PROGNOS Summary for Hockemeyer_AAVS1_Ranking-Homology_5T

The hg19 genome was searched for the TALEN pair targeting:
5'- TTTTCTGTCACCAATCCT NN..NN ACTGTGGGGTGGAGGGGA -3'

Up to 5 mismatches were allowed in each nuclease half-site.

Allowed spacing distances were: 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26

Only heterodimers of the nuclease pair were included in the output

157 total sites were located
Mismatch Types
Genomic Regions
  0 and 0: 1
  2 and 5: 1
  3 and 5: 4
  4 and 4: 1
  4 and 5: 33
  5 and 5: 117
  Exons: 6
  Promoters: 1
  Introns: 70
  Intergenic: 80


The top 100 sites are ranked below according to a weighted sequence homology. All sites listed contain flanking 5' thymidines.
For the full output, click here.

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Primer pairs were designed using the 400 bp of sequence on either side of the cleavage site to melt close to 60 °C

For easy visualization of cleavage on gels:
The smallest fragment will be longer than 130 bp, which is also the minimum separation distance between uncleaved and cleaved products.
The two cleavage products will be separated by a minimum size of 0 bp
To optimize assay conditions, the amplicons have a minimum length of 260 bp, a maximum length of 450 bp, and an optimal length of 270 bp.
Rank: 1	Homology Score: 72
	Genomic Region: Intron	Closest Gene: PPP1R12C	Caution! This site scores very closely to the intended nuclease target. The potential for cleavage at this site is of great concern.
Match Type: L15R
	           Left Nuclease
	         TTTTCTGTCACCAATCCT
	5'-GGGGC TTTTCTGTCACCAATCCT      GTCCCTAGTGGCCCC       ACTGTGGGGTGGAGGGGA CAGAT-3'
	3'-CCCCG AAAAGACAGTGGTTAGGA      CAGGGATCACCGGGG       TGACACCCCACCTCCCCT GTCTA-5'
	                                                       TGACACCCCACCTCCCCT
	                                                            Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 019-055627003
Forward Primer: 5'-CCAGGAAATGGGGGTGTGTC-3' Tm: 59.508
Reverse Primer: 5'-GTCCACTTCAGGACAGCATGTTTG-3' Tm: 59.038
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 131, 139
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr19:55627107-55627157

Rank: 2	Homology Score: 17
	Genomic Region: Intergenic	Closest Gene: TFEC
Match Type: R11L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-CCCCC TCCCCGCCACCCCCCAGT        AATGGGGAGAG         AGGACATTTGACAGAGAA TGAAG-3'
	3'-GGGGG AGGGGCGGTGGGGGGTCA        TTACCCCTCTC         TCCTGTAAACTGTCTCTT ACTTC-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 007-115754830
Forward Primer: 5'-CGGTTGAGACTGAAGCATGCAAAG-3' Tm: 59.038
Reverse Primer: 5'-AAGGACCACACTACTGACATTAGATCTACC-3' Tm: 60.058
Expected PCR Product Length: 274
Expected CelI Cleavage Product Sizes: 143, 131
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr7:115754948-115754994

Rank: 3	Homology Score: 10
	Genomic Region: Intron	Closest Gene: ERCC5
Match Type: L20R
	           Left Nuclease
	         TTTTCTGTCACCAATCCT
	5'-CTTTA TTTTCTTTCTGCAACCAT    GAAGTCTCTGGAAGTGGTGG    ACTGTAGGGTGGTGTGGA GTAGC-3'
	3'-GAAAT AAAAGAAAGACGTTGGTA    CTTCAGAGACCTTCACCACC    TGACATCCCACCACACCT CATCG-5'
	                                                       TGACACCCCACCTCCCCT
	                                                            Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 013-103511881
Forward Primer: 5'-TCTCTGGCTGGCAGTTGAGG-3' Tm: 59.508
Reverse Primer: 5'-CAGGTCACTGAAAACTGCAAACCG-3' Tm: 59.038
Expected PCR Product Length: 346
Expected CelI Cleavage Product Sizes: 131, 215
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr13:103511982-103512037

Rank: 4	Homology Score: 10
	Genomic Region: Intron	Closest Gene: MYT1L
Match Type: R15L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-GAATG TTCCCTCCAACCCAGAGT      GCTGAGAAGGCTGGC       AGGATAGAGGCCAGAACA TGTCT-3'
	3'-CTTAC AAGGGAGGTTGGGTCTCA      CGACTCTTCCGACCG       TCCTATCTCCGGTCTTGT ACAGA-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 002-001827851
Forward Primer: 5'-GGGGGTTGGGGAACGTTTTTAG-3' Tm: 59.258
Reverse Primer: 5'-TCAGGACGAGAGCAGCTCAAATG-3' Tm: 59.148
Expected PCR Product Length: 446
Expected CelI Cleavage Product Sizes: 315, 131
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr2:1828139-1828189

Rank: 5	Homology Score: 10
	Genomic Region: Intron	Closest Gene: STPG1
Match Type: R11L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-ACACT TCCCCACCACACCACAAT        GCCCTGTCTCA         AGAAACTGTGACAGAATA AAATG-3'
	3'-TGTGA AGGGGTGGTGTGGTGTTA        CGGGACAGAGT         TCTTTGACACTGTCTTAT TTTAC-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 001-024697363
Forward Primer: 5'-AGCCTCCCAAAGTGCTAGGAC-3' Tm: 59.378
Reverse Primer: 5'-GTGGAGAACATCCATTCACAGGG-3' Tm: 59.148
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 135, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr1:24697473-24697519

Rank: 6	Homology Score: 10
	Genomic Region: Intergenic	Closest Gene: FOXP1
Match Type: R20L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-TGAAA TGCCCCCCACCCCAGAGT    ATATTCATTAGGACTCTTTG    ATTATAAGAGACAGAAAA TCAAC-3'
	3'-ACTTT ACGGGGGGTGGGGTCTCA    TATAAGTAATCCTGAGAAAC    TAATATTCTCTGTCTTTT AGTTG-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 003-070995805
Forward Primer: 5'-GCCGTTGCTTTTGCCTTGAACATC-3' Tm: 59.038
Reverse Primer: 5'-TACTTGAGTCCCTGGATCTGGC-3' Tm: 59.258
Expected PCR Product Length: 306
Expected CelI Cleavage Product Sizes: 176, 130
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr3:70995951-70996006

Rank: 7	Homology Score: 8
	Genomic Region: Intergenic	Closest Gene: INADL
Match Type: R25L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-TCAAC TCCTCTCAACCCCAGAGC CATGTGTTACTCTTTGGGGAACTTC  AGGATAGCTGTCTGAAAA CAGTC-3'
	3'-AGTTG AGGAGAGTTGGGGTCTCG GTACACAATGAGAAACCCCTTGAAG  TCCTATCGACAGACTTTT GTCAG-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 001-062633493
Forward Primer: 5'-TCCTCCTGCCTAATGGCTTTACTG-3' Tm: 59.038
Reverse Primer: 5'-GCTACTGAGCCCAGCCTAATTTAC-3' Tm: 59.038
Expected PCR Product Length: 273
Expected CelI Cleavage Product Sizes: 130, 143
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr1:62633591-62633651

Rank: 8	Homology Score: 5
	Genomic Region: Exon	Closest Gene: CDKAL1
Match Type: R16L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-TTCCC TCCCCTTCTCTCCACAGA      TTGGTGAAAGACAACA      AGTGTTAGTAACAGAAGA ATCTT-3'
	3'-AAGGG AGGGGAAGAGAGGTGTCT      AACCACTTTCTGTTGT      TCACAATCATTGTCTTCT TAGAA-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 006-021198081
Forward Primer: 5'-CCGTGATTTGAACAGGGTTCGC-3' Tm: 59.258
Reverse Primer: 5'-CTCTCCCCTTTAAATGCCCTTTGC-3' Tm: 59.038
Expected PCR Product Length: 341
Expected CelI Cleavage Product Sizes: 182, 159
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr6:21198235-21198286

Rank: 9	Homology Score: 5
	Genomic Region: Exon	Closest Gene: IL17F
Match Type: L18R
	           Left Nuclease
	         TTTTCTGTCACCAATCCT
	5'-GAATA TTTTCTGTTTCCATCCGT     GCAGGTCTTATTAAGAGT     CCTGTGAAGTGGAGGGAA TTGGG-3'
	3'-CTTAT AAAAGACAAAGGTAGGCA     CGTCCAGAATAATTCTCA     GGACACTTCACCTCCCTT AACCC-5'
	                                                       TGACACCCCACCTCCCCT
	                                                            Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 006-052101450
Forward Primer: 5'-GCTGGGAATGCAAACAAACACCTG-3' Tm: 59.038
Reverse Primer: 5'-TCATCCACCATGTGCAGTAAGAGG-3' Tm: 59.038
Expected PCR Product Length: 299
Expected CelI Cleavage Product Sizes: 169, 130
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr6:52101590-52101643

Rank: 10	Homology Score: 5
	Genomic Region: Intron	Closest Gene: PVRL1
Match Type: R17L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-TAAAG TCCTCTGCCACCCACACT     CTTCATGAGATCCCAAA      AGGATTGATCATGGAAAA TGCAA-3'
	3'-ATTTC AGGAGACGGTGGGTGTGA     GAAGTACTCTAGGGTTT      TCCTAACTAGTACCTTTT ACGTT-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 011-119574363
Forward Primer: 5'-CAACGAGGCTGACGCTCTTTC-3' Tm: 59.378
Reverse Primer: 5'-CTGAGCACCTGTGTGCCAGT-3' Tm: 59.508
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 135, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr11:119574470-119574522

Rank: 11	Homology Score: 5
	Genomic Region: Intron	Closest Gene: HEG1
Match Type: R18L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-CTCTA TCCCTTCCACCTCACCAA     ATAGTCCTCGCTAGCAGA     AGGGTTGGGTACAGAACA ACAGT-3'
	3'-GAGAT AGGGAAGGTGGAGTGGTT     TATCAGGAGCGATCGTCT     TCCCAACCCATGTCTTGT TGTCA-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 003-124702567
Forward Primer: 5'-TGCAAGTCTACAACCAGGGTGTG-3' Tm: 59.148
Reverse Primer: 5'-CAGAAGGCAGCTGGAAGTCTC-3' Tm: 59.378
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 134, 136
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr3:124702672-124702725

Rank: 12	Homology Score: 5
	Genomic Region: Intron	Closest Gene: PPP2R3A
Match Type: L21R
	           Left Nuclease
	         TTTTCTGTCACCAATCCT
	5'-CCTCG TTCTCTGTCTCCCTTCCC   TTTCAAACTCTGCTTCTCAGC    ACTTTGGGGTAAAAGGGA GGGGT-3'
	3'-GGAGC AAGAGACAGAGGGAAGGG   AAAGTTTGAGACGAAGAGTCG    TGAAACCCCATTTTCCCT CCCCA-5'
	                                                       TGACACCCCACCTCCCCT
	                                                            Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 003-135693383
Forward Primer: 5'-CTGTGCTTGCTTTGTCCCAGTC-3' Tm: 59.258
Reverse Primer: 5'-CCTTGCTAGGATTCCTAAGCCC-3' Tm: 59.258
Expected PCR Product Length: 377
Expected CelI Cleavage Product Sizes: 139, 238
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr3:135693492-135693548

Rank: 13	Homology Score: 5
	Genomic Region: Intron	Closest Gene: KCNT1
Match Type: L14R
	           Left Nuclease
	         TTTTCTGTCACCAATCCT
	5'-GTGGC TTCCCCGTCACCACTGCT       GTGGCCCACTGCCC       ACTGAGCAGAGGAGGGGA CGGGG-3'
	3'-CACCG AAGGGGCAGTGGTGACGA       CACCGGGTGACGGG       TGACTCGTCTCCTCCCCT GCCCC-5'
	                                                       TGACACCCCACCTCCCCT
	                                                            Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 009-138652006
Forward Primer: 5'-GGGAAGGGTCAAGGTGGACA-3' Tm: 59.508
Reverse Primer: 5'-AATAGCAGGGTCCCTCCAGGGT-3' Tm: 61.288
Expected PCR Product Length: 287
Expected CelI Cleavage Product Sizes: 131, 156
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr9:138652110-138652159

Rank: 14	Homology Score: 5
	Genomic Region: Intron	Closest Gene: BOP1
Match Type: L22R
	           Left Nuclease
	         TTTTCTGTCACCAATCCT
	5'-CCAAA TTTCCTCTCCCTAATCCT   GCAGGAGCCCACAGCTGGCCAG   AGGGAGGGGAGGAGAGGA CAAGC-3'
	3'-GGTTT AAAGGAGAGGGATTAGGA   CGTCCTCGGGTGTCGACCGGTC   TCCCTCCCCTCCTCTCCT GTTCG-5'
	                                                       TGACACCCCACCTCCCCT
	                                                            Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 008-145493530
Forward Primer: 5'-GAGCCATTAAGGGCTAGAGGG-3' Tm: 59.378
Reverse Primer: 5'-GGAAAGCTCCCAAGAAGGGAAG-3' Tm: 59.258
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 135, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr8:145493634-145493691

Rank: 15	Homology Score: 5
	Genomic Region: Intron	Closest Gene: ARHGAP10
Match Type: R17L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-TCCTG TTCCCACCACCCCACAAC     CTTCTTACATCTGAACA      ATGACAGATGACAGTAAA TCCAG-3'
	3'-AGGAC AAGGGTGGTGGGGTGTTG     GAAGAATGTAGACTTGT      TACTGTCTACTGTCATTT AGGTC-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 004-148942680
Forward Primer: 5'-GTAGGTAAGCCAGGGAGCTGT-3' Tm: 59.378
Reverse Primer: 5'-CCTGCCAACACCATGTTTCCAC-3' Tm: 59.258
Expected PCR Product Length: 299
Expected CelI Cleavage Product Sizes: 158, 141
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr4:148942810-148942862

Rank: 16	Homology Score: 5
	Genomic Region: Intron	Closest Gene: PTPRN2
Match Type: L25R
	           Left Nuclease
	         TTTTCTGTCACCAATCCT
	5'-AGAGA TCTGCTGTGACCACACCT GCAGGGTACTCAGGCCACCTTTCAT  ACTGGGGTGAGGAGAGGA AGTAA-3'
	3'-TCTCT AGACGACACTGGTGTGGA CGTCCCATGAGTCCGGTGGAAAGTA  TGACCCCACTCCTCTCCT TCATT-5'
	                                                       TGACACCCCACCTCCCCT
	                                                            Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 007-158192599
Forward Primer: 5'-TAGTCCCCAGGCACTTGCAG-3' Tm: 59.508
Reverse Primer: 5'-GCCTCGTAAGTTTAGGCATCCTC-3' Tm: 59.148
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 137, 133
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr7:158192704-158192764

Rank: 17	Homology Score: 5
	Genomic Region: Intron	Closest Gene: KIAA1217
Match Type: L20R
	           Left Nuclease
	         TTTTCTGTCACCAATCCT
	5'-GACAT TTTTCTTTTATCCATCCT    ACAGCACTGCACAATTATTG    ATTGTGGCTTGGAAGCGA AATGA-3'
	3'-CTGTA AAAAGAAAATAGGTAGGA    TGTCGTGACGTGTTAATAAC    TAACACCGAACCTTCGCT TTACT-5'
	                                                       TGACACCCCACCTCCCCT
	                                                            Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 010-024751913
Forward Primer: 5'-GGTGGATGTGCAGAGATGGAG-3' Tm: 59.378
Reverse Primer: 5'-CTCTACTGAGCCCTCCAGTG-3' Tm: 59.508
Expected PCR Product Length: 343
Expected CelI Cleavage Product Sizes: 208, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr10:24752091-24752146

Rank: 18	Homology Score: 5
	Genomic Region: Intron	Closest Gene: DMD
Match Type: L23R
	           Left Nuclease
	         TTTTCTGTCACCAATCCT
	5'-TCTTC TCTTCCATCACTAATTCT  GATGAACAGCTATCAAGCTATTC   ATGGTAGGGTGGAGGGTA GGTGG-3'
	3'-AGAAG AGAAGGTAGTGATTAAGA  CTACTTGTCGATAGTTCGATAAG   TACCATCCCACCTCCCAT CCACC-5'
	                                                       TGACACCCCACCTCCCCT
	                                                            Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 00X-031479613
Forward Primer: 5'-GTCTGGACATGTCTGAATGCCTAG-3' Tm: 59.038
Reverse Primer: 5'-GGTTAGGTAAAGTGTCCCCTGC-3' Tm: 59.258
Expected PCR Product Length: 339
Expected CelI Cleavage Product Sizes: 156, 183
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chrX:31479738-31479796

Rank: 19	Homology Score: 5
	Genomic Region: Intron	Closest Gene: ZNF25
Match Type: L15R
	           Left Nuclease
	         TTTTCTGTCACCAATCCT
	5'-AAAAA TTTTTTGTCCCATTTCCT      GTCTTTAGAAACTAG       CCTCTGGGCTGGATGGGA TTCCC-3'
	3'-TTTTT AAAAAACAGGGTAAAGGA      CAGAAATCTTTGATC       GGAGACCCGACCTACCCT AAGGG-5'
	                                                       TGACACCCCACCTCCCCT
	                                                            Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 010-038264363
Forward Primer: 5'-GGTGCTTACGAAATGCCTAAGCTC-3' Tm: 59.038
Reverse Primer: 5'-AGGCCTTCCTGCATCCTCCA-3' Tm: 59.508
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 135, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr10:38264471-38264521

Rank: 20	Homology Score: 5
	Genomic Region: Intron	Closest Gene: NFATC2
Match Type: L25R
	           Left Nuclease
	         TTTTCTGTCACCAATCCT
	5'-TCGGG TTTGCTGTCACAACAACT GCAGGATGACCCGGTGCTGGGCAGC  ACTGCTGGCTGGAGGTGA GAAAT-3'
	3'-AGCCC AAACGACAGTGTTGTTGA CGTCCTACTGGGCCACGACCCGTCG  TGACGACCGACCTCCACT CTTTA-5'
	                                                       TGACACCCCACCTCCCCT
	                                                            Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 020-050029460
Forward Primer: 5'-AGAAGCACAGGATGAGGCCG-3' Tm: 59.508
Reverse Primer: 5'-CAGTGGATTGACAGAGGCAGATG-3' Tm: 59.148
Expected PCR Product Length: 336
Expected CelI Cleavage Product Sizes: 205, 131
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr20:50029633-50029693

Rank: 21	Homology Score: 5
	Genomic Region: Intron	Closest Gene: ERC2
Match Type: L11R
	           Left Nuclease
	         TTTTCTGTCACCAATCCT
	5'-TTCAC TTTTCTGGGACAAATGCT        ACCAGAGTGCA         ACTGCTGGGTTGAATGGA AGTTG-3'
	3'-AAGTG AAAAGACCCTGTTTACGA        TGGTCTCACGT         TGACGACCCAACTTACCT TCAAC-5'
	                                                       TGACACCCCACCTCCCCT
	                                                            Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 003-056061208
Forward Primer: 5'-GGCATGGATATCCAGGCTTGCT-3' Tm: 59.258
Reverse Primer: 5'-TGGGGATGTACAGAGGCCAG-3' Tm: 59.508
Expected PCR Product Length: 292
Expected CelI Cleavage Product Sizes: 161, 131
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr3:56061344-56061390

Rank: 22	Homology Score: 5
	Genomic Region: Intron	Closest Gene: AFF1
Match Type: L21R
	           Left Nuclease
	         TTTTCTGTCACCAATCCT
	5'-TTCTC TATTCTGCCTCAAAACCT   AGTTGCGGTTGTTCCTTGAGC    ACTGTGGCATGGAGCTGA GTTTC-3'
	3'-AAGAG ATAAGACGGAGTTTTGGA   TCAACGCCAACAAGGAACTCG    TGACACCGTACCTCGACT CAAAG-5'
	                                                       TGACACCCCACCTCCCCT
	                                                            Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 004-088055877
Forward Primer: 5'-TGGGGTGTTTGGATTGGGAGG-3' Tm: 59.378
Reverse Primer: 5'-TCAGCTAGCATTTCAGCTCAGGC-3' Tm: 59.148
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 135, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr4:88055982-88056038

Rank: 23	Homology Score: 5
	Genomic Region: Intron	Closest Gene: LOC100188947
Match Type: R17L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-TCTTC TGCCTGCCACCCCTCAGT     GGAAGCCTGGCAGAGAG      TGGATGGCTGTCAGAAGA TGCTG-3'
	3'-AGAAG ACGGACGGTGGGGAGTCA     CCTTCGGACCGTCTCTC      ACCTACCGACAGTCTTCT ACGAC-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 010-093286041
Forward Primer: 5'-GATTCTGAGCAGGGAGCTTGG-3' Tm: 59.378
Reverse Primer: 5'-ACAGGATGAGCTAGGGCCTGA-3' Tm: 59.378
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 135, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr10:93286148-93286200

Rank: 24	Homology Score: 5
	Genomic Region: Intron	Closest Gene: TBC1D12
Match Type: L19R
	           Left Nuclease
	         TTTTCTGTCACCAATCCT
	5'-CTTTC TTTTCTGTAGCAAATTCT    AGATCTATTTTTTCTTATT     TCTTTGAGGTGGGGAGGA GTAGT-3'
	3'-GAAAG AAAAGACATCGTTTAAGA    TCTAGATAAAAAAGAATAA     AGAAACTCCACCCCTCCT CATCA-5'
	                                                       TGACACCCCACCTCCCCT
	                                                            Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 010-096170810
Forward Primer: 5'-GGCATGCCAGAGATAGGACTATTC-3' Tm: 59.038
Reverse Primer: 5'-GTGCTACAGTGGCAACCTTCATG-3' Tm: 59.148
Expected PCR Product Length: 330
Expected CelI Cleavage Product Sizes: 191, 139
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr10:96170972-96171026

Rank: 25	Homology Score: 5
	Genomic Region: Intron	Closest Gene: STARD7
Match Type: R17L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-CTCCG TGCCCTCCACCTCACCCT     ACCCCCTGCAACATTTA      AAGAAAGGTTACAGATAA GAAAC-3'
	3'-GAGGC ACGGGAGGTGGAGTGGGA     TGGGGGACGTTGTAAAT      TTCTTTCCAATGTCTATT CTTTG-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 002-096866458
Forward Primer: 5'-CGCCGAAACTGGCCTCAAATTCC-3' Tm: 61.088
Reverse Primer: 5'-CTGCCTAGCAGTTACCTTTGTGG-3' Tm: 59.148
Expected PCR Product Length: 295
Expected CelI Cleavage Product Sizes: 130, 165
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr2:96866560-96866612

Rank: 26	Homology Score: 5
	Genomic Region: Intergenic	Closest Gene: LOC729013
Match Type: L25R
	           Left Nuclease
	         TTTTCTGTCACCAATCCT
	5'-AGGTC TCCTCTGACACCACTCCA GTGAGTAGTGGAAAGATGCCTTATT  ACTGCTGGGTGGGGGTGA AGGTC-3'
	3'-TCCAG AGGAGACTGTGGTGAGGT CACTCATCACCTTTCTACGGAATAA  TGACGACCCACCCCCACT TCCAG-5'
	                                                       TGACACCCCACCTCCCCT
	                                                            Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 011-011075984
Forward Primer: 5'-GCCTCATTACTGCTAGGTGGAAG-3' Tm: 59.148
Reverse Primer: 5'-CACCCTAACATCCAGGCATGATGA-3' Tm: 59.038
Expected PCR Product Length: 316
Expected CelI Cleavage Product Sizes: 152, 164
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr11:11076104-11076164

Rank: 27	Homology Score: 5
	Genomic Region: Intergenic	Closest Gene: SCXB
Match Type: L22R
	           Left Nuclease
	         TTTTCTGTCACCAATCCT
	5'-CCAAA TTTCCTCTCCCTAATCCT   GCAGGAGCCCACAGCTGGCCAG   AGGGAGGGGAGGAGAGGA CAAGC-3'
	3'-GGTTT AAAGGAGAGGGATTAGGA   CGTCCTCGGGTGTCGACCGGTC   TCCCTCCCCTCCTCTCCT GTTCG-5'
	                                                       TGACACCCCACCTCCCCT
	                                                            Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 008-145324445
Forward Primer: 5'-GAGCCATTAAGGGCTAGAGGG-3' Tm: 59.378
Reverse Primer: 5'-GGAAAGCTCCCAAGAAGGGAAG-3' Tm: 59.258
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 135, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr8:145324549-145324606

Rank: 28	Homology Score: 5
	Genomic Region: Intergenic	Closest Gene: CLINT1
Match Type: L15R
	           Left Nuclease
	         TTTTCTGTCACCAATCCT
	5'-TGCAC TTTTCAGCCCCCACTCCT      CCTTTATGAAGAGAA       CCTGTGTGCTGGTGGGAA GAGCT-3'
	3'-ACGTG AAAAGTCGGGGGTGAGGA      GGAAATACTTCTCTT       GGACACACGACCACCCTT CTCGA-5'
	                                                       TGACACCCCACCTCCCCT
	                                                            Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 005-157580667
Forward Primer: 5'-GTAATCCAGGGTCTCTGTTTGTCC-3' Tm: 59.038
Reverse Primer: 5'-GTCTTTGGGTGGCATTTGTGAGG-3' Tm: 59.148
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 133, 137
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr5:157580773-157580823

Rank: 29	Homology Score: 5
	Genomic Region: Intergenic	Closest Gene: NUF2
Match Type: R11L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-TTGCT TGACCTCCACACCACACT        CCATCTCTCTT         GGGATAAGTGCCAAAAAA GAGGT-3'
	3'-AACGA ACTGGAGGTGTGGTGTGA        GGTAGAGAGAA         CCCTATTCACGGTTTTTT CTCCA-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 001-163803301
Forward Primer: 5'-GGTTAAGTGCTTGACCACTGCTG-3' Tm: 59.148
Reverse Primer: 5'-CACCATGGCACATGTCTACTTGAG-3' Tm: 59.038
Expected PCR Product Length: 336
Expected CelI Cleavage Product Sizes: 139, 197
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr1:163803415-163803461

Rank: 30	Homology Score: 5
	Genomic Region: Intergenic	Closest Gene: NEK7
Match Type: L25R
	           Left Nuclease
	         TTTTCTGTCACCAATCCT
	5'-GCTGA TTTCCTGACACCAAGGCT CCTCAGCTGAGGCTCCCACCTGCAA  GCTGTGAGCTGGAGATGA ATATC-3'
	3'-CGACT AAAGGACTGTGGTTCCGA GGAGTCGACTCCGAGGGTGGACGTT  CGACACTCGACCTCTACT TATAG-5'
	                                                       TGACACCCCACCTCCCCT
	                                                            Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 001-198360906
Forward Primer: 5'-GGTTTTATAGCCCTTCTCCTGCC-3' Tm: 59.148
Reverse Primer: 5'-GTTGTGGGATTTGAGCTGGTGG-3' Tm: 59.258
Expected PCR Product Length: 300
Expected CelI Cleavage Product Sizes: 130, 170
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr1:198361004-198361064

Rank: 31	Homology Score: 5
	Genomic Region: Intergenic	Closest Gene: ADAM28
Match Type: R12L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-TAATT TACCCTCTACTTGACAGT        GACCACCCACTA        AGTAATGAAGACAGAAAA ATCAG-3'
	3'-ATTAA ATGGGAGATGAACTGTCA        CTGGTGGGTGAT        TCATTACTTCTGTCTTTT TAGTC-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 008-024031178
Forward Primer: 5'-AGTGAGGAGGGGCCTGAAGT-3' Tm: 59.508
Reverse Primer: 5'-CATTTGTTTCGCAGGCTAGCTCC-3' Tm: 59.148
Expected PCR Product Length: 273
Expected CelI Cleavage Product Sizes: 135, 138
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr8:24031287-24031334

Rank: 32	Homology Score: 5
	Genomic Region: Intergenic	Closest Gene: TMTC1
Match Type: L12R
	           Left Nuclease
	         TTTTCTGTCACCAATCCT
	5'-GATTA TTTTCTAACAACCTTCCT        ACTGAGGAACAT        ACTCTGGGATGGATTGGA AGTGC-3'
	3'-CTAAT AAAAGATTGTTGGAAGGA        TGACTCCTTGTA        TGAGACCCTACCTAACCT TCACG-5'
	                                                       TGACACCCCACCTCCCCT
	                                                            Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 012-030180063
Forward Primer: 5'-CTATAAGGCTGCTGGGCCTTTC-3' Tm: 59.258
Reverse Primer: 5'-GAGGCAAACCTACTTTATGGAGGG-3' Tm: 59.038
Expected PCR Product Length: 333
Expected CelI Cleavage Product Sizes: 131, 202
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr12:30180168-30180215

Rank: 33	Homology Score: 5
	Genomic Region: Intergenic	Closest Gene: TSPAN9
Match Type: L12R
	           Left Nuclease
	         TTTTCTGTCACCAATCCT
	5'-TTGGA TTTTCTGACACAAACCCA        TCAGGATCTGGA        ACAGTGGCGTCCAGGAGA ACTTT-3'
	3'-AACCT AAAAGACTGTGTTTGGGT        AGTCCTAGACCT        TGTCACCGCAGGTCCTCT TGAAA-5'
	                                                       TGACACCCCACCTCCCCT
	                                                            Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 012-003414995
Forward Primer: 5'-TGACGTTCCCTGGGCCAAGA-3' Tm: 59.508
Reverse Primer: 5'-AAAATCCAGTTCCTTGCTCGCACC-3' Tm: 59.038
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 132, 138
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr12:3415101-3415148

Rank: 34	Homology Score: 5
	Genomic Region: Intergenic	Closest Gene: MRM1
Match Type: L25R
	           Left Nuclease
	         TTTTCTGTCACCAATCCT
	5'-TTCTT TTATCTGCCCCCAACACT CCAGGCATTATGTGCATTCATAAAA  AAGGGGGGGTGGAGTGGA GAGAA-3'
	3'-AAGAA AATAGACGGGGGTTGTGA GGTCCGTAATACACGTAAGTATTTT  TTCCCCCCCACCTCACCT CTCTT-5'
	                                                       TGACACCCCACCTCCCCT
	                                                            Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 017-035084330
Forward Primer: 5'-GAGCCTCTGCTGGTTGGATG-3' Tm: 59.508
Reverse Primer: 5'-ATGTTGGGTGCTGGGGAAGG-3' Tm: 59.508
Expected PCR Product Length: 287
Expected CelI Cleavage Product Sizes: 131, 156
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr17:35084429-35084489

Rank: 35	Homology Score: 5
	Genomic Region: Intergenic	Closest Gene: MIR802
Match Type: L21R
	           Left Nuclease
	         TTTTCTGTCACCAATCCT
	5'-CAGAG TCCTTTCCCACCAATCCT   TCATCAGCCTAATCAATACTA    GCAGTGGGGTGGAAGGAA GATGA-3'
	3'-GTCTC AGGAAAGGGTGGTTAGGA   AGTAGTCGGATTAGTTATGAT    CGTCACCCCACCTTCCTT CTACT-5'
	                                                       TGACACCCCACCTCCCCT
	                                                            Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 021-036773953
Forward Primer: 5'-CCAGAGCGGTTTTCCAAGATGC-3' Tm: 59.258
Reverse Primer: 5'-GGATTCACAACACCATGGATGGAC-3' Tm: 59.038
Expected PCR Product Length: 371
Expected CelI Cleavage Product Sizes: 175, 196
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr21:36774098-36774154

Rank: 36	Homology Score: 5
	Genomic Region: Intergenic	Closest Gene: MOBP
Match Type: L13R
	           Left Nuclease
	         TTTTCTGTCACCAATCCT
	5'-TAGAT TTTTTTGTAAGCAATCTT       TTGAGACACAAGA        AGTGGGGTGTAGAGAGGA ATTCA-3'
	3'-ATCTA AAAAAACATTCGTTAGAA       AACTCTGTGTTCT        TCACCCCACATCTCTCCT TAAGT-5'
	                                                       TGACACCCCACCTCCCCT
	                                                            Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 003-039683322
Forward Primer: 5'-GGTCTCCAGGGAGCATTATACAG-3' Tm: 59.148
Reverse Primer: 5'-CTAGACACAGAGCCTTCAGTAAGC-3' Tm: 59.038
Expected PCR Product Length: 410
Expected CelI Cleavage Product Sizes: 229, 181
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr3:39683525-39683573

Rank: 37	Homology Score: 5
	Genomic Region: Intergenic	Closest Gene: C20orf197
Match Type: R16L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-GCACC TCCCATCAACCCCATAGC      AGGAATGGGGCGAACT      TTGATTGATGTAAGAAAA TGAAA-3'
	3'-CGTGG AGGGTAGTTGGGGTATCG      TCCTTACCCCGCTTGA      AACTAACTACATTCTTTT ACTTT-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 020-058618528
Forward Primer: 5'-GGGAGCTATGATCATGCCATAGG-3' Tm: 59.148
Reverse Primer: 5'-GCTCCATGTCATTGTTCTGGGC-3' Tm: 59.258
Expected PCR Product Length: 290
Expected CelI Cleavage Product Sizes: 135, 155
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr20:58618635-58618686

Rank: 38	Homology Score: 5
	Genomic Region: Intergenic	Closest Gene: CD6
Match Type: R22L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-TGGCT TCCCCTCCACTCCTGGGA   GACCTCATTATAATACGCCTCA   AGGATCTGGGACAGGAAA CACAA-3'
	3'-ACCGA AGGGGAGGTGAGGACCCT   CTGGAGTAATATTATGCGGAGT   TCCTAGACCCTGTCCTTT GTGTT-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 011-060790178
Forward Primer: 5'-GCCTGTGCTGAGACTGGGAA-3' Tm: 59.508
Reverse Primer: 5'-AGCTGTCAGTCAAATTGCTGGGTG-3' Tm: 59.038
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 131, 139
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr11:60790278-60790335

Rank: 39	Homology Score: 5
	Genomic Region: Intergenic	Closest Gene: SMAD3
Match Type: R13L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-TGTTC TTCCACCCAGCCCTCAGT       GCTCTGCTGAGGC        AGGATGGGTGGCTGGAAA AATGG-3'
	3'-ACAAG AAGGTGGGTCGGGAGTCA       CGAGACGACTCCG        TCCTACCCACCGACCTTT TTACC-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 015-067305574
Forward Primer: 5'-CCCCACCTTGCTGAATGGAC-3' Tm: 59.508
Reverse Primer: 5'-CCAGTCCTTGATCTCTTCCTGAGT-3' Tm: 59.038
Expected PCR Product Length: 275
Expected CelI Cleavage Product Sizes: 142, 133
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr15:67305690-67305738

Rank: 40	Homology Score: 5
	Genomic Region: Intergenic	Closest Gene: PJA1
Match Type: R21L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-GAGGA TCTCCTCCAGGCCACAGC   CCTACCTCACTCTGCAGTAGC    AGCAATGAGGAAAGAAAA ATGGT-3'
	3'-CTCCT AGAGGAGGTCCGGTGTCG   GGATGGAGTGAGACGTCATCG    TCGTTACTCCTTTCTTTT TACCA-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 00X-068408586
Forward Primer: 5'-GCACTCCTTTACCCTCCGATATC-3' Tm: 59.148
Reverse Primer: 5'-AGGTTTGAGGTGCACTACTTCAGG-3' Tm: 59.038
Expected PCR Product Length: 273
Expected CelI Cleavage Product Sizes: 142, 131
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chrX:68408698-68408754

Rank: 41	Homology Score: 2
	Genomic Region: Exon	Closest Gene: PAX8
Match Type: L25R
	           Left Nuclease
	         TTTTCTGTCACCAATCCT
	5'-TCTCC TTACCTGCCACCATGCCT GCGATGGCAGAGGAGGCATAGCTGC  CCTGTCCGCTGGTGGGGA TGTGG-3'
	3'-AGAGG AATGGACGGTGGTACGGA CGCTACCGTCTCCTCCGTATCGACG  GGACAGGCGACCACCCCT ACACC-5'
	                                                       TGACACCCCACCTCCCCT
	                                                            Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 002-113984626
Forward Primer: 5'-GGGAAGTCTATGAATAGGGCACAG-3' Tm: 59.038
Reverse Primer: 5'-TGATACATATGGGAGCCCCCATGG-3' Tm: 60.908
Expected PCR Product Length: 265
Expected CelI Cleavage Product Sizes: 134, 131
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr2:113984728-113984788

Rank: 42	Homology Score: 2
	Genomic Region: Exon	Closest Gene: HMCN1
Match Type: R23L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-AATTT TTCTCTCCACTCAACAGG  GTGGTGGTGATTGCCCAGAAATG   AGTATTGGAGCTATAAAA ATTGC-3'
	3'-TTAAA AAGAGAGGTGAGTTGTCC  CACCACCACTAACGGGTCTTTAC   TCATAACCTCGATATTTT TAACG-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 001-185833320
Forward Primer: 5'-CATCGTGTAAGAGAAGGCAGAGTG-3' Tm: 59.038
Reverse Primer: 5'-CTGTTGGATAAGTTGCAGCACCTC-3' Tm: 59.038
Expected PCR Product Length: 429
Expected CelI Cleavage Product Sizes: 296, 133
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr1:185833585-185833643

Rank: 43	Homology Score: 2
	Genomic Region: Exon	Closest Gene: PIP4K2B
Match Type: R26L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-TCCAA TGCCCTCGACTGCCCAGT AAAAGAATGGCGGGACCCAAGGAGGA AAGAGTGGGGGCAGACAA CGCTT-3'
	3'-AGGTT ACGGGAGCTGACGGGTCA TTTTCTTACCGCCCTGGGTTCCTCCT TTCTCACCCCCGTCTGTT GCGAA-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 017-036923296
Forward Primer: 5'-CCTTTCTTCTTGGGAGACCCAAC-3' Tm: 59.148
Reverse Primer: 5'-GAGTTTGGCCCCCAATTTTTGGCA-3' Tm: 59.038
Expected PCR Product Length: 312
Expected CelI Cleavage Product Sizes: 139, 173
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr17:36923402-36923463

Rank: 44	Homology Score: 2
	Genomic Region: Exon	Closest Gene: SKIV2L2
Match Type: R24L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-TGTAT TCTCATCCACTTCACAAT  GATCCAAATTTGGAAACTGTGTAT  ACGCTTTGTGAAAAAAAA GCACA-3'
	3'-ACATA AGAGTAGGTGAAGTGTTA  CTAGGTTTAAACCTTTGACACATA  TGCGAAACACTTTTTTTT CGTGT-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 005-054696084
Forward Primer: 5'-CGGCATCCCCTTATTAGACCC-3' Tm: 59.378
Reverse Primer: 5'-CTGACAGGTTCAGAGCTGACAGT-3' Tm: 59.148
Expected PCR Product Length: 317
Expected CelI Cleavage Product Sizes: 132, 185
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr5:54696184-54696243

Rank: 45	Homology Score: 2
	Genomic Region: Promoter	Closest Gene: ODF4
Match Type: R20L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-TCACC TCCCATCACCACCACACT    GGGGAGCAAATTTCAGCATG    AGGTTTGGTGAAGACAAA TTAAC-3'
	3'-AGTGG AGGGTAGTGGTGGTGTGA    CCCCTCGTTTAAAGTCGTAC    TCCAAACCACTTCTGTTT AATTG-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 017-008242168
Forward Primer: 5'-CAGCTATTGCGGGGAGCTAATG-3' Tm: 59.258
Reverse Primer: 5'-AGGCCACAGCTCAGAACTGTGGA-3' Tm: 61.088
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 136, 134
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr17:8242274-8242329

Rank: 46	Homology Score: 2
	Genomic Region: Intron	Closest Gene: SLC9A2
Match Type: L25R
	           Left Nuclease
	         TTTTCTGTCACCAATCCT
	5'-TTTCC TTCTCTGACACCACCCCA ATAGAAAAGCCAGGGTGTTCCTTAC  ACTCTGGTGAGGATGGAA TCTAA-3'
	3'-AAAGG AAGAGACTGTGGTGGGGT TATCTTTTCGGTCCCACAAGGAATG  TGAGACCACTCCTACCTT AGATT-5'
	                                                       TGACACCCCACCTCCCCT
	                                                            Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 002-103259635
Forward Primer: 5'-GTTCTCTGCGTTCTTTACGTCGTC-3' Tm: 59.038
Reverse Primer: 5'-GTCAACCTCACAAGGCACAGAAG-3' Tm: 59.148
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 130, 140
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr2:103259733-103259793

Rank: 47	Homology Score: 2
	Genomic Region: Intron	Closest Gene: GSTCD
Match Type: R12L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-TACTT TCTCTTCCATCAAACAGT        TGAAATATAGTG        AGGTATAGTGACCTAAAA ATGTT-3'
	3'-ATGAA AGAGAAGGTAGTTTGTCA        ACTTTATATCAC        TCCATATCACTGGATTTT TACAA-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 004-106759798
Forward Primer: 5'-GAGACCCACAGAGAATTGTCTTCC-3' Tm: 59.038
Reverse Primer: 5'-AGGTGTGAGTAGCACATAAGGTGG-3' Tm: 59.038
Expected PCR Product Length: 450
Expected CelI Cleavage Product Sizes: 320, 130
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr4:106760092-106760139

Rank: 48	Homology Score: 2
	Genomic Region: Intron	Closest Gene: TSC22D3
Match Type: R18L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-TGCTT TCCCCTCCCCCGAAGATT     CCAAGGAAGATGATGAGA     AGAAAAGGGGACAGAGAA ATAAG-3'
	3'-ACGAA AGGGGAGGGGGCTTCTAA     GGTTCCTTCTACTACTCT     TCTTTTCCCCTGTCTCTT TATTC-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 00X-106962065
Forward Primer: 5'-CTCTCTGAACACGGTGTTTGCACT-3' Tm: 59.038
Reverse Primer: 5'-TGCCTCTGTCTCCACATAAGGG-3' Tm: 59.258
Expected PCR Product Length: 405
Expected CelI Cleavage Product Sizes: 275, 130
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chrX:106962311-106962364

Rank: 49	Homology Score: 2
	Genomic Region: Intron	Closest Gene: SOBP
Match Type: R20L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-TGTGC TGCCTTTCCCCCCACAGA    GACAGCCTCTGGCCCTTGGT    ATGGTTTGTGACAGCACA GGCAT-3'
	3'-ACACG ACGGAAAGGGGGGTGTCT    CTGTCGGAGACCGGGAACCA    TACCAAACACTGTCGTGT CCGTA-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 006-107832934
Forward Primer: 5'-CCCTTTTCTCTGGAGAAGGGGA-3' Tm: 59.258
Reverse Primer: 5'-GGCTTGCCTATATGGCAAAGCCT-3' Tm: 59.148
Expected PCR Product Length: 334
Expected CelI Cleavage Product Sizes: 131, 203
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr6:107833035-107833090

Rank: 50	Homology Score: 2
	Genomic Region: Intron	Closest Gene: ANGPT1
Match Type: R12L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-ATCTC TTCCCTGCTCCCCACCAT        TCAGAATTTGTT        GTAATTTGTGAAAGAAAA GTGAA-3'
	3'-TAGAG AAGGGACGAGGGGTGGTA        AGTCTTAAACAA        CATTAAACACTTTCTTTT CACTT-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 008-108496180
Forward Primer: 5'-GATGGGAAAGATGCCTAGGAAGC-3' Tm: 59.148
Reverse Primer: 5'-GGAAACTGAGGGCCACTAAAGTC-3' Tm: 59.148
Expected PCR Product Length: 339
Expected CelI Cleavage Product Sizes: 186, 153
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr8:108496340-108496387

Rank: 51	Homology Score: 2
	Genomic Region: Intron	Closest Gene: DPP10
Match Type: L19R
	           Left Nuclease
	         TTTTCTGTCACCAATCCT
	5'-ATTGT TTTACTGTCTTAATTCCT    GTATATATGACACAGAAGG     GCGGTGGGGTGAAAGGAA GATGT-3'
	3'-TAACA AAATGACAGAATTAAGGA    CATATATACTGTGTCTTCC     CGCCACCCCACTTTCCTT CTACA-5'
	                                                       TGACACCCCACCTCCCCT
	                                                            Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 002-115914630
Forward Primer: 5'-GTGGGTAAACATGACAACAGCAGG-3' Tm: 59.038
Reverse Primer: 5'-CTTGCTCTCTGGAGACCTGAAGAT-3' Tm: 59.038
Expected PCR Product Length: 283
Expected CelI Cleavage Product Sizes: 144, 139
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr2:115914745-115914799

Rank: 52	Homology Score: 2
	Genomic Region: Intron	Closest Gene: KIRREL3
Match Type: R13L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-GTGTT TTCACTCACCACCACAGT       CAGGGCTGGCACA        GGGACTGGGGAATGAAAA ATAAA-3'
	3'-CACAA AAGTGAGTGGTGGTGTCA       GTCCCGACCGTGT        CCCTGACCCCTTACTTTT TATTT-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 011-126543310
Forward Primer: 5'-CCAGCAGCTCTGGAAATTGGATC-3' Tm: 59.148
Reverse Primer: 5'-GAGGAAGATCTTACAGACCCAACC-3' Tm: 59.038
Expected PCR Product Length: 345
Expected CelI Cleavage Product Sizes: 187, 158
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr11:126543471-126543519

Rank: 53	Homology Score: 2
	Genomic Region: Intron	Closest Gene: PVT1
Match Type: L24R
	           Left Nuclease
	         TTTTCTGTCACCAATCCT
	5'-ATAAA TTTCCTGACACCAAAAGT  CAGTTCAAGTGAACTAGAATGGGC  AGAGAGGGGTCGTGGGGA TGGTT-3'
	3'-TATTT AAAGGACTGTGGTTTTCA  GTCAAGTTCACTTGATCTTACCCG  TCTCTCCCCAGCACCCCT ACCAA-5'
	                                                       TGACACCCCACCTCCCCT
	                                                            Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 008-129090046
Forward Primer: 5'-CATCCTCCCAGCCATCAATACC-3' Tm: 59.258
Reverse Primer: 5'-AGGCTGGGACCACAGGCATA-3' Tm: 59.508
Expected PCR Product Length: 304
Expected CelI Cleavage Product Sizes: 174, 130
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr8:129090188-129090247

Rank: 54	Homology Score: 2
	Genomic Region: Intron	Closest Gene: TMEM132D
Match Type: L26R
	           Left Nuclease
	         TTTTCTGTCACCAATCCT
	5'-GTTGC TTTTATGCCACAAACACT AATTGCATTCCTATTGTTTGCTAAGC ACTGTGCTGAGGACAGGA GACAC-3'
	3'-CAACG AAAATACGGTGTTTGTGA TTAACGTAAGGATAACAAACGATTCG TGACACGACTCCTGTCCT CTGTG-5'
	                                                       TGACACCCCACCTCCCCT
	                                                            Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 012-129882479
Forward Primer: 5'-GGCCAAAACCTCTCTACATGGG-3' Tm: 59.258
Reverse Primer: 5'-CAGGGAGGAAAACAGGCACTAAAG-3' Tm: 59.038
Expected PCR Product Length: 373
Expected CelI Cleavage Product Sizes: 138, 235
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr12:129882584-129882645

Rank: 55	Homology Score: 2
	Genomic Region: Intron	Closest Gene: TMEM132D
Match Type: R12L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-GGGGT TTCTCTCCACCCCATATC        CCAAACCCTCTT        AGATTTGCTGAGAGAGAA TTTTG-3'
	3'-CCCCA AAGAGAGGTGGGGTATAG        GGTTTGGGAGAA        TCTAAACGACTCTCTCTT AAAAC-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 012-129962958
Forward Primer: 5'-CCACCATCTCATGTATGGTTGAGC-3' Tm: 59.038
Reverse Primer: 5'-GGGGATCAGAGCACATTCCAG-3' Tm: 59.378
Expected PCR Product Length: 337
Expected CelI Cleavage Product Sizes: 207, 130
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr12:129963139-129963186

Rank: 56	Homology Score: 2
	Genomic Region: Intron	Closest Gene: GPC4
Match Type: R25L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-TGGAA TCCCCTGCACCCAGCATA CTGCTCTGGATACAGGAGCCATTCA  ATGATTGCTGATAATAAA TTTCT-3'
	3'-ACCTT AGGGGACGTGGGTCGTAT GACGAGACCTATGTCCTCGGTAAGT  TACTAACGACTATTATTT AAAGA-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 00X-132539743
Forward Primer: 5'-CTGCCATAGACTGGGAAACTGGA-3' Tm: 59.148
Reverse Primer: 5'-CCAGAACACAGCTGGGAATGTGGA-3' Tm: 60.908
Expected PCR Product Length: 283
Expected CelI Cleavage Product Sizes: 152, 131
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chrX:132539863-132539923

Rank: 57	Homology Score: 2
	Genomic Region: Intron	Closest Gene: ABL1
Match Type: R26L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-GTCGT TGCCCATGGCCCCACAGT GAGTGGCAGATCTGAGACTCAGCGCT AGCCTTTGTGACACATAA CTCGT-3'
	3'-CAGCA ACGGGTACCGGGGTGTCA CTCACCGTCTAGACTCTGAGTCGCGA TCGGAAACACTGTGTATT GAGCA-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 009-133706117
Forward Primer: 5'-GGCACTCTCCCTACATGATTCC-3' Tm: 59.258
Reverse Primer: 5'-GTGGGTCCAGTGTATGTCCAAC-3' Tm: 59.258
Expected PCR Product Length: 322
Expected CelI Cleavage Product Sizes: 147, 175
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr9:133706231-133706292

Rank: 58	Homology Score: 2
	Genomic Region: Intron	Closest Gene: MGAT5
Match Type: L12R
	           Left Nuclease
	         TTTTCTGTCACCAATCCT
	5'-TGCCC TTTTCTGTCCAGAATCTG        CTGACTGGGACA        ACTGCAGGGTGGAAGCCA CCTTT-3'
	3'-ACGGG AAAAGACAGGTCTTAGAC        GACTGACCCTGT        TGACGTCCCACCTTCGGT GGAAA-5'
	                                                       TGACACCCCACCTCCCCT
	                                                            Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 002-135020983
Forward Primer: 5'-GGGCACAAGTTCTGGTGAAAGC-3' Tm: 59.258
Reverse Primer: 5'-GTCATGTAAGGCAATACCCACCAG-3' Tm: 59.038
Expected PCR Product Length: 278
Expected CelI Cleavage Product Sizes: 142, 136
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr2:135021099-135021146

Rank: 59	Homology Score: 2
	Genomic Region: Intron	Closest Gene: PDE7B
Match Type: L21R
	           Left Nuclease
	         TTTTCTGTCACCAATCCT
	5'-ACTCT TTTTCTTTTAACAGTTCT   TTTAACTTTTGTAGCTACTGA    ACTTTGGGGTTTGGGGTA TCAAT-3'
	3'-TGAGA AAAAGAAAATTGTCAAGA   AAATTGAAAACATCGATGACT    TGAAACCCCAAACCCCAT AGTTA-5'
	                                                       TGACACCCCACCTCCCCT
	                                                            Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 006-136482738
Forward Primer: 5'-GGGAGGGAACCCATTGCAGT-3' Tm: 59.508
Reverse Primer: 5'-CTATTGAGGGCAGGGAGCGT-3' Tm: 59.508
Expected PCR Product Length: 289
Expected CelI Cleavage Product Sizes: 158, 131
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr6:136482866-136482922

Rank: 60	Homology Score: 2
	Genomic Region: Intron	Closest Gene: FAM13B
Match Type: R15L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-AAAAA TACCATCAACCCAACAGA      AAAATGTGGATGTGG       ACAACTGGAAACAGAAAA GACGA-3'
	3'-TTTTT ATGGTAGTTGGGTTGTCT      TTTTACACCTACACC       TGTTGACCTTTGTCTTTT CTGCT-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 005-137303124
Forward Primer: 5'-ACTGCTTGAACCCAGGAGGC-3' Tm: 59.508
Reverse Primer: 5'-CTTTGTTGCCTGGGCTGGTC-3' Tm: 59.508
Expected PCR Product Length: 278
Expected CelI Cleavage Product Sizes: 147, 131
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr5:137303244-137303294

Rank: 61	Homology Score: 2
	Genomic Region: Intron	Closest Gene: FAM107B
Match Type: L13R
	           Left Nuclease
	         TTTTCTGTCACCAATCCT
	5'-TTTTT TTTTTTGAGACAAAACCT       CACTCTGTCACCC        AGGCTGGAGTGCAGGGGA GCAAT-3'
	3'-AAAAA AAAAAACTCTGTTTTGGA       GTGAGACAGTGGG        TCCGACCTCACGTCCCCT CGTTA-5'
	                                                       TGACACCCCACCTCCCCT
	                                                            Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 010-014726614
Forward Primer: 5'-GCATTGTGCCAAAAGGATCCCG-3' Tm: 59.258
Reverse Primer: 5'-GATAGGGAGACCCCATGTCTAC-3' Tm: 59.258
Expected PCR Product Length: 419
Expected CelI Cleavage Product Sizes: 244, 175
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr10:14726832-14726880

Rank: 62	Homology Score: 2
	Genomic Region: Intron	Closest Gene: LYPD6B
Match Type: R23L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-CCTTA TCTCCTCCTTCCTAAAGT  AAGGTCTTTAGATATTCTTTTGA   AGTTTTGTTGATAGTAAA CTCCT-3'
	3'-GGAAT AGAGGAGGAAGGATTTCA  TTCCAGAAATCTATAAGAAAACT   TCAAAACAACTATCATTT GAGGA-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 002-149982161
Forward Primer: 5'-GGCATCTTCGAGTACCTTAACTCC-3' Tm: 59.038
Reverse Primer: 5'-CTCTTCTCAAGTTCAAGGGTCAGTTGTAC-3' Tm: 60.178
Expected PCR Product Length: 409
Expected CelI Cleavage Product Sizes: 219, 190
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr2:149982349-149982407

Rank: 63	Homology Score: 2
	Genomic Region: Intron	Closest Gene: RXFP1
Match Type: L15R
	           Left Nuclease
	         TTTTCTGTCACCAATCCT
	5'-TATAT TTATATTTCAGCAATCGT      CTCAATTACATCCTT       TATGCGGGGTGGCCGGGA GGAAT-3'
	3'-ATATA AATATAAAGTCGTTAGCA      GAGTTAATGTAGGAA       ATACGCCCCACCGGCCCT CCTTA-5'
	                                                       TGACACCCCACCTCCCCT
	                                                            Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 004-159462895
Forward Primer: 5'-GCAAGCACTTGAGCATGTATCTCC-3' Tm: 59.038
Reverse Primer: 5'-AACCCAGGAGGTGGAGGTTG-3' Tm: 59.508
Expected PCR Product Length: 275
Expected CelI Cleavage Product Sizes: 136, 139
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr4:159463004-159463054

Rank: 64	Homology Score: 2
	Genomic Region: Intron	Closest Gene: MPHOSPH8
Match Type: R22L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-ATCCC TTCTCTCTACCCCCAAGT   CATTTAGGATAAGGTGGTTTTG   GGAAATGGGAACAGAAAA TACTT-3'
	3'-TAGGG AAGAGAGATGGGGGTTCA   GTAAATCCTATTCCACCAAAAC   CCTTTACCCTTGTCTTTT ATGAA-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 013-020219809
Forward Primer: 5'-TCGCTTGAACCCAGGAGGCA-3' Tm: 59.508
Reverse Primer: 5'-CCCTGGATCCTGGAATGTCCT-3' Tm: 59.378
Expected PCR Product Length: 373
Expected CelI Cleavage Product Sizes: 141, 232
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr13:20219919-20219976

Rank: 65	Homology Score: 2
	Genomic Region: Intron	Closest Gene: C17orf103
Match Type: R25L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-CTGCA TCCCCACCCCACCACATC CCTGGATCTGGGAGTCCCAGAACCC  AGCACTGGACACAGAACA GGGCT-3'
	3'-GACGT AGGGGTGGGGTGGTGTAG GGACCTAGACCCTCAGGGTCTTGGG  TCGTGACCTGTGTCTTGT CCCGA-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 017-021147767
Forward Primer: 5'-CCATGCCTAGAACACTTGAGCC-3' Tm: 59.258
Reverse Primer: 5'-CTTTCCTGCTGCGTCTCTCTC-3' Tm: 59.378
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 132, 138
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr17:21147867-21147927

Rank: 66	Homology Score: 2
	Genomic Region: Intron	Closest Gene: CDKAL1
Match Type: L17R
	           Left Nuclease
	         TTTTCTGTCACCAATCCT
	5'-TCCCC TTTTATGTCAACAATTAA     AAAATGTTATATACAGT      ATTGTTGGGTGTAGGTAA GAAAA-3'
	3'-AGGGG AAAATACAGTTGTTAATT     TTTTACAATATATGTCA      TAACAACCCACATCCATT CTTTT-5'
	                                                       TGACACCCCACCTCCCCT
	                                                            Right Nuclease
Warning! Primer pair was generated using suboptimal parameters. 
Primer Index: 006-021174562
Forward Primer: 5'-GTCGGTATAATCTTAGTCAAAAGTTCAACAGGG-3' Tm: 59.758
Reverse Primer: 5'-TAGTTAAATTGCCCTCCAAAATGGCCTTATCAATG-3' Tm: 59.578
Expected PCR Product Length: 406
Expected CelI Cleavage Product Sizes: 275, 131
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr6:21174809-21174861

Rank: 67	Homology Score: 2
	Genomic Region: Intron	Closest Gene: PARP1
Match Type: R17L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-ACATC TCCTCTCCATCACACACA     GCATACACTGGGACACC      AGGAATGGAGACATGAGA GGATG-3'
	3'-TGTAG AGGAGAGGTAGTGTGTGT     CGTATGTGACCCTGTGG      TCCTTACCTCTGTACTCT CCTAC-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 001-226575130
Forward Primer: 5'-GGAGACAAAAATGCCAGCCCTATC-3' Tm: 59.038
Reverse Primer: 5'-CCACTGTGGCACATCTGTGTC-3' Tm: 59.378
Expected PCR Product Length: 300
Expected CelI Cleavage Product Sizes: 169, 131
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr1:226575271-226575323

Rank: 68	Homology Score: 2
	Genomic Region: Intron	Closest Gene: NCMAP
Match Type: R17L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-CCTTG TCCCCTCCCCACCCAAGC     ATCTGGCGGAGGGGCCG      AGGCTCGCTGGAAGAAAA GGGAG-3'
	3'-GGAAC AGGGGAGGGGTGGGTTCG     TAGACCGCCTCCCCGGC      TCCGAGCGACCTTCTTTT CCCTC-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 001-024882812
Forward Primer: 5'-CATCTGTTGGAGGAAGTGCCTG-3' Tm: 59.258
Reverse Primer: 5'-CTCTGCATAGGGCAGACCCA-3' Tm: 59.508
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 137, 133
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr1:24882921-24882973

Rank: 69	Homology Score: 2
	Genomic Region: Intron	Closest Gene: MYO18B
Match Type: L17R
	           Left Nuclease
	         TTTTCTGTCACCAATCCT
	5'-TTGGC TGCTCTGTGGCTAATCCT     CACATATCCCTTCTGAT      CATGTCGGCTGGTGGGGA TATGA-3'
	3'-AACCG ACGAGACACCGATTAGGA     GTGTATAGGGAAGACTA      GTACAGCCGACCACCCCT ATACT-5'
	                                                       TGACACCCCACCTCCCCT
	                                                            Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 022-026293769
Forward Primer: 5'-GTTTAAGGCCGTGTGCATTCTGG-3' Tm: 59.148
Reverse Primer: 5'-GTTTGCTCAGAGGTAAAGTGGGG-3' Tm: 59.148
Expected PCR Product Length: 320
Expected CelI Cleavage Product Sizes: 149, 171
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr22:26293890-26293942

Rank: 70	Homology Score: 2
	Genomic Region: Intron	Closest Gene: MYO1D
Match Type: R24L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-TATTG TCCTCAGCACACAACAGT  ACCTATACAGTAGAATAAGAACCA  AAGATTGTTTATACAAAA ATTAA-3'
	3'-ATAAC AGGAGTCGTGTGTTGTCA  TGGATATGTCATCTTATTCTTGGT  TTCTAACAAATATGTTTT TAATT-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 017-031174782
Forward Primer: 5'-CTCAGCCTCCCAAAATGCTGAGA-3' Tm: 59.148
Reverse Primer: 5'-CGGAGAGAGGCCCATTTTTCATAG-3' Tm: 59.038
Expected PCR Product Length: 344
Expected CelI Cleavage Product Sizes: 144, 200
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr17:31174894-31174953

Rank: 71	Homology Score: 2
	Genomic Region: Intron	Closest Gene: DIP2C
Match Type: L17R
	           Left Nuclease
	         TTTTCTGTCACCAATCCT
	5'-CTTCT TTTTCTGTTTCTCCTCCT     GCTGGCTGGAATGTGGA      AATGATGGCTGGAGCGGA AGCAG-3'
	3'-GAAGA AAAAGACAAAGAGGAGGA     CGACCGACCTTACACCT      TTACTACCGACCTCGCCT TCGTC-5'
	                                                       TGACACCCCACCTCCCCT
	                                                            Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 010-000333834
Forward Primer: 5'-TCCCAACCTTTAGCTAGGTGTGGC-3' Tm: 60.908
Reverse Primer: 5'-TAGGCATCTCCTCCTCCATGAAG-3' Tm: 59.148
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 137, 133
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr10:333943-333995

Rank: 72	Homology Score: 2
	Genomic Region: Intron	Closest Gene: RNU6-76
Match Type: L26R
	           Left Nuclease
	         TTTTCTGTCACCAATCCT
	5'-CCTAC TTTTCTCTAGCCACTGCT CATTCTCCTTTACCAATTCTTTTTCT CCTGGGGGCGGGAGGGAA TGTTG-3'
	3'-GGATG AAAAGAGATCGGTGACGA GTAAGAGGAAATGGTTAAGAAAAAGA GGACCCCCGCCCTCCCTT ACAAC-5'
	                                                       TGACACCCCACCTCCCCT
	                                                            Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 016-033560648
Forward Primer: 5'-GTTCTTTTGAAAAGGGTACTGCCAAAGAGC-3' Tm: 60.058
Reverse Primer: 5'-GGTTGGAGATGCAGACGTAGAATG-3' Tm: 59.038
Expected PCR Product Length: 336
Expected CelI Cleavage Product Sizes: 182, 154
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr16:33560797-33560858

Rank: 73	Homology Score: 2
	Genomic Region: Intron	Closest Gene: RYR3
Match Type: R24L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-CTCAA TCCCCATCATCCTACAGA  AATGACTTTAAAGTGAGAGCAAAC  AGCATTTGTGATGAAAAA AATGC-3'
	3'-GAGTT AGGGGTAGTAGGATGTCT  TTACTGAAATTTCACTCTCGTTTG  TCGTAAACACTACTTTTT TTACG-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 015-033799697
Forward Primer: 5'-GGCACTGTGGCCAATTTTATGGCT-3' Tm: 59.038
Reverse Primer: 5'-CCCAGTCCTACGAGATAGATTGC-3' Tm: 59.148
Expected PCR Product Length: 361
Expected CelI Cleavage Product Sizes: 193, 168
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr15:33799858-33799917

Rank: 74	Homology Score: 2
	Genomic Region: Intron	Closest Gene: DUSP22
Match Type: R19L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-AGAAC TTCCCTCAACCCCAGTCT    GTGGTAACTATGTGTTTCA     GGGATTTCTTACAGAGAA ATTCC-3'
	3'-TCTTG AAGGGAGTTGGGGTCAGA    CACCATTGATACACAAAGT     CCCTAAAGAATGTCTCTT TAAGG-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 006-000344885
Forward Primer: 5'-ACCTTCCTCACCTGCGTCAC-3' Tm: 59.508
Reverse Primer: 5'-AACCTGGTCATACTGTCTGTGGC-3' Tm: 59.148
Expected PCR Product Length: 276
Expected CelI Cleavage Product Sizes: 145, 131
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr6:345001-345055

Rank: 75	Homology Score: 2
	Genomic Region: Intron	Closest Gene: VIT
Match Type: L21R
	           Left Nuclease
	         TTTTCTGTCACCAATCCT
	5'-ACACG TTCTTTGTAATCAACCCT   AGGATATCAAAATTGGAAACA    GTTGTGGGATGAAGGGAA CTGTT-3'
	3'-TGTGC AAGAAACATTAGTTGGGA   TCCTATAGTTTTAACCTTTGT    CAACACCCTACTTCCCTT GACAA-5'
	                                                       TGACACCCCACCTCCCCT
	                                                            Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 002-037018108
Forward Primer: 5'-TTTCAGCTGCATCCCGGACC-3' Tm: 59.508
Reverse Primer: 5'-AGGAGATGCGGCCAAGTCAG-3' Tm: 59.508
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 135, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr2:37018213-37018269

Rank: 76	Homology Score: 2
	Genomic Region: Intron	Closest Gene: THADA
Match Type: R25L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-ATTTG TTCACTTCAAACCACAGT AGCAAATAAGGAATGGATTCAAACA  AATATTGCTCTCAGAAAA TACCA-3'
	3'-TAAAC AAGTGAAGTTTGGTGTCA TCGTTTATTCCTTACCTAAGTTTGT  TTATAACGAGAGTCTTTT ATGGT-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 002-043471241
Forward Primer: 5'-GCCCACCTCAAAACACTGGGTA-3' Tm: 59.258
Reverse Primer: 5'-GTGAGGTGCACACAAAACTGGG-3' Tm: 59.258
Expected PCR Product Length: 378
Expected CelI Cleavage Product Sizes: 239, 139
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr2:43471448-43471508

Rank: 77	Homology Score: 2
	Genomic Region: Intron	Closest Gene: MAPT
Match Type: R24L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-TTCCT TACCTCCCACCCCACTGC  ATCTAAGTCACTGCCTGAGAAAAC  AGGATTGAGGAAAGGAGA AAGGA-3'
	3'-AAGGA ATGGAGGGTGGGGTGACG  TAGATTCAGTGACGGACTCTTTTG  TCCTAACTCCTTTCCTCT TTCCT-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 017-044029096
Forward Primer: 5'-GGACGGACTCAGGAGGCAAT-3' Tm: 59.508
Reverse Primer: 5'-CACACCAAGGCTGTTTTCCTCAGA-3' Tm: 59.038
Expected PCR Product Length: 266
Expected CelI Cleavage Product Sizes: 136, 130
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr17:44029200-44029259

Rank: 78	Homology Score: 2
	Genomic Region: Intron	Closest Gene: ITGB2
Match Type: R23L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-GGGAA TTCCTACCACCACACAGA  TGGTAGGCGGAAGTAGCCCCACG   AGGGTGGATCACAGTAAA ATCAC-3'
	3'-CCCTT AAGGATGGTGGTGTGTCT  ACCATCCGCCTTCATCGGGGTGC   TCCCACCTAGTGTCATTT TAGTG-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 021-046316063
Forward Primer: 5'-GATCCTGCCTGGTGGATTTCAAG-3' Tm: 59.148
Reverse Primer: 5'-ATGTGTGAGCATGGGGGAGG-3' Tm: 59.508
Expected PCR Product Length: 284
Expected CelI Cleavage Product Sizes: 130, 154
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr21:46316162-46316220

Rank: 79	Homology Score: 2
	Genomic Region: Intron	Closest Gene: NKD1
Match Type: R17L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-CTGAC TCTCCACTCCCCTACAGT     CCCCCCAGAAGCTGAGT      TGCAGCGGTGACAGAGAA CAAGC-3'
	3'-GACTG AGAGGTGAGGGGATGTCA     GGGGGGTCTTCGACTCA      ACGTCGCCACTGTCTCTT GTTCG-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 016-050647321
Forward Primer: 5'-TCATGGGAAGGCAGCAGGGA-3' Tm: 59.508
Reverse Primer: 5'-TAAAGCCTCTCCGGTATCTGACAC-3' Tm: 59.038
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 139, 131
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr16:50647432-50647484

Rank: 80	Homology Score: 2
	Genomic Region: Intron	Closest Gene: FERMT2
Match Type: R22L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-TATAG TACCCTACACCCTACTTT   CTAGCACAGAATTTGCCAACTA   ACTTTTGGAAACAGAAAA TTCTG-3'
	3'-ATATC ATGGGATGTGGGATGAAA   GATCGTGTCTTAAACGGTTGAT   TGAAAACCTTTGTCTTTT AAGAC-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 014-053388076
Forward Primer: 5'-GGTTTTGGCAAAGTCCTGCCAATC-3' Tm: 59.038
Reverse Primer: 5'-CCTACCGATCATTCAGTTCAGCTC-3' Tm: 59.038
Expected PCR Product Length: 324
Expected CelI Cleavage Product Sizes: 139, 185
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr14:53388184-53388241

Rank: 81	Homology Score: 2
	Genomic Region: Intron	Closest Gene: ERC2
Match Type: R22L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-AAATC TCACCTCCTGCCCAAAGC   TGATCTGTCTCCCTACTCTTCC   AGGCTTGGTTCCATGAAA AACCC-3'
	3'-TTTAG AGTGGAGGACGGGTTTCG   ACTAGACAGAGGGATGAGAAGG   TCCGAACCAAGGTACTTT TTGGG-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 003-056450116
Forward Primer: 5'-GGGAAAAGACGTGATTTGGCCAAC-3' Tm: 59.038
Reverse Primer: 5'-GGAGGTTAGCTTTGAAGGGTGG-3' Tm: 59.258
Expected PCR Product Length: 389
Expected CelI Cleavage Product Sizes: 233, 156
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr3:56450318-56450375

Rank: 82	Homology Score: 2
	Genomic Region: Intron	Closest Gene: ERBB2IP
Match Type: R16L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-GCAGC TCCGTTCCACCAGACTGT      CATGTCTGTGGGAGTG      TGTATGAGTGACAGAAGA TTTGG-3'
	3'-CGTCG AGGCAAGGTGGTCTGACA      GTACAGACACCCTCAC      ACATACTCACTGTCTTCT AAACC-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 005-065282040
Forward Primer: 5'-GCTGCTTACTCAGTGTCCTACTC-3' Tm: 59.148
Reverse Primer: 5'-GGTATAGGACTAACGTGTTTAACGTGTGTG-3' Tm: 60.058
Expected PCR Product Length: 303
Expected CelI Cleavage Product Sizes: 156, 147
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr5:65282168-65282219

Rank: 83	Homology Score: 2
	Genomic Region: Intron	Closest Gene: JAK1
Match Type: L24R
	           Left Nuclease
	         TTTTCTGTCACCAATCCT
	5'-AGACA TTTTTGGTCATCACTGCT  AAGGAGAGAGTGCTACTGGCATCT  ACTGGGTGGAGGCAGGGA TGCTG-3'
	3'-TCTGT AAAAACCAGTAGTGACGA  TTCCTCTCTCACGATGACCGTAGA  TGACCCACCTCCGTCCCT ACGAC-5'
	                                                       TGACACCCCACCTCCCCT
	                                                            Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 001-065337739
Forward Primer: 5'-TCTCTCCTTGCAGCTCTACTGG-3' Tm: 59.258
Reverse Primer: 5'-CTTCCATGTAGAGTGTCTCAGCAG-3' Tm: 59.038
Expected PCR Product Length: 269
Expected CelI Cleavage Product Sizes: 139, 130
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr1:65337846-65337905

Rank: 84	Homology Score: 2
	Genomic Region: Intron	Closest Gene: TYW1
Match Type: L25R
	           Left Nuclease
	         TTTTCTGTCACCAATCCT
	5'-AAGCT TCTTCTGACACCACTCAA GTGTATGGGGGAGAGGTGCCTTGTT  ACTGCTAGGTGGAGTGAA AGTCC-3'
	3'-TTCGA AGAAGACTGTGGTGAGTT CACATACCCCCTCTCCACGGAACAA  TGACGATCCACCTCACTT TCAGG-5'
	                                                       TGACACCCCACCTCCCCT
	                                                            Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 007-066670329
Forward Primer: 5'-TGACTGTTCCCCCCTTAGACTC-3' Tm: 59.258
Reverse Primer: 5'-TTGACTCCTACTACCAGGGCG-3' Tm: 59.378
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 135, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr7:66670432-66670492

Rank: 85	Homology Score: 2
	Genomic Region: Intron	Closest Gene: LRRC49
Match Type: R18L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-TAATT TACACTCCCACCAACAGT     GTAAAAGAACTTCACCCC     AGCCTGGGTGACAGAGCA AGACC-3'
	3'-ATTAA ATGTGAGGGTGGTTGTCA     CATTTTCTTGAAGTGGGG     TCGGACCCACTGTCTCGT TCTGG-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Warning! Primer pair was generated using suboptimal parameters. 
Primer Index: 015-071318700
Forward Primer: 5'-GTGCTGCAATAAACATACATGTGCATGTGTC-3' Tm: 59.958
Reverse Primer: 5'-CCCCATCAAAAAGTGGGTGAAGG-3' Tm: 59.148
Expected PCR Product Length: 425
Expected CelI Cleavage Product Sizes: 188, 237
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr15:71318859-71318912

Rank: 86	Homology Score: 2
	Genomic Region: Intron	Closest Gene: TYW1B
Match Type: R25L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-GGACT TTCACTCCACCTAGCAGT AACAAGGCACCTCTCCCCCATACAC  TCGAGTGGTGTCAGAAGA AGCTT-3'
	3'-CCTGA AAGTGAGGTGGATCGTCA TTGTTCCGTGGAGAGGGGGTATGTG  AGCTCACCACAGTCTTCT TCGAA-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 007-072071381
Forward Primer: 5'-ACTCCTACTACCAGGGCGGTAT-3' Tm: 59.258
Reverse Primer: 5'-TTGTGACTGTTCCCCCCTTAGAC-3' Tm: 59.148
Expected PCR Product Length: 270
Expected CelI Cleavage Product Sizes: 135, 135
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr7:72071484-72071544

Rank: 87	Homology Score: 2
	Genomic Region: Intron	Closest Gene: EXOC6B
Match Type: R23L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-AGAGT TCCCCTCCCCACCATAAG  GTGGGCAAAACACATGGCCAAAT   AAAAAAGGTGCCAGAAAA AGGCC-3'
	3'-TCTCA AGGGGAGGGGTGGTATTC  CACCCGTTTTGTGTACCGGTTTA   TTTTTTCCACGGTCTTTT TCCGG-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 002-072909053
Forward Primer: 5'-CCCTTCTCTCCACTAGCCAAGAAA-3' Tm: 59.038
Reverse Primer: 5'-CTTAAGAAGAATGAGGAAATGGTTGGAGCC-3' Tm: 60.058
Expected PCR Product Length: 351
Expected CelI Cleavage Product Sizes: 220, 131
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr2:72909242-72909300

Rank: 88	Homology Score: 2
	Genomic Region: Intron	Closest Gene: MAP6
Match Type: L23R
	           Left Nuclease
	         TTTTCTGTCACCAATCCT
	5'-ATTCT TTATCTGTCAGCTATGCA  GGTGGTCAGGCTGGCATCCTGAG   ACTGAGGGGGAGAGAAGA GAAGA-3'
	3'-TAAGA AATAGACAGTCGATACGT  CCACCAGTCCGACCGTAGGACTC   TGACTCCCCCTCTCTTCT CTTCT-5'
	                                                       TGACACCCCACCTCCCCT
	                                                            Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 011-075336327
Forward Primer: 5'-CATTCAGCTGAGCAAACAAAGCCC-3' Tm: 59.038
Reverse Primer: 5'-CCTCTCTGGGCTTTGGTTTTCTC-3' Tm: 59.148
Expected PCR Product Length: 282
Expected CelI Cleavage Product Sizes: 131, 151
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr11:75336427-75336485

Rank: 89	Homology Score: 2
	Genomic Region: Intron	Closest Gene: RORB
Match Type: R11L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-TTTGG TCCCTTCCACCTTTCAAT        TACCACTTGAC         AGTACATTTGACAGAAAA GGATG-3'
	3'-AAACC AGGGAAGGTGGAAAGTTA        ATGGTGAACTG         TCATGTAAACTGTCTTTT CCTAC-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 009-077165385
Forward Primer: 5'-GGTCTTATCGACTAGCTAGGGTTG-3' Tm: 59.038
Reverse Primer: 5'-GGGCTGCCCTTTAGATTTTGCTG-3' Tm: 59.148
Expected PCR Product Length: 280
Expected CelI Cleavage Product Sizes: 143, 137
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr9:77165503-77165549

Rank: 90	Homology Score: 2
	Genomic Region: Intron	Closest Gene: C16orf46
Match Type: L17R
	           Left Nuclease
	         TTTTCTGTCACCAATCCT
	5'-GTTTA TTTTCTTTTACCAATAAC     ATGTTTTGCTTCAAAAT      AATGTGAGGTTGAGGTTA GGGAG-3'
	3'-CAAAT AAAAGAAAATGGTTATTG     TACAAAACGAAGTTTTA      TTACACTCCAACTCCAAT CCCTC-5'
	                                                       TGACACCCCACCTCCCCT
	                                                            Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 016-081100645
Forward Primer: 5'-GCCCCCAAAGTGCATGTTTGGA-3' Tm: 59.258
Reverse Primer: 5'-GAGCTCTCCGATAACATCATGGC-3' Tm: 59.148
Expected PCR Product Length: 420
Expected CelI Cleavage Product Sizes: 244, 176
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr16:81100861-81100913

Rank: 91	Homology Score: 2
	Genomic Region: Intron	Closest Gene: MAPT
Match Type: L24R
	           Left Nuclease
	         TTTTCTGTCACCAATCCT
	5'-TCCTT TCTCCTTTCCTCAATCCT  GTTTTCTCAGGCAGTGACTTAGAT  GCAGTGGGGTGGGAGGTA AGGAA-3'
	3'-AGGAA AGAGGAAAGGAGTTAGGA  CAAAAGAGTCCGTCACTGAATCTA  CGTCACCCCACCCTCCAT TCCTT-5'
	                                                       TGACACCCCACCTCCCCT
	                                                            Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: ap1-000838311
Forward Primer: 5'-ACCAAGGCTGTTTTCCTCAGACC-3' Tm: 59.148
Reverse Primer: 5'-AACCACAGGGACGGACTCAG-3' Tm: 59.508
Expected PCR Product Length: 271
Expected CelI Cleavage Product Sizes: 131, 140
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr17_ctg5_hap1:838410-838469

Rank: 92	Homology Score: 2
	Genomic Region: Intron	Closest Gene: SNCA
Match Type: L18R
	           Left Nuclease
	         TTTTCTGTCACCAATCCT
	5'-TTCAA TTTTCTATTATCAATTCC     TAGTCTGCTCATAATGCC     ATTTTGGGGTGGGGAGCA AAGGG-3'
	3'-AAGTT AAAAGATAATAGTTAAGG     ATCAGACGAGTATTACGG     TAAAACCCCACCCCTCGT TTCCC-5'
	                                                       TGACACCCCACCTCCCCT
	                                                            Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 004-090656661
Forward Primer: 5'-CAGGGGAGTGTGTACATAGTGTAG-3' Tm: 59.038
Reverse Primer: 5'-CCAGAGTTGTTCCTCTCCACATAG-3' Tm: 59.038
Expected PCR Product Length: 359
Expected CelI Cleavage Product Sizes: 169, 190
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr4:90656801-90656854

Rank: 93	Homology Score: 2
	Genomic Region: Intron	Closest Gene: CHD2
Match Type: R19L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-AATAA TACCATCTACCCAACAGG    ATTATTAGAAACTATGCTA     AGATTTTGTGACATTAAA TAAAT-3'
	3'-TTATT ATGGTAGATGGGTTGTCC    TAATAATCTTTGATACGAT     TCTAAAACACTGTAATTT ATTTA-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 015-093472933
Forward Primer: 5'-GGTGTGGATCCCGTTGACAATCT-3' Tm: 59.148
Reverse Primer: 5'-GCCAAGTGCAGGTTTGCTTACAG-3' Tm: 59.148
Expected PCR Product Length: 406
Expected CelI Cleavage Product Sizes: 262, 144
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr15:93473166-93473220

Rank: 94	Homology Score: 2
	Genomic Region: Intron	Closest Gene: ANKRD36
Match Type: R26L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-AACAT TCCCTCCCAACCCCCAGG AAAAAAAGAGTTGGTAAAGGAGAATG AGCAGTGGCTAGAGAAAA CTAGG-3'
	3'-TTGTA AGGGAGGGTTGGGGGTCC TTTTTTTCTCAACCATTTCCTCTTAC TCGTCACCGATCTCTTTT GATCC-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 002-097793909
Forward Primer: 5'-GGTTGGAGATGCAGACGTAGAATG-3' Tm: 59.038
Reverse Primer: 5'-GAAAAGGGTACCGCCAAAGAGC-3' Tm: 59.258
Expected PCR Product Length: 334
Expected CelI Cleavage Product Sizes: 163, 171
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr2:97794039-97794100

Rank: 95	Homology Score: 2
	Genomic Region: Intron	Closest Gene: BLNK
Match Type: R14L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-TCTTG TCCCCTGCACCCAACCTC       CCTTCCTGTACCTG       AAGATAGATGCCAGACAA TCGTT-3'
	3'-AGAAC AGGGGACGTGGGTTGGAG       GGAAGGACATGGAC       TTCTATCTACGGTCTGTT AGCAA-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 010-097970211
Forward Primer: 5'-GGGCTTTCGCCGACAGGTTT-3' Tm: 59.508
Reverse Primer: 5'-AGGCAGCTAGGGGATGCTGA-3' Tm: 59.508
Expected PCR Product Length: 272
Expected CelI Cleavage Product Sizes: 131, 141
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr10:97970315-97970364

Rank: 96	Homology Score: 2
	Genomic Region: Intergenic	Closest Gene: FLJ25363
Match Type: L21R
	           Left Nuclease
	         TTTTCTGTCACCAATCCT
	5'-TTTAA TTTCCTTTCAGCAAACAT   TTATTGAGTGCCAAGCATTGA    ACTAGGTGTTGGAAGGGA ATACA-3'
	3'-AAATT AAAGGAAAGTCGTTTGTA   AATAACTCACGGTTCGTAACT    TGATCCACAACCTTCCCT TATGT-5'
	                                                       TGACACCCCACCTCCCCT
	                                                            Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 003-109619335
Forward Primer: 5'-CAGGGAAGTCTCAGTGGTTTTTGG-3' Tm: 59.038
Reverse Primer: 5'-CCAGGTGTATTCTGTGTGGCTAG-3' Tm: 59.148
Expected PCR Product Length: 421
Expected CelI Cleavage Product Sizes: 266, 155
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr3:109619571-109619627

Rank: 97	Homology Score: 2
	Genomic Region: Intergenic	Closest Gene: C4orf32
Match Type: R20L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-GATTT TCCTCTCCTCCCCATGAT    AAACTCTGCCTTCTACTAAT    AGTATCAGTTACAAAAAA TTAAC-3'
	3'-CTAAA AGGAGAGGAGGGGTACTA    TTTGAGACGGAAGATGATTA    TCATAGTCAATGTTTTTT AATTG-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 004-112705369
Forward Primer: 5'-CATCCTTACTCAGGGCTGTTTCATGATGT-3' Tm: 60.178
Reverse Primer: 5'-CTGGACAACAGACCTTTCCATGG-3' Tm: 59.148
Expected PCR Product Length: 285
Expected CelI Cleavage Product Sizes: 132, 153
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr4:112705471-112705526

Rank: 98	Homology Score: 2
	Genomic Region: Intergenic	Closest Gene: TAS2R42
Match Type: L16R
	           Left Nuclease
	         TTTTCTGTCACCAATCCT
	5'-GTATT TTTTCTTTTTCAAAACCT      ATGGGCTGTACTTTTC      CATGTGGGGTGATGGGTA ACAAG-3'
	3'-CATAA AAAAGAAAAAGTTTTGGA      TACCCGACATGAAAAG      GTACACCCCACTACCCAT TGTTC-5'
	                                                       TGACACCCCACCTCCCCT
	                                                            Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 012-011342495
Forward Primer: 5'-CTGTCTTCCAGTCTAAAGCAACTACCTC-3' Tm: 60.298
Reverse Primer: 5'-CCACACATCCTCTTCCTCCTATTC-3' Tm: 59.038
Expected PCR Product Length: 385
Expected CelI Cleavage Product Sizes: 132, 253
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr12:11342599-11342650

Rank: 99	Homology Score: 2
	Genomic Region: Intergenic	Closest Gene: UGT8
Match Type: L22R
	           Left Nuclease
	         TTTTCTGTCACCAATCCT
	5'-TATAG TTTTCTTTCAACATTCAC   TCAGGAAATGATTATGTGTGAG   TATGTGGATTTGAGGGGA AGTTA-3'
	3'-ATATC AAAAGAAAGTTGTAAGTG   AGTCCTTTACTAATACACACTC   ATACACCTAAACTCCCCT TCAAT-5'
	                                                       TGACACCCCACCTCCCCT
	                                                            Right Nuclease
Primer pair generated using optimal parameters. 
Primer Index: 004-115491385
Forward Primer: 5'-CATGTTGCACAGGCTGAGTACTCT-3' Tm: 59.038
Reverse Primer: 5'-CCCATAGCTCTTTGGTCAGGTC-3' Tm: 59.258
Expected PCR Product Length: 399
Expected CelI Cleavage Product Sizes: 224, 175
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr4:115491578-115491635

Rank: 100	Homology Score: 2
	Genomic Region: Intergenic	Closest Gene: ADTRP
Match Type: R14L
	          Right Nuclease
	         TCCCCTCCACCCCACAGT
	5'-TAGCT TCCCTCCCACCCCCCACC       CTGGAACAACAGAT       TGGGTTAGTGCTAGAAAA TGTCT-3'
	3'-ATCGA AGGGAGGGTGGGGGGTGG       GACCTTGTTGTCTA       ACCCAATCACGATCTTTT ACAGA-5'
	                                                       TCCTAACCACTGTCTTTT
	                                                             Left Nuclease
Warning! Primer pair was generated using suboptimal parameters. 
Primer Index: 006-011853744
Forward Primer: 5'-GTCTACGGATCTAACACTCCAAAAATCTTAGC-3' Tm: 59.848
Reverse Primer: 5'-CTAGGGGGCTGAAATGGAACAAC-3' Tm: 59.148
Expected PCR Product Length: 325
Expected CelI Cleavage Product Sizes: 172, 153
In Silico PCR
View in Genome Browser, Chromosomal Coordinates - chr6:11853889-11853938

Plate format Oligo Order Form
Well
Name
Sequence
5' mods
Internal
Notes
Primer Plate #1
A1
019-055627003F
CCAGGAAATGGGGGTGTGTC
Hockemeyer_AAVS1_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
A2
019-055627003R
GTCCACTTCAGGACAGCATGTTTG
Hockemeyer_AAVS1_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
B1
007-115754830F
CGGTTGAGACTGAAGCATGCAAAG
Hockemeyer_AAVS1_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
B2
007-115754830R
AAGGACCACACTACTGACATTAGATCTACC
Hockemeyer_AAVS1_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
C1
013-103511881F
TCTCTGGCTGGCAGTTGAGG
Hockemeyer_AAVS1_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
C2
013-103511881R
CAGGTCACTGAAAACTGCAAACCG
Hockemeyer_AAVS1_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
D1
002-001827851F
GGGGGTTGGGGAACGTTTTTAG
Hockemeyer_AAVS1_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
D2
002-001827851R
TCAGGACGAGAGCAGCTCAAATG
Hockemeyer_AAVS1_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
E1
001-024697363F
AGCCTCCCAAAGTGCTAGGAC
Hockemeyer_AAVS1_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
E2
001-024697363R
GTGGAGAACATCCATTCACAGGG
Hockemeyer_AAVS1_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
F1
003-070995805F
GCCGTTGCTTTTGCCTTGAACATC
Hockemeyer_AAVS1_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
F2
003-070995805R
TACTTGAGTCCCTGGATCTGGC
Hockemeyer_AAVS1_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
G1
001-062633493F
TCCTCCTGCCTAATGGCTTTACTG
Hockemeyer_AAVS1_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
G2
001-062633493R
GCTACTGAGCCCAGCCTAATTTAC
Hockemeyer_AAVS1_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
H1
006-021198081F
CCGTGATTTGAACAGGGTTCGC
Hockemeyer_AAVS1_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
H2
006-021198081R
CTCTCCCCTTTAAATGCCCTTTGC
Hockemeyer_AAVS1_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
A3
006-052101450F
GCTGGGAATGCAAACAAACACCTG
Hockemeyer_AAVS1_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
A4
006-052101450R
TCATCCACCATGTGCAGTAAGAGG
Hockemeyer_AAVS1_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
B3
011-119574363F
CAACGAGGCTGACGCTCTTTC
Hockemeyer_AAVS1_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
B4
011-119574363R
CTGAGCACCTGTGTGCCAGT
Hockemeyer_AAVS1_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
C3
003-124702567F
TGCAAGTCTACAACCAGGGTGTG
Hockemeyer_AAVS1_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
C4
003-124702567R
CAGAAGGCAGCTGGAAGTCTC
Hockemeyer_AAVS1_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
D3
003-135693383F
CTGTGCTTGCTTTGTCCCAGTC
Hockemeyer_AAVS1_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
D4
003-135693383R
CCTTGCTAGGATTCCTAAGCCC
Hockemeyer_AAVS1_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
E3
009-138652006F
GGGAAGGGTCAAGGTGGACA
Hockemeyer_AAVS1_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
E4
009-138652006R
AATAGCAGGGTCCCTCCAGGGT
Hockemeyer_AAVS1_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
F3
008-145493530F
GAGCCATTAAGGGCTAGAGGG
Hockemeyer_AAVS1_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
F4
008-145493530R
GGAAAGCTCCCAAGAAGGGAAG
Hockemeyer_AAVS1_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
G3
004-148942680F
GTAGGTAAGCCAGGGAGCTGT
Hockemeyer_AAVS1_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
G4
004-148942680R
CCTGCCAACACCATGTTTCCAC
Hockemeyer_AAVS1_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
H3
007-158192599F
TAGTCCCCAGGCACTTGCAG
Hockemeyer_AAVS1_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
H4
007-158192599R
GCCTCGTAAGTTTAGGCATCCTC
Hockemeyer_AAVS1_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
A5
010-024751913F
GGTGGATGTGCAGAGATGGAG
Hockemeyer_AAVS1_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
A6
010-024751913R
CTCTACTGAGCCCTCCAGTG
Hockemeyer_AAVS1_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
B5
00X-031479613F
GTCTGGACATGTCTGAATGCCTAG
Hockemeyer_AAVS1_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
B6
00X-031479613R
GGTTAGGTAAAGTGTCCCCTGC
Hockemeyer_AAVS1_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
C5
010-038264363F
GGTGCTTACGAAATGCCTAAGCTC
Hockemeyer_AAVS1_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
C6
010-038264363R
AGGCCTTCCTGCATCCTCCA
Hockemeyer_AAVS1_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
D5
020-050029460F
AGAAGCACAGGATGAGGCCG
Hockemeyer_AAVS1_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
D6
020-050029460R
CAGTGGATTGACAGAGGCAGATG
Hockemeyer_AAVS1_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
E5
003-056061208F
GGCATGGATATCCAGGCTTGCT
Hockemeyer_AAVS1_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
E6
003-056061208R
TGGGGATGTACAGAGGCCAG
Hockemeyer_AAVS1_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
F5
004-088055877F
TGGGGTGTTTGGATTGGGAGG
Hockemeyer_AAVS1_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
F6
004-088055877R
TCAGCTAGCATTTCAGCTCAGGC
Hockemeyer_AAVS1_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
G5
010-093286041F
GATTCTGAGCAGGGAGCTTGG
Hockemeyer_AAVS1_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
G6
010-093286041R
ACAGGATGAGCTAGGGCCTGA
Hockemeyer_AAVS1_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
H5
010-096170810F
GGCATGCCAGAGATAGGACTATTC
Hockemeyer_AAVS1_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
H6
010-096170810R
GTGCTACAGTGGCAACCTTCATG
Hockemeyer_AAVS1_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
A7
002-096866458F
CGCCGAAACTGGCCTCAAATTCC
Hockemeyer_AAVS1_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
A8
002-096866458R
CTGCCTAGCAGTTACCTTTGTGG
Hockemeyer_AAVS1_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
B7
011-011075984F
GCCTCATTACTGCTAGGTGGAAG
Hockemeyer_AAVS1_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
B8
011-011075984R
CACCCTAACATCCAGGCATGATGA
Hockemeyer_AAVS1_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
C7
008-145324445F
GAGCCATTAAGGGCTAGAGGG
Hockemeyer_AAVS1_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
C8
008-145324445R
GGAAAGCTCCCAAGAAGGGAAG
Hockemeyer_AAVS1_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
D7
005-157580667F
GTAATCCAGGGTCTCTGTTTGTCC
Hockemeyer_AAVS1_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
D8
005-157580667R
GTCTTTGGGTGGCATTTGTGAGG
Hockemeyer_AAVS1_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
E7
001-163803301F
GGTTAAGTGCTTGACCACTGCTG
Hockemeyer_AAVS1_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
E8
001-163803301R
CACCATGGCACATGTCTACTTGAG
Hockemeyer_AAVS1_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
F7
001-198360906F
GGTTTTATAGCCCTTCTCCTGCC
Hockemeyer_AAVS1_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
F8
001-198360906R
GTTGTGGGATTTGAGCTGGTGG
Hockemeyer_AAVS1_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
G7
008-024031178F
AGTGAGGAGGGGCCTGAAGT
Hockemeyer_AAVS1_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
G8
008-024031178R
CATTTGTTTCGCAGGCTAGCTCC
Hockemeyer_AAVS1_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
H7
012-030180063F
CTATAAGGCTGCTGGGCCTTTC
Hockemeyer_AAVS1_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
H8
012-030180063R
GAGGCAAACCTACTTTATGGAGGG
Hockemeyer_AAVS1_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
A9
012-003414995F
TGACGTTCCCTGGGCCAAGA
Hockemeyer_AAVS1_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
A10
012-003414995R
AAAATCCAGTTCCTTGCTCGCACC
Hockemeyer_AAVS1_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
B9
017-035084330F
GAGCCTCTGCTGGTTGGATG
Hockemeyer_AAVS1_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
B10
017-035084330R
ATGTTGGGTGCTGGGGAAGG
Hockemeyer_AAVS1_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
C9
021-036773953F
CCAGAGCGGTTTTCCAAGATGC
Hockemeyer_AAVS1_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
C10
021-036773953R
GGATTCACAACACCATGGATGGAC
Hockemeyer_AAVS1_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
D9
003-039683322F
GGTCTCCAGGGAGCATTATACAG
Hockemeyer_AAVS1_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
D10
003-039683322R
CTAGACACAGAGCCTTCAGTAAGC
Hockemeyer_AAVS1_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
E9
020-058618528F
GGGAGCTATGATCATGCCATAGG
Hockemeyer_AAVS1_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
E10
020-058618528R
GCTCCATGTCATTGTTCTGGGC
Hockemeyer_AAVS1_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
F9
011-060790178F
GCCTGTGCTGAGACTGGGAA
Hockemeyer_AAVS1_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
F10
011-060790178R
AGCTGTCAGTCAAATTGCTGGGTG
Hockemeyer_AAVS1_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
G9
015-067305574F
CCCCACCTTGCTGAATGGAC
Hockemeyer_AAVS1_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
G10
015-067305574R
CCAGTCCTTGATCTCTTCCTGAGT
Hockemeyer_AAVS1_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
H9
00X-068408586F
GCACTCCTTTACCCTCCGATATC
Hockemeyer_AAVS1_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
H10
00X-068408586R
AGGTTTGAGGTGCACTACTTCAGG
Hockemeyer_AAVS1_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
A11
002-113984626F
GGGAAGTCTATGAATAGGGCACAG
Hockemeyer_AAVS1_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
A12
002-113984626R
TGATACATATGGGAGCCCCCATGG
Hockemeyer_AAVS1_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
B11
001-185833320F
CATCGTGTAAGAGAAGGCAGAGTG
Hockemeyer_AAVS1_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
B12
001-185833320R
CTGTTGGATAAGTTGCAGCACCTC
Hockemeyer_AAVS1_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
C11
017-036923296F
CCTTTCTTCTTGGGAGACCCAAC
Hockemeyer_AAVS1_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
C12
017-036923296R
GAGTTTGGCCCCCAATTTTTGGCA
Hockemeyer_AAVS1_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
D11
005-054696084F
CGGCATCCCCTTATTAGACCC
Hockemeyer_AAVS1_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
D12
005-054696084R
CTGACAGGTTCAGAGCTGACAGT
Hockemeyer_AAVS1_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
E11
017-008242168F
CAGCTATTGCGGGGAGCTAATG
Hockemeyer_AAVS1_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
E12
017-008242168R
AGGCCACAGCTCAGAACTGTGGA
Hockemeyer_AAVS1_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
F11
002-103259635F
GTTCTCTGCGTTCTTTACGTCGTC
Hockemeyer_AAVS1_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
F12
002-103259635R
GTCAACCTCACAAGGCACAGAAG
Hockemeyer_AAVS1_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
G11
004-106759798F
GAGACCCACAGAGAATTGTCTTCC
Hockemeyer_AAVS1_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
G12
004-106759798R
AGGTGTGAGTAGCACATAAGGTGG
Hockemeyer_AAVS1_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
H11
00X-106962065F
CTCTCTGAACACGGTGTTTGCACT
Hockemeyer_AAVS1_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
H12
00X-106962065R
TGCCTCTGTCTCCACATAAGGG
Hockemeyer_AAVS1_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
Primer Plate #2
A1
006-107832934F
CCCTTTTCTCTGGAGAAGGGGA
Hockemeyer_AAVS1_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
A2
006-107832934R
GGCTTGCCTATATGGCAAAGCCT
Hockemeyer_AAVS1_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
B1
008-108496180F
GATGGGAAAGATGCCTAGGAAGC
Hockemeyer_AAVS1_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
B2
008-108496180R
GGAAACTGAGGGCCACTAAAGTC
Hockemeyer_AAVS1_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
C1
002-115914630F
GTGGGTAAACATGACAACAGCAGG
Hockemeyer_AAVS1_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
C2
002-115914630R
CTTGCTCTCTGGAGACCTGAAGAT
Hockemeyer_AAVS1_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
D1
011-126543310F
CCAGCAGCTCTGGAAATTGGATC
Hockemeyer_AAVS1_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
D2
011-126543310R
GAGGAAGATCTTACAGACCCAACC
Hockemeyer_AAVS1_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
E1
008-129090046F
CATCCTCCCAGCCATCAATACC
Hockemeyer_AAVS1_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
E2
008-129090046R
AGGCTGGGACCACAGGCATA
Hockemeyer_AAVS1_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
F1
012-129882479F
GGCCAAAACCTCTCTACATGGG
Hockemeyer_AAVS1_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
F2
012-129882479R
CAGGGAGGAAAACAGGCACTAAAG
Hockemeyer_AAVS1_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
G1
012-129962958F
CCACCATCTCATGTATGGTTGAGC
Hockemeyer_AAVS1_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
G2
012-129962958R
GGGGATCAGAGCACATTCCAG
Hockemeyer_AAVS1_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
H1
00X-132539743F
CTGCCATAGACTGGGAAACTGGA
Hockemeyer_AAVS1_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
H2
00X-132539743R
CCAGAACACAGCTGGGAATGTGGA
Hockemeyer_AAVS1_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
A3
009-133706117F
GGCACTCTCCCTACATGATTCC
Hockemeyer_AAVS1_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
A4
009-133706117R
GTGGGTCCAGTGTATGTCCAAC
Hockemeyer_AAVS1_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
B3
002-135020983F
GGGCACAAGTTCTGGTGAAAGC
Hockemeyer_AAVS1_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
B4
002-135020983R
GTCATGTAAGGCAATACCCACCAG
Hockemeyer_AAVS1_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
C3
006-136482738F
GGGAGGGAACCCATTGCAGT
Hockemeyer_AAVS1_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
C4
006-136482738R
CTATTGAGGGCAGGGAGCGT
Hockemeyer_AAVS1_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
D3
005-137303124F
ACTGCTTGAACCCAGGAGGC
Hockemeyer_AAVS1_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
D4
005-137303124R
CTTTGTTGCCTGGGCTGGTC
Hockemeyer_AAVS1_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
E3
010-014726614F
GCATTGTGCCAAAAGGATCCCG
Hockemeyer_AAVS1_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
E4
010-014726614R
GATAGGGAGACCCCATGTCTAC
Hockemeyer_AAVS1_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
F3
002-149982161F
GGCATCTTCGAGTACCTTAACTCC
Hockemeyer_AAVS1_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
F4
002-149982161R
CTCTTCTCAAGTTCAAGGGTCAGTTGTAC
Hockemeyer_AAVS1_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
G3
004-159462895F
GCAAGCACTTGAGCATGTATCTCC
Hockemeyer_AAVS1_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
G4
004-159462895R
AACCCAGGAGGTGGAGGTTG
Hockemeyer_AAVS1_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
H3
013-020219809F
TCGCTTGAACCCAGGAGGCA
Hockemeyer_AAVS1_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
H4
013-020219809R
CCCTGGATCCTGGAATGTCCT
Hockemeyer_AAVS1_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
A5
017-021147767F
CCATGCCTAGAACACTTGAGCC
Hockemeyer_AAVS1_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
A6
017-021147767R
CTTTCCTGCTGCGTCTCTCTC
Hockemeyer_AAVS1_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
B5
006-021174562F
GTCGGTATAATCTTAGTCAAAAGTTCAACAGGG
Hockemeyer_AAVS1_Ranking-Homology_5T	Warning! Primer pair was generated using suboptimal parameters. 
B6
006-021174562R
TAGTTAAATTGCCCTCCAAAATGGCCTTATCAATG
Hockemeyer_AAVS1_Ranking-Homology_5T	Warning! Primer pair was generated using suboptimal parameters. 
C5
001-226575130F
GGAGACAAAAATGCCAGCCCTATC
Hockemeyer_AAVS1_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
C6
001-226575130R
CCACTGTGGCACATCTGTGTC
Hockemeyer_AAVS1_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
D5
001-024882812F
CATCTGTTGGAGGAAGTGCCTG
Hockemeyer_AAVS1_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
D6
001-024882812R
CTCTGCATAGGGCAGACCCA
Hockemeyer_AAVS1_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
E5
022-026293769F
GTTTAAGGCCGTGTGCATTCTGG
Hockemeyer_AAVS1_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
E6
022-026293769R
GTTTGCTCAGAGGTAAAGTGGGG
Hockemeyer_AAVS1_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
F5
017-031174782F
CTCAGCCTCCCAAAATGCTGAGA
Hockemeyer_AAVS1_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
F6
017-031174782R
CGGAGAGAGGCCCATTTTTCATAG
Hockemeyer_AAVS1_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
G5
010-000333834F
TCCCAACCTTTAGCTAGGTGTGGC
Hockemeyer_AAVS1_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
G6
010-000333834R
TAGGCATCTCCTCCTCCATGAAG
Hockemeyer_AAVS1_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
H5
016-033560648F
GTTCTTTTGAAAAGGGTACTGCCAAAGAGC
Hockemeyer_AAVS1_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
H6
016-033560648R
GGTTGGAGATGCAGACGTAGAATG
Hockemeyer_AAVS1_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
A7
015-033799697F
GGCACTGTGGCCAATTTTATGGCT
Hockemeyer_AAVS1_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
A8
015-033799697R
CCCAGTCCTACGAGATAGATTGC
Hockemeyer_AAVS1_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
B7
006-000344885F
ACCTTCCTCACCTGCGTCAC
Hockemeyer_AAVS1_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
B8
006-000344885R
AACCTGGTCATACTGTCTGTGGC
Hockemeyer_AAVS1_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
C7
002-037018108F
TTTCAGCTGCATCCCGGACC
Hockemeyer_AAVS1_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
C8
002-037018108R
AGGAGATGCGGCCAAGTCAG
Hockemeyer_AAVS1_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
D7
002-043471241F
GCCCACCTCAAAACACTGGGTA
Hockemeyer_AAVS1_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
D8
002-043471241R
GTGAGGTGCACACAAAACTGGG
Hockemeyer_AAVS1_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
E7
017-044029096F
GGACGGACTCAGGAGGCAAT
Hockemeyer_AAVS1_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
E8
017-044029096R
CACACCAAGGCTGTTTTCCTCAGA
Hockemeyer_AAVS1_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
F7
021-046316063F
GATCCTGCCTGGTGGATTTCAAG
Hockemeyer_AAVS1_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
F8
021-046316063R
ATGTGTGAGCATGGGGGAGG
Hockemeyer_AAVS1_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
G7
016-050647321F
TCATGGGAAGGCAGCAGGGA
Hockemeyer_AAVS1_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
G8
016-050647321R
TAAAGCCTCTCCGGTATCTGACAC
Hockemeyer_AAVS1_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
H7
014-053388076F
GGTTTTGGCAAAGTCCTGCCAATC
Hockemeyer_AAVS1_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
H8
014-053388076R
CCTACCGATCATTCAGTTCAGCTC
Hockemeyer_AAVS1_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
A9
003-056450116F
GGGAAAAGACGTGATTTGGCCAAC
Hockemeyer_AAVS1_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
A10
003-056450116R
GGAGGTTAGCTTTGAAGGGTGG
Hockemeyer_AAVS1_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
B9
005-065282040F
GCTGCTTACTCAGTGTCCTACTC
Hockemeyer_AAVS1_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
B10
005-065282040R
GGTATAGGACTAACGTGTTTAACGTGTGTG
Hockemeyer_AAVS1_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
C9
001-065337739F
TCTCTCCTTGCAGCTCTACTGG
Hockemeyer_AAVS1_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
C10
001-065337739R
CTTCCATGTAGAGTGTCTCAGCAG
Hockemeyer_AAVS1_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
D9
007-066670329F
TGACTGTTCCCCCCTTAGACTC
Hockemeyer_AAVS1_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
D10
007-066670329R
TTGACTCCTACTACCAGGGCG
Hockemeyer_AAVS1_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
E9
015-071318700F
GTGCTGCAATAAACATACATGTGCATGTGTC
Hockemeyer_AAVS1_Ranking-Homology_5T	Warning! Primer pair was generated using suboptimal parameters. 
E10
015-071318700R
CCCCATCAAAAAGTGGGTGAAGG
Hockemeyer_AAVS1_Ranking-Homology_5T	Warning! Primer pair was generated using suboptimal parameters. 
F9
007-072071381F
ACTCCTACTACCAGGGCGGTAT
Hockemeyer_AAVS1_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
F10
007-072071381R
TTGTGACTGTTCCCCCCTTAGAC
Hockemeyer_AAVS1_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
G9
002-072909053F
CCCTTCTCTCCACTAGCCAAGAAA
Hockemeyer_AAVS1_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
G10
002-072909053R
CTTAAGAAGAATGAGGAAATGGTTGGAGCC
Hockemeyer_AAVS1_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
H9
011-075336327F
CATTCAGCTGAGCAAACAAAGCCC
Hockemeyer_AAVS1_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
H10
011-075336327R
CCTCTCTGGGCTTTGGTTTTCTC
Hockemeyer_AAVS1_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
A11
009-077165385F
GGTCTTATCGACTAGCTAGGGTTG
Hockemeyer_AAVS1_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
A12
009-077165385R
GGGCTGCCCTTTAGATTTTGCTG
Hockemeyer_AAVS1_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
B11
016-081100645F
GCCCCCAAAGTGCATGTTTGGA
Hockemeyer_AAVS1_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
B12
016-081100645R
GAGCTCTCCGATAACATCATGGC
Hockemeyer_AAVS1_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
C11
ap1-000838311F
ACCAAGGCTGTTTTCCTCAGACC
Hockemeyer_AAVS1_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
C12
ap1-000838311R
AACCACAGGGACGGACTCAG
Hockemeyer_AAVS1_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
D11
004-090656661F
CAGGGGAGTGTGTACATAGTGTAG
Hockemeyer_AAVS1_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
D12
004-090656661R
CCAGAGTTGTTCCTCTCCACATAG
Hockemeyer_AAVS1_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
E11
015-093472933F
GGTGTGGATCCCGTTGACAATCT
Hockemeyer_AAVS1_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
E12
015-093472933R
GCCAAGTGCAGGTTTGCTTACAG
Hockemeyer_AAVS1_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
F11
002-097793909F
GGTTGGAGATGCAGACGTAGAATG
Hockemeyer_AAVS1_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
F12
002-097793909R
GAAAAGGGTACCGCCAAAGAGC
Hockemeyer_AAVS1_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
G11
010-097970211F
GGGCTTTCGCCGACAGGTTT
Hockemeyer_AAVS1_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
G12
010-097970211R
AGGCAGCTAGGGGATGCTGA
Hockemeyer_AAVS1_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
H11
003-109619335F
CAGGGAAGTCTCAGTGGTTTTTGG
Hockemeyer_AAVS1_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
H12
003-109619335R
CCAGGTGTATTCTGTGTGGCTAG
Hockemeyer_AAVS1_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
Primer Plate #3
A1
004-112705369F
CATCCTTACTCAGGGCTGTTTCATGATGT
Hockemeyer_AAVS1_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
A2
004-112705369R
CTGGACAACAGACCTTTCCATGG
Hockemeyer_AAVS1_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
B1
012-011342495F
CTGTCTTCCAGTCTAAAGCAACTACCTC
Hockemeyer_AAVS1_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
B2
012-011342495R
CCACACATCCTCTTCCTCCTATTC
Hockemeyer_AAVS1_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
C1
004-115491385F
CATGTTGCACAGGCTGAGTACTCT
Hockemeyer_AAVS1_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
C2
004-115491385R
CCCATAGCTCTTTGGTCAGGTC
Hockemeyer_AAVS1_Ranking-Homology_5T	Primer pair generated using optimal parameters. 
D1
006-011853744F
GTCTACGGATCTAACACTCCAAAAATCTTAGC
Hockemeyer_AAVS1_Ranking-Homology_5T	Warning! Primer pair was generated using suboptimal parameters. 
D2
006-011853744R
CTAGGGGGCTGAAATGGAACAAC
Hockemeyer_AAVS1_Ranking-Homology_5T	Warning! Primer pair was generated using suboptimal parameters.