGTGTGGAGAGCA TCTCT-3'
	3'-GGATT TAACGACTGTGGTGGGGA   GGGGCTGTGGCGTCTTCGTCA    CCACACCACACCTCTCGT AGAGA-5'
	                                                       TGACACCCCACCTCCCCT
	                                                            Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 00X-074326678
Forward Primer: 5'-CCCTTCCCTTCAAGGACACC-3' Tm: 59.508
Reverse Primer: 5'-GCTGAGTTTCGTCCTGTTTCTGG-3' Tm: 59.148
Expected PCR Product Length: 306
Expected CelI Cleavage Product Sizes: 175, 131
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chrX:74326823-74326879

Rank: 55	Homology Score: 2	RVD Match Score: 3.347	Left Half-Site RVD Score: 6.372	Right Half-Site RVD Score: 5.987
	Genomic Region: Intergenic	Closest Gene: TMPRSS15
Match Type: R11L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-AAATT TTCCCTCCACCCTCCAAG        CATTTGATAAT         AGGGTTGCTGAAATAAAC TGAGT-3'
	3'-TTTAA AAGGGAGGTGGGAGGTTC        GTAAACTATTA         TCCCAACGACTTTATTTG ACTCA-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 021-020928381
Forward Primer: 5'-GGTGAACTGGGGTCCCAAGTTT-3' Tm: 59.258
Reverse Primer: 5'-CTGAGGGCCTACTCATCTTTCTTC-3' Tm: 59.038
Expected PCR Product Length: 338
Expected CelI Cleavage Product Sizes: 132, 206
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr21:20928488-20928534

Rank: 56	Homology Score: 2	RVD Match Score: 3.347	Left Half-Site RVD Score: 6.576	Right Half-Site RVD Score: 5.827
	Genomic Region: Intron	Closest Gene: LRP5
Match Type: L15R
	           Left Nuclease
	         TTTTCTGTCACCAATCCT
	5'-AGCGC TTTTCTATGAAGCATCCT      GTTTAAGCCTCGCAC       TCTGTGATGTGGAGGTGC AGTAA-3'
	3'-TCGCG AAAAGATACTTCGTAGGA      CAAATTCGGAGCGTG       AGACACTACACCTCCACG TCATT-5'
	                                                       TGACACCCCACCTCCCCT
	                                                            Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 011-068107851
Forward Primer: 5'-TCTGAGTGGATGGTGTGTGCTTTG-3' Tm: 59.038
Reverse Primer: 5'-AGAGCAGACGGCAGACTCCA-3' Tm: 59.508
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 136, 134
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr11:68107960-68108010

Rank: 57	Homology Score: 2	RVD Match Score: 3.347	Left Half-Site RVD Score: 6.173	Right Half-Site RVD Score: 6.201
	Genomic Region: Intron	Closest Gene: ZNF676
Match Type: R24L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-CCCAA ACTCCTCCCAGCCACAGT  CTGTGAGAGGTCTTGGTCCTCCAG  AGGCTTGGAGACTGATAC CCATT-3'
	3'-GGGTT TGAGGAGGGTCGGTGTCA  GACACTCTCCAGAACCAGGAGGTC  TCCGAACCTCTGACTATG GGTAA-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 019-022374062
Forward Primer: 5'-AGGTGGCAAACTAGAGGACCC-3' Tm: 59.378
Reverse Primer: 5'-ACTGGCCATGAACTGTGGCTTAG-3' Tm: 59.148
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 135, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr19:22374165-22374224

Rank: 58	Homology Score: 2	RVD Match Score: 3.348	Left Half-Site RVD Score: 6.255	Right Half-Site RVD Score: 6.117
	Genomic Region: Intron	Closest Gene: CPEB2
Match Type: R22L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-TTTCC TTCCCTGCCCCCAACAAT   TTGTAAAAAGGCTTACTGTGTG   AGTATTTTTGCCAGAAAT TGCTT-3'
	3'-AAAGG AAGGGACGGGGGTTGTTA   AACATTTTTCCGAATGACACAC   TCATAAAAACGGTCTTTA ACGAA-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Warning! Primer pair was generated using suboptimal parameters. 
Primer Index: 004-015022872
Forward Primer: 5'-CCTATTTTCTGGTGACTATCTTGATTCTTGGC-3' Tm: 59.848
Reverse Primer: 5'-CAGATCTTCCCCCAAACCGTTAC-3' Tm: 59.148
Expected PCR Product Length: 411
Expected CelI Cleavage Product Sizes: 264, 147
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr4:15023105-15023162

Rank: 59	Homology Score: 2	RVD Match Score: 3.349	Left Half-Site RVD Score: 6.288	Right Half-Site RVD Score: 6.1
	Genomic Region: Exon	Closest Gene: CBLN2
Match Type: L13R
	           Left Nuclease
	         TTTTCTGTCACCAATCCT
	5'-CTATA TTTTCTCTCCTCAACCCC       AGCAAGAAAAATA        AAAGTGGGGTTGAGGAGG GAGAG-3'
	3'-GATAT AAAAGAGAGGAGTTGGGG       TCGTTCTTTTTAT        TTTCACCCCAACTCCTCC CTCTC-5'
	                                                       TGACACCCCACCTCCCCT
	                                                            Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 018-070204440
Forward Primer: 5'-CCACTCAGGAACACCAGGTTCT-3' Tm: 59.258
Reverse Primer: 5'-CATTGGGAACCTGTCAAGAAGTGC-3' Tm: 59.038
Expected PCR Product Length: 419
Expected CelI Cleavage Product Sizes: 267, 152
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr18:70204681-70204729

Rank: 60	Homology Score: 5	RVD Match Score: 3.351	Left Half-Site RVD Score: 6.125	Right Half-Site RVD Score: 6.27
	Genomic Region: Intergenic	Closest Gene: TMTC1
Match Type: L12R
	           Left Nuclease
	         TTTTCTGTCACCAATCCT
	5'-GATTA TTTTCTAACAACCTTCCT        ACTGAGGAACAT        ACTCTGGGATGGATTGGA AGTGC-3'
	3'-CTAAT AAAAGATTGTTGGAAGGA        TGACTCCTTGTA        TGAGACCCTACCTAACCT TCACG-5'
	                                                       TGACACCCCACCTCCCCT
	                                                            Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 012-030180063
Forward Primer: 5'-CTATAAGGCTGCTGGGCCTTTC-3' Tm: 59.258
Reverse Primer: 5'-GAGGCAAACCTACTTTATGGAGGG-3' Tm: 59.038
Expected PCR Product Length: 333
Expected CelI Cleavage Product Sizes: 131, 202
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr12:30180168-30180215

Rank: 61	Homology Score: 5	RVD Match Score: 3.352	Left Half-Site RVD Score: 5.506	Right Half-Site RVD Score: 6.897
	Genomic Region: Intron	Closest Gene: BOP1
Match Type: L22R
	           Left Nuclease
	         TTTTCTGTCACCAATCCT
	5'-CCAAA TTTCCTCTCCCTAATCCT   GCAGGAGCCCACAGCTGGCCAG   AGGGAGGGGAGGAGAGGA CAAGC-3'
	3'-GGTTT AAAGGAGAGGGATTAGGA   CGTCCTCGGGTGTCGACCGGTC   TCCCTCCCCTCCTCTCCT GTTCG-5'
	                                                       TGACACCCCACCTCCCCT
	                                                            Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 008-145493530
Forward Primer: 5'-GAGCCATTAAGGGCTAGAGGG-3' Tm: 59.378
Reverse Primer: 5'-GGAAAGCTCCCAAGAAGGGAAG-3' Tm: 59.258
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 135, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr8:145493634-145493691

Rank: 62	Homology Score: 5	RVD Match Score: 3.352	Left Half-Site RVD Score: 5.506	Right Half-Site RVD Score: 6.897
	Genomic Region: Intergenic	Closest Gene: SCXB
Match Type: L22R
	           Left Nuclease
	         TTTTCTGTCACCAATCCT
	5'-CCAAA TTTCCTCTCCCTAATCCT   GCAGGAGCCCACAGCTGGCCAG   AGGGAGGGGAGGAGAGGA CAAGC-3'
	3'-GGTTT AAAGGAGAGGGATTAGGA   CGTCCTCGGGTGTCGACCGGTC   TCCCTCCCCTCCTCTCCT GTTCG-5'
	                                                       TGACACCCCACCTCCCCT
	                                                            Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 008-145324445
Forward Primer: 5'-GAGCCATTAAGGGCTAGAGGG-3' Tm: 59.378
Reverse Primer: 5'-GGAAAGCTCCCAAGAAGGGAAG-3' Tm: 59.258
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 135, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr8:145324549-145324606

Rank: 63	Homology Score: 2	RVD Match Score: 3.353	Left Half-Site RVD Score: 5.987	Right Half-Site RVD Score: 6.417
	Genomic Region: Intergenic	Closest Gene: ARID3B
Match Type: L24R
	           Left Nuclease
	         TTTTCTGTCACCAATCCT
	5'-CTCCT CTTAGTTTCACCAATACT  TGACAGAGTCTGGCTCAGCACAGC  TCTATGGGATGGAGGGCC AGATG-3'
	3'-GAGGA GAATCAAAGTGGTTATGA  ACTGTCTCAGACCGAGTCGTGTCG  AGATACCCTACCTCCCGG TCTAC-5'
	                                                       TGACACCCCACCTCCCCT
	                                                            Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 015-074823089
Forward Primer: 5'-CACCTGTGTAGGTATCTGTGGAC-3' Tm: 59.148
Reverse Primer: 5'-CTGTCAGAGCAAAAGGGAGCAGG-3' Tm: 61.088
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 137, 133
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr15:74823194-74823253

Rank: 64	Homology Score: 2	RVD Match Score: 3.359	Left Half-Site RVD Score: 5.788	Right Half-Site RVD Score: 6.662
	Genomic Region: Intron	Closest Gene: ABCB7
Match Type: L16R
	           Left Nuclease
	         TTTTCTGTCACCAATCCT
	5'-CCTAA ATTGCTGACACCACCCCT      CCCCGACACCGCAGAA      GCAGTGGTGTGGTGTGGA GAGCA-3'
	3'-GGATT TAACGACTGTGGTGGGGA      GGGGCTGTGGCGTCTT      CGTCACCACACCACACCT CTCGT-5'
	                                                       TGACACCCCACCTCCCCT
	                                                            Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 00X-074326678
Forward Primer: 5'-CCCTTCCCTTCAAGGACACC-3' Tm: 59.508
Reverse Primer: 5'-GCTGAGTTTCGTCCTGTTTCTGG-3' Tm: 59.148
Expected PCR Product Length: 306
Expected CelI Cleavage Product Sizes: 173, 133
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chrX:74326823-74326874

Rank: 65	Homology Score: 5	RVD Match Score: 3.362	Left Half-Site RVD Score: 6.387	Right Half-Site RVD Score: 6.087
	Genomic Region: Intron	Closest Gene: DACH2
Match Type: R16L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-TATGA TCCCTGCCATCCAACAGT      TAGTGTATTTATATTC      ATGTTTAGTGACATAAAT AGCAA-3'
	3'-ATACT AGGGACGGTAGGTTGTCA      ATCACATAAATATAAG      TACAAATCACTGTATTTA TCGTT-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 00X-085476489
Forward Primer: 5'-GGAAATGTGAGGCCATCAGCTTTG-3' Tm: 59.038
Reverse Primer: 5'-CAGCCCCCAGAACTGTGAGA-3' Tm: 59.508
Expected PCR Product Length: 346
Expected CelI Cleavage Product Sizes: 194, 152
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chrX:85476655-85476706

Rank: 66	Homology Score: 5	RVD Match Score: 3.363	Left Half-Site RVD Score: 6.352	Right Half-Site RVD Score: 6.144
	Genomic Region: Intergenic	Closest Gene: SYT6
Match Type: L18R
	           Left Nuclease
	         TTTTCTGTCACCAATCCT
	5'-TTTCC CTTTCTGATAATAATCCT     ATAATGAACTCTAGGCAC     TCTGCTGGGTGGAGAGGA TATAA-3'
	3'-AAAGG GAAAGACTATTATTAGGA     TATTACTTGAGATCCGTG     AGACGACCCACCTCTCCT ATATT-5'
	                                                       TGACACCCCACCTCCCCT
	                                                            Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 001-114585589
Forward Primer: 5'-CCCCATAAATGATCTGCATCCAGG-3' Tm: 59.038
Reverse Primer: 5'-CGCTGAGAAGGCCAGATGAG-3' Tm: 59.508
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 135, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr1:114585695-114585748

Rank: 67	Homology Score: 5	RVD Match Score: 3.364	Left Half-Site RVD Score: 5.827	Right Half-Site RVD Score: 6.708
	Genomic Region: Intergenic	Closest Gene: C20orf197
Match Type: R16L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-GCACC TCCCATCAACCCCATAGC      AGGAATGGGGCGAACT      TTGATTGATGTAAGAAAA TGAAA-3'
	3'-CGTGG AGGGTAGTTGGGGTATCG      TCCTTACCCCGCTTGA      AACTAACTACATTCTTTT ACTTT-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 020-058618528
Forward Primer: 5'-GGGAGCTATGATCATGCCATAGG-3' Tm: 59.148
Reverse Primer: 5'-GCTCCATGTCATTGTTCTGGGC-3' Tm: 59.258
Expected PCR Product Length: 290
Expected CelI Cleavage Product Sizes: 135, 155
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr20:58618635-58618686

Rank: 68	Homology Score: 8	RVD Match Score: 3.364	Left Half-Site RVD Score: 6.153	Right Half-Site RVD Score: 6.349
	Genomic Region: Intergenic	Closest Gene: INADL
Match Type: R25L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-TCAAC TCCTCTCAACCCCAGAGC CATGTGTTACTCTTTGGGGAACTTC  AGGATAGCTGTCTGAAAA CAGTC-3'
	3'-AGTTG AGGAGAGTTGGGGTCTCG GTACACAATGAGAAACCCCTTGAAG  TCCTATCGACAGACTTTT GTCAG-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 001-062633493
Forward Primer: 5'-TCCTCCTGCCTAATGGCTTTACTG-3' Tm: 59.038
Reverse Primer: 5'-GCTACTGAGCCCAGCCTAATTTAC-3' Tm: 59.038
Expected PCR Product Length: 273
Expected CelI Cleavage Product Sizes: 130, 143
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr1:62633591-62633651

Rank: 69	Homology Score: 2	RVD Match Score: 3.366	Left Half-Site RVD Score: 6.792	Right Half-Site RVD Score: 5.769
	Genomic Region: Intron	Closest Gene: COPE
Match Type: L21R
	           Left Nuclease
	         TTTTCTGTCACCAATCCT
	5'-ACACA CTTTCTGTGACCAGTGCC   CTGGACATGGCCCGGAGTCAC    ATCCTAGGGTGGAGGGGG GCACC-3'
	3'-TGTGT GAAAGACACTGGTCACGG   GACCTGTACCGGGCCTCAGTG    TAGGATCCCACCTCCCCC CGTGG-5'
	                                                       TGACACCCCACCTCCCCT
	                                                            Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 019-019017199
Forward Primer: 5'-GGGATGGGAGCAGATCCACA-3' Tm: 59.508
Reverse Primer: 5'-AAGCCTTCTGTGTCTCGGCC-3' Tm: 59.508
Expected PCR Product Length: 273
Expected CelI Cleavage Product Sizes: 130, 143
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr19:19017299-19017355

Rank: 70	Homology Score: 2	RVD Match Score: 3.368	Left Half-Site RVD Score: 7.808	Right Half-Site RVD Score: 4.96
	Genomic Region: Intron	Closest Gene: GHRHR
Match Type: L21R
	           Left Nuclease
	         TTTTCTGTCACCAATCCT
	5'-AAACA TTTGCTGACATGGATCCT   GCTAGACATTGAGGGTGTGTG    TGTGTGGGGTGGGGGTGG GGGCC-3'
	3'-TTTGT AAACGACTGTACCTAGGA   CGATCTGTAACTCCCACACAC    ACACACCCCACCCCCACC CCCGG-5'
	                                                       TGACACCCCACCTCCCCT
	                                                            Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 007-031005868
Forward Primer: 5'-GTCAAAGTCTCTACTCTCTGACCC-3' Tm: 59.038
Reverse Primer: 5'-GGGAGAAGAGCATCTGGAACAGAA-3' Tm: 59.038
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 131, 139
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr7:31005969-31006025

Rank: 71	Homology Score: 2	RVD Match Score: 3.368	Left Half-Site RVD Score: 6.586	Right Half-Site RVD Score: 5.927
	Genomic Region: Intergenic	Closest Gene: LGALSL
Match Type: R26L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-CTGCC TCCCCTCCCCACAATATT TACAAGCATTGATTTTCTAGAAGGGA AGGGGTGGGGAGGGAAAA AGGAG-3'
	3'-GACGG AGGGGAGGGGTGTTATAA ATGTTCGTAACTAAAAGATCTTCCCT TCCCCACCCCTCCCTTTT TCCTC-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 002-064716657
Forward Primer: 5'-GAAATGCATCCCCTCTAGCCTG-3' Tm: 59.258
Reverse Primer: 5'-GCTGCATGGACAGTGCTCACA-3' Tm: 59.378
Expected PCR Product Length: 315
Expected CelI Cleavage Product Sizes: 131, 184
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr2:64716755-64716816

Rank: 72	Homology Score: 2	RVD Match Score: 3.369	Left Half-Site RVD Score: 5.906	Right Half-Site RVD Score: 6.618
	Genomic Region: Intergenic	Closest Gene: SALL4
Match Type: L14R
	           Left Nuclease
	         TTTTCTGTCACCAATCCT
	5'-AAAAA ATCTCTGTCGCCAAACCA       TCTGAACTCTAGTG       AAAGTGGGGTGGGTGTGA ACAAA-3'
	3'-TTTTT TAGAGACAGCGGTTTGGT       AGACTTGAGATCAC       TTTCACCCCACCCACACT TGTTT-5'
	                                                       TGACACCCCACCTCCCCT
	                                                            Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 020-050472318
Forward Primer: 5'-CTGGGTCATGTGGTACTGCATGT-3' Tm: 59.148
Reverse Primer: 5'-CTGCAGTTGATCCATCCAGACC-3' Tm: 59.258
Expected PCR Product Length: 285
Expected CelI Cleavage Product Sizes: 155, 130
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr20:50472446-50472495

Rank: 73	Homology Score: 2	RVD Match Score: 3.372	Left Half-Site RVD Score: 6.702	Right Half-Site RVD Score: 5.888
	Genomic Region: Intergenic	Closest Gene: LOC285593
Match Type: L14R
	           Left Nuclease
	         TTTTCTGTCACCAATCCT
	5'-GCAAG TTTTTTCCCGCCAATCCA       CCTGATAGCACAGA       GCTGTGTTGTGGAGGGCT TTAAA-3'
	3'-CGTTC AAAAAAGGGCGGTTAGGT       GGACTATCGTGTCT       CGACACAACACCTCCCGA AATTT-5'
	                                                       TGACACCCCACCTCCCCT
	                                                            Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 005-172898571
Forward Primer: 5'-GCGACAAGGAAATGGAGTCATGC-3' Tm: 59.148
Reverse Primer: 5'-GGCCTGTGTTTCAGAGTTGACTTC-3' Tm: 59.038
Expected PCR Product Length: 309
Expected CelI Cleavage Product Sizes: 163, 146
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr5:172898707-172898756

Rank: 74	Homology Score: 5	RVD Match Score: 3.376	Left Half-Site RVD Score: 7.052	Right Half-Site RVD Score: 5.536
	Genomic Region: Intron	Closest Gene: CACNG2
Match Type: R25L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-TCTCC TCCCCTCAACCTGAAAGC GAACACTCAAGGGAAGCTCTCCCCC  AGGATCTGTGACAGACAC TTTAG-3'
	3'-AGAGG AGGGGAGTTGGACTTTCG CTTGTGAGTTCCCTTCGAGAGGGGG  TCCTAGACACTGTCTGTG AAATC-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 022-037091993
Forward Primer: 5'-CTGCGAACTAACCTGGAAACGTAC-3' Tm: 59.038
Reverse Primer: 5'-GAGAGGAAGAGAGACTGAACGC-3' Tm: 59.258
Expected PCR Product Length: 364
Expected CelI Cleavage Product Sizes: 176, 188
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr22:37092137-37092197

Rank: 75	Homology Score: 2	RVD Match Score: 3.378	Left Half-Site RVD Score: 6.275	Right Half-Site RVD Score: 6.322
	Genomic Region: Intergenic	Closest Gene: USP9X
Match Type: L26R
	           Left Nuclease
	         TTTTCTGTCACCAATCCT
	5'-TCCAC TCTTCTGACACCACCCCA GAGAGGAGGGGGAGGAGTACTTTGTT ACTGCCTGGTGGAGGTGG ACATC-3'
	3'-AGGTG AGAAGACTGTGGTGGGGT CTCTCCTCCCCCTCCTCATGAAACAA TGACGGACCACCTCCACC TGTAG-5'
	                                                       TGACACCCCACCTCCCCT
	                                                            Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 00X-040907277
Forward Primer: 5'-CTGGTTAGGAGGGTTGAAGGTG-3' Tm: 59.258
Reverse Primer: 5'-GGTCAGAGAAGGCTGAGTAGG-3' Tm: 59.378
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 137, 133
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chrX:40907381-40907442

Rank: 76	Homology Score: 2	RVD Match Score: 3.378	Left Half-Site RVD Score: 6.235	Right Half-Site RVD Score: 6.365
	Genomic Region: Intergenic	Closest Gene: SOX1
Match Type: L19R
	           Left Nuclease
	         TTTTCTGTCACCAATCCT
	5'-GGCTG CTCTCTGTTACTAACCCT    CCTTTCACCTCCTCCTTTA     AATGTGAAGAGGAGGGGC TCCTA-3'
	3'-CCGAC GAGAGACAATGATTGGGA    GGAAAGTGGAGGAGGAAAT     TTACACTTCTCCTCCCCG AGGAT-5'
	                                                       TGACACCCCACCTCCCCT
	                                                            Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 013-112646719
Forward Primer: 5'-CTTGGCCTCTCTGAACACCG-3' Tm: 59.508
Reverse Primer: 5'-CATCCTGGTCCTGCACTTTTAGC-3' Tm: 59.148
Expected PCR Product Length: 302
Expected CelI Cleavage Product Sizes: 132, 170
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr13:112646822-112646876

Rank: 77	Homology Score: 2	RVD Match Score: 3.385	Left Half-Site RVD Score: 6.352	Right Half-Site RVD Score: 6.302
	Genomic Region: Intron	Closest Gene: C20orf112
Match Type: L26R
	           Left Nuclease
	         TTTTCTGTCACCAATCCT
	5'-TCTCC CTTTCTGTTAACAAACTT CAACAAAACTTACATTAGAGAATAAA AGTGTGGGCTGGGTGTGA TGGCT-3'
	3'-AGAGG GAAAGACAATTGTTTGAA GTTGTTTTGAATGTAATCTCTTATTT TCACACCCGACCCACACT ACCGA-5'
	                                                       TGACACCCCACCTCCCCT
	                                                            Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 020-031067101
Forward Primer: 5'-CTGCTCAGAGACCCAAGGTG-3' Tm: 59.508
Reverse Primer: 5'-TAGAGACAGCGTCTCACTGTGTTG-3' Tm: 59.038
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 135, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr20:31067203-31067264

Rank: 78	Homology Score: 2	RVD Match Score: 3.386	Left Half-Site RVD Score: 6.001	Right Half-Site RVD Score: 6.648
	Genomic Region: Intron	Closest Gene: C18orf1
Match Type: L25R
	           Left Nuclease
	         TTTTCTGTCACCAATCCT
	5'-AGCTA ATTTATGACACCAAGCAT ACTCAGTGCCTGCTGTGCACAGGTC  ACAGTGGGGTGGAGCCTT CCAGG-3'
	3'-TCGAT TAAATACTGTGGTTCGTA TGAGTCACGGACGACACGTGTCCAG  TGTCACCCCACCTCGGAA GGTCC-5'
	                                                       TGACACCCCACCTCCCCT
	                                                            Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 018-013545631
Forward Primer: 5'-GCAGTGCGGTTTCTTAGCTGCAA-3' Tm: 59.148
Reverse Primer: 5'-CAGCTCTGTGACTATGACTTCCC-3' Tm: 59.148
Expected PCR Product Length: 291
Expected CelI Cleavage Product Sizes: 135, 156
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr18:13545734-13545794

Rank: 79	Homology Score: 5	RVD Match Score: 3.389	Left Half-Site RVD Score: 6.267	Right Half-Site RVD Score: 6.409
	Genomic Region: Intergenic	Closest Gene: MIR802
Match Type: L21R
	           Left Nuclease
	         TTTTCTGTCACCAATCCT
	5'-CAGAG TCCTTTCCCACCAATCCT   TCATCAGCCTAATCAATACTA    GCAGTGGGGTGGAAGGAA GATGA-3'
	3'-GTCTC AGGAAAGGGTGGTTAGGA   AGTAGTCGGATTAGTTATGAT    CGTCACCCCACCTTCCTT CTACT-5'
	                                                       TGACACCCCACCTCCCCT
	                                                            Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 021-036773953
Forward Primer: 5'-CCAGAGCGGTTTTCCAAGATGC-3' Tm: 59.258
Reverse Primer: 5'-GGATTCACAACACCATGGATGGAC-3' Tm: 59.038
Expected PCR Product Length: 371
Expected CelI Cleavage Product Sizes: 175, 196
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr21:36774098-36774154

Rank: 80	Homology Score: 2	RVD Match Score: 3.39	Left Half-Site RVD Score: 6.144	Right Half-Site RVD Score: 6.562
	Genomic Region: Intergenic	Closest Gene: WDR92
Match Type: R15L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-ACTAG GCCCCACCTCTCAACAGT      ACCACACTGGGCATG       AGGTTTGGTGGGAACAAA CAAAC-3'
	3'-TGATC CGGGGTGGAGAGTTGTCA      TGGTGTGACCCGTAC       TCCAAACCACCCTTGTTT GTTTG-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 002-068331837
Forward Primer: 5'-GAGGAGAGGTGTCAGGCTCTT-3' Tm: 59.378
Reverse Primer: 5'-GATGAGTAGAGGCACATAGGTGAC-3' Tm: 59.038
Expected PCR Product Length: 316
Expected CelI Cleavage Product Sizes: 162, 154
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr2:68331972-68332022

Rank: 81	Homology Score: 5	RVD Match Score: 3.391	Left Half-Site RVD Score: 6.47	Right Half-Site RVD Score: 6.223
	Genomic Region: Intergenic	Closest Gene: SND1
Match Type: R16L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-ACCTC CCCCTCCCGCCCCAGAGT      TACGGGTTAAGGAGAA      AGACTTGGTCAGAGAAAA CGTGA-3'
	3'-TGGAG GGGGAGGGCGGGGTCTCA      ATGCCCAATTCCTCTT      TCTGAACCAGTCTCTTTT GCACT-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 007-127776243
Forward Primer: 5'-ATGACAGAAGGGGCGTGGTG-3' Tm: 59.508
Reverse Primer: 5'-AGTGGCGCCTCCTAGAAGCA-3' Tm: 59.508
Expected PCR Product Length: 292
Expected CelI Cleavage Product Sizes: 161, 131
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr7:127776376-127776427

Rank: 82	Homology Score: 5	RVD Match Score: 3.392	Left Half-Site RVD Score: 5.874	Right Half-Site RVD Score: 6.818
	Genomic Region: Intergenic	Closest Gene: MIR548I2
Match Type: L16R
	           Left Nuclease
	         TTTTCTGTCACCAATCCT
	5'-TTCTT TTCTCTGTCACTCATCCC      ACCCGACTAGAAATAC      CCACAGGGGTGGAGGGGC AGGCC-3'
	3'-AAGAA AAGAGACAGTGAGTAGGG      TGGGCTGATCTTTATG      GGTGTCCCCACCTCCCCG TCCGG-5'
	                                                       TGACACCCCACCTCCCCT
	                                                            Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 004-009669000
Forward Primer: 5'-ACTGAGGGAACTCAGAGGCC-3' Tm: 59.508
Reverse Primer: 5'-TGGGGATAAAGGAGGGGAGG-3' Tm: 59.508
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 137, 133
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr4:9669109-9669160

Rank: 83	Homology Score: 5	RVD Match Score: 3.392	Left Half-Site RVD Score: 6.417	Right Half-Site RVD Score: 6.288
	Genomic Region: Promoter	Closest Gene: CHAT
Match Type: R11L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-ACCTC TGCTCCCCACCCCATAGT        GCCAGCAGGAA         TGGGATGGTAACAGAAAG GGAAG-3'
	3'-TGGAG ACGAGGGGTGGGGTATCA        CGGTCGTCCTT         ACCCTACCATTGTCTTTC CCTTC-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 010-050821729
Forward Primer: 5'-GTGGGGGATGCTTTTGTCTGAG-3' Tm: 59.258
Reverse Primer: 5'-AAGTCTCTGCTCGCTGCCCT-3' Tm: 59.508
Expected PCR Product Length: 273
Expected CelI Cleavage Product Sizes: 130, 143
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr10:50821834-50821880

Rank: 84	Homology Score: 2	RVD Match Score: 3.393	Left Half-Site RVD Score: 5.342	Right Half-Site RVD Score: 7.53
	Genomic Region: Intron	Closest Gene: HDAC4
Match Type: R26L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-AATCA GACCCTTCACCCCACACC CACGAGAGCACCGAGGAACCAGGAGA AGGATACGTGAAACAAGA GAAGA-3'
	3'-TTAGT CTGGGAAGTGGGGTGTGG GTGCTCTCGTGGCTCCTTGGTCCTCT TCCTATGCACTTTGTTCT CTTCT-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 002-240313358
Forward Primer: 5'-CCAGAGCCATGGAACACAGG-3' Tm: 59.508
Reverse Primer: 5'-AGCGTGCCAGCTTCAGCACA-3' Tm: 59.508
Expected PCR Product Length: 275
Expected CelI Cleavage Product Sizes: 131, 144
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr2:240313456-240313517

Rank: 85	Homology Score: 2	RVD Match Score: 3.395	Left Half-Site RVD Score: 6.501	Right Half-Site RVD Score: 6.235
	Genomic Region: Intergenic	Closest Gene: IFLTD1
Match Type: L20R
	           Left Nuclease
	         TTTTCTGTCACCAATCCT
	5'-CCAAC TTTTCTCACACAAATTAT    CTCATTTAGTTTTCCGAATA    ACTCTCTGGGGGAGGGGG GAATT-3'
	3'-GGTTG AAAAGAGTGTGTTTAATA    GAGTAAATCAAAAGGCTTAT    TGAGAGACCCCCTCCCCC CTTAA-5'
	                                                       TGACACCCCACCTCCCCT
	                                                            Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 012-025903897
Forward Primer: 5'-CCAGCATCTTGTGGCTGAGTAG-3' Tm: 59.258
Reverse Primer: 5'-GGGAAGATGACAGTGTTCTTGACAGG-3' Tm: 60.578
Expected PCR Product Length: 290
Expected CelI Cleavage Product Sizes: 131, 159
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr12:25903997-25904052

Rank: 86	Homology Score: 5	RVD Match Score: 3.396	Left Half-Site RVD Score: 5.513	Right Half-Site RVD Score: 7.23
	Genomic Region: Intergenic	Closest Gene: CLINT1
Match Type: L15R
	           Left Nuclease
	         TTTTCTGTCACCAATCCT
	5'-TGCAC TTTTCAGCCCCCACTCCT      CCTTTATGAAGAGAA       CCTGTGTGCTGGTGGGAA GAGCT-3'
	3'-ACGTG AAAAGTCGGGGGTGAGGA      GGAAATACTTCTCTT       GGACACACGACCACCCTT CTCGA-5'
	                                                       TGACACCCCACCTCCCCT
	                                                            Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 005-157580667
Forward Primer: 5'-GTAATCCAGGGTCTCTGTTTGTCC-3' Tm: 59.038
Reverse Primer: 5'-GTCTTTGGGTGGCATTTGTGAGG-3' Tm: 59.148
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 133, 137
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr5:157580773-157580823

Rank: 87	Homology Score: 2	RVD Match Score: 3.396	Left Half-Site RVD Score: 6.831	Right Half-Site RVD Score: 5.895
	Genomic Region: Intron	Closest Gene: CCDC51
Match Type: R15L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-GGTTC TTCCCACCACGCTACACT      ACTACATGCCTAAGC       AGGAGTGGGAACAGAGAT GACAG-3'
	3'-CCAAG AAGGGTGGTGCGATGTGA      TGATGTACGGATTCG       TCCTCACCCTTGTCTCTA CTGTC-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 003-048475638
Forward Primer: 5'-GTGGCCTCCAGTTTATAAACCCAG-3' Tm: 59.038
Reverse Primer: 5'-GCTGCGGTACTGTTACTGTGTG-3' Tm: 59.258
Expected PCR Product Length: 293
Expected CelI Cleavage Product Sizes: 134, 159
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr3:48475745-48475795

Rank: 88	Homology Score: 2	RVD Match Score: 3.396	Left Half-Site RVD Score: 7.07	Right Half-Site RVD Score: 5.74
	Genomic Region: Intergenic	Closest Gene: ZNRF2
Match Type: L12R
	           Left Nuclease
	         TTTTCTGTCACCAATCCT
	5'-TCCCA TCCTCTGTCATCTATCCC        TACAATCTGCAG        CCTGTGTGGTGTGGGGGG CAGGA-3'
	3'-AGGGT AGGAGACAGTAGATAGGG        ATGTTAGACGTC        GGACACACCACACCCCCC GTCCT-5'
	                                                       TGACACCCCACCTCCCCT
	                                                            Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 007-030310150
Forward Primer: 5'-CTGCTGCCAAAAGCTACCCATG-3' Tm: 59.258
Reverse Primer: 5'-GAGCTCCAGTTTCCTGCACTGT-3' Tm: 59.258
Expected PCR Product Length: 386
Expected CelI Cleavage Product Sizes: 130, 256
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr7:30310251-30310298

Rank: 89	Homology Score: 2	RVD Match Score: 3.401	Left Half-Site RVD Score: 7.533	Right Half-Site RVD Score: 5.395
	Genomic Region: Intergenic	Closest Gene: IQUB
Match Type: L24R
	           Left Nuclease
	         TTTTCTGTCACCAATCCT
	5'-TGTTT TCTTCTGTCGTCCATGCT  GTAAGCTTTGTAGAACCCTAGCCT  TGTGTTGGGAGGAGGGGG AAGAG-3'
	3'-ACAAA AGAAGACAGCAGGTACGA  CATTCGAAACATCTTGGGATCGGA  ACACAACCCTCCTCCCCC TTCTC-5'
	                                                       TGACACCCCACCTCCCCT
	                                                            Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 007-123033818
Forward Primer: 5'-CTACCCCTATGCAGGTTTGCTGA-3' Tm: 59.148
Reverse Primer: 5'-CTTCCCCCAACTCTCACAGG-3' Tm: 59.508
Expected PCR Product Length: 311
Expected CelI Cleavage Product Sizes: 155, 156
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr7:123033941-123034000

Rank: 90	Homology Score: 5	RVD Match Score: 3.406	Left Half-Site RVD Score: 6.265	Right Half-Site RVD Score: 6.54
	Genomic Region: Intron	Closest Gene: ARID1B
Match Type: L22R
	           Left Nuclease
	         TTTTCTGTCACCAATCCT
	5'-AGGGT CTTTCTGAAATCAAGCCT   TGCAAGTGATGTTTCCAAGGAG   GCTGTCGGCTGGAGCGGA GCCTG-3'
	3'-TCCCA GAAAGACTTTAGTTCGGA   ACGTTCACTACAAAGGTTCCTC   CGACAGCCGACCTCGCCT CGGAC-5'
	                                                       TGACACCCCACCTCCCCT
	                                                            Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 006-157508829
Forward Primer: 5'-GCCAGCCTCCTCTATTAATGGAGT-3' Tm: 59.038
Reverse Primer: 5'-GAGTAAAGGAAGAAAGCCGTCTTTTGCAG-3' Tm: 60.178
Expected PCR Product Length: 320
Expected CelI Cleavage Product Sizes: 178, 142
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr6:157508976-157509033

Rank: 91	Homology Score: 2	RVD Match Score: 3.409	Left Half-Site RVD Score: 6.349	Right Half-Site RVD Score: 6.482
	Genomic Region: Intron	Closest Gene: CCDC64
Match Type: L11R
	           Left Nuclease
	         TTTTCTGTCACCAATCCT
	5'-GGCAT CCCTCTGTCACCAGTGCT        GAGACCCCTGG         ACTGTGGTGGTGATGTGA GATCC-3'
	3'-CCGTA GGGAGACAGTGGTCACGA        CTCTGGGGACC         TGACACCACCACTACACT CTAGG-5'
	                                                       TGACACCCCACCTCCCCT
	                                                            Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 012-120471484
Forward Primer: 5'-GCCGGCTGAGAGTTCTTGCT-3' Tm: 59.508
Reverse Primer: 5'-GTATGCCTGTATCTGGCTGTGTTG-3' Tm: 59.038
Expected PCR Product Length: 359
Expected CelI Cleavage Product Sizes: 224, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr12:120471683-120471729

Rank: 92	Homology Score: 2	RVD Match Score: 3.41	Left Half-Site RVD Score: 6.246	Right Half-Site RVD Score: 6.603
	Genomic Region: Intron	Closest Gene: MEGF11
Match Type: R16L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-CCAGT TCCCCTCCTCACTCCAAT      CCCCAGCATCACTGTG      AGGAATGGGGACAACAAG GACAG-3'
	3'-GGTCA AGGGGAGGAGTGAGGTTA      GGGGTCGTAGTGACAC      TCCTTACCCCTGTTGTTC CTGTC-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 015-066339107
Forward Primer: 5'-CACACAGTAGGCTTTGCTGGC-3' Tm: 59.378
Reverse Primer: 5'-GGTGAACTTCATGGGATGTGCTG-3' Tm: 59.148
Expected PCR Product Length: 274
Expected CelI Cleavage Product Sizes: 131, 143
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr15:66339210-66339261

Rank: 93	Homology Score: 2	RVD Match Score: 3.41	Left Half-Site RVD Score: 5.997	Right Half-Site RVD Score: 6.886
	Genomic Region: Promoter	Closest Gene: ODF4
Match Type: R20L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-TCACC TCCCATCACCACCACACT    GGGGAGCAAATTTCAGCATG    AGGTTTGGTGAAGACAAA TTAAC-3'
	3'-AGTGG AGGGTAGTGGTGGTGTGA    CCCCTCGTTTAAAGTCGTAC    TCCAAACCACTTCTGTTT AATTG-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 017-008242168
Forward Primer: 5'-CAGCTATTGCGGGGAGCTAATG-3' Tm: 59.258
Reverse Primer: 5'-AGGCCACAGCTCAGAACTGTGGA-3' Tm: 61.088
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 136, 134
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr17:8242274-8242329

Rank: 94	Homology Score: 5	RVD Match Score: 3.411	Left Half-Site RVD Score: 7.369	Right Half-Site RVD Score: 5.492
	Genomic Region: Intergenic	Closest Gene: SMAD3
Match Type: R13L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-TGTTC TTCCACCCAGCCCTCAGT       GCTCTGCTGAGGC        AGGATGGGTGGCTGGAAA AATGG-3'
	3'-ACAAG AAGGTGGGTCGGGAGTCA       CGAGACGACTCCG        TCCTACCCACCGACCTTT TTACC-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 015-067305574
Forward Primer: 5'-CCCCACCTTGCTGAATGGAC-3' Tm: 59.508
Reverse Primer: 5'-CCAGTCCTTGATCTCTTCCTGAGT-3' Tm: 59.038
Expected PCR Product Length: 275
Expected CelI Cleavage Product Sizes: 142, 133
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr15:67305690-67305738

Rank: 95	Homology Score: 2	RVD Match Score: 3.411	Left Half-Site RVD Score: 6.057	Right Half-Site RVD Score: 6.826
	Genomic Region: Intergenic	Closest Gene: CREM
Match Type: R24L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-ATGTC CCTGCCCCACCCCCCAGT  TTCAAGAGTGGATCTGATCAGTGT  GTGGTGGGAGACAGAAAA TTGGG-3'
	3'-TACAG GGACGGGGTGGGGGGTCA  AAGTTCTCACCTAGACTAGTCACA  CACCACCCTCTGTCTTTT AACCC-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 010-035501885
Forward Primer: 5'-CAGGATGCTGTGTCCCCTCA-3' Tm: 59.508
Reverse Primer: 5'-GAGCTCCGACTCTGAAGGAAG-3' Tm: 59.378
Expected PCR Product Length: 303
Expected CelI Cleavage Product Sizes: 131, 172
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr10:35501984-35502043

Rank: 96	Homology Score: 2	RVD Match Score: 3.412	Left Half-Site RVD Score: 6.479	Right Half-Site RVD Score: 6.371
	Genomic Region: Intron	Closest Gene: ADCK2
Match Type: R24L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-TGGGC CCGTCTCCAGCCCACAGA  AAGGCTTCAGTCTGGAGAGAGGAT  GGGATTTGTGTCTGTAAA ACTGT-3'
	3'-ACCCG GGCAGAGGTCGGGTGTCT  TTCCGAAGTCAGACCTCTCTCCTA  CCCTAAACACAGACATTT TGACA-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 007-140379038
Forward Primer: 5'-TCTGCGCCCCTTTGTCACCA-3' Tm: 59.508
Reverse Primer: 5'-CCTCTAAGGCAGGTCACCAAC-3' Tm: 59.378
Expected PCR Product Length: 299
Expected CelI Cleavage Product Sizes: 132, 167
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr7:140379138-140379197

Rank: 97	Homology Score: 2	RVD Match Score: 3.412	Left Half-Site RVD Score: 6.17	Right Half-Site RVD Score: 6.707
	Genomic Region: Intergenic	Closest Gene: MIR4643
Match Type: R11L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-GACCC TCCGCACCACCCCTCAAC        ACCTCTCCCAT         ATGAGGGGTGACAGCTAA TTGTT-3'
	3'-CTGGG AGGCGTGGTGGGGAGTTG        TGGAGAGGGTA         TACTCCCCACTGTCGATT AACAA-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 006-092651079
Forward Primer: 5'-CAGCAAGGAGGCCAAGAACTCA-3' Tm: 59.258
Reverse Primer: 5'-GTGTGTGGGCCTCAAGTTTTGTAC-3' Tm: 59.038
Expected PCR Product Length: 352
Expected CelI Cleavage Product Sizes: 151, 201
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr6:92651205-92651251

Rank: 98	Homology Score: 10	RVD Match Score: 3.414	Left Half-Site RVD Score: 5.315	Right Half-Site RVD Score: 7.738
	Genomic Region: Intergenic	Closest Gene: FOXP1
Match Type: R20L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-TGAAA TGCCCCCCACCCCAGAGT    ATATTCATTAGGACTCTTTG    ATTATAAGAGACAGAAAA TCAAC-3'
	3'-ACTTT ACGGGGGGTGGGGTCTCA    TATAAGTAATCCTGAGAAAC    TAATATTCTCTGTCTTTT AGTTG-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 003-070995805
Forward Primer: 5'-GCCGTTGCTTTTGCCTTGAACATC-3' Tm: 59.038
Reverse Primer: 5'-TACTTGAGTCCCTGGATCTGGC-3' Tm: 59.258
Expected PCR Product Length: 306
Expected CelI Cleavage Product Sizes: 176, 130
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr3:70995951-70996006

Rank: 99	Homology Score: 10	RVD Match Score: 3.415	Left Half-Site RVD Score: 5.371	Right Half-Site RVD Score: 7.681
	Genomic Region: Intron	Closest Gene: MYT1L
Match Type: R15L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-GAATG TTCCCTCCAACCCAGAGT      GCTGAGAAGGCTGGC       AGGATAGAGGCCAGAACA TGTCT-3'
	3'-CTTAC AAGGGAGGTTGGGTCTCA      CGACTCTTCCGACCG       TCCTATCTCCGGTCTTGT ACAGA-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 002-001827851
Forward Primer: 5'-GGGGGTTGGGGAACGTTTTTAG-3' Tm: 59.258
Reverse Primer: 5'-TCAGGACGAGAGCAGCTCAAATG-3' Tm: 59.148
Expected PCR Product Length: 446
Expected CelI Cleavage Product Sizes: 315, 131
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr2:1828139-1828189

Rank: 100	Homology Score: 5	RVD Match Score: 3.418	Left Half-Site RVD Score: 6.211	Right Half-Site RVD Score: 6.677
	Genomic Region: Intron	Closest Gene: KIAA1217
Match Type: L20R
	           Left Nuclease
	         TTTTCTGTCACCAATCCT
	5'-GACAT TTTTCTTTTATCCATCCT    ACAGCACTGCACAATTATTG    ATTGTGGCTTGGAAGCGA AATGA-3'
	3'-CTGTA AAAAGAAAATAGGTAGGA    TGTCGTGACGTGTTAATAAC    TAACACCGAACCTTCGCT TTACT-5'
	                                                       TGACACCCCACCTCCCCT
	                                                            Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 010-024751913
Forward Primer: 5'-GGTGGATGTGCAGAGATGGAG-3' Tm: 59.378
Reverse Primer: 5'-CTCTACTGAGCCCTCCAGTG-3' Tm: 59.508
Expected PCR Product Length: 343
Expected CelI Cleavage Product Sizes: 208, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr10:24752091-24752146

Plate format Oligo Order Form
Well
Name
Sequence
5' mods
Internal
Notes
Primer Plate #1
A1
019-055627003F
CCAGGAAATGGGGGTGTGTC
Hockemeyer_AAVS1_Ranking-RVDs	Primer pair generated using optimal parameters. 
A2
019-055627003R
GTCCACTTCAGGACAGCATGTTTG
Hockemeyer_AAVS1_Ranking-RVDs	Primer pair generated using optimal parameters. 
B1
016-024267933F
CAAAGTCCCTGTGGATGCTGACAA
Hockemeyer_AAVS1_Ranking-RVDs	Primer pair generated using optimal parameters. 
B2
016-024267933R
TAGTCCTGCACACACCTCTGG
Hockemeyer_AAVS1_Ranking-RVDs	Primer pair generated using optimal parameters. 
C1
011-109627678F
TCCTCCTTGGAACCCCAGATTTG
Hockemeyer_AAVS1_Ranking-RVDs	Primer pair generated using optimal parameters. 
C2
011-109627678R
TGTCTTCACTGCTGCACATTCCC
Hockemeyer_AAVS1_Ranking-RVDs	Primer pair generated using optimal parameters. 
D1
003-051729704F
GGAGATGAACTCAGGCTGAAGTG
Hockemeyer_AAVS1_Ranking-RVDs	Primer pair generated using optimal parameters. 
D2
003-051729704R
TTCCCTCCCACCTGGGTCTT
Hockemeyer_AAVS1_Ranking-RVDs	Primer pair generated using optimal parameters. 
E1
007-115754830F
CGGTTGAGACTGAAGCATGCAAAG
Hockemeyer_AAVS1_Ranking-RVDs	Primer pair generated using optimal parameters. 
E2
007-115754830R
AAGGACCACACTACTGACATTAGATCTACC
Hockemeyer_AAVS1_Ranking-RVDs	Primer pair generated using optimal parameters. 
F1
006-138655843F
GGCTTCTCTGGGAAAGAAACCCCT
Hockemeyer_AAVS1_Ranking-RVDs	Primer pair generated using optimal parameters. 
F2
006-138655843R
TTTGGGGAACGCGGACAGGT
Hockemeyer_AAVS1_Ranking-RVDs	Primer pair generated using optimal parameters. 
G1
020-025439956F
AGTCCCTGCACTGACTCAGC
Hockemeyer_AAVS1_Ranking-RVDs	Primer pair generated using optimal parameters. 
G2
020-025439956R
GTGCTTGATGCGTGGGAGTC
Hockemeyer_AAVS1_Ranking-RVDs	Primer pair generated using optimal parameters. 
H1
001-024697363F
AGCCTCCCAAAGTGCTAGGAC
Hockemeyer_AAVS1_Ranking-RVDs	Primer pair generated using optimal parameters. 
H2
001-024697363R
GTGGAGAACATCCATTCACAGGG
Hockemeyer_AAVS1_Ranking-RVDs	Primer pair generated using optimal parameters. 
A3
016-009446101F
ATCCTTCCTCTGGGGGCACT
Hockemeyer_AAVS1_Ranking-RVDs	Primer pair generated using optimal parameters. 
A4
016-009446101R
CTCCAACCTCCCAATGAAACACC
Hockemeyer_AAVS1_Ranking-RVDs	Primer pair generated using optimal parameters. 
B3
012-047346370F
CTTGGTATGTTCCAGTGTGGCC
Hockemeyer_AAVS1_Ranking-RVDs	Primer pair generated using optimal parameters. 
B4
012-047346370R
GGATGTGTGCAGGATGTACAGG
Hockemeyer_AAVS1_Ranking-RVDs	Primer pair generated using optimal parameters. 
C3
00X-031479613F
GTCTGGACATGTCTGAATGCCTAG
Hockemeyer_AAVS1_Ranking-RVDs	Primer pair generated using optimal parameters. 
C4
00X-031479613R
GGTTAGGTAAAGTGTCCCCTGC
Hockemeyer_AAVS1_Ranking-RVDs	Primer pair generated using optimal parameters. 
D3
003-159874261F
GCCCAGCTCTGAAAAGGACTAAG
Hockemeyer_AAVS1_Ranking-RVDs	Primer pair generated using optimal parameters. 
D4
003-159874261R
GCTCCACTGAATATCCCCCAAG
Hockemeyer_AAVS1_Ranking-RVDs	Primer pair generated using optimal parameters. 
E3
003-151589613F
GGCTACAGTCTAGATGACGTGTTC
Hockemeyer_AAVS1_Ranking-RVDs	Primer pair generated using optimal parameters. 
E4
003-151589613R
CGACTCTGATTCTTGACTGCCTAC
Hockemeyer_AAVS1_Ranking-RVDs	Primer pair generated using optimal parameters. 
F3
002-207954053F
GTTTTAGCCACAGGGAAGCTGC
Hockemeyer_AAVS1_Ranking-RVDs	Primer pair generated using optimal parameters. 
F4
002-207954053R
GAAGCTGATCCCAAGAAGTTTGGC
Hockemeyer_AAVS1_Ranking-RVDs	Primer pair generated using optimal parameters. 
G3
00X-018438373F
CAGGTCATGACCATCAGTGATCAG
Hockemeyer_AAVS1_Ranking-RVDs	Primer pair generated using optimal parameters. 
G4
00X-018438373R
CACCTGTCAGGCTTGTGGCT
Hockemeyer_AAVS1_Ranking-RVDs	Primer pair generated using optimal parameters. 
H3
009-073729326F
CCACATGCTCAAGAGCCAGG
Hockemeyer_AAVS1_Ranking-RVDs	Primer pair generated using optimal parameters. 
H4
009-073729326R
GGCATGTCCCTTAGATTCCAAGGT
Hockemeyer_AAVS1_Ranking-RVDs	Primer pair generated using optimal parameters. 
A5
011-057225300F
AGCCTCACCGTACAGATGAGGA
Hockemeyer_AAVS1_Ranking-RVDs	Primer pair generated using optimal parameters. 
A6
011-057225300R
TTTAGGTGGCTGGCTGGTGG
Hockemeyer_AAVS1_Ranking-RVDs	Primer pair generated using optimal parameters. 
B5
003-015883800F
GCCAGGCACAAAAAGACAAAGTTTGCATG
Hockemeyer_AAVS1_Ranking-RVDs	Primer pair generated using optimal parameters. 
B6
003-015883800R
GGGGTATCCATCACCTCAAGC
Hockemeyer_AAVS1_Ranking-RVDs	Primer pair generated using optimal parameters. 
C5
006-160296301F
CTGGTGAGCTTTCCCTGTGTAC
Hockemeyer_AAVS1_Ranking-RVDs	Primer pair generated using optimal parameters. 
C6
006-160296301R
GAACTCATCTAGTCTCCCCGG
Hockemeyer_AAVS1_Ranking-RVDs	Primer pair generated using optimal parameters. 
D5
001-202164461F
TGCTCAGTGTTGGGAGTGTCCT
Hockemeyer_AAVS1_Ranking-RVDs	Primer pair generated using optimal parameters. 
D6
001-202164461R
TTTGCCAGATGCTGAGCGCC
Hockemeyer_AAVS1_Ranking-RVDs	Primer pair generated using optimal parameters. 
E5
005-127879056F
CTCCTGGATAGTGTCAGAGAAAGC
Hockemeyer_AAVS1_Ranking-RVDs	Primer pair generated using optimal parameters. 
E6
005-127879056R
CTGCTCCCAATGTAGTTTCCCATG
Hockemeyer_AAVS1_Ranking-RVDs	Primer pair generated using optimal parameters. 
F5
002-154289086F
GGTTTCAAGTTTCACCTATTGCTTGGCAG
Hockemeyer_AAVS1_Ranking-RVDs	Primer pair generated using optimal parameters. 
F6
002-154289086R
CGTGTTATGTGTGTTTAGCCCCC
Hockemeyer_AAVS1_Ranking-RVDs	Primer pair generated using optimal parameters. 
G5
001-037420737F
CACTGATTTGCTGAGGCTCAGATG
Hockemeyer_AAVS1_Ranking-RVDs	Primer pair generated using optimal parameters. 
G6
001-037420737R
GCCTCTGTGTATCCATCCACG
Hockemeyer_AAVS1_Ranking-RVDs	Primer pair generated using optimal parameters. 
H5
017-035084330F
GAGCCTCTGCTGGTTGGATG
Hockemeyer_AAVS1_Ranking-RVDs	Primer pair generated using optimal parameters. 
H6
017-035084330R
ATGTTGGGTGCTGGGGAAGG
Hockemeyer_AAVS1_Ranking-RVDs	Primer pair generated using optimal parameters. 
A7
008-107822970F
GGCTAAGTGTGAGAATCGAGTGAG
Hockemeyer_AAVS1_Ranking-RVDs	Primer pair generated using optimal parameters. 
A8
008-107822970R
GTTAGTGAGACAGCATGTCCCTTC
Hockemeyer_AAVS1_Ranking-RVDs	Primer pair generated using optimal parameters. 
B7
006-168932997F
TCCTTCGTCCTGGGATTGGG
Hockemeyer_AAVS1_Ranking-RVDs	Primer pair generated using optimal parameters. 
B8
006-168932997R
GGGTGCACAGTTACAGAGGAG
Hockemeyer_AAVS1_Ranking-RVDs	Primer pair generated using optimal parameters. 
C7
014-054175647F
GCCAGAACAGCCTTGTGGATAC
Hockemeyer_AAVS1_Ranking-RVDs	Primer pair generated using optimal parameters. 
C8
014-054175647R
GACAGGATGGGACGCTGATAG
Hockemeyer_AAVS1_Ranking-RVDs	Primer pair generated using optimal parameters. 
D7
011-040808649F
CCCATGTCTCAGTCTCTGTTCTAC
Hockemeyer_AAVS1_Ranking-RVDs	Primer pair generated using optimal parameters. 
D8
011-040808649R
CTCACTTGTCAGCAAGCACAAAGG
Hockemeyer_AAVS1_Ranking-RVDs	Primer pair generated using optimal parameters. 
E7
016-074868261F
TCAAGAATCAGGATGTCAGGGGAG
Hockemeyer_AAVS1_Ranking-RVDs	Primer pair generated using optimal parameters. 
E8
016-074868261R
CTACTGGAAAGTTCTACTGGACGG
Hockemeyer_AAVS1_Ranking-RVDs	Primer pair generated using optimal parameters. 
F7
016-067073560F
GAGTGATAGAACTATGCAGGCTATCAGG
Hockemeyer_AAVS1_Ranking-RVDs	Primer pair generated using optimal parameters. 
F8
016-067073560R
GCACGGAACAGTACAGTACGAG
Hockemeyer_AAVS1_Ranking-RVDs	Primer pair generated using optimal parameters. 
G7
008-110449611F
GTGCACAACGTGCAGGTTAGTTAC
Hockemeyer_AAVS1_Ranking-RVDs	Primer pair generated using optimal parameters. 
G8
008-110449611R
CCTGCAGATTGCAAATACATAATACCCCC
Hockemeyer_AAVS1_Ranking-RVDs	Primer pair generated using optimal parameters. 
H7
019-038858026F
TTCCTCTCCCCGCAGACTAC
Hockemeyer_AAVS1_Ranking-RVDs	Primer pair generated using optimal parameters. 
H8
019-038858026R
AGTGCACACAGACTGGCCTC
Hockemeyer_AAVS1_Ranking-RVDs	Primer pair generated using optimal parameters. 
A9
013-103511881F
TCTCTGGCTGGCAGTTGAGG
Hockemeyer_AAVS1_Ranking-RVDs	Primer pair generated using optimal parameters. 
A10
013-103511881R
CAGGTCACTGAAAACTGCAAACCG
Hockemeyer_AAVS1_Ranking-RVDs	Primer pair generated using optimal parameters. 
B9
003-124702567F
TGCAAGTCTACAACCAGGGTGTG
Hockemeyer_AAVS1_Ranking-RVDs	Primer pair generated using optimal parameters. 
B10
003-124702567R
CAGAAGGCAGCTGGAAGTCTC
Hockemeyer_AAVS1_Ranking-RVDs	Primer pair generated using optimal parameters. 
C9
001-201121271F
GAGGGATGAGGGGAAATGGG
Hockemeyer_AAVS1_Ranking-RVDs	Primer pair generated using optimal parameters. 
C10
001-201121271R
GGAGCTGTTTGGGTAGATCTATGG
Hockemeyer_AAVS1_Ranking-RVDs	Primer pair generated using optimal parameters. 
D9
005-040515613F
GTTGAAAAGTGGGGAAAGGGGG
Hockemeyer_AAVS1_Ranking-RVDs	Primer pair generated using optimal parameters. 
D10
005-040515613R
CTTTAGGACCACTCCTGAATGGG
Hockemeyer_AAVS1_Ranking-RVDs	Primer pair generated using optimal parameters. 
E9
016-027592598F
CCCTGTGCACATAGGAATAGCC
Hockemeyer_AAVS1_Ranking-RVDs	Primer pair generated using optimal parameters. 
E10
016-027592598R
GGGTGATGGGTGCATCAATATCTC
Hockemeyer_AAVS1_Ranking-RVDs	Primer pair generated using optimal parameters. 
F9
002-155321775F
TACCCCCCACCCCCTTGATA
Hockemeyer_AAVS1_Ranking-RVDs	Primer pair generated using optimal parameters. 
F10
002-155321775R
GAAGGTGGTGTCACTTGTCTTCC
Hockemeyer_AAVS1_Ranking-RVDs	Primer pair generated using optimal parameters. 
G9
020-019256133F
GGAAGGGTTTGCAAGCCCTG
Hockemeyer_AAVS1_Ranking-RVDs	Primer pair generated using optimal parameters. 
G10
020-019256133R
AAGCAAGCCGCATGCACTAAGG
Hockemeyer_AAVS1_Ranking-RVDs	Primer pair generated using optimal parameters. 
H9
002-084649832F
AAAGAATGTGGGCTCTGGATGGG
Hockemeyer_AAVS1_Ranking-RVDs	Primer pair generated using optimal parameters. 
H10
002-084649832R
GAGGCTGTAACAGTGTCAACGTG
Hockemeyer_AAVS1_Ranking-RVDs	Primer pair generated using optimal parameters. 
A11
001-226315854F
AAGCGCCCTCAGCAACCTCA
Hockemeyer_AAVS1_Ranking-RVDs	Primer pair generated using optimal parameters. 
A12
001-226315854R
ATTTAGCCAACCGTGGTGGCAG
Hockemeyer_AAVS1_Ranking-RVDs	Primer pair generated using optimal parameters. 
B11
011-011075984F
GCCTCATTACTGCTAGGTGGAAG
Hockemeyer_AAVS1_Ranking-RVDs	Primer pair generated using optimal parameters. 
B12
011-011075984R
CACCCTAACATCCAGGCATGATGA
Hockemeyer_AAVS1_Ranking-RVDs	Primer pair generated using optimal parameters. 
C11
010-101836357F
CAGCCGCTAATGATCCCAGTTG
Hockemeyer_AAVS1_Ranking-RVDs	Primer pair generated using optimal parameters. 
C12
010-101836357R
GACATAGCAGGTGCCTTCCTATTC
Hockemeyer_AAVS1_Ranking-RVDs	Primer pair generated using optimal parameters. 
D11
001-178005473F
CTCACAGAAGTGCGAACCGTATTG
Hockemeyer_AAVS1_Ranking-RVDs	Primer pair generated using optimal parameters. 
D12
001-178005473R
CACCAATATGGCCACCTCTGTAC
Hockemeyer_AAVS1_Ranking-RVDs	Primer pair generated using optimal parameters. 
E11
017-063492561F
CTCTGTTCCCCACTGATCACC
Hockemeyer_AAVS1_Ranking-RVDs	Primer pair generated using optimal parameters. 
E12
017-063492561R
AGCATGGAAGGTCCCCCACA
Hockemeyer_AAVS1_Ranking-RVDs	Primer pair generated using optimal parameters. 
F11
004-146944903F
CAGAGGAGTTCAGCTCAGTTTTGC
Hockemeyer_AAVS1_Ranking-RVDs	Primer pair generated using optimal parameters. 
F12
004-146944903R
CCCTCCCCCAAGGACAACAA
Hockemeyer_AAVS1_Ranking-RVDs	Primer pair generated using optimal parameters. 
G11
011-119574363F
CAACGAGGCTGACGCTCTTTC
Hockemeyer_AAVS1_Ranking-RVDs	Primer pair generated using optimal parameters. 
G12
011-119574363R
CTGAGCACCTGTGTGCCAGT
Hockemeyer_AAVS1_Ranking-RVDs	Primer pair generated using optimal parameters. 
H11
006-099703892F
GGACTGCATAAACCACTTGAGCC
Hockemeyer_AAVS1_Ranking-RVDs	Primer pair generated using optimal parameters. 
H12
006-099703892R
GCAGGACTCTCTAGCTGGCTAT
Hockemeyer_AAVS1_Ranking-RVDs	Primer pair generated using optimal parameters. 
Primer Plate #2
A1
007-069107597F
GATGTCAGTGTCCTCAGCTGTTAG
Hockemeyer_AAVS1_Ranking-RVDs	Primer pair generated using optimal parameters. 
A2
007-069107597R
CCTGACTCACAGAAACTGTGGG
Hockemeyer_AAVS1_Ranking-RVDs	Primer pair generated using optimal parameters. 
B1
006-011853744F
GTCTACGGATCTAACACTCCAAAAATCTTAGC
Hockemeyer_AAVS1_Ranking-RVDs	Warning! Primer pair was generated using suboptimal parameters. 
B2
006-011853744R
CTAGGGGGCTGAAATGGAACAAC
Hockemeyer_AAVS1_Ranking-RVDs	Warning! Primer pair was generated using suboptimal parameters. 
C1
001-206729981F
CTCTCTAGGTTGCCATAACTCTGC
Hockemeyer_AAVS1_Ranking-RVDs	Primer pair generated using optimal parameters. 
C2
001-206729981R
TCTCATCAGCCCCGGTGACT
Hockemeyer_AAVS1_Ranking-RVDs	Primer pair generated using optimal parameters. 
D1
004-148942680F
GTAGGTAAGCCAGGGAGCTGT
Hockemeyer_AAVS1_Ranking-RVDs	Primer pair generated using optimal parameters. 
D2
004-148942680R
CCTGCCAACACCATGTTTCCAC
Hockemeyer_AAVS1_Ranking-RVDs	Primer pair generated using optimal parameters. 
E1
011-116565112F
CATCTATGCCCCACCAAGAGG
Hockemeyer_AAVS1_Ranking-RVDs	Primer pair generated using optimal parameters. 
E2
011-116565112R
ACATCTGTGGCAGGTGGAGC
Hockemeyer_AAVS1_Ranking-RVDs	Primer pair generated using optimal parameters. 
F1
00X-074326678F
CCCTTCCCTTCAAGGACACC
Hockemeyer_AAVS1_Ranking-RVDs	Primer pair generated using optimal parameters. 
F2
00X-074326678R
GCTGAGTTTCGTCCTGTTTCTGG
Hockemeyer_AAVS1_Ranking-RVDs	Primer pair generated using optimal parameters. 
G1
021-020928381F
GGTGAACTGGGGTCCCAAGTTT
Hockemeyer_AAVS1_Ranking-RVDs	Primer pair generated using optimal parameters. 
G2
021-020928381R
CTGAGGGCCTACTCATCTTTCTTC
Hockemeyer_AAVS1_Ranking-RVDs	Primer pair generated using optimal parameters. 
H1
011-068107851F
TCTGAGTGGATGGTGTGTGCTTTG
Hockemeyer_AAVS1_Ranking-RVDs	Primer pair generated using optimal parameters. 
H2
011-068107851R
AGAGCAGACGGCAGACTCCA
Hockemeyer_AAVS1_Ranking-RVDs	Primer pair generated using optimal parameters. 
A3
019-022374062F
AGGTGGCAAACTAGAGGACCC
Hockemeyer_AAVS1_Ranking-RVDs	Primer pair generated using optimal parameters. 
A4
019-022374062R
ACTGGCCATGAACTGTGGCTTAG
Hockemeyer_AAVS1_Ranking-RVDs	Primer pair generated using optimal parameters. 
B3
004-015022872F
CCTATTTTCTGGTGACTATCTTGATTCTTGGC
Hockemeyer_AAVS1_Ranking-RVDs	Warning! Primer pair was generated using suboptimal parameters. 
B4
004-015022872R
CAGATCTTCCCCCAAACCGTTAC
Hockemeyer_AAVS1_Ranking-RVDs	Warning! Primer pair was generated using suboptimal parameters. 
C3
018-070204440F
CCACTCAGGAACACCAGGTTCT
Hockemeyer_AAVS1_Ranking-RVDs	Primer pair generated using optimal parameters. 
C4
018-070204440R
CATTGGGAACCTGTCAAGAAGTGC
Hockemeyer_AAVS1_Ranking-RVDs	Primer pair generated using optimal parameters. 
D3
012-030180063F
CTATAAGGCTGCTGGGCCTTTC
Hockemeyer_AAVS1_Ranking-RVDs	Primer pair generated using optimal parameters. 
D4
012-030180063R
GAGGCAAACCTACTTTATGGAGGG
Hockemeyer_AAVS1_Ranking-RVDs	Primer pair generated using optimal parameters. 
E3
008-145493530F
GAGCCATTAAGGGCTAGAGGG
Hockemeyer_AAVS1_Ranking-RVDs	Primer pair generated using optimal parameters. 
E4
008-145493530R
GGAAAGCTCCCAAGAAGGGAAG
Hockemeyer_AAVS1_Ranking-RVDs	Primer pair generated using optimal parameters. 
F3
008-145324445F
GAGCCATTAAGGGCTAGAGGG
Hockemeyer_AAVS1_Ranking-RVDs	Primer pair generated using optimal parameters. 
F4
008-145324445R
GGAAAGCTCCCAAGAAGGGAAG
Hockemeyer_AAVS1_Ranking-RVDs	Primer pair generated using optimal parameters. 
G3
015-074823089F
CACCTGTGTAGGTATCTGTGGAC
Hockemeyer_AAVS1_Ranking-RVDs	Primer pair generated using optimal parameters. 
G4
015-074823089R
CTGTCAGAGCAAAAGGGAGCAGG
Hockemeyer_AAVS1_Ranking-RVDs	Primer pair generated using optimal parameters. 
H3
00X-074326678F
CCCTTCCCTTCAAGGACACC
Hockemeyer_AAVS1_Ranking-RVDs	Primer pair generated using optimal parameters. 
H4
00X-074326678R
GCTGAGTTTCGTCCTGTTTCTGG
Hockemeyer_AAVS1_Ranking-RVDs	Primer pair generated using optimal parameters. 
A5
00X-085476489F
GGAAATGTGAGGCCATCAGCTTTG
Hockemeyer_AAVS1_Ranking-RVDs	Primer pair generated using optimal parameters. 
A6
00X-085476489R
CAGCCCCCAGAACTGTGAGA
Hockemeyer_AAVS1_Ranking-RVDs	Primer pair generated using optimal parameters. 
B5
001-114585589F
CCCCATAAATGATCTGCATCCAGG
Hockemeyer_AAVS1_Ranking-RVDs	Primer pair generated using optimal parameters. 
B6
001-114585589R
CGCTGAGAAGGCCAGATGAG
Hockemeyer_AAVS1_Ranking-RVDs	Primer pair generated using optimal parameters. 
C5
020-058618528F
GGGAGCTATGATCATGCCATAGG
Hockemeyer_AAVS1_Ranking-RVDs	Primer pair generated using optimal parameters. 
C6
020-058618528R
GCTCCATGTCATTGTTCTGGGC
Hockemeyer_AAVS1_Ranking-RVDs	Primer pair generated using optimal parameters. 
D5
001-062633493F
TCCTCCTGCCTAATGGCTTTACTG
Hockemeyer_AAVS1_Ranking-RVDs	Primer pair generated using optimal parameters. 
D6
001-062633493R
GCTACTGAGCCCAGCCTAATTTAC
Hockemeyer_AAVS1_Ranking-RVDs	Primer pair generated using optimal parameters. 
E5
019-019017199F
GGGATGGGAGCAGATCCACA
Hockemeyer_AAVS1_Ranking-RVDs	Primer pair generated using optimal parameters. 
E6
019-019017199R
AAGCCTTCTGTGTCTCGGCC
Hockemeyer_AAVS1_Ranking-RVDs	Primer pair generated using optimal parameters. 
F5
007-031005868F
GTCAAAGTCTCTACTCTCTGACCC
Hockemeyer_AAVS1_Ranking-RVDs	Primer pair generated using optimal parameters. 
F6
007-031005868R
GGGAGAAGAGCATCTGGAACAGAA
Hockemeyer_AAVS1_Ranking-RVDs	Primer pair generated using optimal parameters. 
G5
002-064716657F
GAAATGCATCCCCTCTAGCCTG
Hockemeyer_AAVS1_Ranking-RVDs	Primer pair generated using optimal parameters. 
G6
002-064716657R
GCTGCATGGACAGTGCTCACA
Hockemeyer_AAVS1_Ranking-RVDs	Primer pair generated using optimal parameters. 
H5
020-050472318F
CTGGGTCATGTGGTACTGCATGT
Hockemeyer_AAVS1_Ranking-RVDs	Primer pair generated using optimal parameters. 
H6
020-050472318R
CTGCAGTTGATCCATCCAGACC
Hockemeyer_AAVS1_Ranking-RVDs	Primer pair generated using optimal parameters. 
A7
005-172898571F
GCGACAAGGAAATGGAGTCATGC
Hockemeyer_AAVS1_Ranking-RVDs	Primer pair generated using optimal parameters. 
A8
005-172898571R
GGCCTGTGTTTCAGAGTTGACTTC
Hockemeyer_AAVS1_Ranking-RVDs	Primer pair generated using optimal parameters. 
B7
022-037091993F
CTGCGAACTAACCTGGAAACGTAC
Hockemeyer_AAVS1_Ranking-RVDs	Primer pair generated using optimal parameters. 
B8
022-037091993R
GAGAGGAAGAGAGACTGAACGC
Hockemeyer_AAVS1_Ranking-RVDs	Primer pair generated using optimal parameters. 
C7
00X-040907277F
CTGGTTAGGAGGGTTGAAGGTG
Hockemeyer_AAVS1_Ranking-RVDs	Primer pair generated using optimal parameters. 
C8
00X-040907277R
GGTCAGAGAAGGCTGAGTAGG
Hockemeyer_AAVS1_Ranking-RVDs	Primer pair generated using optimal parameters. 
D7
013-112646719F
CTTGGCCTCTCTGAACACCG
Hockemeyer_AAVS1_Ranking-RVDs	Primer pair generated using optimal parameters. 
D8
013-112646719R
CATCCTGGTCCTGCACTTTTAGC
Hockemeyer_AAVS1_Ranking-RVDs	Primer pair generated using optimal parameters. 
E7
020-031067101F
CTGCTCAGAGACCCAAGGTG
Hockemeyer_AAVS1_Ranking-RVDs	Primer pair generated using optimal parameters. 
E8
020-031067101R
TAGAGACAGCGTCTCACTGTGTTG
Hockemeyer_AAVS1_Ranking-RVDs	Primer pair generated using optimal parameters. 
F7
018-013545631F
GCAGTGCGGTTTCTTAGCTGCAA
Hockemeyer_AAVS1_Ranking-RVDs	Primer pair generated using optimal parameters. 
F8
018-013545631R
CAGCTCTGTGACTATGACTTCCC
Hockemeyer_AAVS1_Ranking-RVDs	Primer pair generated using optimal parameters. 
G7
021-036773953F
CCAGAGCGGTTTTCCAAGATGC
Hockemeyer_AAVS1_Ranking-RVDs	Primer pair generated using optimal parameters. 
G8
021-036773953R
GGATTCACAACACCATGGATGGAC
Hockemeyer_AAVS1_Ranking-RVDs	Primer pair generated using optimal parameters. 
H7
002-068331837F
GAGGAGAGGTGTCAGGCTCTT
Hockemeyer_AAVS1_Ranking-RVDs	Primer pair generated using optimal parameters. 
H8
002-068331837R
GATGAGTAGAGGCACATAGGTGAC
Hockemeyer_AAVS1_Ranking-RVDs	Primer pair generated using optimal parameters. 
A9
007-127776243F
ATGACAGAAGGGGCGTGGTG
Hockemeyer_AAVS1_Ranking-RVDs	Primer pair generated using optimal parameters. 
A10
007-127776243R
AGTGGCGCCTCCTAGAAGCA
Hockemeyer_AAVS1_Ranking-RVDs	Primer pair generated using optimal parameters. 
B9
004-009669000F
ACTGAGGGAACTCAGAGGCC
Hockemeyer_AAVS1_Ranking-RVDs	Primer pair generated using optimal parameters. 
B10
004-009669000R
TGGGGATAAAGGAGGGGAGG
Hockemeyer_AAVS1_Ranking-RVDs	Primer pair generated using optimal parameters. 
C9
010-050821729F
GTGGGGGATGCTTTTGTCTGAG
Hockemeyer_AAVS1_Ranking-RVDs	Primer pair generated using optimal parameters. 
C10
010-050821729R
AAGTCTCTGCTCGCTGCCCT
Hockemeyer_AAVS1_Ranking-RVDs	Primer pair generated using optimal parameters. 
D9
002-240313358F
CCAGAGCCATGGAACACAGG
Hockemeyer_AAVS1_Ranking-RVDs	Primer pair generated using optimal parameters. 
D10
002-240313358R
AGCGTGCCAGCTTCAGCACA
Hockemeyer_AAVS1_Ranking-RVDs	Primer pair generated using optimal parameters. 
E9
012-025903897F
CCAGCATCTTGTGGCTGAGTAG
Hockemeyer_AAVS1_Ranking-RVDs	Primer pair generated using optimal parameters. 
E10
012-025903897R
GGGAAGATGACAGTGTTCTTGACAGG
Hockemeyer_AAVS1_Ranking-RVDs	Primer pair generated using optimal parameters. 
F9
005-157580667F
GTAATCCAGGGTCTCTGTTTGTCC
Hockemeyer_AAVS1_Ranking-RVDs	Primer pair generated using optimal parameters. 
F10
005-157580667R
GTCTTTGGGTGGCATTTGTGAGG
Hockemeyer_AAVS1_Ranking-RVDs	Primer pair generated using optimal parameters. 
G9
003-048475638F
GTGGCCTCCAGTTTATAAACCCAG
Hockemeyer_AAVS1_Ranking-RVDs	Primer pair generated using optimal parameters. 
G10
003-048475638R
GCTGCGGTACTGTTACTGTGTG
Hockemeyer_AAVS1_Ranking-RVDs	Primer pair generated using optimal parameters. 
H9
007-030310150F
CTGCTGCCAAAAGCTACCCATG
Hockemeyer_AAVS1_Ranking-RVDs	Primer pair generated using optimal parameters. 
H10
007-030310150R
GAGCTCCAGTTTCCTGCACTGT
Hockemeyer_AAVS1_Ranking-RVDs	Primer pair generated using optimal parameters. 
A11
007-123033818F
CTACCCCTATGCAGGTTTGCTGA
Hockemeyer_AAVS1_Ranking-RVDs	Primer pair generated using optimal parameters. 
A12
007-123033818R
CTTCCCCCAACTCTCACAGG
Hockemeyer_AAVS1_Ranking-RVDs	Primer pair generated using optimal parameters. 
B11
006-157508829F
GCCAGCCTCCTCTATTAATGGAGT
Hockemeyer_AAVS1_Ranking-RVDs	Primer pair generated using optimal parameters. 
B12
006-157508829R
GAGTAAAGGAAGAAAGCCGTCTTTTGCAG
Hockemeyer_AAVS1_Ranking-RVDs	Primer pair generated using optimal parameters. 
C11
012-120471484F
GCCGGCTGAGAGTTCTTGCT
Hockemeyer_AAVS1_Ranking-RVDs	Primer pair generated using optimal parameters. 
C12
012-120471484R
GTATGCCTGTATCTGGCTGTGTTG
Hockemeyer_AAVS1_Ranking-RVDs	Primer pair generated using optimal parameters. 
D11
015-066339107F
CACACAGTAGGCTTTGCTGGC
Hockemeyer_AAVS1_Ranking-RVDs	Primer pair generated using optimal parameters. 
D12
015-066339107R
GGTGAACTTCATGGGATGTGCTG
Hockemeyer_AAVS1_Ranking-RVDs	Primer pair generated using optimal parameters. 
E11
017-008242168F
CAGCTATTGCGGGGAGCTAATG
Hockemeyer_AAVS1_Ranking-RVDs	Primer pair generated using optimal parameters. 
E12
017-008242168R
AGGCCACAGCTCAGAACTGTGGA
Hockemeyer_AAVS1_Ranking-RVDs	Primer pair generated using optimal parameters. 
F11
015-067305574F
CCCCACCTTGCTGAATGGAC
Hockemeyer_AAVS1_Ranking-RVDs	Primer pair generated using optimal parameters. 
F12
015-067305574R
CCAGTCCTTGATCTCTTCCTGAGT
Hockemeyer_AAVS1_Ranking-RVDs	Primer pair generated using optimal parameters. 
G11
010-035501885F
CAGGATGCTGTGTCCCCTCA
Hockemeyer_AAVS1_Ranking-RVDs	Primer pair generated using optimal parameters. 
G12
010-035501885R
GAGCTCCGACTCTGAAGGAAG
Hockemeyer_AAVS1_Ranking-RVDs	Primer pair generated using optimal parameters. 
H11
007-140379038F
TCTGCGCCCCTTTGTCACCA
Hockemeyer_AAVS1_Ranking-RVDs	Primer pair generated using optimal parameters. 
H12
007-140379038R
CCTCTAAGGCAGGTCACCAAC
Hockemeyer_AAVS1_Ranking-RVDs	Primer pair generated using optimal parameters. 
Primer Plate #3
A1
006-092651079F
CAGCAAGGAGGCCAAGAACTCA
Hockemeyer_AAVS1_Ranking-RVDs	Primer pair generated using optimal parameters. 
A2
006-092651079R
GTGTGTGGGCCTCAAGTTTTGTAC
Hockemeyer_AAVS1_Ranking-RVDs	Primer pair generated using optimal parameters. 
B1
003-070995805F
GCCGTTGCTTTTGCCTTGAACATC
Hockemeyer_AAVS1_Ranking-RVDs	Primer pair generated using optimal parameters. 
B2
003-070995805R
TACTTGAGTCCCTGGATCTGGC
Hockemeyer_AAVS1_Ranking-RVDs	Primer pair generated using optimal parameters. 
C1
002-001827851F
GGGGGTTGGGGAACGTTTTTAG
Hockemeyer_AAVS1_Ranking-RVDs	Primer pair generated using optimal parameters. 
C2
002-001827851R
TCAGGACGAGAGCAGCTCAAATG
Hockemeyer_AAVS1_Ranking-RVDs	Primer pair generated using optimal parameters. 
D1
010-024751913F
GGTGGATGTGCAGAGATGGAG
Hockemeyer_AAVS1_Ranking-RVDs	Primer pair generated using optimal parameters. 
D2
010-024751913R
CTCTACTGAGCCCTCCAGTG
Hockemeyer_AAVS1_Ranking-RVDs	Primer pair generated using optimal parameters.